AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0698310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0698310 MtrunA17_Chr4g0051591 97.899 238 5 0 1 238 1 238 3.22e-175 481
Msa0698310 MtrunA17_Chr2g0292941 68.776 237 73 1 1 237 1 236 1.05e-124 353
Msa0698310 MtrunA17_Chr4g0051581 80.952 147 28 0 1 147 1 147 8.96e-77 229
Msa0698310 MtrunA17_Chr4g0051571 78.161 87 19 0 151 237 10 96 2.66e-45 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0698310 AT2G34770.1 63.158 228 84 0 1 228 1 228 2.22e-108 312
Msa0698310 AT4G20870.2 61.603 237 90 1 1 237 1 236 1.07e-106 308
Msa0698310 AT4G20870.1 61.603 237 90 1 1 237 1 236 1.07e-106 308

Find 104 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGGTGATTCCAATTATT+TGG 0.142082 4_4:-68587958 Msa0698310:CDS
ATCAAATTTGATACATATTA+TGG 0.180771 4_4:+68585971 None:intergenic
ATGGGTTCATCAACCTATTA+TGG 0.183910 4_4:-68588233 Msa0698310:CDS
ATTATATGTAATCTAAATTA+AGG 0.210656 4_4:+68588770 None:intergenic
GGGACGACACAGTGGTAAAT+TGG 0.215450 4_4:+68589112 None:intergenic
GCAATTGGAACCCAAATAAT+TGG 0.230184 4_4:+68587947 None:intergenic
TTGGTGAAGATTATGATAAA+TGG 0.262773 4_4:-68588252 Msa0698310:CDS
AAAAGAGGGAGGTGAGGAAC+AGG 0.286347 4_4:+68587887 None:intergenic
GTGTCAAACGTGTTTATTAC+AGG 0.288300 4_4:+68588893 None:intergenic
TCAATTGCTCCTGCTGTGTT+TGG 0.295260 4_4:-68586850 Msa0698310:CDS
GTGGGTGATTCCAATTATTT+GGG 0.302503 4_4:-68587957 Msa0698310:CDS
TTGGCATCACTTCACCACTA+TGG 0.307245 4_4:-68586206 Msa0698310:CDS
TGAGATTTGCATTTGTTCAT+TGG 0.329247 4_4:-68586118 Msa0698310:three_prime_UTR
GGGCCTTCCTTGCCCATAAT+AGG 0.340890 4_4:+68588220 None:intergenic
ATGTTCAAACTAAGAGCTAT+TGG 0.342050 4_4:-68587802 Msa0698310:CDS
AGCTATCCATAGGGTGCTTA+TGG 0.348531 4_4:+68587744 None:intergenic
GAATAAACCCCTTGTTTGTC+AGG 0.349453 4_4:-68588500 Msa0698310:intron
TTTCTTTCATAGCAGTATCT+TGG 0.357754 4_4:-68588194 Msa0698310:CDS
TATATTTGAATTCTATTATG+AGG 0.359179 4_4:-68588831 Msa0698310:five_prime_UTR
TGTTCAAACTAAGAGCTATT+GGG 0.361825 4_4:-68587801 Msa0698310:CDS
AATTGTTACTTATCTACGTT+TGG 0.364138 4_4:+68586140 None:intergenic
TGAGTGAGTGGGGTATGTTT+TGG 0.371283 4_4:+68589090 None:intergenic
CATTTATAACTCTTTCAGAT+TGG 0.376626 4_4:-68587915 Msa0698310:CDS
TGTAATCTAAATTAAGGAAT+GGG 0.377357 4_4:+68588776 None:intergenic
TAAAATCTCAACAACTGAAT+TGG 0.379417 4_4:+68588697 None:intergenic
ATTTATAACTCTTTCAGATT+GGG 0.403789 4_4:-68587914 Msa0698310:CDS
AGGGTCCAGAGCCTTGGCTT+TGG 0.410334 4_4:-68586225 Msa0698310:CDS
CACTTCACCACTATGGGATA+AGG 0.426484 4_4:-68586199 Msa0698310:CDS
TGGGTTCATCAACCTATTAT+GGG 0.432385 4_4:-68588232 Msa0698310:CDS
AAAACGGTGCAATAAGTATT+CGG 0.433689 4_4:+68587832 None:intergenic
TGGTGAAGATTATGATAAAT+GGG 0.434108 4_4:-68588251 Msa0698310:CDS
ATTGCTCCTGCTGTGTTTGG+AGG 0.436809 4_4:-68586847 Msa0698310:CDS
ACCATTTGAATCATCACTAC+AGG 0.439539 4_4:-68586245 Msa0698310:CDS
TGATGCCAAAGCCAAGGCTC+TGG 0.469577 4_4:+68586220 None:intergenic
TTTGAACATGGAAAAGAAAA+CGG 0.472915 4_4:+68587816 None:intergenic
CTTTCATAGCAGTATCTTGG+AGG 0.476416 4_4:-68588191 Msa0698310:intron
CACTACAGGGTCCAGAGCCT+TGG 0.478105 4_4:-68586231 Msa0698310:CDS
TGGCATCACTTCACCACTAT+GGG 0.480111 4_4:-68586205 Msa0698310:CDS
ACACATTATTACTTGCATCA+TGG 0.488209 4_4:-68586793 Msa0698310:CDS
CTGTTCCGAACATGATGATC+TGG 0.488917 4_4:+68589030 None:intergenic
ATGTAATCTAAATTAAGGAA+TGG 0.489047 4_4:+68588775 None:intergenic
TGGTTATTTATAGCAAATTG+AGG 0.495536 4_4:-68586098 Msa0698310:three_prime_UTR
ACAAGTCTTAAGCTATCCAT+AGG 0.497021 4_4:+68587734 None:intergenic
TAAATGAAACAAGATGCAAT+TGG 0.498395 4_4:+68587932 None:intergenic
CCTCTGCACAGTCCTTTCTC+TGG 0.498899 4_4:-68585929 Msa0698310:three_prime_UTR
AATATACAAATACAGTATAC+AGG 0.499179 4_4:+68588848 None:intergenic
GGACGACACAGTGGTAAATT+GGG 0.507441 4_4:+68589113 None:intergenic
ACCTACATTAAGCAATCTAA+AGG 0.509852 4_4:+68585878 None:intergenic
ATGTGACAGGTTGGTCATCT+TGG 0.510797 4_4:-68588271 Msa0698310:intron
TCCTTTAGATTGCTTAATGT+AGG 0.511317 4_4:-68585879 None:intergenic
AGGAAGAAGTTACCAGAGAA+AGG 0.512038 4_4:+68585917 None:intergenic
CAACCTATTATGGGCAAGGA+AGG 0.526322 4_4:-68588223 Msa0698310:CDS
TAAGTCTCCCTCTTTGAAAG+AGG 0.530065 4_4:+68588669 None:intergenic
AAGGAAGAAGGAATGGTGGT+AGG 0.532611 4_4:+68585897 None:intergenic
TTAAGCAATCTAAAGGAAGA+AGG 0.534545 4_4:+68585885 None:intergenic
AACACAGCAGGAGCAATTGA+AGG 0.534894 4_4:+68586853 None:intergenic
TAGCCTCCCTCTTTCAAAGA+GGG 0.541468 4_4:-68588676 Msa0698310:five_prime_UTR
GGTGCTTATGGTGGCAACCA+TGG 0.543946 4_4:+68587756 None:intergenic
GCTATTAAAGCGGCTCCAGC+TGG 0.544240 4_4:+68587704 None:intergenic
ACATTGCATTATCTTCTCCA+TGG 0.544817 4_4:-68587773 Msa0698310:CDS
ATGCCGAGTAGCACGAAAAG+AGG 0.548185 4_4:+68587872 None:intergenic
TTTCACGCGAACTGCTTGGT+GGG 0.549072 4_4:-68587975 Msa0698310:CDS
GGTTCCTCCTCCATCAAAAG+TGG 0.554501 4_4:-68586166 Msa0698310:CDS
ATTAAAGCGGCTCCAGCTGG+TGG 0.557678 4_4:+68587707 None:intergenic
CAATCTAAAGGAAGAAGGAA+TGG 0.559858 4_4:+68585890 None:intergenic
AGTTGGTTACCTGACAAACA+AGG 0.563299 4_4:+68588491 None:intergenic
CTTCTCTTCTCAGATCTCCT+CGG 0.565161 4_4:-68588643 Msa0698310:intron
TTGCCACCATAAGCACCCTA+TGG 0.565998 4_4:-68587750 Msa0698310:CDS
AAGTCTCCCTCTTTGAAAGA+GGG 0.576055 4_4:+68588670 None:intergenic
AGCCGCTTTAATAGCCTACA+TGG 0.576385 4_4:-68587696 Msa0698310:intron
GTTCAAACTAAGAGCTATTG+GGG 0.580121 4_4:-68587800 Msa0698310:CDS
ATGAGAAAGATCATTGGCAC+AGG 0.580902 4_4:+68589063 None:intergenic
AGCACGAAAAGAGGGAGGTG+AGG 0.582858 4_4:+68587881 None:intergenic
CCCTGTAGTGATGATTCAAA+TGG 0.586439 4_4:+68586244 None:intergenic
TCATCAACCTATTATGGGCA+AGG 0.588235 4_4:-68588227 Msa0698310:CDS
AATAGCTCTTAGTTTGAACA+TGG 0.589170 4_4:+68587804 None:intergenic
GGCACAGGTAAGTGAGTGAG+TGG 0.590238 4_4:+68589078 None:intergenic
TAGAAAGGGGTATACCATGT+AGG 0.600427 4_4:+68587682 None:intergenic
AAAATGCCTCCAAACACAGC+AGG 0.605294 4_4:+68586841 None:intergenic
CACAGGTAAGTGAGTGAGTG+GGG 0.605325 4_4:+68589080 None:intergenic
GCACAGGTAAGTGAGTGAGT+GGG 0.606218 4_4:+68589079 None:intergenic
TGACACGAAAGGTCGAGAAG+CGG 0.609671 4_4:-68588876 Msa0698310:five_prime_UTR
GTAATCTAAATTAAGGAATG+GGG 0.610068 4_4:+68588777 None:intergenic
CAAGTCTTAAGCTATCCATA+GGG 0.618487 4_4:+68587735 None:intergenic
TGCCGAGTAGCACGAAAAGA+GGG 0.619206 4_4:+68587873 None:intergenic
ATAAACACGTTTGACACGAA+AGG 0.619757 4_4:-68588887 Msa0698310:five_prime_UTR
TATCCATAGGGTGCTTATGG+TGG 0.620677 4_4:+68587747 None:intergenic
TTAGCCTCCCTCTTTCAAAG+AGG 0.624702 4_4:-68588677 Msa0698310:five_prime_UTR
GTGAAGTGATGCCAAAGCCA+AGG 0.625285 4_4:+68586214 None:intergenic
CCAGAGAAAGGACTGTGCAG+AGG 0.629364 4_4:+68585929 None:intergenic
ACAGCAGGAGCAATTGAAGG+AGG 0.629758 4_4:+68586856 None:intergenic
CCATTTGAATCATCACTACA+GGG 0.637039 4_4:-68586244 Msa0698310:CDS
AAAGAGGGAGGTGAGGAACA+GGG 0.637398 4_4:+68587888 None:intergenic
GTTGGTTACCTGACAAACAA+GGG 0.639760 4_4:+68588492 None:intergenic
TTGGTTACCTGACAAACAAG+GGG 0.643258 4_4:+68588493 None:intergenic
CAGCAGGAGCAATTGAAGGA+GGG 0.648334 4_4:+68586857 None:intergenic
TACTGCTATGAAAGAAACGA+GGG 0.655830 4_4:+68588200 None:intergenic
TCTCCCTCTTTGAAAGAGGG+AGG 0.658477 4_4:+68588673 None:intergenic
TCTAAAGGAAGAAGGAATGG+TGG 0.659442 4_4:+68585893 None:intergenic
TACCATGTAGGCTATTAAAG+CGG 0.682242 4_4:+68587694 None:intergenic
AAAAGGAAAGAACATTTGGG+GGG 0.683500 4_4:-68588948 Msa0698310:five_prime_UTR
CGAGTAGCACGAAAAGAGGG+AGG 0.696341 4_4:+68587876 None:intergenic
ATACTGCTATGAAAGAAACG+AGG 0.714480 4_4:+68588199 None:intergenic
AGGGAATACAAGCTTCACTG+AGG 0.754775 4_4:+68586876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATGTAATCTAAATTA+AGG + chr4_4:68586230-68586249 None:intergenic 10.0%
!! TTTATATAAACTCTTTATTA+TGG - chr4_4:68587454-68587473 Msa0698310:intron 10.0%
!! AAAAAATAGAAGAAAAAACA+GGG - chr4_4:68586076-68586095 Msa0698310:three_prime_UTR 15.0%
!! TATATTTGAATTCTATTATG+AGG - chr4_4:68586166-68586185 Msa0698310:CDS 15.0%
!! AGAACAAAAAAAGAAAAATT+AGG + chr4_4:68586431-68586450 None:intergenic 15.0%
!!! ATAATCAATTTTAATGCATA+TGG + chr4_4:68586880-68586899 None:intergenic 15.0%
!!! TGCATTAAAATTGATTATTT+TGG - chr4_4:68586882-68586901 Msa0698310:CDS 15.0%
!!! TCAACTATTTTAAAGAAATT+TGG - chr4_4:68588514-68588533 Msa0698310:CDS 15.0%
!! ATCAAATTTGATACATATTA+TGG + chr4_4:68589029-68589048 None:intergenic 15.0%
!! AAAAATATGAGAAAGATCAT+TGG + chr4_4:68585943-68585962 None:intergenic 20.0%
!! GAAAAAATAGAAGAAAAAAC+AGG - chr4_4:68586075-68586094 Msa0698310:three_prime_UTR 20.0%
!! AATATACAAATACAGTATAC+AGG + chr4_4:68586152-68586171 None:intergenic 20.0%
!! TGTAATCTAAATTAAGGAAT+GGG + chr4_4:68586224-68586243 None:intergenic 20.0%
!! ATGTAATCTAAATTAAGGAA+TGG + chr4_4:68586225-68586244 None:intergenic 20.0%
!! AAAAAAAAAGTGAATTGAGT+TGG + chr4_4:68586526-68586545 None:intergenic 20.0%
!! ATGAAAATTTAATGTAGGTA+GGG + chr4_4:68586848-68586867 None:intergenic 20.0%
!! GAATGATGAAAATTTAATGT+AGG + chr4_4:68586853-68586872 None:intergenic 20.0%
!! ATTATTAAGATTTGTGTTGT+TGG - chr4_4:68586992-68587011 Msa0698310:intron 20.0%
!! ATTTATAACTCTTTCAGATT+GGG - chr4_4:68587083-68587102 Msa0698310:intron 20.0%
!! ACAAAAGAAAAATGTAGAAA+GGG + chr4_4:68587332-68587351 None:intergenic 20.0%
!! AACAAAAGAAAAATGTAGAA+AGG + chr4_4:68587333-68587352 None:intergenic 20.0%
!! AAATCATGCATATAATGAAA+AGG + chr4_4:68587370-68587389 None:intergenic 20.0%
!!! ATTTCATTGTTTAGTTTTGA+AGG - chr4_4:68587599-68587618 Msa0698310:intron 20.0%
!! ATAATATTTGACATACTTGT+AGG - chr4_4:68587738-68587757 Msa0698310:CDS 20.0%
!! TAATATTTGACATACTTGTA+GGG - chr4_4:68587739-68587758 Msa0698310:CDS 20.0%
! AGAGACTATACAAAAAAAAG+AGG - chr4_4:68586014-68586033 Msa0698310:three_prime_UTR 25.0%
! CAAAAAAGGAAAGAACATTT+GGG - chr4_4:68586046-68586065 Msa0698310:three_prime_UTR 25.0%
! AAAAAAGGAAAGAACATTTG+GGG - chr4_4:68586047-68586066 Msa0698310:three_prime_UTR 25.0%
! GTAATCTAAATTAAGGAATG+GGG + chr4_4:68586223-68586242 None:intergenic 25.0%
! TAAAATCTCAACAACTGAAT+TGG + chr4_4:68586303-68586322 None:intergenic 25.0%
! GATGAGATAATTAAAATGTC+TGG + chr4_4:68586616-68586635 None:intergenic 25.0%
! AAGTAAATTCTTAAGTACTC+TGG + chr4_4:68586680-68586699 None:intergenic 25.0%
! TTGGTGAAGATTATGATAAA+TGG - chr4_4:68586745-68586764 Msa0698310:intron 25.0%
! TGGTGAAGATTATGATAAAT+GGG - chr4_4:68586746-68586765 Msa0698310:intron 25.0%
! GATGAAAATTTAATGTAGGT+AGG + chr4_4:68586849-68586868 None:intergenic 25.0%
! TAAATGAAACAAGATGCAAT+TGG + chr4_4:68587068-68587087 None:intergenic 25.0%
! CATTTATAACTCTTTCAGAT+TGG - chr4_4:68587082-68587101 Msa0698310:intron 25.0%
! TTTGAACATGGAAAAGAAAA+CGG + chr4_4:68587184-68587203 None:intergenic 25.0%
! CAAAAGAAAAATGTAGAAAG+GGG + chr4_4:68587331-68587350 None:intergenic 25.0%
! TTTCTACTTGAAATGATTAG+AGG + chr4_4:68587483-68587502 None:intergenic 25.0%
!!! TTTAGTTTTGAAGGAATGAA+TGG - chr4_4:68587608-68587627 Msa0698310:intron 25.0%
!!! TTAGTTTTGAAGGAATGAAT+GGG - chr4_4:68587609-68587628 Msa0698310:intron 25.0%
!! GTATCTAAAACAAACTTCTA+AGG + chr4_4:68587648-68587667 None:intergenic 25.0%
! TATACCAATTCAATTGATGT+TGG - chr4_4:68587669-68587688 Msa0698310:intron 25.0%
!!! TTAAGTACTTTGAAATCTTG+AGG - chr4_4:68587708-68587727 Msa0698310:CDS 25.0%
! AATATTTGACATACTTGTAG+GGG - chr4_4:68587740-68587759 Msa0698310:CDS 25.0%
!! ATTTTGCAAAAGTAACACAT+TGG + chr4_4:68588317-68588336 None:intergenic 25.0%
! TGTTGTTACTGAAGAAAATA+AGG + chr4_4:68588541-68588560 None:intergenic 25.0%
!! AATTGTTACTTATCTACGTT+TGG + chr4_4:68588860-68588879 None:intergenic 25.0%
! TGGTTATTTATAGCAAATTG+AGG - chr4_4:68588899-68588918 Msa0698310:five_prime_UTR 25.0%
!!! AGAGAATTCTTCACTTTTTT+TGG + chr4_4:68588933-68588952 None:intergenic 25.0%
!! TGGATTTTTACACAATGTTA+TGG + chr4_4:68589009-68589028 None:intergenic 25.0%
! TTTTTCCAGATCATCATGTT+CGG - chr4_4:68585962-68585981 Msa0698310:three_prime_UTR 30.0%
GCAAAAAAGGAAAGAACATT+TGG - chr4_4:68586045-68586064 Msa0698310:three_prime_UTR 30.0%
AAAAAGGAAAGAACATTTGG+GGG - chr4_4:68586048-68586067 Msa0698310:three_prime_UTR 30.0%
!! TTGTCTTTTTGTTTAACGTG+TGG - chr4_4:68586189-68586208 Msa0698310:CDS 30.0%
!! CTTATTTTTCAGTTGCAAGA+TGG - chr4_4:68586449-68586468 Msa0698310:intron 30.0%
TTTCTTTCATAGCAGTATCT+TGG - chr4_4:68586803-68586822 Msa0698310:CDS 30.0%
AATTTAATGTAGGTAGGGTA+GGG + chr4_4:68586843-68586862 None:intergenic 30.0%
AAATTTAATGTAGGTAGGGT+AGG + chr4_4:68586844-68586863 None:intergenic 30.0%
AAAACGGTGCAATAAGTATT+CGG + chr4_4:68587168-68587187 None:intergenic 30.0%
! AATAGCTCTTAGTTTGAACA+TGG + chr4_4:68587196-68587215 None:intergenic 30.0%
ATGTTCAAACTAAGAGCTAT+TGG - chr4_4:68587195-68587214 Msa0698310:intron 30.0%
TGTTCAAACTAAGAGCTATT+GGG - chr4_4:68587196-68587215 Msa0698310:intron 30.0%
TATGTCACAGTTATCTTTAG+AGG + chr4_4:68587398-68587417 None:intergenic 30.0%
CATCATAAGATTCATAACTG+TGG - chr4_4:68587551-68587570 Msa0698310:intron 30.0%
GATACCAACATCAATTGAAT+TGG + chr4_4:68587676-68587695 None:intergenic 30.0%
! TGTGTCTTTCTTGTTTGATA+AGG + chr4_4:68587866-68587885 None:intergenic 30.0%
TGTCAATAGTTCTTCTATAG+AGG + chr4_4:68588024-68588043 None:intergenic 30.0%
ACACATTATTACTTGCATCA+TGG - chr4_4:68588204-68588223 Msa0698310:CDS 30.0%
!!! TTTTTTGGCACATTACTCTT+TGG + chr4_4:68588234-68588253 None:intergenic 30.0%
TAATGTGCCAAAAAATCTCA+AGG - chr4_4:68588239-68588258 Msa0698310:CDS 30.0%
!!! GCTTATACCTTGAGATTTTT+TGG + chr4_4:68588249-68588268 None:intergenic 30.0%
TGGTAAGGTAAACTATACAA+TGG + chr4_4:68588358-68588377 None:intergenic 30.0%
TTTCGATCAACTAATATGTC+TGG + chr4_4:68588384-68588403 None:intergenic 30.0%
CATATTAGTTGATCGAAAAC+TGG - chr4_4:68588386-68588405 Msa0698310:intron 30.0%
TTCCGTTTCATTTATCCATA+TGG - chr4_4:68588449-68588468 Msa0698310:intron 30.0%
TCCGTTTCATTTATCCATAT+GGG - chr4_4:68588450-68588469 Msa0698310:intron 30.0%
ACCCATATGGATAAATGAAA+CGG + chr4_4:68588454-68588473 None:intergenic 30.0%
CTGTTTATCTTTCACCAAAA+AGG + chr4_4:68588568-68588587 None:intergenic 30.0%
ATGTCATGTTCGAAAAAGAA+AGG + chr4_4:68588618-68588637 None:intergenic 30.0%
AATGGTACTTCTGCAAAATA+AGG + chr4_4:68588738-68588757 None:intergenic 30.0%
TGAGATTTGCATTTGTTCAT+TGG - chr4_4:68588879-68588898 Msa0698310:five_prime_UTR 30.0%
! TCTAATTTTCTTTGCTCCTT+TGG - chr4_4:68588962-68588981 Msa0698310:five_prime_UTR 30.0%
TTAAGCAATCTAAAGGAAGA+AGG + chr4_4:68589115-68589134 None:intergenic 30.0%
AGAGGACTAAAGAGCAAAAA+AGG - chr4_4:68586032-68586051 Msa0698310:three_prime_UTR 35.0%
AAAAGGAAAGAACATTTGGG+GGG - chr4_4:68586049-68586068 Msa0698310:three_prime_UTR 35.0%
GTGTCAAACGTGTTTATTAC+AGG + chr4_4:68586107-68586126 None:intergenic 35.0%
ATAAACACGTTTGACACGAA+AGG - chr4_4:68586110-68586129 Msa0698310:three_prime_UTR 35.0%
!! TTCTTAAGTACTCTGGAGTT+TGG + chr4_4:68586673-68586692 None:intergenic 35.0%
AATTGACAACACTATGTGAC+AGG - chr4_4:68586713-68586732 Msa0698310:intron 35.0%
ATGGGTTCATCAACCTATTA+TGG - chr4_4:68586764-68586783 Msa0698310:CDS 35.0%
TGGGTTCATCAACCTATTAT+GGG - chr4_4:68586765-68586784 Msa0698310:CDS 35.0%
TACTGCTATGAAAGAAACGA+GGG + chr4_4:68586800-68586819 None:intergenic 35.0%
ATACTGCTATGAAAGAAACG+AGG + chr4_4:68586801-68586820 None:intergenic 35.0%
!! TCTCTGGAATTGTGTTTAGA+GGG - chr4_4:68586928-68586947 Msa0698310:intron 35.0%
! GAATTGTGTTTAGAGGGTTT+TGG - chr4_4:68586934-68586953 Msa0698310:intron 35.0%
! AAGATTTGTGTTGTTGGAAC+AGG - chr4_4:68586998-68587017 Msa0698310:intron 35.0%
GTGGGTGATTCCAATTATTT+GGG - chr4_4:68587040-68587059 Msa0698310:intron 35.0%
GCAATTGGAACCCAAATAAT+TGG + chr4_4:68587053-68587072 None:intergenic 35.0%
GTTCAAACTAAGAGCTATTG+GGG - chr4_4:68587197-68587216 Msa0698310:intron 35.0%
ACATTGCATTATCTTCTCCA+TGG - chr4_4:68587224-68587243 Msa0698310:intron 35.0%
CAAGTCTTAAGCTATCCATA+GGG + chr4_4:68587265-68587284 None:intergenic 35.0%
ACAAGTCTTAAGCTATCCAT+AGG + chr4_4:68587266-68587285 None:intergenic 35.0%
TACCATGTAGGCTATTAAAG+CGG + chr4_4:68587306-68587325 None:intergenic 35.0%
TAACTGTGACATAGTTTCTC+TGG - chr4_4:68587405-68587424 Msa0698310:intron 35.0%
! TGTGGTGTTAGTGTAAATGA+TGG - chr4_4:68587569-68587588 Msa0698310:intron 35.0%
TTTGAAGGAATGAATGGGAA+TGG - chr4_4:68587614-68587633 Msa0698310:intron 35.0%
TTGAAGGAATGAATGGGAAT+GGG - chr4_4:68587615-68587634 Msa0698310:intron 35.0%
TGATGTTGGTATCAGAAGTA+CGG - chr4_4:68587683-68587702 Msa0698310:intron 35.0%
AAGTCTGTTAGTATTAGACG+AGG - chr4_4:68587769-68587788 Msa0698310:CDS 35.0%
ACGAGGAAGAAAGTAATGTT+AGG - chr4_4:68587786-68587805 Msa0698310:CDS 35.0%
CGAGGAAGAAAGTAATGTTA+GGG - chr4_4:68587787-68587806 Msa0698310:CDS 35.0%
TGTATGGTAGCAATACATGA+AGG + chr4_4:68587834-68587853 None:intergenic 35.0%
GATAAGGCTTATGAGTTGTA+TGG + chr4_4:68587850-68587869 None:intergenic 35.0%
!! GTATTTCTGGCTAGTTGAAA+CGG + chr4_4:68587908-68587927 None:intergenic 35.0%
CGAAACAAGACATGTATTTC+TGG + chr4_4:68587921-68587940 None:intergenic 35.0%
GCAGTAAATGAGTAGTACAT+AGG - chr4_4:68587994-68588013 Msa0698310:intron 35.0%
CACTTTCCCTTCTTAAAATG+TGG - chr4_4:68588268-68588287 Msa0698310:intron 35.0%
!! TGTCGTCCACATTTTAAGAA+GGG + chr4_4:68588277-68588296 None:intergenic 35.0%
!! TTGTCGTCCACATTTTAAGA+AGG + chr4_4:68588278-68588297 None:intergenic 35.0%
AAAAGTAACACATTGGAGCA+AGG + chr4_4:68588310-68588329 None:intergenic 35.0%
!! TAATATGTCTGGCTTTGGTA+AGG + chr4_4:68588373-68588392 None:intergenic 35.0%
! TCAACTAATATGTCTGGCTT+TGG + chr4_4:68588378-68588397 None:intergenic 35.0%
AATGTCACAGTAGTAACTAG+TGG + chr4_4:68588426-68588445 None:intergenic 35.0%
CATATGGGTCTCTTAAACTT+GGG - chr4_4:68588465-68588484 Msa0698310:intron 35.0%
! AGTAACAACAGTGACCTTTT+TGG - chr4_4:68588551-68588570 Msa0698310:intron 35.0%
AGCTCCTACTAAGAATATGA+TGG - chr4_4:68588586-68588605 Msa0698310:intron 35.0%
TGCACCATCATATTCTTAGT+AGG + chr4_4:68588593-68588612 None:intergenic 35.0%
ACTTGTCAGTTGTCTTTCTA+AGG + chr4_4:68588704-68588723 None:intergenic 35.0%
GTACTTCTGCAAAATAAGGA+AGG + chr4_4:68588734-68588753 None:intergenic 35.0%
ACCATTTGAATCATCACTAC+AGG - chr4_4:68588752-68588771 Msa0698310:five_prime_UTR 35.0%
CCATTTGAATCATCACTACA+GGG - chr4_4:68588753-68588772 Msa0698310:five_prime_UTR 35.0%
!! ATGGGATAAGGTTTTTGGAA+CGG - chr4_4:68588810-68588829 Msa0698310:five_prime_UTR 35.0%
TAACCCTCAAAAAACACCAA+AGG + chr4_4:68588981-68589000 None:intergenic 35.0%
CAATCTAAAGGAAGAAGGAA+TGG + chr4_4:68589110-68589129 None:intergenic 35.0%
ATGAGAAAGATCATTGGCAC+AGG + chr4_4:68585937-68585956 None:intergenic 40.0%
! AAGTCTCCCTCTTTGAAAGA+GGG + chr4_4:68586330-68586349 None:intergenic 40.0%
! TAAGTCTCCCTCTTTGAAAG+AGG + chr4_4:68586331-68586350 None:intergenic 40.0%
AAAAGAAGCAGAAGCAGATC+TGG + chr4_4:68586393-68586412 None:intergenic 40.0%
! GAATAAACCCCTTGTTTGTC+AGG - chr4_4:68586497-68586516 Msa0698310:intron 40.0%
TTGGTTACCTGACAAACAAG+GGG + chr4_4:68586507-68586526 None:intergenic 40.0%
GTTGGTTACCTGACAAACAA+GGG + chr4_4:68586508-68586527 None:intergenic 40.0%
AGTTGGTTACCTGACAAACA+AGG + chr4_4:68586509-68586528 None:intergenic 40.0%
TAATCCTATTCGAGTCGTGT+CGG - chr4_4:68586564-68586583 Msa0698310:intron 40.0%
! TCTGGAGTTTGGTTGAGTTT+CGG + chr4_4:68586662-68586681 None:intergenic 40.0%
TCATCAACCTATTATGGGCA+AGG - chr4_4:68586770-68586789 Msa0698310:CDS 40.0%
CTTTCATAGCAGTATCTTGG+AGG - chr4_4:68586806-68586825 Msa0698310:CDS 40.0%
!! CGACTCAAAAGATTAGTCTC+TGG - chr4_4:68586912-68586931 Msa0698310:intron 40.0%
!! GTCTCTGGAATTGTGTTTAG+AGG - chr4_4:68586927-68586946 Msa0698310:intron 40.0%
GGTGGGTGATTCCAATTATT+TGG - chr4_4:68587039-68587058 Msa0698310:intron 40.0%
! TGCTACTCGGCATTTTTGTA+TGG - chr4_4:68587135-68587154 Msa0698310:intron 40.0%
! TAGAAAGGGGTATACCATGT+AGG + chr4_4:68587318-68587337 None:intergenic 40.0%
AATGTTAGGGTCAAATGCAG+AGG - chr4_4:68587800-68587819 Msa0698310:CDS 40.0%
!!! TGTGTTTGGAGGCATTTTGT+TGG - chr4_4:68588161-68588180 Msa0698310:intron 40.0%
!!! GTGTTTGGAGGCATTTTGTT+GGG - chr4_4:68588162-68588181 Msa0698310:intron 40.0%
CCAAGTTTAAGAGACCCATA+TGG + chr4_4:68588467-68588486 None:intergenic 40.0%
CCATATGGGTCTCTTAAACT+TGG - chr4_4:68588464-68588483 Msa0698310:intron 40.0%
CCCTGTAGTGATGATTCAAA+TGG + chr4_4:68588756-68588775 None:intergenic 40.0%
CCAAAAACCTTATCCCATAG+TGG + chr4_4:68588808-68588827 None:intergenic 40.0%
! CCACTATGGGATAAGGTTTT+TGG - chr4_4:68588805-68588824 Msa0698310:five_prime_UTR 40.0%
!!! TGCTCCTTTGGTGTTTTTTG+AGG - chr4_4:68588974-68588993 Msa0698310:five_prime_UTR 40.0%
!!! GCTCCTTTGGTGTTTTTTGA+GGG - chr4_4:68588975-68588994 Msa0698310:five_prime_UTR 40.0%
AGGAAGAAGTTACCAGAGAA+AGG + chr4_4:68589083-68589102 None:intergenic 40.0%
TCTAAAGGAAGAAGGAATGG+TGG + chr4_4:68589107-68589126 None:intergenic 40.0%
GGACGACACAGTGGTAAATT+GGG + chr4_4:68585887-68585906 None:intergenic 45.0%
! AGTGAGTGGGGTATGTTTTG+GGG + chr4_4:68585908-68585927 None:intergenic 45.0%
! GAGTGAGTGGGGTATGTTTT+GGG + chr4_4:68585909-68585928 None:intergenic 45.0%
! TGAGTGAGTGGGGTATGTTT+TGG + chr4_4:68585910-68585929 None:intergenic 45.0%
CTGTTCCGAACATGATGATC+TGG + chr4_4:68585970-68585989 None:intergenic 45.0%
TTAGCCTCCCTCTTTCAAAG+AGG - chr4_4:68586320-68586339 Msa0698310:intron 45.0%
TAGCCTCCCTCTTTCAAAGA+GGG - chr4_4:68586321-68586340 Msa0698310:intron 45.0%
CTTCTCTTCTCAGATCTCCT+CGG - chr4_4:68586354-68586373 Msa0698310:intron 45.0%
CTGGAAAATGAAACGAACCG+AGG + chr4_4:68586374-68586393 None:intergenic 45.0%
GACAACACTATGTGACAGGT+TGG - chr4_4:68586717-68586736 Msa0698310:intron 45.0%
!! ATGTGACAGGTTGGTCATCT+TGG - chr4_4:68586726-68586745 Msa0698310:intron 45.0%
CAACCTATTATGGGCAAGGA+AGG - chr4_4:68586774-68586793 Msa0698310:CDS 45.0%
AATGTAGGTAGGGTAGGGTA+GGG + chr4_4:68586838-68586857 None:intergenic 45.0%
TAATGTAGGTAGGGTAGGGT+AGG + chr4_4:68586839-68586858 None:intergenic 45.0%
! TATCCATAGGGTGCTTATGG+TGG + chr4_4:68587253-68587272 None:intergenic 45.0%
! AGCTATCCATAGGGTGCTTA+TGG + chr4_4:68587256-68587275 None:intergenic 45.0%
!! AGACTTGTTTTTCCACCAGC+TGG - chr4_4:68587278-68587297 Msa0698310:intron 45.0%
! AGCCGCTTTAATAGCCTACA+TGG - chr4_4:68587301-68587320 Msa0698310:intron 45.0%
TAGGGTCAAATGCAGAGGTT+AGG - chr4_4:68587805-68587824 Msa0698310:CDS 45.0%
TTGTTTGCAACGTCCAGATC+TGG - chr4_4:68588097-68588116 Msa0698310:intron 45.0%
AGGGAATACAAGCTTCACTG+AGG + chr4_4:68588124-68588143 None:intergenic 45.0%
AACACAGCAGGAGCAATTGA+AGG + chr4_4:68588147-68588166 None:intergenic 45.0%
TCAATTGCTCCTGCTGTGTT+TGG - chr4_4:68588147-68588166 Msa0698310:intron 45.0%
AAAATGCCTCCAAACACAGC+AGG + chr4_4:68588159-68588178 None:intergenic 45.0%
TTGGCATCACTTCACCACTA+TGG - chr4_4:68588791-68588810 Msa0698310:five_prime_UTR 45.0%
TGGCATCACTTCACCACTAT+GGG - chr4_4:68588792-68588811 Msa0698310:five_prime_UTR 45.0%
CACTTCACCACTATGGGATA+AGG - chr4_4:68588798-68588817 Msa0698310:five_prime_UTR 45.0%
!! TTGGCATCCACTTTTGATGG+AGG + chr4_4:68588841-68588860 None:intergenic 45.0%
!! CGTTTGGCATCCACTTTTGA+TGG + chr4_4:68588844-68588863 None:intergenic 45.0%
AAGGAAGAAGGAATGGTGGT+AGG + chr4_4:68589103-68589122 None:intergenic 45.0%
GGGACGACACAGTGGTAAAT+TGG + chr4_4:68585888-68585907 None:intergenic 50.0%
!!! ATGTTTTGGGGACGACACAG+TGG + chr4_4:68585896-68585915 None:intergenic 50.0%
CACAGGTAAGTGAGTGAGTG+GGG + chr4_4:68585920-68585939 None:intergenic 50.0%
GCACAGGTAAGTGAGTGAGT+GGG + chr4_4:68585921-68585940 None:intergenic 50.0%
TGACACGAAAGGTCGAGAAG+CGG - chr4_4:68586121-68586140 Msa0698310:three_prime_UTR 50.0%
! TCTCCCTCTTTGAAAGAGGG+AGG + chr4_4:68586327-68586346 None:intergenic 50.0%
! AGGTTTTCACGCGAACTGCT+TGG - chr4_4:68587018-68587037 Msa0698310:intron 50.0%
! TTTTCACGCGAACTGCTTGG+TGG - chr4_4:68587021-68587040 Msa0698310:intron 50.0%
TTTCACGCGAACTGCTTGGT+GGG - chr4_4:68587022-68587041 Msa0698310:intron 50.0%
AAAGAGGGAGGTGAGGAACA+GGG + chr4_4:68587112-68587131 None:intergenic 50.0%
AAAAGAGGGAGGTGAGGAAC+AGG + chr4_4:68587113-68587132 None:intergenic 50.0%
!! CTCCCTCTTTTCGTGCTACT+CGG - chr4_4:68587122-68587141 Msa0698310:intron 50.0%
TGCCGAGTAGCACGAAAAGA+GGG + chr4_4:68587127-68587146 None:intergenic 50.0%
ATGCCGAGTAGCACGAAAAG+AGG + chr4_4:68587128-68587147 None:intergenic 50.0%
TTGCCACCATAAGCACCCTA+TGG - chr4_4:68587247-68587266 Msa0698310:intron 50.0%
CAGCAGGAGCAATTGAAGGA+GGG + chr4_4:68588143-68588162 None:intergenic 50.0%
ACAGCAGGAGCAATTGAAGG+AGG + chr4_4:68588144-68588163 None:intergenic 50.0%
ATTGCTCCTGCTGTGTTTGG+AGG - chr4_4:68588150-68588169 Msa0698310:intron 50.0%
GTGAAGTGATGCCAAAGCCA+AGG + chr4_4:68588786-68588805 None:intergenic 50.0%
GGTTCCTCCTCCATCAAAAG+TGG - chr4_4:68588831-68588850 Msa0698310:five_prime_UTR 50.0%
!! GCATCCACTTTTGATGGAGG+AGG + chr4_4:68588838-68588857 None:intergenic 50.0%
GGCACAGGTAAGTGAGTGAG+TGG + chr4_4:68585922-68585941 None:intergenic 55.0%
GTGTCCGACACGACTCGAAT+AGG + chr4_4:68586571-68586590 None:intergenic 55.0%
GGGCCTTCCTTGCCCATAAT+AGG + chr4_4:68586780-68586799 None:intergenic 55.0%
TAGGTAGGGTAGGGTAGGGT+AGG + chr4_4:68586834-68586853 None:intergenic 55.0%
AGCACGAAAAGAGGGAGGTG+AGG + chr4_4:68587119-68587138 None:intergenic 55.0%
CGAGTAGCACGAAAAGAGGG+AGG + chr4_4:68587124-68587143 None:intergenic 55.0%
GGTGCTTATGGTGGCAACCA+TGG + chr4_4:68587244-68587263 None:intergenic 55.0%
ATTAAAGCGGCTCCAGCTGG+TGG + chr4_4:68587293-68587312 None:intergenic 55.0%
GCTATTAAAGCGGCTCCAGC+TGG + chr4_4:68587296-68587315 None:intergenic 55.0%
GCTTCACTGAGGACCAGATC+TGG + chr4_4:68588113-68588132 None:intergenic 55.0%
TGATGCCAAAGCCAAGGCTC+TGG + chr4_4:68588780-68588799 None:intergenic 55.0%
CCAGAGAAAGGACTGTGCAG+AGG + chr4_4:68589071-68589090 None:intergenic 55.0%
CCTCTGCACAGTCCTTTCTC+TGG - chr4_4:68589068-68589087 Msa0698310:five_prime_UTR 55.0%
CACTACAGGGTCCAGAGCCT+TGG - chr4_4:68588766-68588785 Msa0698310:five_prime_UTR 60.0%
AGGGTCCAGAGCCTTGGCTT+TGG - chr4_4:68588772-68588791 Msa0698310:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 68585880 68589139 68585880 ID=Msa0698310;Name=Msa0698310
chr4_4 mRNA 68585880 68589139 68585880 ID=Msa0698310-mRNA-1;Parent=Msa0698310;Name=Msa0698310-mRNA-1;_AED=0.11;_eAED=0.11;_QI=503|1|1|1|0.8|0.83|6|269|238
chr4_4 exon 68585880 68586271 68585880 ID=Msa0698310-mRNA-1:exon:20394;Parent=Msa0698310-mRNA-1
chr4_4 exon 68586759 68586905 68586759 ID=Msa0698310-mRNA-1:exon:20393;Parent=Msa0698310-mRNA-1
chr4_4 exon 68587697 68587999 68587697 ID=Msa0698310-mRNA-1:exon:20392;Parent=Msa0698310-mRNA-1
chr4_4 exon 68588192 68588284 68588192 ID=Msa0698310-mRNA-1:exon:20391;Parent=Msa0698310-mRNA-1
chr4_4 exon 68588501 68588558 68588501 ID=Msa0698310-mRNA-1:exon:20390;Parent=Msa0698310-mRNA-1
chr4_4 exon 68588644 68589139 68588644 ID=Msa0698310-mRNA-1:exon:20389;Parent=Msa0698310-mRNA-1
chr4_4 five_prime_UTR 68588644 68589139 68588644 ID=Msa0698310-mRNA-1:five_prime_utr;Parent=Msa0698310-mRNA-1
chr4_4 five_prime_UTR 68588552 68588558 68588552 ID=Msa0698310-mRNA-1:five_prime_utr;Parent=Msa0698310-mRNA-1
chr4_4 CDS 68588501 68588551 68588501 ID=Msa0698310-mRNA-1:cds;Parent=Msa0698310-mRNA-1
chr4_4 CDS 68588192 68588284 68588192 ID=Msa0698310-mRNA-1:cds;Parent=Msa0698310-mRNA-1
chr4_4 CDS 68587697 68587999 68587697 ID=Msa0698310-mRNA-1:cds;Parent=Msa0698310-mRNA-1
chr4_4 CDS 68586759 68586905 68586759 ID=Msa0698310-mRNA-1:cds;Parent=Msa0698310-mRNA-1
chr4_4 CDS 68586149 68586271 68586149 ID=Msa0698310-mRNA-1:cds;Parent=Msa0698310-mRNA-1
chr4_4 three_prime_UTR 68585880 68586148 68585880 ID=Msa0698310-mRNA-1:three_prime_utr;Parent=Msa0698310-mRNA-1
Gene Sequence

>Msa0698310

ATGGTTGCAAACAAATTTGTTGTTGATTTGAATAAACCCCTTGTTTGTCAGGTTGGTCATCTTGGTGAAGATTATGATAAATGGGTTCATCAACCTATTATGGGCAAGGAAGGCCCTCGTTTCTTTCATAGCAGTATCTTGGAGGTTTTCACGCGAACTGCTTGGTGGGTGATTCCAATTATTTGGGTTCCAATTGCATCTTGTTTCATTTATAACTCTTTCAGATTGGGACTCCCTGTTCCTCACCTCCCTCTTTTCGTGCTACTCGGCATTTTTGTATGGACATTGACCGAATACTTATTGCACCGTTTTCTTTTCCATGTTCAAACTAAGAGCTATTGGGGTAATACATTGCATTATCTTCTCCATGGTTGCCACCATAAGCACCCTATGGATAGCTTAAGACTTGTTTTTCCACCAGCTGGAGCCGCTTTAATAGCCTACATGATCTGGTCCTCAGTGAAGCTTGTATTCCCTCCTTCAATTGCTCCTGCTGTGTTTGGAGGCATTTTGTTGGGCTATGTGATGTATGATTGCACACATTATTACTTGCATCATGGTCAACCAAAGAGTAATGTGCCAAAAAATCTCAAGAAGTACCATTTGAATCATCACTACAGGGTCCAGAGCCTTGGCTTTGGCATCACTTCACCACTATGGGATAAGGTTTTTGGAACGGTTCCTCCTCCATCAAAAGTGGATGCCAAACGTAGATAA
Protein sequence

>Msa0698310

MVANKFVVDLNKPLVCQVGHLGEDYDKWVHQPIMGKEGPRFFHSSILEVFTRTAWWVIPIIWVPIASCFIYNSFRLGLPVPHLPLFVLLGIFVWTLTEYLLHRFLFHVQTKSYWGNTLHYLLHGCHHKHPMDSLRLVFPPAGAALIAYMIWSSVKLVFPPSIAPAVFGGILLGYVMYDCTHYYLHHGQPKSNVPKNLKKYHLNHHYRVQSLGFGITSPLWDKVFGTVPPPSKVDAKRR*