AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0550130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550130 MtrunA17_Chr4g0035031 97.368 190 4 1 1 189 1 190 1.02e-108 308
Msa0550130 MtrunA17_Chr4g0035051 88.235 85 10 0 1 85 1 85 1.26e-52 164
Msa0550130 MtrunA17_Chr4g0035001 73.103 145 30 1 4 148 2 137 3.95e-40 134
Msa0550130 MtrunA17_Chr2g0278661 56.180 89 38 1 1 89 1 88 1.41e-31 114
Msa0550130 MtrunA17_Chr4g0050781 44.516 155 81 2 3 157 2 151 5.97e-25 96.7
Msa0550130 MtrunA17_Chr3g0086861 47.059 85 45 0 3 87 39 123 6.32e-23 94.7
Msa0550130 MtrunA17_Chr3g0116631 51.852 81 39 0 8 88 206 286 7.96e-23 92.4
Msa0550130 MtrunA17_Chr3g0116631 27.815 151 99 1 9 159 110 250 8.74e-12 62.4
Msa0550130 MtrunA17_Chr4g0061451 47.500 80 42 0 9 88 43 122 2.46e-22 90.9
Msa0550130 MtrunA17_Chr7g0219021 44.578 83 46 0 9 91 41 123 6.72e-22 89.4
Msa0550130 MtrunA17_Chr3g0121631 50.633 79 38 1 9 87 37 114 1.09e-20 83.6
Msa0550130 MtrunA17_Chr4g0058151 50.617 81 40 0 8 88 216 296 1.59e-20 86.7
Msa0550130 MtrunA17_Chr4g0058151 40.964 83 49 0 8 90 122 204 3.89e-14 68.9
Msa0550130 MtrunA17_Chr1g0181111 50.575 87 43 0 2 88 190 276 2.09e-18 80.5
Msa0550130 MtrunA17_Chr1g0181111 36.283 113 71 1 9 120 85 197 1.99e-11 61.2
Msa0550130 MtrunA17_Chr3g0119311 40.244 82 49 0 9 90 37 118 2.21e-18 77.4
Msa0550130 MtrunA17_Chr7g0266751 40.741 81 48 0 6 86 30 110 3.62e-17 74.3
Msa0550130 MtrunA17_Chr3g0144311 46.053 76 40 1 9 84 30 104 1.93e-16 75.1
Msa0550130 MtrunA17_Chr4g0017401 41.000 100 52 4 5 99 12 109 2.81e-16 74.7
Msa0550130 MtrunA17_Chr1g0168601 41.463 82 48 0 4 85 149 230 1.77e-15 72.0
Msa0550130 MtrunA17_Chr5g0447761 37.975 79 47 1 9 87 7 83 2.13e-15 73.6
Msa0550130 MtrunA17_Chr5g0447761 32.258 93 62 1 9 101 107 198 3.47e-11 61.2
Msa0550130 MtrunA17_Chr3g0101061 37.975 79 47 1 9 87 41 117 3.13e-15 72.8
Msa0550130 MtrunA17_Chr3g0101061 32.000 150 88 4 9 145 141 289 3.11e-11 61.2
Msa0550130 MtrunA17_Chr7g0276531 46.053 76 40 1 9 84 19 93 4.64e-15 71.2
Msa0550130 MtrunA17_Chr2g0327921 39.024 82 50 0 9 90 8 89 7.58e-15 66.6
Msa0550130 MtrunA17_Chr3g0127751 45.000 80 43 1 9 88 27 105 8.35e-15 70.5
Msa0550130 MtrunA17_Chr5g0426311 44.737 76 41 1 9 84 17 91 8.92e-15 70.9
Msa0550130 MtrunA17_Chr2g0290191 39.241 79 46 1 9 87 5 81 9.42e-15 71.6
Msa0550130 MtrunA17_Chr2g0290191 33.835 133 78 3 9 131 107 239 1.55e-12 65.1
Msa0550130 MtrunA17_Chr1g0186351 35.443 79 49 1 9 87 7 83 1.84e-14 70.9
Msa0550130 MtrunA17_Chr1g0186351 34.118 85 55 1 9 93 113 196 1.11e-12 65.5
Msa0550130 MtrunA17_Chr8g0346011 32.639 144 86 5 9 145 38 177 2.17e-14 69.7
Msa0550130 MtrunA17_Chr4g0065741 37.500 96 57 1 1 96 67 159 3.86e-14 67.0
Msa0550130 MtrunA17_Chr6g0449481 38.095 84 50 1 9 92 46 127 1.14e-13 68.2
Msa0550130 MtrunA17_Chr6g0449481 33.858 127 80 1 9 131 134 260 1.79e-11 62.0
Msa0550130 MtrunA17_Chr5g0406171 32.386 176 99 5 10 171 133 302 1.15e-13 67.8
Msa0550130 MtrunA17_Chr3g0120721 32.374 139 86 1 9 147 107 237 1.21e-13 68.2
Msa0550130 MtrunA17_Chr3g0120721 36.585 82 50 1 9 90 19 98 2.03e-13 67.4
Msa0550130 MtrunA17_Chr7g0263001 37.931 87 54 0 1 87 28 114 2.67e-13 64.3
Msa0550130 MtrunA17_Chr7g0219031 42.424 66 38 0 9 74 41 106 3.19e-13 63.2
Msa0550130 MtrunA17_Chr6g0481071 38.750 80 49 0 9 88 96 175 8.77e-13 63.5
Msa0550130 MtrunA17_Chr4g0042161 38.298 94 52 2 11 101 54 144 1.80e-12 62.0
Msa0550130 MtrunA17_Chr5g0402191 41.250 80 43 2 9 87 5 81 2.68e-12 64.3
Msa0550130 MtrunA17_Chr4g0031091 39.726 73 44 0 9 81 34 106 8.57e-12 59.3
Msa0550130 MtrunA17_Chr4g0018581 44.444 72 40 0 10 81 453 524 9.28e-12 63.2
Msa0550130 MtrunA17_Chr1g0198431 35.870 92 56 1 9 97 193 284 1.11e-11 62.0
Msa0550130 MtrunA17_Chr3g0110211 39.175 97 58 1 3 98 107 203 1.30e-11 62.4
Msa0550130 MtrunA17_Chr3g0135111 31.532 111 75 1 9 119 118 227 1.94e-11 61.6
Msa0550130 MtrunA17_Chr4g0062931 34.503 171 91 7 10 179 9 159 2.68e-11 61.6
Msa0550130 MtrunA17_Chr5g0419351 36.957 92 58 0 10 101 12 103 3.39e-11 59.3
Msa0550130 MtrunA17_Chr3g0088511 38.356 73 45 0 9 81 33 105 6.92e-11 57.4
Msa0550130 MtrunA17_Chr8g0360551 35.955 89 55 2 9 97 74 160 7.81e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550130 AT4G39260.3 77.778 90 20 0 4 93 2 91 4.84e-48 152
Msa0550130 AT2G21660.2 78.824 85 18 0 1 85 1 85 3.62e-45 147
Msa0550130 AT2G21660.1 78.824 85 18 0 1 85 1 85 6.04e-45 147
Msa0550130 AT4G39260.1 75.610 82 20 0 4 85 2 83 2.51e-42 140
Msa0550130 AT4G39260.2 71.171 111 29 1 4 114 2 109 1.34e-38 129
Msa0550130 AT4G39260.4 76.119 67 16 0 4 70 2 68 3.40e-34 117
Msa0550130 AT3G26420.1 54.167 96 43 1 3 98 2 96 4.00e-31 113
Msa0550130 AT4G13850.2 54.217 83 38 0 9 91 36 118 3.23e-25 95.9
Msa0550130 AT3G23830.2 52.475 101 43 1 9 109 36 131 6.69e-24 92.0
Msa0550130 AT3G23830.1 52.475 101 43 1 9 109 36 131 6.69e-24 92.0
Msa0550130 AT5G04280.1 47.475 99 47 1 9 102 8 106 9.47e-24 95.9
Msa0550130 AT4G13850.3 56.566 99 43 0 9 107 36 134 1.23e-23 91.7
Msa0550130 AT4G13850.1 57.426 101 43 0 9 109 36 136 2.38e-23 91.3
Msa0550130 AT4G13850.4 56.383 94 40 1 9 101 36 129 3.48e-23 90.1
Msa0550130 AT5G61030.2 49.367 79 40 0 9 87 41 119 2.14e-22 92.0
Msa0550130 AT5G61030.1 49.367 79 40 0 9 87 41 119 2.14e-22 92.0
Msa0550130 AT2G21690.1 50.000 90 40 1 3 87 2 91 2.50e-21 84.7
Msa0550130 AT4G24770.1 47.561 82 43 0 8 89 244 325 1.62e-20 87.4
Msa0550130 AT4G24770.1 45.783 83 45 0 7 89 149 231 9.74e-16 74.3
Msa0550130 AT4G24770.2 47.561 82 43 0 8 89 244 325 1.62e-20 87.4
Msa0550130 AT4G24770.2 45.783 83 45 0 7 89 149 231 9.74e-16 74.3
Msa0550130 AT5G06210.1 44.318 88 49 0 6 93 32 119 1.89e-20 83.6
Msa0550130 AT5G50250.1 48.193 83 43 0 5 87 204 286 1.00e-19 84.7
Msa0550130 AT5G50250.1 32.680 153 90 2 7 159 112 251 4.91e-16 74.7
Msa0550130 AT3G53460.1 45.783 83 45 0 9 91 258 340 1.94e-19 84.7
Msa0550130 AT3G53460.3 45.783 83 45 0 9 91 258 340 1.94e-19 84.7
Msa0550130 AT3G53460.2 45.783 83 45 0 9 91 258 340 1.94e-19 84.7
Msa0550130 AT2G37220.1 45.679 81 44 0 9 89 205 285 2.38e-19 83.6
Msa0550130 AT3G53460.4 45.783 83 45 0 9 91 279 361 2.58e-19 84.3
Msa0550130 AT1G60650.2 42.169 83 48 0 5 87 9 91 4.96e-19 82.8
Msa0550130 AT1G60650.1 42.169 83 48 0 5 87 9 91 4.96e-19 82.8
Msa0550130 AT1G74230.1 52.688 93 39 2 1 93 31 118 5.69e-19 82.4
Msa0550130 AT3G07810.2 41.772 79 44 1 9 87 7 83 2.75e-17 79.3
Msa0550130 AT3G07810.2 33.577 137 73 4 9 131 109 241 3.34e-12 64.7
Msa0550130 AT3G07810.1 41.772 79 44 1 9 87 7 83 2.94e-17 79.3
Msa0550130 AT3G07810.1 33.577 137 73 4 9 131 109 241 3.47e-12 64.7
Msa0550130 AT1G76460.1 48.684 76 38 1 9 84 25 99 5.12e-17 77.4
Msa0550130 AT1G22330.1 44.118 102 51 3 9 105 18 118 8.13e-17 76.6
Msa0550130 AT1G18630.1 46.154 78 42 0 9 86 37 114 1.33e-16 73.6
Msa0550130 AT5G47320.1 46.753 77 41 0 9 85 32 108 2.06e-16 74.3
Msa0550130 AT4G26650.1 34.286 105 67 1 9 113 16 118 2.12e-16 76.6
Msa0550130 AT4G26650.2 34.286 105 67 1 9 113 13 115 2.33e-16 76.6
Msa0550130 AT4G14300.1 40.506 79 45 1 9 87 7 83 3.09e-16 76.3
Msa0550130 AT4G14300.2 40.506 79 45 1 9 87 7 83 3.09e-16 76.3
Msa0550130 AT1G20880.2 46.053 76 40 1 9 84 25 99 5.27e-16 74.3
Msa0550130 AT1G20880.3 46.053 76 40 1 9 84 25 99 5.27e-16 74.3
Msa0550130 AT1G20880.1 46.053 76 40 1 9 84 25 99 5.27e-16 74.3
Msa0550130 AT3G46020.1 42.857 77 44 0 9 85 8 84 1.71e-15 69.3
Msa0550130 AT1G22910.1 40.404 99 54 3 5 99 9 106 1.92e-15 72.4
Msa0550130 AT2G33410.1 39.241 79 46 1 9 87 7 83 2.44e-15 73.6
Msa0550130 AT2G46780.1 46.053 76 40 1 9 84 23 97 2.64e-15 73.2
Msa0550130 AT2G46780.2 46.053 76 40 1 9 84 23 97 2.64e-15 73.2
Msa0550130 AT1G22910.2 40.404 99 54 3 5 99 9 106 2.73e-15 72.0
Msa0550130 AT3G13224.3 38.462 91 50 2 6 92 4 92 4.05e-15 72.8
Msa0550130 AT3G13224.3 32.955 88 58 1 3 89 94 181 8.51e-12 63.2
Msa0550130 AT3G13224.2 39.286 84 49 1 9 92 20 101 5.20e-15 72.4
Msa0550130 AT3G13224.2 32.955 88 58 1 3 89 103 190 2.83e-12 64.7
Msa0550130 AT1G33470.3 43.421 76 42 1 9 84 8 82 5.63e-15 70.9
Msa0550130 AT5G55550.5 33.981 103 66 1 9 111 7 107 5.65e-15 72.4
Msa0550130 AT5G55550.4 33.981 103 66 1 9 111 7 107 5.65e-15 72.4
Msa0550130 AT5G55550.9 33.981 103 66 1 9 111 7 107 5.65e-15 72.4
Msa0550130 AT5G55550.7 33.981 103 66 1 9 111 7 107 5.65e-15 72.4
Msa0550130 AT5G55550.1 33.981 103 66 1 9 111 7 107 5.65e-15 72.4
Msa0550130 AT1G33470.2 43.421 76 42 1 9 84 8 82 6.69e-15 70.9
Msa0550130 AT5G55550.2 33.981 103 66 1 9 111 7 107 6.82e-15 72.4
Msa0550130 AT5G55550.3 33.981 103 66 1 9 111 7 107 6.82e-15 72.4
Msa0550130 AT5G55550.6 33.981 103 66 1 9 111 7 107 6.82e-15 72.4
Msa0550130 AT5G55550.11 33.981 103 66 1 9 111 7 107 6.82e-15 72.4
Msa0550130 AT1G33470.1 43.421 76 42 1 9 84 8 82 7.50e-15 70.9
Msa0550130 AT1G17640.2 42.500 80 44 1 9 88 67 144 1.34e-14 71.2
Msa0550130 AT1G17640.1 42.500 80 44 1 9 88 67 144 1.34e-14 71.2
Msa0550130 AT1G17640.3 42.500 80 44 1 9 88 67 144 1.34e-14 71.2
Msa0550130 AT3G08000.1 43.678 87 43 2 9 92 42 125 1.45e-14 67.8
Msa0550130 AT1G22910.3 40.404 99 54 3 5 99 9 106 1.73e-14 70.9
Msa0550130 AT2G37510.1 45.946 74 40 0 9 82 35 108 1.98e-14 67.4
Msa0550130 AT5G59865.1 44.304 79 44 0 11 89 29 107 3.48e-14 66.2
Msa0550130 AT1G78260.1 43.210 81 44 2 5 84 13 92 5.50e-14 68.9
Msa0550130 AT3G54770.3 43.421 76 42 1 9 84 18 92 5.56e-14 68.6
Msa0550130 AT3G54770.1 43.421 76 42 1 9 84 18 92 7.23e-14 68.2
Msa0550130 AT1G78260.2 43.210 81 44 2 5 84 13 92 8.08e-14 68.2
Msa0550130 AT5G19960.1 36.134 119 64 3 10 127 9 116 8.79e-14 68.9
Msa0550130 AT5G53720.1 42.683 82 46 1 3 84 4 84 2.87e-13 63.5
Msa0550130 AT1G60000.1 40.244 82 49 0 4 85 173 254 4.50e-13 66.2
Msa0550130 AT5G55550.8 40.278 72 41 1 9 80 7 76 5.57e-13 67.0
Msa0550130 AT2G27330.1 29.670 91 63 1 11 100 24 114 5.77e-13 63.2
Msa0550130 AT5G40490.1 38.542 96 57 1 9 104 43 136 5.89e-13 66.6
Msa0550130 AT3G13224.1 39.286 84 49 1 9 92 20 101 6.08e-13 65.5
Msa0550130 AT5G55550.10 40.278 72 41 1 9 80 7 76 6.60e-13 66.6
Msa0550130 AT3G14100.1 42.308 78 45 0 7 84 143 220 6.77e-13 66.6
Msa0550130 AT2G14160.1 39.286 84 32 2 1 66 1 83 8.05e-13 61.6
Msa0550130 AT5G47620.1 36.709 79 48 1 9 87 7 83 9.06e-13 66.2
Msa0550130 AT5G47620.2 36.709 79 48 1 9 87 7 83 9.06e-13 66.2
Msa0550130 AT5G47620.5 36.709 79 48 1 9 87 7 83 9.06e-13 66.2
Msa0550130 AT4G13860.1 40.541 74 44 0 9 82 4 77 1.49e-12 61.2
Msa0550130 AT1G01080.1 41.772 79 41 2 7 83 211 286 2.04e-12 64.7
Msa0550130 AT1G01080.2 41.772 79 41 2 7 83 212 287 2.22e-12 64.3
Msa0550130 AT1G54080.1 42.308 78 45 0 7 84 147 224 2.92e-12 64.7
Msa0550130 AT5G53680.1 46.377 69 36 1 9 77 14 81 6.52e-12 61.6
Msa0550130 AT5G04280.3 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0550130 AT5G04280.4 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0550130 AT5G04280.5 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0550130 AT5G04280.2 47.059 68 31 1 40 102 1 68 1.22e-11 62.4
Msa0550130 AT5G47620.4 37.500 72 43 1 9 80 7 76 1.29e-11 62.8
Msa0550130 AT4G27000.1 43.243 74 41 1 7 79 172 245 2.30e-11 62.0
Msa0550130 AT4G36960.1 36.782 87 53 1 6 90 1 87 3.95e-11 61.2
Msa0550130 AT4G36960.2 36.782 87 53 1 6 90 1 87 3.95e-11 61.2
Msa0550130 AT5G51300.1 41.333 75 44 0 7 81 479 553 4.66e-11 61.2
Msa0550130 AT5G51300.2 41.333 75 44 0 7 81 479 553 4.66e-11 61.2
Msa0550130 AT5G51300.3 41.333 75 44 0 7 81 479 553 4.66e-11 61.2
Msa0550130 AT3G20930.1 35.000 80 52 0 9 88 283 362 6.38e-11 60.8
Msa0550130 AT3G06970.1 30.973 113 71 3 9 120 13 119 9.12e-11 60.1

Find 104 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGTGGAGGTGGTGGTTA+TGG 0.237735 4_1:+45544325 Msa0550130:CDS
ACTGGAAGATCAAGAGGTTT+TGG 0.244628 4_1:+45544157 Msa0550130:CDS
GGTGGAGGTGGTGGTGGTTA+TGG 0.247285 4_1:+45544451 Msa0550130:CDS
GGTAGAGGTGGCGGCGGCGG+TGG 0.247335 4_1:+45544301 Msa0550130:CDS
GGAGGTGGCGGTGGTGGATA+TGG 0.255891 4_1:+45544346 Msa0550130:CDS
AGTGGTGGTGGTGGAGGATA+TGG 0.275624 4_1:+45544406 Msa0550130:CDS
GTATAAACGATTGATTAAAA+AGG 0.284859 4_1:+45544736 Msa0550130:three_prime_UTR
ACTGGAGGGATTAGAAAATT+AGG 0.300294 4_1:+45544581 Msa0550130:exon
TTGGTTGAAGTTTAGTGATT+TGG 0.311002 4_1:+45544652 Msa0550130:three_prime_UTR
GGAGGTGGTGACCGTGGATA+TGG 0.314035 4_1:+45544430 Msa0550130:CDS
GGAGGTGGTGGTGGTTATGG+TGG 0.314652 4_1:+45544454 Msa0550130:CDS
GTTATCAATGATCGTGAAAC+TGG 0.330573 4_1:+45544139 Msa0550130:CDS
GGTGGCGGTGGTGGATATGG+TGG 0.346314 4_1:+45544349 Msa0550130:CDS
GGAGGTGGTGACCGTGGTTA+TGG 0.350450 4_1:+45544505 Msa0550130:CDS
GCTTCGTCGGTGGTCTTGCT+TGG 0.353153 4_1:+45543807 Msa0550130:CDS
GGTGGTTATGGAGGTGGCGG+TGG 0.356362 4_1:+45544337 Msa0550130:CDS
GTTTATACAAAACCAGATTC+AGG 0.358098 4_1:-45544721 None:intergenic
GTGCTGTTAGCGGTGGAAAC+TGG 0.361920 4_1:+45544563 Msa0550130:CDS
GAGCAAGCCTTCTCTAAGTT+TGG 0.374142 4_1:+45543851 Msa0550130:CDS
GGTTATGGAGGTGGCGGTGG+TGG 0.376259 4_1:+45544340 Msa0550130:CDS
GTGGTTATGGTGGAGGTGGT+AGG 0.377057 4_1:+45544464 Msa0550130:CDS
GGAGGTAGAGGTGGCGGCGG+CGG 0.380285 4_1:+45544298 Msa0550130:CDS
CTTCGTCGGTGGTCTTGCTT+GGG 0.385202 4_1:+45543808 Msa0550130:CDS
GGTGGTAGGGACCGTGGATA+TGG 0.392248 4_1:+45544478 Msa0550130:CDS
GGTGGGTATGGTGGAGGTGG+TGG 0.400681 4_1:+45544319 Msa0550130:CDS
GGTGGCGGCGGCGGTGGGTA+TGG 0.403563 4_1:+45544307 Msa0550130:CDS
TGTTCTCATGTTGATTTAGT+TGG 0.409418 4_1:+45544633 Msa0550130:three_prime_UTR
CGTGGATATGGTGGTGGAGG+TGG 0.410093 4_1:+45544490 Msa0550130:CDS
GGTGGTGGTGGTTATGGTGG+AGG 0.410493 4_1:+45544457 Msa0550130:CDS
AGAAAATTAGGTCTTCAGTT+TGG 0.418164 4_1:+45544593 Msa0550130:exon
GGAGGTGGTGGTTATGGAGG+TGG 0.419282 4_1:+45544331 Msa0550130:CDS
GGCGGCGGCGGTGGGTATGG+TGG 0.419877 4_1:+45544310 Msa0550130:CDS
CGTGGATATGGTGGAGGTGG+TGG 0.422932 4_1:+45544442 Msa0550130:CDS
GGCGGTGGTGGATATGGTGG+AGG 0.424937 4_1:+45544352 Msa0550130:CDS
GGCCACCGATAGCCAAGCTC+TGG 0.439871 4_1:+45543829 Msa0550130:CDS
GGTGGAGGTGGTGGTTATGG+AGG 0.440305 4_1:+45544328 Msa0550130:CDS
CAGTCACGTGGCAGTGGTGG+AGG 0.442750 4_1:+45544277 Msa0550130:CDS
GGCAGTGGTGGAGGAGGTAG+AGG 0.451811 4_1:+45544286 Msa0550130:CDS
GGTGGTGGAGGATATGGTGG+AGG 0.455686 4_1:+45544412 Msa0550130:CDS
GGCGGTGGGTATGGTGGAGG+TGG 0.455761 4_1:+45544316 Msa0550130:CDS
GGTGGTGGATATGGTGGAGG+TGG 0.459945 4_1:+45544355 Msa0550130:CDS
GGTGGATATGGTGGAGGTGG+TGG 0.461684 4_1:+45544358 Msa0550130:CDS
GGAGGTGGTGGTCGTCGTGA+TGG 0.466320 4_1:+45544370 Msa0550130:CDS
CGCGGTGGTGGTGCTGTTAG+CGG 0.467379 4_1:+45544553 Msa0550130:CDS
AGCAGAAGTGGTGGTGGTGG+AGG 0.470138 4_1:+45544400 Msa0550130:CDS
TGGTTATGGTGGAGGTGGTA+GGG 0.472480 4_1:+45544465 Msa0550130:CDS
GGCGGCGGTGGGTATGGTGG+AGG 0.475186 4_1:+45544313 Msa0550130:CDS
CGTGGTTATGGTGGTGGTGG+CGG 0.475722 4_1:+45544517 Msa0550130:CDS
GGATATGGTGGAGGTGGTGG+TGG 0.477834 4_1:+45544445 Msa0550130:CDS
AGAAGGCTTGCTCCAGAGCT+TGG 0.478074 4_1:-45543841 None:intergenic
GTAGAGGTGGCGGCGGCGGT+GGG 0.479681 4_1:+45544302 Msa0550130:CDS
GGTGGTGGTTATGGAGGTGG+CGG 0.480019 4_1:+45544334 Msa0550130:CDS
GGTGGAGGATATGGTGGAGG+TGG 0.483928 4_1:+45544415 Msa0550130:CDS
GGAGGAGGTAGAGGTGGCGG+CGG 0.497158 4_1:+45544295 Msa0550130:CDS
ATGCAATTGAAGAGATGAAT+GGG 0.497511 4_1:+45544215 Msa0550130:CDS
GGTGGTGGTGGAGGATATGG+TGG 0.497601 4_1:+45544409 Msa0550130:CDS
GGTGGTGGTTATGGTGGAGG+TGG 0.498780 4_1:+45544460 Msa0550130:CDS
TACAGCAGAAGTGGTGGTGG+TGG 0.500718 4_1:+45544397 Msa0550130:CDS
AAATTAGGTCTTCAGTTTGG+TGG 0.507215 4_1:+45544596 Msa0550130:three_prime_UTR
CAAGACCACCGACGAAGCAC+CGG 0.514431 4_1:-45543802 None:intergenic
GACCGTGGTTATGGTGGTGG+TGG 0.516242 4_1:+45544514 Msa0550130:CDS
GATCGCTACTCTCGCGGTGG+TGG 0.518871 4_1:+45544541 Msa0550130:CDS
GCTCAGTCACGTGGCAGTGG+TGG 0.524094 4_1:+45544274 Msa0550130:CDS
GGTGGAGGAGGTAGAGGTGG+CGG 0.531842 4_1:+45544292 Msa0550130:CDS
GATGCAATTGAAGAGATGAA+TGG 0.533095 4_1:+45544214 Msa0550130:CDS
TCACGTGGCAGTGGTGGAGG+AGG 0.534421 4_1:+45544280 Msa0550130:CDS
GACCGTGGATATGGTGGAGG+TGG 0.539518 4_1:+45544439 Msa0550130:CDS
GAAGCTCAGTCACGTGGCAG+TGG 0.541791 4_1:+45544271 Msa0550130:CDS
GACCGTGGATATGGTGGTGG+AGG 0.547656 4_1:+45544487 Msa0550130:CDS
AGGGACCGTGGATATGGTGG+TGG 0.548183 4_1:+45544484 Msa0550130:CDS
AGAACCCATCGAACTATTAG+CGG 0.549643 4_1:-45543765 None:intergenic
TGGTGAAATCACTGATTCCA+AGG 0.553599 4_1:+45543871 Msa0550130:CDS
TTGCTCCAGAGCTTGGCTAT+CGG 0.556107 4_1:-45543834 None:intergenic
GGTGACCGTGGTTATGGTGG+TGG 0.556613 4_1:+45544511 Msa0550130:CDS
GGTGGTGACCGTGGATATGG+TGG 0.557481 4_1:+45544433 Msa0550130:CDS
GGTGGTGGTCGTCGTGATGG+TGG 0.559431 4_1:+45544373 Msa0550130:CDS
GGCGGTGATCGCTACTCTCG+CGG 0.560534 4_1:+45544535 Msa0550130:CDS
CGTGAAACTGGAAGATCAAG+AGG 0.562850 4_1:+45544151 Msa0550130:CDS
AGTGGTGGAGGAGGTAGAGG+TGG 0.566280 4_1:+45544289 Msa0550130:CDS
ATGAATGGGCAGGATATTGA+CGG 0.571190 4_1:+45544229 Msa0550130:CDS
CTCCAGAGCTTGGCTATCGG+TGG 0.571940 4_1:-45543831 None:intergenic
GGTGGTGACCGTGGTTATGG+TGG 0.575688 4_1:+45544508 Msa0550130:CDS
GATTTCACCAAACTTAGAGA+AGG 0.575852 4_1:-45543858 None:intergenic
GGTGGAGGTGGTAGGGACCG+TGG 0.578195 4_1:+45544472 Msa0550130:CDS
GGTGACCGTGGATATGGTGG+AGG 0.581643 4_1:+45544436 Msa0550130:CDS
GGATACAGCAGAAGTGGTGG+TGG 0.583391 4_1:+45544394 Msa0550130:CDS
GGTAGGGACCGTGGATATGG+TGG 0.583912 4_1:+45544481 Msa0550130:CDS
GTTGAGTACCGGTGCTTCGT+CGG 0.588309 4_1:+45543794 Msa0550130:CDS
AATTGAAGAGATGAATGGGC+AGG 0.591467 4_1:+45544219 Msa0550130:CDS
CTGTTAGCGGTGGAAACTGG+AGG 0.597347 4_1:+45544566 Msa0550130:CDS
ATGGGCAGGATATTGACGGC+AGG 0.611813 4_1:+45544233 Msa0550130:CDS
GAGTACCGGTGCTTCGTCGG+TGG 0.614835 4_1:+45543797 Msa0550130:CDS
GTTCTTCTGATGTTGAGTAC+CGG 0.622558 4_1:+45543783 Msa0550130:CDS
GGTGGTGGAGGTGGTGACCG+TGG 0.625086 4_1:+45544499 Msa0550130:CDS
CACCACCTCCACCATATCCA+CGG 0.630646 4_1:-45544441 None:intergenic
CACCTCCACCACCATATCCA+CGG 0.630646 4_1:-45544489 None:intergenic
TATGGTGGAGGTGGTGACCG+TGG 0.638935 4_1:+45544424 Msa0550130:CDS
TGTTAGCGGTGGAAACTGGA+GGG 0.652063 4_1:+45544567 Msa0550130:CDS
CGCCACCACCACCATAACCA+CGG 0.664909 4_1:-45544516 None:intergenic
GATGGTGGATACAGCAGAAG+TGG 0.665118 4_1:+45544388 Msa0550130:CDS
GTGAATGAAGCTCAGTCACG+TGG 0.667871 4_1:+45544265 Msa0550130:CDS
GGTGGTGGTGCTGTTAGCGG+TGG 0.679424 4_1:+45544556 Msa0550130:CDS
GGTGATCGCTACTCTCGCGG+TGG 0.687217 4_1:+45544538 Msa0550130:CDS
GGTGGATACAGCAGAAGTGG+TGG 0.687566 4_1:+45544391 Msa0550130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAAACGATTGATTAAAA+AGG + chr4_1:45544736-45544755 Msa0550130:three_prime_UTR 20.0%
!! TAAAAAGGAGAATGAAAAAT+TGG + chr4_1:45544751-45544770 Msa0550130:three_prime_UTR 20.0%
!! GTATAAACGATTGATTAAAA+AGG + chr4_1:45544736-45544755 Msa0550130:three_prime_UTR 20.0%
!! TAAAAAGGAGAATGAAAAAT+TGG + chr4_1:45544751-45544770 Msa0550130:three_prime_UTR 20.0%
TTAGCGGAAAAAAAAGATGA+AGG - chr4_1:45543752-45543771 None:intergenic 30.0%
! TGTGAATTTTCTTAGATCTG+AGG + chr4_1:45544102-45544121 Msa0550130:intron 30.0%
!!! ATCTGAGGTTATTTTTGTTC+AGG + chr4_1:45544117-45544136 Msa0550130:intron 30.0%
ATGCAATTGAAGAGATGAAT+GGG + chr4_1:45544215-45544234 Msa0550130:CDS 30.0%
AGAAAATTAGGTCTTCAGTT+TGG + chr4_1:45544593-45544612 Msa0550130:exon 30.0%
!! TGTTCTCATGTTGATTTAGT+TGG + chr4_1:45544633-45544652 Msa0550130:three_prime_UTR 30.0%
TTGGTTGAAGTTTAGTGATT+TGG + chr4_1:45544652-45544671 Msa0550130:three_prime_UTR 30.0%
GTTTATACAAAACCAGATTC+AGG - chr4_1:45544724-45544743 None:intergenic 30.0%
TTAGCGGAAAAAAAAGATGA+AGG - chr4_1:45543752-45543771 None:intergenic 30.0%
! TGTGAATTTTCTTAGATCTG+AGG + chr4_1:45544102-45544121 Msa0550130:intron 30.0%
!!! ATCTGAGGTTATTTTTGTTC+AGG + chr4_1:45544117-45544136 Msa0550130:intron 30.0%
ATGCAATTGAAGAGATGAAT+GGG + chr4_1:45544215-45544234 Msa0550130:CDS 30.0%
AGAAAATTAGGTCTTCAGTT+TGG + chr4_1:45544593-45544612 Msa0550130:exon 30.0%
!! TGTTCTCATGTTGATTTAGT+TGG + chr4_1:45544633-45544652 Msa0550130:three_prime_UTR 30.0%
TTGGTTGAAGTTTAGTGATT+TGG + chr4_1:45544652-45544671 Msa0550130:three_prime_UTR 30.0%
GTTTATACAAAACCAGATTC+AGG - chr4_1:45544724-45544743 None:intergenic 30.0%
! TTTTTCCGCTAATAGTTCGA+TGG + chr4_1:45543760-45543779 Msa0550130:exon 35.0%
! TTTTCCGCTAATAGTTCGAT+GGG + chr4_1:45543761-45543780 Msa0550130:exon 35.0%
GATTTCACCAAACTTAGAGA+AGG - chr4_1:45543861-45543880 None:intergenic 35.0%
! CTTTTGTTACTCACACCAAT+AGG + chr4_1:45543998-45544017 Msa0550130:intron 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_1:45544016-45544035 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_1:45544042-45544061 Msa0550130:intron 35.0%
! AAGATCGATCGGTTTCTATT+CGG + chr4_1:45544053-45544072 Msa0550130:intron 35.0%
GTTATCAATGATCGTGAAAC+TGG + chr4_1:45544139-45544158 Msa0550130:CDS 35.0%
GATGCAATTGAAGAGATGAA+TGG + chr4_1:45544214-45544233 Msa0550130:CDS 35.0%
ACTGGAGGGATTAGAAAATT+AGG + chr4_1:45544581-45544600 Msa0550130:exon 35.0%
AAATTAGGTCTTCAGTTTGG+TGG + chr4_1:45544596-45544615 Msa0550130:three_prime_UTR 35.0%
! TTTTTCCGCTAATAGTTCGA+TGG + chr4_1:45543760-45543779 Msa0550130:exon 35.0%
! TTTTCCGCTAATAGTTCGAT+GGG + chr4_1:45543761-45543780 Msa0550130:exon 35.0%
GATTTCACCAAACTTAGAGA+AGG - chr4_1:45543861-45543880 None:intergenic 35.0%
! CTTTTGTTACTCACACCAAT+AGG + chr4_1:45543998-45544017 Msa0550130:intron 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_1:45544016-45544035 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_1:45544042-45544061 Msa0550130:intron 35.0%
! AAGATCGATCGGTTTCTATT+CGG + chr4_1:45544053-45544072 Msa0550130:intron 35.0%
GTTATCAATGATCGTGAAAC+TGG + chr4_1:45544139-45544158 Msa0550130:CDS 35.0%
GATGCAATTGAAGAGATGAA+TGG + chr4_1:45544214-45544233 Msa0550130:CDS 35.0%
ACTGGAGGGATTAGAAAATT+AGG + chr4_1:45544581-45544600 Msa0550130:exon 35.0%
AAATTAGGTCTTCAGTTTGG+TGG + chr4_1:45544596-45544615 Msa0550130:three_prime_UTR 35.0%
AGAACCCATCGAACTATTAG+CGG - chr4_1:45543768-45543787 None:intergenic 40.0%
GTTCTTCTGATGTTGAGTAC+CGG + chr4_1:45543783-45543802 Msa0550130:CDS 40.0%
!! TGGTGAAATCACTGATTCCA+AGG + chr4_1:45543871-45543890 Msa0550130:CDS 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_1:45544046-45544065 None:intergenic 40.0%
!! GATCGGTTTCTATTCGGTTT+TGG + chr4_1:45544059-45544078 Msa0550130:intron 40.0%
! ACTGGAAGATCAAGAGGTTT+TGG + chr4_1:45544157-45544176 Msa0550130:CDS 40.0%
AATTGAAGAGATGAATGGGC+AGG + chr4_1:45544219-45544238 Msa0550130:CDS 40.0%
ATGAATGGGCAGGATATTGA+CGG + chr4_1:45544229-45544248 Msa0550130:CDS 40.0%
!! TGTCTTTTTCGTCCTGAATC+TGG + chr4_1:45544709-45544728 Msa0550130:three_prime_UTR 40.0%
! ATTTTGAGCAGTGAACTCAG+AGG - chr4_1:45543724-45543743 None:intergenic 40.0%
AGAACCCATCGAACTATTAG+CGG - chr4_1:45543768-45543787 None:intergenic 40.0%
GTTCTTCTGATGTTGAGTAC+CGG + chr4_1:45543783-45543802 Msa0550130:CDS 40.0%
!! TGGTGAAATCACTGATTCCA+AGG + chr4_1:45543871-45543890 Msa0550130:CDS 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_1:45544046-45544065 None:intergenic 40.0%
!! GATCGGTTTCTATTCGGTTT+TGG + chr4_1:45544059-45544078 Msa0550130:intron 40.0%
! ACTGGAAGATCAAGAGGTTT+TGG + chr4_1:45544157-45544176 Msa0550130:CDS 40.0%
AATTGAAGAGATGAATGGGC+AGG + chr4_1:45544219-45544238 Msa0550130:CDS 40.0%
ATGAATGGGCAGGATATTGA+CGG + chr4_1:45544229-45544248 Msa0550130:CDS 40.0%
!! TGTCTTTTTCGTCCTGAATC+TGG + chr4_1:45544709-45544728 Msa0550130:three_prime_UTR 40.0%
GAGCAAGCCTTCTCTAAGTT+TGG + chr4_1:45543851-45543870 Msa0550130:CDS 45.0%
AATCCGAATCAGAATCCGCA+CGG - chr4_1:45543898-45543917 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_1:45544151-45544170 Msa0550130:CDS 45.0%
GAGCAAGCCTTCTCTAAGTT+TGG + chr4_1:45543851-45543870 Msa0550130:CDS 45.0%
AATCCGAATCAGAATCCGCA+CGG - chr4_1:45543898-45543917 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_1:45544151-45544170 Msa0550130:CDS 45.0%
TTGCTCCAGAGCTTGGCTAT+CGG - chr4_1:45543837-45543856 None:intergenic 50.0%
GTGAATGAAGCTCAGTCACG+TGG + chr4_1:45544265-45544284 Msa0550130:CDS 50.0%
! TATGGTGGAGGTGGTGGTTA+TGG + chr4_1:45544325-45544344 Msa0550130:CDS 50.0%
!! GATGGTGGATACAGCAGAAG+TGG + chr4_1:45544388-45544407 Msa0550130:CDS 50.0%
! TGGTTATGGTGGAGGTGGTA+GGG + chr4_1:45544465-45544484 Msa0550130:CDS 50.0%
TGTTAGCGGTGGAAACTGGA+GGG + chr4_1:45544567-45544586 Msa0550130:CDS 50.0%
TTGCTCCAGAGCTTGGCTAT+CGG - chr4_1:45543837-45543856 None:intergenic 50.0%
GTGAATGAAGCTCAGTCACG+TGG + chr4_1:45544265-45544284 Msa0550130:CDS 50.0%
! TATGGTGGAGGTGGTGGTTA+TGG + chr4_1:45544325-45544344 Msa0550130:CDS 50.0%
!! GATGGTGGATACAGCAGAAG+TGG + chr4_1:45544388-45544407 Msa0550130:CDS 50.0%
! TGGTTATGGTGGAGGTGGTA+GGG + chr4_1:45544465-45544484 Msa0550130:CDS 50.0%
TGTTAGCGGTGGAAACTGGA+GGG + chr4_1:45544567-45544586 Msa0550130:CDS 50.0%
!! GTTGAGTACCGGTGCTTCGT+CGG + chr4_1:45543794-45543813 Msa0550130:CDS 55.0%
! CTTCGTCGGTGGTCTTGCTT+GGG + chr4_1:45543808-45543827 Msa0550130:CDS 55.0%
! AGAAGGCTTGCTCCAGAGCT+TGG - chr4_1:45543844-45543863 None:intergenic 55.0%
!! CACTGATTCCAAGGTCCGTG+CGG + chr4_1:45543880-45543899 Msa0550130:intron 55.0%
ATCAGAATCCGCACGGACCT+TGG - chr4_1:45543891-45543910 None:intergenic 55.0%
GGTCCGTGCGGATTCTGATT+CGG + chr4_1:45543892-45543911 Msa0550130:intron 55.0%
ATGGGCAGGATATTGACGGC+AGG + chr4_1:45544233-45544252 Msa0550130:CDS 55.0%
GGTGGATACAGCAGAAGTGG+TGG + chr4_1:45544391-45544410 Msa0550130:CDS 55.0%
GGATACAGCAGAAGTGGTGG+TGG + chr4_1:45544394-45544413 Msa0550130:CDS 55.0%
!! TACAGCAGAAGTGGTGGTGG+TGG + chr4_1:45544397-45544416 Msa0550130:CDS 55.0%
!! AGTGGTGGTGGTGGAGGATA+TGG + chr4_1:45544406-45544425 Msa0550130:CDS 55.0%
CACCACCTCCACCATATCCA+CGG - chr4_1:45544360-45544379 None:intergenic 55.0%
! GTGGTTATGGTGGAGGTGGT+AGG + chr4_1:45544464-45544483 Msa0550130:CDS 55.0%
CACCTCCACCACCATATCCA+CGG - chr4_1:45544492-45544511 None:intergenic 55.0%
! GTGCTGTTAGCGGTGGAAAC+TGG + chr4_1:45544563-45544582 Msa0550130:CDS 55.0%
CTGTTAGCGGTGGAAACTGG+AGG + chr4_1:45544566-45544585 Msa0550130:CDS 55.0%
!! GTTGAGTACCGGTGCTTCGT+CGG + chr4_1:45543794-45543813 Msa0550130:CDS 55.0%
! CTTCGTCGGTGGTCTTGCTT+GGG + chr4_1:45543808-45543827 Msa0550130:CDS 55.0%
! AGAAGGCTTGCTCCAGAGCT+TGG - chr4_1:45543844-45543863 None:intergenic 55.0%
!! CACTGATTCCAAGGTCCGTG+CGG + chr4_1:45543880-45543899 Msa0550130:intron 55.0%
ATCAGAATCCGCACGGACCT+TGG - chr4_1:45543891-45543910 None:intergenic 55.0%
GGTCCGTGCGGATTCTGATT+CGG + chr4_1:45543892-45543911 Msa0550130:intron 55.0%
ATGGGCAGGATATTGACGGC+AGG + chr4_1:45544233-45544252 Msa0550130:CDS 55.0%
GGTGGATACAGCAGAAGTGG+TGG + chr4_1:45544391-45544410 Msa0550130:CDS 55.0%
GGATACAGCAGAAGTGGTGG+TGG + chr4_1:45544394-45544413 Msa0550130:CDS 55.0%
!! TACAGCAGAAGTGGTGGTGG+TGG + chr4_1:45544397-45544416 Msa0550130:CDS 55.0%
!! AGTGGTGGTGGTGGAGGATA+TGG + chr4_1:45544406-45544425 Msa0550130:CDS 55.0%
CACCACCTCCACCATATCCA+CGG - chr4_1:45544360-45544379 None:intergenic 55.0%
! GTGGTTATGGTGGAGGTGGT+AGG + chr4_1:45544464-45544483 Msa0550130:CDS 55.0%
CACCTCCACCACCATATCCA+CGG - chr4_1:45544492-45544511 None:intergenic 55.0%
! GTGCTGTTAGCGGTGGAAAC+TGG + chr4_1:45544563-45544582 Msa0550130:CDS 55.0%
CTGTTAGCGGTGGAAACTGG+AGG + chr4_1:45544566-45544585 Msa0550130:CDS 55.0%
CAAGACCACCGACGAAGCAC+CGG - chr4_1:45543805-45543824 None:intergenic 60.0%
! GCTTCGTCGGTGGTCTTGCT+TGG + chr4_1:45543807-45543826 Msa0550130:CDS 60.0%
CTCCAGAGCTTGGCTATCGG+TGG - chr4_1:45543834-45543853 None:intergenic 60.0%
GAAGCTCAGTCACGTGGCAG+TGG + chr4_1:45544271-45544290 Msa0550130:CDS 60.0%
! AGTGGTGGAGGAGGTAGAGG+TGG + chr4_1:45544289-45544308 Msa0550130:CDS 60.0%
! GGTGGAGGTGGTGGTTATGG+AGG + chr4_1:45544328-45544347 Msa0550130:CDS 60.0%
! GGAGGTGGTGGTTATGGAGG+TGG + chr4_1:45544331-45544350 Msa0550130:CDS 60.0%
! GGTGGTGGTTATGGAGGTGG+CGG + chr4_1:45544334-45544353 Msa0550130:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + chr4_1:45544355-45544374 Msa0550130:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_1:45544358-45544377 Msa0550130:CDS 60.0%
!! AGCAGAAGTGGTGGTGGTGG+AGG + chr4_1:45544400-45544419 Msa0550130:CDS 60.0%
!! GGTGGTGGTGGAGGATATGG+TGG + chr4_1:45544409-45544428 Msa0550130:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+AGG + chr4_1:45544412-45544431 Msa0550130:CDS 60.0%
! GGTGGAGGATATGGTGGAGG+TGG + chr4_1:45544415-45544434 Msa0550130:CDS 60.0%
! TATGGTGGAGGTGGTGACCG+TGG + chr4_1:45544424-45544443 Msa0550130:CDS 60.0%
GGAGGTGGTGACCGTGGATA+TGG + chr4_1:45544430-45544449 Msa0550130:CDS 60.0%
GGTGGTGACCGTGGATATGG+TGG + chr4_1:45544433-45544452 Msa0550130:CDS 60.0%
GGTGACCGTGGATATGGTGG+AGG + chr4_1:45544436-45544455 Msa0550130:CDS 60.0%
! GACCGTGGATATGGTGGAGG+TGG + chr4_1:45544439-45544458 Msa0550130:CDS 60.0%
! CGTGGATATGGTGGAGGTGG+TGG + chr4_1:45544442-45544461 Msa0550130:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG + chr4_1:45544361-45544380 Msa0550130:CDS 60.0%
!! GGTGGAGGTGGTGGTGGTTA+TGG + chr4_1:45544451-45544470 Msa0550130:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_1:45544454-45544473 Msa0550130:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+AGG + chr4_1:45544457-45544476 Msa0550130:CDS 60.0%
! GGTGGTGGTTATGGTGGAGG+TGG + chr4_1:45544460-45544479 Msa0550130:CDS 60.0%
GGTGGTAGGGACCGTGGATA+TGG + chr4_1:45544478-45544497 Msa0550130:CDS 60.0%
GGTAGGGACCGTGGATATGG+TGG + chr4_1:45544481-45544500 Msa0550130:CDS 60.0%
AGGGACCGTGGATATGGTGG+TGG + chr4_1:45544484-45544503 Msa0550130:CDS 60.0%
!! GACCGTGGATATGGTGGTGG+AGG + chr4_1:45544487-45544506 Msa0550130:CDS 60.0%
!! CGTGGATATGGTGGTGGAGG+TGG + chr4_1:45544490-45544509 Msa0550130:CDS 60.0%
GGAGGTGGTGACCGTGGTTA+TGG + chr4_1:45544505-45544524 Msa0550130:CDS 60.0%
GGTGGTGACCGTGGTTATGG+TGG + chr4_1:45544508-45544527 Msa0550130:CDS 60.0%
GGTGACCGTGGTTATGGTGG+TGG + chr4_1:45544511-45544530 Msa0550130:CDS 60.0%
!! GACCGTGGTTATGGTGGTGG+TGG + chr4_1:45544514-45544533 Msa0550130:CDS 60.0%
CGCCACCACCACCATAACCA+CGG - chr4_1:45544519-45544538 None:intergenic 60.0%
!! CGTGGTTATGGTGGTGGTGG+CGG + chr4_1:45544517-45544536 Msa0550130:CDS 60.0%
CAAGACCACCGACGAAGCAC+CGG - chr4_1:45543805-45543824 None:intergenic 60.0%
! GCTTCGTCGGTGGTCTTGCT+TGG + chr4_1:45543807-45543826 Msa0550130:CDS 60.0%
CTCCAGAGCTTGGCTATCGG+TGG - chr4_1:45543834-45543853 None:intergenic 60.0%
GAAGCTCAGTCACGTGGCAG+TGG + chr4_1:45544271-45544290 Msa0550130:CDS 60.0%
! AGTGGTGGAGGAGGTAGAGG+TGG + chr4_1:45544289-45544308 Msa0550130:CDS 60.0%
! GGTGGAGGTGGTGGTTATGG+AGG + chr4_1:45544328-45544347 Msa0550130:CDS 60.0%
! GGAGGTGGTGGTTATGGAGG+TGG + chr4_1:45544331-45544350 Msa0550130:CDS 60.0%
! GGTGGTGGTTATGGAGGTGG+CGG + chr4_1:45544334-45544353 Msa0550130:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + chr4_1:45544355-45544374 Msa0550130:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_1:45544358-45544377 Msa0550130:CDS 60.0%
!! AGCAGAAGTGGTGGTGGTGG+AGG + chr4_1:45544400-45544419 Msa0550130:CDS 60.0%
!! GGTGGTGGTGGAGGATATGG+TGG + chr4_1:45544409-45544428 Msa0550130:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+AGG + chr4_1:45544412-45544431 Msa0550130:CDS 60.0%
! GGTGGAGGATATGGTGGAGG+TGG + chr4_1:45544415-45544434 Msa0550130:CDS 60.0%
! TATGGTGGAGGTGGTGACCG+TGG + chr4_1:45544424-45544443 Msa0550130:CDS 60.0%
GGAGGTGGTGACCGTGGATA+TGG + chr4_1:45544430-45544449 Msa0550130:CDS 60.0%
GGTGGTGACCGTGGATATGG+TGG + chr4_1:45544433-45544452 Msa0550130:CDS 60.0%
GGTGACCGTGGATATGGTGG+AGG + chr4_1:45544436-45544455 Msa0550130:CDS 60.0%
! GACCGTGGATATGGTGGAGG+TGG + chr4_1:45544439-45544458 Msa0550130:CDS 60.0%
! CGTGGATATGGTGGAGGTGG+TGG + chr4_1:45544442-45544461 Msa0550130:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG + chr4_1:45544361-45544380 Msa0550130:CDS 60.0%
!! GGTGGAGGTGGTGGTGGTTA+TGG + chr4_1:45544451-45544470 Msa0550130:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_1:45544454-45544473 Msa0550130:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+AGG + chr4_1:45544457-45544476 Msa0550130:CDS 60.0%
! GGTGGTGGTTATGGTGGAGG+TGG + chr4_1:45544460-45544479 Msa0550130:CDS 60.0%
GGTGGTAGGGACCGTGGATA+TGG + chr4_1:45544478-45544497 Msa0550130:CDS 60.0%
GGTAGGGACCGTGGATATGG+TGG + chr4_1:45544481-45544500 Msa0550130:CDS 60.0%
AGGGACCGTGGATATGGTGG+TGG + chr4_1:45544484-45544503 Msa0550130:CDS 60.0%
!! GACCGTGGATATGGTGGTGG+AGG + chr4_1:45544487-45544506 Msa0550130:CDS 60.0%
!! CGTGGATATGGTGGTGGAGG+TGG + chr4_1:45544490-45544509 Msa0550130:CDS 60.0%
GGAGGTGGTGACCGTGGTTA+TGG + chr4_1:45544505-45544524 Msa0550130:CDS 60.0%
GGTGGTGACCGTGGTTATGG+TGG + chr4_1:45544508-45544527 Msa0550130:CDS 60.0%
GGTGACCGTGGTTATGGTGG+TGG + chr4_1:45544511-45544530 Msa0550130:CDS 60.0%
!! GACCGTGGTTATGGTGGTGG+TGG + chr4_1:45544514-45544533 Msa0550130:CDS 60.0%
CGCCACCACCACCATAACCA+CGG - chr4_1:45544519-45544538 None:intergenic 60.0%
!! CGTGGTTATGGTGGTGGTGG+CGG + chr4_1:45544517-45544536 Msa0550130:CDS 60.0%
!! GAGTACCGGTGCTTCGTCGG+TGG + chr4_1:45543797-45543816 Msa0550130:CDS 65.0%
GGCCACCGATAGCCAAGCTC+TGG + chr4_1:45543829-45543848 Msa0550130:CDS 65.0%
GCTCAGTCACGTGGCAGTGG+TGG + chr4_1:45544274-45544293 Msa0550130:CDS 65.0%
CAGTCACGTGGCAGTGGTGG+AGG + chr4_1:45544277-45544296 Msa0550130:CDS 65.0%
! TCACGTGGCAGTGGTGGAGG+AGG + chr4_1:45544280-45544299 Msa0550130:CDS 65.0%
! GGCAGTGGTGGAGGAGGTAG+AGG + chr4_1:45544286-45544305 Msa0550130:CDS 65.0%
GGTGGAGGAGGTAGAGGTGG+CGG + chr4_1:45544292-45544311 Msa0550130:CDS 65.0%
! GGTGGGTATGGTGGAGGTGG+TGG + chr4_1:45544319-45544338 Msa0550130:CDS 65.0%
! GGTGGTTATGGAGGTGGCGG+TGG + chr4_1:45544337-45544356 Msa0550130:CDS 65.0%
! GGTTATGGAGGTGGCGGTGG+TGG + chr4_1:45544340-45544359 Msa0550130:CDS 65.0%
!! GGAGGTGGCGGTGGTGGATA+TGG + chr4_1:45544346-45544365 Msa0550130:CDS 65.0%
!! GGTGGCGGTGGTGGATATGG+TGG + chr4_1:45544349-45544368 Msa0550130:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+AGG + chr4_1:45544352-45544371 Msa0550130:CDS 65.0%
! GGAGGTGGTGGTCGTCGTGA+TGG + chr4_1:45544370-45544389 Msa0550130:CDS 65.0%
! GGTGGTGGTCGTCGTGATGG+TGG + chr4_1:45544373-45544392 Msa0550130:CDS 65.0%
GGCGGTGATCGCTACTCTCG+CGG + chr4_1:45544535-45544554 Msa0550130:CDS 65.0%
GGTGATCGCTACTCTCGCGG+TGG + chr4_1:45544538-45544557 Msa0550130:CDS 65.0%
GATCGCTACTCTCGCGGTGG+TGG + chr4_1:45544541-45544560 Msa0550130:CDS 65.0%
!! CGCGGTGGTGGTGCTGTTAG+CGG + chr4_1:45544553-45544572 Msa0550130:CDS 65.0%
!! GGTGGTGGTGCTGTTAGCGG+TGG + chr4_1:45544556-45544575 Msa0550130:CDS 65.0%
!! GAGTACCGGTGCTTCGTCGG+TGG + chr4_1:45543797-45543816 Msa0550130:CDS 65.0%
GGCCACCGATAGCCAAGCTC+TGG + chr4_1:45543829-45543848 Msa0550130:CDS 65.0%
GCTCAGTCACGTGGCAGTGG+TGG + chr4_1:45544274-45544293 Msa0550130:CDS 65.0%
CAGTCACGTGGCAGTGGTGG+AGG + chr4_1:45544277-45544296 Msa0550130:CDS 65.0%
! TCACGTGGCAGTGGTGGAGG+AGG + chr4_1:45544280-45544299 Msa0550130:CDS 65.0%
! GGCAGTGGTGGAGGAGGTAG+AGG + chr4_1:45544286-45544305 Msa0550130:CDS 65.0%
GGTGGAGGAGGTAGAGGTGG+CGG + chr4_1:45544292-45544311 Msa0550130:CDS 65.0%
! GGTGGGTATGGTGGAGGTGG+TGG + chr4_1:45544319-45544338 Msa0550130:CDS 65.0%
! GGTGGTTATGGAGGTGGCGG+TGG + chr4_1:45544337-45544356 Msa0550130:CDS 65.0%
! GGTTATGGAGGTGGCGGTGG+TGG + chr4_1:45544340-45544359 Msa0550130:CDS 65.0%
!! GGAGGTGGCGGTGGTGGATA+TGG + chr4_1:45544346-45544365 Msa0550130:CDS 65.0%
!! GGTGGCGGTGGTGGATATGG+TGG + chr4_1:45544349-45544368 Msa0550130:CDS 65.0%
!! GGCGGTGGTGGATATGGTGG+AGG + chr4_1:45544352-45544371 Msa0550130:CDS 65.0%
! GGAGGTGGTGGTCGTCGTGA+TGG + chr4_1:45544370-45544389 Msa0550130:CDS 65.0%
! GGTGGTGGTCGTCGTGATGG+TGG + chr4_1:45544373-45544392 Msa0550130:CDS 65.0%
GGCGGTGATCGCTACTCTCG+CGG + chr4_1:45544535-45544554 Msa0550130:CDS 65.0%
GGTGATCGCTACTCTCGCGG+TGG + chr4_1:45544538-45544557 Msa0550130:CDS 65.0%
GATCGCTACTCTCGCGGTGG+TGG + chr4_1:45544541-45544560 Msa0550130:CDS 65.0%
!! CGCGGTGGTGGTGCTGTTAG+CGG + chr4_1:45544553-45544572 Msa0550130:CDS 65.0%
!! GGTGGTGGTGCTGTTAGCGG+TGG + chr4_1:45544556-45544575 Msa0550130:CDS 65.0%
! GGAGGAGGTAGAGGTGGCGG+CGG + chr4_1:45544295-45544314 Msa0550130:CDS 70.0%
! GGCGGTGGGTATGGTGGAGG+TGG + chr4_1:45544316-45544335 Msa0550130:CDS 70.0%
GGTGGAGGTGGTAGGGACCG+TGG + chr4_1:45544472-45544491 Msa0550130:CDS 70.0%
!! GGTGGTGGAGGTGGTGACCG+TGG + chr4_1:45544499-45544518 Msa0550130:CDS 70.0%
! GGAGGAGGTAGAGGTGGCGG+CGG + chr4_1:45544295-45544314 Msa0550130:CDS 70.0%
! GGCGGTGGGTATGGTGGAGG+TGG + chr4_1:45544316-45544335 Msa0550130:CDS 70.0%
GGTGGAGGTGGTAGGGACCG+TGG + chr4_1:45544472-45544491 Msa0550130:CDS 70.0%
!! GGTGGTGGAGGTGGTGACCG+TGG + chr4_1:45544499-45544518 Msa0550130:CDS 70.0%
! GGAGGTAGAGGTGGCGGCGG+CGG + chr4_1:45544298-45544317 Msa0550130:CDS 75.0%
! GTAGAGGTGGCGGCGGCGGT+GGG + chr4_1:45544302-45544321 Msa0550130:CDS 75.0%
GGCGGCGGTGGGTATGGTGG+AGG + chr4_1:45544313-45544332 Msa0550130:CDS 75.0%
! GGAGGTAGAGGTGGCGGCGG+CGG + chr4_1:45544298-45544317 Msa0550130:CDS 75.0%
! GTAGAGGTGGCGGCGGCGGT+GGG + chr4_1:45544302-45544321 Msa0550130:CDS 75.0%
GGCGGCGGTGGGTATGGTGG+AGG + chr4_1:45544313-45544332 Msa0550130:CDS 75.0%
!! GGTAGAGGTGGCGGCGGCGG+TGG + chr4_1:45544301-45544320 Msa0550130:CDS 80.0%
!! GGTGGCGGCGGCGGTGGGTA+TGG + chr4_1:45544307-45544326 Msa0550130:CDS 80.0%
! GGCGGCGGCGGTGGGTATGG+TGG + chr4_1:45544310-45544329 Msa0550130:CDS 80.0%
!! GGTAGAGGTGGCGGCGGCGG+TGG + chr4_1:45544301-45544320 Msa0550130:CDS 80.0%
!! GGTGGCGGCGGCGGTGGGTA+TGG + chr4_1:45544307-45544326 Msa0550130:CDS 80.0%
! GGCGGCGGCGGTGGGTATGG+TGG + chr4_1:45544310-45544329 Msa0550130:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 45543723 45544793 45543723 ID=Msa0550130;Name=Msa0550130
chr4_1 mRNA 45543723 45544793 45543723 ID=Msa0550130-mRNA-1;Parent=Msa0550130;Name=Msa0550130-mRNA-1;_AED=0.08;_eAED=0.08;_QI=56|1|1|1|1|1|2|199|189
chr4_1 exon 45543723 45543892 45543723 ID=Msa0550130-mRNA-1:exon:11992;Parent=Msa0550130-mRNA-1
chr4_1 exon 45544139 45544793 45544139 ID=Msa0550130-mRNA-1:exon:11993;Parent=Msa0550130-mRNA-1
chr4_1 five_prime_UTR 45543723 45543778 45543723 ID=Msa0550130-mRNA-1:five_prime_utr;Parent=Msa0550130-mRNA-1
chr4_1 CDS 45543779 45543892 45543779 ID=Msa0550130-mRNA-1:cds;Parent=Msa0550130-mRNA-1
chr4_1 CDS 45544139 45544594 45544139 ID=Msa0550130-mRNA-1:cds;Parent=Msa0550130-mRNA-1
chr4_1 three_prime_UTR 45544595 45544793 45544595 ID=Msa0550130-mRNA-1:three_prime_utr;Parent=Msa0550130-mRNA-1
Gene Sequence

>Msa0550130

ATGGGTTCTTCTGATGTTGAGTACCGGTGCTTCGTCGGTGGTCTTGCTTGGGCCACCGATAGCCAAGCTCTGGAGCAAGCCTTCTCTAAGTTTGGTGAAATCACTGATTCCAAGGTTATCAATGATCGTGAAACTGGAAGATCAAGAGGTTTTGGATTTGTGACATTCGCTGATGAGAAATCGATGAGAGATGCAATTGAAGAGATGAATGGGCAGGATATTGACGGCAGGAATATTACTGTGAATGAAGCTCAGTCACGTGGCAGTGGTGGAGGAGGTAGAGGTGGCGGCGGCGGTGGGTATGGTGGAGGTGGTGGTTATGGAGGTGGCGGTGGTGGATATGGTGGAGGTGGTGGTCGTCGTGATGGTGGATACAGCAGAAGTGGTGGTGGTGGAGGATATGGTGGAGGTGGTGACCGTGGATATGGTGGAGGTGGTGGTGGTTATGGTGGAGGTGGTAGGGACCGTGGATATGGTGGTGGAGGTGGTGACCGTGGTTATGGTGGTGGTGGCGGTGATCGCTACTCTCGCGGTGGTGGTGCTGTTAGCGGTGGAAACTGGAGGGATTAG
Protein sequence

>Msa0550130

MGSSDVEYRCFVGGLAWATDSQALEQAFSKFGEITDSKVINDRETGRSRGFGFVTFADEKSMRDAIEEMNGQDIDGRNITVNEAQSRGSGGGGRGGGGGGYGGGGGYGGGGGGYGGGGGRRDGGYSRSGGGGGYGGGGDRGYGGGGGGYGGGGRDRGYGGGGGDRGYGGGGGDRYSRGGGAVSGGNWRD*