AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686220 MtrunA17_Chr4g0035031 97.895 190 3 1 1 189 1 190 8.52e-110 311
Msa0686220 MtrunA17_Chr4g0035051 88.235 85 10 0 1 85 1 85 1.31e-52 164
Msa0686220 MtrunA17_Chr4g0035001 83.495 103 17 0 4 106 2 104 2.11e-46 150
Msa0686220 MtrunA17_Chr2g0278661 56.180 89 38 1 1 89 1 88 1.37e-31 114
Msa0686220 MtrunA17_Chr4g0050781 44.516 155 81 2 3 157 2 151 4.11e-25 97.1
Msa0686220 MtrunA17_Chr3g0086861 47.059 85 45 0 3 87 39 123 6.51e-23 94.7
Msa0686220 MtrunA17_Chr3g0116631 51.852 81 39 0 8 88 206 286 7.18e-23 92.8
Msa0686220 MtrunA17_Chr3g0116631 27.815 151 99 1 9 159 110 250 6.32e-12 62.8
Msa0686220 MtrunA17_Chr4g0061451 47.500 80 42 0 9 88 43 122 2.31e-22 90.9
Msa0686220 MtrunA17_Chr7g0219021 44.578 83 46 0 9 91 41 123 6.65e-22 89.4
Msa0686220 MtrunA17_Chr3g0121631 50.633 79 38 1 9 87 37 114 1.22e-20 83.6
Msa0686220 MtrunA17_Chr4g0058151 50.617 81 40 0 8 88 216 296 1.64e-20 86.7
Msa0686220 MtrunA17_Chr4g0058151 40.964 83 49 0 8 90 122 204 3.81e-14 68.9
Msa0686220 MtrunA17_Chr1g0181111 50.575 87 43 0 2 88 190 276 2.46e-18 80.5
Msa0686220 MtrunA17_Chr1g0181111 36.283 113 71 1 9 120 85 197 2.20e-11 61.2
Msa0686220 MtrunA17_Chr3g0119311 40.244 82 49 0 9 90 37 118 2.54e-18 77.0
Msa0686220 MtrunA17_Chr7g0266751 40.741 81 48 0 6 86 30 110 3.90e-17 74.3
Msa0686220 MtrunA17_Chr3g0144311 36.842 152 79 5 9 144 30 180 1.91e-16 75.1
Msa0686220 MtrunA17_Chr4g0017401 41.000 100 52 4 5 99 12 109 2.90e-16 74.7
Msa0686220 MtrunA17_Chr1g0168601 41.463 82 48 0 4 85 149 230 1.96e-15 71.6
Msa0686220 MtrunA17_Chr5g0447761 37.975 79 47 1 9 87 7 83 1.97e-15 73.6
Msa0686220 MtrunA17_Chr5g0447761 32.258 93 62 1 9 101 107 198 3.47e-11 61.2
Msa0686220 MtrunA17_Chr3g0101061 37.975 79 47 1 9 87 41 117 2.98e-15 73.2
Msa0686220 MtrunA17_Chr3g0101061 32.000 150 88 4 9 145 141 289 2.83e-11 61.6
Msa0686220 MtrunA17_Chr7g0276531 46.053 76 40 1 9 84 19 93 5.04e-15 70.9
Msa0686220 MtrunA17_Chr2g0327921 39.024 82 50 0 9 90 8 89 8.52e-15 66.6
Msa0686220 MtrunA17_Chr2g0290191 39.241 79 46 1 9 87 5 81 9.15e-15 71.6
Msa0686220 MtrunA17_Chr2g0290191 33.835 133 78 3 9 131 107 239 1.39e-12 65.1
Msa0686220 MtrunA17_Chr5g0426311 44.737 76 41 1 9 84 17 91 9.57e-15 70.5
Msa0686220 MtrunA17_Chr3g0127751 45.000 80 43 1 9 88 27 105 9.83e-15 70.1
Msa0686220 MtrunA17_Chr1g0186351 35.443 79 49 1 9 87 7 83 1.69e-14 70.9
Msa0686220 MtrunA17_Chr1g0186351 30.097 206 113 5 9 184 113 317 5.44e-11 60.5
Msa0686220 MtrunA17_Chr8g0346011 32.639 144 86 5 9 145 38 177 2.37e-14 69.7
Msa0686220 MtrunA17_Chr4g0065741 37.500 96 57 1 1 96 67 159 4.32e-14 66.6
Msa0686220 MtrunA17_Chr6g0449481 38.095 84 50 1 9 92 46 127 9.08e-14 68.6
Msa0686220 MtrunA17_Chr6g0449481 33.858 127 80 1 9 131 134 260 1.99e-11 61.6
Msa0686220 MtrunA17_Chr5g0406171 32.386 176 99 5 10 171 133 302 9.98e-14 67.8
Msa0686220 MtrunA17_Chr3g0120721 32.374 139 86 1 9 147 107 237 1.31e-13 67.8
Msa0686220 MtrunA17_Chr3g0120721 36.585 82 50 1 9 90 19 98 1.84e-13 67.4
Msa0686220 MtrunA17_Chr7g0263001 37.931 87 54 0 1 87 28 114 3.16e-13 63.9
Msa0686220 MtrunA17_Chr7g0219031 42.424 66 38 0 9 74 41 106 4.02e-13 63.2
Msa0686220 MtrunA17_Chr6g0481071 38.750 80 49 0 9 88 96 175 1.07e-12 63.5
Msa0686220 MtrunA17_Chr4g0042161 38.298 94 52 2 11 101 54 144 1.78e-12 62.0
Msa0686220 MtrunA17_Chr5g0402191 41.250 80 43 2 9 87 5 81 2.43e-12 64.3
Msa0686220 MtrunA17_Chr4g0018581 44.444 72 40 0 10 81 453 524 8.20e-12 63.2
Msa0686220 MtrunA17_Chr4g0031091 39.726 73 44 0 9 81 34 106 9.51e-12 59.3
Msa0686220 MtrunA17_Chr1g0198431 35.870 92 56 1 9 97 193 284 1.04e-11 62.0
Msa0686220 MtrunA17_Chr3g0110211 39.175 97 58 1 3 98 107 203 1.78e-11 62.0
Msa0686220 MtrunA17_Chr3g0135111 31.532 111 75 1 9 119 118 227 1.96e-11 61.6
Msa0686220 MtrunA17_Chr4g0062931 34.503 171 91 7 10 179 9 159 2.21e-11 62.0
Msa0686220 MtrunA17_Chr5g0419351 36.957 92 58 0 10 101 12 103 3.22e-11 59.3
Msa0686220 MtrunA17_Chr3g0088511 38.356 73 45 0 9 81 33 105 6.70e-11 57.8
Msa0686220 MtrunA17_Chr8g0360551 35.955 89 55 2 9 97 74 160 7.24e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686220 AT4G39260.3 77.778 90 20 0 4 93 2 91 5.05e-48 152
Msa0686220 AT2G21660.2 78.824 85 18 0 1 85 1 85 3.86e-45 147
Msa0686220 AT2G21660.1 78.824 85 18 0 1 85 1 85 6.45e-45 147
Msa0686220 AT4G39260.1 75.610 82 20 0 4 85 2 83 2.57e-42 140
Msa0686220 AT4G39260.2 71.171 111 29 1 4 114 2 109 1.42e-38 129
Msa0686220 AT4G39260.4 76.119 67 16 0 4 70 2 68 3.83e-34 117
Msa0686220 AT3G26420.1 54.167 96 43 1 3 98 2 96 3.63e-31 114
Msa0686220 AT4G13850.2 54.217 83 38 0 9 91 36 118 3.84e-25 95.9
Msa0686220 AT3G23830.2 52.475 101 43 1 9 109 36 131 7.53e-24 92.0
Msa0686220 AT3G23830.1 52.475 101 43 1 9 109 36 131 7.53e-24 92.0
Msa0686220 AT5G04280.1 47.475 99 47 1 9 102 8 106 9.18e-24 95.9
Msa0686220 AT4G13850.3 56.566 99 43 0 9 107 36 134 1.46e-23 91.3
Msa0686220 AT4G13850.1 57.426 101 43 0 9 109 36 136 2.82e-23 90.9
Msa0686220 AT4G13850.4 56.383 94 40 1 9 101 36 129 3.96e-23 89.7
Msa0686220 AT5G61030.2 49.367 79 40 0 9 87 41 119 1.97e-22 92.4
Msa0686220 AT5G61030.1 49.367 79 40 0 9 87 41 119 1.97e-22 92.4
Msa0686220 AT2G21690.1 50.000 90 40 1 3 87 2 91 2.64e-21 84.7
Msa0686220 AT4G24770.1 47.561 82 43 0 8 89 244 325 1.59e-20 87.4
Msa0686220 AT4G24770.1 45.783 83 45 0 7 89 149 231 8.99e-16 74.3
Msa0686220 AT4G24770.2 47.561 82 43 0 8 89 244 325 1.59e-20 87.4
Msa0686220 AT4G24770.2 45.783 83 45 0 7 89 149 231 8.99e-16 74.3
Msa0686220 AT5G06210.1 44.318 88 49 0 6 93 32 119 1.85e-20 83.6
Msa0686220 AT5G50250.1 48.193 83 43 0 5 87 204 286 1.08e-19 84.7
Msa0686220 AT5G50250.1 32.680 153 90 2 7 159 112 251 5.72e-16 74.3
Msa0686220 AT3G53460.1 45.783 83 45 0 9 91 258 340 1.88e-19 84.7
Msa0686220 AT3G53460.3 45.783 83 45 0 9 91 258 340 1.88e-19 84.7
Msa0686220 AT3G53460.2 45.783 83 45 0 9 91 258 340 1.88e-19 84.7
Msa0686220 AT2G37220.1 45.679 81 44 0 9 89 205 285 2.61e-19 83.6
Msa0686220 AT3G53460.4 45.783 83 45 0 9 91 279 361 2.66e-19 84.3
Msa0686220 AT1G60650.2 42.169 83 48 0 5 87 9 91 3.84e-19 83.2
Msa0686220 AT1G60650.1 42.169 83 48 0 5 87 9 91 3.84e-19 83.2
Msa0686220 AT1G74230.1 52.688 93 39 2 1 93 31 118 5.13e-19 82.8
Msa0686220 AT3G07810.1 41.772 79 44 1 9 87 7 83 2.47e-17 79.3
Msa0686220 AT3G07810.1 33.577 137 73 4 9 131 109 241 2.92e-12 64.7
Msa0686220 AT3G07810.2 41.772 79 44 1 9 87 7 83 2.55e-17 79.3
Msa0686220 AT3G07810.2 33.577 137 73 4 9 131 109 241 2.87e-12 64.7
Msa0686220 AT1G76460.1 48.684 76 38 1 9 84 25 99 5.07e-17 77.4
Msa0686220 AT1G22330.1 44.118 102 51 3 9 105 18 118 9.28e-17 76.6
Msa0686220 AT1G18630.1 46.154 78 42 0 9 86 37 114 1.40e-16 73.6
Msa0686220 AT5G47320.1 46.753 77 41 0 9 85 32 108 2.04e-16 74.3
Msa0686220 AT4G26650.2 34.286 105 67 1 9 113 13 115 2.09e-16 76.6
Msa0686220 AT4G26650.1 34.286 105 67 1 9 113 16 118 2.14e-16 76.6
Msa0686220 AT4G14300.1 40.506 79 45 1 9 87 7 83 3.03e-16 76.3
Msa0686220 AT4G14300.2 40.506 79 45 1 9 87 7 83 3.03e-16 76.3
Msa0686220 AT1G20880.2 46.053 76 40 1 9 84 25 99 6.07e-16 74.3
Msa0686220 AT1G20880.3 46.053 76 40 1 9 84 25 99 6.07e-16 74.3
Msa0686220 AT1G20880.1 46.053 76 40 1 9 84 25 99 6.07e-16 74.3
Msa0686220 AT3G46020.1 42.857 77 44 0 9 85 8 84 1.74e-15 69.3
Msa0686220 AT1G22910.1 40.404 99 54 3 5 99 9 106 2.00e-15 72.4
Msa0686220 AT2G33410.1 39.241 79 46 1 9 87 7 83 2.44e-15 73.6
Msa0686220 AT2G46780.1 46.053 76 40 1 9 84 23 97 2.61e-15 73.2
Msa0686220 AT2G46780.2 46.053 76 40 1 9 84 23 97 2.61e-15 73.2
Msa0686220 AT1G22910.2 40.404 99 54 3 5 99 9 106 2.73e-15 72.0
Msa0686220 AT3G13224.3 38.462 91 50 2 6 92 4 92 3.93e-15 72.8
Msa0686220 AT3G13224.3 32.955 88 58 1 3 89 94 181 9.10e-12 63.2
Msa0686220 AT3G13224.2 39.286 84 49 1 9 92 20 101 4.54e-15 72.4
Msa0686220 AT3G13224.2 32.955 88 58 1 3 89 103 190 2.77e-12 64.7
Msa0686220 AT5G55550.5 33.981 103 66 1 9 111 7 107 5.60e-15 72.4
Msa0686220 AT5G55550.4 33.981 103 66 1 9 111 7 107 5.60e-15 72.4
Msa0686220 AT5G55550.9 33.981 103 66 1 9 111 7 107 5.60e-15 72.4
Msa0686220 AT5G55550.7 33.981 103 66 1 9 111 7 107 5.60e-15 72.4
Msa0686220 AT5G55550.1 33.981 103 66 1 9 111 7 107 5.60e-15 72.4
Msa0686220 AT1G33470.3 43.421 76 42 1 9 84 8 82 6.17e-15 70.9
Msa0686220 AT5G55550.2 33.981 103 66 1 9 111 7 107 6.96e-15 72.4
Msa0686220 AT5G55550.3 33.981 103 66 1 9 111 7 107 6.96e-15 72.4
Msa0686220 AT5G55550.6 33.981 103 66 1 9 111 7 107 6.96e-15 72.4
Msa0686220 AT5G55550.11 33.981 103 66 1 9 111 7 107 6.96e-15 72.4
Msa0686220 AT1G33470.2 43.421 76 42 1 9 84 8 82 7.04e-15 70.9
Msa0686220 AT1G33470.1 43.421 76 42 1 9 84 8 82 7.50e-15 70.9
Msa0686220 AT1G17640.2 42.500 80 44 1 9 88 67 144 1.21e-14 71.2
Msa0686220 AT1G17640.1 42.500 80 44 1 9 88 67 144 1.21e-14 71.2
Msa0686220 AT1G17640.3 42.500 80 44 1 9 88 67 144 1.21e-14 71.2
Msa0686220 AT3G08000.1 43.678 87 43 2 9 92 42 125 1.68e-14 67.8
Msa0686220 AT1G22910.3 40.404 99 54 3 5 99 9 106 1.93e-14 70.9
Msa0686220 AT2G37510.1 45.946 74 40 0 9 82 35 108 2.35e-14 67.4
Msa0686220 AT5G59865.1 44.304 79 44 0 11 89 29 107 4.03e-14 65.9
Msa0686220 AT1G78260.1 43.210 81 44 2 5 84 13 92 5.34e-14 68.9
Msa0686220 AT3G54770.3 43.421 76 42 1 9 84 18 92 6.40e-14 68.6
Msa0686220 AT5G19960.1 36.134 119 64 3 10 127 9 116 7.52e-14 68.9
Msa0686220 AT3G54770.1 43.421 76 42 1 9 84 18 92 8.07e-14 68.2
Msa0686220 AT1G78260.2 43.421 76 42 1 9 84 18 92 9.20e-14 68.2
Msa0686220 AT5G53720.1 42.683 82 46 1 3 84 4 84 2.69e-13 63.5
Msa0686220 AT5G40490.1 38.542 96 57 1 9 104 43 136 4.63e-13 67.0
Msa0686220 AT1G60000.1 40.244 82 49 0 4 85 173 254 4.82e-13 65.9
Msa0686220 AT5G55550.8 40.278 72 41 1 9 80 7 76 5.31e-13 67.0
Msa0686220 AT2G27330.1 29.670 91 63 1 11 100 24 114 6.14e-13 62.8
Msa0686220 AT3G13224.1 39.286 84 49 1 9 92 20 101 6.27e-13 65.5
Msa0686220 AT5G55550.10 40.278 72 41 1 9 80 7 76 6.79e-13 66.6
Msa0686220 AT3G14100.1 42.308 78 45 0 7 84 143 220 7.37e-13 66.2
Msa0686220 AT2G14160.1 39.286 84 32 2 1 66 1 83 8.40e-13 61.6
Msa0686220 AT5G47620.1 36.709 79 48 1 9 87 7 83 8.72e-13 66.2
Msa0686220 AT5G47620.2 36.709 79 48 1 9 87 7 83 8.72e-13 66.2
Msa0686220 AT5G47620.5 36.709 79 48 1 9 87 7 83 8.72e-13 66.2
Msa0686220 AT4G13860.1 40.541 74 44 0 9 82 4 77 1.54e-12 61.2
Msa0686220 AT1G01080.1 41.772 79 41 2 7 83 211 286 2.17e-12 64.7
Msa0686220 AT1G01080.2 41.772 79 41 2 7 83 212 287 2.18e-12 64.7
Msa0686220 AT1G54080.1 42.308 78 45 0 7 84 147 224 3.50e-12 64.3
Msa0686220 AT5G53680.1 46.377 69 36 1 9 77 14 81 6.32e-12 61.6
Msa0686220 AT5G04280.3 47.059 68 31 1 40 102 1 68 1.18e-11 62.4
Msa0686220 AT5G04280.4 47.059 68 31 1 40 102 1 68 1.18e-11 62.4
Msa0686220 AT5G04280.5 47.059 68 31 1 40 102 1 68 1.18e-11 62.4
Msa0686220 AT5G04280.2 47.059 68 31 1 40 102 1 68 1.18e-11 62.4
Msa0686220 AT5G47620.4 37.500 72 43 1 9 80 7 76 1.23e-11 62.8
Msa0686220 AT4G27000.1 43.243 74 41 1 7 79 172 245 2.18e-11 62.0
Msa0686220 AT5G51300.1 41.333 75 44 0 7 81 479 553 3.82e-11 61.6
Msa0686220 AT5G51300.2 41.333 75 44 0 7 81 479 553 3.82e-11 61.6
Msa0686220 AT5G51300.3 41.333 75 44 0 7 81 479 553 3.82e-11 61.6
Msa0686220 AT4G36960.1 36.782 87 53 1 6 90 1 87 4.27e-11 61.2
Msa0686220 AT4G36960.2 36.782 87 53 1 6 90 1 87 4.27e-11 61.2
Msa0686220 AT3G20930.1 35.000 80 52 0 9 88 283 362 5.32e-11 60.8
Msa0686220 AT2G22090.2 38.043 92 54 3 5 95 100 189 8.75e-11 60.1
Msa0686220 AT3G06970.1 30.973 113 71 3 9 120 13 119 9.21e-11 60.1
Msa0686220 AT2G22090.1 38.043 92 54 3 5 95 100 189 9.35e-11 60.1

Find 104 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGTGGAGGTGGTGGTTA+TGG 0.237735 4_4:+50543208 Msa0686220:CDS
ACTGGAAGATCAAGAGGTTT+TGG 0.244628 4_4:+50543040 Msa0686220:CDS
GGTGGAGGTGGTGGTGGTTA+TGG 0.247285 4_4:+50543334 Msa0686220:CDS
GGTAGAGGTGGCGGCGGCGG+TGG 0.247335 4_4:+50543184 Msa0686220:CDS
GGAGGTGGCGGTGGTGGATA+TGG 0.255891 4_4:+50543229 Msa0686220:CDS
TGTCTTTATTGTCCTGAATC+TGG 0.270319 4_4:+50543592 Msa0686220:three_prime_UTR
AGTGGTGGTGGTGGAGGATA+TGG 0.275624 4_4:+50543289 Msa0686220:CDS
ACTGGAGGGATTAGAAAATT+AGG 0.300294 4_4:+50543464 Msa0686220:exon
TTGGTTGAAGTTTAGTGATT+TGG 0.311002 4_4:+50543535 Msa0686220:three_prime_UTR
GGAGGTGGTGACCGTGGATA+TGG 0.314035 4_4:+50543313 Msa0686220:CDS
GGAGGTGGTGGTGGTTATGG+TGG 0.314652 4_4:+50543337 Msa0686220:CDS
GTTATCAATGATCGTGAAAC+TGG 0.330573 4_4:+50543022 Msa0686220:CDS
GGTGGCGGTGGTGGATATGG+TGG 0.346314 4_4:+50543232 Msa0686220:CDS
GGAGGTGGTGACCGTGGTTA+TGG 0.350450 4_4:+50543388 Msa0686220:CDS
GCTTCGTCGGTGGTCTTGCT+TGG 0.353153 4_4:+50542690 Msa0686220:CDS
GTGCTGATAGCGGTGGAAAC+TGG 0.354817 4_4:+50543446 Msa0686220:CDS
GGTGGTTATGGAGGTGGCGG+TGG 0.356362 4_4:+50543220 Msa0686220:CDS
GTTTATACAAAACCAGATTC+AGG 0.358098 4_4:-50543604 None:intergenic
GAGCAAGCCTTCTCTAAGTT+TGG 0.374142 4_4:+50542734 Msa0686220:CDS
GGTTATGGAGGTGGCGGTGG+TGG 0.376259 4_4:+50543223 Msa0686220:CDS
GTGGTTATGGTGGAGGTGGT+AGG 0.377057 4_4:+50543347 Msa0686220:CDS
GGAGGTAGAGGTGGCGGCGG+CGG 0.380285 4_4:+50543181 Msa0686220:CDS
CTTCGTCGGTGGTCTTGCTT+GGG 0.385202 4_4:+50542691 Msa0686220:CDS
GGTGGTAGGGACCGTGGATA+TGG 0.392248 4_4:+50543361 Msa0686220:CDS
GGTGGGTATGGTGGAGGTGG+TGG 0.400681 4_4:+50543202 Msa0686220:CDS
GGTGGCGGCGGCGGTGGGTA+TGG 0.403563 4_4:+50543190 Msa0686220:CDS
TGTTCTCATGTTGATTTAGT+TGG 0.409418 4_4:+50543516 Msa0686220:three_prime_UTR
CGTGGATATGGTGGTGGAGG+TGG 0.410093 4_4:+50543373 Msa0686220:CDS
GGTGGTGGTGGTTATGGTGG+AGG 0.410493 4_4:+50543340 Msa0686220:CDS
AGAAAATTAGGTCTTCAGTT+TGG 0.418164 4_4:+50543476 Msa0686220:exon
GGAGGTGGTGGTTATGGAGG+TGG 0.419282 4_4:+50543214 Msa0686220:CDS
GGCGGCGGCGGTGGGTATGG+TGG 0.419877 4_4:+50543193 Msa0686220:CDS
CGTGGATATGGTGGAGGTGG+TGG 0.422932 4_4:+50543325 Msa0686220:CDS
GGCGGTGGTGGATATGGTGG+AGG 0.424937 4_4:+50543235 Msa0686220:CDS
GGCCACCGATAGCCAAGCTC+TGG 0.439871 4_4:+50542712 Msa0686220:CDS
GGTGGAGGTGGTGGTTATGG+AGG 0.440305 4_4:+50543211 Msa0686220:CDS
CAGTCACGTGGCAGTGGTGG+AGG 0.442750 4_4:+50543160 Msa0686220:CDS
GGCAGTGGTGGAGGAGGTAG+AGG 0.451811 4_4:+50543169 Msa0686220:CDS
GGTGGTGGAGGATATGGTGG+AGG 0.455686 4_4:+50543295 Msa0686220:CDS
GGCGGTGGGTATGGTGGAGG+TGG 0.455761 4_4:+50543199 Msa0686220:CDS
GGTGGTGGATATGGTGGAGG+TGG 0.459945 4_4:+50543238 Msa0686220:CDS
GGTGGATATGGTGGAGGTGG+TGG 0.461684 4_4:+50543241 Msa0686220:CDS
GTATAAACGATTGATTGAAA+AGG 0.465420 4_4:+50543619 Msa0686220:three_prime_UTR
GGAGGTGGTGGTCGTCGTGA+TGG 0.466320 4_4:+50543253 Msa0686220:CDS
AGCAGAAGTGGTGGTGGTGG+AGG 0.470138 4_4:+50543283 Msa0686220:CDS
TGGTTATGGTGGAGGTGGTA+GGG 0.472480 4_4:+50543348 Msa0686220:CDS
GGCGGCGGTGGGTATGGTGG+AGG 0.475186 4_4:+50543196 Msa0686220:CDS
CGTGGTTATGGTGGTGGTGG+CGG 0.475722 4_4:+50543400 Msa0686220:CDS
GGATATGGTGGAGGTGGTGG+TGG 0.477834 4_4:+50543328 Msa0686220:CDS
AGAAGGCTTGCTCCAGAGCT+TGG 0.478074 4_4:-50542724 None:intergenic
GTAGAGGTGGCGGCGGCGGT+GGG 0.479681 4_4:+50543185 Msa0686220:CDS
GGTGGTGGTTATGGAGGTGG+CGG 0.480019 4_4:+50543217 Msa0686220:CDS
GGTGGAGGATATGGTGGAGG+TGG 0.483928 4_4:+50543298 Msa0686220:CDS
GGAGGAGGTAGAGGTGGCGG+CGG 0.497158 4_4:+50543178 Msa0686220:CDS
GGTGGTGGTGGAGGATATGG+TGG 0.497601 4_4:+50543292 Msa0686220:CDS
GGTGGTGGTTATGGTGGAGG+TGG 0.498780 4_4:+50543343 Msa0686220:CDS
TACAGCAGAAGTGGTGGTGG+TGG 0.500718 4_4:+50543280 Msa0686220:CDS
AAATTAGGTCTTCAGTTTGG+TGG 0.507215 4_4:+50543479 Msa0686220:three_prime_UTR
CAAGACCACCGACGAAGCAC+CGG 0.514431 4_4:-50542685 None:intergenic
GACCGTGGTTATGGTGGTGG+TGG 0.516242 4_4:+50543397 Msa0686220:CDS
GATCGCTACTCTCGCGGTGG+TGG 0.518871 4_4:+50543424 Msa0686220:CDS
GCTCAGTCACGTGGCAGTGG+TGG 0.524094 4_4:+50543157 Msa0686220:CDS
GGTGGAGGAGGTAGAGGTGG+CGG 0.531842 4_4:+50543175 Msa0686220:CDS
TCACGTGGCAGTGGTGGAGG+AGG 0.534421 4_4:+50543163 Msa0686220:CDS
GACCGTGGATATGGTGGAGG+TGG 0.539518 4_4:+50543322 Msa0686220:CDS
AATTGAAGAGATGAATGGTC+AGG 0.541125 4_4:+50543102 Msa0686220:CDS
GAAGCTCAGTCACGTGGCAG+TGG 0.541791 4_4:+50543154 Msa0686220:CDS
GACCGTGGATATGGTGGTGG+AGG 0.547656 4_4:+50543370 Msa0686220:CDS
AGGGACCGTGGATATGGTGG+TGG 0.548183 4_4:+50543367 Msa0686220:CDS
AGAACCCATCGAACTATTAG+CGG 0.549643 4_4:-50542648 None:intergenic
TGGTGAAATCACTGATTCCA+AGG 0.553599 4_4:+50542754 Msa0686220:CDS
TTGCTCCAGAGCTTGGCTAT+CGG 0.556107 4_4:-50542717 None:intergenic
GATGCAATTGAAGAGATGAA+TGG 0.556176 4_4:+50543097 Msa0686220:CDS
GGTGACCGTGGTTATGGTGG+TGG 0.556613 4_4:+50543394 Msa0686220:CDS
GGTGGTGACCGTGGATATGG+TGG 0.557481 4_4:+50543316 Msa0686220:CDS
GGTGGTGGTCGTCGTGATGG+TGG 0.559431 4_4:+50543256 Msa0686220:CDS
GGCGGTGATCGCTACTCTCG+CGG 0.560534 4_4:+50543418 Msa0686220:CDS
CGTGAAACTGGAAGATCAAG+AGG 0.562850 4_4:+50543034 Msa0686220:CDS
AGTGGTGGAGGAGGTAGAGG+TGG 0.566280 4_4:+50543172 Msa0686220:CDS
CGCGGTGGTGGTGCTGATAG+CGG 0.567365 4_4:+50543436 Msa0686220:CDS
CTCCAGAGCTTGGCTATCGG+TGG 0.571940 4_4:-50542714 None:intergenic
GGTGGTGACCGTGGTTATGG+TGG 0.575688 4_4:+50543391 Msa0686220:CDS
GATTTCACCAAACTTAGAGA+AGG 0.575852 4_4:-50542741 None:intergenic
GGTGGAGGTGGTAGGGACCG+TGG 0.578195 4_4:+50543355 Msa0686220:CDS
GGTGACCGTGGATATGGTGG+AGG 0.581643 4_4:+50543319 Msa0686220:CDS
GGATACAGCAGAAGTGGTGG+TGG 0.583391 4_4:+50543277 Msa0686220:CDS
GGTAGGGACCGTGGATATGG+TGG 0.583912 4_4:+50543364 Msa0686220:CDS
ATGAATGGTCAGGATATTGA+CGG 0.587239 4_4:+50543112 Msa0686220:CDS
GTTGAGTACCGGTGCTTCGT+CGG 0.588309 4_4:+50542677 Msa0686220:CDS
CTGATAGCGGTGGAAACTGG+AGG 0.605217 4_4:+50543449 Msa0686220:CDS
ATGGTCAGGATATTGACGGC+AGG 0.612223 4_4:+50543116 Msa0686220:CDS
GAGTACCGGTGCTTCGTCGG+TGG 0.614835 4_4:+50542680 Msa0686220:CDS
GTTCTTCTGATGTTGAGTAC+CGG 0.622558 4_4:+50542666 Msa0686220:CDS
GGTGGTGGAGGTGGTGACCG+TGG 0.625086 4_4:+50543382 Msa0686220:CDS
CACCACCTCCACCATATCCA+CGG 0.630646 4_4:-50543324 None:intergenic
CACCTCCACCACCATATCCA+CGG 0.630646 4_4:-50543372 None:intergenic
TATGGTGGAGGTGGTGACCG+TGG 0.638935 4_4:+50543307 Msa0686220:CDS
TGATAGCGGTGGAAACTGGA+GGG 0.650756 4_4:+50543450 Msa0686220:CDS
CGCCACCACCACCATAACCA+CGG 0.664909 4_4:-50543399 None:intergenic
GATGGTGGATACAGCAGAAG+TGG 0.665118 4_4:+50543271 Msa0686220:CDS
GTGAATGAAGCTCAGTCACG+TGG 0.667871 4_4:+50543148 Msa0686220:CDS
GGTGATCGCTACTCTCGCGG+TGG 0.687217 4_4:+50543421 Msa0686220:CDS
GGTGGATACAGCAGAAGTGG+TGG 0.687566 4_4:+50543274 Msa0686220:CDS
GGTGGTGGTGCTGATAGCGG+TGG 0.706620 4_4:+50543439 Msa0686220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAAGGATAATGAAAAAT+TGG + chr4_4:50543634-50543653 Msa0686220:three_prime_UTR 20.0%
!! TGAAAAGGATAATGAAAAAT+TGG + chr4_4:50543634-50543653 Msa0686220:three_prime_UTR 20.0%
! GTATAAACGATTGATTGAAA+AGG + chr4_4:50543619-50543638 Msa0686220:three_prime_UTR 25.0%
! GTATAAACGATTGATTGAAA+AGG + chr4_4:50543619-50543638 Msa0686220:three_prime_UTR 25.0%
TTAGCGGAAAAAAAAGATGA+AGG - chr4_4:50542635-50542654 None:intergenic 30.0%
! TGTGAATTTTCTTAGATCTG+AGG + chr4_4:50542985-50543004 Msa0686220:intron 30.0%
!!! ATCTGAGGTTATTTTTGTTC+AGG + chr4_4:50543000-50543019 Msa0686220:intron 30.0%
AGAAAATTAGGTCTTCAGTT+TGG + chr4_4:50543476-50543495 Msa0686220:exon 30.0%
!! TGTTCTCATGTTGATTTAGT+TGG + chr4_4:50543516-50543535 Msa0686220:three_prime_UTR 30.0%
TTGGTTGAAGTTTAGTGATT+TGG + chr4_4:50543535-50543554 Msa0686220:three_prime_UTR 30.0%
GTTTATACAAAACCAGATTC+AGG - chr4_4:50543607-50543626 None:intergenic 30.0%
TTAGCGGAAAAAAAAGATGA+AGG - chr4_4:50542635-50542654 None:intergenic 30.0%
! TGTGAATTTTCTTAGATCTG+AGG + chr4_4:50542985-50543004 Msa0686220:intron 30.0%
!!! ATCTGAGGTTATTTTTGTTC+AGG + chr4_4:50543000-50543019 Msa0686220:intron 30.0%
AGAAAATTAGGTCTTCAGTT+TGG + chr4_4:50543476-50543495 Msa0686220:exon 30.0%
!! TGTTCTCATGTTGATTTAGT+TGG + chr4_4:50543516-50543535 Msa0686220:three_prime_UTR 30.0%
TTGGTTGAAGTTTAGTGATT+TGG + chr4_4:50543535-50543554 Msa0686220:three_prime_UTR 30.0%
GTTTATACAAAACCAGATTC+AGG - chr4_4:50543607-50543626 None:intergenic 30.0%
! TTTTTCCGCTAATAGTTCGA+TGG + chr4_4:50542643-50542662 Msa0686220:exon 35.0%
! TTTTCCGCTAATAGTTCGAT+GGG + chr4_4:50542644-50542663 Msa0686220:exon 35.0%
GATTTCACCAAACTTAGAGA+AGG - chr4_4:50542744-50542763 None:intergenic 35.0%
! CTTTTGTTACTCACACCAAT+AGG + chr4_4:50542881-50542900 Msa0686220:intron 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_4:50542899-50542918 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_4:50542925-50542944 Msa0686220:intron 35.0%
! AAGATCGATCGGTTTCTATT+CGG + chr4_4:50542936-50542955 Msa0686220:intron 35.0%
GTTATCAATGATCGTGAAAC+TGG + chr4_4:50543022-50543041 Msa0686220:CDS 35.0%
GATGCAATTGAAGAGATGAA+TGG + chr4_4:50543097-50543116 Msa0686220:CDS 35.0%
AATTGAAGAGATGAATGGTC+AGG + chr4_4:50543102-50543121 Msa0686220:CDS 35.0%
ATGAATGGTCAGGATATTGA+CGG + chr4_4:50543112-50543131 Msa0686220:CDS 35.0%
ACTGGAGGGATTAGAAAATT+AGG + chr4_4:50543464-50543483 Msa0686220:exon 35.0%
AAATTAGGTCTTCAGTTTGG+TGG + chr4_4:50543479-50543498 Msa0686220:three_prime_UTR 35.0%
TGTCTTTATTGTCCTGAATC+TGG + chr4_4:50543592-50543611 Msa0686220:three_prime_UTR 35.0%
! TTTTTCCGCTAATAGTTCGA+TGG + chr4_4:50542643-50542662 Msa0686220:exon 35.0%
! TTTTCCGCTAATAGTTCGAT+GGG + chr4_4:50542644-50542663 Msa0686220:exon 35.0%
GATTTCACCAAACTTAGAGA+AGG - chr4_4:50542744-50542763 None:intergenic 35.0%
! CTTTTGTTACTCACACCAAT+AGG + chr4_4:50542881-50542900 Msa0686220:intron 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_4:50542899-50542918 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_4:50542925-50542944 Msa0686220:intron 35.0%
! AAGATCGATCGGTTTCTATT+CGG + chr4_4:50542936-50542955 Msa0686220:intron 35.0%
GTTATCAATGATCGTGAAAC+TGG + chr4_4:50543022-50543041 Msa0686220:CDS 35.0%
GATGCAATTGAAGAGATGAA+TGG + chr4_4:50543097-50543116 Msa0686220:CDS 35.0%
AATTGAAGAGATGAATGGTC+AGG + chr4_4:50543102-50543121 Msa0686220:CDS 35.0%
ATGAATGGTCAGGATATTGA+CGG + chr4_4:50543112-50543131 Msa0686220:CDS 35.0%
ACTGGAGGGATTAGAAAATT+AGG + chr4_4:50543464-50543483 Msa0686220:exon 35.0%
AAATTAGGTCTTCAGTTTGG+TGG + chr4_4:50543479-50543498 Msa0686220:three_prime_UTR 35.0%
TGTCTTTATTGTCCTGAATC+TGG + chr4_4:50543592-50543611 Msa0686220:three_prime_UTR 35.0%
AGAACCCATCGAACTATTAG+CGG - chr4_4:50542651-50542670 None:intergenic 40.0%
GTTCTTCTGATGTTGAGTAC+CGG + chr4_4:50542666-50542685 Msa0686220:CDS 40.0%
!! TGGTGAAATCACTGATTCCA+AGG + chr4_4:50542754-50542773 Msa0686220:CDS 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_4:50542929-50542948 None:intergenic 40.0%
!! GATCGGTTTCTATTCGGTTT+TGG + chr4_4:50542942-50542961 Msa0686220:intron 40.0%
! ACTGGAAGATCAAGAGGTTT+TGG + chr4_4:50543040-50543059 Msa0686220:CDS 40.0%
! ATTTTGAGCAGTGAACTCAG+AGG - chr4_4:50542607-50542626 None:intergenic 40.0%
AGAACCCATCGAACTATTAG+CGG - chr4_4:50542651-50542670 None:intergenic 40.0%
GTTCTTCTGATGTTGAGTAC+CGG + chr4_4:50542666-50542685 Msa0686220:CDS 40.0%
!! TGGTGAAATCACTGATTCCA+AGG + chr4_4:50542754-50542773 Msa0686220:CDS 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_4:50542929-50542948 None:intergenic 40.0%
!! GATCGGTTTCTATTCGGTTT+TGG + chr4_4:50542942-50542961 Msa0686220:intron 40.0%
! ACTGGAAGATCAAGAGGTTT+TGG + chr4_4:50543040-50543059 Msa0686220:CDS 40.0%
GAGCAAGCCTTCTCTAAGTT+TGG + chr4_4:50542734-50542753 Msa0686220:CDS 45.0%
AATCCGAATCAGAATCCGCA+CGG - chr4_4:50542781-50542800 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_4:50543034-50543053 Msa0686220:CDS 45.0%
GAGCAAGCCTTCTCTAAGTT+TGG + chr4_4:50542734-50542753 Msa0686220:CDS 45.0%
AATCCGAATCAGAATCCGCA+CGG - chr4_4:50542781-50542800 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_4:50543034-50543053 Msa0686220:CDS 45.0%
TTGCTCCAGAGCTTGGCTAT+CGG - chr4_4:50542720-50542739 None:intergenic 50.0%
ATGGTCAGGATATTGACGGC+AGG + chr4_4:50543116-50543135 Msa0686220:CDS 50.0%
GTGAATGAAGCTCAGTCACG+TGG + chr4_4:50543148-50543167 Msa0686220:CDS 50.0%
! TATGGTGGAGGTGGTGGTTA+TGG + chr4_4:50543208-50543227 Msa0686220:CDS 50.0%
!! GATGGTGGATACAGCAGAAG+TGG + chr4_4:50543271-50543290 Msa0686220:CDS 50.0%
! TGGTTATGGTGGAGGTGGTA+GGG + chr4_4:50543348-50543367 Msa0686220:CDS 50.0%
!! TGATAGCGGTGGAAACTGGA+GGG + chr4_4:50543450-50543469 Msa0686220:CDS 50.0%
TTGCTCCAGAGCTTGGCTAT+CGG - chr4_4:50542720-50542739 None:intergenic 50.0%
ATGGTCAGGATATTGACGGC+AGG + chr4_4:50543116-50543135 Msa0686220:CDS 50.0%
GTGAATGAAGCTCAGTCACG+TGG + chr4_4:50543148-50543167 Msa0686220:CDS 50.0%
! TATGGTGGAGGTGGTGGTTA+TGG + chr4_4:50543208-50543227 Msa0686220:CDS 50.0%
!! GATGGTGGATACAGCAGAAG+TGG + chr4_4:50543271-50543290 Msa0686220:CDS 50.0%
! TGGTTATGGTGGAGGTGGTA+GGG + chr4_4:50543348-50543367 Msa0686220:CDS 50.0%
!! TGATAGCGGTGGAAACTGGA+GGG + chr4_4:50543450-50543469 Msa0686220:CDS 50.0%
!! GTTGAGTACCGGTGCTTCGT+CGG + chr4_4:50542677-50542696 Msa0686220:CDS 55.0%
! CTTCGTCGGTGGTCTTGCTT+GGG + chr4_4:50542691-50542710 Msa0686220:CDS 55.0%
! AGAAGGCTTGCTCCAGAGCT+TGG - chr4_4:50542727-50542746 None:intergenic 55.0%
!! CACTGATTCCAAGGTCCGTG+CGG + chr4_4:50542763-50542782 Msa0686220:intron 55.0%
ATCAGAATCCGCACGGACCT+TGG - chr4_4:50542774-50542793 None:intergenic 55.0%
GGTCCGTGCGGATTCTGATT+CGG + chr4_4:50542775-50542794 Msa0686220:intron 55.0%
GGTGGATACAGCAGAAGTGG+TGG + chr4_4:50543274-50543293 Msa0686220:CDS 55.0%
GGATACAGCAGAAGTGGTGG+TGG + chr4_4:50543277-50543296 Msa0686220:CDS 55.0%
!! TACAGCAGAAGTGGTGGTGG+TGG + chr4_4:50543280-50543299 Msa0686220:CDS 55.0%
!! AGTGGTGGTGGTGGAGGATA+TGG + chr4_4:50543289-50543308 Msa0686220:CDS 55.0%
CACCACCTCCACCATATCCA+CGG - chr4_4:50543243-50543262 None:intergenic 55.0%
! GTGGTTATGGTGGAGGTGGT+AGG + chr4_4:50543347-50543366 Msa0686220:CDS 55.0%
CACCTCCACCACCATATCCA+CGG - chr4_4:50543375-50543394 None:intergenic 55.0%
!! GTGCTGATAGCGGTGGAAAC+TGG + chr4_4:50543446-50543465 Msa0686220:CDS 55.0%
!! CTGATAGCGGTGGAAACTGG+AGG + chr4_4:50543449-50543468 Msa0686220:CDS 55.0%
!! GTTGAGTACCGGTGCTTCGT+CGG + chr4_4:50542677-50542696 Msa0686220:CDS 55.0%
! CTTCGTCGGTGGTCTTGCTT+GGG + chr4_4:50542691-50542710 Msa0686220:CDS 55.0%
! AGAAGGCTTGCTCCAGAGCT+TGG - chr4_4:50542727-50542746 None:intergenic 55.0%
!! CACTGATTCCAAGGTCCGTG+CGG + chr4_4:50542763-50542782 Msa0686220:intron 55.0%
ATCAGAATCCGCACGGACCT+TGG - chr4_4:50542774-50542793 None:intergenic 55.0%
GGTCCGTGCGGATTCTGATT+CGG + chr4_4:50542775-50542794 Msa0686220:intron 55.0%
GGTGGATACAGCAGAAGTGG+TGG + chr4_4:50543274-50543293 Msa0686220:CDS 55.0%
GGATACAGCAGAAGTGGTGG+TGG + chr4_4:50543277-50543296 Msa0686220:CDS 55.0%
!! TACAGCAGAAGTGGTGGTGG+TGG + chr4_4:50543280-50543299 Msa0686220:CDS 55.0%
!! AGTGGTGGTGGTGGAGGATA+TGG + chr4_4:50543289-50543308 Msa0686220:CDS 55.0%
CACCACCTCCACCATATCCA+CGG - chr4_4:50543243-50543262 None:intergenic 55.0%
! GTGGTTATGGTGGAGGTGGT+AGG + chr4_4:50543347-50543366 Msa0686220:CDS 55.0%
CACCTCCACCACCATATCCA+CGG - chr4_4:50543375-50543394 None:intergenic 55.0%
!! GTGCTGATAGCGGTGGAAAC+TGG + chr4_4:50543446-50543465 Msa0686220:CDS 55.0%
!! CTGATAGCGGTGGAAACTGG+AGG + chr4_4:50543449-50543468 Msa0686220:CDS 55.0%
CAAGACCACCGACGAAGCAC+CGG - chr4_4:50542688-50542707 None:intergenic 60.0%
! GCTTCGTCGGTGGTCTTGCT+TGG + chr4_4:50542690-50542709 Msa0686220:CDS 60.0%
CTCCAGAGCTTGGCTATCGG+TGG - chr4_4:50542717-50542736 None:intergenic 60.0%
GAAGCTCAGTCACGTGGCAG+TGG + chr4_4:50543154-50543173 Msa0686220:CDS 60.0%
! AGTGGTGGAGGAGGTAGAGG+TGG + chr4_4:50543172-50543191 Msa0686220:CDS 60.0%
! GGTGGAGGTGGTGGTTATGG+AGG + chr4_4:50543211-50543230 Msa0686220:CDS 60.0%
! GGAGGTGGTGGTTATGGAGG+TGG + chr4_4:50543214-50543233 Msa0686220:CDS 60.0%
! GGTGGTGGTTATGGAGGTGG+CGG + chr4_4:50543217-50543236 Msa0686220:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + chr4_4:50543238-50543257 Msa0686220:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_4:50543241-50543260 Msa0686220:CDS 60.0%
!! AGCAGAAGTGGTGGTGGTGG+AGG + chr4_4:50543283-50543302 Msa0686220:CDS 60.0%
!! GGTGGTGGTGGAGGATATGG+TGG + chr4_4:50543292-50543311 Msa0686220:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+AGG + chr4_4:50543295-50543314 Msa0686220:CDS 60.0%
! GGTGGAGGATATGGTGGAGG+TGG + chr4_4:50543298-50543317 Msa0686220:CDS 60.0%
! TATGGTGGAGGTGGTGACCG+TGG + chr4_4:50543307-50543326 Msa0686220:CDS 60.0%
GGAGGTGGTGACCGTGGATA+TGG + chr4_4:50543313-50543332 Msa0686220:CDS 60.0%
GGTGGTGACCGTGGATATGG+TGG + chr4_4:50543316-50543335 Msa0686220:CDS 60.0%
GGTGACCGTGGATATGGTGG+AGG + chr4_4:50543319-50543338 Msa0686220:CDS 60.0%
! GACCGTGGATATGGTGGAGG+TGG + chr4_4:50543322-50543341 Msa0686220:CDS 60.0%
! CGTGGATATGGTGGAGGTGG+TGG + chr4_4:50543325-50543344 Msa0686220:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG + chr4_4:50543244-50543263 Msa0686220:CDS 60.0%
!! GGTGGAGGTGGTGGTGGTTA+TGG + chr4_4:50543334-50543353 Msa0686220:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_4:50543337-50543356 Msa0686220:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+AGG + chr4_4:50543340-50543359 Msa0686220:CDS 60.0%
! GGTGGTGGTTATGGTGGAGG+TGG + chr4_4:50543343-50543362 Msa0686220:CDS 60.0%
GGTGGTAGGGACCGTGGATA+TGG + chr4_4:50543361-50543380 Msa0686220:CDS 60.0%
GGTAGGGACCGTGGATATGG+TGG + chr4_4:50543364-50543383 Msa0686220:CDS 60.0%
AGGGACCGTGGATATGGTGG+TGG + chr4_4:50543367-50543386 Msa0686220:CDS 60.0%
!! GACCGTGGATATGGTGGTGG+AGG + chr4_4:50543370-50543389 Msa0686220:CDS 60.0%
!! CGTGGATATGGTGGTGGAGG+TGG + chr4_4:50543373-50543392 Msa0686220:CDS 60.0%
GGAGGTGGTGACCGTGGTTA+TGG + chr4_4:50543388-50543407 Msa0686220:CDS 60.0%
GGTGGTGACCGTGGTTATGG+TGG + chr4_4:50543391-50543410 Msa0686220:CDS 60.0%
GGTGACCGTGGTTATGGTGG+TGG + chr4_4:50543394-50543413 Msa0686220:CDS 60.0%
!! GACCGTGGTTATGGTGGTGG+TGG + chr4_4:50543397-50543416 Msa0686220:CDS 60.0%
CGCCACCACCACCATAACCA+CGG - chr4_4:50543402-50543421 None:intergenic 60.0%
!! CGTGGTTATGGTGGTGGTGG+CGG + chr4_4:50543400-50543419 Msa0686220:CDS 60.0%
CAAGACCACCGACGAAGCAC+CGG - chr4_4:50542688-50542707 None:intergenic 60.0%
! GCTTCGTCGGTGGTCTTGCT+TGG + chr4_4:50542690-50542709 Msa0686220:CDS 60.0%
CTCCAGAGCTTGGCTATCGG+TGG - chr4_4:50542717-50542736 None:intergenic 60.0%
GAAGCTCAGTCACGTGGCAG+TGG + chr4_4:50543154-50543173 Msa0686220:CDS 60.0%
! AGTGGTGGAGGAGGTAGAGG+TGG + chr4_4:50543172-50543191 Msa0686220:CDS 60.0%
! GGTGGAGGTGGTGGTTATGG+AGG + chr4_4:50543211-50543230 Msa0686220:CDS 60.0%
! GGAGGTGGTGGTTATGGAGG+TGG + chr4_4:50543214-50543233 Msa0686220:CDS 60.0%
! GGTGGTGGTTATGGAGGTGG+CGG + chr4_4:50543217-50543236 Msa0686220:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + chr4_4:50543238-50543257 Msa0686220:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_4:50543241-50543260 Msa0686220:CDS 60.0%
!! AGCAGAAGTGGTGGTGGTGG+AGG + chr4_4:50543283-50543302 Msa0686220:CDS 60.0%
!! GGTGGTGGTGGAGGATATGG+TGG + chr4_4:50543292-50543311 Msa0686220:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+AGG + chr4_4:50543295-50543314 Msa0686220:CDS 60.0%
! GGTGGAGGATATGGTGGAGG+TGG + chr4_4:50543298-50543317 Msa0686220:CDS 60.0%
! TATGGTGGAGGTGGTGACCG+TGG + chr4_4:50543307-50543326 Msa0686220:CDS 60.0%
GGAGGTGGTGACCGTGGATA+TGG + chr4_4:50543313-50543332 Msa0686220:CDS 60.0%
GGTGGTGACCGTGGATATGG+TGG + chr4_4:50543316-50543335 Msa0686220:CDS 60.0%
GGTGACCGTGGATATGGTGG+AGG + chr4_4:50543319-50543338 Msa0686220:CDS 60.0%
! GACCGTGGATATGGTGGAGG+TGG + chr4_4:50543322-50543341 Msa0686220:CDS 60.0%
! CGTGGATATGGTGGAGGTGG+TGG + chr4_4:50543325-50543344 Msa0686220:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG + chr4_4:50543244-50543263 Msa0686220:CDS 60.0%
!! GGTGGAGGTGGTGGTGGTTA+TGG + chr4_4:50543334-50543353 Msa0686220:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_4:50543337-50543356 Msa0686220:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+AGG + chr4_4:50543340-50543359 Msa0686220:CDS 60.0%
! GGTGGTGGTTATGGTGGAGG+TGG + chr4_4:50543343-50543362 Msa0686220:CDS 60.0%
GGTGGTAGGGACCGTGGATA+TGG + chr4_4:50543361-50543380 Msa0686220:CDS 60.0%
GGTAGGGACCGTGGATATGG+TGG + chr4_4:50543364-50543383 Msa0686220:CDS 60.0%
AGGGACCGTGGATATGGTGG+TGG + chr4_4:50543367-50543386 Msa0686220:CDS 60.0%
!! GACCGTGGATATGGTGGTGG+AGG + chr4_4:50543370-50543389 Msa0686220:CDS 60.0%
!! CGTGGATATGGTGGTGGAGG+TGG + chr4_4:50543373-50543392 Msa0686220:CDS 60.0%
GGAGGTGGTGACCGTGGTTA+TGG + chr4_4:50543388-50543407 Msa0686220:CDS 60.0%
GGTGGTGACCGTGGTTATGG+TGG + chr4_4:50543391-50543410 Msa0686220:CDS 60.0%
GGTGACCGTGGTTATGGTGG+TGG + chr4_4:50543394-50543413 Msa0686220:CDS 60.0%
!! GACCGTGGTTATGGTGGTGG+TGG + chr4_4:50543397-50543416 Msa0686220:CDS 60.0%
CGCCACCACCACCATAACCA+CGG - chr4_4:50543402-50543421 None:intergenic 60.0%
!! CGTGGTTATGGTGGTGGTGG+CGG + chr4_4:50543400-50543419 Msa0686220:CDS 60.0%
!! GAGTACCGGTGCTTCGTCGG+TGG + chr4_4:50542680-50542699 Msa0686220:CDS 65.0%
GGCCACCGATAGCCAAGCTC+TGG + chr4_4:50542712-50542731 Msa0686220:CDS 65.0%
GCTCAGTCACGTGGCAGTGG+TGG + chr4_4:50543157-50543176 Msa0686220:CDS 65.0%
CAGTCACGTGGCAGTGGTGG+AGG + chr4_4:50543160-50543179 Msa0686220:CDS 65.0%
! TCACGTGGCAGTGGTGGAGG+AGG + chr4_4:50543163-50543182 Msa0686220:CDS 65.0%
! GGCAGTGGTGGAGGAGGTAG+AGG + chr4_4:50543169-50543188 Msa0686220:CDS 65.0%
GGTGGAGGAGGTAGAGGTGG+CGG + chr4_4:50543175-50543194 Msa0686220:CDS 65.0%
! GGTGGGTATGGTGGAGGTGG+TGG + chr4_4:50543202-50543221 Msa0686220:CDS 65.0%
! GGTGGTTATGGAGGTGGCGG+TGG + chr4_4:50543220-50543239 Msa0686220:CDS 65.0%
! GGTTATGGAGGTGGCGGTGG+TGG + chr4_4:50543223-50543242 Msa0686220:CDS 65.0%
!! GGAGGTGGCGGTGGTGGATA+TGG + chr4_4:50543229-50543248 Msa0686220:CDS 65.0%
!! GGTGGCGGTGGTGGATATGG+TGG + chr4_4:50543232-50543251 Msa0686220:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+AGG + chr4_4:50543235-50543254 Msa0686220:CDS 65.0%
! GGAGGTGGTGGTCGTCGTGA+TGG + chr4_4:50543253-50543272 Msa0686220:CDS 65.0%
! GGTGGTGGTCGTCGTGATGG+TGG + chr4_4:50543256-50543275 Msa0686220:CDS 65.0%
GGCGGTGATCGCTACTCTCG+CGG + chr4_4:50543418-50543437 Msa0686220:CDS 65.0%
GGTGATCGCTACTCTCGCGG+TGG + chr4_4:50543421-50543440 Msa0686220:CDS 65.0%
GATCGCTACTCTCGCGGTGG+TGG + chr4_4:50543424-50543443 Msa0686220:CDS 65.0%
!! CGCGGTGGTGGTGCTGATAG+CGG + chr4_4:50543436-50543455 Msa0686220:CDS 65.0%
!! GGTGGTGGTGCTGATAGCGG+TGG + chr4_4:50543439-50543458 Msa0686220:CDS 65.0%
!! GAGTACCGGTGCTTCGTCGG+TGG + chr4_4:50542680-50542699 Msa0686220:CDS 65.0%
GGCCACCGATAGCCAAGCTC+TGG + chr4_4:50542712-50542731 Msa0686220:CDS 65.0%
GCTCAGTCACGTGGCAGTGG+TGG + chr4_4:50543157-50543176 Msa0686220:CDS 65.0%
CAGTCACGTGGCAGTGGTGG+AGG + chr4_4:50543160-50543179 Msa0686220:CDS 65.0%
! TCACGTGGCAGTGGTGGAGG+AGG + chr4_4:50543163-50543182 Msa0686220:CDS 65.0%
! GGCAGTGGTGGAGGAGGTAG+AGG + chr4_4:50543169-50543188 Msa0686220:CDS 65.0%
GGTGGAGGAGGTAGAGGTGG+CGG + chr4_4:50543175-50543194 Msa0686220:CDS 65.0%
! GGTGGGTATGGTGGAGGTGG+TGG + chr4_4:50543202-50543221 Msa0686220:CDS 65.0%
! GGTGGTTATGGAGGTGGCGG+TGG + chr4_4:50543220-50543239 Msa0686220:CDS 65.0%
! GGTTATGGAGGTGGCGGTGG+TGG + chr4_4:50543223-50543242 Msa0686220:CDS 65.0%
!! GGAGGTGGCGGTGGTGGATA+TGG + chr4_4:50543229-50543248 Msa0686220:CDS 65.0%
!! GGTGGCGGTGGTGGATATGG+TGG + chr4_4:50543232-50543251 Msa0686220:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+AGG + chr4_4:50543235-50543254 Msa0686220:CDS 65.0%
! GGAGGTGGTGGTCGTCGTGA+TGG + chr4_4:50543253-50543272 Msa0686220:CDS 65.0%
! GGTGGTGGTCGTCGTGATGG+TGG + chr4_4:50543256-50543275 Msa0686220:CDS 65.0%
GGCGGTGATCGCTACTCTCG+CGG + chr4_4:50543418-50543437 Msa0686220:CDS 65.0%
GGTGATCGCTACTCTCGCGG+TGG + chr4_4:50543421-50543440 Msa0686220:CDS 65.0%
GATCGCTACTCTCGCGGTGG+TGG + chr4_4:50543424-50543443 Msa0686220:CDS 65.0%
!! CGCGGTGGTGGTGCTGATAG+CGG + chr4_4:50543436-50543455 Msa0686220:CDS 65.0%
!! GGTGGTGGTGCTGATAGCGG+TGG + chr4_4:50543439-50543458 Msa0686220:CDS 65.0%
! GGAGGAGGTAGAGGTGGCGG+CGG + chr4_4:50543178-50543197 Msa0686220:CDS 70.0%
! GGCGGTGGGTATGGTGGAGG+TGG + chr4_4:50543199-50543218 Msa0686220:CDS 70.0%
GGTGGAGGTGGTAGGGACCG+TGG + chr4_4:50543355-50543374 Msa0686220:CDS 70.0%
!! GGTGGTGGAGGTGGTGACCG+TGG + chr4_4:50543382-50543401 Msa0686220:CDS 70.0%
! GGAGGAGGTAGAGGTGGCGG+CGG + chr4_4:50543178-50543197 Msa0686220:CDS 70.0%
! GGCGGTGGGTATGGTGGAGG+TGG + chr4_4:50543199-50543218 Msa0686220:CDS 70.0%
GGTGGAGGTGGTAGGGACCG+TGG + chr4_4:50543355-50543374 Msa0686220:CDS 70.0%
!! GGTGGTGGAGGTGGTGACCG+TGG + chr4_4:50543382-50543401 Msa0686220:CDS 70.0%
! GGAGGTAGAGGTGGCGGCGG+CGG + chr4_4:50543181-50543200 Msa0686220:CDS 75.0%
! GTAGAGGTGGCGGCGGCGGT+GGG + chr4_4:50543185-50543204 Msa0686220:CDS 75.0%
GGCGGCGGTGGGTATGGTGG+AGG + chr4_4:50543196-50543215 Msa0686220:CDS 75.0%
! GGAGGTAGAGGTGGCGGCGG+CGG + chr4_4:50543181-50543200 Msa0686220:CDS 75.0%
! GTAGAGGTGGCGGCGGCGGT+GGG + chr4_4:50543185-50543204 Msa0686220:CDS 75.0%
GGCGGCGGTGGGTATGGTGG+AGG + chr4_4:50543196-50543215 Msa0686220:CDS 75.0%
!! GGTAGAGGTGGCGGCGGCGG+TGG + chr4_4:50543184-50543203 Msa0686220:CDS 80.0%
!! GGTGGCGGCGGCGGTGGGTA+TGG + chr4_4:50543190-50543209 Msa0686220:CDS 80.0%
! GGCGGCGGCGGTGGGTATGG+TGG + chr4_4:50543193-50543212 Msa0686220:CDS 80.0%
!! GGTAGAGGTGGCGGCGGCGG+TGG + chr4_4:50543184-50543203 Msa0686220:CDS 80.0%
!! GGTGGCGGCGGCGGTGGGTA+TGG + chr4_4:50543190-50543209 Msa0686220:CDS 80.0%
! GGCGGCGGCGGTGGGTATGG+TGG + chr4_4:50543193-50543212 Msa0686220:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50542606 50543676 50542606 ID=Msa0686220;Name=Msa0686220
chr4_4 mRNA 50542606 50543676 50542606 ID=Msa0686220-mRNA-1;Parent=Msa0686220;Name=Msa0686220-mRNA-1;_AED=0.08;_eAED=0.08;_QI=56|1|1|1|1|1|2|199|189
chr4_4 exon 50542606 50542775 50542606 ID=Msa0686220-mRNA-1:exon:12944;Parent=Msa0686220-mRNA-1
chr4_4 exon 50543022 50543676 50543022 ID=Msa0686220-mRNA-1:exon:12945;Parent=Msa0686220-mRNA-1
chr4_4 five_prime_UTR 50542606 50542661 50542606 ID=Msa0686220-mRNA-1:five_prime_utr;Parent=Msa0686220-mRNA-1
chr4_4 CDS 50542662 50542775 50542662 ID=Msa0686220-mRNA-1:cds;Parent=Msa0686220-mRNA-1
chr4_4 CDS 50543022 50543477 50543022 ID=Msa0686220-mRNA-1:cds;Parent=Msa0686220-mRNA-1
chr4_4 three_prime_UTR 50543478 50543676 50543478 ID=Msa0686220-mRNA-1:three_prime_utr;Parent=Msa0686220-mRNA-1
Gene Sequence

>Msa0686220

ATGGGTTCTTCTGATGTTGAGTACCGGTGCTTCGTCGGTGGTCTTGCTTGGGCCACCGATAGCCAAGCTCTGGAGCAAGCCTTCTCTAAGTTTGGTGAAATCACTGATTCCAAGGTTATCAATGATCGTGAAACTGGAAGATCAAGAGGTTTTGGATTTGTGACATTCGCTGATGAGAAATCGATGAGAGATGCAATTGAAGAGATGAATGGTCAGGATATTGACGGCAGGAATATTACTGTGAATGAAGCTCAGTCACGTGGCAGTGGTGGAGGAGGTAGAGGTGGCGGCGGCGGTGGGTATGGTGGAGGTGGTGGTTATGGAGGTGGCGGTGGTGGATATGGTGGAGGTGGTGGTCGTCGTGATGGTGGATACAGCAGAAGTGGTGGTGGTGGAGGATATGGTGGAGGTGGTGACCGTGGATATGGTGGAGGTGGTGGTGGTTATGGTGGAGGTGGTAGGGACCGTGGATATGGTGGTGGAGGTGGTGACCGTGGTTATGGTGGTGGTGGCGGTGATCGCTACTCTCGCGGTGGTGGTGCTGATAGCGGTGGAAACTGGAGGGATTAG
Protein sequence

>Msa0686220

MGSSDVEYRCFVGGLAWATDSQALEQAFSKFGEITDSKVINDRETGRSRGFGFVTFADEKSMRDAIEEMNGQDIDGRNITVNEAQSRGSGGGGRGGGGGGYGGGGGYGGGGGGYGGGGGRRDGGYSRSGGGGGYGGGGDRGYGGGGGGYGGGGRDRGYGGGGGDRGYGGGGGDRYSRGGGADSGGNWRD*