AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0701590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0701590 MtrunA17_Chr8g0379441 94.118 170 10 0 1 170 1 170 1.29e-100 286
Msa0701590 MtrunA17_Chr8g0379451 71.951 82 23 0 89 170 1 82 6.70e-36 120
Msa0701590 MtrunA17_Chr5g0437991 35.669 157 86 7 1 154 25 169 3.04e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0701590 AT1G29270.1 38.365 159 88 4 1 156 1 152 5.58e-26 97.4
Msa0701590 AT1G29270.2 41.085 129 67 3 30 156 7 128 7.12e-24 91.3
Msa0701590 AT2G40435.1 35.669 157 86 8 1 152 1 147 1.08e-12 62.8

Find 39 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAAAAGCCTCTAAAAT+TGG 0.235711 4_4:+72999525 None:intergenic
TAGAAAAGTACTCTTGTTAA+TGG 0.243551 4_4:+72999854 None:intergenic
GCAAGAGTTTCATGTGAAAA+TGG 0.245398 4_4:-72999480 Msa0701590:CDS
ATGTTAAGATAGAGAAAATA+AGG 0.252575 4_4:-72999602 Msa0701590:CDS
GACATGTTTGGATGTTTATA+CGG 0.283042 4_4:-72999105 Msa0701590:three_prime_UTR
AGTGAAACCAAACATAAATT+AGG 0.299754 4_4:+72999150 None:intergenic
ACTCTCTTCTTGCGGCTATC+AGG 0.318826 4_4:+72999778 None:intergenic
TTCTCATCCTAATTTATGTT+TGG 0.329997 4_4:-72999157 Msa0701590:three_prime_UTR
GAAGCACTTCTAAAAGCTAT+TGG 0.338458 4_4:-72999396 Msa0701590:CDS
GACATTATTCAATAAGCTAA+TGG 0.375889 4_4:+72999755 None:intergenic
AAACTAAATAAATTTACCTT+TGG 0.379235 4_4:-72999080 Msa0701590:three_prime_UTR
AATATTAGAGATGGCTTGTA+AGG 0.412524 4_4:-73000138 Msa0701590:exon
GGATTAGAAGCTACAAATCA+TGG 0.413552 4_4:+72999057 None:intergenic
TGTTAAGATAGAGAAAATAA+GGG 0.418872 4_4:-72999601 Msa0701590:CDS
CTTGTTAATGGAATTTCTGT+TGG 0.425108 4_4:+72999866 None:intergenic
TGGCTTGTAAGGTGCAGAAA+AGG 0.441174 4_4:-73000127 Msa0701590:CDS
TCACTTAAGATGTAATCAAT+TGG 0.452566 4_4:-72999132 Msa0701590:three_prime_UTR
CATTATTGTTGGAGTTGGTA+AGG 0.458218 4_4:+73000069 None:intergenic
TGGAAAGCAGAGTGGTGAAA+AGG 0.477572 4_4:-72999376 Msa0701590:CDS
GTAAAGGTCACATGTGAGAA+AGG 0.513294 4_4:-72999564 Msa0701590:CDS
GGAAAGCAGAGTGGTGAAAA+GGG 0.519153 4_4:-72999375 Msa0701590:CDS
GAGGATGAAAATCTTGATGT+TGG 0.539252 4_4:-72999429 Msa0701590:CDS
TTCTATGGAAGCTATTATTG+TGG 0.540454 4_4:-72999454 Msa0701590:CDS
GGAAGCTATTATTGTGGCTG+AGG 0.546345 4_4:-72999448 Msa0701590:CDS
GGCTTGTAAGGTGCAGAAAA+GGG 0.547566 4_4:-73000126 Msa0701590:CDS
TATGTAATAGTATTGTTGAA+TGG 0.555192 4_4:-72999231 Msa0701590:three_prime_UTR
AAAGCAGTAAATATTAGAGA+TGG 0.580276 4_4:-73000147 Msa0701590:exon
TGAGAAAGGAGGTGACAAGT+TGG 0.586233 4_4:-72999550 Msa0701590:CDS
GAATAATGTCTATGATAACA+AGG 0.596304 4_4:-72999742 Msa0701590:intron
CAACATTCACAAATCACAAG+AGG 0.597444 4_4:-73000176 None:intergenic
GTTAAGATAGAGAAAATAAG+GGG 0.609952 4_4:-72999600 Msa0701590:CDS
AAGCTACAAATCATGGCCAA+AGG 0.621786 4_4:+72999064 None:intergenic
AGTATTGTTGAATGGTGACA+TGG 0.624222 4_4:-72999223 Msa0701590:three_prime_UTR
GCTAATGGACTCTCTTCTTG+CGG 0.625040 4_4:+72999770 None:intergenic
AAGATAGAGAAAATAAGGGG+AGG 0.627338 4_4:-72999597 Msa0701590:CDS
AGAGATGTGTGTGAATGTTG+AGG 0.643020 4_4:-72999505 Msa0701590:CDS
AAAGCTATTGGAAAGCAGAG+TGG 0.675693 4_4:-72999384 Msa0701590:CDS
AAGGTCACATGTGAGAAAGG+AGG 0.686761 4_4:-72999561 Msa0701590:CDS
GTATTGTTGAATGGTGACAT+GGG 0.693521 4_4:-72999222 Msa0701590:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTTTTTTTTATTATAAC+AGG - chr4_4:72999566-72999585 Msa0701590:CDS 10.0%
!!! ATGTTTTTTTTTATTATAAC+AGG - chr4_4:72999566-72999585 Msa0701590:CDS 10.0%
!!! TGTTAATTTTACATTATTGT+TGG + chr4_4:72999136-72999155 None:intergenic 15.0%
!! GAATGTAAAAAAAGAAAAAA+AGG + chr4_4:72999165-72999184 None:intergenic 15.0%
!! AAACTAAATAAATTTACCTT+TGG - chr4_4:73000111-73000130 Msa0701590:CDS 15.0%
!!! TGTTAATTTTACATTATTGT+TGG + chr4_4:72999136-72999155 None:intergenic 15.0%
!! GAATGTAAAAAAAGAAAAAA+AGG + chr4_4:72999165-72999184 None:intergenic 15.0%
!! AAACTAAATAAATTTACCTT+TGG - chr4_4:73000111-73000130 Msa0701590:CDS 15.0%
!! TCAGTGGAAAAAAATAATAA+TGG + chr4_4:72999196-72999215 None:intergenic 20.0%
!! TAATATTTATATATAGGCCT+TGG + chr4_4:72999524-72999543 None:intergenic 20.0%
!!! AAAAACATGTTATTTTTGCT+TGG + chr4_4:72999554-72999573 None:intergenic 20.0%
!! ATGTTAAGATAGAGAAAATA+AGG - chr4_4:72999589-72999608 Msa0701590:CDS 20.0%
!! TGTTAAGATAGAGAAAATAA+GGG - chr4_4:72999590-72999609 Msa0701590:CDS 20.0%
!!! AAATATGTAAAAACTTGTTG+AGG + chr4_4:72999919-72999938 None:intergenic 20.0%
!! TATGTAATAGTATTGTTGAA+TGG - chr4_4:72999960-72999979 Msa0701590:intron 20.0%
!!! TAAATATTGTTTTGAAAGCT+TGG - chr4_4:72999997-73000016 Msa0701590:intron 20.0%
!! TCAGTGGAAAAAAATAATAA+TGG + chr4_4:72999196-72999215 None:intergenic 20.0%
!! TAATATTTATATATAGGCCT+TGG + chr4_4:72999524-72999543 None:intergenic 20.0%
!!! AAAAACATGTTATTTTTGCT+TGG + chr4_4:72999554-72999573 None:intergenic 20.0%
!! ATGTTAAGATAGAGAAAATA+AGG - chr4_4:72999589-72999608 Msa0701590:CDS 20.0%
!! TGTTAAGATAGAGAAAATAA+GGG - chr4_4:72999590-72999609 Msa0701590:CDS 20.0%
!!! AAATATGTAAAAACTTGTTG+AGG + chr4_4:72999919-72999938 None:intergenic 20.0%
!! TATGTAATAGTATTGTTGAA+TGG - chr4_4:72999960-72999979 Msa0701590:intron 20.0%
!!! TAAATATTGTTTTGAAAGCT+TGG - chr4_4:72999997-73000016 Msa0701590:intron 20.0%
! AAAGCAGTAAATATTAGAGA+TGG - chr4_4:72999044-72999063 Msa0701590:three_prime_UTR 25.0%
!! TTTTACATTATTGTTGGAGT+TGG + chr4_4:72999130-72999149 None:intergenic 25.0%
! GATTAAATTTGTGAAATCAG+TGG + chr4_4:72999212-72999231 None:intergenic 25.0%
!!! TTGTTACTTGTTTGTTTTGA+AGG - chr4_4:72999302-72999321 Msa0701590:three_prime_UTR 25.0%
!!! TAGAAAAGTACTCTTGTTAA+TGG + chr4_4:72999340-72999359 None:intergenic 25.0%
! GACATTATTCAATAAGCTAA+TGG + chr4_4:72999439-72999458 None:intergenic 25.0%
! GAATAATGTCTATGATAACA+AGG - chr4_4:72999449-72999468 Msa0701590:CDS 25.0%
! TATAGAAAAACACAAAACCA+AGG - chr4_4:72999504-72999523 Msa0701590:CDS 25.0%
! GTTAAGATAGAGAAAATAAG+GGG - chr4_4:72999591-72999610 Msa0701590:CDS 25.0%
!!! ATGTGAAAATGGATTTTCTA+TGG - chr4_4:72999722-72999741 Msa0701590:intron 25.0%
! TTCTCATCCTAATTTATGTT+TGG - chr4_4:73000034-73000053 Msa0701590:intron 25.0%
! AGTGAAACCAAACATAAATT+AGG + chr4_4:73000044-73000063 None:intergenic 25.0%
! TCACTTAAGATGTAATCAAT+TGG - chr4_4:73000059-73000078 Msa0701590:intron 25.0%
!! TGAGTGTAAAAATCACATTT+TGG + chr4_4:73000158-73000177 None:intergenic 25.0%
! AAAGCAGTAAATATTAGAGA+TGG - chr4_4:72999044-72999063 Msa0701590:three_prime_UTR 25.0%
!! TTTTACATTATTGTTGGAGT+TGG + chr4_4:72999130-72999149 None:intergenic 25.0%
! GATTAAATTTGTGAAATCAG+TGG + chr4_4:72999212-72999231 None:intergenic 25.0%
!!! TTGTTACTTGTTTGTTTTGA+AGG - chr4_4:72999302-72999321 Msa0701590:three_prime_UTR 25.0%
!!! TAGAAAAGTACTCTTGTTAA+TGG + chr4_4:72999340-72999359 None:intergenic 25.0%
! GACATTATTCAATAAGCTAA+TGG + chr4_4:72999439-72999458 None:intergenic 25.0%
! GAATAATGTCTATGATAACA+AGG - chr4_4:72999449-72999468 Msa0701590:CDS 25.0%
! TATAGAAAAACACAAAACCA+AGG - chr4_4:72999504-72999523 Msa0701590:CDS 25.0%
! GTTAAGATAGAGAAAATAAG+GGG - chr4_4:72999591-72999610 Msa0701590:CDS 25.0%
!!! ATGTGAAAATGGATTTTCTA+TGG - chr4_4:72999722-72999741 Msa0701590:intron 25.0%
! TTCTCATCCTAATTTATGTT+TGG - chr4_4:73000034-73000053 Msa0701590:intron 25.0%
! AGTGAAACCAAACATAAATT+AGG + chr4_4:73000044-73000063 None:intergenic 25.0%
! TCACTTAAGATGTAATCAAT+TGG - chr4_4:73000059-73000078 Msa0701590:intron 25.0%
!! TGAGTGTAAAAATCACATTT+TGG + chr4_4:73000158-73000177 None:intergenic 25.0%
! AATATTAGAGATGGCTTGTA+AGG - chr4_4:72999053-72999072 Msa0701590:three_prime_UTR 30.0%
CTTGTTAATGGAATTTCTGT+TGG + chr4_4:72999328-72999347 None:intergenic 30.0%
!! TCTTCAAAAGCCTCTAAAAT+TGG + chr4_4:72999669-72999688 None:intergenic 30.0%
TTCTATGGAAGCTATTATTG+TGG - chr4_4:72999737-72999756 Msa0701590:intron 30.0%
!!! TCAATTGGTTTTGACATGTT+TGG - chr4_4:73000074-73000093 Msa0701590:CDS 30.0%
GACATGTTTGGATGTTTATA+CGG - chr4_4:73000086-73000105 Msa0701590:CDS 30.0%
TATTATCCTCTTGTGATTTG+TGG + chr4_4:72999024-72999043 None:intergenic 30.0%
! AATATTAGAGATGGCTTGTA+AGG - chr4_4:72999053-72999072 Msa0701590:three_prime_UTR 30.0%
CTTGTTAATGGAATTTCTGT+TGG + chr4_4:72999328-72999347 None:intergenic 30.0%
!! TCTTCAAAAGCCTCTAAAAT+TGG + chr4_4:72999669-72999688 None:intergenic 30.0%
TTCTATGGAAGCTATTATTG+TGG - chr4_4:72999737-72999756 Msa0701590:intron 30.0%
!!! TCAATTGGTTTTGACATGTT+TGG - chr4_4:73000074-73000093 Msa0701590:CDS 30.0%
GACATGTTTGGATGTTTATA+CGG - chr4_4:73000086-73000105 Msa0701590:CDS 30.0%
!! CATTATTGTTGGAGTTGGTA+AGG + chr4_4:72999125-72999144 None:intergenic 35.0%
AAGATAGAGAAAATAAGGGG+AGG - chr4_4:72999594-72999613 Msa0701590:CDS 35.0%
! AATAAGGGGAGGAACTTTTA+TGG - chr4_4:72999605-72999624 Msa0701590:CDS 35.0%
!!! CAAGTTGGTTCCAATTTTAG+AGG - chr4_4:72999656-72999675 Msa0701590:intron 35.0%
GCAAGAGTTTCATGTGAAAA+TGG - chr4_4:72999711-72999730 Msa0701590:intron 35.0%
GAGGATGAAAATCTTGATGT+TGG - chr4_4:72999762-72999781 Msa0701590:CDS 35.0%
! GAAGCACTTCTAAAAGCTAT+TGG - chr4_4:72999795-72999814 Msa0701590:CDS 35.0%
AGTATTGTTGAATGGTGACA+TGG - chr4_4:72999968-72999987 Msa0701590:intron 35.0%
GTATTGTTGAATGGTGACAT+GGG - chr4_4:72999969-72999988 Msa0701590:intron 35.0%
GGATTAGAAGCTACAAATCA+TGG + chr4_4:73000137-73000156 None:intergenic 35.0%
!! CATTATTGTTGGAGTTGGTA+AGG + chr4_4:72999125-72999144 None:intergenic 35.0%
AAGATAGAGAAAATAAGGGG+AGG - chr4_4:72999594-72999613 Msa0701590:CDS 35.0%
! AATAAGGGGAGGAACTTTTA+TGG - chr4_4:72999605-72999624 Msa0701590:CDS 35.0%
!!! CAAGTTGGTTCCAATTTTAG+AGG - chr4_4:72999656-72999675 Msa0701590:intron 35.0%
GCAAGAGTTTCATGTGAAAA+TGG - chr4_4:72999711-72999730 Msa0701590:intron 35.0%
GAGGATGAAAATCTTGATGT+TGG - chr4_4:72999762-72999781 Msa0701590:CDS 35.0%
! GAAGCACTTCTAAAAGCTAT+TGG - chr4_4:72999795-72999814 Msa0701590:CDS 35.0%
AGTATTGTTGAATGGTGACA+TGG - chr4_4:72999968-72999987 Msa0701590:intron 35.0%
GTATTGTTGAATGGTGACAT+GGG - chr4_4:72999969-72999988 Msa0701590:intron 35.0%
GGATTAGAAGCTACAAATCA+TGG + chr4_4:73000137-73000156 None:intergenic 35.0%
! GGGAGGAACTTTTATGGTAA+AGG - chr4_4:72999611-72999630 Msa0701590:intron 40.0%
GTAAAGGTCACATGTGAGAA+AGG - chr4_4:72999627-72999646 Msa0701590:intron 40.0%
AGAGATGTGTGTGAATGTTG+AGG - chr4_4:72999686-72999705 Msa0701590:intron 40.0%
AAAGCTATTGGAAAGCAGAG+TGG - chr4_4:72999807-72999826 Msa0701590:CDS 40.0%
AAGCTACAAATCATGGCCAA+AGG + chr4_4:73000130-73000149 None:intergenic 40.0%
! GGGAGGAACTTTTATGGTAA+AGG - chr4_4:72999611-72999630 Msa0701590:intron 40.0%
GTAAAGGTCACATGTGAGAA+AGG - chr4_4:72999627-72999646 Msa0701590:intron 40.0%
AGAGATGTGTGTGAATGTTG+AGG - chr4_4:72999686-72999705 Msa0701590:intron 40.0%
AAAGCTATTGGAAAGCAGAG+TGG - chr4_4:72999807-72999826 Msa0701590:CDS 40.0%
AAGCTACAAATCATGGCCAA+AGG + chr4_4:73000130-73000149 None:intergenic 40.0%
! TGGCTTGTAAGGTGCAGAAA+AGG - chr4_4:72999064-72999083 Msa0701590:three_prime_UTR 45.0%
GGCTTGTAAGGTGCAGAAAA+GGG - chr4_4:72999065-72999084 Msa0701590:three_prime_UTR 45.0%
!! GCTAATGGACTCTCTTCTTG+CGG + chr4_4:72999424-72999443 None:intergenic 45.0%
AAGGTCACATGTGAGAAAGG+AGG - chr4_4:72999630-72999649 Msa0701590:intron 45.0%
TGAGAAAGGAGGTGACAAGT+TGG - chr4_4:72999641-72999660 Msa0701590:intron 45.0%
GGAAGCTATTATTGTGGCTG+AGG - chr4_4:72999743-72999762 Msa0701590:CDS 45.0%
TGGAAAGCAGAGTGGTGAAA+AGG - chr4_4:72999815-72999834 Msa0701590:CDS 45.0%
GGAAAGCAGAGTGGTGAAAA+GGG - chr4_4:72999816-72999835 Msa0701590:CDS 45.0%
! TGGCTTGTAAGGTGCAGAAA+AGG - chr4_4:72999064-72999083 Msa0701590:three_prime_UTR 45.0%
GGCTTGTAAGGTGCAGAAAA+GGG - chr4_4:72999065-72999084 Msa0701590:three_prime_UTR 45.0%
!! GCTAATGGACTCTCTTCTTG+CGG + chr4_4:72999424-72999443 None:intergenic 45.0%
AAGGTCACATGTGAGAAAGG+AGG - chr4_4:72999630-72999649 Msa0701590:intron 45.0%
TGAGAAAGGAGGTGACAAGT+TGG - chr4_4:72999641-72999660 Msa0701590:intron 45.0%
GGAAGCTATTATTGTGGCTG+AGG - chr4_4:72999743-72999762 Msa0701590:CDS 45.0%
TGGAAAGCAGAGTGGTGAAA+AGG - chr4_4:72999815-72999834 Msa0701590:CDS 45.0%
GGAAAGCAGAGTGGTGAAAA+GGG - chr4_4:72999816-72999835 Msa0701590:CDS 45.0%
!!! TTTTAGTAATATTTATATAT+AGG + chr4_4:72999530-72999549 None:intergenic 5.0%
!!! TTTTAGTAATATTTATATAT+AGG + chr4_4:72999530-72999549 None:intergenic 5.0%
ACTCTCTTCTTGCGGCTATC+AGG + chr4_4:72999416-72999435 None:intergenic 50.0%
ACTCTCTTCTTGCGGCTATC+AGG + chr4_4:72999416-72999435 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 72999022 73000191 72999022 ID=Msa0701590;Name=Msa0701590
chr4_4 mRNA 72999022 73000191 72999022 ID=Msa0701590-mRNA-1;Parent=Msa0701590;Name=Msa0701590-mRNA-1;_AED=0.07;_eAED=0.07;_QI=41|1|1|1|1|1|3|319|170
chr4_4 exon 72999022 72999625 72999022 ID=Msa0701590-mRNA-1:exon:22369;Parent=Msa0701590-mRNA-1
chr4_4 exon 72999743 72999889 72999743 ID=Msa0701590-mRNA-1:exon:22368;Parent=Msa0701590-mRNA-1
chr4_4 exon 73000070 73000191 73000070 ID=Msa0701590-mRNA-1:exon:22367;Parent=Msa0701590-mRNA-1
chr4_4 five_prime_UTR 73000151 73000191 73000151 ID=Msa0701590-mRNA-1:five_prime_utr;Parent=Msa0701590-mRNA-1
chr4_4 CDS 73000070 73000150 73000070 ID=Msa0701590-mRNA-1:cds;Parent=Msa0701590-mRNA-1
chr4_4 CDS 72999743 72999889 72999743 ID=Msa0701590-mRNA-1:cds;Parent=Msa0701590-mRNA-1
chr4_4 CDS 72999341 72999625 72999341 ID=Msa0701590-mRNA-1:cds;Parent=Msa0701590-mRNA-1
chr4_4 three_prime_UTR 72999022 72999340 72999022 ID=Msa0701590-mRNA-1:three_prime_utr;Parent=Msa0701590-mRNA-1
Gene Sequence

>Msa0701590

ATGGCTTGTAAGGTGCAGAAAAGGGTATCACTTCGCAGAAGACTTCACATTCTGCGAGTCCTTACCAACTCCAACAATAATGCCAACAGAAATTCCATTAACAAGAGTACTTTTCTACACATTCATAAGCTAAAGCTTGCACTAGAAACTCTCAAAAGAGAGTATGAAAACCTGATAGCCGCAAGAAGAGAGTCCATTAGCTTATTGAATAATGTCTATGATAACAAGGATGTTAAGATAGAGAAAATAAGGGGAGGAACTTTTATGGTAAAGGTCACATGTGAGAAAGGAGGTGACAAGTTGGTTCCAATTTTAGAGGCTTTTGAAGAGATGTGTGTGAATGTTGAGGAAGCAAGAGTTTCATGTGAAAATGGATTTTCTATGGAAGCTATTATTGTGGCTGAGGATGAAAATCTTGATGTTGGAGATGTTAATGAAGCACTTCTAAAAGCTATTGGAAAGCAGAGTGGTGAAAAGGGTTCAAAAGAGCTTGACAACTGTAGTAATTTGTGA

Protein sequence

>Msa0701590

MACKVQKRVSLRRRLHILRVLTNSNNNANRNSINKSTFLHIHKLKLALETLKREYENLIAARRESISLLNNVYDNKDVKIEKIRGGTFMVKVTCEKGGDKLVPILEAFEEMCVNVEEARVSCENGFSMEAIIVAEDENLDVGDVNEALLKAIGKQSGEKGSKELDNCSNL*