AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0595270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0595270 MtrunA17_Chr4g0014461 87.500 80 10 0 1 80 1 80 1.64e-47 145
Msa0595270 MtrunA17_Chr4g0014431 81.013 79 15 0 1 79 1 79 5.32e-42 131
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTAATTATGTTGTAATA+TGG 0.214329 4_2:-24234608 Msa0595270:three_prime_UTR
TAATATTATTTACATAATTA+TGG 0.219522 4_2:-24234635 Msa0595270:three_prime_UTR
TGTACTGCTGATTGAAAATA+TGG 0.233285 4_2:-24234715 Msa0595270:exon
ACTCCTATTTGTATGTAATA+TGG 0.281587 4_2:-24234584 Msa0595270:three_prime_UTR
AGGTGTTTATCACCTGTATA+AGG 0.302748 4_2:+24234808 None:intergenic
ATTTGTATGTAATATGGATA+TGG 0.337532 4_2:-24234578 Msa0595270:three_prime_UTR
ATCCTTCTTGTCTTCGTTTG+TGG 0.338806 4_2:-24236275 Msa0595270:intron
TATCCATATTACATACAAAT+AGG 0.368185 4_2:+24234581 None:intergenic
ATGTAATATGGATATGGATA+TGG 0.371160 4_2:-24234572 Msa0595270:three_prime_UTR
AGCAAAATGGCTTTAAAGGT+TGG 0.418570 4_2:-24236302 None:intergenic
AATTGTTGCATTGCATCAAC+TGG 0.473599 4_2:-24234772 Msa0595270:CDS
TACATGACTTGCCCTTATAC+AGG 0.473979 4_2:-24234820 Msa0595270:CDS
GGTGTTTATCACCTGTATAA+GGG 0.520757 4_2:+24234809 None:intergenic
TACCACAAACGAAGACAAGA+AGG 0.582604 4_2:+24236273 None:intergenic
GCAATGCAACAATTGCATGC+TGG 0.616125 4_2:+24234781 None:intergenic
TGTGTACTTTATGAAGCAGA+TGG 0.632102 4_2:-24234748 Msa0595270:CDS
AATATGGATATGGATATGGA+TGG 0.636950 4_2:-24234568 Msa0595270:three_prime_UTR
CTGAATCATAGCAAAACTGA+GGG 0.651830 4_2:+24234848 None:intergenic
ACAAACGAAGACAAGAAGGA+TGG 0.658438 4_2:+24236277 None:intergenic
ACTGAATCATAGCAAAACTG+AGG 0.661702 4_2:+24234847 None:intergenic
AACAATTGCATGCTGGATCG+AGG 0.699789 4_2:+24234788 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTGTTGAATTGTAT+AGG + chr4_2:24235743-24235762 None:intergenic 15.0%
!! GAATTAATTATGTTGTAATA+TGG - chr4_2:24236178-24236197 Msa0595270:intron 15.0%
!!! ATTTTTTTGTTGAATTGTAT+AGG + chr4_2:24235743-24235762 None:intergenic 15.0%
!! GAATTAATTATGTTGTAATA+TGG - chr4_2:24236178-24236197 Msa0595270:intron 15.0%
!!! TTGTTTCTTTTAGCAATTTA+TGG - chr4_2:24234575-24234594 Msa0595270:three_prime_UTR 20.0%
!!! TAAACAAACTGATTTATGTT+TGG - chr4_2:24234857-24234876 Msa0595270:CDS 20.0%
!! TAAACAAACTTTAACTAAAC+CGG - chr4_2:24235273-24235292 Msa0595270:intron 20.0%
!! TATCCATATTACATACAAAT+AGG + chr4_2:24236208-24236227 None:intergenic 20.0%
!! ATTTGTATGTAATATGGATA+TGG - chr4_2:24236208-24236227 Msa0595270:intron 20.0%
!!! TTGTTTCTTTTAGCAATTTA+TGG - chr4_2:24234575-24234594 Msa0595270:three_prime_UTR 20.0%
!!! TAAACAAACTGATTTATGTT+TGG - chr4_2:24234857-24234876 Msa0595270:CDS 20.0%
!! TAAACAAACTTTAACTAAAC+CGG - chr4_2:24235273-24235292 Msa0595270:intron 20.0%
!! TATCCATATTACATACAAAT+AGG + chr4_2:24236208-24236227 None:intergenic 20.0%
!! ATTTGTATGTAATATGGATA+TGG - chr4_2:24236208-24236227 Msa0595270:intron 20.0%
!! TGAGAGAATTGATTTATGTT+TGG - chr4_2:24234813-24234832 Msa0595270:CDS 25.0%
! ACTAAAATAGACATAGCTAA+AGG - chr4_2:24234916-24234935 Msa0595270:intron 25.0%
! TAAAGGAGAATTGATAAAGA+AGG - chr4_2:24234933-24234952 Msa0595270:intron 25.0%
! TAAAGAAGGTGAATTGATAA+AGG - chr4_2:24234947-24234966 Msa0595270:intron 25.0%
! AAAGAAGGTGAATTGATAAA+GGG - chr4_2:24234948-24234967 Msa0595270:intron 25.0%
! GGGCAATAAAGAAAAATAAT+AGG - chr4_2:24234968-24234987 Msa0595270:intron 25.0%
!!! TTTTTAAGAGGACTACAAAT+TGG - chr4_2:24235212-24235231 Msa0595270:intron 25.0%
! CTTAATAATTAGACCTTGTA+AGG + chr4_2:24235326-24235345 None:intergenic 25.0%
! AACTTCATACCTAATTAAGT+TGG - chr4_2:24235583-24235602 Msa0595270:intron 25.0%
!!! TTATTTTTGTTGCAGTACAA+GGG + chr4_2:24235634-24235653 None:intergenic 25.0%
!!! TTTATTTTTGTTGCAGTACA+AGG + chr4_2:24235635-24235654 None:intergenic 25.0%
! ACTCCTATTTGTATGTAATA+TGG - chr4_2:24236202-24236221 Msa0595270:intron 25.0%
! ATGTAATATGGATATGGATA+TGG - chr4_2:24236214-24236233 Msa0595270:intron 25.0%
!! TGAGAGAATTGATTTATGTT+TGG - chr4_2:24234813-24234832 Msa0595270:CDS 25.0%
! ACTAAAATAGACATAGCTAA+AGG - chr4_2:24234916-24234935 Msa0595270:intron 25.0%
! TAAAGGAGAATTGATAAAGA+AGG - chr4_2:24234933-24234952 Msa0595270:intron 25.0%
! TAAAGAAGGTGAATTGATAA+AGG - chr4_2:24234947-24234966 Msa0595270:intron 25.0%
! AAAGAAGGTGAATTGATAAA+GGG - chr4_2:24234948-24234967 Msa0595270:intron 25.0%
! GGGCAATAAAGAAAAATAAT+AGG - chr4_2:24234968-24234987 Msa0595270:intron 25.0%
!!! TTTTTAAGAGGACTACAAAT+TGG - chr4_2:24235212-24235231 Msa0595270:intron 25.0%
! CTTAATAATTAGACCTTGTA+AGG + chr4_2:24235326-24235345 None:intergenic 25.0%
! AACTTCATACCTAATTAAGT+TGG - chr4_2:24235583-24235602 Msa0595270:intron 25.0%
!!! TTATTTTTGTTGCAGTACAA+GGG + chr4_2:24235634-24235653 None:intergenic 25.0%
!!! TTTATTTTTGTTGCAGTACA+AGG + chr4_2:24235635-24235654 None:intergenic 25.0%
! ACTCCTATTTGTATGTAATA+TGG - chr4_2:24236202-24236221 Msa0595270:intron 25.0%
! ATGTAATATGGATATGGATA+TGG - chr4_2:24236214-24236233 Msa0595270:intron 25.0%
CAATATGTAGAAAGAAAGAG+AGG + chr4_2:24234552-24234571 None:intergenic 30.0%
!!! TTTTTGGCTAGAATTGCTTT+TGG - chr4_2:24235123-24235142 Msa0595270:intron 30.0%
! ACCATACTTTATTTGAGATG+TGG - chr4_2:24235183-24235202 Msa0595270:intron 30.0%
ACCACATCTCAAATAAAGTA+TGG + chr4_2:24235187-24235206 None:intergenic 30.0%
!!! ATGTGGTGAACTTTTTTAAG+AGG - chr4_2:24235200-24235219 Msa0595270:intron 30.0%
TATTTAGAAACTAAGTGCTC+CGG + chr4_2:24235295-24235314 None:intergenic 30.0%
AAATAACAATCCTCCTTACA+AGG - chr4_2:24235310-24235329 Msa0595270:intron 30.0%
AATAATTAGACCTTGTAAGG+AGG + chr4_2:24235323-24235342 None:intergenic 30.0%
! AACTTAAACAACTTTTTGCG+TGG - chr4_2:24235390-24235409 Msa0595270:intron 30.0%
CATAAACAGAAACTCAAGAT+AGG - chr4_2:24235550-24235569 Msa0595270:intron 30.0%
CAATTGTGTCCAACTTAATT+AGG + chr4_2:24235595-24235614 None:intergenic 30.0%
!! CTAATTAAGTTGGACACAAT+TGG - chr4_2:24235593-24235612 Msa0595270:intron 30.0%
! AACTTTCTTGTTTATGATCC+AGG - chr4_2:24235864-24235883 Msa0595270:intron 30.0%
! TGTACTGCTGATTGAAAATA+TGG - chr4_2:24236071-24236090 Msa0595270:intron 30.0%
AATATGGATATGGATATGGA+TGG - chr4_2:24236218-24236237 Msa0595270:intron 30.0%
CAATATGTAGAAAGAAAGAG+AGG + chr4_2:24234552-24234571 None:intergenic 30.0%
!!! TTTTTGGCTAGAATTGCTTT+TGG - chr4_2:24235123-24235142 Msa0595270:intron 30.0%
! ACCATACTTTATTTGAGATG+TGG - chr4_2:24235183-24235202 Msa0595270:intron 30.0%
ACCACATCTCAAATAAAGTA+TGG + chr4_2:24235187-24235206 None:intergenic 30.0%
!!! ATGTGGTGAACTTTTTTAAG+AGG - chr4_2:24235200-24235219 Msa0595270:intron 30.0%
TATTTAGAAACTAAGTGCTC+CGG + chr4_2:24235295-24235314 None:intergenic 30.0%
AAATAACAATCCTCCTTACA+AGG - chr4_2:24235310-24235329 Msa0595270:intron 30.0%
AATAATTAGACCTTGTAAGG+AGG + chr4_2:24235323-24235342 None:intergenic 30.0%
! AACTTAAACAACTTTTTGCG+TGG - chr4_2:24235390-24235409 Msa0595270:intron 30.0%
CATAAACAGAAACTCAAGAT+AGG - chr4_2:24235550-24235569 Msa0595270:intron 30.0%
CAATTGTGTCCAACTTAATT+AGG + chr4_2:24235595-24235614 None:intergenic 30.0%
!! CTAATTAAGTTGGACACAAT+TGG - chr4_2:24235593-24235612 Msa0595270:intron 30.0%
! AACTTTCTTGTTTATGATCC+AGG - chr4_2:24235864-24235883 Msa0595270:intron 30.0%
! TGTACTGCTGATTGAAAATA+TGG - chr4_2:24236071-24236090 Msa0595270:intron 30.0%
AATATGGATATGGATATGGA+TGG - chr4_2:24236218-24236237 Msa0595270:intron 30.0%
! TTCTAGAAAAGCTAGAAGCT+AGG - chr4_2:24235030-24235049 Msa0595270:intron 35.0%
CAAAAACAACGTGTTTGGTT+TGG + chr4_2:24235109-24235128 None:intergenic 35.0%
!! CAAACCAAACACGTTGTTTT+TGG - chr4_2:24235107-24235126 Msa0595270:intron 35.0%
! GCACTGTAGAGAATTGTTTT+TGG - chr4_2:24235145-24235164 Msa0595270:intron 35.0%
!!! TGTAGAGAATTGTTTTTGGC+TGG - chr4_2:24235149-24235168 Msa0595270:intron 35.0%
!!! GTAGAGAATTGTTTTTGGCT+GGG - chr4_2:24235150-24235169 Msa0595270:intron 35.0%
GTTTCGTCTTCAGCTTAATT+AGG + chr4_2:24235721-24235740 None:intergenic 35.0%
CTGAATCATAGCAAAACTGA+GGG + chr4_2:24235941-24235960 None:intergenic 35.0%
ACTGAATCATAGCAAAACTG+AGG + chr4_2:24235942-24235961 None:intergenic 35.0%
GGTGTTTATCACCTGTATAA+GGG + chr4_2:24235980-24235999 None:intergenic 35.0%
AGGTGTTTATCACCTGTATA+AGG + chr4_2:24235981-24236000 None:intergenic 35.0%
AATTGTTGCATTGCATCAAC+TGG - chr4_2:24236014-24236033 Msa0595270:intron 35.0%
TGTGTACTTTATGAAGCAGA+TGG - chr4_2:24236038-24236057 Msa0595270:intron 35.0%
! TTCTAGAAAAGCTAGAAGCT+AGG - chr4_2:24235030-24235049 Msa0595270:intron 35.0%
CAAAAACAACGTGTTTGGTT+TGG + chr4_2:24235109-24235128 None:intergenic 35.0%
!! CAAACCAAACACGTTGTTTT+TGG - chr4_2:24235107-24235126 Msa0595270:intron 35.0%
! GCACTGTAGAGAATTGTTTT+TGG - chr4_2:24235145-24235164 Msa0595270:intron 35.0%
!!! TGTAGAGAATTGTTTTTGGC+TGG - chr4_2:24235149-24235168 Msa0595270:intron 35.0%
!!! GTAGAGAATTGTTTTTGGCT+GGG - chr4_2:24235150-24235169 Msa0595270:intron 35.0%
GTTTCGTCTTCAGCTTAATT+AGG + chr4_2:24235721-24235740 None:intergenic 35.0%
CTGAATCATAGCAAAACTGA+GGG + chr4_2:24235941-24235960 None:intergenic 35.0%
ACTGAATCATAGCAAAACTG+AGG + chr4_2:24235942-24235961 None:intergenic 35.0%
GGTGTTTATCACCTGTATAA+GGG + chr4_2:24235980-24235999 None:intergenic 35.0%
AGGTGTTTATCACCTGTATA+AGG + chr4_2:24235981-24236000 None:intergenic 35.0%
AATTGTTGCATTGCATCAAC+TGG - chr4_2:24236014-24236033 Msa0595270:intron 35.0%
TGTGTACTTTATGAAGCAGA+TGG - chr4_2:24236038-24236057 Msa0595270:intron 35.0%
ACAAACGAAGACAAGAAGGA+TGG + chr4_2:24234512-24234531 None:intergenic 40.0%
ATCCTTCTTGTCTTCGTTTG+TGG - chr4_2:24234511-24234530 Msa0595270:three_prime_UTR 40.0%
TACCACAAACGAAGACAAGA+AGG + chr4_2:24234516-24234535 None:intergenic 40.0%
CTCGAGTTTATGTGTGTGTT+TGG - chr4_2:24234721-24234740 Msa0595270:exon 40.0%
AGCTAGGATTTGTAGCTTCA+TGG - chr4_2:24235046-24235065 Msa0595270:intron 40.0%
! GCTTCATGGAGAATTGCTTT+TGG - chr4_2:24235060-24235079 Msa0595270:intron 40.0%
!! TCATGGAGAATTGCTTTTGG+AGG - chr4_2:24235063-24235082 Msa0595270:intron 40.0%
CTAGCCAAAAACAACGTGTT+TGG + chr4_2:24235114-24235133 None:intergenic 40.0%
! GATCCAGGTGCAATTCTTTT+AGG - chr4_2:24235879-24235898 Msa0595270:intron 40.0%
! TACATGACTTGCCCTTATAC+AGG - chr4_2:24235966-24235985 Msa0595270:intron 40.0%
ACAAACGAAGACAAGAAGGA+TGG + chr4_2:24234512-24234531 None:intergenic 40.0%
ATCCTTCTTGTCTTCGTTTG+TGG - chr4_2:24234511-24234530 Msa0595270:three_prime_UTR 40.0%
TACCACAAACGAAGACAAGA+AGG + chr4_2:24234516-24234535 None:intergenic 40.0%
CTCGAGTTTATGTGTGTGTT+TGG - chr4_2:24234721-24234740 Msa0595270:exon 40.0%
AGCTAGGATTTGTAGCTTCA+TGG - chr4_2:24235046-24235065 Msa0595270:intron 40.0%
! GCTTCATGGAGAATTGCTTT+TGG - chr4_2:24235060-24235079 Msa0595270:intron 40.0%
!! TCATGGAGAATTGCTTTTGG+AGG - chr4_2:24235063-24235082 Msa0595270:intron 40.0%
CTAGCCAAAAACAACGTGTT+TGG + chr4_2:24235114-24235133 None:intergenic 40.0%
! GATCCAGGTGCAATTCTTTT+AGG - chr4_2:24235879-24235898 Msa0595270:intron 40.0%
! TACATGACTTGCCCTTATAC+AGG - chr4_2:24235966-24235985 Msa0595270:intron 40.0%
CCTCCTAAAAGAATTGCACC+TGG + chr4_2:24235885-24235904 None:intergenic 45.0%
! CCAGGTGCAATTCTTTTAGG+AGG - chr4_2:24235882-24235901 Msa0595270:intron 45.0%
AACAATTGCATGCTGGATCG+AGG + chr4_2:24236001-24236020 None:intergenic 45.0%
GCAATGCAACAATTGCATGC+TGG + chr4_2:24236008-24236027 None:intergenic 45.0%
CCTCCTAAAAGAATTGCACC+TGG + chr4_2:24235885-24235904 None:intergenic 45.0%
! CCAGGTGCAATTCTTTTAGG+AGG - chr4_2:24235882-24235901 Msa0595270:intron 45.0%
AACAATTGCATGCTGGATCG+AGG + chr4_2:24236001-24236020 None:intergenic 45.0%
GCAATGCAACAATTGCATGC+TGG + chr4_2:24236008-24236027 None:intergenic 45.0%
!!! TATTTTTTTTTTTGAAAAAA+AGG - chr4_2:24235445-24235464 Msa0595270:intron 5.0%
!!! ACTTATTTTTTTTTAAAAAA+AGG - chr4_2:24235497-24235516 Msa0595270:intron 5.0%
!! AACAAAAATAAATAATAAAT+TGG - chr4_2:24235643-24235662 Msa0595270:intron 5.0%
!!! TTCTAAAAATAAAAATAAAT+TGG - chr4_2:24235821-24235840 Msa0595270:intron 5.0%
!! TAATATTATTTACATAATTA+TGG - chr4_2:24236151-24236170 Msa0595270:intron 5.0%
!!! TATTTTTTTTTTTGAAAAAA+AGG - chr4_2:24235445-24235464 Msa0595270:intron 5.0%
!!! ACTTATTTTTTTTTAAAAAA+AGG - chr4_2:24235497-24235516 Msa0595270:intron 5.0%
!! AACAAAAATAAATAATAAAT+TGG - chr4_2:24235643-24235662 Msa0595270:intron 5.0%
!!! TTCTAAAAATAAAAATAAAT+TGG - chr4_2:24235821-24235840 Msa0595270:intron 5.0%
!! TAATATTATTTACATAATTA+TGG - chr4_2:24236151-24236170 Msa0595270:intron 5.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 24234490 24236318 24234490 ID=Msa0595270;Name=Msa0595270
chr4_2 mRNA 24234490 24236318 24234490 ID=Msa0595270-mRNA-1;Parent=Msa0595270;Name=Msa0595270-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0.5|1|1|1|2|233|80
chr4_2 exon 24234490 24234922 24234490 ID=Msa0595270-mRNA-1:exon:6552;Parent=Msa0595270-mRNA-1
chr4_2 exon 24236276 24236318 24236276 ID=Msa0595270-mRNA-1:exon:6551;Parent=Msa0595270-mRNA-1
chr4_2 CDS 24236276 24236318 24236276 ID=Msa0595270-mRNA-1:cds;Parent=Msa0595270-mRNA-1
chr4_2 CDS 24234723 24234922 24234723 ID=Msa0595270-mRNA-1:cds;Parent=Msa0595270-mRNA-1
chr4_2 three_prime_UTR 24234490 24234722 24234490 ID=Msa0595270-mRNA-1:three_prime_utr;Parent=Msa0595270-mRNA-1
Gene Sequence

>Msa0595270

ATGGCTTTAAAGGTTGGAACCATCCTTCTTGTCTTCGTTTGTGGTGCAATTCTTTTAGGAGGCAATTTGAAAACTGTTGATGCACAAAAAATTTGCCCTCAGTTTTGCTATGATTCAGTTGCATACATGACTTGCCCTTATACAGGTGATAAACACCTCGATCCAGCATGCAATTGTTGCATTGCATCAACTGGATGTGTACTTTATGAAGCAGATGGAACTCGTATTTGTACTGCTGATTGA

Protein sequence

>Msa0595270

MALKVGTILLVFVCGAILLGGNLKTVDAQKICPQFCYDSVAYMTCPYTGDKHLDPACNCCIASTGCVLYEADGTRICTAD*