AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1089990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1089990 MtrunA17_Chr4g0014461 87.500 80 10 0 1 80 1 80 1.64e-47 145
Msa1089990 MtrunA17_Chr4g0014431 81.013 79 15 0 1 79 1 79 5.32e-42 131
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTAATTATGTTGTAATA+TGG 0.214329 7_3:+3599330 Msa1089990:three_prime_UTR
TAATATTATTTACATAATTA+TGG 0.219522 7_3:+3599303 Msa1089990:three_prime_UTR
ACTCCTATTTGTATGTAATA+TGG 0.281587 7_3:+3599354 Msa1089990:three_prime_UTR
AGGTGTTTATCACCTGTATA+AGG 0.302748 7_3:-3599130 None:intergenic
ATTTGTATGTAATATGGATA+TGG 0.337532 7_3:+3599360 Msa1089990:three_prime_UTR
ATCCTTCTTGTCTTCGTTTG+TGG 0.338806 7_3:+3597663 Msa1089990:CDS
TATCCATATTACATACAAAT+AGG 0.368185 7_3:-3599357 None:intergenic
ATGTAATATGGATATGGATA+TGG 0.371160 7_3:+3599366 Msa1089990:three_prime_UTR
AGCAAAATGGCTTTAAAGGT+TGG 0.418570 7_3:+3597636 None:intergenic
AATTGTTGCATTGCATCAAC+TGG 0.473599 7_3:+3599166 Msa1089990:CDS
TACATGACTTGCCCTTATAC+AGG 0.473979 7_3:+3599118 Msa1089990:CDS
GGTGTTTATCACCTGTATAA+GGG 0.520757 7_3:-3599129 None:intergenic
TACCACAAACGAAGACAAGA+AGG 0.582604 7_3:-3597665 None:intergenic
GCAATGCAACAATTGCATGC+TGG 0.616125 7_3:-3599157 None:intergenic
TGTGTACTTTATGAAGCAGA+TGG 0.632102 7_3:+3599190 Msa1089990:CDS
AATATGGATATGGATATGGA+TGG 0.636950 7_3:+3599370 Msa1089990:three_prime_UTR
CTGAATCATAGCAAAACTGA+GGG 0.651830 7_3:-3599090 None:intergenic
ACAAACGAAGACAAGAAGGA+TGG 0.658438 7_3:-3597661 None:intergenic
ACTGAATCATAGCAAAACTG+AGG 0.661702 7_3:-3599091 None:intergenic
AACAATTGCATGCTGGATCG+AGG 0.699789 7_3:-3599150 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTGTTGAATTGTAT+AGG - chr7_3:3598895-3598914 None:intergenic 15.0%
!! GAATTAATTATGTTGTAATA+TGG + chr7_3:3599330-3599349 Msa1089990:three_prime_UTR 15.0%
!!! ATTTTTTTGTTGAATTGTAT+AGG - chr7_3:3598895-3598914 None:intergenic 15.0%
!! GAATTAATTATGTTGTAATA+TGG + chr7_3:3599330-3599349 Msa1089990:three_prime_UTR 15.0%
!!! TTGTTTCTTTTAGCAATTTA+TGG + chr7_3:3597727-3597746 Msa1089990:intron 20.0%
!!! TAAACAAACTGATTTATGTT+TGG + chr7_3:3598009-3598028 Msa1089990:intron 20.0%
!! TAAACAAACTTTAACTAAAC+CGG + chr7_3:3598425-3598444 Msa1089990:intron 20.0%
!! TATCCATATTACATACAAAT+AGG - chr7_3:3599360-3599379 None:intergenic 20.0%
!! ATTTGTATGTAATATGGATA+TGG + chr7_3:3599360-3599379 Msa1089990:three_prime_UTR 20.0%
!!! TTGTTTCTTTTAGCAATTTA+TGG + chr7_3:3597727-3597746 Msa1089990:intron 20.0%
!!! TAAACAAACTGATTTATGTT+TGG + chr7_3:3598009-3598028 Msa1089990:intron 20.0%
!! TAAACAAACTTTAACTAAAC+CGG + chr7_3:3598425-3598444 Msa1089990:intron 20.0%
!! TATCCATATTACATACAAAT+AGG - chr7_3:3599360-3599379 None:intergenic 20.0%
!! ATTTGTATGTAATATGGATA+TGG + chr7_3:3599360-3599379 Msa1089990:three_prime_UTR 20.0%
!! TGAGAGAATTGATTTATGTT+TGG + chr7_3:3597965-3597984 Msa1089990:intron 25.0%
! ACTAAAATAGACATAGCTAA+AGG + chr7_3:3598068-3598087 Msa1089990:intron 25.0%
! TAAAGGAGAATTGATAAAGA+AGG + chr7_3:3598085-3598104 Msa1089990:intron 25.0%
! TAAAGAAGGTGAATTGATAA+AGG + chr7_3:3598099-3598118 Msa1089990:intron 25.0%
! AAAGAAGGTGAATTGATAAA+GGG + chr7_3:3598100-3598119 Msa1089990:intron 25.0%
! GGGCAATAAAGAAAAATAAT+AGG + chr7_3:3598120-3598139 Msa1089990:intron 25.0%
!!! TTTTTAAGAGGACTACAAAT+TGG + chr7_3:3598364-3598383 Msa1089990:intron 25.0%
! CTTAATAATTAGACCTTGTA+AGG - chr7_3:3598478-3598497 None:intergenic 25.0%
! AACTTCATACCTAATTAAGT+TGG + chr7_3:3598735-3598754 Msa1089990:intron 25.0%
!!! TTATTTTTGTTGCAGTACAA+GGG - chr7_3:3598786-3598805 None:intergenic 25.0%
!!! TTTATTTTTGTTGCAGTACA+AGG - chr7_3:3598787-3598806 None:intergenic 25.0%
!! TGTACTGCTGATTAAAAATA+TGG + chr7_3:3599223-3599242 Msa1089990:exon 25.0%
! ACTCCTATTTGTATGTAATA+TGG + chr7_3:3599354-3599373 Msa1089990:three_prime_UTR 25.0%
! ATGTAATATGGATATGGATA+TGG + chr7_3:3599366-3599385 Msa1089990:three_prime_UTR 25.0%
!! TGAGAGAATTGATTTATGTT+TGG + chr7_3:3597965-3597984 Msa1089990:intron 25.0%
! ACTAAAATAGACATAGCTAA+AGG + chr7_3:3598068-3598087 Msa1089990:intron 25.0%
! TAAAGGAGAATTGATAAAGA+AGG + chr7_3:3598085-3598104 Msa1089990:intron 25.0%
! TAAAGAAGGTGAATTGATAA+AGG + chr7_3:3598099-3598118 Msa1089990:intron 25.0%
! AAAGAAGGTGAATTGATAAA+GGG + chr7_3:3598100-3598119 Msa1089990:intron 25.0%
! GGGCAATAAAGAAAAATAAT+AGG + chr7_3:3598120-3598139 Msa1089990:intron 25.0%
!!! TTTTTAAGAGGACTACAAAT+TGG + chr7_3:3598364-3598383 Msa1089990:intron 25.0%
! CTTAATAATTAGACCTTGTA+AGG - chr7_3:3598478-3598497 None:intergenic 25.0%
! AACTTCATACCTAATTAAGT+TGG + chr7_3:3598735-3598754 Msa1089990:intron 25.0%
!!! TTATTTTTGTTGCAGTACAA+GGG - chr7_3:3598786-3598805 None:intergenic 25.0%
!!! TTTATTTTTGTTGCAGTACA+AGG - chr7_3:3598787-3598806 None:intergenic 25.0%
!! TGTACTGCTGATTAAAAATA+TGG + chr7_3:3599223-3599242 Msa1089990:exon 25.0%
! ACTCCTATTTGTATGTAATA+TGG + chr7_3:3599354-3599373 Msa1089990:three_prime_UTR 25.0%
! ATGTAATATGGATATGGATA+TGG + chr7_3:3599366-3599385 Msa1089990:three_prime_UTR 25.0%
CAATATGTAGAAAGAAAGAG+AGG - chr7_3:3597704-3597723 None:intergenic 30.0%
!!! TTTTTGGCTAGAATTGCTTT+TGG + chr7_3:3598275-3598294 Msa1089990:intron 30.0%
! ACCATACTTTATTTGAGATG+TGG + chr7_3:3598335-3598354 Msa1089990:intron 30.0%
ACCACATCTCAAATAAAGTA+TGG - chr7_3:3598339-3598358 None:intergenic 30.0%
!!! ATGTGGTGAACTTTTTTAAG+AGG + chr7_3:3598352-3598371 Msa1089990:intron 30.0%
TATTTAGAAACTAAGTGCTC+CGG - chr7_3:3598447-3598466 None:intergenic 30.0%
AAATAACAATCCTCCTTACA+AGG + chr7_3:3598462-3598481 Msa1089990:intron 30.0%
AATAATTAGACCTTGTAAGG+AGG - chr7_3:3598475-3598494 None:intergenic 30.0%
! AACTTAAACAACTTTTTGCG+TGG + chr7_3:3598542-3598561 Msa1089990:intron 30.0%
CATAAACAGAAACTCAAGAT+AGG + chr7_3:3598702-3598721 Msa1089990:intron 30.0%
CAATTGTGTCCAACTTAATT+AGG - chr7_3:3598747-3598766 None:intergenic 30.0%
!! CTAATTAAGTTGGACACAAT+TGG + chr7_3:3598745-3598764 Msa1089990:intron 30.0%
! AACTTTCTTGTTTATGATCC+AGG + chr7_3:3599016-3599035 Msa1089990:intron 30.0%
AATATGGATATGGATATGGA+TGG + chr7_3:3599370-3599389 Msa1089990:three_prime_UTR 30.0%
CAATATGTAGAAAGAAAGAG+AGG - chr7_3:3597704-3597723 None:intergenic 30.0%
!!! TTTTTGGCTAGAATTGCTTT+TGG + chr7_3:3598275-3598294 Msa1089990:intron 30.0%
! ACCATACTTTATTTGAGATG+TGG + chr7_3:3598335-3598354 Msa1089990:intron 30.0%
ACCACATCTCAAATAAAGTA+TGG - chr7_3:3598339-3598358 None:intergenic 30.0%
!!! ATGTGGTGAACTTTTTTAAG+AGG + chr7_3:3598352-3598371 Msa1089990:intron 30.0%
TATTTAGAAACTAAGTGCTC+CGG - chr7_3:3598447-3598466 None:intergenic 30.0%
AAATAACAATCCTCCTTACA+AGG + chr7_3:3598462-3598481 Msa1089990:intron 30.0%
AATAATTAGACCTTGTAAGG+AGG - chr7_3:3598475-3598494 None:intergenic 30.0%
! AACTTAAACAACTTTTTGCG+TGG + chr7_3:3598542-3598561 Msa1089990:intron 30.0%
CATAAACAGAAACTCAAGAT+AGG + chr7_3:3598702-3598721 Msa1089990:intron 30.0%
CAATTGTGTCCAACTTAATT+AGG - chr7_3:3598747-3598766 None:intergenic 30.0%
!! CTAATTAAGTTGGACACAAT+TGG + chr7_3:3598745-3598764 Msa1089990:intron 30.0%
! AACTTTCTTGTTTATGATCC+AGG + chr7_3:3599016-3599035 Msa1089990:intron 30.0%
AATATGGATATGGATATGGA+TGG + chr7_3:3599370-3599389 Msa1089990:three_prime_UTR 30.0%
! TTCTAGAAAAGCTAGAAGCT+AGG + chr7_3:3598182-3598201 Msa1089990:intron 35.0%
CAAAAACAACGTGTTTGGTT+TGG - chr7_3:3598261-3598280 None:intergenic 35.0%
!! CAAACCAAACACGTTGTTTT+TGG + chr7_3:3598259-3598278 Msa1089990:intron 35.0%
! GCACTGTAGAGAATTGTTTT+TGG + chr7_3:3598297-3598316 Msa1089990:intron 35.0%
!!! TGTAGAGAATTGTTTTTGGC+TGG + chr7_3:3598301-3598320 Msa1089990:intron 35.0%
!!! GTAGAGAATTGTTTTTGGCT+GGG + chr7_3:3598302-3598321 Msa1089990:intron 35.0%
GTTTCGTCTTCAGCTTAATT+AGG - chr7_3:3598873-3598892 None:intergenic 35.0%
CTGAATCATAGCAAAACTGA+GGG - chr7_3:3599093-3599112 None:intergenic 35.0%
ACTGAATCATAGCAAAACTG+AGG - chr7_3:3599094-3599113 None:intergenic 35.0%
GGTGTTTATCACCTGTATAA+GGG - chr7_3:3599132-3599151 None:intergenic 35.0%
AGGTGTTTATCACCTGTATA+AGG - chr7_3:3599133-3599152 None:intergenic 35.0%
AATTGTTGCATTGCATCAAC+TGG + chr7_3:3599166-3599185 Msa1089990:CDS 35.0%
TGTGTACTTTATGAAGCAGA+TGG + chr7_3:3599190-3599209 Msa1089990:CDS 35.0%
! TTCTAGAAAAGCTAGAAGCT+AGG + chr7_3:3598182-3598201 Msa1089990:intron 35.0%
CAAAAACAACGTGTTTGGTT+TGG - chr7_3:3598261-3598280 None:intergenic 35.0%
!! CAAACCAAACACGTTGTTTT+TGG + chr7_3:3598259-3598278 Msa1089990:intron 35.0%
! GCACTGTAGAGAATTGTTTT+TGG + chr7_3:3598297-3598316 Msa1089990:intron 35.0%
!!! TGTAGAGAATTGTTTTTGGC+TGG + chr7_3:3598301-3598320 Msa1089990:intron 35.0%
!!! GTAGAGAATTGTTTTTGGCT+GGG + chr7_3:3598302-3598321 Msa1089990:intron 35.0%
GTTTCGTCTTCAGCTTAATT+AGG - chr7_3:3598873-3598892 None:intergenic 35.0%
CTGAATCATAGCAAAACTGA+GGG - chr7_3:3599093-3599112 None:intergenic 35.0%
ACTGAATCATAGCAAAACTG+AGG - chr7_3:3599094-3599113 None:intergenic 35.0%
GGTGTTTATCACCTGTATAA+GGG - chr7_3:3599132-3599151 None:intergenic 35.0%
AGGTGTTTATCACCTGTATA+AGG - chr7_3:3599133-3599152 None:intergenic 35.0%
AATTGTTGCATTGCATCAAC+TGG + chr7_3:3599166-3599185 Msa1089990:CDS 35.0%
TGTGTACTTTATGAAGCAGA+TGG + chr7_3:3599190-3599209 Msa1089990:CDS 35.0%
ACAAACGAAGACAAGAAGGA+TGG - chr7_3:3597664-3597683 None:intergenic 40.0%
ATCCTTCTTGTCTTCGTTTG+TGG + chr7_3:3597663-3597682 Msa1089990:CDS 40.0%
TACCACAAACGAAGACAAGA+AGG - chr7_3:3597668-3597687 None:intergenic 40.0%
CTCGAGTTTATGTGTGTGTT+TGG + chr7_3:3597873-3597892 Msa1089990:intron 40.0%
AGCTAGGATTTGTAGCTTCA+TGG + chr7_3:3598198-3598217 Msa1089990:intron 40.0%
! GCTTCATGGAGAATTGCTTT+TGG + chr7_3:3598212-3598231 Msa1089990:intron 40.0%
!! TCATGGAGAATTGCTTTTGG+AGG + chr7_3:3598215-3598234 Msa1089990:intron 40.0%
CTAGCCAAAAACAACGTGTT+TGG - chr7_3:3598266-3598285 None:intergenic 40.0%
! GATCCAGGTGCAATTCTTTT+AGG + chr7_3:3599031-3599050 Msa1089990:intron 40.0%
! TACATGACTTGCCCTTATAC+AGG + chr7_3:3599118-3599137 Msa1089990:CDS 40.0%
ACAAACGAAGACAAGAAGGA+TGG - chr7_3:3597664-3597683 None:intergenic 40.0%
ATCCTTCTTGTCTTCGTTTG+TGG + chr7_3:3597663-3597682 Msa1089990:CDS 40.0%
TACCACAAACGAAGACAAGA+AGG - chr7_3:3597668-3597687 None:intergenic 40.0%
CTCGAGTTTATGTGTGTGTT+TGG + chr7_3:3597873-3597892 Msa1089990:intron 40.0%
AGCTAGGATTTGTAGCTTCA+TGG + chr7_3:3598198-3598217 Msa1089990:intron 40.0%
! GCTTCATGGAGAATTGCTTT+TGG + chr7_3:3598212-3598231 Msa1089990:intron 40.0%
!! TCATGGAGAATTGCTTTTGG+AGG + chr7_3:3598215-3598234 Msa1089990:intron 40.0%
CTAGCCAAAAACAACGTGTT+TGG - chr7_3:3598266-3598285 None:intergenic 40.0%
! GATCCAGGTGCAATTCTTTT+AGG + chr7_3:3599031-3599050 Msa1089990:intron 40.0%
! TACATGACTTGCCCTTATAC+AGG + chr7_3:3599118-3599137 Msa1089990:CDS 40.0%
CCTCCTAAAAGAATTGCACC+TGG - chr7_3:3599037-3599056 None:intergenic 45.0%
! CCAGGTGCAATTCTTTTAGG+AGG + chr7_3:3599034-3599053 Msa1089990:intron 45.0%
AACAATTGCATGCTGGATCG+AGG - chr7_3:3599153-3599172 None:intergenic 45.0%
GCAATGCAACAATTGCATGC+TGG - chr7_3:3599160-3599179 None:intergenic 45.0%
CCTCCTAAAAGAATTGCACC+TGG - chr7_3:3599037-3599056 None:intergenic 45.0%
! CCAGGTGCAATTCTTTTAGG+AGG + chr7_3:3599034-3599053 Msa1089990:intron 45.0%
AACAATTGCATGCTGGATCG+AGG - chr7_3:3599153-3599172 None:intergenic 45.0%
GCAATGCAACAATTGCATGC+TGG - chr7_3:3599160-3599179 None:intergenic 45.0%
!!! TATTTTTTTTTTTGAAAAAA+AGG + chr7_3:3598597-3598616 Msa1089990:intron 5.0%
!!! ACTTATTTTTTTTTAAAAAA+AGG + chr7_3:3598649-3598668 Msa1089990:intron 5.0%
!! AACAAAAATAAATAATAAAT+TGG + chr7_3:3598795-3598814 Msa1089990:intron 5.0%
!!! TTCTAAAAATAAAAATAAAT+TGG + chr7_3:3598973-3598992 Msa1089990:intron 5.0%
!! TAATATTATTTACATAATTA+TGG + chr7_3:3599303-3599322 Msa1089990:three_prime_UTR 5.0%
!!! TATTTTTTTTTTTGAAAAAA+AGG + chr7_3:3598597-3598616 Msa1089990:intron 5.0%
!!! ACTTATTTTTTTTTAAAAAA+AGG + chr7_3:3598649-3598668 Msa1089990:intron 5.0%
!! AACAAAAATAAATAATAAAT+TGG + chr7_3:3598795-3598814 Msa1089990:intron 5.0%
!!! TTCTAAAAATAAAAATAAAT+TGG + chr7_3:3598973-3598992 Msa1089990:intron 5.0%
!! TAATATTATTTACATAATTA+TGG + chr7_3:3599303-3599322 Msa1089990:three_prime_UTR 5.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 3597642 3599470 3597642 ID=Msa1089990;Name=Msa1089990
chr7_3 mRNA 3597642 3599470 3597642 ID=Msa1089990-mRNA-1;Parent=Msa1089990;Name=Msa1089990-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0.5|1|1|1|2|233|80
chr7_3 exon 3597642 3597684 3597642 ID=Msa1089990-mRNA-1:exon:1426;Parent=Msa1089990-mRNA-1
chr7_3 exon 3599038 3599470 3599038 ID=Msa1089990-mRNA-1:exon:1427;Parent=Msa1089990-mRNA-1
chr7_3 CDS 3597642 3597684 3597642 ID=Msa1089990-mRNA-1:cds;Parent=Msa1089990-mRNA-1
chr7_3 CDS 3599038 3599237 3599038 ID=Msa1089990-mRNA-1:cds;Parent=Msa1089990-mRNA-1
chr7_3 three_prime_UTR 3599238 3599470 3599238 ID=Msa1089990-mRNA-1:three_prime_utr;Parent=Msa1089990-mRNA-1
Gene Sequence

>Msa1089990

ATGGCTTTAAAGGTTGGAACCATCCTTCTTGTCTTCGTTTGTGGTGCAATTCTTTTAGGAGGCAATTTGAAAACTGTTGATGCACAAAAAATTTGCCCTCAGTTTTGCTATGATTCAGTTGCATACATGACTTGCCCTTATACAGGTGATAAACACCTCGATCCAGCATGCAATTGTTGCATTGCATCAACTGGATGTGTACTTTATGAAGCAGATGGAACTCGTATTTGTACTGCTGATTAA

Protein sequence

>Msa1089990

MALKVGTILLVFVCGAILLGGNLKTVDAQKICPQFCYDSVAYMTCPYTGDKHLDPACNCCIASTGCVLYEADGTRICTAD*