AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0565090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0565090 MtrunA17_Chr8g0379441 94.118 170 10 0 1 170 1 170 1.40e-100 286
Msa0565090 MtrunA17_Chr8g0379451 71.951 82 23 0 89 170 1 82 4.38e-36 120
Msa0565090 MtrunA17_Chr5g0437991 35.484 155 89 6 1 154 25 169 2.82e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0565090 AT1G29270.1 38.365 159 88 4 1 156 1 152 7.38e-26 97.1
Msa0565090 AT1G29270.2 41.085 129 67 3 30 156 7 128 1.21e-23 90.5
Msa0565090 AT2G40435.1 35.669 157 86 8 1 152 1 147 5.85e-13 63.5
Msa0565090 AT3G56220.1 32.298 161 95 6 1 156 1 152 7.85e-11 57.8

Find 38 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAAAAGCCTCTAAAAT+TGG 0.235711 4_1:-66007428 None:intergenic
TAGAAAAGTACTCTTGTTAA+TGG 0.243551 4_1:-66007098 None:intergenic
GCAAGAGTTTCATGTGAAAA+TGG 0.245398 4_1:+66007473 Msa0565090:CDS
ATGTTAAGATAGAGAAAATA+AGG 0.252575 4_1:+66007351 Msa0565090:CDS
GACATGTTTGGATGTTTATA+CGG 0.283042 4_1:+66007848 Msa0565090:three_prime_UTR
AGTGAAACCAAACATAAATT+AGG 0.299754 4_1:-66007803 None:intergenic
ACTCTCTTCTTGCGGCTATC+AGG 0.318826 4_1:-66007174 None:intergenic
TTCTCATCCTAATTTATGTT+TGG 0.329997 4_1:+66007796 Msa0565090:three_prime_UTR
GAAGCACTTCTAAAAGCTAT+TGG 0.338458 4_1:+66007557 Msa0565090:CDS
GACATTATTCAATAAGCTAA+TGG 0.375889 4_1:-66007197 None:intergenic
GGTAAAACTAAATTTACCTT+TGG 0.385623 4_1:+66007869 Msa0565090:three_prime_UTR
AATATTAGAGATGGCTTGTA+AGG 0.412524 4_1:+66006810 Msa0565090:exon
TGTTAAGATAGAGAAAATAA+GGG 0.418872 4_1:+66007352 Msa0565090:CDS
CTTGTTAATGGAATTTCTGT+TGG 0.425108 4_1:-66007086 None:intergenic
TGGCTTGTAAGGTGCAGAAA+AGG 0.441174 4_1:+66006821 Msa0565090:CDS
GGATCAGAAGCTACAAATCA+TGG 0.448763 4_1:-66007892 None:intergenic
TCACTTAAGATGTAATCAAT+TGG 0.452566 4_1:+66007821 Msa0565090:three_prime_UTR
CATTATTGTTGGAGTTGGTA+AGG 0.458218 4_1:-66006879 None:intergenic
TGGAAAGCAGAGTGGTGAAA+AGG 0.477572 4_1:+66007577 Msa0565090:CDS
GTAAAGGTCACATGTGAGAA+AGG 0.513294 4_1:+66007389 Msa0565090:CDS
GGAAAGCAGAGTGGTGAAAA+GGG 0.519153 4_1:+66007578 Msa0565090:CDS
TTCTATGGAAGCTATTATTG+TGG 0.540454 4_1:+66007499 Msa0565090:CDS
GGAAGCTATTATTGTGGCTG+AGG 0.546345 4_1:+66007505 Msa0565090:CDS
GGCTTGTAAGGTGCAGAAAA+GGG 0.547566 4_1:+66006822 Msa0565090:CDS
TATGTAATAGTATTGTTGAA+TGG 0.555192 4_1:+66007722 Msa0565090:three_prime_UTR
AAAGCAGTAAATATTAGAGA+TGG 0.580276 4_1:+66006801 Msa0565090:exon
TGAGAAAGGAGGTGACAAGT+TGG 0.586233 4_1:+66007403 Msa0565090:CDS
GAATAATGTCTATGATAACA+AGG 0.596304 4_1:+66007210 Msa0565090:CDS
CAACATTCACAAATCACAAG+AGG 0.597444 4_1:+66006772 None:intergenic
GTTAAGATAGAGAAAATAAG+GGG 0.609952 4_1:+66007353 Msa0565090:CDS
AGTATTGTTGAATGGTGACA+TGG 0.624222 4_1:+66007730 Msa0565090:three_prime_UTR
GCTAATGGACTCTCTTCTTG+CGG 0.625040 4_1:-66007182 None:intergenic
AAGATAGAGAAAATAAGGGG+AGG 0.627338 4_1:+66007356 Msa0565090:CDS
AAGCTACAAATCATGGCCAA+AGG 0.639180 4_1:-66007885 None:intergenic
AGAGATGTGTGTGAATGTTG+AGG 0.643020 4_1:+66007448 Msa0565090:CDS
AAAGCTATTGGAAAGCAGAG+TGG 0.675693 4_1:+66007569 Msa0565090:CDS
AAGGTCACATGTGAGAAAGG+AGG 0.686761 4_1:+66007392 Msa0565090:CDS
GTATTGTTGAATGGTGACAT+GGG 0.693521 4_1:+66007731 Msa0565090:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTTTTTTTTATTATAAC+AGG + chr4_1:66007328-66007347 Msa0565090:intron 10.0%
!!! ATGTTTTTTTTTATTATAAC+AGG + chr4_1:66007328-66007347 Msa0565090:intron 10.0%
!!! TGTTAATTTTACATTATTGT+TGG - chr4_1:66006893-66006912 None:intergenic 15.0%
!! GAATGTAAAAAAAGAAAAAA+AGG - chr4_1:66006922-66006941 None:intergenic 15.0%
!!! TGTTAATTTTACATTATTGT+TGG - chr4_1:66006893-66006912 None:intergenic 15.0%
!! GAATGTAAAAAAAGAAAAAA+AGG - chr4_1:66006922-66006941 None:intergenic 15.0%
!! TCAGTGGAAAAAAATAATAA+TGG - chr4_1:66006953-66006972 None:intergenic 20.0%
!! TAATATTTATATATAGGCCT+TGG - chr4_1:66007286-66007305 None:intergenic 20.0%
!!! AAAAACATGTTATTTTTGCT+TGG - chr4_1:66007316-66007335 None:intergenic 20.0%
!! ATGTTAAGATAGAGAAAATA+AGG + chr4_1:66007351-66007370 Msa0565090:CDS 20.0%
!! TGTTAAGATAGAGAAAATAA+GGG + chr4_1:66007352-66007371 Msa0565090:CDS 20.0%
!!! AAATATGTAAAAACTTGTTG+AGG - chr4_1:66007681-66007700 None:intergenic 20.0%
!! TATGTAATAGTATTGTTGAA+TGG + chr4_1:66007722-66007741 Msa0565090:three_prime_UTR 20.0%
!!! TAAATATTGTTTTGAAAGCT+TGG + chr4_1:66007759-66007778 Msa0565090:three_prime_UTR 20.0%
!! TCAGTGGAAAAAAATAATAA+TGG - chr4_1:66006953-66006972 None:intergenic 20.0%
!! TAATATTTATATATAGGCCT+TGG - chr4_1:66007286-66007305 None:intergenic 20.0%
!!! AAAAACATGTTATTTTTGCT+TGG - chr4_1:66007316-66007335 None:intergenic 20.0%
!! ATGTTAAGATAGAGAAAATA+AGG + chr4_1:66007351-66007370 Msa0565090:CDS 20.0%
!! TGTTAAGATAGAGAAAATAA+GGG + chr4_1:66007352-66007371 Msa0565090:CDS 20.0%
!!! AAATATGTAAAAACTTGTTG+AGG - chr4_1:66007681-66007700 None:intergenic 20.0%
!! TATGTAATAGTATTGTTGAA+TGG + chr4_1:66007722-66007741 Msa0565090:three_prime_UTR 20.0%
!!! TAAATATTGTTTTGAAAGCT+TGG + chr4_1:66007759-66007778 Msa0565090:three_prime_UTR 20.0%
! AAAGCAGTAAATATTAGAGA+TGG + chr4_1:66006801-66006820 Msa0565090:exon 25.0%
!! TTTTACATTATTGTTGGAGT+TGG - chr4_1:66006887-66006906 None:intergenic 25.0%
! GATTAAATTTGTGAAATCAG+TGG - chr4_1:66006969-66006988 None:intergenic 25.0%
!!! TTGTTACTTGTTTGTTTTGA+AGG + chr4_1:66007063-66007082 Msa0565090:intron 25.0%
!!! TAGAAAAGTACTCTTGTTAA+TGG - chr4_1:66007101-66007120 None:intergenic 25.0%
! GACATTATTCAATAAGCTAA+TGG - chr4_1:66007200-66007219 None:intergenic 25.0%
! GAATAATGTCTATGATAACA+AGG + chr4_1:66007210-66007229 Msa0565090:CDS 25.0%
! TATAGAAAAACACAAAACCA+AGG + chr4_1:66007266-66007285 Msa0565090:intron 25.0%
! GTTAAGATAGAGAAAATAAG+GGG + chr4_1:66007353-66007372 Msa0565090:CDS 25.0%
!!! ATGTGAAAATGGATTTTCTA+TGG + chr4_1:66007484-66007503 Msa0565090:CDS 25.0%
! TTCTCATCCTAATTTATGTT+TGG + chr4_1:66007796-66007815 Msa0565090:three_prime_UTR 25.0%
! AGTGAAACCAAACATAAATT+AGG - chr4_1:66007806-66007825 None:intergenic 25.0%
! TCACTTAAGATGTAATCAAT+TGG + chr4_1:66007821-66007840 Msa0565090:three_prime_UTR 25.0%
! GGTAAAACTAAATTTACCTT+TGG + chr4_1:66007869-66007888 Msa0565090:three_prime_UTR 25.0%
!! TGAGTGTAAAAATCACATTT+TGG - chr4_1:66007916-66007935 None:intergenic 25.0%
! AAAGCAGTAAATATTAGAGA+TGG + chr4_1:66006801-66006820 Msa0565090:exon 25.0%
!! TTTTACATTATTGTTGGAGT+TGG - chr4_1:66006887-66006906 None:intergenic 25.0%
! GATTAAATTTGTGAAATCAG+TGG - chr4_1:66006969-66006988 None:intergenic 25.0%
!!! TTGTTACTTGTTTGTTTTGA+AGG + chr4_1:66007063-66007082 Msa0565090:intron 25.0%
!!! TAGAAAAGTACTCTTGTTAA+TGG - chr4_1:66007101-66007120 None:intergenic 25.0%
! GACATTATTCAATAAGCTAA+TGG - chr4_1:66007200-66007219 None:intergenic 25.0%
! GAATAATGTCTATGATAACA+AGG + chr4_1:66007210-66007229 Msa0565090:CDS 25.0%
! TATAGAAAAACACAAAACCA+AGG + chr4_1:66007266-66007285 Msa0565090:intron 25.0%
! GTTAAGATAGAGAAAATAAG+GGG + chr4_1:66007353-66007372 Msa0565090:CDS 25.0%
!!! ATGTGAAAATGGATTTTCTA+TGG + chr4_1:66007484-66007503 Msa0565090:CDS 25.0%
! TTCTCATCCTAATTTATGTT+TGG + chr4_1:66007796-66007815 Msa0565090:three_prime_UTR 25.0%
! AGTGAAACCAAACATAAATT+AGG - chr4_1:66007806-66007825 None:intergenic 25.0%
! TCACTTAAGATGTAATCAAT+TGG + chr4_1:66007821-66007840 Msa0565090:three_prime_UTR 25.0%
! GGTAAAACTAAATTTACCTT+TGG + chr4_1:66007869-66007888 Msa0565090:three_prime_UTR 25.0%
!! TGAGTGTAAAAATCACATTT+TGG - chr4_1:66007916-66007935 None:intergenic 25.0%
! AATATTAGAGATGGCTTGTA+AGG + chr4_1:66006810-66006829 Msa0565090:exon 30.0%
CTTGTTAATGGAATTTCTGT+TGG - chr4_1:66007089-66007108 None:intergenic 30.0%
!! TCTTCAAAAGCCTCTAAAAT+TGG - chr4_1:66007431-66007450 None:intergenic 30.0%
TTCTATGGAAGCTATTATTG+TGG + chr4_1:66007499-66007518 Msa0565090:CDS 30.0%
!!! TCAATTGGTTTTGACATGTT+TGG + chr4_1:66007836-66007855 Msa0565090:three_prime_UTR 30.0%
GACATGTTTGGATGTTTATA+CGG + chr4_1:66007848-66007867 Msa0565090:three_prime_UTR 30.0%
TATTATCCTCTTGTGATTTG+TGG - chr4_1:66006781-66006800 None:intergenic 30.0%
! AATATTAGAGATGGCTTGTA+AGG + chr4_1:66006810-66006829 Msa0565090:exon 30.0%
CTTGTTAATGGAATTTCTGT+TGG - chr4_1:66007089-66007108 None:intergenic 30.0%
!! TCTTCAAAAGCCTCTAAAAT+TGG - chr4_1:66007431-66007450 None:intergenic 30.0%
TTCTATGGAAGCTATTATTG+TGG + chr4_1:66007499-66007518 Msa0565090:CDS 30.0%
!!! TCAATTGGTTTTGACATGTT+TGG + chr4_1:66007836-66007855 Msa0565090:three_prime_UTR 30.0%
GACATGTTTGGATGTTTATA+CGG + chr4_1:66007848-66007867 Msa0565090:three_prime_UTR 30.0%
!! CATTATTGTTGGAGTTGGTA+AGG - chr4_1:66006882-66006901 None:intergenic 35.0%
AAGATAGAGAAAATAAGGGG+AGG + chr4_1:66007356-66007375 Msa0565090:CDS 35.0%
! AATAAGGGGAGGAACTTTTA+TGG + chr4_1:66007367-66007386 Msa0565090:CDS 35.0%
!!! CAAGTTGGTTCCAATTTTAG+AGG + chr4_1:66007418-66007437 Msa0565090:CDS 35.0%
GCAAGAGTTTCATGTGAAAA+TGG + chr4_1:66007473-66007492 Msa0565090:CDS 35.0%
! GAAGCACTTCTAAAAGCTAT+TGG + chr4_1:66007557-66007576 Msa0565090:CDS 35.0%
AGTATTGTTGAATGGTGACA+TGG + chr4_1:66007730-66007749 Msa0565090:three_prime_UTR 35.0%
GTATTGTTGAATGGTGACAT+GGG + chr4_1:66007731-66007750 Msa0565090:three_prime_UTR 35.0%
!! CATTATTGTTGGAGTTGGTA+AGG - chr4_1:66006882-66006901 None:intergenic 35.0%
AAGATAGAGAAAATAAGGGG+AGG + chr4_1:66007356-66007375 Msa0565090:CDS 35.0%
! AATAAGGGGAGGAACTTTTA+TGG + chr4_1:66007367-66007386 Msa0565090:CDS 35.0%
!!! CAAGTTGGTTCCAATTTTAG+AGG + chr4_1:66007418-66007437 Msa0565090:CDS 35.0%
GCAAGAGTTTCATGTGAAAA+TGG + chr4_1:66007473-66007492 Msa0565090:CDS 35.0%
! GAAGCACTTCTAAAAGCTAT+TGG + chr4_1:66007557-66007576 Msa0565090:CDS 35.0%
AGTATTGTTGAATGGTGACA+TGG + chr4_1:66007730-66007749 Msa0565090:three_prime_UTR 35.0%
GTATTGTTGAATGGTGACAT+GGG + chr4_1:66007731-66007750 Msa0565090:three_prime_UTR 35.0%
! GGGAGGAACTTTTATGGTAA+AGG + chr4_1:66007373-66007392 Msa0565090:CDS 40.0%
GTAAAGGTCACATGTGAGAA+AGG + chr4_1:66007389-66007408 Msa0565090:CDS 40.0%
AGAGATGTGTGTGAATGTTG+AGG + chr4_1:66007448-66007467 Msa0565090:CDS 40.0%
AAAGCTATTGGAAAGCAGAG+TGG + chr4_1:66007569-66007588 Msa0565090:CDS 40.0%
AAGCTACAAATCATGGCCAA+AGG - chr4_1:66007888-66007907 None:intergenic 40.0%
GGATCAGAAGCTACAAATCA+TGG - chr4_1:66007895-66007914 None:intergenic 40.0%
! GGGAGGAACTTTTATGGTAA+AGG + chr4_1:66007373-66007392 Msa0565090:CDS 40.0%
GTAAAGGTCACATGTGAGAA+AGG + chr4_1:66007389-66007408 Msa0565090:CDS 40.0%
AGAGATGTGTGTGAATGTTG+AGG + chr4_1:66007448-66007467 Msa0565090:CDS 40.0%
AAAGCTATTGGAAAGCAGAG+TGG + chr4_1:66007569-66007588 Msa0565090:CDS 40.0%
AAGCTACAAATCATGGCCAA+AGG - chr4_1:66007888-66007907 None:intergenic 40.0%
GGATCAGAAGCTACAAATCA+TGG - chr4_1:66007895-66007914 None:intergenic 40.0%
! TGGCTTGTAAGGTGCAGAAA+AGG + chr4_1:66006821-66006840 Msa0565090:CDS 45.0%
GGCTTGTAAGGTGCAGAAAA+GGG + chr4_1:66006822-66006841 Msa0565090:CDS 45.0%
!! GCTAATGGACTCTCTTCTTG+CGG - chr4_1:66007185-66007204 None:intergenic 45.0%
AAGGTCACATGTGAGAAAGG+AGG + chr4_1:66007392-66007411 Msa0565090:CDS 45.0%
TGAGAAAGGAGGTGACAAGT+TGG + chr4_1:66007403-66007422 Msa0565090:CDS 45.0%
GGAAGCTATTATTGTGGCTG+AGG + chr4_1:66007505-66007524 Msa0565090:CDS 45.0%
TGGAAAGCAGAGTGGTGAAA+AGG + chr4_1:66007577-66007596 Msa0565090:CDS 45.0%
GGAAAGCAGAGTGGTGAAAA+GGG + chr4_1:66007578-66007597 Msa0565090:CDS 45.0%
! TGGCTTGTAAGGTGCAGAAA+AGG + chr4_1:66006821-66006840 Msa0565090:CDS 45.0%
GGCTTGTAAGGTGCAGAAAA+GGG + chr4_1:66006822-66006841 Msa0565090:CDS 45.0%
!! GCTAATGGACTCTCTTCTTG+CGG - chr4_1:66007185-66007204 None:intergenic 45.0%
AAGGTCACATGTGAGAAAGG+AGG + chr4_1:66007392-66007411 Msa0565090:CDS 45.0%
TGAGAAAGGAGGTGACAAGT+TGG + chr4_1:66007403-66007422 Msa0565090:CDS 45.0%
GGAAGCTATTATTGTGGCTG+AGG + chr4_1:66007505-66007524 Msa0565090:CDS 45.0%
TGGAAAGCAGAGTGGTGAAA+AGG + chr4_1:66007577-66007596 Msa0565090:CDS 45.0%
GGAAAGCAGAGTGGTGAAAA+GGG + chr4_1:66007578-66007597 Msa0565090:CDS 45.0%
!!! TTTTAGTAATATTTATATAT+AGG - chr4_1:66007292-66007311 None:intergenic 5.0%
!!! TTTTAGTAATATTTATATAT+AGG - chr4_1:66007292-66007311 None:intergenic 5.0%
ACTCTCTTCTTGCGGCTATC+AGG - chr4_1:66007177-66007196 None:intergenic 50.0%
ACTCTCTTCTTGCGGCTATC+AGG - chr4_1:66007177-66007196 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 66006779 66007949 66006779 ID=Msa0565090;Name=Msa0565090
chr4_1 mRNA 66006779 66007949 66006779 ID=Msa0565090-mRNA-1;Parent=Msa0565090;Name=Msa0565090-mRNA-1;_AED=0.02;_eAED=0.02;_QI=41|1|1|1|1|1|3|315|170
chr4_1 exon 66006779 66006900 66006779 ID=Msa0565090-mRNA-1:exon:21315;Parent=Msa0565090-mRNA-1
chr4_1 exon 66007085 66007231 66007085 ID=Msa0565090-mRNA-1:exon:21316;Parent=Msa0565090-mRNA-1
chr4_1 exon 66007350 66007949 66007350 ID=Msa0565090-mRNA-1:exon:21317;Parent=Msa0565090-mRNA-1
chr4_1 five_prime_UTR 66006779 66006819 66006779 ID=Msa0565090-mRNA-1:five_prime_utr;Parent=Msa0565090-mRNA-1
chr4_1 CDS 66006820 66006900 66006820 ID=Msa0565090-mRNA-1:cds;Parent=Msa0565090-mRNA-1
chr4_1 CDS 66007085 66007231 66007085 ID=Msa0565090-mRNA-1:cds;Parent=Msa0565090-mRNA-1
chr4_1 CDS 66007350 66007634 66007350 ID=Msa0565090-mRNA-1:cds;Parent=Msa0565090-mRNA-1
chr4_1 three_prime_UTR 66007635 66007949 66007635 ID=Msa0565090-mRNA-1:three_prime_utr;Parent=Msa0565090-mRNA-1
Gene Sequence

>Msa0565090

ATGGCTTGTAAGGTGCAGAAAAGGGTATCACTTCGCAGAAGACTTCACATTCTGCGAGTCCTTACCAACTCCAACAATAATGCCAACAGAAATTCCATTAACAAGAGTACTTTTCTACACATTCATAAGCTAAAGCTTGCACTAGAAACTCTCAAAAGAGAGTATGAAAACCTGATAGCCGCAAGAAGAGAGTCCATTAGCTTATTGAATAATGTCTATGATAACAAGGATGTTAAGATAGAGAAAATAAGGGGAGGAACTTTTATGGTAAAGGTCACATGTGAGAAAGGAGGTGACAAGTTGGTTCCAATTTTAGAGGCTTTTGAAGAGATGTGTGTGAATGTTGAGGAAGCAAGAGTTTCATGTGAAAATGGATTTTCTATGGAAGCTATTATTGTGGCTGAGGATGAAAATCTTGATGTTAGAGATGTTAATGAAGCACTTCTAAAAGCTATTGGAAAGCAGAGTGGTGAAAAGGGTTCAAAAGAGCTTGACAACTGTAGTAATTTGTGA

Protein sequence

>Msa0565090

MACKVQKRVSLRRRLHILRVLTNSNNNANRNSINKSTFLHIHKLKLALETLKREYENLIAARRESISLLNNVYDNKDVKIEKIRGGTFMVKVTCEKGGDKLVPILEAFEEMCVNVEEARVSCENGFSMEAIIVAEDENLDVRDVNEALLKAIGKQSGEKGSKELDNCSNL*