AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686230 MtrunA17_Chr4g0035051 97.647 85 2 0 1 85 1 85 3.19e-58 177
Msa0686230 MtrunA17_Chr4g0035031 86.047 86 12 0 1 86 1 86 6.49e-50 159
Msa0686230 MtrunA17_Chr4g0035001 80.469 128 19 1 4 131 2 123 1.28e-44 144
Msa0686230 MtrunA17_Chr2g0278661 55.056 89 39 1 1 89 1 88 1.02e-31 114
Msa0686230 MtrunA17_Chr4g0050781 52.874 87 40 1 1 87 1 86 6.74e-29 105
Msa0686230 MtrunA17_Chr4g0061451 48.276 87 44 1 1 87 36 121 1.93e-23 93.2
Msa0686230 MtrunA17_Chr7g0219021 46.154 91 45 1 1 91 37 123 2.80e-23 92.4
Msa0686230 MtrunA17_Chr3g0116631 48.148 81 42 0 8 88 206 286 3.81e-23 92.8
Msa0686230 MtrunA17_Chr3g0116631 39.506 81 49 0 9 89 110 190 3.69e-12 62.8
Msa0686230 MtrunA17_Chr1g0210241 50.617 81 40 0 7 87 6 86 8.52e-23 91.7
Msa0686230 MtrunA17_Chr3g0086861 44.706 85 47 0 3 87 39 123 1.69e-21 90.1
Msa0686230 MtrunA17_Chr4g0058151 46.914 81 43 0 8 88 216 296 2.58e-20 85.1
Msa0686230 MtrunA17_Chr4g0058151 39.286 84 51 0 7 90 121 204 1.26e-14 69.7
Msa0686230 MtrunA17_Chr1g0181111 48.750 80 41 0 9 88 197 276 2.15e-19 82.4
Msa0686230 MtrunA17_Chr3g0121631 48.544 103 52 1 9 111 37 138 2.29e-18 77.0
Msa0686230 MtrunA17_Chr7g0266751 37.500 96 57 1 6 98 30 125 9.41e-18 75.1
Msa0686230 MtrunA17_Chr3g0119311 39.024 82 50 0 9 90 37 118 1.78e-17 74.3
Msa0686230 MtrunA17_Chr3g0127751 41.379 87 50 1 9 95 27 112 3.60e-17 75.9
Msa0686230 MtrunA17_Chr4g0017401 40.594 101 48 4 5 95 12 110 3.76e-17 76.3
Msa0686230 MtrunA17_Chr1g0168601 39.024 82 50 0 4 85 149 230 5.88e-17 75.1
Msa0686230 MtrunA17_Chr3g0144311 41.111 90 49 2 9 95 30 118 1.23e-16 75.1
Msa0686230 MtrunA17_Chr5g0426311 46.053 76 40 1 9 84 17 91 1.29e-16 75.1
Msa0686230 MtrunA17_Chr3g0101061 37.374 99 60 1 9 107 41 137 1.79e-16 75.9
Msa0686230 MtrunA17_Chr7g0276531 52.542 59 28 0 9 67 19 77 4.87e-16 73.2
Msa0686230 MtrunA17_Chr8g0346011 33.582 134 74 4 9 129 38 169 9.20e-16 72.8
Msa0686230 MtrunA17_Chr6g0481071 36.782 87 55 0 9 95 96 182 1.40e-15 70.5
Msa0686230 MtrunA17_Chr3g0088511 43.836 73 41 0 9 81 33 105 1.84e-15 68.9
Msa0686230 MtrunA17_Chr2g0290191 37.975 79 47 1 9 87 5 81 5.46e-15 71.6
Msa0686230 MtrunA17_Chr4g0018581 34.351 131 79 3 10 138 453 578 6.23e-15 71.6
Msa0686230 MtrunA17_Chr5g0447761 35.354 99 62 1 9 107 7 103 8.17e-15 70.9
Msa0686230 MtrunA17_Chr5g0447761 32.184 87 58 1 9 95 107 192 4.27e-11 60.1
Msa0686230 MtrunA17_Chr7g0263001 39.241 79 48 0 9 87 36 114 1.67e-14 66.6
Msa0686230 MtrunA17_Chr1g0186351 34.177 79 50 1 9 87 7 83 1.77e-14 70.1
Msa0686230 MtrunA17_Chr1g0186351 28.387 155 97 2 9 150 113 266 3.73e-12 63.2
Msa0686230 MtrunA17_Chr7g0219031 40.909 66 39 0 9 74 41 106 1.08e-13 63.9
Msa0686230 MtrunA17_Chr3g0120721 33.071 127 67 2 9 117 107 233 1.57e-13 67.0
Msa0686230 MtrunA17_Chr3g0120721 41.558 77 36 1 9 85 19 86 1.36e-12 64.3
Msa0686230 MtrunA17_Chr6g0449481 38.554 83 49 1 9 91 46 126 3.88e-13 65.9
Msa0686230 MtrunA17_Chr6g0449481 32.692 104 67 1 9 109 134 237 1.23e-12 64.7
Msa0686230 MtrunA17_Chr2g0327921 35.366 82 53 0 9 90 8 89 4.92e-13 61.6
Msa0686230 MtrunA17_Chr4g0062931 34.568 162 91 6 10 165 9 161 7.48e-13 65.5
Msa0686230 MtrunA17_Chr3g0135111 36.735 98 56 2 9 102 11 106 8.94e-13 65.1
Msa0686230 MtrunA17_Chr4g0042161 38.095 84 46 2 11 91 54 134 1.11e-12 62.0
Msa0686230 MtrunA17_Chr4g0065741 34.783 92 59 1 9 100 72 162 1.67e-12 62.0
Msa0686230 MtrunA17_Chr7g0267571 36.667 90 57 0 4 93 208 297 2.22e-12 63.5
Msa0686230 MtrunA17_Chr4g0031091 38.356 73 45 0 9 81 34 106 3.05e-12 60.1
Msa0686230 MtrunA17_Chr5g0402191 40.000 80 44 2 9 87 5 81 3.03e-11 60.5
Msa0686230 MtrunA17_Chr5g0419351 38.554 83 51 0 10 92 12 94 4.07e-11 58.5
Msa0686230 MtrunA17_Chr3g0110211 38.889 90 54 1 3 91 107 196 6.84e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686230 AT2G21660.2 84.706 85 13 0 1 85 1 85 1.99e-48 154
Msa0686230 AT2G21660.1 84.706 85 13 0 1 85 1 85 3.34e-48 154
Msa0686230 AT4G39260.3 77.778 90 20 0 4 93 2 91 6.31e-48 150
Msa0686230 AT4G39260.2 75.610 82 20 0 4 85 2 83 7.14e-43 139
Msa0686230 AT4G39260.1 75.610 82 20 0 4 85 2 83 2.61e-42 139
Msa0686230 AT4G39260.4 76.119 67 16 0 4 70 2 68 3.31e-34 116
Msa0686230 AT3G26420.1 52.577 97 44 1 3 99 2 96 1.46e-30 111
Msa0686230 AT3G23830.2 50.602 83 41 0 9 91 36 118 1.64e-24 92.8
Msa0686230 AT3G23830.1 50.602 83 41 0 9 91 36 118 1.64e-24 92.8
Msa0686230 AT4G13850.2 50.602 83 41 0 9 91 36 118 5.28e-24 92.0
Msa0686230 AT5G61030.2 49.367 79 40 0 9 87 41 119 5.38e-24 95.5
Msa0686230 AT5G61030.1 49.367 79 40 0 9 87 41 119 5.38e-24 95.5
Msa0686230 AT4G13850.3 50.538 93 46 0 9 101 36 128 6.10e-23 89.0
Msa0686230 AT4G13850.1 50.538 93 46 0 9 101 36 128 8.70e-23 89.0
Msa0686230 AT5G50250.1 45.783 83 45 0 5 87 204 286 1.44e-21 89.0
Msa0686230 AT5G50250.1 43.373 83 47 0 7 89 112 194 4.60e-15 71.2
Msa0686230 AT4G13850.4 52.000 100 42 1 9 108 36 129 2.80e-21 84.3
Msa0686230 AT4G24770.1 43.902 82 46 0 8 89 244 325 2.87e-21 88.6
Msa0686230 AT4G24770.1 44.578 83 46 0 7 89 149 231 1.70e-15 72.8
Msa0686230 AT4G24770.2 43.902 82 46 0 8 89 244 325 2.87e-21 88.6
Msa0686230 AT4G24770.2 44.578 83 46 0 7 89 149 231 1.70e-15 72.8
Msa0686230 AT2G21690.1 51.087 92 39 2 1 87 1 91 4.54e-21 83.6
Msa0686230 AT3G53460.1 44.578 83 46 0 9 91 258 340 5.55e-21 88.2
Msa0686230 AT3G53460.3 44.578 83 46 0 9 91 258 340 5.55e-21 88.2
Msa0686230 AT3G53460.2 44.578 83 46 0 9 91 258 340 5.55e-21 88.2
Msa0686230 AT5G04280.1 49.367 79 40 0 9 87 8 86 5.59e-21 87.4
Msa0686230 AT3G53460.4 44.578 83 46 0 9 91 279 361 7.33e-21 87.8
Msa0686230 AT2G37220.1 44.444 81 45 0 9 89 205 285 7.47e-21 87.0
Msa0686230 AT2G37220.1 41.250 80 45 1 3 82 88 165 3.41e-11 60.5
Msa0686230 AT1G74230.1 52.632 76 35 1 9 84 35 109 4.16e-20 85.1
Msa0686230 AT1G60650.2 38.095 126 74 2 5 126 9 134 1.49e-19 83.6
Msa0686230 AT1G60650.1 38.095 126 74 2 5 126 9 134 1.49e-19 83.6
Msa0686230 AT5G06210.1 41.573 89 52 0 6 94 32 120 7.15e-19 78.6
Msa0686230 AT1G22330.1 44.037 109 52 4 9 110 18 124 3.62e-18 79.7
Msa0686230 AT1G76460.1 47.368 76 39 1 9 84 25 99 1.25e-17 78.2
Msa0686230 AT1G20880.2 46.053 76 40 1 9 84 25 99 2.07e-17 77.4
Msa0686230 AT1G20880.3 46.053 76 40 1 9 84 25 99 2.07e-17 77.4
Msa0686230 AT1G20880.1 46.053 76 40 1 9 84 25 99 2.07e-17 77.4
Msa0686230 AT2G46780.1 44.737 76 41 1 9 84 23 97 2.19e-17 78.2
Msa0686230 AT2G46780.2 44.737 76 41 1 9 84 23 97 2.19e-17 78.2
Msa0686230 AT1G22910.1 39.394 99 55 3 5 99 9 106 2.78e-16 73.9
Msa0686230 AT4G14300.1 37.778 90 54 1 9 98 7 94 3.00e-16 75.5
Msa0686230 AT4G14300.2 37.778 90 54 1 9 98 7 94 3.00e-16 75.5
Msa0686230 AT1G78260.1 44.444 81 43 2 5 84 13 92 3.34e-16 74.3
Msa0686230 AT1G22910.2 39.394 99 55 3 5 99 9 106 3.58e-16 73.6
Msa0686230 AT3G07810.2 40.506 79 45 1 9 87 7 83 4.52e-16 75.1
Msa0686230 AT3G07810.1 40.506 79 45 1 9 87 7 83 4.98e-16 75.1
Msa0686230 AT4G13860.1 43.243 74 42 0 9 82 4 77 5.43e-16 69.3
Msa0686230 AT1G78260.2 44.444 81 43 2 5 84 13 92 6.89e-16 73.2
Msa0686230 AT2G33410.1 34.831 89 56 1 9 97 7 93 9.21e-16 73.9
Msa0686230 AT2G33410.1 37.975 79 48 1 9 87 111 188 8.01e-12 62.8
Msa0686230 AT4G26650.2 39.241 79 46 1 9 87 13 89 1.82e-15 73.2
Msa0686230 AT4G26650.1 39.241 79 46 1 9 87 16 92 1.89e-15 73.2
Msa0686230 AT1G22910.3 39.394 99 55 3 5 99 9 106 1.95e-15 72.8
Msa0686230 AT3G13224.3 37.234 94 53 2 6 95 4 95 4.70e-15 71.6
Msa0686230 AT3G13224.3 32.653 98 63 1 9 106 101 195 7.53e-14 68.2
Msa0686230 AT1G33470.2 37.895 95 53 2 9 98 8 101 5.07e-15 70.5
Msa0686230 AT5G55550.5 34.545 110 69 2 9 118 7 113 5.10e-15 72.0
Msa0686230 AT5G55550.4 34.545 110 69 2 9 118 7 113 5.10e-15 72.0
Msa0686230 AT5G55550.9 34.545 110 69 2 9 118 7 113 5.10e-15 72.0
Msa0686230 AT5G55550.7 34.545 110 69 2 9 118 7 113 5.10e-15 72.0
Msa0686230 AT5G55550.1 34.545 110 69 2 9 118 7 113 5.10e-15 72.0
Msa0686230 AT1G33470.3 37.895 95 53 2 9 98 8 101 5.13e-15 70.5
Msa0686230 AT5G55550.2 34.545 110 69 2 9 118 7 113 5.43e-15 72.0
Msa0686230 AT5G55550.3 34.545 110 69 2 9 118 7 113 5.43e-15 72.0
Msa0686230 AT5G55550.6 34.545 110 69 2 9 118 7 113 5.43e-15 72.0
Msa0686230 AT5G55550.11 34.545 110 69 2 9 118 7 113 5.43e-15 72.0
Msa0686230 AT1G33470.1 37.895 95 53 2 9 98 8 101 5.52e-15 70.5
Msa0686230 AT3G13224.2 37.931 87 52 1 9 95 20 104 6.45e-15 71.2
Msa0686230 AT3G13224.2 34.091 88 57 1 3 89 103 190 3.81e-14 69.3
Msa0686230 AT5G59865.1 43.038 79 45 0 11 89 29 107 8.58e-15 67.0
Msa0686230 AT1G60000.1 39.024 82 50 0 4 85 173 254 8.60e-15 70.1
Msa0686230 AT5G19960.1 37.838 111 66 2 10 120 9 116 8.83e-15 70.9
Msa0686230 AT5G47320.1 41.558 77 45 0 9 85 32 108 1.24e-14 68.9
Msa0686230 AT2G14160.1 40.698 86 32 2 1 68 1 85 1.60e-14 65.5
Msa0686230 AT3G08000.1 41.667 84 49 0 9 92 42 125 3.24e-14 66.2
Msa0686230 AT1G01080.2 39.241 79 43 2 7 83 212 287 4.60e-14 68.6
Msa0686230 AT1G01080.1 39.241 79 43 2 7 83 211 286 4.61e-14 68.6
Msa0686230 AT3G54770.3 43.421 76 42 1 9 84 18 92 5.76e-14 67.8
Msa0686230 AT3G54770.1 43.421 76 42 1 9 84 18 92 6.25e-14 67.8
Msa0686230 AT5G55550.8 42.254 71 39 1 9 79 7 75 1.15e-13 68.2
Msa0686230 AT1G73530.1 36.250 80 51 0 9 88 78 157 1.34e-13 65.5
Msa0686230 AT2G37510.1 41.892 74 43 0 9 82 35 108 1.35e-13 64.7
Msa0686230 AT5G55550.10 42.254 71 39 1 9 79 7 75 1.38e-13 67.8
Msa0686230 AT5G51300.1 31.746 126 84 1 7 132 479 602 1.49e-13 67.8
Msa0686230 AT5G51300.2 31.746 126 84 1 7 132 479 602 1.49e-13 67.8
Msa0686230 AT5G51300.3 31.746 126 84 1 7 132 479 602 1.49e-13 67.8
Msa0686230 AT5G47620.1 36.709 79 48 1 9 87 7 83 1.78e-13 67.4
Msa0686230 AT5G47620.2 36.709 79 48 1 9 87 7 83 1.78e-13 67.4
Msa0686230 AT5G47620.5 36.709 79 48 1 9 87 7 83 1.78e-13 67.4
Msa0686230 AT3G13224.1 37.931 87 52 1 9 95 20 104 2.41e-13 65.9
Msa0686230 AT3G46020.1 38.462 78 48 0 9 86 8 85 3.40e-13 62.8
Msa0686230 AT1G17640.2 38.750 80 47 1 9 88 67 144 5.18e-13 65.9
Msa0686230 AT1G17640.2 36.585 82 52 0 9 90 158 239 6.56e-12 62.8
Msa0686230 AT1G17640.1 38.750 80 47 1 9 88 67 144 5.18e-13 65.9
Msa0686230 AT1G17640.1 36.585 82 52 0 9 90 158 239 6.56e-12 62.8
Msa0686230 AT1G17640.3 38.750 80 47 1 9 88 67 144 5.18e-13 65.9
Msa0686230 AT1G17640.3 36.585 82 52 0 9 90 158 239 6.56e-12 62.8
Msa0686230 AT5G53720.1 40.000 80 47 1 5 84 6 84 5.45e-13 62.0
Msa0686230 AT3G52150.2 38.554 83 51 0 2 84 171 253 7.76e-13 64.7
Msa0686230 AT3G52150.1 38.554 83 51 0 2 84 171 253 7.76e-13 64.7
Msa0686230 AT5G40490.1 43.210 81 44 1 9 89 43 121 1.52e-12 64.7
Msa0686230 AT5G40490.1 32.609 92 61 1 1 91 122 213 3.62e-11 60.8
Msa0686230 AT5G47620.4 49.020 51 26 0 9 59 7 57 2.41e-12 64.3
Msa0686230 AT5G53680.1 43.478 69 38 1 9 77 14 81 4.50e-12 61.2
Msa0686230 AT5G64200.2 38.462 78 48 0 8 85 16 93 7.76e-12 62.4
Msa0686230 AT5G64200.1 38.462 78 48 0 8 85 16 93 7.76e-12 62.4
Msa0686230 AT3G06970.1 25.758 132 89 3 9 140 13 135 9.77e-12 62.0
Msa0686230 AT5G54580.1 42.308 78 45 0 10 87 58 135 1.06e-11 60.1
Msa0686230 AT4G13860.2 44.068 59 33 0 24 82 2 60 1.61e-11 57.4
Msa0686230 AT3G47120.2 38.667 75 46 0 10 84 38 112 1.78e-11 61.2
Msa0686230 AT3G47120.1 39.189 74 45 0 11 84 39 112 1.90e-11 61.6
Msa0686230 AT5G11412.1 42.169 83 46 2 3 84 46 127 2.10e-11 59.7
Msa0686230 AT2G43370.1 41.096 73 43 0 10 82 66 138 2.61e-11 61.2
Msa0686230 AT2G41060.1 42.647 68 38 1 1 67 120 187 2.67e-11 61.2
Msa0686230 AT2G41060.3 42.647 68 38 1 1 67 120 187 2.67e-11 61.2
Msa0686230 AT2G41060.2 42.647 68 38 1 1 67 120 187 2.67e-11 61.2
Msa0686230 AT2G43370.2 41.667 72 42 0 11 82 67 138 3.97e-11 59.3
Msa0686230 AT3G52380.1 37.838 74 46 0 9 82 117 190 4.07e-11 60.5
Msa0686230 AT3G52380.1 35.714 84 54 0 4 87 215 298 9.03e-11 59.3
Msa0686230 AT1G01080.3 38.235 68 37 2 7 72 211 275 5.38e-11 60.1
Msa0686230 AT4G36960.1 42.857 63 36 0 6 68 1 63 6.01e-11 60.1
Msa0686230 AT4G36960.2 42.857 63 36 0 6 68 1 63 6.01e-11 60.1
Msa0686230 AT1G58470.2 39.189 74 40 2 8 77 6 78 9.98e-11 59.3
Msa0686230 AT1G58470.1 39.189 74 40 2 8 77 6 78 9.98e-11 59.3

Find 79 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAGGTGGTGGTGGATA+TGG 0.258697 4_4:+50556161 Msa0686230:CDS
GGAGGAGGTGGTGGTGGTTA+TGG 0.262639 4_4:+50556245 Msa0686230:CDS
ACTGGACGATCAAGAGGTTT+TGG 0.283486 4_4:+50555990 Msa0686230:CDS
GGCCGTGGTGGCGGTGGATA+TGG 0.293898 4_4:+50556137 Msa0686230:CDS
ATTATCAACGATCGTGAAAC+TGG 0.322450 4_4:+50555972 Msa0686230:CDS
GGTGGTTATGGAGGAGGAGG+TGG 0.328302 4_4:+50556233 Msa0686230:CDS
AGTGGTGGTGGAGGTGGTTA+TGG 0.334178 4_4:+50556221 Msa0686230:CDS
GGTTATGGAGGAGGAGGTGG+TGG 0.336090 4_4:+50556236 Msa0686230:CDS
GGAGGTGGTGGTGGTTATGG+TGG 0.347344 4_4:+50556248 Msa0686230:CDS
GGTGGATATGGTGGAGGAGG+TGG 0.349052 4_4:+50556149 Msa0686230:CDS
GGTGGTAGGGACCGTGGTTA+TGG 0.354584 4_4:+50556269 Msa0686230:CDS
GTGGTGGTTATGGTGGTGGT+AGG 0.359680 4_4:+50556255 Msa0686230:CDS
GGATATGGTGGAGGAGGTGG+TGG 0.365647 4_4:+50556152 Msa0686230:CDS
GGAGGTGGTGGTGGATATGG+CGG 0.376984 4_4:+50556164 Msa0686230:CDS
GGTGGAGGAGGCCGTGGTGG+CGG 0.386911 4_4:+50556128 Msa0686230:CDS
GGAGGAGGCCGTGGTGGCGG+TGG 0.397234 4_4:+50556131 Msa0686230:CDS
TATGGAGGAGGAGGTGGTGG+TGG 0.400299 4_4:+50556239 Msa0686230:CDS
CAGAGCCGTGGTAGCGGTGG+TGG 0.401834 4_4:+50556110 Msa0686230:CDS
TATGGTGGAGGAGGTGGTGG+TGG 0.404828 4_4:+50556155 Msa0686230:CDS
GGTGGAGGTGGTTATGGAGG+AGG 0.415959 4_4:+50556227 Msa0686230:CDS
GGCGGTGGATATGGTGGAGG+AGG 0.419206 4_4:+50556146 Msa0686230:CDS
GTGGAGATGGTGGAGGAAGC+TGG 0.423465 4_4:+50556330 Msa0686230:CDS
AGCGGTGGTGGAGGAGGCCG+TGG 0.429688 4_4:+50556122 Msa0686230:CDS
GGAGGTGGTTATGGAGGAGG+AGG 0.430587 4_4:+50556230 Msa0686230:CDS
AGGGATGAACGGTTCAGATA+TGG 0.435664 4_4:+50556058 Msa0686230:CDS
GCTTCGTCGGAGGTCTTGCA+TGG 0.446995 4_4:+50555572 Msa0686230:CDS
GGTGGTGGTGGATATGGCGG+AGG 0.453193 4_4:+50556167 Msa0686230:CDS
GGTGGTGGATATGGCGGAGG+TGG 0.460996 4_4:+50556170 Msa0686230:CDS
GGTGGTGGAGGTGGTTATGG+AGG 0.464167 4_4:+50556224 Msa0686230:CDS
TATTCTTCACGCGGTGGAGA+TGG 0.477424 4_4:+50556317 Msa0686230:CDS
CGTGGTAGCGGTGGTGGAGG+AGG 0.477687 4_4:+50556116 Msa0686230:CDS
TGGTGGTTATGGTGGTGGTA+GGG 0.478980 4_4:+50556256 Msa0686230:CDS
GGTGGTGGTGGTTATGGTGG+TGG 0.479742 4_4:+50556251 Msa0686230:CDS
AGCCGTGGTAGCGGTGGTGG+AGG 0.481554 4_4:+50556113 Msa0686230:CDS
GGTTATGGTGGTGGAGATGA+TGG 0.485104 4_4:+50556284 Msa0686230:CDS
GTTGAGTACCGTTGCTTCGT+CGG 0.485717 4_4:+50555559 Msa0686230:CDS
AGAAGCAGTGGTGGTGGAGG+TGG 0.500860 4_4:+50556215 Msa0686230:CDS
TCGATGAGAGATGCGATTGA+AGG 0.506178 4_4:+50556038 Msa0686230:CDS
TACAGCAGAAGCAGTGGTGG+TGG 0.506540 4_4:+50556209 Msa0686230:CDS
GGCGGAGGTGGTCGCCGCGA+GGG 0.508855 4_4:+50556182 Msa0686230:CDS
AGCAGAAGCAGTGGTGGTGG+AGG 0.523650 4_4:+50556212 Msa0686230:CDS
AGGGACCGTGGTTATGGTGG+TGG 0.524349 4_4:+50556275 Msa0686230:CDS
GAGAAAGCCTTCTCTCAATA+CGG 0.531059 4_4:+50555616 Msa0686230:CDS
CGATGAGAGATGCGATTGAA+GGG 0.538221 4_4:+50556039 Msa0686230:CDS
TCTTCACGCGGTGGAGATGG+TGG 0.540242 4_4:+50556320 Msa0686230:CDS
CGTGAAACTGGACGATCAAG+AGG 0.551662 4_4:+50555984 Msa0686230:CDS
CTTCGTCGGAGGTCTTGCAT+GGG 0.553297 4_4:+50555573 Msa0686230:CDS
GAAGCTCAGAGCCGTGGTAG+CGG 0.559132 4_4:+50556104 Msa0686230:CDS
CGTGGTGGCGGTGGATATGG+TGG 0.562612 4_4:+50556140 Msa0686230:CDS
TCAACATCTGCAGAAGCCAT+TGG 0.563245 4_4:-50555541 None:intergenic
GGAGGTGGTCGCCGCGAGGG+TGG 0.563434 4_4:+50556185 Msa0686230:CDS
CGGAGAAATCATCGATTCGA+AGG 0.564623 4_4:+50555636 Msa0686230:CDS
GCTCAGAGCCGTGGTAGCGG+TGG 0.568719 4_4:+50556107 Msa0686230:CDS
GGTAGGGACCGTGGTTATGG+TGG 0.569427 4_4:+50556272 Msa0686230:CDS
GATTTCTCCGTATTGAGAGA+AGG 0.569467 4_4:-50555623 None:intergenic
GGTGGCGGTGGATATGGTGG+AGG 0.569854 4_4:+50556143 Msa0686230:CDS
CACCATATCCACCGCCACCA+CGG 0.571564 4_4:-50556139 None:intergenic
TCCACCGCGTGAAGAATAGC+GGG 0.572176 4_4:-50556312 None:intergenic
TATGGTGGTGGAGATGATGG+TGG 0.576911 4_4:+50556287 Msa0686230:CDS
CTCCTCCACCACCGCTACCA+CGG 0.595147 4_4:-50556115 None:intergenic
GGTGGTGGAGGAGGCCGTGG+TGG 0.601880 4_4:+50556125 Msa0686230:CDS
CAAGACCTCCGACGAAGCAA+CGG 0.604652 4_4:-50555567 None:intergenic
TCCCGCTATTCTTCACGCGG+TGG 0.608702 4_4:+50556311 Msa0686230:CDS
TCACGCGGTGGAGATGGTGG+AGG 0.610396 4_4:+50556323 Msa0686230:CDS
CTCCACCGCGTGAAGAATAG+CGG 0.613449 4_4:-50556313 None:intergenic
GAGTACCGTTGCTTCGTCGG+AGG 0.615538 4_4:+50555562 Msa0686230:CDS
GGTTACAGCAGAAGCAGTGG+TGG 0.617302 4_4:+50556206 Msa0686230:CDS
TGGCGGAGGTGGTCGCCGCG+AGG 0.618580 4_4:+50556181 Msa0686230:CDS
CTCGAGAGCATCGCTATCGG+TGG 0.619837 4_4:-50555596 None:intergenic
TTTCTCGAGAGCATCGCTAT+CGG 0.620818 4_4:-50555599 None:intergenic
GGTGGTTACAGCAGAAGCAG+TGG 0.635095 4_4:+50556203 Msa0686230:CDS
TATGGTGGTGGTAGGGACCG+TGG 0.636326 4_4:+50556263 Msa0686230:CDS
GATGCGATTGAAGGGATGAA+CGG 0.651599 4_4:+50556047 Msa0686230:CDS
ACGGTTCAGATATGGATGGA+AGG 0.652700 4_4:+50556066 Msa0686230:CDS
ATGAACGGTTCAGATATGGA+TGG 0.654412 4_4:+50556062 Msa0686230:CDS
GTGAACGAAGCTCAGAGCCG+TGG 0.656027 4_4:+50556098 Msa0686230:CDS
GGTTCCCGCTATTCTTCACG+CGG 0.677830 4_4:+50556308 Msa0686230:CDS
CATCTCCACCACCATAACCA+CGG 0.721401 4_4:-50556280 None:intergenic
TTCTGCTGTAACCACCCTCG+CGG 0.741441 4_4:-50556196 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTTTACTGTAAAATCTA+AGG + chr4_4:50555504-50555523 Msa0686230:five_prime_UTR 20.0%
!!! TCTTTTTACTGTAAAATCTA+AGG + chr4_4:50555504-50555523 Msa0686230:five_prime_UTR 20.0%
!!! GGTTTTAGTTTTTTCACCAA+TGG + chr4_4:50555525-50555544 Msa0686230:exon 30.0%
!!! GGTTTTAGTTTTTTCACCAA+TGG + chr4_4:50555525-50555544 Msa0686230:exon 30.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_4:50555690-50555709 Msa0686230:intron 35.0%
GGATTTCTCATGAAACCTTT+CGG + chr4_4:50555711-50555730 Msa0686230:intron 35.0%
! ATTTTGTTACTCACCCCAAT+AGG + chr4_4:50555829-50555848 Msa0686230:intron 35.0%
TGAAGATGAACGTACCTATT+GGG - chr4_4:50555846-50555865 None:intergenic 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_4:50555847-50555866 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_4:50555873-50555892 Msa0686230:intron 35.0%
AAGATCGATCGGTTACTATA+TGG + chr4_4:50555884-50555903 Msa0686230:intron 35.0%
ATTATCAACGATCGTGAAAC+TGG + chr4_4:50555972-50555991 Msa0686230:CDS 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_4:50555690-50555709 Msa0686230:intron 35.0%
GGATTTCTCATGAAACCTTT+CGG + chr4_4:50555711-50555730 Msa0686230:intron 35.0%
! ATTTTGTTACTCACCCCAAT+AGG + chr4_4:50555829-50555848 Msa0686230:intron 35.0%
TGAAGATGAACGTACCTATT+GGG - chr4_4:50555846-50555865 None:intergenic 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_4:50555847-50555866 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_4:50555873-50555892 Msa0686230:intron 35.0%
AAGATCGATCGGTTACTATA+TGG + chr4_4:50555884-50555903 Msa0686230:intron 35.0%
ATTATCAACGATCGTGAAAC+TGG + chr4_4:50555972-50555991 Msa0686230:CDS 35.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_4:50555616-50555635 Msa0686230:CDS 40.0%
GATTTCTCCGTATTGAGAGA+AGG - chr4_4:50555626-50555645 None:intergenic 40.0%
! CAGAGTATCACAGAACAGAT+CGG - chr4_4:50555748-50555767 None:intergenic 40.0%
GAAGATGAACGTACCTATTG+GGG - chr4_4:50555845-50555864 None:intergenic 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_4:50555877-50555896 None:intergenic 40.0%
ATGAACGGTTCAGATATGGA+TGG + chr4_4:50556062-50556081 Msa0686230:CDS 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_4:50555616-50555635 Msa0686230:CDS 40.0%
GATTTCTCCGTATTGAGAGA+AGG - chr4_4:50555626-50555645 None:intergenic 40.0%
! CAGAGTATCACAGAACAGAT+CGG - chr4_4:50555748-50555767 None:intergenic 40.0%
GAAGATGAACGTACCTATTG+GGG - chr4_4:50555845-50555864 None:intergenic 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_4:50555877-50555896 None:intergenic 40.0%
ATGAACGGTTCAGATATGGA+TGG + chr4_4:50556062-50556081 Msa0686230:CDS 40.0%
TCAACATCTGCAGAAGCCAT+TGG - chr4_4:50555544-50555563 None:intergenic 45.0%
!! TTTCTCGAGAGCATCGCTAT+CGG - chr4_4:50555602-50555621 None:intergenic 45.0%
!! CGGAGAAATCATCGATTCGA+AGG + chr4_4:50555636-50555655 Msa0686230:CDS 45.0%
!! TTTGTGTTGCTTGCAGGCTT+CGG + chr4_4:50555663-50555682 Msa0686230:intron 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_4:50555990-50556009 Msa0686230:CDS 45.0%
TCGATGAGAGATGCGATTGA+AGG + chr4_4:50556038-50556057 Msa0686230:CDS 45.0%
CGATGAGAGATGCGATTGAA+GGG + chr4_4:50556039-50556058 Msa0686230:CDS 45.0%
GATGCGATTGAAGGGATGAA+CGG + chr4_4:50556047-50556066 Msa0686230:CDS 45.0%
AGGGATGAACGGTTCAGATA+TGG + chr4_4:50556058-50556077 Msa0686230:CDS 45.0%
ACGGTTCAGATATGGATGGA+AGG + chr4_4:50556066-50556085 Msa0686230:CDS 45.0%
TCAACATCTGCAGAAGCCAT+TGG - chr4_4:50555544-50555563 None:intergenic 45.0%
!! TTTCTCGAGAGCATCGCTAT+CGG - chr4_4:50555602-50555621 None:intergenic 45.0%
!! CGGAGAAATCATCGATTCGA+AGG + chr4_4:50555636-50555655 Msa0686230:CDS 45.0%
!! TTTGTGTTGCTTGCAGGCTT+CGG + chr4_4:50555663-50555682 Msa0686230:intron 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_4:50555990-50556009 Msa0686230:CDS 45.0%
TCGATGAGAGATGCGATTGA+AGG + chr4_4:50556038-50556057 Msa0686230:CDS 45.0%
CGATGAGAGATGCGATTGAA+GGG + chr4_4:50556039-50556058 Msa0686230:CDS 45.0%
GATGCGATTGAAGGGATGAA+CGG + chr4_4:50556047-50556066 Msa0686230:CDS 45.0%
AGGGATGAACGGTTCAGATA+TGG + chr4_4:50556058-50556077 Msa0686230:CDS 45.0%
ACGGTTCAGATATGGATGGA+AGG + chr4_4:50556066-50556085 Msa0686230:CDS 45.0%
! GTTGAGTACCGTTGCTTCGT+CGG + chr4_4:50555559-50555578 Msa0686230:CDS 50.0%
!! GGTCTGTTTGTGTTGCTTGC+AGG + chr4_4:50555657-50555676 Msa0686230:intron 50.0%
TCGGAGATCACAGATCCGAA+AGG - chr4_4:50555729-50555748 None:intergenic 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_4:50555984-50556003 Msa0686230:CDS 50.0%
!! TGGTGGTTATGGTGGTGGTA+GGG + chr4_4:50556256-50556275 Msa0686230:CDS 50.0%
CATCTCCACCACCATAACCA+CGG - chr4_4:50556283-50556302 None:intergenic 50.0%
!! GGTTATGGTGGTGGAGATGA+TGG + chr4_4:50556284-50556303 Msa0686230:CDS 50.0%
!! TATGGTGGTGGAGATGATGG+TGG + chr4_4:50556287-50556306 Msa0686230:CDS 50.0%
TATTCTTCACGCGGTGGAGA+TGG + chr4_4:50556317-50556336 Msa0686230:CDS 50.0%
! GTTGAGTACCGTTGCTTCGT+CGG + chr4_4:50555559-50555578 Msa0686230:CDS 50.0%
!! GGTCTGTTTGTGTTGCTTGC+AGG + chr4_4:50555657-50555676 Msa0686230:intron 50.0%
TCGGAGATCACAGATCCGAA+AGG - chr4_4:50555729-50555748 None:intergenic 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_4:50555984-50556003 Msa0686230:CDS 50.0%
!! TGGTGGTTATGGTGGTGGTA+GGG + chr4_4:50556256-50556275 Msa0686230:CDS 50.0%
CATCTCCACCACCATAACCA+CGG - chr4_4:50556283-50556302 None:intergenic 50.0%
!! GGTTATGGTGGTGGAGATGA+TGG + chr4_4:50556284-50556303 Msa0686230:CDS 50.0%
!! TATGGTGGTGGAGATGATGG+TGG + chr4_4:50556287-50556306 Msa0686230:CDS 50.0%
TATTCTTCACGCGGTGGAGA+TGG + chr4_4:50556317-50556336 Msa0686230:CDS 50.0%
CAAGACCTCCGACGAAGCAA+CGG - chr4_4:50555570-50555589 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_4:50555573-50555592 Msa0686230:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_4:50555668-50555687 Msa0686230:intron 55.0%
TTCTGCTGTAACCACCCTCG+CGG - chr4_4:50556199-50556218 None:intergenic 55.0%
GGTGGTTACAGCAGAAGCAG+TGG + chr4_4:50556203-50556222 Msa0686230:CDS 55.0%
GGTTACAGCAGAAGCAGTGG+TGG + chr4_4:50556206-50556225 Msa0686230:CDS 55.0%
TACAGCAGAAGCAGTGGTGG+TGG + chr4_4:50556209-50556228 Msa0686230:CDS 55.0%
!! AGTGGTGGTGGAGGTGGTTA+TGG + chr4_4:50556221-50556240 Msa0686230:CDS 55.0%
!! GTGGTGGTTATGGTGGTGGT+AGG + chr4_4:50556255-50556274 Msa0686230:CDS 55.0%
GGTTCCCGCTATTCTTCACG+CGG + chr4_4:50556308-50556327 Msa0686230:CDS 55.0%
TCCACCGCGTGAAGAATAGC+GGG - chr4_4:50556315-50556334 None:intergenic 55.0%
CTCCACCGCGTGAAGAATAG+CGG - chr4_4:50556316-50556335 None:intergenic 55.0%
CAAGACCTCCGACGAAGCAA+CGG - chr4_4:50555570-50555589 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_4:50555573-50555592 Msa0686230:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_4:50555668-50555687 Msa0686230:intron 55.0%
TTCTGCTGTAACCACCCTCG+CGG - chr4_4:50556199-50556218 None:intergenic 55.0%
GGTGGTTACAGCAGAAGCAG+TGG + chr4_4:50556203-50556222 Msa0686230:CDS 55.0%
GGTTACAGCAGAAGCAGTGG+TGG + chr4_4:50556206-50556225 Msa0686230:CDS 55.0%
TACAGCAGAAGCAGTGGTGG+TGG + chr4_4:50556209-50556228 Msa0686230:CDS 55.0%
!! AGTGGTGGTGGAGGTGGTTA+TGG + chr4_4:50556221-50556240 Msa0686230:CDS 55.0%
!! GTGGTGGTTATGGTGGTGGT+AGG + chr4_4:50556255-50556274 Msa0686230:CDS 55.0%
GGTTCCCGCTATTCTTCACG+CGG + chr4_4:50556308-50556327 Msa0686230:CDS 55.0%
TCCACCGCGTGAAGAATAGC+GGG - chr4_4:50556315-50556334 None:intergenic 55.0%
CTCCACCGCGTGAAGAATAG+CGG - chr4_4:50556316-50556335 None:intergenic 55.0%
! GAGTACCGTTGCTTCGTCGG+AGG + chr4_4:50555562-50555581 Msa0686230:CDS 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_4:50555572-50555591 Msa0686230:CDS 60.0%
!! CTCGAGAGCATCGCTATCGG+TGG - chr4_4:50555599-50555618 None:intergenic 60.0%
GTGAACGAAGCTCAGAGCCG+TGG + chr4_4:50556098-50556117 Msa0686230:CDS 60.0%
GAAGCTCAGAGCCGTGGTAG+CGG + chr4_4:50556104-50556123 Msa0686230:CDS 60.0%
CACCATATCCACCGCCACCA+CGG - chr4_4:50556142-50556161 None:intergenic 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_4:50556149-50556168 Msa0686230:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_4:50556152-50556171 Msa0686230:CDS 60.0%
! TATGGTGGAGGAGGTGGTGG+TGG + chr4_4:50556155-50556174 Msa0686230:CDS 60.0%
!! GGAGGAGGTGGTGGTGGATA+TGG + chr4_4:50556161-50556180 Msa0686230:CDS 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG + chr4_4:50556164-50556183 Msa0686230:CDS 60.0%
!! AGCAGAAGCAGTGGTGGTGG+AGG + chr4_4:50556212-50556231 Msa0686230:CDS 60.0%
!! AGAAGCAGTGGTGGTGGAGG+TGG + chr4_4:50556215-50556234 Msa0686230:CDS 60.0%
!! GGTGGTGGAGGTGGTTATGG+AGG + chr4_4:50556224-50556243 Msa0686230:CDS 60.0%
GGTGGAGGTGGTTATGGAGG+AGG + chr4_4:50556227-50556246 Msa0686230:CDS 60.0%
GGAGGTGGTTATGGAGGAGG+AGG + chr4_4:50556230-50556249 Msa0686230:CDS 60.0%
GGTGGTTATGGAGGAGGAGG+TGG + chr4_4:50556233-50556252 Msa0686230:CDS 60.0%
GGTTATGGAGGAGGAGGTGG+TGG + chr4_4:50556236-50556255 Msa0686230:CDS 60.0%
! TATGGAGGAGGAGGTGGTGG+TGG + chr4_4:50556239-50556258 Msa0686230:CDS 60.0%
!! GGAGGAGGTGGTGGTGGTTA+TGG + chr4_4:50556245-50556264 Msa0686230:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_4:50556248-50556267 Msa0686230:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+TGG + chr4_4:50556251-50556270 Msa0686230:CDS 60.0%
!! TATGGTGGTGGTAGGGACCG+TGG + chr4_4:50556263-50556282 Msa0686230:CDS 60.0%
GGTGGTAGGGACCGTGGTTA+TGG + chr4_4:50556269-50556288 Msa0686230:CDS 60.0%
GGTAGGGACCGTGGTTATGG+TGG + chr4_4:50556272-50556291 Msa0686230:CDS 60.0%
AGGGACCGTGGTTATGGTGG+TGG + chr4_4:50556275-50556294 Msa0686230:CDS 60.0%
TCCCGCTATTCTTCACGCGG+TGG + chr4_4:50556311-50556330 Msa0686230:CDS 60.0%
TCTTCACGCGGTGGAGATGG+TGG + chr4_4:50556320-50556339 Msa0686230:CDS 60.0%
!! GTGGAGATGGTGGAGGAAGC+TGG + chr4_4:50556330-50556349 Msa0686230:CDS 60.0%
! GAGTACCGTTGCTTCGTCGG+AGG + chr4_4:50555562-50555581 Msa0686230:CDS 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_4:50555572-50555591 Msa0686230:CDS 60.0%
!! CTCGAGAGCATCGCTATCGG+TGG - chr4_4:50555599-50555618 None:intergenic 60.0%
GTGAACGAAGCTCAGAGCCG+TGG + chr4_4:50556098-50556117 Msa0686230:CDS 60.0%
GAAGCTCAGAGCCGTGGTAG+CGG + chr4_4:50556104-50556123 Msa0686230:CDS 60.0%
CACCATATCCACCGCCACCA+CGG - chr4_4:50556142-50556161 None:intergenic 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_4:50556149-50556168 Msa0686230:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_4:50556152-50556171 Msa0686230:CDS 60.0%
! TATGGTGGAGGAGGTGGTGG+TGG + chr4_4:50556155-50556174 Msa0686230:CDS 60.0%
!! GGAGGAGGTGGTGGTGGATA+TGG + chr4_4:50556161-50556180 Msa0686230:CDS 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG + chr4_4:50556164-50556183 Msa0686230:CDS 60.0%
!! AGCAGAAGCAGTGGTGGTGG+AGG + chr4_4:50556212-50556231 Msa0686230:CDS 60.0%
!! AGAAGCAGTGGTGGTGGAGG+TGG + chr4_4:50556215-50556234 Msa0686230:CDS 60.0%
!! GGTGGTGGAGGTGGTTATGG+AGG + chr4_4:50556224-50556243 Msa0686230:CDS 60.0%
GGTGGAGGTGGTTATGGAGG+AGG + chr4_4:50556227-50556246 Msa0686230:CDS 60.0%
GGAGGTGGTTATGGAGGAGG+AGG + chr4_4:50556230-50556249 Msa0686230:CDS 60.0%
GGTGGTTATGGAGGAGGAGG+TGG + chr4_4:50556233-50556252 Msa0686230:CDS 60.0%
GGTTATGGAGGAGGAGGTGG+TGG + chr4_4:50556236-50556255 Msa0686230:CDS 60.0%
! TATGGAGGAGGAGGTGGTGG+TGG + chr4_4:50556239-50556258 Msa0686230:CDS 60.0%
!! GGAGGAGGTGGTGGTGGTTA+TGG + chr4_4:50556245-50556264 Msa0686230:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_4:50556248-50556267 Msa0686230:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+TGG + chr4_4:50556251-50556270 Msa0686230:CDS 60.0%
!! TATGGTGGTGGTAGGGACCG+TGG + chr4_4:50556263-50556282 Msa0686230:CDS 60.0%
GGTGGTAGGGACCGTGGTTA+TGG + chr4_4:50556269-50556288 Msa0686230:CDS 60.0%
GGTAGGGACCGTGGTTATGG+TGG + chr4_4:50556272-50556291 Msa0686230:CDS 60.0%
AGGGACCGTGGTTATGGTGG+TGG + chr4_4:50556275-50556294 Msa0686230:CDS 60.0%
TCCCGCTATTCTTCACGCGG+TGG + chr4_4:50556311-50556330 Msa0686230:CDS 60.0%
TCTTCACGCGGTGGAGATGG+TGG + chr4_4:50556320-50556339 Msa0686230:CDS 60.0%
!! GTGGAGATGGTGGAGGAAGC+TGG + chr4_4:50556330-50556349 Msa0686230:CDS 60.0%
CTCCTCCACCACCGCTACCA+CGG - chr4_4:50556118-50556137 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+TGG + chr4_4:50556140-50556159 Msa0686230:CDS 65.0%
! GGTGGCGGTGGATATGGTGG+AGG + chr4_4:50556143-50556162 Msa0686230:CDS 65.0%
! GGCGGTGGATATGGTGGAGG+AGG + chr4_4:50556146-50556165 Msa0686230:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG + chr4_4:50556167-50556186 Msa0686230:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG + chr4_4:50556170-50556189 Msa0686230:CDS 65.0%
! TCACGCGGTGGAGATGGTGG+AGG + chr4_4:50556323-50556342 Msa0686230:CDS 65.0%
CTCCTCCACCACCGCTACCA+CGG - chr4_4:50556118-50556137 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+TGG + chr4_4:50556140-50556159 Msa0686230:CDS 65.0%
! GGTGGCGGTGGATATGGTGG+AGG + chr4_4:50556143-50556162 Msa0686230:CDS 65.0%
! GGCGGTGGATATGGTGGAGG+AGG + chr4_4:50556146-50556165 Msa0686230:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG + chr4_4:50556167-50556186 Msa0686230:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG + chr4_4:50556170-50556189 Msa0686230:CDS 65.0%
! TCACGCGGTGGAGATGGTGG+AGG + chr4_4:50556323-50556342 Msa0686230:CDS 65.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_4:50556107-50556126 Msa0686230:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_4:50556110-50556129 Msa0686230:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+AGG + chr4_4:50556113-50556132 Msa0686230:CDS 70.0%
!! CGTGGTAGCGGTGGTGGAGG+AGG + chr4_4:50556116-50556135 Msa0686230:CDS 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_4:50556137-50556156 Msa0686230:CDS 70.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_4:50556107-50556126 Msa0686230:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_4:50556110-50556129 Msa0686230:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+AGG + chr4_4:50556113-50556132 Msa0686230:CDS 70.0%
!! CGTGGTAGCGGTGGTGGAGG+AGG + chr4_4:50556116-50556135 Msa0686230:CDS 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_4:50556137-50556156 Msa0686230:CDS 70.0%
!! AGCGGTGGTGGAGGAGGCCG+TGG + chr4_4:50556122-50556141 Msa0686230:CDS 75.0%
!! GGTGGTGGAGGAGGCCGTGG+TGG + chr4_4:50556125-50556144 Msa0686230:CDS 75.0%
GGTGGAGGAGGCCGTGGTGG+CGG + chr4_4:50556128-50556147 Msa0686230:CDS 75.0%
!! AGCGGTGGTGGAGGAGGCCG+TGG + chr4_4:50556122-50556141 Msa0686230:CDS 75.0%
!! GGTGGTGGAGGAGGCCGTGG+TGG + chr4_4:50556125-50556144 Msa0686230:CDS 75.0%
GGTGGAGGAGGCCGTGGTGG+CGG + chr4_4:50556128-50556147 Msa0686230:CDS 75.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_4:50556131-50556150 Msa0686230:CDS 80.0%
! TGGCGGAGGTGGTCGCCGCG+AGG + chr4_4:50556181-50556200 Msa0686230:CDS 80.0%
! GGCGGAGGTGGTCGCCGCGA+GGG + chr4_4:50556182-50556201 Msa0686230:CDS 80.0%
! GGAGGTGGTCGCCGCGAGGG+TGG + chr4_4:50556185-50556204 Msa0686230:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_4:50556131-50556150 Msa0686230:CDS 80.0%
! TGGCGGAGGTGGTCGCCGCG+AGG + chr4_4:50556181-50556200 Msa0686230:CDS 80.0%
! GGCGGAGGTGGTCGCCGCGA+GGG + chr4_4:50556182-50556201 Msa0686230:CDS 80.0%
! GGAGGTGGTCGCCGCGAGGG+TGG + chr4_4:50556185-50556204 Msa0686230:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50555468 50556361 50555468 ID=Msa0686230;Name=Msa0686230
chr4_4 mRNA 50555468 50556361 50555468 ID=Msa0686230-mRNA-1;Parent=Msa0686230;Name=Msa0686230-mRNA-1;_AED=0.14;_eAED=0.14;_QI=76|1|0.5|1|1|1|2|0|167
chr4_4 exon 50555468 50555657 50555468 ID=Msa0686230-mRNA-1:exon:12948;Parent=Msa0686230-mRNA-1
chr4_4 exon 50555972 50556361 50555972 ID=Msa0686230-mRNA-1:exon:12949;Parent=Msa0686230-mRNA-1
chr4_4 five_prime_UTR 50555468 50555543 50555468 ID=Msa0686230-mRNA-1:five_prime_utr;Parent=Msa0686230-mRNA-1
chr4_4 CDS 50555544 50555657 50555544 ID=Msa0686230-mRNA-1:cds;Parent=Msa0686230-mRNA-1
chr4_4 CDS 50555972 50556361 50555972 ID=Msa0686230-mRNA-1:cds;Parent=Msa0686230-mRNA-1
Gene Sequence

>Msa0686230

ATGGCTTCTGCAGATGTTGAGTACCGTTGCTTCGTCGGAGGTCTTGCATGGGCCACCGATAGCGATGCTCTCGAGAAAGCCTTCTCTCAATACGGAGAAATCATCGATTCGAAGATTATCAACGATCGTGAAACTGGACGATCAAGAGGTTTTGGATTTGTGACATTTGCTGATGAGAAATCGATGAGAGATGCGATTGAAGGGATGAACGGTTCAGATATGGATGGAAGGAACATTACTGTGAACGAAGCTCAGAGCCGTGGTAGCGGTGGTGGAGGAGGCCGTGGTGGCGGTGGATATGGTGGAGGAGGTGGTGGTGGATATGGCGGAGGTGGTCGCCGCGAGGGTGGTTACAGCAGAAGCAGTGGTGGTGGAGGTGGTTATGGAGGAGGAGGTGGTGGTGGTTATGGTGGTGGTAGGGACCGTGGTTATGGTGGTGGAGATGATGGTGGTTCCCGCTATTCTTCACGCGGTGGAGATGGTGGAGGAAGCTGGAGAAGTTAG
Protein sequence

>Msa0686230

MASADVEYRCFVGGLAWATDSDALEKAFSQYGEIIDSKIINDRETGRSRGFGFVTFADEKSMRDAIEGMNGSDMDGRNITVNEAQSRGSGGGGGRGGGGYGGGGGGGYGGGGRREGGYSRSSGGGGGYGGGGGGGYGGGRDRGYGGGDDGGSRYSSRGGDGGGSWRS*