AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605640 MtrunA17_Chr4g0035031 96.842 190 5 1 1 189 1 190 1.78e-108 308
Msa0605640 MtrunA17_Chr4g0035051 88.235 85 10 0 1 85 1 85 1.54e-52 164
Msa0605640 MtrunA17_Chr4g0035001 85.556 90 13 0 4 93 2 91 1.80e-47 152
Msa0605640 MtrunA17_Chr2g0278661 56.180 89 38 1 1 89 1 88 1.73e-31 114
Msa0605640 MtrunA17_Chr4g0050781 44.231 156 82 2 3 158 2 152 2.00e-24 95.1
Msa0605640 MtrunA17_Chr3g0086861 47.059 85 45 0 3 87 39 123 7.05e-23 94.7
Msa0605640 MtrunA17_Chr3g0116631 51.852 81 39 0 8 88 206 286 7.80e-23 92.8
Msa0605640 MtrunA17_Chr3g0116631 27.815 151 99 1 9 159 110 250 4.06e-12 63.2
Msa0605640 MtrunA17_Chr4g0061451 47.500 80 42 0 9 88 43 122 2.41e-22 90.9
Msa0605640 MtrunA17_Chr7g0219021 44.578 83 46 0 9 91 41 123 5.93e-22 89.7
Msa0605640 MtrunA17_Chr3g0121631 50.633 79 38 1 9 87 37 114 1.18e-20 83.6
Msa0605640 MtrunA17_Chr4g0058151 50.617 81 40 0 8 88 216 296 1.44e-20 86.7
Msa0605640 MtrunA17_Chr4g0058151 30.263 152 93 2 8 159 122 260 2.25e-14 69.7
Msa0605640 MtrunA17_Chr1g0181111 50.575 87 43 0 2 88 190 276 1.85e-18 80.9
Msa0605640 MtrunA17_Chr1g0181111 36.283 113 71 1 9 120 85 197 1.51e-11 61.6
Msa0605640 MtrunA17_Chr3g0119311 40.244 82 49 0 9 90 37 118 2.05e-18 77.4
Msa0605640 MtrunA17_Chr7g0266751 40.741 81 48 0 6 86 30 110 3.22e-17 74.3
Msa0605640 MtrunA17_Chr3g0144311 46.053 76 40 1 9 84 30 104 1.95e-16 75.1
Msa0605640 MtrunA17_Chr4g0017401 41.000 100 52 4 5 99 12 109 2.90e-16 74.7
Msa0605640 MtrunA17_Chr1g0168601 41.463 82 48 0 4 85 149 230 1.65e-15 72.0
Msa0605640 MtrunA17_Chr5g0447761 37.975 79 47 1 9 87 7 83 2.15e-15 73.6
Msa0605640 MtrunA17_Chr5g0447761 29.605 152 89 3 9 150 107 250 3.82e-11 61.2
Msa0605640 MtrunA17_Chr3g0101061 37.975 79 47 1 9 87 41 117 3.34e-15 72.8
Msa0605640 MtrunA17_Chr3g0101061 31.757 148 87 4 9 143 141 287 5.88e-11 60.5
Msa0605640 MtrunA17_Chr7g0276531 46.053 76 40 1 9 84 19 93 4.99e-15 70.9
Msa0605640 MtrunA17_Chr2g0327921 39.024 82 50 0 9 90 8 89 7.50e-15 67.0
Msa0605640 MtrunA17_Chr3g0127751 45.000 80 43 1 9 88 27 105 7.94e-15 70.5
Msa0605640 MtrunA17_Chr5g0426311 44.737 76 41 1 9 84 17 91 8.48e-15 70.9
Msa0605640 MtrunA17_Chr2g0290191 39.241 79 46 1 9 87 5 81 9.15e-15 71.6
Msa0605640 MtrunA17_Chr2g0290191 33.835 133 78 3 9 131 107 239 1.39e-12 65.1
Msa0605640 MtrunA17_Chr3g0120721 32.374 139 86 1 9 147 107 237 1.84e-14 70.5
Msa0605640 MtrunA17_Chr3g0120721 36.585 82 50 1 9 90 19 98 1.79e-13 67.4
Msa0605640 MtrunA17_Chr1g0186351 35.443 79 49 1 9 87 7 83 1.91e-14 70.5
Msa0605640 MtrunA17_Chr1g0186351 30.097 206 113 5 9 184 113 317 8.67e-11 60.1
Msa0605640 MtrunA17_Chr8g0346011 44.737 76 41 1 9 84 38 112 2.37e-14 69.7
Msa0605640 MtrunA17_Chr4g0065741 37.500 96 57 1 1 96 67 159 3.44e-14 67.0
Msa0605640 MtrunA17_Chr5g0406171 32.955 176 98 5 10 171 133 302 4.80e-14 68.9
Msa0605640 MtrunA17_Chr6g0449481 38.095 84 50 1 9 92 46 127 1.01e-13 68.6
Msa0605640 MtrunA17_Chr6g0449481 33.858 127 80 1 9 131 134 260 1.99e-11 61.6
Msa0605640 MtrunA17_Chr7g0263001 37.931 87 54 0 1 87 28 114 2.24e-13 64.3
Msa0605640 MtrunA17_Chr7g0219031 42.424 66 38 0 9 74 41 106 3.26e-13 63.2
Msa0605640 MtrunA17_Chr6g0481071 38.750 80 49 0 9 88 96 175 8.24e-13 63.9
Msa0605640 MtrunA17_Chr4g0042161 38.298 94 52 2 11 101 54 144 1.59e-12 62.0
Msa0605640 MtrunA17_Chr5g0402191 41.250 80 43 2 9 87 5 81 2.34e-12 64.3
Msa0605640 MtrunA17_Chr4g0062931 35.088 171 90 8 10 179 9 159 5.32e-12 63.5
Msa0605640 MtrunA17_Chr4g0018581 44.444 72 40 0 10 81 453 524 7.05e-12 63.5
Msa0605640 MtrunA17_Chr4g0031091 39.726 73 44 0 9 81 34 106 8.04e-12 59.3
Msa0605640 MtrunA17_Chr1g0198431 35.870 92 56 1 9 97 193 284 9.22e-12 62.4
Msa0605640 MtrunA17_Chr3g0110211 39.175 97 58 1 3 98 107 203 1.51e-11 62.0
Msa0605640 MtrunA17_Chr3g0135111 31.532 111 75 1 9 119 118 227 1.93e-11 61.6
Msa0605640 MtrunA17_Chr5g0419351 36.957 92 58 0 10 101 12 103 2.54e-11 59.7
Msa0605640 MtrunA17_Chr3g0088511 38.356 73 45 0 9 81 33 105 5.80e-11 57.8
Msa0605640 MtrunA17_Chr8g0360551 35.955 89 55 2 9 97 74 160 6.64e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605640 AT4G39260.3 77.778 90 20 0 4 93 2 91 6.43e-48 151
Msa0605640 AT2G21660.2 78.824 85 18 0 1 85 1 85 4.65e-45 146
Msa0605640 AT2G21660.1 78.824 85 18 0 1 85 1 85 7.76e-45 146
Msa0605640 AT4G39260.1 75.610 82 20 0 4 85 2 83 2.99e-42 140
Msa0605640 AT4G39260.2 71.171 111 29 1 4 114 2 109 1.99e-38 129
Msa0605640 AT4G39260.4 76.119 67 16 0 4 70 2 68 4.66e-34 117
Msa0605640 AT3G26420.1 54.167 96 43 1 3 98 2 96 4.08e-31 113
Msa0605640 AT4G13850.2 54.217 83 38 0 9 91 36 118 3.41e-25 95.9
Msa0605640 AT4G13850.1 55.208 96 43 0 9 104 36 131 7.90e-24 92.4
Msa0605640 AT3G23830.2 52.475 101 43 1 9 109 36 131 8.12e-24 91.7
Msa0605640 AT3G23830.1 52.475 101 43 1 9 109 36 131 8.12e-24 91.7
Msa0605640 AT5G04280.1 47.475 99 47 1 9 102 8 106 1.17e-23 95.5
Msa0605640 AT4G13850.3 56.566 99 43 0 9 107 36 134 1.26e-23 91.7
Msa0605640 AT4G13850.4 56.383 94 40 1 9 101 36 129 3.60e-23 90.1
Msa0605640 AT5G61030.2 49.367 79 40 0 9 87 41 119 2.01e-22 92.0
Msa0605640 AT5G61030.1 49.367 79 40 0 9 87 41 119 2.01e-22 92.0
Msa0605640 AT2G21690.1 50.000 90 40 1 3 87 2 91 2.69e-21 84.7
Msa0605640 AT4G24770.1 47.561 82 43 0 8 89 244 325 1.48e-20 87.4
Msa0605640 AT4G24770.1 45.783 83 45 0 7 89 149 231 7.52e-16 74.3
Msa0605640 AT4G24770.2 47.561 82 43 0 8 89 244 325 1.48e-20 87.4
Msa0605640 AT4G24770.2 45.783 83 45 0 7 89 149 231 7.52e-16 74.3
Msa0605640 AT5G06210.1 44.318 88 49 0 6 93 32 119 1.61e-20 83.6
Msa0605640 AT5G50250.1 48.193 83 43 0 5 87 204 286 9.07e-20 84.7
Msa0605640 AT5G50250.1 32.680 153 90 2 7 159 112 251 3.09e-16 75.1
Msa0605640 AT3G53460.1 45.783 83 45 0 9 91 258 340 1.72e-19 84.7
Msa0605640 AT3G53460.3 45.783 83 45 0 9 91 258 340 1.72e-19 84.7
Msa0605640 AT3G53460.2 45.783 83 45 0 9 91 258 340 1.72e-19 84.7
Msa0605640 AT2G37220.1 45.679 81 44 0 9 89 205 285 2.31e-19 83.6
Msa0605640 AT3G53460.4 45.783 83 45 0 9 91 279 361 2.53e-19 84.3
Msa0605640 AT1G60650.2 42.169 83 48 0 5 87 9 91 3.92e-19 83.2
Msa0605640 AT1G60650.1 42.169 83 48 0 5 87 9 91 3.92e-19 83.2
Msa0605640 AT1G74230.1 54.118 85 38 1 9 93 35 118 6.23e-19 82.4
Msa0605640 AT3G07810.1 41.772 79 44 1 9 87 7 83 3.18e-17 79.0
Msa0605640 AT3G07810.1 33.577 137 73 4 9 131 109 241 4.36e-12 64.3
Msa0605640 AT3G07810.2 41.772 79 44 1 9 87 7 83 3.18e-17 79.0
Msa0605640 AT3G07810.2 33.577 137 73 4 9 131 109 241 4.32e-12 64.3
Msa0605640 AT1G76460.1 48.684 76 38 1 9 84 25 99 4.67e-17 77.4
Msa0605640 AT1G22330.1 44.118 102 51 3 9 105 18 118 7.65e-17 77.0
Msa0605640 AT1G18630.1 46.154 78 42 0 9 86 37 114 1.33e-16 73.6
Msa0605640 AT5G47320.1 46.753 77 41 0 9 85 32 108 1.67e-16 74.7
Msa0605640 AT4G26650.1 34.286 105 67 1 9 113 16 118 2.36e-16 76.6
Msa0605640 AT4G26650.2 34.286 105 67 1 9 113 13 115 2.49e-16 76.6
Msa0605640 AT4G14300.1 40.506 79 45 1 9 87 7 83 3.27e-16 75.9
Msa0605640 AT4G14300.2 40.506 79 45 1 9 87 7 83 3.27e-16 75.9
Msa0605640 AT1G20880.2 46.053 76 40 1 9 84 25 99 5.54e-16 74.3
Msa0605640 AT1G20880.3 46.053 76 40 1 9 84 25 99 5.54e-16 74.3
Msa0605640 AT1G20880.1 46.053 76 40 1 9 84 25 99 5.54e-16 74.3
Msa0605640 AT3G46020.1 42.857 77 44 0 9 85 8 84 1.78e-15 69.3
Msa0605640 AT1G22910.1 40.404 99 54 3 5 99 9 106 1.82e-15 72.4
Msa0605640 AT2G46780.1 46.053 76 40 1 9 84 23 97 2.21e-15 73.2
Msa0605640 AT2G46780.2 46.053 76 40 1 9 84 23 97 2.21e-15 73.2
Msa0605640 AT1G22910.2 40.404 99 54 3 5 99 9 106 2.27e-15 72.0
Msa0605640 AT2G33410.1 39.241 79 46 1 9 87 7 83 2.85e-15 73.2
Msa0605640 AT3G13224.3 38.462 91 50 2 6 92 4 92 3.71e-15 72.8
Msa0605640 AT3G13224.3 32.955 88 58 1 3 89 94 181 9.19e-12 63.2
Msa0605640 AT3G13224.2 39.286 84 49 1 9 92 20 101 5.10e-15 72.4
Msa0605640 AT3G13224.2 32.955 88 58 1 3 89 103 190 3.23e-12 64.3
Msa0605640 AT1G33470.3 43.421 76 42 1 9 84 8 82 5.30e-15 71.2
Msa0605640 AT5G55550.5 33.981 103 66 1 9 111 7 107 6.22e-15 72.4
Msa0605640 AT5G55550.4 33.981 103 66 1 9 111 7 107 6.22e-15 72.4
Msa0605640 AT5G55550.9 33.981 103 66 1 9 111 7 107 6.22e-15 72.4
Msa0605640 AT5G55550.7 33.981 103 66 1 9 111 7 107 6.22e-15 72.4
Msa0605640 AT5G55550.1 33.981 103 66 1 9 111 7 107 6.22e-15 72.4
Msa0605640 AT1G33470.2 43.421 76 42 1 9 84 8 82 6.49e-15 70.9
Msa0605640 AT1G33470.1 43.421 76 42 1 9 84 8 82 6.57e-15 70.9
Msa0605640 AT5G55550.2 33.981 103 66 1 9 111 7 107 7.09e-15 72.4
Msa0605640 AT5G55550.3 33.981 103 66 1 9 111 7 107 7.09e-15 72.4
Msa0605640 AT5G55550.6 33.981 103 66 1 9 111 7 107 7.09e-15 72.4
Msa0605640 AT5G55550.11 33.981 103 66 1 9 111 7 107 7.09e-15 72.4
Msa0605640 AT3G08000.1 43.678 87 43 2 9 92 42 125 1.18e-14 68.2
Msa0605640 AT1G17640.2 42.500 80 44 1 9 88 67 144 1.29e-14 71.2
Msa0605640 AT1G17640.1 42.500 80 44 1 9 88 67 144 1.29e-14 71.2
Msa0605640 AT1G17640.3 42.500 80 44 1 9 88 67 144 1.29e-14 71.2
Msa0605640 AT1G22910.3 40.404 99 54 3 5 99 9 106 1.65e-14 70.9
Msa0605640 AT2G37510.1 45.946 74 40 0 9 82 35 108 2.05e-14 67.4
Msa0605640 AT5G59865.1 44.304 79 44 0 11 89 29 107 3.33e-14 66.2
Msa0605640 AT1G78260.1 43.210 81 44 2 5 84 13 92 5.39e-14 68.9
Msa0605640 AT5G19960.1 36.134 119 64 3 10 127 9 116 6.18e-14 69.3
Msa0605640 AT3G54770.3 43.421 76 42 1 9 84 18 92 6.27e-14 68.6
Msa0605640 AT1G78260.2 43.421 76 42 1 9 84 18 92 7.46e-14 68.6
Msa0605640 AT3G54770.1 43.421 76 42 1 9 84 18 92 7.52e-14 68.2
Msa0605640 AT5G53720.1 42.683 82 46 1 3 84 4 84 2.32e-13 63.5
Msa0605640 AT1G60000.1 40.244 82 49 0 4 85 173 254 3.54e-13 66.2
Msa0605640 AT5G40490.1 38.542 96 57 1 9 104 43 136 4.86e-13 67.0
Msa0605640 AT3G13224.1 39.286 84 49 1 9 92 20 101 5.12e-13 65.5
Msa0605640 AT5G55550.8 40.278 72 41 1 9 80 7 76 5.26e-13 67.0
Msa0605640 AT2G27330.1 29.670 91 63 1 11 100 24 114 5.71e-13 63.2
Msa0605640 AT5G55550.10 40.278 72 41 1 9 80 7 76 6.17e-13 66.6
Msa0605640 AT3G14100.1 42.308 78 45 0 7 84 143 220 6.45e-13 66.6
Msa0605640 AT2G14160.1 39.286 84 32 2 1 66 1 83 8.85e-13 61.6
Msa0605640 AT5G47620.1 36.709 79 48 1 9 87 7 83 9.15e-13 66.2
Msa0605640 AT5G47620.2 36.709 79 48 1 9 87 7 83 9.15e-13 66.2
Msa0605640 AT5G47620.5 36.709 79 48 1 9 87 7 83 9.15e-13 66.2
Msa0605640 AT4G13860.1 40.541 74 44 0 9 82 4 77 1.16e-12 61.2
Msa0605640 AT1G01080.1 41.772 79 41 2 7 83 211 286 1.85e-12 64.7
Msa0605640 AT1G01080.2 41.772 79 41 2 7 83 212 287 1.98e-12 64.7
Msa0605640 AT1G54080.1 42.308 78 45 0 7 84 147 224 2.81e-12 64.7
Msa0605640 AT5G53680.1 46.377 69 36 1 9 77 14 81 5.88e-12 61.6
Msa0605640 AT5G04280.3 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0605640 AT5G04280.4 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0605640 AT5G04280.5 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0605640 AT5G04280.2 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0605640 AT5G47620.4 37.500 72 43 1 9 80 7 76 1.22e-11 62.8
Msa0605640 AT4G27000.1 43.243 74 41 1 7 79 172 245 2.24e-11 62.0
Msa0605640 AT4G36960.1 36.782 87 53 1 6 90 1 87 3.66e-11 61.2
Msa0605640 AT4G36960.2 36.782 87 53 1 6 90 1 87 3.66e-11 61.2
Msa0605640 AT5G51300.1 41.333 75 44 0 7 81 479 553 3.93e-11 61.6
Msa0605640 AT5G51300.2 41.333 75 44 0 7 81 479 553 3.93e-11 61.6
Msa0605640 AT5G51300.3 41.333 75 44 0 7 81 479 553 3.93e-11 61.6
Msa0605640 AT3G20930.1 35.000 80 52 0 9 88 283 362 4.52e-11 61.2
Msa0605640 AT3G06970.1 30.973 113 71 3 9 120 13 119 7.74e-11 60.1
Msa0605640 AT2G22090.2 38.043 92 54 3 5 95 100 189 8.10e-11 60.5
Msa0605640 AT2G22090.1 38.043 92 54 3 5 95 100 189 8.58e-11 60.1

Find 100 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACCCATCGAACTATT+AGG 0.243068 4_2:-51582939 None:intergenic
ACTGGAAGATCAAGAGGTTT+TGG 0.244628 4_2:+51583329 Msa0605640:CDS
GGTGGAGTTGGTGGTGGTTA+TGG 0.247285 4_2:+51583623 Msa0605640:CDS
GGAGGTGGCGGTGGTGGATA+TGG 0.255891 4_2:+51583518 Msa0605640:CDS
TATGGTGGCGGTGGTGGTTA+TGG 0.272650 4_2:+51583497 Msa0605640:CDS
AGTGGTGGTGGTGGAGGATA+TGG 0.275624 4_2:+51583578 Msa0605640:CDS
GGAGGTGGTGATCGTGGATA+TGG 0.294183 4_2:+51583602 Msa0605640:CDS
GGTGGCGGCGGTGGTGGATA+TGG 0.308829 4_2:+51583479 Msa0605640:CDS
TTGGTTGAAGTTTAGTGATT+TGG 0.311002 4_2:+51583834 Msa0605640:three_prime_UTR
AAGAACCCATCGAACTATTA+GGG 0.328265 4_2:-51582938 None:intergenic
GTTATCAATGATCGTGAAAC+TGG 0.330573 4_2:+51583311 Msa0605640:CDS
GGTGGCGGTGGTGGATATGG+TGG 0.346314 4_2:+51583521 Msa0605640:CDS
GGAGGTGGTGACCGTGGTTA+TGG 0.350450 4_2:+51583677 Msa0605640:CDS
GCTTCGTCGGTGGTCTTGCT+TGG 0.353153 4_2:+51582979 Msa0605640:CDS
GGAGGTAGAGGTGGCGGCGG+TGG 0.353721 4_2:+51583470 Msa0605640:CDS
GTGCTGATAGCGGTGGAAAC+TGG 0.354817 4_2:+51583735 Msa0605640:CDS
GTTTATACAAAACCAGATTC+AGG 0.358098 4_2:-51583903 None:intergenic
AGTGGTAGGGACCGTGGATA+TGG 0.363432 4_2:+51583650 Msa0605640:CDS
GAGCAAGCCTTCTCTAAGTT+TGG 0.374142 4_2:+51583023 Msa0605640:CDS
GGTAGAGGTGGCGGCGGTGG+TGG 0.374533 4_2:+51583473 Msa0605640:CDS
GGTTATGGAGGTGGCGGTGG+TGG 0.376259 4_2:+51583512 Msa0605640:CDS
CTTCGTCGGTGGTCTTGCTT+GGG 0.385202 4_2:+51582980 Msa0605640:CDS
GGTGGTTATGGAGGTGGCGG+TGG 0.399782 4_2:+51583509 Msa0605640:CDS
GTGGTTATGGTGGAAGTGGT+AGG 0.399799 4_2:+51583636 Msa0605640:CDS
TTGATTTAGTTTGATTTAGT+TGG 0.405201 4_2:+51583815 Msa0605640:three_prime_UTR
CGTGGATATGGTGGTGGAGG+TGG 0.410093 4_2:+51583662 Msa0605640:CDS
ACTGGAGGGATTAGAAAGTT+AGG 0.417767 4_2:+51583753 Msa0605640:exon
GGCGGTGGTGGATATGGTGG+AGG 0.424937 4_2:+51583524 Msa0605640:CDS
GGAGTTGGTGGTGGTTATGG+TGG 0.432658 4_2:+51583626 Msa0605640:CDS
GGCGGTGGTGGTTATGGAGG+TGG 0.433094 4_2:+51583503 Msa0605640:CDS
GGTGGATATGGTGGCGGTGG+TGG 0.437015 4_2:+51583491 Msa0605640:CDS
CAGTCACGTGGCAGTGGTGG+AGG 0.442750 4_2:+51583449 Msa0605640:CDS
AGAAAGTTAGGTCTTCAGTT+TGG 0.445794 4_2:+51583765 Msa0605640:exon
GGCAGTGGTGGAGGAGGTAG+AGG 0.451811 4_2:+51583458 Msa0605640:CDS
GGTGGTGGAGGATATGGTGG+AGG 0.455686 4_2:+51583584 Msa0605640:CDS
GGTGGTGGTTATGGTGGAAG+TGG 0.459200 4_2:+51583632 Msa0605640:CDS
GGTGGTGGATATGGTGGAGG+TGG 0.459945 4_2:+51583527 Msa0605640:CDS
GGTGGATATGGTGGAGGTGG+TGG 0.461684 4_2:+51583530 Msa0605640:CDS
GGCGGCGGTGGTGGATATGG+TGG 0.462700 4_2:+51583482 Msa0605640:CDS
GTATAAACGATTGATTGAAA+AGG 0.465420 4_2:+51583918 Msa0605640:three_prime_UTR
CGTGGATATGGTGGAGTTGG+TGG 0.466298 4_2:+51583614 Msa0605640:CDS
GGAGGTGGTGGTCGTCGTGA+TGG 0.466320 4_2:+51583542 Msa0605640:CDS
GGCCACTGATAGCCAAGCTC+TGG 0.466514 4_2:+51583001 Msa0605640:CDS
GGAGGAGGTAGAGGTGGCGG+CGG 0.469756 4_2:+51583467 Msa0605640:CDS
AGCAGAAGTGGTGGTGGTGG+AGG 0.470138 4_2:+51583572 Msa0605640:CDS
CGTGGTTATGGTGGTGGTGG+CGG 0.475722 4_2:+51583689 Msa0605640:CDS
AGAAGGCTTGCTCCAGAGCT+TGG 0.478074 4_2:-51583013 None:intergenic
GGTGGAGGATATGGTGGAGG+TGG 0.483928 4_2:+51583587 Msa0605640:CDS
GATCGTGGATATGGTGGAGT+TGG 0.492129 4_2:+51583611 Msa0605640:CDS
GGATATGGTGGAGTTGGTGG+TGG 0.492858 4_2:+51583617 Msa0605640:CDS
GGTGGTGGTGGAGGATATGG+TGG 0.497601 4_2:+51583581 Msa0605640:CDS
TGGTTATGGTGGAAGTGGTA+GGG 0.499432 4_2:+51583637 Msa0605640:CDS
AAGTTAGGTCTTCAGTTTGG+TGG 0.499779 4_2:+51583768 Msa0605640:three_prime_UTR
GGCGGTGGTGGATATGGTGG+CGG 0.500481 4_2:+51583485 Msa0605640:CDS
TACAGCAGAAGTGGTGGTGG+TGG 0.500718 4_2:+51583569 Msa0605640:CDS
GGTGGCGGTGGTGGTTATGG+AGG 0.505677 4_2:+51583500 Msa0605640:CDS
CAAGACCACCGACGAAGCAC+CGG 0.514431 4_2:-51582974 None:intergenic
GACCGTGGTTATGGTGGTGG+TGG 0.516242 4_2:+51583686 Msa0605640:CDS
GATCGCTACTCTCGCGGTGG+TGG 0.518871 4_2:+51583713 Msa0605640:CDS
GGTGGTGGATATGGTGGCGG+TGG 0.523108 4_2:+51583488 Msa0605640:CDS
GGTGGTGATCGTGGATATGG+TGG 0.523375 4_2:+51583605 Msa0605640:CDS
GCTCAGTCACGTGGCAGTGG+TGG 0.524094 4_2:+51583446 Msa0605640:CDS
GGTGGTGGTTATGGAGGTGG+CGG 0.530702 4_2:+51583506 Msa0605640:CDS
GGTGGAGGAGGTAGAGGTGG+CGG 0.531842 4_2:+51583464 Msa0605640:CDS
TCACGTGGCAGTGGTGGAGG+AGG 0.534421 4_2:+51583452 Msa0605640:CDS
TATGGTGGAGGTGGTGATCG+TGG 0.539028 4_2:+51583596 Msa0605640:CDS
GAAGCTCAGTCACGTGGCAG+TGG 0.541791 4_2:+51583443 Msa0605640:CDS
GACCGTGGATATGGTGGTGG+AGG 0.547656 4_2:+51583659 Msa0605640:CDS
AGGGACCGTGGATATGGTGG+TGG 0.548183 4_2:+51583656 Msa0605640:CDS
AATTGAAGAGATGAACGGTC+AGG 0.551398 4_2:+51583391 Msa0605640:CDS
TGGTGAAATCACTGATTCCA+AGG 0.553599 4_2:+51583043 Msa0605640:CDS
GGTGACCGTGGTTATGGTGG+TGG 0.556613 4_2:+51583683 Msa0605640:CDS
GGTGGTGGTCGTCGTGATGG+TGG 0.559431 4_2:+51583545 Msa0605640:CDS
GGCGGTGATCGCTACTCTCG+CGG 0.560534 4_2:+51583707 Msa0605640:CDS
CGTGAAACTGGAAGATCAAG+AGG 0.562850 4_2:+51583323 Msa0605640:CDS
CTCCAGAGCTTGGCTATCAG+TGG 0.564700 4_2:-51583003 None:intergenic
AGTGGTGGAGGAGGTAGAGG+TGG 0.566280 4_2:+51583461 Msa0605640:CDS
CGCGGTGGTGGTGCTGATAG+CGG 0.567365 4_2:+51583725 Msa0605640:CDS
GGTGGTGACCGTGGTTATGG+TGG 0.575688 4_2:+51583680 Msa0605640:CDS
GATTTCACCAAACTTAGAGA+AGG 0.575852 4_2:-51583030 None:intergenic
GGATACAGCAGAAGTGGTGG+TGG 0.583391 4_2:+51583566 Msa0605640:CDS
GTTGAGTACCGGTGCTTCGT+CGG 0.588309 4_2:+51582966 Msa0605640:CDS
GGTAGGGACCGTGGATATGG+TGG 0.594368 4_2:+51583653 Msa0605640:CDS
CTGATAGCGGTGGAAACTGG+AGG 0.605217 4_2:+51583738 Msa0605640:CDS
ATGAACGGTCAGGATATTGA+CGG 0.611489 4_2:+51583401 Msa0605640:CDS
GAGTACCGGTGCTTCGTCGG+TGG 0.614835 4_2:+51582969 Msa0605640:CDS
ACGGTCAGGATATTGACGGC+AGG 0.621480 4_2:+51583405 Msa0605640:CDS
GTTCTTCTGATGTTGAGTAC+CGG 0.622558 4_2:+51582955 Msa0605640:CDS
GGTGGAAGTGGTAGGGACCG+TGG 0.623581 4_2:+51583644 Msa0605640:CDS
GGTGGTGGAGGTGGTGACCG+TGG 0.625086 4_2:+51583671 Msa0605640:CDS
CACCTCCACCACCATATCCA+CGG 0.630646 4_2:-51583661 None:intergenic
TGATAGCGGTGGAAACTGGA+GGG 0.650756 4_2:+51583739 Msa0605640:CDS
AGAACCCATCGAACTATTAG+GGG 0.657602 4_2:-51582937 None:intergenic
CGCCACCACCACCATAACCA+CGG 0.664909 4_2:-51583688 None:intergenic
GATGGTGGATACAGCAGAAG+TGG 0.665118 4_2:+51583560 Msa0605640:CDS
GTGAATGAAGCTCAGTCACG+TGG 0.667871 4_2:+51583437 Msa0605640:CDS
GATGCAATTGAAGAGATGAA+CGG 0.671848 4_2:+51583386 Msa0605640:CDS
GGTGATCGCTACTCTCGCGG+TGG 0.687217 4_2:+51583710 Msa0605640:CDS
GGTGGATACAGCAGAAGTGG+TGG 0.687566 4_2:+51583563 Msa0605640:CDS
GGTGGTGGTGCTGATAGCGG+TGG 0.706620 4_2:+51583728 Msa0605640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTTAGTTTGATTTAGT+TGG + chr4_2:51583815-51583834 Msa0605640:three_prime_UTR 20.0%
!! TGAAAAGGATAATGAAAAAT+TGG + chr4_2:51583933-51583952 Msa0605640:three_prime_UTR 20.0%
!!! TTGATTTAGTTTGATTTAGT+TGG + chr4_2:51583815-51583834 Msa0605640:three_prime_UTR 20.0%
!! TGAAAAGGATAATGAAAAAT+TGG + chr4_2:51583933-51583952 Msa0605640:three_prime_UTR 20.0%
! GTATAAACGATTGATTGAAA+AGG + chr4_2:51583918-51583937 Msa0605640:three_prime_UTR 25.0%
! GTATAAACGATTGATTGAAA+AGG + chr4_2:51583918-51583937 Msa0605640:three_prime_UTR 25.0%
ATTAGGGGAAAAAAAGATGA+AGG - chr4_2:51582925-51582944 None:intergenic 30.0%
! TGTGAATTTTCTTAGATCTG+AGG + chr4_2:51583274-51583293 Msa0605640:intron 30.0%
!!! ATCTGAGGTTATTTTTGTTC+AGG + chr4_2:51583289-51583308 Msa0605640:intron 30.0%
TTGGTTGAAGTTTAGTGATT+TGG + chr4_2:51583834-51583853 Msa0605640:three_prime_UTR 30.0%
GTTTATACAAAACCAGATTC+AGG - chr4_2:51583906-51583925 None:intergenic 30.0%
ATTAGGGGAAAAAAAGATGA+AGG - chr4_2:51582925-51582944 None:intergenic 30.0%
! TGTGAATTTTCTTAGATCTG+AGG + chr4_2:51583274-51583293 Msa0605640:intron 30.0%
!!! ATCTGAGGTTATTTTTGTTC+AGG + chr4_2:51583289-51583308 Msa0605640:intron 30.0%
TTGGTTGAAGTTTAGTGATT+TGG + chr4_2:51583834-51583853 Msa0605640:three_prime_UTR 30.0%
GTTTATACAAAACCAGATTC+AGG - chr4_2:51583906-51583925 None:intergenic 30.0%
! TTTTTCCCCTAATAGTTCGA+TGG + chr4_2:51582932-51582951 Msa0605640:exon 35.0%
! TTTTCCCCTAATAGTTCGAT+GGG + chr4_2:51582933-51582952 Msa0605640:exon 35.0%
AAGAACCCATCGAACTATTA+GGG - chr4_2:51582941-51582960 None:intergenic 35.0%
GATTTCACCAAACTTAGAGA+AGG - chr4_2:51583033-51583052 None:intergenic 35.0%
! CTTTTGTTACTCACACCAAT+AGG + chr4_2:51583170-51583189 Msa0605640:intron 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_2:51583188-51583207 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_2:51583214-51583233 Msa0605640:intron 35.0%
! AAGATCGATCGGTTTCTATT+CGG + chr4_2:51583225-51583244 Msa0605640:intron 35.0%
GTTATCAATGATCGTGAAAC+TGG + chr4_2:51583311-51583330 Msa0605640:CDS 35.0%
GATGCAATTGAAGAGATGAA+CGG + chr4_2:51583386-51583405 Msa0605640:CDS 35.0%
AGAAAGTTAGGTCTTCAGTT+TGG + chr4_2:51583765-51583784 Msa0605640:exon 35.0%
!!! TGTCTTTTTTGTCCTGAATC+TGG + chr4_2:51583891-51583910 Msa0605640:three_prime_UTR 35.0%
! TTTTTCCCCTAATAGTTCGA+TGG + chr4_2:51582932-51582951 Msa0605640:exon 35.0%
! TTTTCCCCTAATAGTTCGAT+GGG + chr4_2:51582933-51582952 Msa0605640:exon 35.0%
AAGAACCCATCGAACTATTA+GGG - chr4_2:51582941-51582960 None:intergenic 35.0%
GATTTCACCAAACTTAGAGA+AGG - chr4_2:51583033-51583052 None:intergenic 35.0%
! CTTTTGTTACTCACACCAAT+AGG + chr4_2:51583170-51583189 Msa0605640:intron 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_2:51583188-51583207 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_2:51583214-51583233 Msa0605640:intron 35.0%
! AAGATCGATCGGTTTCTATT+CGG + chr4_2:51583225-51583244 Msa0605640:intron 35.0%
GTTATCAATGATCGTGAAAC+TGG + chr4_2:51583311-51583330 Msa0605640:CDS 35.0%
GATGCAATTGAAGAGATGAA+CGG + chr4_2:51583386-51583405 Msa0605640:CDS 35.0%
AGAAAGTTAGGTCTTCAGTT+TGG + chr4_2:51583765-51583784 Msa0605640:exon 35.0%
!!! TGTCTTTTTTGTCCTGAATC+TGG + chr4_2:51583891-51583910 Msa0605640:three_prime_UTR 35.0%
AGAACCCATCGAACTATTAG+GGG - chr4_2:51582940-51582959 None:intergenic 40.0%
GAAGAACCCATCGAACTATT+AGG - chr4_2:51582942-51582961 None:intergenic 40.0%
GTTCTTCTGATGTTGAGTAC+CGG + chr4_2:51582955-51582974 Msa0605640:CDS 40.0%
!! TGGTGAAATCACTGATTCCA+AGG + chr4_2:51583043-51583062 Msa0605640:CDS 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_2:51583218-51583237 None:intergenic 40.0%
!! GATCGGTTTCTATTCGGTTT+TGG + chr4_2:51583231-51583250 Msa0605640:intron 40.0%
! ACTGGAAGATCAAGAGGTTT+TGG + chr4_2:51583329-51583348 Msa0605640:CDS 40.0%
AATTGAAGAGATGAACGGTC+AGG + chr4_2:51583391-51583410 Msa0605640:CDS 40.0%
ATGAACGGTCAGGATATTGA+CGG + chr4_2:51583401-51583420 Msa0605640:CDS 40.0%
ACTGGAGGGATTAGAAAGTT+AGG + chr4_2:51583753-51583772 Msa0605640:exon 40.0%
AAGTTAGGTCTTCAGTTTGG+TGG + chr4_2:51583768-51583787 Msa0605640:three_prime_UTR 40.0%
! ATTTTGAGCAGTGAACTCAG+AGG - chr4_2:51582897-51582916 None:intergenic 40.0%
AGAACCCATCGAACTATTAG+GGG - chr4_2:51582940-51582959 None:intergenic 40.0%
GAAGAACCCATCGAACTATT+AGG - chr4_2:51582942-51582961 None:intergenic 40.0%
GTTCTTCTGATGTTGAGTAC+CGG + chr4_2:51582955-51582974 Msa0605640:CDS 40.0%
!! TGGTGAAATCACTGATTCCA+AGG + chr4_2:51583043-51583062 Msa0605640:CDS 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_2:51583218-51583237 None:intergenic 40.0%
!! GATCGGTTTCTATTCGGTTT+TGG + chr4_2:51583231-51583250 Msa0605640:intron 40.0%
! ACTGGAAGATCAAGAGGTTT+TGG + chr4_2:51583329-51583348 Msa0605640:CDS 40.0%
AATTGAAGAGATGAACGGTC+AGG + chr4_2:51583391-51583410 Msa0605640:CDS 40.0%
ATGAACGGTCAGGATATTGA+CGG + chr4_2:51583401-51583420 Msa0605640:CDS 40.0%
ACTGGAGGGATTAGAAAGTT+AGG + chr4_2:51583753-51583772 Msa0605640:exon 40.0%
AAGTTAGGTCTTCAGTTTGG+TGG + chr4_2:51583768-51583787 Msa0605640:three_prime_UTR 40.0%
GAGCAAGCCTTCTCTAAGTT+TGG + chr4_2:51583023-51583042 Msa0605640:CDS 45.0%
AATCCGAATCAGAATCCGCA+CGG - chr4_2:51583070-51583089 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_2:51583323-51583342 Msa0605640:CDS 45.0%
! TGGTTATGGTGGAAGTGGTA+GGG + chr4_2:51583637-51583656 Msa0605640:CDS 45.0%
GAGCAAGCCTTCTCTAAGTT+TGG + chr4_2:51583023-51583042 Msa0605640:CDS 45.0%
AATCCGAATCAGAATCCGCA+CGG - chr4_2:51583070-51583089 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_2:51583323-51583342 Msa0605640:CDS 45.0%
! TGGTTATGGTGGAAGTGGTA+GGG + chr4_2:51583637-51583656 Msa0605640:CDS 45.0%
GTGAATGAAGCTCAGTCACG+TGG + chr4_2:51583437-51583456 Msa0605640:CDS 50.0%
!! GATGGTGGATACAGCAGAAG+TGG + chr4_2:51583560-51583579 Msa0605640:CDS 50.0%
! GATCGTGGATATGGTGGAGT+TGG + chr4_2:51583611-51583630 Msa0605640:CDS 50.0%
! GTGGTTATGGTGGAAGTGGT+AGG + chr4_2:51583636-51583655 Msa0605640:CDS 50.0%
!! TGATAGCGGTGGAAACTGGA+GGG + chr4_2:51583739-51583758 Msa0605640:CDS 50.0%
GTGAATGAAGCTCAGTCACG+TGG + chr4_2:51583437-51583456 Msa0605640:CDS 50.0%
!! GATGGTGGATACAGCAGAAG+TGG + chr4_2:51583560-51583579 Msa0605640:CDS 50.0%
! GATCGTGGATATGGTGGAGT+TGG + chr4_2:51583611-51583630 Msa0605640:CDS 50.0%
! GTGGTTATGGTGGAAGTGGT+AGG + chr4_2:51583636-51583655 Msa0605640:CDS 50.0%
!! TGATAGCGGTGGAAACTGGA+GGG + chr4_2:51583739-51583758 Msa0605640:CDS 50.0%
!! GTTGAGTACCGGTGCTTCGT+CGG + chr4_2:51582966-51582985 Msa0605640:CDS 55.0%
! CTTCGTCGGTGGTCTTGCTT+GGG + chr4_2:51582980-51582999 Msa0605640:CDS 55.0%
CTCCAGAGCTTGGCTATCAG+TGG - chr4_2:51583006-51583025 None:intergenic 55.0%
! AGAAGGCTTGCTCCAGAGCT+TGG - chr4_2:51583016-51583035 None:intergenic 55.0%
!! CACTGATTCCAAGGTCCGTG+CGG + chr4_2:51583052-51583071 Msa0605640:intron 55.0%
ATCAGAATCCGCACGGACCT+TGG - chr4_2:51583063-51583082 None:intergenic 55.0%
GGTCCGTGCGGATTCTGATT+CGG + chr4_2:51583064-51583083 Msa0605640:intron 55.0%
ACGGTCAGGATATTGACGGC+AGG + chr4_2:51583405-51583424 Msa0605640:CDS 55.0%
!! TATGGTGGCGGTGGTGGTTA+TGG + chr4_2:51583497-51583516 Msa0605640:CDS 55.0%
GGTGGATACAGCAGAAGTGG+TGG + chr4_2:51583563-51583582 Msa0605640:CDS 55.0%
GGATACAGCAGAAGTGGTGG+TGG + chr4_2:51583566-51583585 Msa0605640:CDS 55.0%
!! TACAGCAGAAGTGGTGGTGG+TGG + chr4_2:51583569-51583588 Msa0605640:CDS 55.0%
!! AGTGGTGGTGGTGGAGGATA+TGG + chr4_2:51583578-51583597 Msa0605640:CDS 55.0%
! TATGGTGGAGGTGGTGATCG+TGG + chr4_2:51583596-51583615 Msa0605640:CDS 55.0%
GGAGGTGGTGATCGTGGATA+TGG + chr4_2:51583602-51583621 Msa0605640:CDS 55.0%
GGTGGTGATCGTGGATATGG+TGG + chr4_2:51583605-51583624 Msa0605640:CDS 55.0%
! CGTGGATATGGTGGAGTTGG+TGG + chr4_2:51583614-51583633 Msa0605640:CDS 55.0%
!! GGATATGGTGGAGTTGGTGG+TGG + chr4_2:51583617-51583636 Msa0605640:CDS 55.0%
!! GGTGGAGTTGGTGGTGGTTA+TGG + chr4_2:51583623-51583642 Msa0605640:CDS 55.0%
!! GGAGTTGGTGGTGGTTATGG+TGG + chr4_2:51583626-51583645 Msa0605640:CDS 55.0%
! GGTGGTGGTTATGGTGGAAG+TGG + chr4_2:51583632-51583651 Msa0605640:CDS 55.0%
AGTGGTAGGGACCGTGGATA+TGG + chr4_2:51583650-51583669 Msa0605640:CDS 55.0%
CACCTCCACCACCATATCCA+CGG - chr4_2:51583664-51583683 None:intergenic 55.0%
!! GTGCTGATAGCGGTGGAAAC+TGG + chr4_2:51583735-51583754 Msa0605640:CDS 55.0%
!! CTGATAGCGGTGGAAACTGG+AGG + chr4_2:51583738-51583757 Msa0605640:CDS 55.0%
!! GTTGAGTACCGGTGCTTCGT+CGG + chr4_2:51582966-51582985 Msa0605640:CDS 55.0%
! CTTCGTCGGTGGTCTTGCTT+GGG + chr4_2:51582980-51582999 Msa0605640:CDS 55.0%
CTCCAGAGCTTGGCTATCAG+TGG - chr4_2:51583006-51583025 None:intergenic 55.0%
! AGAAGGCTTGCTCCAGAGCT+TGG - chr4_2:51583016-51583035 None:intergenic 55.0%
!! CACTGATTCCAAGGTCCGTG+CGG + chr4_2:51583052-51583071 Msa0605640:intron 55.0%
ATCAGAATCCGCACGGACCT+TGG - chr4_2:51583063-51583082 None:intergenic 55.0%
GGTCCGTGCGGATTCTGATT+CGG + chr4_2:51583064-51583083 Msa0605640:intron 55.0%
ACGGTCAGGATATTGACGGC+AGG + chr4_2:51583405-51583424 Msa0605640:CDS 55.0%
!! TATGGTGGCGGTGGTGGTTA+TGG + chr4_2:51583497-51583516 Msa0605640:CDS 55.0%
GGTGGATACAGCAGAAGTGG+TGG + chr4_2:51583563-51583582 Msa0605640:CDS 55.0%
GGATACAGCAGAAGTGGTGG+TGG + chr4_2:51583566-51583585 Msa0605640:CDS 55.0%
!! TACAGCAGAAGTGGTGGTGG+TGG + chr4_2:51583569-51583588 Msa0605640:CDS 55.0%
!! AGTGGTGGTGGTGGAGGATA+TGG + chr4_2:51583578-51583597 Msa0605640:CDS 55.0%
! TATGGTGGAGGTGGTGATCG+TGG + chr4_2:51583596-51583615 Msa0605640:CDS 55.0%
GGAGGTGGTGATCGTGGATA+TGG + chr4_2:51583602-51583621 Msa0605640:CDS 55.0%
GGTGGTGATCGTGGATATGG+TGG + chr4_2:51583605-51583624 Msa0605640:CDS 55.0%
! CGTGGATATGGTGGAGTTGG+TGG + chr4_2:51583614-51583633 Msa0605640:CDS 55.0%
!! GGATATGGTGGAGTTGGTGG+TGG + chr4_2:51583617-51583636 Msa0605640:CDS 55.0%
!! GGTGGAGTTGGTGGTGGTTA+TGG + chr4_2:51583623-51583642 Msa0605640:CDS 55.0%
!! GGAGTTGGTGGTGGTTATGG+TGG + chr4_2:51583626-51583645 Msa0605640:CDS 55.0%
! GGTGGTGGTTATGGTGGAAG+TGG + chr4_2:51583632-51583651 Msa0605640:CDS 55.0%
AGTGGTAGGGACCGTGGATA+TGG + chr4_2:51583650-51583669 Msa0605640:CDS 55.0%
CACCTCCACCACCATATCCA+CGG - chr4_2:51583664-51583683 None:intergenic 55.0%
!! GTGCTGATAGCGGTGGAAAC+TGG + chr4_2:51583735-51583754 Msa0605640:CDS 55.0%
!! CTGATAGCGGTGGAAACTGG+AGG + chr4_2:51583738-51583757 Msa0605640:CDS 55.0%
CAAGACCACCGACGAAGCAC+CGG - chr4_2:51582977-51582996 None:intergenic 60.0%
! GCTTCGTCGGTGGTCTTGCT+TGG + chr4_2:51582979-51582998 Msa0605640:CDS 60.0%
GGCCACTGATAGCCAAGCTC+TGG + chr4_2:51583001-51583020 Msa0605640:CDS 60.0%
GAAGCTCAGTCACGTGGCAG+TGG + chr4_2:51583443-51583462 Msa0605640:CDS 60.0%
! AGTGGTGGAGGAGGTAGAGG+TGG + chr4_2:51583461-51583480 Msa0605640:CDS 60.0%
! GGTGGTGGTTATGGAGGTGG+CGG + chr4_2:51583506-51583525 Msa0605640:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + chr4_2:51583527-51583546 Msa0605640:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_2:51583530-51583549 Msa0605640:CDS 60.0%
!! AGCAGAAGTGGTGGTGGTGG+AGG + chr4_2:51583572-51583591 Msa0605640:CDS 60.0%
!! GGTGGTGGTGGAGGATATGG+TGG + chr4_2:51583581-51583600 Msa0605640:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+AGG + chr4_2:51583584-51583603 Msa0605640:CDS 60.0%
! GGTGGAGGATATGGTGGAGG+TGG + chr4_2:51583587-51583606 Msa0605640:CDS 60.0%
GGTAGGGACCGTGGATATGG+TGG + chr4_2:51583653-51583672 Msa0605640:CDS 60.0%
AGGGACCGTGGATATGGTGG+TGG + chr4_2:51583656-51583675 Msa0605640:CDS 60.0%
!! GACCGTGGATATGGTGGTGG+AGG + chr4_2:51583659-51583678 Msa0605640:CDS 60.0%
!! CGTGGATATGGTGGTGGAGG+TGG + chr4_2:51583662-51583681 Msa0605640:CDS 60.0%
GGAGGTGGTGACCGTGGTTA+TGG + chr4_2:51583677-51583696 Msa0605640:CDS 60.0%
GGTGGTGACCGTGGTTATGG+TGG + chr4_2:51583680-51583699 Msa0605640:CDS 60.0%
GGTGACCGTGGTTATGGTGG+TGG + chr4_2:51583683-51583702 Msa0605640:CDS 60.0%
!! GACCGTGGTTATGGTGGTGG+TGG + chr4_2:51583686-51583705 Msa0605640:CDS 60.0%
CGCCACCACCACCATAACCA+CGG - chr4_2:51583691-51583710 None:intergenic 60.0%
!! CGTGGTTATGGTGGTGGTGG+CGG + chr4_2:51583689-51583708 Msa0605640:CDS 60.0%
CAAGACCACCGACGAAGCAC+CGG - chr4_2:51582977-51582996 None:intergenic 60.0%
! GCTTCGTCGGTGGTCTTGCT+TGG + chr4_2:51582979-51582998 Msa0605640:CDS 60.0%
GGCCACTGATAGCCAAGCTC+TGG + chr4_2:51583001-51583020 Msa0605640:CDS 60.0%
GAAGCTCAGTCACGTGGCAG+TGG + chr4_2:51583443-51583462 Msa0605640:CDS 60.0%
! AGTGGTGGAGGAGGTAGAGG+TGG + chr4_2:51583461-51583480 Msa0605640:CDS 60.0%
! GGTGGTGGTTATGGAGGTGG+CGG + chr4_2:51583506-51583525 Msa0605640:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + chr4_2:51583527-51583546 Msa0605640:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_2:51583530-51583549 Msa0605640:CDS 60.0%
!! AGCAGAAGTGGTGGTGGTGG+AGG + chr4_2:51583572-51583591 Msa0605640:CDS 60.0%
!! GGTGGTGGTGGAGGATATGG+TGG + chr4_2:51583581-51583600 Msa0605640:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+AGG + chr4_2:51583584-51583603 Msa0605640:CDS 60.0%
! GGTGGAGGATATGGTGGAGG+TGG + chr4_2:51583587-51583606 Msa0605640:CDS 60.0%
GGTAGGGACCGTGGATATGG+TGG + chr4_2:51583653-51583672 Msa0605640:CDS 60.0%
AGGGACCGTGGATATGGTGG+TGG + chr4_2:51583656-51583675 Msa0605640:CDS 60.0%
!! GACCGTGGATATGGTGGTGG+AGG + chr4_2:51583659-51583678 Msa0605640:CDS 60.0%
!! CGTGGATATGGTGGTGGAGG+TGG + chr4_2:51583662-51583681 Msa0605640:CDS 60.0%
GGAGGTGGTGACCGTGGTTA+TGG + chr4_2:51583677-51583696 Msa0605640:CDS 60.0%
GGTGGTGACCGTGGTTATGG+TGG + chr4_2:51583680-51583699 Msa0605640:CDS 60.0%
GGTGACCGTGGTTATGGTGG+TGG + chr4_2:51583683-51583702 Msa0605640:CDS 60.0%
!! GACCGTGGTTATGGTGGTGG+TGG + chr4_2:51583686-51583705 Msa0605640:CDS 60.0%
CGCCACCACCACCATAACCA+CGG - chr4_2:51583691-51583710 None:intergenic 60.0%
!! CGTGGTTATGGTGGTGGTGG+CGG + chr4_2:51583689-51583708 Msa0605640:CDS 60.0%
!! GAGTACCGGTGCTTCGTCGG+TGG + chr4_2:51582969-51582988 Msa0605640:CDS 65.0%
GCTCAGTCACGTGGCAGTGG+TGG + chr4_2:51583446-51583465 Msa0605640:CDS 65.0%
CAGTCACGTGGCAGTGGTGG+AGG + chr4_2:51583449-51583468 Msa0605640:CDS 65.0%
! TCACGTGGCAGTGGTGGAGG+AGG + chr4_2:51583452-51583471 Msa0605640:CDS 65.0%
! GGCAGTGGTGGAGGAGGTAG+AGG + chr4_2:51583458-51583477 Msa0605640:CDS 65.0%
GGTGGAGGAGGTAGAGGTGG+CGG + chr4_2:51583464-51583483 Msa0605640:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+CGG + chr4_2:51583485-51583504 Msa0605640:CDS 65.0%
!! GGTGGTGGATATGGTGGCGG+TGG + chr4_2:51583488-51583507 Msa0605640:CDS 65.0%
!! GGTGGATATGGTGGCGGTGG+TGG + chr4_2:51583491-51583510 Msa0605640:CDS 65.0%
! GGTGGCGGTGGTGGTTATGG+AGG + chr4_2:51583500-51583519 Msa0605640:CDS 65.0%
! GGCGGTGGTGGTTATGGAGG+TGG + chr4_2:51583503-51583522 Msa0605640:CDS 65.0%
! GGTGGTTATGGAGGTGGCGG+TGG + chr4_2:51583509-51583528 Msa0605640:CDS 65.0%
! GGTTATGGAGGTGGCGGTGG+TGG + chr4_2:51583512-51583531 Msa0605640:CDS 65.0%
!! GGAGGTGGCGGTGGTGGATA+TGG + chr4_2:51583518-51583537 Msa0605640:CDS 65.0%
!! GGTGGCGGTGGTGGATATGG+TGG + chr4_2:51583521-51583540 Msa0605640:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+AGG + chr4_2:51583485-51583504 Msa0605640:CDS 65.0%
! GGAGGTGGTGGTCGTCGTGA+TGG + chr4_2:51583542-51583561 Msa0605640:CDS 65.0%
! GGTGGTGGTCGTCGTGATGG+TGG + chr4_2:51583545-51583564 Msa0605640:CDS 65.0%
GGTGGAAGTGGTAGGGACCG+TGG + chr4_2:51583644-51583663 Msa0605640:CDS 65.0%
GGCGGTGATCGCTACTCTCG+CGG + chr4_2:51583707-51583726 Msa0605640:CDS 65.0%
GGTGATCGCTACTCTCGCGG+TGG + chr4_2:51583710-51583729 Msa0605640:CDS 65.0%
GATCGCTACTCTCGCGGTGG+TGG + chr4_2:51583713-51583732 Msa0605640:CDS 65.0%
!! CGCGGTGGTGGTGCTGATAG+CGG + chr4_2:51583725-51583744 Msa0605640:CDS 65.0%
!! GGTGGTGGTGCTGATAGCGG+TGG + chr4_2:51583728-51583747 Msa0605640:CDS 65.0%
!! GAGTACCGGTGCTTCGTCGG+TGG + chr4_2:51582969-51582988 Msa0605640:CDS 65.0%
GCTCAGTCACGTGGCAGTGG+TGG + chr4_2:51583446-51583465 Msa0605640:CDS 65.0%
CAGTCACGTGGCAGTGGTGG+AGG + chr4_2:51583449-51583468 Msa0605640:CDS 65.0%
! TCACGTGGCAGTGGTGGAGG+AGG + chr4_2:51583452-51583471 Msa0605640:CDS 65.0%
! GGCAGTGGTGGAGGAGGTAG+AGG + chr4_2:51583458-51583477 Msa0605640:CDS 65.0%
GGTGGAGGAGGTAGAGGTGG+CGG + chr4_2:51583464-51583483 Msa0605640:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+CGG + chr4_2:51583485-51583504 Msa0605640:CDS 65.0%
!! GGTGGTGGATATGGTGGCGG+TGG + chr4_2:51583488-51583507 Msa0605640:CDS 65.0%
!! GGTGGATATGGTGGCGGTGG+TGG + chr4_2:51583491-51583510 Msa0605640:CDS 65.0%
! GGTGGCGGTGGTGGTTATGG+AGG + chr4_2:51583500-51583519 Msa0605640:CDS 65.0%
! GGCGGTGGTGGTTATGGAGG+TGG + chr4_2:51583503-51583522 Msa0605640:CDS 65.0%
! GGTGGTTATGGAGGTGGCGG+TGG + chr4_2:51583509-51583528 Msa0605640:CDS 65.0%
! GGTTATGGAGGTGGCGGTGG+TGG + chr4_2:51583512-51583531 Msa0605640:CDS 65.0%
!! GGAGGTGGCGGTGGTGGATA+TGG + chr4_2:51583518-51583537 Msa0605640:CDS 65.0%
!! GGTGGCGGTGGTGGATATGG+TGG + chr4_2:51583521-51583540 Msa0605640:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+AGG + chr4_2:51583485-51583504 Msa0605640:CDS 65.0%
! GGAGGTGGTGGTCGTCGTGA+TGG + chr4_2:51583542-51583561 Msa0605640:CDS 65.0%
! GGTGGTGGTCGTCGTGATGG+TGG + chr4_2:51583545-51583564 Msa0605640:CDS 65.0%
GGTGGAAGTGGTAGGGACCG+TGG + chr4_2:51583644-51583663 Msa0605640:CDS 65.0%
GGCGGTGATCGCTACTCTCG+CGG + chr4_2:51583707-51583726 Msa0605640:CDS 65.0%
GGTGATCGCTACTCTCGCGG+TGG + chr4_2:51583710-51583729 Msa0605640:CDS 65.0%
GATCGCTACTCTCGCGGTGG+TGG + chr4_2:51583713-51583732 Msa0605640:CDS 65.0%
!! CGCGGTGGTGGTGCTGATAG+CGG + chr4_2:51583725-51583744 Msa0605640:CDS 65.0%
!! GGTGGTGGTGCTGATAGCGG+TGG + chr4_2:51583728-51583747 Msa0605640:CDS 65.0%
! GGAGGAGGTAGAGGTGGCGG+CGG + chr4_2:51583467-51583486 Msa0605640:CDS 70.0%
!! GGTGGCGGCGGTGGTGGATA+TGG + chr4_2:51583479-51583498 Msa0605640:CDS 70.0%
!! GGCGGCGGTGGTGGATATGG+TGG + chr4_2:51583482-51583501 Msa0605640:CDS 70.0%
!! GGTGGTGGAGGTGGTGACCG+TGG + chr4_2:51583671-51583690 Msa0605640:CDS 70.0%
! GGAGGAGGTAGAGGTGGCGG+CGG + chr4_2:51583467-51583486 Msa0605640:CDS 70.0%
!! GGTGGCGGCGGTGGTGGATA+TGG + chr4_2:51583479-51583498 Msa0605640:CDS 70.0%
!! GGCGGCGGTGGTGGATATGG+TGG + chr4_2:51583482-51583501 Msa0605640:CDS 70.0%
!! GGTGGTGGAGGTGGTGACCG+TGG + chr4_2:51583671-51583690 Msa0605640:CDS 70.0%
! GGAGGTAGAGGTGGCGGCGG+TGG + chr4_2:51583470-51583489 Msa0605640:CDS 75.0%
! GGTAGAGGTGGCGGCGGTGG+TGG + chr4_2:51583473-51583492 Msa0605640:CDS 75.0%
! GGAGGTAGAGGTGGCGGCGG+TGG + chr4_2:51583470-51583489 Msa0605640:CDS 75.0%
! GGTAGAGGTGGCGGCGGTGG+TGG + chr4_2:51583473-51583492 Msa0605640:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51582896 51583975 51582896 ID=Msa0605640;Name=Msa0605640
chr4_2 mRNA 51582896 51583975 51582896 ID=Msa0605640-mRNA-1;Parent=Msa0605640;Name=Msa0605640-mRNA-1;_AED=0.29;_eAED=0.29;_QI=55|1|1|1|1|1|2|209|189
chr4_2 exon 51582896 51583064 51582896 ID=Msa0605640-mRNA-1:exon:12358;Parent=Msa0605640-mRNA-1
chr4_2 exon 51583311 51583975 51583311 ID=Msa0605640-mRNA-1:exon:12359;Parent=Msa0605640-mRNA-1
chr4_2 five_prime_UTR 51582896 51582950 51582896 ID=Msa0605640-mRNA-1:five_prime_utr;Parent=Msa0605640-mRNA-1
chr4_2 CDS 51582951 51583064 51582951 ID=Msa0605640-mRNA-1:cds;Parent=Msa0605640-mRNA-1
chr4_2 CDS 51583311 51583766 51583311 ID=Msa0605640-mRNA-1:cds;Parent=Msa0605640-mRNA-1
chr4_2 three_prime_UTR 51583767 51583975 51583767 ID=Msa0605640-mRNA-1:three_prime_utr;Parent=Msa0605640-mRNA-1
Gene Sequence

>Msa0605640

ATGGGTTCTTCTGATGTTGAGTACCGGTGCTTCGTCGGTGGTCTTGCTTGGGCCACTGATAGCCAAGCTCTGGAGCAAGCCTTCTCTAAGTTTGGTGAAATCACTGATTCCAAGGTTATCAATGATCGTGAAACTGGAAGATCAAGAGGTTTTGGATTTGTGACATTTGCTGATGAGAAATCGATGAGAGATGCAATTGAAGAGATGAACGGTCAGGATATTGACGGCAGGAATATTACTGTGAATGAAGCTCAGTCACGTGGCAGTGGTGGAGGAGGTAGAGGTGGCGGCGGTGGTGGATATGGTGGCGGTGGTGGTTATGGAGGTGGCGGTGGTGGATATGGTGGAGGTGGTGGTCGTCGTGATGGTGGATACAGCAGAAGTGGTGGTGGTGGAGGATATGGTGGAGGTGGTGATCGTGGATATGGTGGAGTTGGTGGTGGTTATGGTGGAAGTGGTAGGGACCGTGGATATGGTGGTGGAGGTGGTGACCGTGGTTATGGTGGTGGTGGCGGTGATCGCTACTCTCGCGGTGGTGGTGCTGATAGCGGTGGAAACTGGAGGGATTAG
Protein sequence

>Msa0605640

MGSSDVEYRCFVGGLAWATDSQALEQAFSKFGEITDSKVINDRETGRSRGFGFVTFADEKSMRDAIEEMNGQDIDGRNITVNEAQSRGSGGGGRGGGGGGYGGGGGYGGGGGGYGGGGGRRDGGYSRSGGGGGYGGGGDRGYGGVGGGYGGSGRDRGYGGGGGDRGYGGGGGDRYSRGGGADSGGNWRD*