AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0750350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750350 MtrunA17_Chr5g0396061 92.473 93 7 0 12 104 1 93 1.04e-59 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750350 AT2G39725.1 67.391 92 27 1 12 103 1 89 1.73e-38 124
Msa0750350 AT2G39725.2 67.391 92 27 1 12 103 1 89 1.73e-38 124

Find 15 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAGAGTGCAAAGGCTTTC+TGG 0.170483 5_2:-3749746 Msa0750350:CDS
TGAGTGAAAATTAAGTGAAT+TGG 0.300921 5_2:-3749371 Msa0750350:three_prime_UTR
CTACATGAAAAGTATAATAA+TGG 0.323072 5_2:+3749434 None:intergenic
GCGGCGTTCTTCGTCGTGTT+TGG 0.337693 5_2:+3749670 None:intergenic
CTTAACAACCCTGGTACCAC+TGG 0.425728 5_2:-3749533 Msa0750350:CDS
GAAGATAACCCAGTGGTACC+AGG 0.475476 5_2:+3749524 None:intergenic
TCCAGAAAGCCTTTGCACTC+TGG 0.484965 5_2:+3749745 None:intergenic
ATTGAATATTTACTTCGTCG+TGG 0.613668 5_2:-3749575 Msa0750350:CDS
AAGATAACCCAGTGGTACCA+GGG 0.621203 5_2:+3749525 None:intergenic
ATACTAAAGATTCTATGTTG+CGG 0.622444 5_2:+3749651 None:intergenic
TTAACAACCCTGGTACCACT+GGG 0.624488 5_2:-3749532 Msa0750350:CDS
CAAGTGCTTAGCTTATACAG+AGG 0.632036 5_2:-3749713 Msa0750350:CDS
TGGGAGGAACCAGAGTGCAA+AGG 0.649416 5_2:-3749754 Msa0750350:CDS
CTTGATCAGCTTAACAACCC+TGG 0.668759 5_2:-3749542 Msa0750350:CDS
TTGCAATGAAGATAACCCAG+TGG 0.758644 5_2:+3749517 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAGATTTGAACATTTAAT+AGG - chr5_2:3749344-3749363 Msa0750350:three_prime_UTR 20.0%
!!! TGCTCATAATCAAATAATTT+TGG + chr5_2:3749384-3749403 None:intergenic 20.0%
!! TTATGAAATGATAAATGTGA+GGG + chr5_2:3749440-3749459 None:intergenic 20.0%
!! ATTATGAAATGATAAATGTG+AGG + chr5_2:3749441-3749460 None:intergenic 20.0%
!! AGAAAAAAAGAAGATGAAAT+GGG - chr5_2:3749864-3749883 Msa0750350:intron 20.0%
!!! GATCAATTTCTTTTGAATTA+CGG + chr5_2:3750025-3750044 None:intergenic 20.0%
!!! CTACATGAAAAGTATAATAA+TGG + chr5_2:3750206-3750225 None:intergenic 20.0%
!! GATAGATTTGAACATTTAAT+AGG - chr5_2:3749344-3749363 Msa0750350:three_prime_UTR 20.0%
!!! TGCTCATAATCAAATAATTT+TGG + chr5_2:3749384-3749403 None:intergenic 20.0%
!! TTATGAAATGATAAATGTGA+GGG + chr5_2:3749440-3749459 None:intergenic 20.0%
!! ATTATGAAATGATAAATGTG+AGG + chr5_2:3749441-3749460 None:intergenic 20.0%
!! AGAAAAAAAGAAGATGAAAT+GGG - chr5_2:3749864-3749883 Msa0750350:intron 20.0%
!!! GATCAATTTCTTTTGAATTA+CGG + chr5_2:3750025-3750044 None:intergenic 20.0%
!!! CTACATGAAAAGTATAATAA+TGG + chr5_2:3750206-3750225 None:intergenic 20.0%
! TATGAAATGATAAATGTGAG+GGG + chr5_2:3749439-3749458 None:intergenic 25.0%
! TATTGATCGCATAAATCTAT+TGG - chr5_2:3749460-3749479 Msa0750350:three_prime_UTR 25.0%
! ATTGATCGCATAAATCTATT+GGG - chr5_2:3749461-3749480 Msa0750350:three_prime_UTR 25.0%
! CTGATAAAAAAACAATGATC+TGG + chr5_2:3749495-3749514 None:intergenic 25.0%
!! TTAGAACAGAGATCTAAAAT+GGG + chr5_2:3749629-3749648 None:intergenic 25.0%
!!! TAAAATTGATTTTACCTGAG+AGG + chr5_2:3749761-3749780 None:intergenic 25.0%
! AATGTTCTGCGTTTATATTA+TGG - chr5_2:3749838-3749857 Msa0750350:intron 25.0%
! GAGAAAAAAAGAAGATGAAA+TGG - chr5_2:3749863-3749882 Msa0750350:intron 25.0%
! ATACTAAAGATTCTATGTTG+CGG + chr5_2:3749989-3750008 None:intergenic 25.0%
! TGAGTGAAAATTAAGTGAAT+TGG - chr5_2:3750266-3750285 Msa0750350:intron 25.0%
! TATGAAATGATAAATGTGAG+GGG + chr5_2:3749439-3749458 None:intergenic 25.0%
! TATTGATCGCATAAATCTAT+TGG - chr5_2:3749460-3749479 Msa0750350:three_prime_UTR 25.0%
! ATTGATCGCATAAATCTATT+GGG - chr5_2:3749461-3749480 Msa0750350:three_prime_UTR 25.0%
! CTGATAAAAAAACAATGATC+TGG + chr5_2:3749495-3749514 None:intergenic 25.0%
!! TTAGAACAGAGATCTAAAAT+GGG + chr5_2:3749629-3749648 None:intergenic 25.0%
!!! TAAAATTGATTTTACCTGAG+AGG + chr5_2:3749761-3749780 None:intergenic 25.0%
! AATGTTCTGCGTTTATATTA+TGG - chr5_2:3749838-3749857 Msa0750350:intron 25.0%
! GAGAAAAAAAGAAGATGAAA+TGG - chr5_2:3749863-3749882 Msa0750350:intron 25.0%
! ATACTAAAGATTCTATGTTG+CGG + chr5_2:3749989-3750008 None:intergenic 25.0%
! TGAGTGAAAATTAAGTGAAT+TGG - chr5_2:3750266-3750285 Msa0750350:intron 25.0%
! AGATCTAAAATGGGCTTATT+GGG + chr5_2:3749620-3749639 None:intergenic 30.0%
CTTAGAACAGAGATCTAAAA+TGG + chr5_2:3749630-3749649 None:intergenic 30.0%
! GTTTGAGATTTAGATTCGTT+AGG + chr5_2:3749709-3749728 None:intergenic 30.0%
AAAAAAGAAGATGAAATGGG+AGG - chr5_2:3749867-3749886 Msa0750350:intron 30.0%
ATTGAATATTTACTTCGTCG+TGG - chr5_2:3750062-3750081 Msa0750350:intron 30.0%
! AGATCTAAAATGGGCTTATT+GGG + chr5_2:3749620-3749639 None:intergenic 30.0%
CTTAGAACAGAGATCTAAAA+TGG + chr5_2:3749630-3749649 None:intergenic 30.0%
! GTTTGAGATTTAGATTCGTT+AGG + chr5_2:3749709-3749728 None:intergenic 30.0%
AAAAAAGAAGATGAAATGGG+AGG - chr5_2:3749867-3749886 Msa0750350:intron 30.0%
ATTGAATATTTACTTCGTCG+TGG - chr5_2:3750062-3750081 Msa0750350:intron 30.0%
! TCCCGATCAACTAATTTTCA+GGG - chr5_2:3749523-3749542 Msa0750350:CDS 35.0%
ACCCCTGAAAATTAGTTGAT+CGG + chr5_2:3749528-3749547 None:intergenic 35.0%
! AAAATGGGCTTATTGGGTTT+GGG + chr5_2:3749614-3749633 None:intergenic 35.0%
! TAAAATGGGCTTATTGGGTT+TGG + chr5_2:3749615-3749634 None:intergenic 35.0%
! GAGATCTAAAATGGGCTTAT+TGG + chr5_2:3749621-3749640 None:intergenic 35.0%
TTTAGATTCGTTAGGTGCTT+TGG + chr5_2:3749701-3749720 None:intergenic 35.0%
! TCCCGATCAACTAATTTTCA+GGG - chr5_2:3749523-3749542 Msa0750350:CDS 35.0%
ACCCCTGAAAATTAGTTGAT+CGG + chr5_2:3749528-3749547 None:intergenic 35.0%
! AAAATGGGCTTATTGGGTTT+GGG + chr5_2:3749614-3749633 None:intergenic 35.0%
! TAAAATGGGCTTATTGGGTT+TGG + chr5_2:3749615-3749634 None:intergenic 35.0%
! GAGATCTAAAATGGGCTTAT+TGG + chr5_2:3749621-3749640 None:intergenic 35.0%
TTTAGATTCGTTAGGTGCTT+TGG + chr5_2:3749701-3749720 None:intergenic 35.0%
!! AGGGGATTTTGAGAAACTTG+AGG + chr5_2:3749421-3749440 None:intergenic 40.0%
! CTCCCGATCAACTAATTTTC+AGG - chr5_2:3749522-3749541 Msa0750350:CDS 40.0%
CCCCTGAAAATTAGTTGATC+GGG + chr5_2:3749527-3749546 None:intergenic 40.0%
! CCCGATCAACTAATTTTCAG+GGG - chr5_2:3749524-3749543 Msa0750350:CDS 40.0%
!! ATTTTCAGGGGTTGAGCAAT+TGG - chr5_2:3749536-3749555 Msa0750350:CDS 40.0%
TTGGGTTTGGGCTCTTAAAT+AGG + chr5_2:3749602-3749621 None:intergenic 40.0%
TTGGAACACGAGTTTAGAAG+AGG + chr5_2:3749658-3749677 None:intergenic 40.0%
!!! TTTGTGGTGTTGTTGCGTTT+TGG + chr5_2:3749677-3749696 None:intergenic 40.0%
CAAGTGCTTAGCTTATACAG+AGG - chr5_2:3749924-3749943 Msa0750350:intron 40.0%
TTGCAATGAAGATAACCCAG+TGG + chr5_2:3750123-3750142 None:intergenic 40.0%
!! AGGGGATTTTGAGAAACTTG+AGG + chr5_2:3749421-3749440 None:intergenic 40.0%
! CTCCCGATCAACTAATTTTC+AGG - chr5_2:3749522-3749541 Msa0750350:CDS 40.0%
CCCCTGAAAATTAGTTGATC+GGG + chr5_2:3749527-3749546 None:intergenic 40.0%
! CCCGATCAACTAATTTTCAG+GGG - chr5_2:3749524-3749543 Msa0750350:CDS 40.0%
!! ATTTTCAGGGGTTGAGCAAT+TGG - chr5_2:3749536-3749555 Msa0750350:CDS 40.0%
TTGGGTTTGGGCTCTTAAAT+AGG + chr5_2:3749602-3749621 None:intergenic 40.0%
TTGGAACACGAGTTTAGAAG+AGG + chr5_2:3749658-3749677 None:intergenic 40.0%
!!! TTTGTGGTGTTGTTGCGTTT+TGG + chr5_2:3749677-3749696 None:intergenic 40.0%
CAAGTGCTTAGCTTATACAG+AGG - chr5_2:3749924-3749943 Msa0750350:intron 40.0%
TTGCAATGAAGATAACCCAG+TGG + chr5_2:3750123-3750142 None:intergenic 40.0%
!!! CGTTAGGTGCTTTGGTTTTG+TGG + chr5_2:3749693-3749712 None:intergenic 45.0%
! AATGAGAACGAGAAGAGCGT+AGG + chr5_2:3749738-3749757 None:intergenic 45.0%
TTCTCGTTCTCATTCCTCTC+AGG - chr5_2:3749744-3749763 Msa0750350:CDS 45.0%
CTTGATCAGCTTAACAACCC+TGG - chr5_2:3750095-3750114 Msa0750350:intron 45.0%
TTAACAACCCTGGTACCACT+GGG - chr5_2:3750105-3750124 Msa0750350:intron 45.0%
AAGATAACCCAGTGGTACCA+GGG + chr5_2:3750115-3750134 None:intergenic 45.0%
!!! CGTTAGGTGCTTTGGTTTTG+TGG + chr5_2:3749693-3749712 None:intergenic 45.0%
! AATGAGAACGAGAAGAGCGT+AGG + chr5_2:3749738-3749757 None:intergenic 45.0%
TTCTCGTTCTCATTCCTCTC+AGG - chr5_2:3749744-3749763 Msa0750350:CDS 45.0%
CTTGATCAGCTTAACAACCC+TGG - chr5_2:3750095-3750114 Msa0750350:intron 45.0%
TTAACAACCCTGGTACCACT+GGG - chr5_2:3750105-3750124 Msa0750350:intron 45.0%
AAGATAACCCAGTGGTACCA+GGG + chr5_2:3750115-3750134 None:intergenic 45.0%
TTCAGGGGTTGAGCAATTGG+AGG - chr5_2:3749539-3749558 Msa0750350:CDS 50.0%
TCAGGGGTTGAGCAATTGGA+GGG - chr5_2:3749540-3749559 Msa0750350:CDS 50.0%
ACCAGAGTGCAAAGGCTTTC+TGG - chr5_2:3749891-3749910 Msa0750350:intron 50.0%
TCCAGAAAGCCTTTGCACTC+TGG + chr5_2:3749895-3749914 None:intergenic 50.0%
CTTAACAACCCTGGTACCAC+TGG - chr5_2:3750104-3750123 Msa0750350:intron 50.0%
GAAGATAACCCAGTGGTACC+AGG + chr5_2:3750116-3750135 None:intergenic 50.0%
TTCAGGGGTTGAGCAATTGG+AGG - chr5_2:3749539-3749558 Msa0750350:CDS 50.0%
TCAGGGGTTGAGCAATTGGA+GGG - chr5_2:3749540-3749559 Msa0750350:CDS 50.0%
ACCAGAGTGCAAAGGCTTTC+TGG - chr5_2:3749891-3749910 Msa0750350:intron 50.0%
TCCAGAAAGCCTTTGCACTC+TGG + chr5_2:3749895-3749914 None:intergenic 50.0%
CTTAACAACCCTGGTACCAC+TGG - chr5_2:3750104-3750123 Msa0750350:intron 50.0%
GAAGATAACCCAGTGGTACC+AGG + chr5_2:3750116-3750135 None:intergenic 50.0%
CAGGGGTTGAGCAATTGGAG+GGG - chr5_2:3749541-3749560 Msa0750350:CDS 55.0%
TGGGAGGAACCAGAGTGCAA+AGG - chr5_2:3749883-3749902 Msa0750350:intron 55.0%
CAGGGGTTGAGCAATTGGAG+GGG - chr5_2:3749541-3749560 Msa0750350:CDS 55.0%
TGGGAGGAACCAGAGTGCAA+AGG - chr5_2:3749883-3749902 Msa0750350:intron 55.0%
GGGTTGAGCAATTGGAGGGG+CGG - chr5_2:3749544-3749563 Msa0750350:CDS 60.0%
GCGGCGTTCTTCGTCGTGTT+TGG + chr5_2:3749970-3749989 None:intergenic 60.0%
GGGTTGAGCAATTGGAGGGG+CGG - chr5_2:3749544-3749563 Msa0750350:CDS 60.0%
GCGGCGTTCTTCGTCGTGTT+TGG + chr5_2:3749970-3749989 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 3749302 3750357 3749302 ID=Msa0750350;Name=Msa0750350
chr5_2 mRNA 3749302 3750357 3749302 ID=Msa0750350-mRNA-1;Parent=Msa0750350;Name=Msa0750350-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0.5|0.5|1|1|2|194|104
chr5_2 exon 3749302 3749795 3749302 ID=Msa0750350-mRNA-1:exon:1401;Parent=Msa0750350-mRNA-1
chr5_2 exon 3750343 3750357 3750343 ID=Msa0750350-mRNA-1:exon:1400;Parent=Msa0750350-mRNA-1
chr5_2 CDS 3750343 3750357 3750343 ID=Msa0750350-mRNA-1:cds;Parent=Msa0750350-mRNA-1
chr5_2 CDS 3749496 3749795 3749496 ID=Msa0750350-mRNA-1:cds;Parent=Msa0750350-mRNA-1
chr5_2 three_prime_UTR 3749302 3749495 3749302 ID=Msa0750350-mRNA-1:three_prime_utr;Parent=Msa0750350-mRNA-1
Gene Sequence

>Msa0750350

ATGCATAGCATTACTAGAAAAAAAGAAGATGAAATGGGAGGAACCAGAGTGCAAAGGCTTTCTGGAATGCAAAAGCAAGTGCTTAGCTTATACAGAGGATTTCTACGCGCAGCACGTTCCAAACACGACGAAGAACGCCGCAACATAGAATCTTTAGTATCTCAAGAATTTCGCCGTAATTCAAAAGAAATTGATCGAAAGAATTTTCAATACATTGAATATTTACTTCGTCGTGGTCATAAACAGCTTGATCAGCTTAACAACCCTGGTACCACTGGGTTATCTTCATTGCAACTTCATTTATCTAAAGATTAA

Protein sequence

>Msa0750350

MHSITRKKEDEMGGTRVQRLSGMQKQVLSLYRGFLRAARSKHDEERRNIESLVSQEFRRNSKEIDRKNFQYIEYLLRRGHKQLDQLNNPGTTGLSSLQLHLSKD*