AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1212270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212270 MtrunA17_Chr4g0061021 97.778 585 13 0 1 585 1 585 0.0 1202
Msa1212270 MtrunA17_Chr3g0091641 63.484 597 157 7 19 585 10 575 0.0 730
Msa1212270 MtrunA17_Chr4g0030271 44.531 128 71 0 27 154 83 210 8.54e-32 131
Msa1212270 MtrunA17_Chr4g0030271 70.833 48 14 0 501 548 717 764 3.32e-16 82.4
Msa1212270 MtrunA17_Chr3g0127941 44.186 129 72 0 27 155 50 178 4.03e-30 125
Msa1212270 MtrunA17_Chr3g0127941 73.333 45 12 0 505 549 630 674 4.15e-14 75.5
Msa1212270 MtrunA17_Chr7g0276361 33.816 207 123 5 27 229 8 204 4.77e-28 119
Msa1212270 MtrunA17_Chr7g0276361 64.000 50 18 0 501 550 537 586 1.33e-14 77.0
Msa1212270 MtrunA17_Chr8g0345901 39.844 128 77 0 27 154 30 157 2.36e-27 117
Msa1212270 MtrunA17_Chr8g0345901 71.111 45 13 0 504 548 583 627 4.42e-13 72.0
Msa1212270 MtrunA17_Chr1g0181811 40.625 128 76 0 27 154 79 206 3.61e-26 113
Msa1212270 MtrunA17_Chr1g0181811 68.085 47 12 1 504 547 690 736 1.02e-12 71.2
Msa1212270 MtrunA17_Chr3g0123131 35.772 123 76 1 33 155 17 136 8.13e-20 93.2
Msa1212270 MtrunA17_Chr3g0134481 36.090 133 80 2 25 155 2 131 4.76e-19 85.5
Msa1212270 MtrunA17_Chr2g0294911 36.290 124 76 1 33 156 33 153 5.98e-19 90.5
Msa1212270 MtrunA17_Chr2g0304841 33.571 140 88 2 19 156 6 142 6.06e-19 90.9
Msa1212270 MtrunA17_Chr3g0091661 60.000 70 24 2 46 115 27 92 8.24e-19 82.0
Msa1212270 MtrunA17_Chr4g0067981 35.036 137 83 3 22 156 23 155 9.27e-19 90.1
Msa1212270 MtrunA17_Chr1g0161431 33.607 122 78 1 33 154 15 133 1.03e-18 90.1
Msa1212270 MtrunA17_Chr3g0137101 33.582 134 84 2 23 156 4 132 6.24e-18 87.4
Msa1212270 MtrunA17_Chr4g0074411 33.333 123 79 1 33 155 22 141 8.17e-18 87.0
Msa1212270 MtrunA17_Chr8g0376881 34.146 123 78 1 33 155 29 148 2.89e-17 85.1
Msa1212270 MtrunA17_Chr7g0275401 31.496 127 84 1 30 156 6 129 6.34e-17 81.6
Msa1212270 MtrunA17_Chr1g0149841 31.452 124 82 1 33 156 9 129 9.90e-17 83.2
Msa1212270 MtrunA17_Chr1g0149851 31.452 124 82 1 33 156 9 129 4.46e-16 81.3
Msa1212270 MtrunA17_Chr7g0275891 30.435 138 92 2 33 169 9 143 1.18e-15 78.6
Msa1212270 MtrunA17_Chr4g0074401 26.630 184 105 3 33 211 21 179 8.71e-15 77.4
Msa1212270 MtrunA17_Chr3g0134461 30.400 125 83 2 33 156 12 133 5.71e-12 65.1
Msa1212270 MtrunA17_Chr5g0400241 29.134 127 84 3 26 151 10 131 5.79e-12 68.6
Msa1212270 MtrunA17_Chr4g0049741 64.444 45 16 0 501 545 104 148 1.63e-11 65.9
Msa1212270 MtrunA17_Chr7g0256551 25.676 148 92 1 26 155 2 149 4.21e-11 63.5
Msa1212270 MtrunA17_Chr5g0427661 52.459 61 27 1 505 563 135 195 5.90e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212270 AT5G61380.1 49.534 644 206 19 22 584 8 613 3.66e-179 520
Msa1212270 AT5G60100.7 45.902 122 66 0 33 154 64 185 7.11e-31 125
Msa1212270 AT5G60100.6 45.902 122 66 0 33 154 64 185 7.11e-31 125
Msa1212270 AT5G60100.4 45.902 122 66 0 33 154 64 185 7.11e-31 125
Msa1212270 AT5G60100.5 45.902 122 66 0 33 154 64 185 1.38e-30 125
Msa1212270 AT5G60100.5 60.606 66 19 2 489 547 419 484 3.59e-15 79.0
Msa1212270 AT5G60100.3 45.902 122 66 0 33 154 64 185 1.38e-30 125
Msa1212270 AT5G60100.3 60.606 66 19 2 489 547 419 484 3.59e-15 79.0
Msa1212270 AT5G60100.1 45.902 122 66 0 33 154 64 185 1.38e-30 125
Msa1212270 AT5G60100.1 60.606 66 19 2 489 547 419 484 3.59e-15 79.0
Msa1212270 AT5G24470.1 43.609 133 75 0 27 159 44 176 2.35e-30 125
Msa1212270 AT5G24470.1 66.667 45 15 0 504 548 508 552 2.04e-13 73.6
Msa1212270 AT5G60100.2 45.902 122 66 0 33 154 64 185 4.76e-30 124
Msa1212270 AT5G60100.2 60.606 66 19 2 489 547 446 511 4.98e-15 78.6
Msa1212270 AT2G46790.3 40.625 128 76 0 27 154 31 158 9.35e-30 122
Msa1212270 AT2G46790.1 40.625 128 76 0 27 154 31 158 1.12e-29 122
Msa1212270 AT2G46790.1 76.087 46 11 0 504 549 416 461 2.80e-16 82.0
Msa1212270 AT5G02810.1 42.969 128 73 0 27 154 72 199 3.87e-27 117
Msa1212270 AT5G02810.1 74.510 51 13 0 497 547 661 711 6.46e-17 85.1
Msa1212270 AT3G16857.2 35.714 126 78 1 31 156 35 157 1.04e-19 93.6
Msa1212270 AT3G16857.1 35.714 126 78 1 31 156 35 157 1.06e-19 93.6
Msa1212270 AT2G25180.1 34.146 123 78 1 33 155 17 136 6.02e-19 91.3
Msa1212270 AT4G31920.1 34.711 121 76 1 33 153 17 134 1.71e-18 89.4
Msa1212270 AT1G67710.1 34.959 123 77 1 33 155 11 130 8.01e-18 87.4
Msa1212270 AT2G46670.1 77.778 45 10 0 505 549 132 176 9.48e-18 82.0
Msa1212270 AT5G02810.2 74.510 51 13 0 497 547 517 567 4.33e-17 85.1
Msa1212270 AT2G46790.5 76.087 46 11 0 504 549 259 304 5.61e-17 82.4
Msa1212270 AT2G46790.4 76.087 46 11 0 504 549 259 304 5.61e-17 82.4
Msa1212270 AT2G46790.2 76.087 46 11 0 504 549 299 344 1.62e-16 81.6
Msa1212270 AT2G46790.2 63.415 41 15 0 114 154 1 41 6.25e-13 70.9
Msa1212270 AT3G16857.3 42.308 78 42 1 79 156 13 87 1.26e-14 77.4
Msa1212270 AT2G01760.1 32.000 125 83 1 31 155 9 131 3.46e-14 75.1
Msa1212270 AT5G58080.1 32.576 132 83 3 25 154 8 135 6.09e-14 75.5
Msa1212270 AT3G62670.1 31.008 129 83 3 27 155 36 158 1.03e-13 73.9
Msa1212270 AT2G01760.2 30.909 110 74 1 46 155 52 159 4.02e-11 65.9

Find 185 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATACTCATGGGAAAATA+TGG 0.142897 8_1:-72116610 None:intergenic
TCTTATTAATTGCTCTTATC+AGG 0.143425 8_1:+72113235 Msa1212270:CDS
GAACCATAACTTCCATTTCT+TGG 0.183017 8_1:-72116382 None:intergenic
CTCGGGATTATTGCTTCTTT+TGG 0.197660 8_1:-72115238 None:intergenic
ATGAAAATTGGAAAGGAAAA+TGG 0.215886 8_1:-72112856 None:intergenic
GCTTACTTATAATAGGAATT+TGG 0.224959 8_1:-72114367 None:intergenic
TATGTTTAATTGGAACTATT+TGG 0.230474 8_1:+72117277 Msa1212270:three_prime_UTR
GCCTCTGATCCTTGCTTTGA+TGG 0.277835 8_1:+72117036 Msa1212270:three_prime_UTR
GTTGCTTCGATAAGAAAATT+AGG 0.301363 8_1:+72116767 Msa1212270:CDS
TATTGAGTTGTGTGTAGTTT+AGG 0.302928 8_1:+72113041 Msa1212270:five_prime_UTR
GGTATTAATGTGGATCTAAA+TGG 0.303023 8_1:+72116859 Msa1212270:CDS
CAGAAAGGCGGCCTCGTGTT+AGG 0.304907 8_1:+72116812 Msa1212270:CDS
TGCTTTGACATAAGTGAAAA+AGG 0.328177 8_1:-72116071 None:intergenic
GACCTTATAAGACATGAAAA+TGG 0.329865 8_1:+72116202 Msa1212270:CDS
GTTACAAATAGTAGCTTTGA+TGG 0.334131 8_1:+72115829 Msa1212270:CDS
GAAAATGGGGTTTGCGAGAT+TGG 0.335676 8_1:-72112841 None:intergenic
TTTATACATCTAATTAGTTA+AGG 0.346044 8_1:+72112975 Msa1212270:five_prime_UTR
GGACCCAAAAGGATGAAAAT+TGG 0.349126 8_1:-72112868 None:intergenic
TGAAAATTGGAAAGGAAAAT+GGG 0.351414 8_1:-72112855 None:intergenic
AAATTTCCGCCTGTTCTTGT+TGG 0.352269 8_1:-72115275 None:intergenic
TTAGTGCAGGGAAGAAATAA+TGG 0.358556 8_1:-72116993 None:intergenic
CAATGTCCACCTCATGCAAC+TGG 0.364014 8_1:+72116574 Msa1212270:CDS
TAACATTCACCTCTTCTTGT+TGG 0.368007 8_1:-72115302 None:intergenic
CTTCTTCGATCACTAATTCC+AGG 0.369829 8_1:-72115537 None:intergenic
CACTGCTGCTGCTGTTATTG+AGG 0.387890 8_1:+72115473 Msa1212270:CDS
TAACATGACTCTGAAGGTTC+TGG 0.390005 8_1:-72116516 None:intergenic
CTAATGAACTATTAAATTTG+TGG 0.394489 8_1:+72114574 Msa1212270:CDS
GCTCATCCATGGCCGTCATT+AGG 0.398082 8_1:+72116658 Msa1212270:CDS
CATCCAAGAAATGGAAGTTA+TGG 0.399698 8_1:+72116379 Msa1212270:CDS
AGTTGCATGAGGTGGACATT+GGG 0.404126 8_1:-72116572 None:intergenic
AAGAAGAGTGAACTCAGAAT+TGG 0.405640 8_1:+72116037 Msa1212270:CDS
AACTAAAGCTTACTTATAAT+AGG 0.406920 8_1:-72114374 None:intergenic
AGTTCAGACAAACTCTTGTT+TGG 0.407505 8_1:+72117152 Msa1212270:three_prime_UTR
TGTGTGTAGTTTAGGCTAAA+GGG 0.408267 8_1:+72113049 Msa1212270:five_prime_UTR
AAAATGGGGTTTGCGAGATT+GGG 0.411075 8_1:-72112840 None:intergenic
TCGGGGATACTATTGCTTCT+AGG 0.411593 8_1:-72116259 None:intergenic
ATTCTGAGTTCACTCTTCTT+CGG 0.411743 8_1:-72116034 None:intergenic
TTATACATCTAATTAGTTAA+GGG 0.414705 8_1:+72112976 Msa1212270:five_prime_UTR
CTGTTTGTATTGGCATCACT+AGG 0.416022 8_1:-72115180 None:intergenic
GTGTTAGGGGACAGTTTGTC+AGG 0.418641 8_1:+72116827 Msa1212270:CDS
CTGAGGGTGCCTAATGGAAT+TGG 0.421359 8_1:-72117115 None:intergenic
AATGCTGATCCAATTCCATT+AGG 0.422628 8_1:+72117106 Msa1212270:three_prime_UTR
AGAAAGGCGGCCTCGTGTTA+GGG 0.423017 8_1:+72116813 Msa1212270:CDS
TTGTGTGTAGTTTAGGCTAA+AGG 0.424289 8_1:+72113048 Msa1212270:five_prime_UTR
ATTGGGGAAGTTGATTGTAC+TGG 0.429425 8_1:-72116555 None:intergenic
CTCTCAACAGAGAAAGAATC+GGG 0.433841 8_1:-72116277 None:intergenic
TCATTGATAGGAGTAAAGTT+AGG 0.434757 8_1:+72113162 Msa1212270:CDS
TAGTGCAGGGAAGAAATAAT+GGG 0.435052 8_1:-72116992 None:intergenic
GAAGGTGGCACTGTTTGTAT+TGG 0.449438 8_1:-72115190 None:intergenic
TGACCTTCTCAAGCATCCTT+AGG 0.451954 8_1:-72116955 None:intergenic
TAGGTAAGTCATCTTGTGAA+TGG 0.453633 8_1:-72116240 None:intergenic
GGGGTGGTTCATCCAAGAAA+TGG 0.454607 8_1:+72116370 Msa1212270:CDS
GTGTACCCCACCTGCATCAA+TGG 0.457279 8_1:+72116303 Msa1212270:CDS
TGGTTCTGAGCTCAACCAAT+CGG 0.464264 8_1:+72116399 Msa1212270:CDS
AATAATAGCAAGAGTGGTGA+TGG 0.468780 8_1:+72113137 Msa1212270:CDS
ATTAGTGATCGAAGAAGAAC+AGG 0.469944 8_1:+72115543 Msa1212270:CDS
TATTCTGATACATGAACATA+TGG 0.472895 8_1:-72115501 None:intergenic
AACAGTAAAGAGGTTAATTG+TGG 0.476504 8_1:+72113110 Msa1212270:CDS
AATAGTTCATTAGTACGTAA+AGG 0.484282 8_1:-72114564 None:intergenic
GCAACTCCTGGCATATAATA+CGG 0.486587 8_1:-72116445 None:intergenic
AATTTCAATCCAACAAGAAC+AGG 0.489300 8_1:+72115266 Msa1212270:CDS
TATTGATTTGAACAGTAAAG+AGG 0.489414 8_1:+72113100 Msa1212270:CDS
CACAGCAAAATAGTAATCAC+AGG 0.492222 8_1:+72116332 Msa1212270:CDS
CTAAAGGGGTTGATTTGAGA+TGG 0.493057 8_1:+72113064 Msa1212270:exon
TCTCTCAACAGAGAAAGAAT+CGG 0.495017 8_1:-72116278 None:intergenic
TAATTGGAACTATTTGGAGC+AGG 0.495661 8_1:+72117283 Msa1212270:three_prime_UTR
TCGTCATAGTCGGTGGAAGC+AGG 0.498204 8_1:-72116886 None:intergenic
TCACTAATTCCAGGTACATC+AGG 0.499237 8_1:-72115528 None:intergenic
AGAAGCAATAATCCCGAGGC+TGG 0.500098 8_1:+72115243 Msa1212270:CDS
CTTATTAATTGCTCTTATCA+GGG 0.502067 8_1:+72113236 Msa1212270:CDS
AAATACTCATGGGAAAATAT+GGG 0.502365 8_1:-72116609 None:intergenic
ATAATACGGATAAGCATGTT+GGG 0.502826 8_1:-72116431 None:intergenic
AGAAAATTAGGTACGTCAAT+CGG 0.504195 8_1:+72116779 Msa1212270:CDS
GTTGGGCAGGCATGCCCGAT+TGG 0.504294 8_1:-72116414 None:intergenic
TTGGATTGAAATTCCAGCCT+CGG 0.504832 8_1:-72115256 None:intergenic
TGGATTGAAATTCCAGCCTC+GGG 0.511665 8_1:-72115255 None:intergenic
GAATATCAGCCTGATGTACC+TGG 0.511952 8_1:+72115519 Msa1212270:CDS
GTGGTGATGGGTTCATTGAT+AGG 0.512716 8_1:+72113150 Msa1212270:CDS
GCACTAAAATTGTCCTATGC+AGG 0.513122 8_1:+72117009 Msa1212270:three_prime_UTR
CAGTTTGTCAGGAAGTTGAA+TGG 0.513514 8_1:+72116838 Msa1212270:CDS
TACGGATAAGCATGTTGGGC+AGG 0.516130 8_1:-72116427 None:intergenic
TATAATACGGATAAGCATGT+TGG 0.516804 8_1:-72116432 None:intergenic
CAATTCCATTAGGCACCCTC+AGG 0.522343 8_1:+72117116 Msa1212270:three_prime_UTR
TATTTGTTTACAGACCGGTC+AGG 0.523053 8_1:+72116627 Msa1212270:CDS
GGTTCTGAGCTCAACCAATC+GGG 0.529063 8_1:+72116400 Msa1212270:CDS
GTTGTCAAGTGCTTGAGGCT+CGG 0.530125 8_1:+72114519 Msa1212270:CDS
GCTTATCCGTATTATATGCC+AGG 0.531404 8_1:+72116439 Msa1212270:CDS
TGTCTGAACTCCACAGGATG+AGG 0.532533 8_1:-72117139 None:intergenic
GTCTGTGTCATCAGAGAAGA+AGG 0.532621 8_1:-72115208 None:intergenic
AAGACATGAAAATGGAGAAA+CGG 0.538385 8_1:+72116210 Msa1212270:CDS
CACCAGGGATTCCTCTCCTA+AGG 0.541039 8_1:+72116939 Msa1212270:CDS
CAGTTGCATGAGGTGGACAT+TGG 0.541645 8_1:-72116573 None:intergenic
ATGAGTATTTGTTTACAGAC+CGG 0.543051 8_1:+72116622 Msa1212270:CDS
GTGTGTAGTTTAGGCTAAAG+GGG 0.543328 8_1:+72113050 Msa1212270:five_prime_UTR
GAACTATTTGGAGCAGGCTG+TGG 0.545888 8_1:+72117289 Msa1212270:three_prime_UTR
CTCAATAACAGCAGCAGCAG+TGG 0.545949 8_1:-72115472 None:intergenic
ATAACATGATTAGCAACTCC+TGG 0.549528 8_1:-72116457 None:intergenic
AGTAAAGAGGTTAATTGTGG+TGG 0.552977 8_1:+72113113 Msa1212270:CDS
TGATTCCAAGAGTTCAGAAG+AGG 0.553393 8_1:+72113202 Msa1212270:CDS
CTCATGGGAAAATATGGGTA+GGG 0.554839 8_1:-72116604 None:intergenic
GAAAGGCGGCCTCGTGTTAG+GGG 0.555000 8_1:+72116814 Msa1212270:CDS
GGTCTGTAAACAAATACTCA+TGG 0.558038 8_1:-72116620 None:intergenic
TGTGGACGCACATGTGGAGA+AGG 0.558944 8_1:+72114592 Msa1212270:CDS
GTCTGTAAACAAATACTCAT+GGG 0.559531 8_1:-72116619 None:intergenic
TGTTTGTATTGGCATCACTA+GGG 0.561104 8_1:-72115179 None:intergenic
GTCATCCCAGTTGCATGAGG+TGG 0.561134 8_1:-72116580 None:intergenic
CAATTGTTGTCAAGTGCTTG+AGG 0.561695 8_1:+72114514 Msa1212270:CDS
TAAATTTGTGGACGCACATG+TGG 0.562177 8_1:+72114586 Msa1212270:CDS
GCAGAAGGTGAACCTCCTGC+TGG 0.564434 8_1:+72117223 Msa1212270:three_prime_UTR
ACTCATGGGAAAATATGGGT+AGG 0.564653 8_1:-72116605 None:intergenic
TTGGGGAAGTTGATTGTACT+GGG 0.566596 8_1:-72116554 None:intergenic
CTATTTGTAACTCTGTTGCA+CGG 0.566787 8_1:-72115817 None:intergenic
TCCTACTGAGCTCTTGCAGA+AGG 0.568787 8_1:+72117208 Msa1212270:three_prime_UTR
ATCCTTAGGAGAGGAATCCC+TGG 0.570852 8_1:-72116941 None:intergenic
ATCGGAAACAACTTGCAGAA+AGG 0.571293 8_1:+72116797 Msa1212270:CDS
CTCCACAGGATGAGGCCTGA+GGG 0.573154 8_1:-72117131 None:intergenic
TTCATCCTCATCGTCATAGT+CGG 0.573949 8_1:-72116896 None:intergenic
CAAAAGGATGAAAATTGGAA+AGG 0.579953 8_1:-72112863 None:intergenic
AACAACAATGAAGAGCAACT+TGG 0.581389 8_1:+72116093 Msa1212270:CDS
AGGAAGAAAACAATGACACC+AGG 0.582197 8_1:+72116923 Msa1212270:CDS
AGGTTTCTACAGCTCATCCA+TGG 0.583579 8_1:+72116647 Msa1212270:CDS
AGATGTTGAAGTACATAGCA+CGG 0.586911 8_1:+72114330 Msa1212270:CDS
ATCAAGCATGTTCTCAACAA+GGG 0.587610 8_1:+72116173 Msa1212270:CDS
CTTGGATGAACCACCCCTTG+TGG 0.587951 8_1:-72116364 None:intergenic
CTATGACGATGAGGATGAAG+AGG 0.588668 8_1:+72116900 Msa1212270:CDS
GAAAATTGGAAAGGAAAATG+GGG 0.590253 8_1:-72112854 None:intergenic
GATGTTGAAGTACATAGCAC+GGG 0.593466 8_1:+72114331 Msa1212270:CDS
ACCTTCTGCAAGAGCTCAGT+AGG 0.593616 8_1:-72117209 None:intergenic
GAAGTTGAATGGTATTAATG+TGG 0.594169 8_1:+72116849 Msa1212270:CDS
TCCATCAAAGCAAGGATCAG+AGG 0.597704 8_1:-72117037 None:intergenic
TCTCCTAAGGATGCTTGAGA+AGG 0.598049 8_1:+72116952 Msa1212270:exon
TGTGAAACCTCCATTGATGC+AGG 0.598440 8_1:-72116313 None:intergenic
AACCTCCATTGATGCAGGTG+GGG 0.598604 8_1:-72116308 None:intergenic
AGAGTTTGTCTGAACTCCAC+AGG 0.598864 8_1:-72117145 None:intergenic
ACTCCACAGGATGAGGCCTG+AGG 0.600756 8_1:-72117132 None:intergenic
CAAACTTCAGAAAAGTCGAT+AGG 0.608964 8_1:+72116701 Msa1212270:CDS
AATTTCAATCCAACAAGAAG+AGG 0.609391 8_1:+72115293 Msa1212270:CDS
ATCCTCATCGTCATAGTCGG+TGG 0.610990 8_1:-72116893 None:intergenic
GGTGTCATCCCAGTTGCATG+AGG 0.611782 8_1:-72116583 None:intergenic
GAAGAAATAATGGGACAAAG+AGG 0.618056 8_1:-72116983 None:intergenic
TTGATGGAAAGTTATTGTGT+CGG 0.618169 8_1:+72117052 Msa1212270:three_prime_UTR
AATGTCCACCTCATGCAACT+GGG 0.619079 8_1:+72116575 Msa1212270:CDS
AATAACTTTCCATCAAAGCA+AGG 0.619953 8_1:-72117045 None:intergenic
AGTGATGTCTGCACAAGATG+AGG 0.622148 8_1:+72114488 Msa1212270:intron
CGGTACATTACTAGTGAGGG+TGG 0.622559 8_1:+72116141 Msa1212270:CDS
AATCAAGCATGTTCTCAACA+AGG 0.623938 8_1:+72116172 Msa1212270:CDS
TTCTCAAGCATCCTTAGGAG+AGG 0.624497 8_1:-72116950 None:intergenic
TTCAATCCAACAAGAACAGG+CGG 0.626266 8_1:+72115269 Msa1212270:CDS
TCAAGCATGTTCTCAACAAG+GGG 0.626567 8_1:+72116174 Msa1212270:CDS
ATGAACTAACATGACTCTGA+AGG 0.628622 8_1:-72116522 None:intergenic
GGTGGAAATAATAGCAAGAG+TGG 0.630214 8_1:+72113131 Msa1212270:CDS
ATAATAGCAAGAGTGGTGAT+GGG 0.630687 8_1:+72113138 Msa1212270:CDS
GTTGCATGAGGTGGACATTG+GGG 0.632474 8_1:-72116571 None:intergenic
ATCAGAGGCAAGTCCTGCAT+AGG 0.635679 8_1:-72117022 None:intergenic
ATAGCGGTACATTACTAGTG+AGG 0.638404 8_1:+72116137 Msa1212270:CDS
CACAGGGAAGAACATCCACA+AGG 0.638565 8_1:+72116349 Msa1212270:CDS
ACTCATTGGACATAACCAGC+AGG 0.642043 8_1:-72117238 None:intergenic
GGAAACAACTTGCAGAAAGG+CGG 0.642044 8_1:+72116800 Msa1212270:CDS
GAAACCTCCATTGATGCAGG+TGG 0.645336 8_1:-72116310 None:intergenic
ATGAGCTGTAGAAACCTGAC+CGG 0.646047 8_1:-72116641 None:intergenic
GGAAGAACATCCACAAGGGG+TGG 0.646616 8_1:+72116354 Msa1212270:CDS
TGACGATGAGGATGAAGAGG+AGG 0.647407 8_1:+72116903 Msa1212270:CDS
GAACTTCCTAATGACGGCCA+TGG 0.652127 8_1:-72116664 None:intergenic
TACCCCACCTGCATCAATGG+AGG 0.653828 8_1:+72116306 Msa1212270:CDS
AAACCTCCATTGATGCAGGT+GGG 0.653891 8_1:-72116309 None:intergenic
AGTGATTGACGCATTGAATG+CGG 0.661141 8_1:+72114247 Msa1212270:CDS
ATTGACGCATTGAATGCGGA+AGG 0.662540 8_1:+72114251 Msa1212270:CDS
GAAGTTGAACTTCCTAATGA+CGG 0.668884 8_1:-72116670 None:intergenic
TAGCGGTACATTACTAGTGA+GGG 0.670900 8_1:+72116138 Msa1212270:CDS
TTACGTCTGTAAAATCTGCA+CGG 0.675024 8_1:+72114222 Msa1212270:CDS
CAGGGAAGAACATCCACAAG+GGG 0.677957 8_1:+72116351 Msa1212270:CDS
ATATATAGATATTATACTAG+CGG 0.684747 8_1:+72114277 Msa1212270:CDS
ACAGCAAAATAGTAATCACA+GGG 0.685208 8_1:+72116333 Msa1212270:CDS
CAAAAGAAGCAATAATCCCG+AGG 0.687400 8_1:+72115239 Msa1212270:CDS
TTCCACCGACTATGACGATG+AGG 0.690682 8_1:+72116891 Msa1212270:CDS
ACAGGGAAGAACATCCACAA+GGG 0.694566 8_1:+72116350 Msa1212270:CDS
GGAAGAAAACAATGACACCA+GGG 0.694692 8_1:+72116924 Msa1212270:CDS
CATGTGGAGAAGGAGACGCA+TGG 0.700199 8_1:+72114602 Msa1212270:CDS
ATTGCTCATTCTGACAATAG+CGG 0.712115 8_1:+72116121 Msa1212270:CDS
CATTGGACATAACCAGCAGG+AGG 0.715453 8_1:-72117235 None:intergenic
TGTGTCATCAGAGAAGAAGG+TGG 0.715634 8_1:-72115205 None:intergenic
ACAAACTGTCCCCTAACACG+AGG 0.728956 8_1:-72116823 None:intergenic
TTTGTAACTCTGTTGCACGG+CGG 0.729169 8_1:-72115814 None:intergenic
TCTCAACAGAGAAAGAATCG+GGG 0.738856 8_1:-72116276 None:intergenic
CAAGCATGTTCTCAACAAGG+GGG 0.755371 8_1:+72116175 Msa1212270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTAAAACTTTTAAACTCAT+TGG - chr8_1:72117255-72117274 None:intergenic 20.0%
!!! GTTTTACACTTATGTTTAAT+TGG + chr8_1:72117267-72117286 Msa1212270:three_prime_UTR 20.0%
!! TATGTTTAATTGGAACTATT+TGG + chr8_1:72117277-72117296 Msa1212270:three_prime_UTR 20.0%
!!! TGACAAAAAACTCATTTTTT+GGG - chr8_1:72117358-72117377 None:intergenic 20.0%
!!! TTGACAAAAAACTCATTTTT+TGG - chr8_1:72117359-72117378 None:intergenic 20.0%
AATAACTTTCCATCAAAGCA+AGG - chr8_1:72117048-72117067 None:intergenic 30.0%
TTGATGGAAAGTTATTGTGT+CGG + chr8_1:72117052-72117071 Msa1212270:three_prime_UTR 30.0%
!! TGGATCAGCATTTTAATCAA+AGG - chr8_1:72117098-72117117 None:intergenic 30.0%
!!! CATTTTTTGGGACAACTTAA+AGG - chr8_1:72117346-72117365 None:intergenic 30.0%
! GAAGAAATAATGGGACAAAG+AGG - chr8_1:72116986-72117005 None:intergenic 35.0%
TAGTGCAGGGAAGAAATAAT+GGG - chr8_1:72116995-72117014 None:intergenic 35.0%
TTAGTGCAGGGAAGAAATAA+TGG - chr8_1:72116996-72117015 None:intergenic 35.0%
!! CATAGGACAATTTTAGTGCA+GGG - chr8_1:72117008-72117027 None:intergenic 35.0%
AATGCTGATCCAATTCCATT+AGG + chr8_1:72117106-72117125 Msa1212270:three_prime_UTR 35.0%
AGTTCAGACAAACTCTTGTT+TGG + chr8_1:72117152-72117171 Msa1212270:three_prime_UTR 35.0%
! TAATTGGAACTATTTGGAGC+AGG + chr8_1:72117283-72117302 Msa1212270:three_prime_UTR 35.0%
AAAATGGGGTTTGCGAGATT+GGG - chr8_1:72112843-72112862 None:intergenic 40.0%
!! GCATAGGACAATTTTAGTGC+AGG - chr8_1:72117009-72117028 None:intergenic 40.0%
GCACTAAAATTGTCCTATGC+AGG + chr8_1:72117009-72117028 Msa1212270:three_prime_UTR 40.0%
GAAAATGGGGTTTGCGAGAT+TGG - chr8_1:72112844-72112863 None:intergenic 45.0%
TTCTCAAGCATCCTTAGGAG+AGG - chr8_1:72116953-72116972 None:intergenic 45.0%
TCTCCTAAGGATGCTTGAGA+AGG + chr8_1:72116952-72116971 Msa1212270:exon 45.0%
TGACCTTCTCAAGCATCCTT+AGG - chr8_1:72116958-72116977 None:intergenic 45.0%
TCCATCAAAGCAAGGATCAG+AGG - chr8_1:72117040-72117059 None:intergenic 45.0%
AGAGTTTGTCTGAACTCCAC+AGG - chr8_1:72117148-72117167 None:intergenic 45.0%
ACTCATTGGACATAACCAGC+AGG - chr8_1:72117241-72117260 None:intergenic 45.0%
ATCCTTAGGAGAGGAATCCC+TGG - chr8_1:72116944-72116963 None:intergenic 50.0%
ATCAGAGGCAAGTCCTGCAT+AGG - chr8_1:72117025-72117044 None:intergenic 50.0%
GCCTCTGATCCTTGCTTTGA+TGG + chr8_1:72117036-72117055 Msa1212270:three_prime_UTR 50.0%
!! CTGAGGGTGCCTAATGGAAT+TGG - chr8_1:72117118-72117137 None:intergenic 50.0%
CAATTCCATTAGGCACCCTC+AGG + chr8_1:72117116-72117135 Msa1212270:three_prime_UTR 50.0%
TGTCTGAACTCCACAGGATG+AGG - chr8_1:72117142-72117161 None:intergenic 50.0%
TCCTACTGAGCTCTTGCAGA+AGG + chr8_1:72117208-72117227 Msa1212270:three_prime_UTR 50.0%
ACCTTCTGCAAGAGCTCAGT+AGG - chr8_1:72117212-72117231 None:intergenic 50.0%
CATTGGACATAACCAGCAGG+AGG - chr8_1:72117238-72117257 None:intergenic 50.0%
! GAACTATTTGGAGCAGGCTG+TGG + chr8_1:72117289-72117308 Msa1212270:three_prime_UTR 50.0%
!! TGAGGCCTGAGGGTGCCTAA+TGG - chr8_1:72117124-72117143 None:intergenic 60.0%
CTCCACAGGATGAGGCCTGA+GGG - chr8_1:72117134-72117153 None:intergenic 60.0%
ACTCCACAGGATGAGGCCTG+AGG - chr8_1:72117135-72117154 None:intergenic 60.0%
GCAGAAGGTGAACCTCCTGC+TGG + chr8_1:72117223-72117242 Msa1212270:three_prime_UTR 60.0%
CACCCTCAGGCCTCATCCTG+TGG + chr8_1:72117129-72117148 Msa1212270:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 72112832 72117409 72112832 ID=Msa1212270;Name=Msa1212270
chr8_1 mRNA 72112832 72117409 72112832 ID=Msa1212270-mRNA-1;Parent=Msa1212270;Name=Msa1212270-mRNA-1;_AED=0.05;_eAED=0.05;_QI=251|1|1|1|1|1|7|440|585
chr8_1 exon 72112832 72113257 72112832 ID=Msa1212270-mRNA-1:exon:24294;Parent=Msa1212270-mRNA-1
chr8_1 exon 72114222 72114383 72114222 ID=Msa1212270-mRNA-1:exon:24295;Parent=Msa1212270-mRNA-1
chr8_1 exon 72114490 72114623 72114490 ID=Msa1212270-mRNA-1:exon:24296;Parent=Msa1212270-mRNA-1
chr8_1 exon 72115123 72115314 72115123 ID=Msa1212270-mRNA-1:exon:24297;Parent=Msa1212270-mRNA-1
chr8_1 exon 72115462 72115564 72115462 ID=Msa1212270-mRNA-1:exon:24298;Parent=Msa1212270-mRNA-1
chr8_1 exon 72115797 72115850 72115797 ID=Msa1212270-mRNA-1:exon:24299;Parent=Msa1212270-mRNA-1
chr8_1 exon 72116032 72117409 72116032 ID=Msa1212270-mRNA-1:exon:24300;Parent=Msa1212270-mRNA-1
chr8_1 five_prime_UTR 72112832 72113082 72112832 ID=Msa1212270-mRNA-1:five_prime_utr;Parent=Msa1212270-mRNA-1
chr8_1 CDS 72113083 72113257 72113083 ID=Msa1212270-mRNA-1:cds;Parent=Msa1212270-mRNA-1
chr8_1 CDS 72114222 72114383 72114222 ID=Msa1212270-mRNA-1:cds;Parent=Msa1212270-mRNA-1
chr8_1 CDS 72114490 72114623 72114490 ID=Msa1212270-mRNA-1:cds;Parent=Msa1212270-mRNA-1
chr8_1 CDS 72115123 72115314 72115123 ID=Msa1212270-mRNA-1:cds;Parent=Msa1212270-mRNA-1
chr8_1 CDS 72115462 72115564 72115462 ID=Msa1212270-mRNA-1:cds;Parent=Msa1212270-mRNA-1
chr8_1 CDS 72115797 72115850 72115797 ID=Msa1212270-mRNA-1:cds;Parent=Msa1212270-mRNA-1
chr8_1 CDS 72116032 72116969 72116032 ID=Msa1212270-mRNA-1:cds;Parent=Msa1212270-mRNA-1
chr8_1 three_prime_UTR 72116970 72117409 72116970 ID=Msa1212270-mRNA-1:three_prime_utr;Parent=Msa1212270-mRNA-1
Gene Sequence

>Msa1212270

ATGGATACTGAAGAACTTATTGATTTGAACAGTAAAGAGGTTAATTGTGGTGGAAATAATAGCAAGAGTGGTGATGGGTTCATTGATAGGAGTAAAGTTAGGATTTTGCTGTGTGATAGTGATTCCAAGAGTTCAGAAGAGGTTTTTACTCTTCTTATTAATTGCTCTTATCAGGTTACGTCTGTAAAATCTGCACGGCAAGTGATTGACGCATTGAATGCGGAAGGACAATATATAGATATTATACTAGCGGAAGTTGACCTTCCAATAAAAAAGGGAATGAAGATGTTGAAGTACATAGCACGGGATAAAGAACTGCGCCAAATTCCTATTATAATGATGTCTGCACAAGATGAGGTATCAATTGTTGTCAAGTGCTTGAGGCTCGGAGCAGCAGACTATCTAGTAAAGCCTTTACGTACTAATGAACTATTAAATTTGTGGACGCACATGTGGAGAAGGAGACGCATGCTTGGACTTATAGAGAACAACATCTTGAATTATGATTTTGATCTGGTAGCATCAGACCCTAGTGATGCCAATACAAACAGTGCCACCTTCTTCTCTGATGACACAGACGACAAGTCCAAAAGAAGCAATAATCCCGAGGCTGGAATTTCAATCCAACAAGAACAGGCGGAAATTTCAATCCAACAAGAAGAGGCTAGTATTGCCACTGCTGCTGCTGTTATTGAGGAGCATCCATATGTTCATGTATCAGAATATCAGCCTGATGTACCTGGAATTAGTGATCGAAGAAGAACAGGTTCTTCAGCACAGCATCCGCCGTGCAACAGAGTTACAAATAGTAGCTTTGATGGTCCGAAGAAGAGTGAACTCAGAATTGGAGAATCATCAGCCTTTTTCACTTATGTCAAAGCAACAACAATGAAGAGCAACTTGGAAGAGATTGCTCATTCTGACAATAGCGGTACATTACTAGTGAGGGTGGAAGATATGAATCAAGCATGTTCTCAACAAGGGGGTAATGACCTTATAAGACATGAAAATGGAGAAACGGTTGAAAGCCATTCACAAGATGACTTACCTAGAAGCAATAGTATCCCCGATTCTTTCTCTGTTGAGAGATCGTGTACCCCACCTGCATCAATGGAGGTTTCACAGCAAAATAGTAATCACAGGGAAGAACATCCACAAGGGGTGGTTCATCCAAGAAATGGAAGTTATGGTTCTGAGCTCAACCAATCGGGCATGCCTGCCCAACATGCTTATCCGTATTATATGCCAGGAGTTGCTAATCATGTTATGATGCAGTCATCAACACATGTGTATCAAAAGAATATCCAGAACCTTCAGAGTCATGTTAGTTCATCTATGATTTCCCAGTACAATCAACTTCCCCAATGTCCACCTCATGCAACTGGGATGACACCCTACCCATATTTTCCCATGAGTATTTGTTTACAGACCGGTCAGGTTTCTACAGCTCATCCATGGCCGTCATTAGGAAGTTCAACTTCATGTGAAGCAAACTTCAGAAAAGTCGATAGGAGAGAAGCAGCATTGATGAAGTTTAGACAGAAAAGAAAAGAGCGTTGCTTCGATAAGAAAATTAGGTACGTCAATCGGAAACAACTTGCAGAAAGGCGGCCTCGTGTTAGGGGACAGTTTGTCAGGAAGTTGAATGGTATTAATGTGGATCTAAATGGACAACCTGCTTCCACCGACTATGACGATGAGGATGAAGAGGAGGAAGAAAACAATGACACCAGGGATTCCTCTCCTAAGGATGCTTGA
Protein sequence

>Msa1212270

MDTEELIDLNSKEVNCGGNNSKSGDGFIDRSKVRILLCDSDSKSSEEVFTLLINCSYQVTSVKSARQVIDALNAEGQYIDIILAEVDLPIKKGMKMLKYIARDKELRQIPIIMMSAQDEVSIVVKCLRLGAADYLVKPLRTNELLNLWTHMWRRRRMLGLIENNILNYDFDLVASDPSDANTNSATFFSDDTDDKSKRSNNPEAGISIQQEQAEISIQQEEASIATAAAVIEEHPYVHVSEYQPDVPGISDRRRTGSSAQHPPCNRVTNSSFDGPKKSELRIGESSAFFTYVKATTMKSNLEEIAHSDNSGTLLVRVEDMNQACSQQGGNDLIRHENGETVESHSQDDLPRSNSIPDSFSVERSCTPPASMEVSQQNSNHREEHPQGVVHPRNGSYGSELNQSGMPAQHAYPYYMPGVANHVMMQSSTHVYQKNIQNLQSHVSSSMISQYNQLPQCPPHATGMTPYPYFPMSICLQTGQVSTAHPWPSLGSSTSCEANFRKVDRREAALMKFRQKRKERCFDKKIRYVNRKQLAERRPRVRGQFVRKLNGINVDLNGQPASTDYDDEDEEEEENNDTRDSSPKDA*