AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0235000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235000 MtrunA17_Chr2g0283451 97.500 80 2 0 1 80 1 80 9.19e-50 151
Msa0235000 MtrunA17_Chr2g0325441 73.684 76 20 0 5 80 2 77 5.02e-35 114
Msa0235000 MtrunA17_Chr4g0040991 75.000 76 19 0 5 80 2 77 7.62e-35 113
Msa0235000 MtrunA17_Chr2g0324911 60.811 74 29 0 7 80 5 78 6.00e-21 78.6
Msa0235000 MtrunA17_Chr4g0040491 58.333 72 30 0 9 80 8 79 9.72e-15 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235000 AT2G31090.1 73.684 76 18 1 5 80 2 75 5.25e-27 94.0
Msa0235000 AT2G20562.1 59.420 69 28 0 12 80 5 73 7.65e-20 75.9

Find 26 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTAGGTGCAGTTATT+TGG 0.227999 2_2:+7742686 Msa0235000:CDS
TCTCTCATAATCTCCTTTGT+AGG 0.287883 2_2:+7742673 Msa0235000:CDS
AACCACTCCATAACCTTAAC+TGG 0.322726 2_2:-7742910 None:intergenic
CACATGGTTAATTACCTAAT+GGG 0.332162 2_2:-7742580 None:intergenic
ACACATGGTTAATTACCTAA+TGG 0.347508 2_2:-7742581 None:intergenic
TTTAAGAACTTCAACCCATT+AGG 0.359993 2_2:+7742566 None:intergenic
GGTGCAGTTATTTGGATTGT+TGG 0.388294 2_2:+7742694 Msa0235000:CDS
GTGCAGTTATTTGGATTGTT+GGG 0.397665 2_2:+7742695 Msa0235000:CDS
CAAGGCTCCAGTTAAGGTTA+TGG 0.418089 2_2:+7742903 Msa0235000:CDS
GTTTGCTCTGGCTCTCATCA+AGG 0.420977 2_2:+7742885 Msa0235000:CDS
CCTCATAGTTGAGTTTGCTC+TGG 0.438328 2_2:+7742873 Msa0235000:CDS
TCTCATCAAGGCTCCAGTTA+AGG 0.440222 2_2:+7742897 Msa0235000:CDS
GATTGTAAATTCAGGCCTCT+TGG 0.461509 2_2:+7742619 Msa0235000:CDS
ATGAGAGACAAGAAAGCAAA+AGG 0.478563 2_2:-7742658 None:intergenic
AGAAAGCAAAAGGAAGGCCT+AGG 0.499755 2_2:-7742648 None:intergenic
TTAACCATGTGTTGCTGTTG+TGG 0.511293 2_2:+7742592 Msa0235000:exon
CCAGAGCAAACTCAACTATG+AGG 0.515083 2_2:-7742873 None:intergenic
GTGGTGAGGATTGTAAATTC+AGG 0.531054 2_2:+7742611 Msa0235000:CDS
CCAAATAACTGCACCTACAA+AGG 0.535186 2_2:-7742686 None:intergenic
CATGTGTTGCTGTTGTGGTG+AGG 0.609312 2_2:+7742597 Msa0235000:exon
GAGACAAGAAAGCAAAAGGA+AGG 0.614663 2_2:-7742654 None:intergenic
AGGGTCACGCAAAGACAGCA+TGG 0.623057 2_2:-7742853 None:intergenic
CAGAGCAAACTCAACTATGA+GGG 0.623831 2_2:-7742872 None:intergenic
CTCACCACAACAGCAACACA+TGG 0.630485 2_2:-7742596 None:intergenic
CTCCAGTTAAGGTTATGGAG+TGG 0.661349 2_2:+7742908 Msa0235000:CDS
AGGCCTAGGAGAAAACCAAG+AGG 0.747286 2_2:-7742634 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAAATACTAATAGAT+TGG - chr2_2:7742737-7742756 None:intergenic 15.0%
! AATGTGTATGATGCTATATA+TGG + chr2_2:7742805-7742824 Msa0235000:intron 25.0%
CACATGGTTAATTACCTAAT+GGG - chr2_2:7742583-7742602 None:intergenic 30.0%
ACACATGGTTAATTACCTAA+TGG - chr2_2:7742584-7742603 None:intergenic 30.0%
CTAATAGATTGGAAAAGAGA+GGG - chr2_2:7742726-7742745 None:intergenic 30.0%
ACTAATAGATTGGAAAAGAG+AGG - chr2_2:7742727-7742746 None:intergenic 30.0%
ATGAGAGACAAGAAAGCAAA+AGG - chr2_2:7742661-7742680 None:intergenic 35.0%
TCTCTCATAATCTCCTTTGT+AGG + chr2_2:7742673-7742692 Msa0235000:CDS 35.0%
GTGCAGTTATTTGGATTGTT+GGG + chr2_2:7742695-7742714 Msa0235000:CDS 35.0%
TGTATGATGCTATATATGGC+AGG + chr2_2:7742809-7742828 Msa0235000:intron 35.0%
! TTAACCATGTGTTGCTGTTG+TGG + chr2_2:7742592-7742611 Msa0235000:exon 40.0%
GTGGTGAGGATTGTAAATTC+AGG + chr2_2:7742611-7742630 Msa0235000:CDS 40.0%
GATTGTAAATTCAGGCCTCT+TGG + chr2_2:7742619-7742638 Msa0235000:CDS 40.0%
GAGACAAGAAAGCAAAAGGA+AGG - chr2_2:7742657-7742676 None:intergenic 40.0%
CCAAATAACTGCACCTACAA+AGG - chr2_2:7742689-7742708 None:intergenic 40.0%
CCTTTGTAGGTGCAGTTATT+TGG + chr2_2:7742686-7742705 Msa0235000:CDS 40.0%
GGTGCAGTTATTTGGATTGT+TGG + chr2_2:7742694-7742713 Msa0235000:CDS 40.0%
CAGAGCAAACTCAACTATGA+GGG - chr2_2:7742875-7742894 None:intergenic 40.0%
AACCACTCCATAACCTTAAC+TGG - chr2_2:7742913-7742932 None:intergenic 40.0%
AGAAAGCAAAAGGAAGGCCT+AGG - chr2_2:7742651-7742670 None:intergenic 45.0%
CCAGAGCAAACTCAACTATG+AGG - chr2_2:7742876-7742895 None:intergenic 45.0%
! CCTCATAGTTGAGTTTGCTC+TGG + chr2_2:7742873-7742892 Msa0235000:CDS 45.0%
TCTCATCAAGGCTCCAGTTA+AGG + chr2_2:7742897-7742916 Msa0235000:CDS 45.0%
CAAGGCTCCAGTTAAGGTTA+TGG + chr2_2:7742903-7742922 Msa0235000:CDS 45.0%
CTCCAGTTAAGGTTATGGAG+TGG + chr2_2:7742908-7742927 Msa0235000:CDS 45.0%
CTCACCACAACAGCAACACA+TGG - chr2_2:7742599-7742618 None:intergenic 50.0%
! CATGTGTTGCTGTTGTGGTG+AGG + chr2_2:7742597-7742616 Msa0235000:exon 50.0%
!! AGGCCTCTTGGTTTTCTCCT+AGG + chr2_2:7742631-7742650 Msa0235000:CDS 50.0%
AGGCCTAGGAGAAAACCAAG+AGG - chr2_2:7742637-7742656 None:intergenic 50.0%
!! GTTTGCTCTGGCTCTCATCA+AGG + chr2_2:7742885-7742904 Msa0235000:CDS 50.0%
AGGGTCACGCAAAGACAGCA+TGG - chr2_2:7742856-7742875 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 7742569 7742954 7742569 ID=Msa0235000;Name=Msa0235000
chr2_2 mRNA 7742569 7742954 7742569 ID=Msa0235000-mRNA-1;Parent=Msa0235000;Name=Msa0235000-mRNA-1;_AED=0.15;_eAED=0.15;_QI=29|1|0.5|1|1|1|2|0|80
chr2_2 exon 7742569 7742716 7742569 ID=Msa0235000-mRNA-1:exon:3378;Parent=Msa0235000-mRNA-1
chr2_2 exon 7742831 7742954 7742831 ID=Msa0235000-mRNA-1:exon:3379;Parent=Msa0235000-mRNA-1
chr2_2 five_prime_UTR 7742569 7742597 7742569 ID=Msa0235000-mRNA-1:five_prime_utr;Parent=Msa0235000-mRNA-1
chr2_2 CDS 7742598 7742716 7742598 ID=Msa0235000-mRNA-1:cds;Parent=Msa0235000-mRNA-1
chr2_2 CDS 7742831 7742954 7742831 ID=Msa0235000-mRNA-1:cds;Parent=Msa0235000-mRNA-1
Gene Sequence

>Msa0235000

ATGTGTTGCTGTTGTGGTGAGGATTGTAAATTCAGGCCTCTTGGTTTTCTCCTAGGCCTTCCTTTTGCTTTCTTGTCTCTCATAATCTCCTTTGTAGGTGCAGTTATTTGGATTGTTGGGTTGACTTTGACATGTATATGTCCATGCTGTCTTTGCGTGACCCTCATAGTTGAGTTTGCTCTGGCTCTCATCAAGGCTCCAGTTAAGGTTATGGAGTGGTTCACATCCAAGATTCCATGTTAA

Protein sequence

>Msa0235000

MCCCCGEDCKFRPLGFLLGLPFAFLSLIISFVGAVIWIVGLTLTCICPCCLCVTLIVEFALALIKAPVKVMEWFTSKIPC*