AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605630 MtrunA17_Chr4g0035001 98.795 83 1 0 1 83 1 83 1.34e-57 177
Msa0605630 MtrunA17_Chr4g0035051 90.244 82 8 0 2 83 4 85 1.90e-52 162
Msa0605630 MtrunA17_Chr4g0035031 84.337 83 13 0 2 84 4 86 1.06e-48 155
Msa0605630 MtrunA17_Chr2g0278661 57.955 88 36 1 1 87 1 88 1.75e-33 118
Msa0605630 MtrunA17_Chr4g0050781 55.814 86 37 1 1 85 1 86 9.86e-31 110
Msa0605630 MtrunA17_Chr3g0116631 54.321 81 37 0 6 86 206 286 4.44e-27 102
Msa0605630 MtrunA17_Chr3g0116631 33.000 100 67 0 7 106 110 209 2.85e-12 63.2
Msa0605630 MtrunA17_Chr4g0061451 52.500 80 38 0 7 86 43 122 2.53e-25 97.8
Msa0605630 MtrunA17_Chr4g0058151 53.086 81 38 0 6 86 216 296 1.01e-24 97.1
Msa0605630 MtrunA17_Chr4g0058151 35.577 104 64 1 5 108 121 221 1.01e-14 70.1
Msa0605630 MtrunA17_Chr1g0210241 49.383 81 41 0 5 85 6 86 4.24e-23 92.0
Msa0605630 MtrunA17_Chr3g0086861 50.633 79 39 0 7 85 45 123 5.03e-23 94.0
Msa0605630 MtrunA17_Chr1g0181111 51.250 80 39 0 7 86 197 276 3.84e-21 87.0
Msa0605630 MtrunA17_Chr7g0219021 53.782 119 55 0 7 125 41 159 1.45e-20 85.1
Msa0605630 MtrunA17_Chr3g0119311 42.683 82 47 0 7 88 37 118 2.23e-19 79.0
Msa0605630 MtrunA17_Chr7g0266751 39.583 96 56 1 4 97 30 125 8.10e-19 77.8
Msa0605630 MtrunA17_Chr1g0168601 40.244 82 49 0 2 83 149 230 8.42e-18 77.4
Msa0605630 MtrunA17_Chr3g0121631 48.760 121 51 2 7 127 37 146 1.88e-17 74.3
Msa0605630 MtrunA17_Chr4g0017401 40.000 115 58 5 3 108 12 124 1.09e-16 74.7
Msa0605630 MtrunA17_Chr3g0127751 32.117 137 79 3 7 132 27 160 1.37e-16 74.3
Msa0605630 MtrunA17_Chr7g0219031 45.455 66 36 0 7 72 41 106 2.19e-16 70.9
Msa0605630 MtrunA17_Chr3g0101061 37.975 79 47 1 7 85 41 117 1.40e-15 73.2
Msa0605630 MtrunA17_Chr7g0276531 50.847 59 29 0 7 65 19 77 6.31e-15 70.1
Msa0605630 MtrunA17_Chr2g0290191 37.975 79 47 1 7 85 5 81 7.03e-15 70.9
Msa0605630 MtrunA17_Chr3g0144311 40.741 81 46 2 3 82 25 104 7.55e-15 70.1
Msa0605630 MtrunA17_Chr1g0186351 32.911 79 51 1 7 85 7 83 1.32e-14 70.1
Msa0605630 MtrunA17_Chr1g0186351 32.955 88 58 1 7 94 113 199 7.21e-13 65.1
Msa0605630 MtrunA17_Chr7g0263001 39.241 79 48 0 7 85 36 114 1.52e-14 66.6
Msa0605630 MtrunA17_Chr5g0447761 36.709 79 48 1 7 85 7 83 1.66e-14 70.1
Msa0605630 MtrunA17_Chr5g0447761 32.258 93 62 1 7 99 107 198 5.78e-12 62.8
Msa0605630 MtrunA17_Chr6g0481071 37.500 88 55 0 7 94 96 183 2.10e-14 67.4
Msa0605630 MtrunA17_Chr4g0018581 46.667 75 40 0 8 82 453 527 2.33e-14 69.7
Msa0605630 MtrunA17_Chr5g0426311 43.421 76 42 1 7 82 17 91 2.53e-14 68.6
Msa0605630 MtrunA17_Chr3g0120721 34.328 134 83 3 7 139 107 236 2.75e-14 68.9
Msa0605630 MtrunA17_Chr3g0120721 31.068 103 69 1 7 109 19 119 1.92e-13 66.6
Msa0605630 MtrunA17_Chr6g0449481 35.417 96 60 1 7 102 46 139 2.93e-14 68.9
Msa0605630 MtrunA17_Chr6g0449481 33.333 132 74 2 7 124 134 265 3.33e-11 60.5
Msa0605630 MtrunA17_Chr2g0327921 36.585 82 52 0 7 88 8 89 3.57e-14 64.3
Msa0605630 MtrunA17_Chr4g0042161 36.275 102 59 2 1 99 46 144 6.85e-14 65.1
Msa0605630 MtrunA17_Chr8g0346011 42.308 78 40 2 7 82 38 112 8.07e-14 67.4
Msa0605630 MtrunA17_Chr3g0110211 35.514 107 68 1 8 113 114 220 3.74e-13 65.9
Msa0605630 MtrunA17_Chr4g0062931 35.256 156 91 5 8 158 9 159 4.19e-13 65.9
Msa0605630 MtrunA17_Chr3g0088511 43.243 74 42 0 7 80 33 106 6.47e-13 62.4
Msa0605630 MtrunA17_Chr4g0031091 38.356 73 45 0 7 79 34 106 9.62e-13 61.2
Msa0605630 MtrunA17_Chr5g0402191 40.000 80 44 2 7 85 5 81 1.17e-12 64.7
Msa0605630 MtrunA17_Chr3g0135111 35.714 98 58 2 7 101 11 106 2.17e-12 63.5
Msa0605630 MtrunA17_Chr3g0135111 27.778 126 89 2 7 132 118 241 8.66e-11 59.3
Msa0605630 MtrunA17_Chr4g0065741 37.662 77 48 0 7 83 72 148 3.77e-12 60.8
Msa0605630 MtrunA17_Chr7g0267571 36.667 90 57 0 2 91 208 297 6.96e-12 62.0
Msa0605630 MtrunA17_Chr4g0050441 38.462 78 48 0 6 83 16 93 1.26e-11 61.2
Msa0605630 MtrunA17_Chr5g0429941 38.554 83 50 1 1 83 12 93 1.27e-11 60.8
Msa0605630 MtrunA17_Chr8g0360551 37.209 86 48 3 7 90 74 155 5.39e-11 59.7
Msa0605630 MtrunA17_Chr5g0419351 34.343 99 65 0 8 106 12 110 8.50e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605630 AT4G39260.3 74.725 91 23 0 1 91 1 91 3.02e-48 151
Msa0605630 AT2G21660.2 82.716 81 14 0 3 83 5 85 3.97e-46 148
Msa0605630 AT2G21660.1 82.716 81 14 0 3 83 5 85 6.64e-46 148
Msa0605630 AT4G39260.2 72.289 83 23 0 1 83 1 83 5.99e-43 139
Msa0605630 AT4G39260.1 72.289 83 23 0 1 83 1 83 2.19e-42 139
Msa0605630 AT4G39260.4 72.059 68 19 0 1 68 1 68 3.08e-34 116
Msa0605630 AT3G26420.1 35.321 218 80 4 3 159 4 221 8.15e-29 106
Msa0605630 AT5G61030.2 53.165 79 37 0 7 85 41 119 4.19e-26 101
Msa0605630 AT5G61030.1 53.165 79 37 0 7 85 41 119 4.19e-26 101
Msa0605630 AT3G23830.2 53.191 94 44 0 7 100 36 129 5.37e-26 96.7
Msa0605630 AT3G23830.1 53.191 94 44 0 7 100 36 129 5.37e-26 96.7
Msa0605630 AT5G50250.1 51.807 83 40 0 3 85 204 286 6.30e-25 97.4
Msa0605630 AT5G50250.1 40.000 90 54 0 5 94 112 201 1.01e-14 70.1
Msa0605630 AT4G13850.4 53.086 81 38 0 7 87 36 116 1.14e-24 92.8
Msa0605630 AT4G24770.1 50.000 82 41 0 6 87 244 325 1.31e-24 97.4
Msa0605630 AT4G24770.1 42.222 90 52 0 5 94 149 238 1.21e-15 73.2
Msa0605630 AT4G24770.2 50.000 82 41 0 6 87 244 325 1.31e-24 97.4
Msa0605630 AT4G24770.2 42.222 90 52 0 5 94 149 238 1.21e-15 73.2
Msa0605630 AT4G13850.1 52.525 99 47 0 7 105 36 134 3.75e-23 89.7
Msa0605630 AT5G04280.1 47.312 93 46 1 7 96 8 100 4.68e-23 93.2
Msa0605630 AT2G21690.1 49.438 89 40 1 2 85 3 91 9.31e-22 85.1
Msa0605630 AT2G37220.1 45.679 81 44 0 7 87 205 285 3.90e-21 87.4
Msa0605630 AT1G74230.1 57.647 85 35 1 7 91 35 118 4.56e-21 87.4
Msa0605630 AT3G53460.1 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0605630 AT3G53460.3 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0605630 AT3G53460.2 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0605630 AT3G53460.4 45.783 83 45 0 7 89 279 361 7.04e-21 87.8
Msa0605630 AT5G06210.1 45.556 90 49 0 4 93 32 121 1.07e-20 83.2
Msa0605630 AT1G60650.2 41.121 107 62 1 4 109 10 116 1.12e-19 83.6
Msa0605630 AT1G60650.1 41.121 107 62 1 4 109 10 116 1.12e-19 83.6
Msa0605630 AT4G13850.2 46.552 116 49 1 7 122 36 138 3.21e-19 79.7
Msa0605630 AT4G13850.3 44.628 121 45 1 7 127 36 134 2.15e-18 77.0
Msa0605630 AT5G47320.1 46.753 77 41 0 7 83 32 108 2.56e-17 75.9
Msa0605630 AT1G18630.1 52.083 96 46 0 7 102 37 132 2.77e-17 74.7
Msa0605630 AT1G22330.1 43.137 102 52 3 7 103 18 118 4.79e-17 76.6
Msa0605630 AT2G46780.1 46.053 76 40 1 7 82 23 97 7.97e-17 76.3
Msa0605630 AT2G46780.2 46.053 76 40 1 7 82 23 97 7.97e-17 76.3
Msa0605630 AT4G13860.1 44.595 74 41 0 7 80 4 77 2.19e-16 70.5
Msa0605630 AT4G14300.1 37.975 79 47 1 7 85 7 83 3.55e-16 75.1
Msa0605630 AT4G14300.1 34.177 79 51 1 7 85 111 188 7.97e-11 59.7
Msa0605630 AT4G14300.2 37.975 79 47 1 7 85 7 83 3.55e-16 75.1
Msa0605630 AT4G14300.2 34.177 79 51 1 7 85 111 188 7.97e-11 59.7
Msa0605630 AT3G13224.3 38.710 93 51 2 1 89 1 91 4.35e-16 74.3
Msa0605630 AT3G13224.3 32.323 99 63 1 7 105 101 195 1.87e-14 70.1
Msa0605630 AT3G07810.2 33.028 109 71 1 7 115 7 113 8.47e-16 73.9
Msa0605630 AT3G07810.1 33.028 109 71 1 7 115 7 113 9.69e-16 73.9
Msa0605630 AT2G37510.1 45.946 74 40 0 7 80 35 108 1.04e-15 70.1
Msa0605630 AT5G59865.1 44.304 79 44 0 9 87 29 107 1.33e-15 68.9
Msa0605630 AT3G13224.2 39.759 83 48 1 7 89 20 100 1.44e-15 73.2
Msa0605630 AT3G13224.2 34.568 81 53 0 7 87 110 190 1.12e-14 70.5
Msa0605630 AT1G78260.1 44.444 81 43 2 3 82 13 92 1.65e-15 72.4
Msa0605630 AT1G76460.1 44.737 76 41 1 7 82 25 99 2.12e-15 72.0
Msa0605630 AT3G08000.1 44.048 84 47 0 7 90 42 125 2.64e-15 69.3
Msa0605630 AT4G26650.1 39.241 79 46 1 7 85 16 92 2.78e-15 72.4
Msa0605630 AT4G26650.2 39.241 79 46 1 7 85 13 89 2.97e-15 72.4
Msa0605630 AT1G78260.2 44.444 81 43 2 3 82 13 92 3.03e-15 71.2
Msa0605630 AT1G22910.1 36.364 99 56 3 3 94 9 107 4.12e-15 70.9
Msa0605630 AT1G20880.2 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0605630 AT1G20880.3 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0605630 AT1G20880.1 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0605630 AT2G33410.1 32.558 86 56 1 7 92 7 90 5.83e-15 71.6
Msa0605630 AT2G33410.1 37.975 79 48 1 7 85 111 188 3.68e-12 63.5
Msa0605630 AT1G22910.2 36.364 99 56 3 3 94 9 107 6.11e-15 70.1
Msa0605630 AT5G55550.5 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0605630 AT5G55550.4 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0605630 AT5G55550.9 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0605630 AT5G55550.7 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0605630 AT5G55550.1 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0605630 AT1G60000.1 40.244 82 49 0 2 83 173 254 7.05e-15 70.1
Msa0605630 AT5G55550.2 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0605630 AT5G55550.3 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0605630 AT5G55550.6 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0605630 AT5G55550.11 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0605630 AT1G33470.3 37.500 104 58 3 1 98 1 103 1.53e-14 68.9
Msa0605630 AT1G33470.1 37.500 104 58 3 1 98 1 103 1.79e-14 68.9
Msa0605630 AT1G33470.2 37.500 104 58 3 1 98 1 103 1.80e-14 68.9
Msa0605630 AT5G19960.1 43.038 79 44 1 8 86 9 86 2.42e-14 69.7
Msa0605630 AT1G22910.3 36.364 99 56 3 3 94 9 107 4.40e-14 68.9
Msa0605630 AT3G13224.1 39.286 84 49 1 7 90 20 101 1.09e-13 66.6
Msa0605630 AT5G55550.8 42.254 71 39 1 7 77 7 75 1.88e-13 67.4
Msa0605630 AT5G51300.1 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0605630 AT5G51300.2 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0605630 AT5G51300.3 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0605630 AT5G55550.10 42.254 71 39 1 7 77 7 75 2.38e-13 67.0
Msa0605630 AT3G54770.3 42.105 76 43 1 7 82 18 92 2.76e-13 65.9
Msa0605630 AT3G46020.1 37.662 77 48 0 7 83 8 84 2.99e-13 62.8
Msa0605630 AT3G54770.1 42.105 76 43 1 7 82 18 92 2.99e-13 65.9
Msa0605630 AT5G47620.1 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0605630 AT5G47620.2 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0605630 AT5G47620.5 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0605630 AT5G40490.1 36.458 96 59 1 7 102 43 136 3.80e-13 66.2
Msa0605630 AT5G40490.1 32.292 96 63 1 7 100 131 226 1.14e-11 62.0
Msa0605630 AT1G17640.2 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0605630 AT1G17640.2 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0605630 AT1G17640.1 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0605630 AT1G17640.1 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0605630 AT1G17640.3 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0605630 AT1G17640.3 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0605630 AT3G52150.2 40.741 81 48 0 2 82 173 253 6.28e-13 64.7
Msa0605630 AT3G52150.1 40.741 81 48 0 2 82 173 253 6.28e-13 64.7
Msa0605630 AT2G27330.1 31.579 76 52 0 9 84 24 99 6.86e-13 62.0
Msa0605630 AT1G73530.1 31.818 88 60 0 7 94 78 165 9.92e-13 63.2
Msa0605630 AT1G01080.1 37.349 83 47 2 1 81 207 286 1.61e-12 63.9
Msa0605630 AT5G53720.1 41.250 80 46 1 3 82 6 84 1.63e-12 60.8
Msa0605630 AT1G01080.2 37.349 83 47 2 1 81 208 287 1.74e-12 63.9
Msa0605630 AT5G64200.2 39.744 78 47 0 6 83 16 93 3.81e-12 63.2
Msa0605630 AT5G64200.1 39.744 78 47 0 6 83 16 93 3.81e-12 63.2
Msa0605630 AT3G14100.1 39.744 78 47 0 5 82 143 220 4.53e-12 63.2
Msa0605630 AT5G47620.4 36.709 79 44 2 7 81 7 83 7.13e-12 62.8
Msa0605630 AT4G13860.2 45.763 59 32 0 22 80 2 60 1.12e-11 57.8
Msa0605630 AT1G54080.1 39.744 78 47 0 5 82 147 224 2.05e-11 61.2
Msa0605630 AT5G11412.1 42.169 83 44 3 2 82 47 127 2.56e-11 59.3
Msa0605630 AT4G36960.1 39.759 83 46 2 4 85 1 80 3.52e-11 60.8
Msa0605630 AT4G36960.2 39.759 83 46 2 4 85 1 80 3.52e-11 60.8
Msa0605630 AT5G04280.3 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0605630 AT5G04280.4 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0605630 AT5G04280.5 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0605630 AT5G04280.2 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0605630 AT5G40490.2 35.052 97 59 3 7 100 114 209 4.46e-11 60.5
Msa0605630 AT2G18510.1 30.081 123 85 1 8 129 114 236 5.95e-11 60.1
Msa0605630 AT5G54580.1 41.026 78 46 0 8 85 58 135 6.31e-11 57.8
Msa0605630 AT1G01080.3 40.000 55 33 0 1 55 207 261 9.93e-11 58.9

Find 88 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAGGTGATGGTGGTTATT+AGG 0.231697 4_2:+51573280 Msa0605630:CDS
GGAGGTGGTGGTGGTGGATA+TGG 0.236629 4_2:+51573107 Msa0605630:CDS
GGTGGAAGCGGTGGTGGATA+TGG 0.268521 4_2:+51573149 Msa0605630:CDS
GGTGGAGGAGGTGGTGGATA+TGG 0.274998 4_2:+51573170 Msa0605630:CDS
ACTGGACGATCAAGAGGTTT+TGG 0.283486 4_2:+51572936 Msa0605630:CDS
ATCATTAACGATCGTGAAAC+TGG 0.286078 4_2:+51572918 Msa0605630:CDS
GGCCGTGGTGGCGGTGGATA+TGG 0.292757 4_2:+51573086 Msa0605630:CDS
AGCAATGAACGGTCAGGATT+TGG 0.313573 4_2:+51573004 Msa0605630:CDS
TGCTTCAATGGCATCGTTCA+TGG 0.319166 4_2:-51572985 None:intergenic
GGAGGCGGTGGTGGAGGATA+TGG 0.330951 4_2:+51573236 Msa0605630:CDS
CACTTAAAACACAGAGAATA+TGG 0.341695 4_2:-51573478 None:intergenic
GGAGGAGGTGGTGGATATGG+TGG 0.343759 4_2:+51573173 Msa0605630:CDS
GGTGGATATGGAGGTGGTGG+TGG 0.355416 4_2:+51573098 Msa0605630:CDS
GGTGGATATGGTGGAGGAGG+TGG 0.362609 4_2:+51573161 Msa0605630:CDS
ATGATTGTTATTCTAGATGT+TGG 0.374897 4_2:+51573322 Msa0605630:three_prime_UTR
GGTGGAGAGCGTCGTGGATA+TGG 0.378333 4_2:+51573128 Msa0605630:CDS
CTGACCGTTCATTGCTTCAA+TGG 0.378604 4_2:-51572997 None:intergenic
GGCGGAGGAGGCCGTGGTGG+CGG 0.382681 4_2:+51573077 Msa0605630:CDS
GGATATGGTGGAGGAGGTGG+TGG 0.390425 4_2:+51573164 Msa0605630:CDS
GGCGGTGGTGGAGGATATGG+TGG 0.396144 4_2:+51573239 Msa0605630:CDS
TACAGCAGAGGAGGCGGTGG+TGG 0.397231 4_2:+51573227 Msa0605630:CDS
GGATATGGAGGTGGTGGTGG+TGG 0.398226 4_2:+51573101 Msa0605630:CDS
CGCTACCACGGCTCTGAGCT+TGG 0.401331 4_2:-51573049 None:intergenic
TGATTGTTATTCTAGATGTT+GGG 0.408580 4_2:+51573323 Msa0605630:three_prime_UTR
GGCGGTGGATATGGAGGTGG+TGG 0.414719 4_2:+51573095 Msa0605630:CDS
GGTGGTGACCGCCGTGGTGG+AGG 0.417792 4_2:+51573197 Msa0605630:CDS
GGTGGTGGTGGTGGATATGG+TGG 0.419545 4_2:+51573110 Msa0605630:CDS
GGAGGTGGTGGATATGGTGG+TGG 0.423953 4_2:+51573176 Msa0605630:CDS
GGTAGCGGTGGTGGCGGAGG+AGG 0.428094 4_2:+51573065 Msa0605630:CDS
GACCGCCGTGGTGGAGGTGG+TGG 0.428431 4_2:+51573203 Msa0605630:CDS
CGTGGTAGCGGTGGTGGCGG+AGG 0.433194 4_2:+51573062 Msa0605630:CDS
CAGAGCCGTGGTAGCGGTGG+TGG 0.439087 4_2:+51573056 Msa0605630:CDS
GGTGGTGGCGGAGGAGGCCG+TGG 0.439963 4_2:+51573071 Msa0605630:CDS
GGTGACCGCCGTGGTGGAGG+TGG 0.440014 4_2:+51573200 Msa0605630:CDS
AGCAGAGGAGGCGGTGGTGG+AGG 0.451847 4_2:+51573230 Msa0605630:CDS
GGATATGGTGGAAGCGGTGG+TGG 0.454383 4_2:+51573143 Msa0605630:CDS
GGAGGAGGCCGTGGTGGCGG+TGG 0.459076 4_2:+51573080 Msa0605630:CDS
GAGAGAGATTATAGAGGCAA+AGG 0.460980 4_2:-51572417 None:intergenic
AGCCGTGGTAGCGGTGGTGG+CGG 0.473368 4_2:+51573059 Msa0605630:CDS
GGAAGCGGTGGTGGATATGG+TGG 0.474255 4_2:+51573152 Msa0605630:CDS
GCTTCGTCGGAGGTCTTGCA+TGG 0.475042 4_2:+51572501 Msa0605630:CDS
GGTGGTGGAGGATATGGTGG+TGG 0.479646 4_2:+51573242 Msa0605630:CDS
TTGGGAGAGAGAGATTATAG+AGG 0.481400 4_2:-51572423 None:intergenic
GGTGGCGGAGGAGGCCGTGG+TGG 0.482575 4_2:+51573074 Msa0605630:CDS
GGTGGTGGATATGGTGGTGG+TGG 0.485578 4_2:+51573179 Msa0605630:CDS
GGTGGAGGATATGGTGGTGG+TGG 0.501369 4_2:+51573245 Msa0605630:CDS
GGATACAGCAGAGGAGGCGG+TGG 0.503411 4_2:+51573224 Msa0605630:CDS
CAGCCATTGAAAAGAGCAAA+GGG 0.505087 4_2:-51572463 None:intergenic
GGTGGATACAGCAGAGGAGG+CGG 0.513743 4_2:+51573221 Msa0605630:CDS
ACAAAACACAGGTAATACTT+TGG 0.514149 4_2:-51573551 None:intergenic
CATTGAAGCAATGAACGGTC+AGG 0.516837 4_2:+51572998 Msa0605630:CDS
CAAGACCTCCGACGAAGCAT+CGG 0.524569 4_2:-51572496 None:intergenic
GTTGAATACCGATGCTTCGT+CGG 0.525860 4_2:+51572488 Msa0605630:CDS
TACAGCAGAGGAGGAGGTGA+TGG 0.532656 4_2:+51573269 Msa0605630:CDS
CAAGCTCAGAGCCGTGGTAG+CGG 0.533686 4_2:+51573050 Msa0605630:CDS
CGGCGAGATCGTTGATTCCA+AGG 0.539852 4_2:+51572565 Msa0605630:CDS
AGCGGTGGTGGATATGGTGG+AGG 0.541831 4_2:+51573155 Msa0605630:CDS
GAGAAAGCCTTCTCTCAATA+CGG 0.543855 4_2:+51572545 Msa0605630:CDS
TTTCTCGAGAGCATCGTTGT+CGG 0.548634 4_2:-51572528 None:intergenic
CGTGAAACTGGACGATCAAG+AGG 0.551662 4_2:+51572930 Msa0605630:CDS
CGTGGTGGCGGTGGATATGG+AGG 0.555369 4_2:+51573089 Msa0605630:CDS
GGTGGATACAGCAGAGGAGG+AGG 0.557224 4_2:+51573263 Msa0605630:CDS
GGTGGTGGATATGGTGGAGG+AGG 0.560670 4_2:+51573158 Msa0605630:CDS
CGTCGTGGATATGGTGGAAG+CGG 0.562578 4_2:+51573137 Msa0605630:CDS
GGTGGTGGATACAGCAGAGG+AGG 0.562608 4_2:+51573218 Msa0605630:CDS
CGTGGATATGGTGGAAGCGG+TGG 0.562637 4_2:+51573140 Msa0605630:CDS
GGTGGTGGTGACCGCCGTGG+TGG 0.562802 4_2:+51573194 Msa0605630:CDS
CTCCATATCCACCGCCACCA+CGG 0.564840 4_2:-51573088 None:intergenic
GCTCAGAGCCGTGGTAGCGG+TGG 0.565666 4_2:+51573053 Msa0605630:CDS
GGTGGCGGTGGATATGGAGG+TGG 0.573835 4_2:+51573092 Msa0605630:CDS
GGTGGTGGATACAGCAGAGG+AGG 0.582601 4_2:+51573260 Msa0605630:CDS
CTCGAGAGCATCGTTGTCGG+TGG 0.585909 4_2:-51572525 None:intergenic
GAATACCGATGCTTCGTCGG+AGG 0.590495 4_2:+51572491 Msa0605630:CDS
CTTCGTCGGAGGTCTTGCAT+GGG 0.591814 4_2:+51572502 Msa0605630:CDS
CTCCGCCACCACCGCTACCA+CGG 0.595147 4_2:-51573061 None:intergenic
AGCAGAGGAGGAGGTGATGG+TGG 0.597479 4_2:+51573272 Msa0605630:CDS
TCAGCCATTGAAAAGAGCAA+AGG 0.600296 4_2:-51572464 None:intergenic
ATGAACGGTCAGGATTTGGA+TGG 0.621464 4_2:+51573008 Msa0605630:CDS
GGAGAGCGTCGTGGATATGG+TGG 0.640307 4_2:+51573131 Msa0605630:CDS
GTGAACCAAGCTCAGAGCCG+TGG 0.643330 4_2:+51573044 Msa0605630:CDS
GATCTCGCCGTATTGAGAGA+AGG 0.650761 4_2:-51572552 None:intergenic
GGTGGTGGTGGTGACCGCCG+TGG 0.665791 4_2:+51573191 Msa0605630:CDS
GGAGGTGGTGGATACAGCAG+AGG 0.669900 4_2:+51573215 Msa0605630:CDS
GGTGGTGGTGGATACAGCAG+AGG 0.670896 4_2:+51573257 Msa0605630:CDS
GATGCCATTGAAGCAATGAA+CGG 0.670960 4_2:+51572993 Msa0605630:CDS
TGTATCCACCACCTCCACCA+CGG 0.715403 4_2:-51573208 None:intergenic
GGATATGGTGGAGAGCGTCG+TGG 0.717480 4_2:+51573122 Msa0605630:CDS
ATCCACCACCTCCACCACGG+CGG 0.754503 4_2:-51573205 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTGATGTTT+TGG + chr4_2:51572858-51572877 Msa0605630:intron 10.0%
!!! TTTTTTTTTTTTGATGTTTT+GGG + chr4_2:51572859-51572878 Msa0605630:intron 10.0%
!!! TTAAGTTTATTTTAACTAAT+TGG - chr4_2:51573531-51573550 None:intergenic 10.0%
!!! TTTTTTTTTTTTTGATGTTT+TGG + chr4_2:51572858-51572877 Msa0605630:intron 10.0%
!!! TTTTTTTTTTTTGATGTTTT+GGG + chr4_2:51572859-51572878 Msa0605630:intron 10.0%
!!! TTAAGTTTATTTTAACTAAT+TGG - chr4_2:51573531-51573550 None:intergenic 10.0%
!! ATGATTGTTATTCTAGATGT+TGG + chr4_2:51573322-51573341 Msa0605630:three_prime_UTR 25.0%
!! TGATTGTTATTCTAGATGTT+GGG + chr4_2:51573323-51573342 Msa0605630:three_prime_UTR 25.0%
!!! GTTTATTTTAACTAATTGGC+AGG - chr4_2:51573527-51573546 None:intergenic 25.0%
!! ATGATTGTTATTCTAGATGT+TGG + chr4_2:51573322-51573341 Msa0605630:three_prime_UTR 25.0%
!! TGATTGTTATTCTAGATGTT+GGG + chr4_2:51573323-51573342 Msa0605630:three_prime_UTR 25.0%
!!! GTTTATTTTAACTAATTGGC+AGG - chr4_2:51573527-51573546 None:intergenic 25.0%
GAAAAACTGAATTAGGGTTT+GGG - chr4_2:51572444-51572463 None:intergenic 30.0%
CAAAGGGAAAAACTGAATTA+GGG - chr4_2:51572450-51572469 None:intergenic 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG + chr4_2:51572627-51572646 Msa0605630:intron 30.0%
AACAAAAGTTGAAGATCGAT+CGG + chr4_2:51572803-51572822 Msa0605630:intron 30.0%
!!! TTTTGGGTTTTATTCTGATC+TGG + chr4_2:51572875-51572894 Msa0605630:intron 30.0%
! TTTTTGTCACTGTTCTGTTA+TGG + chr4_2:51573365-51573384 Msa0605630:three_prime_UTR 30.0%
CACTTAAAACACAGAGAATA+TGG - chr4_2:51573481-51573500 None:intergenic 30.0%
GAAAAACTGAATTAGGGTTT+GGG - chr4_2:51572444-51572463 None:intergenic 30.0%
CAAAGGGAAAAACTGAATTA+GGG - chr4_2:51572450-51572469 None:intergenic 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG + chr4_2:51572627-51572646 Msa0605630:intron 30.0%
AACAAAAGTTGAAGATCGAT+CGG + chr4_2:51572803-51572822 Msa0605630:intron 30.0%
!!! TTTTGGGTTTTATTCTGATC+TGG + chr4_2:51572875-51572894 Msa0605630:intron 30.0%
! TTTTTGTCACTGTTCTGTTA+TGG + chr4_2:51573365-51573384 Msa0605630:three_prime_UTR 30.0%
CACTTAAAACACAGAGAATA+TGG - chr4_2:51573481-51573500 None:intergenic 30.0%
GGAAAAACTGAATTAGGGTT+TGG - chr4_2:51572445-51572464 None:intergenic 35.0%
GCAAAGGGAAAAACTGAATT+AGG - chr4_2:51572451-51572470 None:intergenic 35.0%
! TTTCCCTTTGCTCTTTTCAA+TGG + chr4_2:51572460-51572479 Msa0605630:exon 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_2:51572617-51572636 Msa0605630:intron 35.0%
TATTACTCACTCACACCAAT+AGG + chr4_2:51572759-51572778 Msa0605630:intron 35.0%
AAGAAGATGAACGTACCTAT+TGG - chr4_2:51572777-51572796 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG + chr4_2:51572814-51572833 Msa0605630:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG + chr4_2:51572828-51572847 Msa0605630:intron 35.0%
ATCATTAACGATCGTGAAAC+TGG + chr4_2:51572918-51572937 Msa0605630:CDS 35.0%
!! CGTTTTGATGTGTCTCTTAT+TGG + chr4_2:51573391-51573410 Msa0605630:three_prime_UTR 35.0%
GGAAAAACTGAATTAGGGTT+TGG - chr4_2:51572445-51572464 None:intergenic 35.0%
GCAAAGGGAAAAACTGAATT+AGG - chr4_2:51572451-51572470 None:intergenic 35.0%
! TTTCCCTTTGCTCTTTTCAA+TGG + chr4_2:51572460-51572479 Msa0605630:exon 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_2:51572617-51572636 Msa0605630:intron 35.0%
TATTACTCACTCACACCAAT+AGG + chr4_2:51572759-51572778 Msa0605630:intron 35.0%
AAGAAGATGAACGTACCTAT+TGG - chr4_2:51572777-51572796 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG + chr4_2:51572814-51572833 Msa0605630:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG + chr4_2:51572828-51572847 Msa0605630:intron 35.0%
ATCATTAACGATCGTGAAAC+TGG + chr4_2:51572918-51572937 Msa0605630:CDS 35.0%
!! CGTTTTGATGTGTCTCTTAT+TGG + chr4_2:51573391-51573410 Msa0605630:three_prime_UTR 35.0%
GAGAGAGATTATAGAGGCAA+AGG - chr4_2:51572420-51572439 None:intergenic 40.0%
TTGGGAGAGAGAGATTATAG+AGG - chr4_2:51572426-51572445 None:intergenic 40.0%
CAGCCATTGAAAAGAGCAAA+GGG - chr4_2:51572466-51572485 None:intergenic 40.0%
TCAGCCATTGAAAAGAGCAA+AGG - chr4_2:51572467-51572486 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_2:51572545-51572564 Msa0605630:CDS 40.0%
CAGAGTAACACAGAACAGAT+CGG - chr4_2:51572675-51572694 None:intergenic 40.0%
GATGCCATTGAAGCAATGAA+CGG + chr4_2:51572993-51573012 Msa0605630:CDS 40.0%
GAGAGAGATTATAGAGGCAA+AGG - chr4_2:51572420-51572439 None:intergenic 40.0%
TTGGGAGAGAGAGATTATAG+AGG - chr4_2:51572426-51572445 None:intergenic 40.0%
CAGCCATTGAAAAGAGCAAA+GGG - chr4_2:51572466-51572485 None:intergenic 40.0%
TCAGCCATTGAAAAGAGCAA+AGG - chr4_2:51572467-51572486 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_2:51572545-51572564 Msa0605630:CDS 40.0%
CAGAGTAACACAGAACAGAT+CGG - chr4_2:51572675-51572694 None:intergenic 40.0%
GATGCCATTGAAGCAATGAA+CGG + chr4_2:51572993-51573012 Msa0605630:CDS 40.0%
GTTGAATACCGATGCTTCGT+CGG + chr4_2:51572488-51572507 Msa0605630:CDS 45.0%
!! TTTCTCGAGAGCATCGTTGT+CGG - chr4_2:51572531-51572550 None:intergenic 45.0%
TCGGAGATCACAGATCAGAA+AGG - chr4_2:51572656-51572675 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_2:51572936-51572955 Msa0605630:CDS 45.0%
TGCTTCAATGGCATCGTTCA+TGG - chr4_2:51572988-51573007 None:intergenic 45.0%
CTGACCGTTCATTGCTTCAA+TGG - chr4_2:51573000-51573019 None:intergenic 45.0%
CATTGAAGCAATGAACGGTC+AGG + chr4_2:51572998-51573017 Msa0605630:CDS 45.0%
AGCAATGAACGGTCAGGATT+TGG + chr4_2:51573004-51573023 Msa0605630:CDS 45.0%
ATGAACGGTCAGGATTTGGA+TGG + chr4_2:51573008-51573027 Msa0605630:CDS 45.0%
!! AGGAGGTGATGGTGGTTATT+AGG + chr4_2:51573280-51573299 Msa0605630:CDS 45.0%
GTTGAATACCGATGCTTCGT+CGG + chr4_2:51572488-51572507 Msa0605630:CDS 45.0%
!! TTTCTCGAGAGCATCGTTGT+CGG - chr4_2:51572531-51572550 None:intergenic 45.0%
TCGGAGATCACAGATCAGAA+AGG - chr4_2:51572656-51572675 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_2:51572936-51572955 Msa0605630:CDS 45.0%
TGCTTCAATGGCATCGTTCA+TGG - chr4_2:51572988-51573007 None:intergenic 45.0%
CTGACCGTTCATTGCTTCAA+TGG - chr4_2:51573000-51573019 None:intergenic 45.0%
CATTGAAGCAATGAACGGTC+AGG + chr4_2:51572998-51573017 Msa0605630:CDS 45.0%
AGCAATGAACGGTCAGGATT+TGG + chr4_2:51573004-51573023 Msa0605630:CDS 45.0%
ATGAACGGTCAGGATTTGGA+TGG + chr4_2:51573008-51573027 Msa0605630:CDS 45.0%
!! AGGAGGTGATGGTGGTTATT+AGG + chr4_2:51573280-51573299 Msa0605630:CDS 45.0%
GATCTCGCCGTATTGAGAGA+AGG - chr4_2:51572555-51572574 None:intergenic 50.0%
TGCAAGCAACAAACGGACCT+TGG - chr4_2:51572585-51572604 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG + chr4_2:51572584-51572603 Msa0605630:intron 50.0%
CGAAGCCTGCAAGCAACAAA+CGG - chr4_2:51572592-51572611 None:intergenic 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG + chr4_2:51572590-51572609 Msa0605630:intron 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_2:51572930-51572949 Msa0605630:CDS 50.0%
GATCTCGCCGTATTGAGAGA+AGG - chr4_2:51572555-51572574 None:intergenic 50.0%
TGCAAGCAACAAACGGACCT+TGG - chr4_2:51572585-51572604 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG + chr4_2:51572584-51572603 Msa0605630:intron 50.0%
CGAAGCCTGCAAGCAACAAA+CGG - chr4_2:51572592-51572611 None:intergenic 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG + chr4_2:51572590-51572609 Msa0605630:intron 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_2:51572930-51572949 Msa0605630:CDS 50.0%
GAATACCGATGCTTCGTCGG+AGG + chr4_2:51572491-51572510 Msa0605630:CDS 55.0%
CAAGACCTCCGACGAAGCAT+CGG - chr4_2:51572499-51572518 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_2:51572502-51572521 Msa0605630:CDS 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG + chr4_2:51572565-51572584 Msa0605630:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_2:51572595-51572614 Msa0605630:intron 55.0%
! CGTCGTGGATATGGTGGAAG+CGG + chr4_2:51573137-51573156 Msa0605630:CDS 55.0%
TGTATCCACCACCTCCACCA+CGG - chr4_2:51573211-51573230 None:intergenic 55.0%
TACAGCAGAGGAGGAGGTGA+TGG + chr4_2:51573269-51573288 Msa0605630:CDS 55.0%
GAATACCGATGCTTCGTCGG+AGG + chr4_2:51572491-51572510 Msa0605630:CDS 55.0%
CAAGACCTCCGACGAAGCAT+CGG - chr4_2:51572499-51572518 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_2:51572502-51572521 Msa0605630:CDS 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG + chr4_2:51572565-51572584 Msa0605630:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_2:51572595-51572614 Msa0605630:intron 55.0%
! CGTCGTGGATATGGTGGAAG+CGG + chr4_2:51573137-51573156 Msa0605630:CDS 55.0%
TGTATCCACCACCTCCACCA+CGG - chr4_2:51573211-51573230 None:intergenic 55.0%
TACAGCAGAGGAGGAGGTGA+TGG + chr4_2:51573269-51573288 Msa0605630:CDS 55.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_2:51572501-51572520 Msa0605630:CDS 60.0%
!! CTCGAGAGCATCGTTGTCGG+TGG - chr4_2:51572528-51572547 None:intergenic 60.0%
GTGAACCAAGCTCAGAGCCG+TGG + chr4_2:51573044-51573063 Msa0605630:CDS 60.0%
CAAGCTCAGAGCCGTGGTAG+CGG + chr4_2:51573050-51573069 Msa0605630:CDS 60.0%
CTCCATATCCACCGCCACCA+CGG - chr4_2:51573091-51573110 None:intergenic 60.0%
! GGTGGATATGGAGGTGGTGG+TGG + chr4_2:51573098-51573117 Msa0605630:CDS 60.0%
!! GGATATGGAGGTGGTGGTGG+TGG + chr4_2:51573101-51573120 Msa0605630:CDS 60.0%
!! GGAGGTGGTGGTGGTGGATA+TGG + chr4_2:51573107-51573126 Msa0605630:CDS 60.0%
!! GGTGGTGGTGGTGGATATGG+TGG + chr4_2:51573110-51573129 Msa0605630:CDS 60.0%
!! GGATATGGTGGAGAGCGTCG+TGG + chr4_2:51573122-51573141 Msa0605630:CDS 60.0%
!! GGTGGAGAGCGTCGTGGATA+TGG + chr4_2:51573128-51573147 Msa0605630:CDS 60.0%
!! GGAGAGCGTCGTGGATATGG+TGG + chr4_2:51573131-51573150 Msa0605630:CDS 60.0%
! CGTGGATATGGTGGAAGCGG+TGG + chr4_2:51573140-51573159 Msa0605630:CDS 60.0%
! GGATATGGTGGAAGCGGTGG+TGG + chr4_2:51573143-51573162 Msa0605630:CDS 60.0%
!! GGTGGAAGCGGTGGTGGATA+TGG + chr4_2:51573149-51573168 Msa0605630:CDS 60.0%
!! GGAAGCGGTGGTGGATATGG+TGG + chr4_2:51573152-51573171 Msa0605630:CDS 60.0%
!! AGCGGTGGTGGATATGGTGG+AGG + chr4_2:51573155-51573174 Msa0605630:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + chr4_2:51573158-51573177 Msa0605630:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_2:51573161-51573180 Msa0605630:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_2:51573164-51573183 Msa0605630:CDS 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG + chr4_2:51573170-51573189 Msa0605630:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + chr4_2:51573173-51573192 Msa0605630:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+TGG + chr4_2:51573176-51573195 Msa0605630:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr4_2:51573179-51573198 Msa0605630:CDS 60.0%
!! GGAGGTGGTGGATACAGCAG+AGG + chr4_2:51573215-51573234 Msa0605630:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_2:51573218-51573237 Msa0605630:CDS 60.0%
GGTGGATACAGCAGAGGAGG+CGG + chr4_2:51573221-51573240 Msa0605630:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+TGG + chr4_2:51573242-51573261 Msa0605630:CDS 60.0%
!! GGTGGAGGATATGGTGGTGG+TGG + chr4_2:51573245-51573264 Msa0605630:CDS 60.0%
!! GGTGGTGGTGGATACAGCAG+AGG + chr4_2:51573257-51573276 Msa0605630:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_2:51573218-51573237 Msa0605630:CDS 60.0%
GGTGGATACAGCAGAGGAGG+AGG + chr4_2:51573221-51573240 Msa0605630:CDS 60.0%
! AGCAGAGGAGGAGGTGATGG+TGG + chr4_2:51573272-51573291 Msa0605630:CDS 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_2:51572501-51572520 Msa0605630:CDS 60.0%
!! CTCGAGAGCATCGTTGTCGG+TGG - chr4_2:51572528-51572547 None:intergenic 60.0%
GTGAACCAAGCTCAGAGCCG+TGG + chr4_2:51573044-51573063 Msa0605630:CDS 60.0%
CAAGCTCAGAGCCGTGGTAG+CGG + chr4_2:51573050-51573069 Msa0605630:CDS 60.0%
CTCCATATCCACCGCCACCA+CGG - chr4_2:51573091-51573110 None:intergenic 60.0%
! GGTGGATATGGAGGTGGTGG+TGG + chr4_2:51573098-51573117 Msa0605630:CDS 60.0%
!! GGATATGGAGGTGGTGGTGG+TGG + chr4_2:51573101-51573120 Msa0605630:CDS 60.0%
!! GGAGGTGGTGGTGGTGGATA+TGG + chr4_2:51573107-51573126 Msa0605630:CDS 60.0%
!! GGTGGTGGTGGTGGATATGG+TGG + chr4_2:51573110-51573129 Msa0605630:CDS 60.0%
!! GGATATGGTGGAGAGCGTCG+TGG + chr4_2:51573122-51573141 Msa0605630:CDS 60.0%
!! GGTGGAGAGCGTCGTGGATA+TGG + chr4_2:51573128-51573147 Msa0605630:CDS 60.0%
!! GGAGAGCGTCGTGGATATGG+TGG + chr4_2:51573131-51573150 Msa0605630:CDS 60.0%
! CGTGGATATGGTGGAAGCGG+TGG + chr4_2:51573140-51573159 Msa0605630:CDS 60.0%
! GGATATGGTGGAAGCGGTGG+TGG + chr4_2:51573143-51573162 Msa0605630:CDS 60.0%
!! GGTGGAAGCGGTGGTGGATA+TGG + chr4_2:51573149-51573168 Msa0605630:CDS 60.0%
!! GGAAGCGGTGGTGGATATGG+TGG + chr4_2:51573152-51573171 Msa0605630:CDS 60.0%
!! AGCGGTGGTGGATATGGTGG+AGG + chr4_2:51573155-51573174 Msa0605630:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + chr4_2:51573158-51573177 Msa0605630:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_2:51573161-51573180 Msa0605630:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_2:51573164-51573183 Msa0605630:CDS 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG + chr4_2:51573170-51573189 Msa0605630:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + chr4_2:51573173-51573192 Msa0605630:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+TGG + chr4_2:51573176-51573195 Msa0605630:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr4_2:51573179-51573198 Msa0605630:CDS 60.0%
!! GGAGGTGGTGGATACAGCAG+AGG + chr4_2:51573215-51573234 Msa0605630:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_2:51573218-51573237 Msa0605630:CDS 60.0%
GGTGGATACAGCAGAGGAGG+CGG + chr4_2:51573221-51573240 Msa0605630:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+TGG + chr4_2:51573242-51573261 Msa0605630:CDS 60.0%
!! GGTGGAGGATATGGTGGTGG+TGG + chr4_2:51573245-51573264 Msa0605630:CDS 60.0%
!! GGTGGTGGTGGATACAGCAG+AGG + chr4_2:51573257-51573276 Msa0605630:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_2:51573218-51573237 Msa0605630:CDS 60.0%
GGTGGATACAGCAGAGGAGG+AGG + chr4_2:51573221-51573240 Msa0605630:CDS 60.0%
! AGCAGAGGAGGAGGTGATGG+TGG + chr4_2:51573272-51573291 Msa0605630:CDS 60.0%
!! CGCTACCACGGCTCTGAGCT+TGG - chr4_2:51573052-51573071 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+AGG + chr4_2:51573089-51573108 Msa0605630:CDS 65.0%
! GGTGGCGGTGGATATGGAGG+TGG + chr4_2:51573092-51573111 Msa0605630:CDS 65.0%
GGCGGTGGATATGGAGGTGG+TGG + chr4_2:51573095-51573114 Msa0605630:CDS 65.0%
ATCCACCACCTCCACCACGG+CGG - chr4_2:51573208-51573227 None:intergenic 65.0%
GGATACAGCAGAGGAGGCGG+TGG + chr4_2:51573224-51573243 Msa0605630:CDS 65.0%
TACAGCAGAGGAGGCGGTGG+TGG + chr4_2:51573227-51573246 Msa0605630:CDS 65.0%
!! GGAGGCGGTGGTGGAGGATA+TGG + chr4_2:51573236-51573255 Msa0605630:CDS 65.0%
!! GGCGGTGGTGGAGGATATGG+TGG + chr4_2:51573239-51573258 Msa0605630:CDS 65.0%
!! CGCTACCACGGCTCTGAGCT+TGG - chr4_2:51573052-51573071 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+AGG + chr4_2:51573089-51573108 Msa0605630:CDS 65.0%
! GGTGGCGGTGGATATGGAGG+TGG + chr4_2:51573092-51573111 Msa0605630:CDS 65.0%
GGCGGTGGATATGGAGGTGG+TGG + chr4_2:51573095-51573114 Msa0605630:CDS 65.0%
ATCCACCACCTCCACCACGG+CGG - chr4_2:51573208-51573227 None:intergenic 65.0%
GGATACAGCAGAGGAGGCGG+TGG + chr4_2:51573224-51573243 Msa0605630:CDS 65.0%
TACAGCAGAGGAGGCGGTGG+TGG + chr4_2:51573227-51573246 Msa0605630:CDS 65.0%
!! GGAGGCGGTGGTGGAGGATA+TGG + chr4_2:51573236-51573255 Msa0605630:CDS 65.0%
!! GGCGGTGGTGGAGGATATGG+TGG + chr4_2:51573239-51573258 Msa0605630:CDS 65.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_2:51573053-51573072 Msa0605630:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_2:51573056-51573075 Msa0605630:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+CGG + chr4_2:51573059-51573078 Msa0605630:CDS 70.0%
CTCCGCCACCACCGCTACCA+CGG - chr4_2:51573064-51573083 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_2:51573086-51573105 Msa0605630:CDS 70.0%
! AGCAGAGGAGGCGGTGGTGG+AGG + chr4_2:51573230-51573249 Msa0605630:CDS 70.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_2:51573053-51573072 Msa0605630:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_2:51573056-51573075 Msa0605630:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+CGG + chr4_2:51573059-51573078 Msa0605630:CDS 70.0%
CTCCGCCACCACCGCTACCA+CGG - chr4_2:51573064-51573083 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_2:51573086-51573105 Msa0605630:CDS 70.0%
! AGCAGAGGAGGCGGTGGTGG+AGG + chr4_2:51573230-51573249 Msa0605630:CDS 70.0%
!! CGTGGTAGCGGTGGTGGCGG+AGG + chr4_2:51573062-51573081 Msa0605630:CDS 75.0%
!! GGTAGCGGTGGTGGCGGAGG+AGG + chr4_2:51573065-51573084 Msa0605630:CDS 75.0%
!! GGTGGTGGTGGTGACCGCCG+TGG + chr4_2:51573191-51573210 Msa0605630:CDS 75.0%
! GGTGGTGGTGACCGCCGTGG+TGG + chr4_2:51573194-51573213 Msa0605630:CDS 75.0%
GGTGGTGACCGCCGTGGTGG+AGG + chr4_2:51573197-51573216 Msa0605630:CDS 75.0%
! GGTGACCGCCGTGGTGGAGG+TGG + chr4_2:51573200-51573219 Msa0605630:CDS 75.0%
! GACCGCCGTGGTGGAGGTGG+TGG + chr4_2:51573203-51573222 Msa0605630:CDS 75.0%
!! CGTGGTAGCGGTGGTGGCGG+AGG + chr4_2:51573062-51573081 Msa0605630:CDS 75.0%
!! GGTAGCGGTGGTGGCGGAGG+AGG + chr4_2:51573065-51573084 Msa0605630:CDS 75.0%
!! GGTGGTGGTGGTGACCGCCG+TGG + chr4_2:51573191-51573210 Msa0605630:CDS 75.0%
! GGTGGTGGTGACCGCCGTGG+TGG + chr4_2:51573194-51573213 Msa0605630:CDS 75.0%
GGTGGTGACCGCCGTGGTGG+AGG + chr4_2:51573197-51573216 Msa0605630:CDS 75.0%
! GGTGACCGCCGTGGTGGAGG+TGG + chr4_2:51573200-51573219 Msa0605630:CDS 75.0%
! GACCGCCGTGGTGGAGGTGG+TGG + chr4_2:51573203-51573222 Msa0605630:CDS 75.0%
!!! GGTGGTGGCGGAGGAGGCCG+TGG + chr4_2:51573071-51573090 Msa0605630:CDS 80.0%
!!! GGTGGCGGAGGAGGCCGTGG+TGG + chr4_2:51573074-51573093 Msa0605630:CDS 80.0%
! GGCGGAGGAGGCCGTGGTGG+CGG + chr4_2:51573077-51573096 Msa0605630:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_2:51573080-51573099 Msa0605630:CDS 80.0%
!!! GGTGGTGGCGGAGGAGGCCG+TGG + chr4_2:51573071-51573090 Msa0605630:CDS 80.0%
!!! GGTGGCGGAGGAGGCCGTGG+TGG + chr4_2:51573074-51573093 Msa0605630:CDS 80.0%
! GGCGGAGGAGGCCGTGGTGG+CGG + chr4_2:51573077-51573096 Msa0605630:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_2:51573080-51573099 Msa0605630:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51572402 51573565 51572402 ID=Msa0605630;Name=Msa0605630
chr4_2 mRNA 51572402 51573565 51572402 ID=Msa0605630-mRNA-1;Parent=Msa0605630;Name=Msa0605630-mRNA-1;_AED=0.29;_eAED=0.29;_QI=77|1|1|1|1|1|2|264|163
chr4_2 exon 51572402 51572586 51572402 ID=Msa0605630-mRNA-1:exon:12360;Parent=Msa0605630-mRNA-1
chr4_2 exon 51572918 51573565 51572918 ID=Msa0605630-mRNA-1:exon:12361;Parent=Msa0605630-mRNA-1
chr4_2 five_prime_UTR 51572402 51572478 51572402 ID=Msa0605630-mRNA-1:five_prime_utr;Parent=Msa0605630-mRNA-1
chr4_2 CDS 51572479 51572586 51572479 ID=Msa0605630-mRNA-1:cds;Parent=Msa0605630-mRNA-1
chr4_2 CDS 51572918 51573301 51572918 ID=Msa0605630-mRNA-1:cds;Parent=Msa0605630-mRNA-1
chr4_2 three_prime_UTR 51573302 51573565 51573302 ID=Msa0605630-mRNA-1:three_prime_utr;Parent=Msa0605630-mRNA-1
Gene Sequence

>Msa0605630

ATGGCTGATGTTGAATACCGATGCTTCGTCGGAGGTCTTGCATGGGCCACCGACAACGATGCTCTCGAGAAAGCCTTCTCTCAATACGGCGAGATCGTTGATTCCAAGATCATTAACGATCGTGAAACTGGACGATCAAGAGGTTTTGGATTTGTGACATTCGCTAACGAGAAATCCATGAACGATGCCATTGAAGCAATGAACGGTCAGGATTTGGATGGACGTAACATCACTGTGAACCAAGCTCAGAGCCGTGGTAGCGGTGGTGGCGGAGGAGGCCGTGGTGGCGGTGGATATGGAGGTGGTGGTGGTGGATATGGTGGAGAGCGTCGTGGATATGGTGGAAGCGGTGGTGGATATGGTGGAGGAGGTGGTGGATATGGTGGTGGTGGTGACCGCCGTGGTGGAGGTGGTGGATACAGCAGAGGAGGCGGTGGTGGAGGATATGGTGGTGGTGGATACAGCAGAGGAGGAGGTGATGGTGGTTATTAG
Protein sequence

>Msa0605630

MADVEYRCFVGGLAWATDNDALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIEAMNGQDLDGRNITVNQAQSRGSGGGGGGRGGGGYGGGGGGYGGERRGYGGSGGGYGGGGGGYGGGGDRRGGGGGYSRGGGGGGYGGGGYSRGGGDGGY*