AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0529110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0529110 MtrunA17_Chr4g0014461 87.500 80 10 0 1 80 1 80 1.64e-47 145
Msa0529110 MtrunA17_Chr4g0014431 81.013 79 15 0 1 79 1 79 5.32e-42 131
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTAATTATGTTGTAATA+TGG 0.214329 4_1:-2444342 Msa0529110:three_prime_UTR
TAATATTATTTACATAATTA+TGG 0.219522 4_1:-2444369 Msa0529110:three_prime_UTR
TGTACTGCTGATTGAAAATA+TGG 0.233285 4_1:-2444449 Msa0529110:exon
ACTCCTATTTGTATGTAATA+TGG 0.281587 4_1:-2444318 Msa0529110:three_prime_UTR
AGGTGTTTATCACCTGTATA+AGG 0.302748 4_1:+2444542 None:intergenic
ATTTGTATGTAATATGGATA+TGG 0.337532 4_1:-2444312 Msa0529110:three_prime_UTR
ATCCTTCTTGTCTTCGTTTG+TGG 0.338806 4_1:-2447705 Msa0529110:intron
TATCCATATTACATACAAAT+AGG 0.368185 4_1:+2444315 None:intergenic
ATGTAATATGGATATGGATA+TGG 0.371160 4_1:-2444306 Msa0529110:three_prime_UTR
AGCAAAATGGCTTTAAAGGT+TGG 0.418570 4_1:-2447732 None:intergenic
AATTGTTGCATTGCATCAAC+TGG 0.473599 4_1:-2444506 Msa0529110:CDS
TACATGACTTGCCCTTATAC+AGG 0.473979 4_1:-2444554 Msa0529110:CDS
GGTGTTTATCACCTGTATAA+GGG 0.520757 4_1:+2444543 None:intergenic
TACCACAAACGAAGACAAGA+AGG 0.582604 4_1:+2447703 None:intergenic
GCAATGCAACAATTGCATGC+TGG 0.616125 4_1:+2444515 None:intergenic
TGTGTACTTTATGAAGCAGA+TGG 0.632102 4_1:-2444482 Msa0529110:CDS
AATATGGATATGGATATGGA+TGG 0.636950 4_1:-2444302 Msa0529110:three_prime_UTR
CTGAATCATAGCAAAACTGA+GGG 0.651830 4_1:+2444582 None:intergenic
ACAAACGAAGACAAGAAGGA+TGG 0.658438 4_1:+2447707 None:intergenic
ACTGAATCATAGCAAAACTG+AGG 0.661702 4_1:+2444581 None:intergenic
AACAATTGCATGCTGGATCG+AGG 0.699789 4_1:+2444522 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTTTAAAAAAATA+CGG + chr4_1:2446374-2446393 None:intergenic 0.0%
!!! ATTTTTTTATTACATAATTG+GGG + chr4_1:2445342-2445361 None:intergenic 10.0%
!!! TTATTAATTTTTCATTTTTC+CGG + chr4_1:2446550-2446569 None:intergenic 10.0%
!! AAGAAAAAATTTAAAAATTC+TGG - chr4_1:2445557-2445576 Msa0529110:intron 10.0%
!! TTTGAAAAAAATTAAAATTC+TGG - chr4_1:2445704-2445723 Msa0529110:intron 10.0%
!! TTTATTTCCAAAAAAAATTT+CGG - chr4_1:2445809-2445828 Msa0529110:intron 10.0%
!! TTAATGAAAAATAAAAATTC+TGG + chr4_1:2446135-2446154 None:intergenic 10.0%
!!! TTAAATTTTAAGAAAAATTC+TGG + chr4_1:2446177-2446196 None:intergenic 10.0%
!! CTAAAAAAAATTAAAATTCT+GGG + chr4_1:2446204-2446223 None:intergenic 10.0%
!! TCTAAAAAAAATTAAAATTC+TGG + chr4_1:2446205-2446224 None:intergenic 10.0%
!!! CAAAATATTTTTATAGTTTT+TGG + chr4_1:2446741-2446760 None:intergenic 10.0%
!!! ATTTTTTTTTGGCTTAAATA+TGG - chr4_1:2445181-2445200 Msa0529110:intron 15.0%
!!! CAAATTTTTTTTATAGGTTT+TGG - chr4_1:2445247-2445266 Msa0529110:intron 15.0%
!! TTATTTCCAAAAAAAATTTC+GGG - chr4_1:2445810-2445829 Msa0529110:intron 15.0%
!!! ATTTTTGTGTATTTATAAAC+CGG - chr4_1:2445951-2445970 Msa0529110:intron 15.0%
!! TATTTATAAATCGAGAATAT+GGG - chr4_1:2445985-2446004 Msa0529110:intron 15.0%
!!! ACTATAAAAAAAAATTTTGC+AGG - chr4_1:2446681-2446700 Msa0529110:intron 15.0%
!!! CTATAAAAAAAAATTTTGCA+GGG - chr4_1:2446682-2446701 Msa0529110:intron 15.0%
!!! AAACTATAAAAATATTTTGC+AGG - chr4_1:2446742-2446761 Msa0529110:intron 15.0%
!!! AACTATAAAAATATTTTGCA+GGG - chr4_1:2446743-2446762 Msa0529110:intron 15.0%
!!! CAAATTTTTTTTATAGGTTT+TGG + chr4_1:2445247-2445266 None:intergenic 15.0%
!! AAAAAAAAAAGGCTTAAATA+TGG + chr4_1:2446868-2446887 None:intergenic 15.0%
!!! CCTTTTTTTCAAAAAAAAAA+AGG + chr4_1:2446879-2446898 None:intergenic 15.0%
!!! CCTTTTTTTTTTTGAAAAAA+AGG - chr4_1:2446876-2446895 Msa0529110:intron 15.0%
!!! ATTTTTTTGTTGAATTGTAT+AGG + chr4_1:2447173-2447192 None:intergenic 15.0%
!! GAATTAATTATGTTGTAATA+TGG - chr4_1:2447608-2447627 Msa0529110:intron 15.0%
!!! TTGTTTCTTTTAGCAATTTA+TGG - chr4_1:2444309-2444328 Msa0529110:three_prime_UTR 20.0%
!!! TAAACAAACTGATTTATGTT+TGG - chr4_1:2444591-2444610 Msa0529110:CDS 20.0%
!! TAAACAAACTTTAACTAAAC+CGG - chr4_1:2445007-2445026 Msa0529110:intron 20.0%
!! ACCTATAAAAAAAATTTGCA+GGG + chr4_1:2446804-2446823 None:intergenic 20.0%
!! AACCTATAAAAAAAATTTGC+AGG + chr4_1:2446803-2446822 None:intergenic 20.0%
!!! CGAAGTTTTAATAAAAATTC+AGG - chr4_1:2445741-2445760 Msa0529110:intron 20.0%
!!! AAAATTCCCGAAATTTTTTT+TGG + chr4_1:2445819-2445838 None:intergenic 20.0%
!! TATTTATAAACCGAGAATAA+GGG - chr4_1:2445842-2445861 Msa0529110:intron 20.0%
!!! TTTTTGTGTATTTATAAACC+GGG - chr4_1:2445952-2445971 Msa0529110:intron 20.0%
!! GTATTTATAAATCGAGAATA+TGG - chr4_1:2445984-2446003 Msa0529110:intron 20.0%
!! CGGAAAAAAATTAAAAATTC+TGG + chr4_1:2446354-2446373 None:intergenic 20.0%
!! AACCTATAAAAAAAATTTGC+AGG - chr4_1:2446803-2446822 Msa0529110:intron 20.0%
!! ACCTATAAAAAAAATTTGCA+GGG - chr4_1:2446804-2446823 Msa0529110:intron 20.0%
!! TATCCATATTACATACAAAT+AGG + chr4_1:2447638-2447657 None:intergenic 20.0%
!! ATTTGTATGTAATATGGATA+TGG - chr4_1:2447638-2447657 Msa0529110:intron 20.0%
!! TGAGAGAATTGATTTATGTT+TGG - chr4_1:2444547-2444566 Msa0529110:CDS 25.0%
! ACTAAAATAGACATAGCTAA+AGG - chr4_1:2444650-2444669 Msa0529110:intron 25.0%
! TAAAGGAGAATTGATAAAGA+AGG - chr4_1:2444667-2444686 Msa0529110:intron 25.0%
! TAAAGAAGGTGAATTGATAA+AGG - chr4_1:2444681-2444700 Msa0529110:intron 25.0%
! AAAGAAGGTGAATTGATAAA+GGG - chr4_1:2444682-2444701 Msa0529110:intron 25.0%
! GGGCAATAAAGAAAAATAAT+AGG - chr4_1:2444702-2444721 Msa0529110:intron 25.0%
!!! TTTTTAAGAGGACTACAAAT+TGG - chr4_1:2444946-2444965 Msa0529110:intron 25.0%
! CTTAATAATTAGACCTTGTA+AGG + chr4_1:2445060-2445079 None:intergenic 25.0%
!!! TTTTGGCTTAAATATGGTAT+TGG - chr4_1:2445187-2445206 Msa0529110:intron 25.0%
!!! TCCCTGCAAATTTTTTTTAT+AGG - chr4_1:2445241-2445260 Msa0529110:intron 25.0%
!! CCAAAAACAAAAATTTTGCA+GGG + chr4_1:2445306-2445325 None:intergenic 25.0%
!! TCCAAAAACAAAAATTTTGC+AGG + chr4_1:2445307-2445326 None:intergenic 25.0%
! GTATTTATAAACCGAGAATA+AGG - chr4_1:2445841-2445860 Msa0529110:intron 25.0%
!!! CATATTTTTTCCCTTATTCT+CGG + chr4_1:2445855-2445874 None:intergenic 25.0%
!! CGAAATTTCGAAATTTTCAT+TGG - chr4_1:2445914-2445933 Msa0529110:intron 25.0%
! TATAAATACACAAAAGTTCC+CGG + chr4_1:2445973-2445992 None:intergenic 25.0%
!!! TCCCTGCAAATTTTTTTTAT+AGG + chr4_1:2445241-2445260 None:intergenic 25.0%
! AAAAGGCTTAAATATGGTAT+TGG + chr4_1:2446862-2446881 None:intergenic 25.0%
! AACTTCATACCTAATTAAGT+TGG - chr4_1:2447014-2447033 Msa0529110:intron 25.0%
!!! TTATTTTTGTTGCAGTACAA+GGG + chr4_1:2447065-2447084 None:intergenic 25.0%
!!! TTTATTTTTGTTGCAGTACA+AGG + chr4_1:2447066-2447085 None:intergenic 25.0%
! ACTCCTATTTGTATGTAATA+TGG - chr4_1:2447632-2447651 Msa0529110:intron 25.0%
! ATGTAATATGGATATGGATA+TGG - chr4_1:2447644-2447663 Msa0529110:intron 25.0%
CAATATGTAGAAAGAAAGAG+AGG + chr4_1:2444286-2444305 None:intergenic 30.0%
!!! TTTTTGGCTAGAATTGCTTT+TGG - chr4_1:2444857-2444876 Msa0529110:intron 30.0%
! ACCATACTTTATTTGAGATG+TGG - chr4_1:2444917-2444936 Msa0529110:intron 30.0%
ACCACATCTCAAATAAAGTA+TGG + chr4_1:2444921-2444940 None:intergenic 30.0%
!!! ATGTGGTGAACTTTTTTAAG+AGG - chr4_1:2444934-2444953 Msa0529110:intron 30.0%
TATTTAGAAACTAAGTGCTC+CGG + chr4_1:2445029-2445048 None:intergenic 30.0%
AAATAACAATCCTCCTTACA+AGG - chr4_1:2445044-2445063 Msa0529110:intron 30.0%
AATAATTAGACCTTGTAAGG+AGG + chr4_1:2445057-2445076 None:intergenic 30.0%
! AACTTAAACAACTTTTTGCG+TGG - chr4_1:2445125-2445144 Msa0529110:intron 30.0%
!!! CCCTGCAAAATTTTTGTTTT+TGG - chr4_1:2445303-2445322 Msa0529110:intron 30.0%
TTATTACATAATTGGGGTCA+GGG + chr4_1:2445336-2445355 None:intergenic 30.0%
TTTATTACATAATTGGGGTC+AGG + chr4_1:2445337-2445356 None:intergenic 30.0%
TGTATTTATAAACCGAGCTT+AGG - chr4_1:2445875-2445894 Msa0529110:intron 30.0%
ATACACAAAAAACCTAAGCT+CGG + chr4_1:2445890-2445909 None:intergenic 30.0%
! ATTTTCCCTGAATTTTTGCA+GGG - chr4_1:2446651-2446670 Msa0529110:intron 30.0%
!!! AAAATTGGCCTATTTTTGCA+GGG - chr4_1:2446714-2446733 Msa0529110:intron 30.0%
!!! AAAACTGACCTATTTTTGCA+GGG - chr4_1:2446775-2446794 Msa0529110:intron 30.0%
CATAAACAGAAACTCAAGAT+AGG - chr4_1:2446981-2447000 Msa0529110:intron 30.0%
CAATTGTGTCCAACTTAATT+AGG + chr4_1:2447026-2447045 None:intergenic 30.0%
!! CTAATTAAGTTGGACACAAT+TGG - chr4_1:2447024-2447043 Msa0529110:intron 30.0%
! AACTTTCTTGTTTATGATCC+AGG - chr4_1:2447294-2447313 Msa0529110:intron 30.0%
! TGTACTGCTGATTGAAAATA+TGG - chr4_1:2447501-2447520 Msa0529110:intron 30.0%
AATATGGATATGGATATGGA+TGG - chr4_1:2447648-2447667 Msa0529110:intron 30.0%
! TTCTAGAAAAGCTAGAAGCT+AGG - chr4_1:2444764-2444783 Msa0529110:intron 35.0%
CAAAAACAACGTGTTTGGTT+TGG + chr4_1:2444843-2444862 None:intergenic 35.0%
!! CAAACCAAACACGTTGTTTT+TGG - chr4_1:2444841-2444860 Msa0529110:intron 35.0%
! GCACTGTAGAGAATTGTTTT+TGG - chr4_1:2444879-2444898 Msa0529110:intron 35.0%
!!! TGTAGAGAATTGTTTTTGGC+TGG - chr4_1:2444883-2444902 Msa0529110:intron 35.0%
!!! GTAGAGAATTGTTTTTGGCT+GGG - chr4_1:2444884-2444903 Msa0529110:intron 35.0%
!!! AAAACTGGCCTATTTTTGCA+GGG + chr4_1:2445274-2445293 None:intergenic 35.0%
! CATTTTCCCTGAATTTTTGC+AGG - chr4_1:2446650-2446669 Msa0529110:intron 35.0%
TTGATCCCTGCAAAAATTCA+GGG + chr4_1:2446659-2446678 None:intergenic 35.0%
TTTGATCCCTGCAAAAATTC+AGG + chr4_1:2446660-2446679 None:intergenic 35.0%
! CTGCAAAAATAGGCCAATTT+TGG + chr4_1:2446715-2446734 None:intergenic 35.0%
!!! CAAAATTGGCCTATTTTTGC+AGG - chr4_1:2446713-2446732 Msa0529110:intron 35.0%
!! TTTTTGGTCCCTGCAAAAAT+AGG + chr4_1:2446725-2446744 None:intergenic 35.0%
! CTGCAAAAATAGGTCAGTTT+TGG + chr4_1:2446776-2446795 None:intergenic 35.0%
!!! CAAAACTGACCTATTTTTGC+AGG - chr4_1:2446774-2446793 Msa0529110:intron 35.0%
GTTTCGTCTTCAGCTTAATT+AGG + chr4_1:2447151-2447170 None:intergenic 35.0%
CTGAATCATAGCAAAACTGA+GGG + chr4_1:2447371-2447390 None:intergenic 35.0%
ACTGAATCATAGCAAAACTG+AGG + chr4_1:2447372-2447391 None:intergenic 35.0%
GGTGTTTATCACCTGTATAA+GGG + chr4_1:2447410-2447429 None:intergenic 35.0%
AGGTGTTTATCACCTGTATA+AGG + chr4_1:2447411-2447430 None:intergenic 35.0%
AATTGTTGCATTGCATCAAC+TGG - chr4_1:2447444-2447463 Msa0529110:intron 35.0%
TGTGTACTTTATGAAGCAGA+TGG - chr4_1:2447468-2447487 Msa0529110:intron 35.0%
ACAAACGAAGACAAGAAGGA+TGG + chr4_1:2444246-2444265 None:intergenic 40.0%
ATCCTTCTTGTCTTCGTTTG+TGG - chr4_1:2444245-2444264 Msa0529110:three_prime_UTR 40.0%
TACCACAAACGAAGACAAGA+AGG + chr4_1:2444250-2444269 None:intergenic 40.0%
CTCGAGTTTATGTGTGTGTT+TGG - chr4_1:2444455-2444474 Msa0529110:exon 40.0%
AGCTAGGATTTGTAGCTTCA+TGG - chr4_1:2444780-2444799 Msa0529110:intron 40.0%
! GCTTCATGGAGAATTGCTTT+TGG - chr4_1:2444794-2444813 Msa0529110:intron 40.0%
!! TCATGGAGAATTGCTTTTGG+AGG - chr4_1:2444797-2444816 Msa0529110:intron 40.0%
CTAGCCAAAAACAACGTGTT+TGG + chr4_1:2444848-2444867 None:intergenic 40.0%
!! CAAAACTGGCCTATTTTGCA+GGG + chr4_1:2446835-2446854 None:intergenic 40.0%
!! GTTTTGGTCCCTGCAAAAAT+AGG - chr4_1:2445263-2445282 Msa0529110:intron 40.0%
!!! CAAAACTGGCCTATTTTTGC+AGG + chr4_1:2445275-2445294 None:intergenic 40.0%
! CTGCAAAAATAGGCCAGTTT+TGG - chr4_1:2445273-2445292 Msa0529110:intron 40.0%
GCAGGGACTAAAACCAAAAT+TGG - chr4_1:2446699-2446718 Msa0529110:intron 40.0%
!! GTTTTGGTCCCTGCAAAAAT+AGG + chr4_1:2445263-2445282 None:intergenic 40.0%
!! CAAAACTGGCCTATTTTGCA+GGG - chr4_1:2446835-2446854 Msa0529110:intron 40.0%
! GATCCAGGTGCAATTCTTTT+AGG - chr4_1:2447309-2447328 Msa0529110:intron 40.0%
! TACATGACTTGCCCTTATAC+AGG - chr4_1:2447396-2447415 Msa0529110:intron 40.0%
!! GGTATTGGTCCCTGCAAAAT+AGG - chr4_1:2445202-2445221 Msa0529110:intron 45.0%
!! CCAAAACTGGCCTATTTTGC+AGG + chr4_1:2446834-2446853 None:intergenic 45.0%
! CCTGCAAAATAGGCCAGTTT+TGG - chr4_1:2445212-2445231 Msa0529110:intron 45.0%
GCAGGGACTAAAACCAAAAC+TGG + chr4_1:2446760-2446779 None:intergenic 45.0%
GCAGGGACTAAAACCAAAAC+TGG + chr4_1:2446760-2446779 None:intergenic 45.0%
GCAGGGACTAAAACCAAAAC+TGG - chr4_1:2446760-2446779 Msa0529110:intron 45.0%
! CCTGCAAAATAGGCCAGTTT+TGG + chr4_1:2445212-2445231 None:intergenic 45.0%
!! CCAAAACTGGCCTATTTTGC+AGG - chr4_1:2446834-2446853 Msa0529110:intron 45.0%
!! GGTATTGGTCCCTGCAAAAT+AGG + chr4_1:2445202-2445221 None:intergenic 45.0%
CCTCCTAAAAGAATTGCACC+TGG + chr4_1:2447315-2447334 None:intergenic 45.0%
! CCAGGTGCAATTCTTTTAGG+AGG - chr4_1:2447312-2447331 Msa0529110:intron 45.0%
AACAATTGCATGCTGGATCG+AGG + chr4_1:2447431-2447450 None:intergenic 45.0%
GCAATGCAACAATTGCATGC+TGG + chr4_1:2447438-2447457 None:intergenic 45.0%
!!! TAAATATACTTATTTTTTTT+TGG - chr4_1:2445170-2445189 Msa0529110:intron 5.0%
!!! AATTTTTTTATTACATAATT+GGG + chr4_1:2445343-2445362 None:intergenic 5.0%
!!! TAATTTTTTTATTACATAAT+TGG + chr4_1:2445344-2445363 None:intergenic 5.0%
!! AAAAAAATATTAAAAAATTC+TGG - chr4_1:2445364-2445383 Msa0529110:intron 5.0%
!! AAAAAATATTAAAAAAATTC+TGG - chr4_1:2445414-2445433 Msa0529110:intron 5.0%
!! TAAAAAATATTAAAAAATTC+CGG - chr4_1:2445451-2445470 Msa0529110:intron 5.0%
!!! AAAAAGTTATTAAAAAATTT+TGG - chr4_1:2445501-2445520 Msa0529110:intron 5.0%
!! AAAAAAATATTAAAAAATTC+TGG + chr4_1:2445364-2445383 None:intergenic 5.0%
!! AAAAAAATATTAAAAAATTC+TGG + chr4_1:2445364-2445383 None:intergenic 5.0%
!! AAAAAAATATTTAAAAATTC+TGG + chr4_1:2446521-2446540 None:intergenic 5.0%
!! ATAAAAAAATTAAAAAATTC+TGG + chr4_1:2446572-2446591 None:intergenic 5.0%
!! AAAAAAAAATTAAAAAATTC+TGG + chr4_1:2446610-2446629 None:intergenic 5.0%
!!! ACTTATTTTTTTTTAAAAAA+AGG - chr4_1:2446928-2446947 Msa0529110:intron 5.0%
!! AACAAAAATAAATAATAAAT+TGG - chr4_1:2447074-2447093 Msa0529110:intron 5.0%
!!! TTCTAAAAATAAAAATAAAT+TGG - chr4_1:2447251-2447270 Msa0529110:intron 5.0%
!! TAATATTATTTACATAATTA+TGG - chr4_1:2447581-2447600 Msa0529110:intron 5.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 2444224 2447748 2444224 ID=Msa0529110;Name=Msa0529110
chr4_1 mRNA 2444224 2447748 2444224 ID=Msa0529110-mRNA-1;Parent=Msa0529110;Name=Msa0529110-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0.5|1|1|1|2|233|80
chr4_1 exon 2444224 2444656 2444224 ID=Msa0529110-mRNA-1:exon:672;Parent=Msa0529110-mRNA-1
chr4_1 exon 2447706 2447748 2447706 ID=Msa0529110-mRNA-1:exon:671;Parent=Msa0529110-mRNA-1
chr4_1 CDS 2447706 2447748 2447706 ID=Msa0529110-mRNA-1:cds;Parent=Msa0529110-mRNA-1
chr4_1 CDS 2444457 2444656 2444457 ID=Msa0529110-mRNA-1:cds;Parent=Msa0529110-mRNA-1
chr4_1 three_prime_UTR 2444224 2444456 2444224 ID=Msa0529110-mRNA-1:three_prime_utr;Parent=Msa0529110-mRNA-1
Gene Sequence

>Msa0529110

ATGGCTTTAAAGGTTGGAACCATCCTTCTTGTCTTCGTTTGTGGTGCAATTCTTTTAGGAGGCAATTTGAAAACTGTTGATGCACAAAAAATTTGCCCTCAGTTTTGCTATGATTCAGTTGCATACATGACTTGCCCTTATACAGGTGATAAACACCTCGATCCAGCATGCAATTGTTGCATTGCATCAACTGGATGTGTACTTTATGAAGCAGATGGAACTCGTATTTGTACTGCTGATTGA

Protein sequence

>Msa0529110

MALKVGTILLVFVCGAILLGGNLKTVDAQKICPQFCYDSVAYMTCPYTGDKHLDPACNCCIASTGCVLYEADGTRICTAD*