AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0615270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0615270 MtrunA17_Chr4g0051591 97.899 238 5 0 1 238 1 238 3.22e-175 481
Msa0615270 MtrunA17_Chr2g0292941 68.776 237 73 1 1 237 1 236 1.05e-124 353
Msa0615270 MtrunA17_Chr4g0051581 80.952 147 28 0 1 147 1 147 8.96e-77 229
Msa0615270 MtrunA17_Chr4g0051571 78.161 87 19 0 151 237 10 96 2.66e-45 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0615270 AT2G34770.1 63.158 228 84 0 1 228 1 228 2.22e-108 312
Msa0615270 AT4G20870.2 61.603 237 90 1 1 237 1 236 1.07e-106 308
Msa0615270 AT4G20870.1 61.603 237 90 1 1 237 1 236 1.07e-106 308

Find 104 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGGTGATTCCAATTATT+TGG 0.142082 4_2:+64724714 Msa0615270:CDS
ATCAAATTTGATACATATTA+TGG 0.180771 4_2:-64726701 None:intergenic
ATGGGTTCATCAACCTATTA+TGG 0.183910 4_2:+64724439 Msa0615270:CDS
ATTATATGTAATCTAAATTA+AGG 0.210656 4_2:-64723892 None:intergenic
GGGACGACACAGTGGTAAAT+TGG 0.215450 4_2:-64723550 None:intergenic
GCAATTGGAACCCAAATAAT+TGG 0.230184 4_2:-64724725 None:intergenic
TTGGTGAAGATTATGATAAA+TGG 0.262773 4_2:+64724420 Msa0615270:CDS
AAAAGAGGGAGGTGAGGAAC+AGG 0.286347 4_2:-64724785 None:intergenic
GTGTCAAACGTGTTTATTAC+AGG 0.288300 4_2:-64723769 None:intergenic
TCAATTGCTCCTGCTGTGTT+TGG 0.295260 4_2:+64725822 Msa0615270:CDS
GTGGGTGATTCCAATTATTT+GGG 0.302503 4_2:+64724715 Msa0615270:CDS
TTGGCATCACTTCACCACTA+TGG 0.307245 4_2:+64726466 Msa0615270:CDS
TGAGATTTGCATTTGTTCAT+TGG 0.329247 4_2:+64726554 Msa0615270:three_prime_UTR
GGGCCTTCCTTGCCCATAAT+AGG 0.340890 4_2:-64724452 None:intergenic
ATGTTCAAACTAAGAGCTAT+TGG 0.342050 4_2:+64724870 Msa0615270:CDS
AGCTATCCATAGGGTGCTTA+TGG 0.348531 4_2:-64724928 None:intergenic
GAATAAACCCCTTGTTTGTC+AGG 0.349453 4_2:+64724169 Msa0615270:CDS
TTTCTTTCATAGCAGTATCT+TGG 0.357754 4_2:+64724478 Msa0615270:CDS
TATATTTGAATTCTATTATG+AGG 0.359179 4_2:+64723831 Msa0615270:five_prime_UTR
TGTTCAAACTAAGAGCTATT+GGG 0.361825 4_2:+64724871 Msa0615270:CDS
AATTGTTACTTATCTACGTT+TGG 0.364138 4_2:-64726532 None:intergenic
TGAGTGAGTGGGGTATGTTT+TGG 0.371283 4_2:-64723572 None:intergenic
CATTTATAACTCTTTCAGAT+TGG 0.376626 4_2:+64724757 Msa0615270:CDS
TGTAATCTAAATTAAGGAAT+GGG 0.377357 4_2:-64723886 None:intergenic
TAAAATCTCAACAACTGAAT+TGG 0.379417 4_2:-64723972 None:intergenic
ATTTATAACTCTTTCAGATT+GGG 0.403789 4_2:+64724758 Msa0615270:CDS
AGGGTCCAGAGCCTTGGCTT+TGG 0.410334 4_2:+64726447 Msa0615270:CDS
CACTTCACCACTATGGGATA+AGG 0.426484 4_2:+64726473 Msa0615270:CDS
TGGGTTCATCAACCTATTAT+GGG 0.432385 4_2:+64724440 Msa0615270:CDS
AAAACGGTGCAATAAGTATT+CGG 0.433689 4_2:-64724840 None:intergenic
TGGTGAAGATTATGATAAAT+GGG 0.434108 4_2:+64724421 Msa0615270:CDS
ATTGCTCCTGCTGTGTTTGG+AGG 0.436809 4_2:+64725825 Msa0615270:CDS
ACCATTTGAATCATCACTAC+AGG 0.439539 4_2:+64726427 Msa0615270:CDS
TGATGCCAAAGCCAAGGCTC+TGG 0.469577 4_2:-64726452 None:intergenic
TTTGAACATGGAAAAGAAAA+CGG 0.472915 4_2:-64724856 None:intergenic
CTTTCATAGCAGTATCTTGG+AGG 0.476416 4_2:+64724481 Msa0615270:CDS
CACTACAGGGTCCAGAGCCT+TGG 0.478105 4_2:+64726441 Msa0615270:CDS
TGGCATCACTTCACCACTAT+GGG 0.480111 4_2:+64726467 Msa0615270:CDS
ACACATTATTACTTGCATCA+TGG 0.488209 4_2:+64725879 Msa0615270:CDS
CTGTTCCGAACATGATGATC+TGG 0.488917 4_2:-64723632 None:intergenic
ATGTAATCTAAATTAAGGAA+TGG 0.489047 4_2:-64723887 None:intergenic
TGGTTATTTATAGCAAATTG+AGG 0.495536 4_2:+64726574 Msa0615270:three_prime_UTR
ACAAGTCTTAAGCTATCCAT+AGG 0.497021 4_2:-64724938 None:intergenic
TAAATGAAACAAGATGCAAT+TGG 0.498395 4_2:-64724740 None:intergenic
CCTCTGCACAGTCCTTTCTC+TGG 0.498899 4_2:+64726743 Msa0615270:three_prime_UTR
AATATACAAATACAGTATAC+AGG 0.499179 4_2:-64723814 None:intergenic
GGACGACACAGTGGTAAATT+GGG 0.507441 4_2:-64723549 None:intergenic
ACCTACATTAAGCAATCTAA+AGG 0.509852 4_2:-64726794 None:intergenic
ATGTGACAGGTTGGTCATCT+TGG 0.510797 4_2:+64724401 Msa0615270:intron
TCCTTTAGATTGCTTAATGT+AGG 0.511317 4_2:+64726793 Msa0615270:three_prime_UTR
AGGAAGAAGTTACCAGAGAA+AGG 0.512038 4_2:-64726755 None:intergenic
CAACCTATTATGGGCAAGGA+AGG 0.526322 4_2:+64724449 Msa0615270:CDS
TAAGTCTCCCTCTTTGAAAG+AGG 0.530065 4_2:-64724000 None:intergenic
AAGGAAGAAGGAATGGTGGT+AGG 0.532611 4_2:-64726775 None:intergenic
TTAAGCAATCTAAAGGAAGA+AGG 0.534545 4_2:-64726787 None:intergenic
AACACAGCAGGAGCAATTGA+AGG 0.534894 4_2:-64725819 None:intergenic
TAGCCTCCCTCTTTCAAAGA+GGG 0.541468 4_2:+64723993 Msa0615270:five_prime_UTR
GGTGCTTATGGTGGCAACCA+TGG 0.543946 4_2:-64724916 None:intergenic
GCTATTAAAGCGGCTCCAGC+TGG 0.544240 4_2:-64724968 None:intergenic
ACATTGCATTATCTTCTCCA+TGG 0.544817 4_2:+64724899 Msa0615270:CDS
ATGCCGAGTAGCACGAAAAG+AGG 0.548185 4_2:-64724800 None:intergenic
TTTCACGCGAACTGCTTGGT+GGG 0.549072 4_2:+64724697 Msa0615270:CDS
GGTTCCTCCTCCATCAAAAG+TGG 0.554501 4_2:+64726506 Msa0615270:CDS
ATTAAAGCGGCTCCAGCTGG+TGG 0.557678 4_2:-64724965 None:intergenic
CAATCTAAAGGAAGAAGGAA+TGG 0.559858 4_2:-64726782 None:intergenic
AGTTGGTTACCTGACAAACA+AGG 0.563299 4_2:-64724178 None:intergenic
CTTCTCTTCTCAGATCTCCT+CGG 0.565161 4_2:+64724026 Msa0615270:five_prime_UTR
TTGCCACCATAAGCACCCTA+TGG 0.565998 4_2:+64724922 Msa0615270:CDS
AAGTCTCCCTCTTTGAAAGA+GGG 0.576055 4_2:-64723999 None:intergenic
AGCCGCTTTAATAGCCTACA+TGG 0.576385 4_2:+64724976 Msa0615270:CDS
GTTCAAACTAAGAGCTATTG+GGG 0.580121 4_2:+64724872 Msa0615270:CDS
ATGAGAAAGATCATTGGCAC+AGG 0.580902 4_2:-64723599 None:intergenic
AGCACGAAAAGAGGGAGGTG+AGG 0.582858 4_2:-64724791 None:intergenic
CCCTGTAGTGATGATTCAAA+TGG 0.586439 4_2:-64726428 None:intergenic
TCATCAACCTATTATGGGCA+AGG 0.588235 4_2:+64724445 Msa0615270:CDS
AATAGCTCTTAGTTTGAACA+TGG 0.589170 4_2:-64724868 None:intergenic
GGCACAGGTAAGTGAGTGAG+TGG 0.590238 4_2:-64723584 None:intergenic
TAGAAAGGGGTATACCATGT+AGG 0.600427 4_2:-64724990 None:intergenic
AAAATGCCTCCAAACACAGC+AGG 0.605294 4_2:-64725831 None:intergenic
CACAGGTAAGTGAGTGAGTG+GGG 0.605325 4_2:-64723582 None:intergenic
GCACAGGTAAGTGAGTGAGT+GGG 0.606218 4_2:-64723583 None:intergenic
TGACACGAAAGGTCGAGAAG+CGG 0.609671 4_2:+64723786 Msa0615270:five_prime_UTR
GTAATCTAAATTAAGGAATG+GGG 0.610068 4_2:-64723885 None:intergenic
CAAGTCTTAAGCTATCCATA+GGG 0.618487 4_2:-64724937 None:intergenic
TGCCGAGTAGCACGAAAAGA+GGG 0.619206 4_2:-64724799 None:intergenic
ATAAACACGTTTGACACGAA+AGG 0.619757 4_2:+64723775 Msa0615270:five_prime_UTR
TATCCATAGGGTGCTTATGG+TGG 0.620677 4_2:-64724925 None:intergenic
TTAGCCTCCCTCTTTCAAAG+AGG 0.624702 4_2:+64723992 Msa0615270:five_prime_UTR
GTGAAGTGATGCCAAAGCCA+AGG 0.625285 4_2:-64726458 None:intergenic
CCAGAGAAAGGACTGTGCAG+AGG 0.629364 4_2:-64726743 None:intergenic
ACAGCAGGAGCAATTGAAGG+AGG 0.629758 4_2:-64725816 None:intergenic
CCATTTGAATCATCACTACA+GGG 0.637039 4_2:+64726428 Msa0615270:CDS
AAAGAGGGAGGTGAGGAACA+GGG 0.637398 4_2:-64724784 None:intergenic
GTTGGTTACCTGACAAACAA+GGG 0.639760 4_2:-64724177 None:intergenic
TTGGTTACCTGACAAACAAG+GGG 0.643258 4_2:-64724176 None:intergenic
CAGCAGGAGCAATTGAAGGA+GGG 0.648334 4_2:-64725815 None:intergenic
TACTGCTATGAAAGAAACGA+GGG 0.655830 4_2:-64724472 None:intergenic
TCTCCCTCTTTGAAAGAGGG+AGG 0.658477 4_2:-64723996 None:intergenic
TCTAAAGGAAGAAGGAATGG+TGG 0.659442 4_2:-64726779 None:intergenic
TACCATGTAGGCTATTAAAG+CGG 0.682242 4_2:-64724978 None:intergenic
AAAAGGAAAGAACATTTGGG+GGG 0.683500 4_2:+64723714 Msa0615270:five_prime_UTR
CGAGTAGCACGAAAAGAGGG+AGG 0.696341 4_2:-64724796 None:intergenic
ATACTGCTATGAAAGAAACG+AGG 0.714480 4_2:-64724473 None:intergenic
AGGGAATACAAGCTTCACTG+AGG 0.754775 4_2:-64725796 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATGTAATCTAAATTA+AGG - chr4_2:64723895-64723914 None:intergenic 10.0%
!! TTTATATAAACTCTTTATTA+TGG + chr4_2:64725129-64725148 Msa0615270:intron 10.0%
!! AAAAAATAGAAGAAAAAACA+GGG + chr4_2:64723741-64723760 Msa0615270:five_prime_UTR 15.0%
!! TATATTTGAATTCTATTATG+AGG + chr4_2:64723831-64723850 Msa0615270:five_prime_UTR 15.0%
!! AGAACAAAAAAAGAAAAATT+AGG - chr4_2:64724103-64724122 None:intergenic 15.0%
!!! ATAATCAATTTTAATGCATA+TGG - chr4_2:64724555-64724574 None:intergenic 15.0%
!!! TGCATTAAAATTGATTATTT+TGG + chr4_2:64724557-64724576 Msa0615270:intron 15.0%
!!! TCAACTATTTTAAAGAAATT+TGG + chr4_2:64726189-64726208 Msa0615270:intron 15.0%
!! ATCAAATTTGATACATATTA+TGG - chr4_2:64726704-64726723 None:intergenic 15.0%
!! AAAAATATGAGAAAGATCAT+TGG - chr4_2:64723608-64723627 None:intergenic 20.0%
!! GAAAAAATAGAAGAAAAAAC+AGG + chr4_2:64723740-64723759 Msa0615270:five_prime_UTR 20.0%
!! AATATACAAATACAGTATAC+AGG - chr4_2:64723817-64723836 None:intergenic 20.0%
!! TGTAATCTAAATTAAGGAAT+GGG - chr4_2:64723889-64723908 None:intergenic 20.0%
!! ATGTAATCTAAATTAAGGAA+TGG - chr4_2:64723890-64723909 None:intergenic 20.0%
!! ATGAAAATTTAATGTAGGTA+GGG - chr4_2:64724523-64724542 None:intergenic 20.0%
!! GAATGATGAAAATTTAATGT+AGG - chr4_2:64724528-64724547 None:intergenic 20.0%
!! ATTATTAAGATTTGTGTTGT+TGG + chr4_2:64724667-64724686 Msa0615270:intron 20.0%
!! ATTTATAACTCTTTCAGATT+GGG + chr4_2:64724758-64724777 Msa0615270:CDS 20.0%
!! ACAAAAGAAAAATGTAGAAA+GGG - chr4_2:64725007-64725026 None:intergenic 20.0%
!! AACAAAAGAAAAATGTAGAA+AGG - chr4_2:64725008-64725027 None:intergenic 20.0%
!! AAATCATGCATATAATGAAA+AGG - chr4_2:64725045-64725064 None:intergenic 20.0%
!!! ATTTCATTGTTTAGTTTTGA+AGG + chr4_2:64725274-64725293 Msa0615270:intron 20.0%
!! ATAATATTTGACATACTTGT+AGG + chr4_2:64725413-64725432 Msa0615270:intron 20.0%
!! TAATATTTGACATACTTGTA+GGG + chr4_2:64725414-64725433 Msa0615270:intron 20.0%
! AGAGACTATACAAAAAAAAG+AGG + chr4_2:64723679-64723698 Msa0615270:five_prime_UTR 25.0%
! CAAAAAAGGAAAGAACATTT+GGG + chr4_2:64723711-64723730 Msa0615270:five_prime_UTR 25.0%
! AAAAAAGGAAAGAACATTTG+GGG + chr4_2:64723712-64723731 Msa0615270:five_prime_UTR 25.0%
! GTAATCTAAATTAAGGAATG+GGG - chr4_2:64723888-64723907 None:intergenic 25.0%
! TAAAATCTCAACAACTGAAT+TGG - chr4_2:64723975-64723994 None:intergenic 25.0%
! CAAAAAAAAGTGAATTGAGT+TGG - chr4_2:64724198-64724217 None:intergenic 25.0%
! GATGAGATAATTAAAATGTC+TGG - chr4_2:64724291-64724310 None:intergenic 25.0%
! AAGTAAATTCTTAAGTACTC+TGG - chr4_2:64724355-64724374 None:intergenic 25.0%
! TTGGTGAAGATTATGATAAA+TGG + chr4_2:64724420-64724439 Msa0615270:CDS 25.0%
! TGGTGAAGATTATGATAAAT+GGG + chr4_2:64724421-64724440 Msa0615270:CDS 25.0%
! GATGAAAATTTAATGTAGGT+AGG - chr4_2:64724524-64724543 None:intergenic 25.0%
! TAAATGAAACAAGATGCAAT+TGG - chr4_2:64724743-64724762 None:intergenic 25.0%
! CATTTATAACTCTTTCAGAT+TGG + chr4_2:64724757-64724776 Msa0615270:CDS 25.0%
! TTTGAACATGGAAAAGAAAA+CGG - chr4_2:64724859-64724878 None:intergenic 25.0%
! CAAAAGAAAAATGTAGAAAG+GGG - chr4_2:64725006-64725025 None:intergenic 25.0%
! TTTCTACTTGAAATGATTAG+AGG - chr4_2:64725158-64725177 None:intergenic 25.0%
!!! TTTAGTTTTGAAGGAATGAA+TGG + chr4_2:64725283-64725302 Msa0615270:intron 25.0%
!!! TTAGTTTTGAAGGAATGAAT+GGG + chr4_2:64725284-64725303 Msa0615270:intron 25.0%
!! GTATCTAAAACAAACTTCTA+AGG - chr4_2:64725323-64725342 None:intergenic 25.0%
! TATACCAATTCAATTGATGT+TGG + chr4_2:64725344-64725363 Msa0615270:intron 25.0%
!!! TTAAGTACTTTGAAATCTTG+AGG + chr4_2:64725383-64725402 Msa0615270:intron 25.0%
! AATATTTGACATACTTGTAG+GGG + chr4_2:64725415-64725434 Msa0615270:intron 25.0%
!! ATTTTGCAAAAGTAACACAT+TGG - chr4_2:64725992-64726011 None:intergenic 25.0%
! TGTTGTTACTGAAGAAAATA+AGG - chr4_2:64726216-64726235 None:intergenic 25.0%
!! AATTGTTACTTATCTACGTT+TGG - chr4_2:64726535-64726554 None:intergenic 25.0%
! TGGTTATTTATAGCAAATTG+AGG + chr4_2:64726574-64726593 Msa0615270:three_prime_UTR 25.0%
!!! AGAGAATTCTTCACTTTTTT+TGG - chr4_2:64726608-64726627 None:intergenic 25.0%
!! TGGATTTTTACACAATGTTA+TGG - chr4_2:64726684-64726703 None:intergenic 25.0%
! TTTTTCCAGATCATCATGTT+CGG + chr4_2:64723627-64723646 Msa0615270:five_prime_UTR 30.0%
GCAAAAAAGGAAAGAACATT+TGG + chr4_2:64723710-64723729 Msa0615270:five_prime_UTR 30.0%
AAAAAGGAAAGAACATTTGG+GGG + chr4_2:64723713-64723732 Msa0615270:five_prime_UTR 30.0%
!! TTGTCTTTTTGTTTAACGTG+TGG + chr4_2:64723854-64723873 Msa0615270:five_prime_UTR 30.0%
!! CTTATTTTTCAGTTGCAAGA+TGG + chr4_2:64724121-64724140 Msa0615270:intron 30.0%
TTTCTTTCATAGCAGTATCT+TGG + chr4_2:64724478-64724497 Msa0615270:CDS 30.0%
AATTTAATGTAGGTAGGGTA+GGG - chr4_2:64724518-64724537 None:intergenic 30.0%
AAATTTAATGTAGGTAGGGT+AGG - chr4_2:64724519-64724538 None:intergenic 30.0%
AAAACGGTGCAATAAGTATT+CGG - chr4_2:64724843-64724862 None:intergenic 30.0%
! AATAGCTCTTAGTTTGAACA+TGG - chr4_2:64724871-64724890 None:intergenic 30.0%
ATGTTCAAACTAAGAGCTAT+TGG + chr4_2:64724870-64724889 Msa0615270:CDS 30.0%
TGTTCAAACTAAGAGCTATT+GGG + chr4_2:64724871-64724890 Msa0615270:CDS 30.0%
TATGTCACAGTTATCTTTAG+AGG - chr4_2:64725073-64725092 None:intergenic 30.0%
CATCATAAGATTCATAACTG+TGG + chr4_2:64725226-64725245 Msa0615270:intron 30.0%
GATACCAACATCAATTGAAT+TGG - chr4_2:64725351-64725370 None:intergenic 30.0%
! TGTGTCTTTCTTGTTTGATA+AGG - chr4_2:64725541-64725560 None:intergenic 30.0%
TGTCAATAGTTCTTCTATAG+AGG - chr4_2:64725699-64725718 None:intergenic 30.0%
ACACATTATTACTTGCATCA+TGG + chr4_2:64725879-64725898 Msa0615270:CDS 30.0%
!!! TTTTTTGGCACATTACTCTT+TGG - chr4_2:64725909-64725928 None:intergenic 30.0%
TAATGTGCCAAAAAATCTCA+AGG + chr4_2:64725914-64725933 Msa0615270:CDS 30.0%
!!! GCTTATACCTTGAGATTTTT+TGG - chr4_2:64725924-64725943 None:intergenic 30.0%
TGGTAAGGTAAACTATACAA+TGG - chr4_2:64726033-64726052 None:intergenic 30.0%
TTTCGATCAACTAATATGTC+TGG - chr4_2:64726059-64726078 None:intergenic 30.0%
CATATTAGTTGATCGAAAAC+TGG + chr4_2:64726061-64726080 Msa0615270:intron 30.0%
TTCCGTTTCATTTATCCATA+TGG + chr4_2:64726124-64726143 Msa0615270:intron 30.0%
TCCGTTTCATTTATCCATAT+GGG + chr4_2:64726125-64726144 Msa0615270:intron 30.0%
ACCCATATGGATAAATGAAA+CGG - chr4_2:64726129-64726148 None:intergenic 30.0%
CTGTTTATCTTTCACCAAAA+AGG - chr4_2:64726243-64726262 None:intergenic 30.0%
ATGTCATGTTCGAAAAAGAA+AGG - chr4_2:64726293-64726312 None:intergenic 30.0%
AATGGTACTTCTGCAAAATA+AGG - chr4_2:64726413-64726432 None:intergenic 30.0%
TGAGATTTGCATTTGTTCAT+TGG + chr4_2:64726554-64726573 Msa0615270:three_prime_UTR 30.0%
! TCTAATTTTCTTTGCTCCTT+TGG + chr4_2:64726637-64726656 Msa0615270:three_prime_UTR 30.0%
TTAAGCAATCTAAAGGAAGA+AGG - chr4_2:64726790-64726809 None:intergenic 30.0%
AGAGGACTAAAGAGCAAAAA+AGG + chr4_2:64723697-64723716 Msa0615270:five_prime_UTR 35.0%
AAAAGGAAAGAACATTTGGG+GGG + chr4_2:64723714-64723733 Msa0615270:five_prime_UTR 35.0%
GTGTCAAACGTGTTTATTAC+AGG - chr4_2:64723772-64723791 None:intergenic 35.0%
ATAAACACGTTTGACACGAA+AGG + chr4_2:64723775-64723794 Msa0615270:five_prime_UTR 35.0%
!! TTCTTAAGTACTCTGGAGTT+TGG - chr4_2:64724348-64724367 None:intergenic 35.0%
AATTGACAACACTATGTGAC+AGG + chr4_2:64724388-64724407 Msa0615270:intron 35.0%
ATGGGTTCATCAACCTATTA+TGG + chr4_2:64724439-64724458 Msa0615270:CDS 35.0%
TGGGTTCATCAACCTATTAT+GGG + chr4_2:64724440-64724459 Msa0615270:CDS 35.0%
TACTGCTATGAAAGAAACGA+GGG - chr4_2:64724475-64724494 None:intergenic 35.0%
ATACTGCTATGAAAGAAACG+AGG - chr4_2:64724476-64724495 None:intergenic 35.0%
!! TCTCTGGAATTGTGTTTAGA+GGG + chr4_2:64724603-64724622 Msa0615270:intron 35.0%
! GAATTGTGTTTAGAGGGTTT+TGG + chr4_2:64724609-64724628 Msa0615270:intron 35.0%
! AAGATTTGTGTTGTTGGAAC+AGG + chr4_2:64724673-64724692 Msa0615270:intron 35.0%
GTGGGTGATTCCAATTATTT+GGG + chr4_2:64724715-64724734 Msa0615270:CDS 35.0%
GCAATTGGAACCCAAATAAT+TGG - chr4_2:64724728-64724747 None:intergenic 35.0%
GTTCAAACTAAGAGCTATTG+GGG + chr4_2:64724872-64724891 Msa0615270:CDS 35.0%
ACATTGCATTATCTTCTCCA+TGG + chr4_2:64724899-64724918 Msa0615270:CDS 35.0%
CAAGTCTTAAGCTATCCATA+GGG - chr4_2:64724940-64724959 None:intergenic 35.0%
ACAAGTCTTAAGCTATCCAT+AGG - chr4_2:64724941-64724960 None:intergenic 35.0%
TACCATGTAGGCTATTAAAG+CGG - chr4_2:64724981-64725000 None:intergenic 35.0%
TAACTGTGACATAGTTTCTC+TGG + chr4_2:64725080-64725099 Msa0615270:intron 35.0%
! TGTGGTGTTAGTGTAAATGA+TGG + chr4_2:64725244-64725263 Msa0615270:intron 35.0%
TTTGAAGGAATGAATGGGAA+TGG + chr4_2:64725289-64725308 Msa0615270:intron 35.0%
TTGAAGGAATGAATGGGAAT+GGG + chr4_2:64725290-64725309 Msa0615270:intron 35.0%
TGATGTTGGTATCAGAAGTA+CGG + chr4_2:64725358-64725377 Msa0615270:intron 35.0%
AAGTCTGTTAGTATTAGACG+AGG + chr4_2:64725444-64725463 Msa0615270:intron 35.0%
ACGAGGAAGAAAGTAATGTT+AGG + chr4_2:64725461-64725480 Msa0615270:intron 35.0%
CGAGGAAGAAAGTAATGTTA+GGG + chr4_2:64725462-64725481 Msa0615270:intron 35.0%
TGTATGGTAGCAATACATGA+AGG - chr4_2:64725509-64725528 None:intergenic 35.0%
GATAAGGCTTATGAGTTGTA+TGG - chr4_2:64725525-64725544 None:intergenic 35.0%
!! GTATTTCTGGCTAGTTGAAA+CGG - chr4_2:64725583-64725602 None:intergenic 35.0%
CGAAACAAGACATGTATTTC+TGG - chr4_2:64725596-64725615 None:intergenic 35.0%
GCAGTAAATGAGTAGTACAT+AGG + chr4_2:64725669-64725688 Msa0615270:intron 35.0%
CACTTTCCCTTCTTAAAATG+TGG + chr4_2:64725943-64725962 Msa0615270:intron 35.0%
!! TGTCGTCCACATTTTAAGAA+GGG - chr4_2:64725952-64725971 None:intergenic 35.0%
!! TTGTCGTCCACATTTTAAGA+AGG - chr4_2:64725953-64725972 None:intergenic 35.0%
AAAAGTAACACATTGGAGCA+AGG - chr4_2:64725985-64726004 None:intergenic 35.0%
!! TAATATGTCTGGCTTTGGTA+AGG - chr4_2:64726048-64726067 None:intergenic 35.0%
! TCAACTAATATGTCTGGCTT+TGG - chr4_2:64726053-64726072 None:intergenic 35.0%
AATGTCACAGTAGTAACTAG+TGG - chr4_2:64726101-64726120 None:intergenic 35.0%
CATATGGGTCTCTTAAACTT+GGG + chr4_2:64726140-64726159 Msa0615270:intron 35.0%
! AGTAACAACAGTGACCTTTT+TGG + chr4_2:64726226-64726245 Msa0615270:intron 35.0%
AGCTCCTACTAAGAATATGA+TGG + chr4_2:64726261-64726280 Msa0615270:intron 35.0%
TGCACCATCATATTCTTAGT+AGG - chr4_2:64726268-64726287 None:intergenic 35.0%
ACTTGTCAGTTGTCTTTCTA+AGG - chr4_2:64726379-64726398 None:intergenic 35.0%
GTACTTCTGCAAAATAAGGA+AGG - chr4_2:64726409-64726428 None:intergenic 35.0%
ACCATTTGAATCATCACTAC+AGG + chr4_2:64726427-64726446 Msa0615270:CDS 35.0%
CCATTTGAATCATCACTACA+GGG + chr4_2:64726428-64726447 Msa0615270:CDS 35.0%
!! ATGGGATAAGGTTTTTGGAA+CGG + chr4_2:64726485-64726504 Msa0615270:CDS 35.0%
TAACCCTCAAAAAACACCAA+AGG - chr4_2:64726656-64726675 None:intergenic 35.0%
CAATCTAAAGGAAGAAGGAA+TGG - chr4_2:64726785-64726804 None:intergenic 35.0%
ATGAGAAAGATCATTGGCAC+AGG - chr4_2:64723602-64723621 None:intergenic 40.0%
! AAGTCTCCCTCTTTGAAAGA+GGG - chr4_2:64724002-64724021 None:intergenic 40.0%
! TAAGTCTCCCTCTTTGAAAG+AGG - chr4_2:64724003-64724022 None:intergenic 40.0%
AAAAGAAGCAGAAGCAGATC+TGG - chr4_2:64724065-64724084 None:intergenic 40.0%
! GAATAAACCCCTTGTTTGTC+AGG + chr4_2:64724169-64724188 Msa0615270:CDS 40.0%
TTGGTTACCTGACAAACAAG+GGG - chr4_2:64724179-64724198 None:intergenic 40.0%
GTTGGTTACCTGACAAACAA+GGG - chr4_2:64724180-64724199 None:intergenic 40.0%
AGTTGGTTACCTGACAAACA+AGG - chr4_2:64724181-64724200 None:intergenic 40.0%
TAATCCTATTCGAGTCGTGT+CGG + chr4_2:64724239-64724258 Msa0615270:intron 40.0%
! TCTGGAGTTTGGTTGAGTAT+CGG - chr4_2:64724337-64724356 None:intergenic 40.0%
TCATCAACCTATTATGGGCA+AGG + chr4_2:64724445-64724464 Msa0615270:CDS 40.0%
CTTTCATAGCAGTATCTTGG+AGG + chr4_2:64724481-64724500 Msa0615270:CDS 40.0%
!! CGACTCAAAAGATTAGTCTC+TGG + chr4_2:64724587-64724606 Msa0615270:intron 40.0%
!! GTCTCTGGAATTGTGTTTAG+AGG + chr4_2:64724602-64724621 Msa0615270:intron 40.0%
GGTGGGTGATTCCAATTATT+TGG + chr4_2:64724714-64724733 Msa0615270:CDS 40.0%
! TGCTACTCGGCATTTTTGTA+TGG + chr4_2:64724810-64724829 Msa0615270:CDS 40.0%
! TAGAAAGGGGTATACCATGT+AGG - chr4_2:64724993-64725012 None:intergenic 40.0%
AATGTTAGGGTCAAATGCAG+AGG + chr4_2:64725475-64725494 Msa0615270:intron 40.0%
!!! TGTGTTTGGAGGCATTTTGT+TGG + chr4_2:64725836-64725855 Msa0615270:CDS 40.0%
!!! GTGTTTGGAGGCATTTTGTT+GGG + chr4_2:64725837-64725856 Msa0615270:CDS 40.0%
CCAAGTTTAAGAGACCCATA+TGG - chr4_2:64726142-64726161 None:intergenic 40.0%
CCATATGGGTCTCTTAAACT+TGG + chr4_2:64726139-64726158 Msa0615270:intron 40.0%
CCCTGTAGTGATGATTCAAA+TGG - chr4_2:64726431-64726450 None:intergenic 40.0%
CCAAAAACCTTATCCCATAG+TGG - chr4_2:64726483-64726502 None:intergenic 40.0%
! CCACTATGGGATAAGGTTTT+TGG + chr4_2:64726480-64726499 Msa0615270:CDS 40.0%
!!! TGCTCCTTTGGTGTTTTTTG+AGG + chr4_2:64726649-64726668 Msa0615270:three_prime_UTR 40.0%
!!! GCTCCTTTGGTGTTTTTTGA+GGG + chr4_2:64726650-64726669 Msa0615270:three_prime_UTR 40.0%
AGGAAGAAGTTACCAGAGAA+AGG - chr4_2:64726758-64726777 None:intergenic 40.0%
TCTAAAGGAAGAAGGAATGG+TGG - chr4_2:64726782-64726801 None:intergenic 40.0%
GGACGACACAGTGGTAAATT+GGG - chr4_2:64723552-64723571 None:intergenic 45.0%
! AGTGAGTGGGGTATGTTTTG+GGG - chr4_2:64723573-64723592 None:intergenic 45.0%
! GAGTGAGTGGGGTATGTTTT+GGG - chr4_2:64723574-64723593 None:intergenic 45.0%
! TGAGTGAGTGGGGTATGTTT+TGG - chr4_2:64723575-64723594 None:intergenic 45.0%
CTGTTCCGAACATGATGATC+TGG - chr4_2:64723635-64723654 None:intergenic 45.0%
TTAGCCTCCCTCTTTCAAAG+AGG + chr4_2:64723992-64724011 Msa0615270:five_prime_UTR 45.0%
TAGCCTCCCTCTTTCAAAGA+GGG + chr4_2:64723993-64724012 Msa0615270:five_prime_UTR 45.0%
CTTCTCTTCTCAGATCTCCT+CGG + chr4_2:64724026-64724045 Msa0615270:five_prime_UTR 45.0%
CTGGAAAATGAAACGAACCG+AGG - chr4_2:64724046-64724065 None:intergenic 45.0%
GACAACACTATGTGACAGGT+TGG + chr4_2:64724392-64724411 Msa0615270:intron 45.0%
!! ATGTGACAGGTTGGTCATCT+TGG + chr4_2:64724401-64724420 Msa0615270:intron 45.0%
CAACCTATTATGGGCAAGGA+AGG + chr4_2:64724449-64724468 Msa0615270:CDS 45.0%
AATGTAGGTAGGGTAGGGTA+GGG - chr4_2:64724513-64724532 None:intergenic 45.0%
TAATGTAGGTAGGGTAGGGT+AGG - chr4_2:64724514-64724533 None:intergenic 45.0%
! TATCCATAGGGTGCTTATGG+TGG - chr4_2:64724928-64724947 None:intergenic 45.0%
! AGCTATCCATAGGGTGCTTA+TGG - chr4_2:64724931-64724950 None:intergenic 45.0%
!! AGACTTGTTTTTCCACCAGC+TGG + chr4_2:64724953-64724972 Msa0615270:CDS 45.0%
! AGCCGCTTTAATAGCCTACA+TGG + chr4_2:64724976-64724995 Msa0615270:CDS 45.0%
TAGGGTCAAATGCAGAGGTT+AGG + chr4_2:64725480-64725499 Msa0615270:intron 45.0%
AGGGAATACAAGCTTCACTG+AGG - chr4_2:64725799-64725818 None:intergenic 45.0%
AACACAGCAGGAGCAATTGA+AGG - chr4_2:64725822-64725841 None:intergenic 45.0%
TCAATTGCTCCTGCTGTGTT+TGG + chr4_2:64725822-64725841 Msa0615270:CDS 45.0%
AAAATGCCTCCAAACACAGC+AGG - chr4_2:64725834-64725853 None:intergenic 45.0%
TTGGCATCACTTCACCACTA+TGG + chr4_2:64726466-64726485 Msa0615270:CDS 45.0%
TGGCATCACTTCACCACTAT+GGG + chr4_2:64726467-64726486 Msa0615270:CDS 45.0%
CACTTCACCACTATGGGATA+AGG + chr4_2:64726473-64726492 Msa0615270:CDS 45.0%
!! TTGGCATCCACTTTTGATGG+AGG - chr4_2:64726516-64726535 None:intergenic 45.0%
!! CGTTTGGCATCCACTTTTGA+TGG - chr4_2:64726519-64726538 None:intergenic 45.0%
AAGGAAGAAGGAATGGTGGT+AGG - chr4_2:64726778-64726797 None:intergenic 45.0%
GGGACGACACAGTGGTAAAT+TGG - chr4_2:64723553-64723572 None:intergenic 50.0%
!!! ATGTTTTGGGGACGACACAG+TGG - chr4_2:64723561-64723580 None:intergenic 50.0%
CACAGGTAAGTGAGTGAGTG+GGG - chr4_2:64723585-64723604 None:intergenic 50.0%
GCACAGGTAAGTGAGTGAGT+GGG - chr4_2:64723586-64723605 None:intergenic 50.0%
TGACACGAAAGGTCGAGAAG+CGG + chr4_2:64723786-64723805 Msa0615270:five_prime_UTR 50.0%
! TCTCCCTCTTTGAAAGAGGG+AGG - chr4_2:64723999-64724018 None:intergenic 50.0%
! AGGTTTTCACGCGAACTGCT+TGG + chr4_2:64724693-64724712 Msa0615270:intron 50.0%
! TTTTCACGCGAACTGCTTGG+TGG + chr4_2:64724696-64724715 Msa0615270:CDS 50.0%
TTTCACGCGAACTGCTTGGT+GGG + chr4_2:64724697-64724716 Msa0615270:CDS 50.0%
AAAGAGGGAGGTGAGGAACA+GGG - chr4_2:64724787-64724806 None:intergenic 50.0%
AAAAGAGGGAGGTGAGGAAC+AGG - chr4_2:64724788-64724807 None:intergenic 50.0%
!! CTCCCTCTTTTCGTGCTACT+CGG + chr4_2:64724797-64724816 Msa0615270:CDS 50.0%
TGCCGAGTAGCACGAAAAGA+GGG - chr4_2:64724802-64724821 None:intergenic 50.0%
ATGCCGAGTAGCACGAAAAG+AGG - chr4_2:64724803-64724822 None:intergenic 50.0%
TTGCCACCATAAGCACCCTA+TGG + chr4_2:64724922-64724941 Msa0615270:CDS 50.0%
TTGTCTGCAACGTCCAGATC+TGG + chr4_2:64725772-64725791 Msa0615270:intron 50.0%
CAGCAGGAGCAATTGAAGGA+GGG - chr4_2:64725818-64725837 None:intergenic 50.0%
ACAGCAGGAGCAATTGAAGG+AGG - chr4_2:64725819-64725838 None:intergenic 50.0%
ATTGCTCCTGCTGTGTTTGG+AGG + chr4_2:64725825-64725844 Msa0615270:CDS 50.0%
GTGAAGTGATGCCAAAGCCA+AGG - chr4_2:64726461-64726480 None:intergenic 50.0%
GGTTCCTCCTCCATCAAAAG+TGG + chr4_2:64726506-64726525 Msa0615270:CDS 50.0%
!! GCATCCACTTTTGATGGAGG+AGG - chr4_2:64726513-64726532 None:intergenic 50.0%
GGCACAGGTAAGTGAGTGAG+TGG - chr4_2:64723587-64723606 None:intergenic 55.0%
GTGTCCGACACGACTCGAAT+AGG - chr4_2:64724246-64724265 None:intergenic 55.0%
GGGCCTTCCTTGCCCATAAT+AGG - chr4_2:64724455-64724474 None:intergenic 55.0%
TAGGTAGGGTAGGGTAGGGT+AGG - chr4_2:64724509-64724528 None:intergenic 55.0%
AGCACGAAAAGAGGGAGGTG+AGG - chr4_2:64724794-64724813 None:intergenic 55.0%
CGAGTAGCACGAAAAGAGGG+AGG - chr4_2:64724799-64724818 None:intergenic 55.0%
GGTGCTTATGGTGGCAACCA+TGG - chr4_2:64724919-64724938 None:intergenic 55.0%
ATTAAAGCGGCTCCAGCTGG+TGG - chr4_2:64724968-64724987 None:intergenic 55.0%
GCTATTAAAGCGGCTCCAGC+TGG - chr4_2:64724971-64724990 None:intergenic 55.0%
GCTTCACTGAGGACCAGATC+TGG - chr4_2:64725788-64725807 None:intergenic 55.0%
TGATGCCAAAGCCAAGGCTC+TGG - chr4_2:64726455-64726474 None:intergenic 55.0%
CCAGAGAAAGGACTGTGCAG+AGG - chr4_2:64726746-64726765 None:intergenic 55.0%
CCTCTGCACAGTCCTTTCTC+TGG + chr4_2:64726743-64726762 Msa0615270:three_prime_UTR 55.0%
CACTACAGGGTCCAGAGCCT+TGG + chr4_2:64726441-64726460 Msa0615270:CDS 60.0%
AGGGTCCAGAGCCTTGGCTT+TGG + chr4_2:64726447-64726466 Msa0615270:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 64723545 64726814 64723545 ID=Msa0615270;Name=Msa0615270
chr4_2 mRNA 64723545 64726814 64723545 ID=Msa0615270-mRNA-1;Parent=Msa0615270;Name=Msa0615270-mRNA-1;_AED=0.03;_eAED=0.03;_QI=510|1|1|1|0.8|0.66|6|269|238
chr4_2 exon 64723545 64724047 64723545 ID=Msa0615270-mRNA-1:exon:18117;Parent=Msa0615270-mRNA-1
chr4_2 exon 64724133 64724190 64724133 ID=Msa0615270-mRNA-1:exon:18118;Parent=Msa0615270-mRNA-1
chr4_2 exon 64724410 64724502 64724410 ID=Msa0615270-mRNA-1:exon:18119;Parent=Msa0615270-mRNA-1
chr4_2 exon 64724695 64724997 64724695 ID=Msa0615270-mRNA-1:exon:18120;Parent=Msa0615270-mRNA-1
chr4_2 exon 64725789 64725935 64725789 ID=Msa0615270-mRNA-1:exon:18121;Parent=Msa0615270-mRNA-1
chr4_2 exon 64726423 64726814 64726423 ID=Msa0615270-mRNA-1:exon:18122;Parent=Msa0615270-mRNA-1
chr4_2 five_prime_UTR 64723545 64724047 64723545 ID=Msa0615270-mRNA-1:five_prime_utr;Parent=Msa0615270-mRNA-1
chr4_2 five_prime_UTR 64724133 64724139 64724133 ID=Msa0615270-mRNA-1:five_prime_utr;Parent=Msa0615270-mRNA-1
chr4_2 CDS 64724140 64724190 64724140 ID=Msa0615270-mRNA-1:cds;Parent=Msa0615270-mRNA-1
chr4_2 CDS 64724410 64724502 64724410 ID=Msa0615270-mRNA-1:cds;Parent=Msa0615270-mRNA-1
chr4_2 CDS 64724695 64724997 64724695 ID=Msa0615270-mRNA-1:cds;Parent=Msa0615270-mRNA-1
chr4_2 CDS 64725789 64725935 64725789 ID=Msa0615270-mRNA-1:cds;Parent=Msa0615270-mRNA-1
chr4_2 CDS 64726423 64726545 64726423 ID=Msa0615270-mRNA-1:cds;Parent=Msa0615270-mRNA-1
chr4_2 three_prime_UTR 64726546 64726814 64726546 ID=Msa0615270-mRNA-1:three_prime_utr;Parent=Msa0615270-mRNA-1
Gene Sequence

>Msa0615270

ATGGTTGCAAACAAATTTGTTGTTGATTTGAATAAACCCCTTGTTTGTCAGGTTGGTCATCTTGGTGAAGATTATGATAAATGGGTTCATCAACCTATTATGGGCAAGGAAGGCCCTCGTTTCTTTCATAGCAGTATCTTGGAGGTTTTCACGCGAACTGCTTGGTGGGTGATTCCAATTATTTGGGTTCCAATTGCATCTTGTTTCATTTATAACTCTTTCAGATTGGGACTCCCTGTTCCTCACCTCCCTCTTTTCGTGCTACTCGGCATTTTTGTATGGACATTGACCGAATACTTATTGCACCGTTTTCTTTTCCATGTTCAAACTAAGAGCTATTGGGGTAATACATTGCATTATCTTCTCCATGGTTGCCACCATAAGCACCCTATGGATAGCTTAAGACTTGTTTTTCCACCAGCTGGAGCCGCTTTAATAGCCTACATGATCTGGTCCTCAGTGAAGCTTGTATTCCCTCCTTCAATTGCTCCTGCTGTGTTTGGAGGCATTTTGTTGGGCTATGTGATGTATGATTGCACACATTATTACTTGCATCATGGTCAACCAAAGAGTAATGTGCCAAAAAATCTCAAGAAGTACCATTTGAATCATCACTACAGGGTCCAGAGCCTTGGCTTTGGCATCACTTCACCACTATGGGATAAGGTTTTTGGAACGGTTCCTCCTCCATCAAAAGTGGATGCCAAACGTAGATAA
Protein sequence

>Msa0615270

MVANKFVVDLNKPLVCQVGHLGEDYDKWVHQPIMGKEGPRFFHSSILEVFTRTAWWVIPIIWVPIASCFIYNSFRLGLPVPHLPLFVLLGIFVWTLTEYLLHRFLFHVQTKSYWGNTLHYLLHGCHHKHPMDSLRLVFPPAGAALIAYMIWSSVKLVFPPSIAPAVFGGILLGYVMYDCTHYYLHHGQPKSNVPKNLKKYHLNHHYRVQSLGFGITSPLWDKVFGTVPPPSKVDAKRR*