AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0837500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0837500 MtrunA17_Chr5g0404311 95.437 767 35 0 1 767 1 767 0.0 1477
Msa0837500 MtrunA17_Chr8g0373031 56.596 758 316 6 1 755 1 748 0.0 844
Msa0837500 MtrunA17_Chr8g0377151 31.198 718 447 13 19 713 19 712 1.04e-101 330
Msa0837500 MtrunA17_Chr3g0122721 30.406 763 490 15 10 753 9 749 2.53e-99 324
Msa0837500 MtrunA17_Chr1g0155261 41.627 209 121 1 19 226 16 224 1.60e-38 143
Msa0837500 MtrunA17_Chr1g0183781 28.257 499 281 17 271 713 239 716 3.00e-30 127
Msa0837500 MtrunA17_Chr7g0237931 26.196 439 250 14 274 709 238 605 1.22e-27 119
Msa0837500 MtrunA17_Chr7g0275081 37.857 140 85 1 225 362 28 167 4.77e-27 115
Msa0837500 MtrunA17_Chr4g0044411 49.412 85 43 0 264 348 264 348 8.71e-24 107
Msa0837500 MtrunA17_Chr7g0234671 24.176 455 249 14 242 662 54 446 8.82e-23 102
Msa0837500 MtrunA17_Chr1g0199321 53.333 75 35 0 274 348 248 322 3.31e-22 102
Msa0837500 MtrunA17_Chr2g0284461 21.510 437 288 10 274 710 293 674 3.97e-22 102
Msa0837500 MtrunA17_Chr4g0031431 24.847 326 207 9 29 348 37 330 1.14e-21 100
Msa0837500 MtrunA17_Chr3g0106931 48.889 90 45 1 266 354 225 314 2.27e-21 99.8
Msa0837500 MtrunA17_Chr1g0189811 45.455 77 42 0 274 350 64 140 1.41e-20 95.5
Msa0837500 MtrunA17_Chr7g0248251 37.903 124 64 2 253 367 258 377 4.20e-20 95.1
Msa0837500 MtrunA17_Chr4g0013091 24.070 457 270 15 266 710 306 697 1.61e-19 93.6
Msa0837500 MtrunA17_Chr7g0261371 47.436 78 41 0 271 348 239 316 2.01e-19 93.6
Msa0837500 MtrunA17_Chr7g0261371 29.655 290 162 12 451 713 516 790 5.56e-12 69.3
Msa0837500 MtrunA17_Chr5g0437411 48.649 74 38 0 274 347 294 367 1.89e-18 90.1
Msa0837500 MtrunA17_Chr5g0413681 43.902 82 46 0 275 356 282 363 2.27e-17 86.7
Msa0837500 MtrunA17_Chr4g0059201 43.243 74 42 0 274 347 320 393 3.07e-16 83.2
Msa0837500 MtrunA17_Chr1g0198984 50.794 63 31 0 286 348 1 63 3.64e-16 75.1
Msa0837500 MtrunA17_Chr3g0117851 42.500 80 46 0 274 353 276 355 1.21e-15 81.3
Msa0837500 MtrunA17_Chr4g0044631 40.000 100 55 3 274 369 15 113 1.44e-15 79.7
Msa0837500 MtrunA17_Chr1g0177261 41.509 106 48 6 265 359 1 103 5.23e-14 75.1
Msa0837500 MtrunA17_Chr3g0110121 38.372 86 49 2 269 350 32 117 5.37e-14 75.1
Msa0837500 MtrunA17_Chr6g0449461 28.866 291 186 10 446 723 10 292 7.52e-14 73.6
Msa0837500 MtrunA17_Chr5g0420471 38.095 84 48 2 271 350 23 106 9.17e-14 74.3
Msa0837500 MtrunA17_Chr5g0434171 39.286 84 47 2 271 350 33 116 1.55e-13 73.6
Msa0837500 MtrunA17_Chr6g0479261 38.095 84 48 2 271 350 23 106 1.76e-13 73.6
Msa0837500 MtrunA17_Chr1g0177281 41.509 106 48 6 265 359 1 103 1.94e-13 73.2
Msa0837500 MtrunA17_Chr1g0177271 45.349 86 37 4 265 343 1 83 4.07e-13 72.4
Msa0837500 MtrunA17_Chr4g0048811 47.761 67 34 1 274 339 6 72 6.95e-13 70.5
Msa0837500 MtrunA17_Chr8g0390311 34.615 104 66 2 246 348 369 471 1.21e-12 72.0
Msa0837500 MtrunA17_Chr5g0429231 25.532 282 195 6 440 712 40 315 1.38e-12 70.1
Msa0837500 MtrunA17_Chr2g0290151 28.743 167 112 5 550 713 290 452 5.25e-12 68.9
Msa0837500 MtrunA17_Chr1g0177301 42.045 88 45 4 269 350 2 89 7.47e-12 68.2
Msa0837500 MtrunA17_Chr5g0435041 24.528 212 114 3 546 712 89 299 1.03e-11 67.4
Msa0837500 MtrunA17_Chr7g0267501 41.573 89 45 4 269 350 4 92 2.62e-11 66.6
Msa0837500 MtrunA17_Chr3g0129811 39.130 69 42 0 271 339 81 149 3.76e-11 66.2
Msa0837500 MtrunA17_Chr2g0280671 44.615 65 36 0 275 339 17 81 6.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0837500 AT4G36550.1 36.592 757 400 17 17 755 12 706 2.04e-138 425
Msa0837500 AT1G24330.1 33.102 719 430 15 19 713 17 708 5.99e-113 360
Msa0837500 AT1G24330.2 33.102 719 430 15 19 713 17 708 5.99e-113 360
Msa0837500 AT1G67530.2 32.708 746 460 16 19 748 17 736 1.59e-110 354
Msa0837500 AT1G67530.1 32.708 746 460 16 19 748 17 736 1.59e-110 354
Msa0837500 AT1G67530.4 32.708 746 460 16 19 748 17 736 1.59e-110 354
Msa0837500 AT1G27910.1 30.590 729 433 15 19 713 17 706 3.68e-91 303
Msa0837500 AT1G67530.3 34.990 503 300 10 19 510 17 503 1.89e-85 281
Msa0837500 AT5G67340.1 25.427 468 307 12 274 710 241 697 3.32e-34 140
Msa0837500 AT5G67340.2 25.427 468 307 12 274 710 241 697 3.32e-34 140
Msa0837500 AT3G46510.1 26.807 332 210 7 23 350 31 333 2.29e-25 112
Msa0837500 AT3G07360.1 53.333 75 35 0 271 345 72 146 6.28e-25 109
Msa0837500 AT5G42340.1 26.061 330 197 9 27 349 77 366 5.55e-24 108
Msa0837500 AT2G28830.1 48.276 87 45 0 262 348 173 259 1.83e-22 102
Msa0837500 AT2G23140.2 46.591 88 47 0 267 354 224 311 4.46e-22 102
Msa0837500 AT2G23140.2 24.900 249 163 6 466 713 594 819 1.19e-12 72.0
Msa0837500 AT2G23140.4 46.591 88 47 0 267 354 224 311 4.46e-22 102
Msa0837500 AT2G23140.4 24.900 249 163 6 466 713 594 819 1.19e-12 72.0
Msa0837500 AT2G23140.1 46.591 88 47 0 267 354 227 314 4.55e-22 102
Msa0837500 AT2G23140.1 24.900 249 163 6 466 713 597 822 1.28e-12 72.0
Msa0837500 AT2G23140.3 46.591 88 47 0 267 354 35 122 4.59e-22 102
Msa0837500 AT2G23140.3 24.900 249 163 6 466 713 405 630 8.43e-13 72.4
Msa0837500 AT2G23140.5 46.591 88 47 0 267 354 35 122 4.59e-22 102
Msa0837500 AT2G23140.5 24.900 249 163 6 466 713 405 630 8.43e-13 72.4
Msa0837500 AT1G23030.1 25.291 344 234 8 259 584 227 565 5.56e-22 101
Msa0837500 AT3G54850.1 52.000 75 36 0 274 348 249 323 1.68e-21 100
Msa0837500 AT1G71020.2 43.000 100 53 1 249 348 75 170 7.91e-21 97.1
Msa0837500 AT1G71020.1 43.000 100 53 1 249 348 223 318 1.94e-20 96.7
Msa0837500 AT3G54790.2 38.372 86 53 0 271 356 200 285 1.36e-17 87.8
Msa0837500 AT3G54790.3 38.372 86 53 0 271 356 236 321 1.84e-17 87.4
Msa0837500 AT3G54790.1 38.372 86 53 0 271 356 236 321 1.84e-17 87.4
Msa0837500 AT1G29340.1 46.053 76 41 0 271 346 303 378 4.23e-16 83.2
Msa0837500 AT5G01830.1 44.444 72 40 0 274 345 275 346 6.60e-16 82.4
Msa0837500 AT5G18340.1 31.757 148 88 2 223 358 12 158 9.77e-16 80.9
Msa0837500 AT1G49780.1 46.914 81 39 2 274 350 15 95 1.55e-15 80.1
Msa0837500 AT5G18320.1 46.575 73 38 1 271 343 70 141 7.84e-15 78.2
Msa0837500 AT3G19380.1 43.902 82 41 3 274 350 15 96 4.58e-14 75.5
Msa0837500 AT1G66160.1 39.130 92 52 2 263 350 23 114 7.13e-14 75.1
Msa0837500 AT5G37490.1 42.045 88 46 2 267 350 26 112 1.05e-13 74.7
Msa0837500 AT2G35930.1 45.122 82 40 3 274 350 13 94 9.64e-13 71.2
Msa0837500 AT3G52450.1 43.902 82 41 3 274 350 8 89 1.38e-12 70.9
Msa0837500 AT1G60190.1 40.244 82 49 0 275 356 280 361 1.50e-12 71.6
Msa0837500 AT1G10560.1 34.906 106 68 1 248 352 262 367 1.61e-12 71.6
Msa0837500 AT1G01680.1 40.244 82 49 0 262 343 222 303 2.68e-12 68.9
Msa0837500 AT5G18330.1 38.571 70 42 1 271 340 63 131 7.58e-12 68.9
Msa0837500 AT3G61390.4 36.842 76 48 0 264 339 269 344 8.24e-12 68.2
Msa0837500 AT3G61390.2 36.842 76 48 0 264 339 344 419 1.54e-11 67.8
Msa0837500 AT3G49810.1 46.970 66 35 0 274 339 65 130 2.28e-11 67.4
Msa0837500 AT1G56040.1 38.961 77 47 0 262 338 342 418 3.11e-11 66.6
Msa0837500 AT5G57035.1 43.939 66 37 0 274 339 709 774 6.07e-11 66.6

Find 165 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAATACCCTCTCCTTTC+AGG 0.129941 5_4:+11494359 Msa0837500:CDS
ATACATCTGCTGCTTAATTT+TGG 0.151569 5_4:+11497864 Msa0837500:three_prime_UTR
TGCATTGGATGCTGCAATTT+TGG 0.187482 5_4:-11496980 None:intergenic
GGATTATCTTAAGTTGATTT+TGG 0.208826 5_4:+11497344 Msa0837500:CDS
AAGTCCTTCATGACAATATT+TGG 0.222378 5_4:-11495882 None:intergenic
ATAACTTCCCAAACTCTTAA+TGG 0.232924 5_4:-11495997 None:intergenic
GCCATAATGGAAGAACTTTC+TGG 0.238374 5_4:+11496940 Msa0837500:CDS
TTGGAAGTTGCTTGCATATT+TGG 0.240940 5_4:+11493600 Msa0837500:five_prime_UTR
TTTCAAGCTCATGAGATTCT+TGG 0.241484 5_4:-11493445 None:intergenic
GTAGAACTGTTGTTGGTAAT+TGG 0.247713 5_4:-11497498 None:intergenic
ACCTGAAAGGAGAGGGTATT+TGG 0.250821 5_4:-11494360 None:intergenic
ACAAAGCTTAATTCCCATTC+AGG 0.250995 5_4:+11495058 Msa0837500:CDS
GTTCAATGAAATTTGTATAT+TGG 0.252298 5_4:-11493486 None:intergenic
AATCACCTCCATTAAGAGTT+TGG 0.258899 5_4:+11495989 Msa0837500:CDS
CCAATAGCACTTGATTAGTT+TGG 0.266982 5_4:+11493581 Msa0837500:five_prime_UTR
TCCAGAAAGTTCTTCCATTA+TGG 0.272030 5_4:-11496941 None:intergenic
AGATCGTTTCTCGATTAAAA+TGG 0.277574 5_4:-11495433 None:intergenic
CCAAACTAATCAAGTGCTAT+TGG 0.279050 5_4:-11493581 None:intergenic
TGAGTATGCTACATTTGTTC+TGG 0.287071 5_4:+11495275 Msa0837500:CDS
CAATAGTAACGTGTAATTCT+AGG 0.289232 5_4:+11493654 Msa0837500:five_prime_UTR
AAGAATAAACAAAATAAAAT+AGG 0.289390 5_4:-11498040 None:intergenic
GATCCTGTTATCATTGCTTC+TGG 0.293374 5_4:+11495762 Msa0837500:CDS
TTCTTCAATAGTAGTCTAAT+TGG 0.304543 5_4:+11493626 Msa0837500:five_prime_UTR
TAAGAGCCAGTTTGGATTGA+TGG 0.313296 5_4:+11497711 Msa0837500:three_prime_UTR
TGTGAATGTGTGTGATATAT+TGG 0.316428 5_4:+11497772 Msa0837500:three_prime_UTR
TACATCAACCTTGAGGATAT+TGG 0.317123 5_4:-11495738 None:intergenic
CAACATGTCACTTGGATCAT+TGG 0.319176 5_4:+11496052 Msa0837500:CDS
ATGCTTGATAAGAGCCAGTT+TGG 0.336886 5_4:+11497703 Msa0837500:three_prime_UTR
ATCACCTCCATTAAGAGTTT+GGG 0.353633 5_4:+11495990 Msa0837500:CDS
CTCAACTTCATGATCTTCAA+TGG 0.371229 5_4:+11496216 Msa0837500:CDS
CACTATTGGTTCTCATTAAG+AGG 0.380327 5_4:-11494317 None:intergenic
CTTCTTGATTCAGAAGTGAT+AGG 0.387240 5_4:+11496907 Msa0837500:CDS
AATCCTAGTTTCTTCAGAAA+TGG 0.387275 5_4:-11497567 None:intergenic
ACTTATGCATCCCTTTGTTT+CGG 0.390748 5_4:-11497229 None:intergenic
AGGAGAAGTACTTGCCATAA+TGG 0.393561 5_4:+11496927 Msa0837500:CDS
TTCCGACAGAAGCTGAGTTC+CGG 0.397671 5_4:-11496390 None:intergenic
GACTGGAACCATACCCTGAA+TGG 0.398503 5_4:-11495072 None:intergenic
CTTAATTCCCATTCAGGGTA+TGG 0.404422 5_4:+11495064 Msa0837500:CDS
TCACAGAATGGAAATGATAA+AGG 0.407952 5_4:+11497399 Msa0837500:CDS
TTATTCTTATAGCATGTTGT+TGG 0.411310 5_4:-11497047 None:intergenic
AACAAAATAAAATAGGAAAC+TGG 0.414555 5_4:-11498033 None:intergenic
TAGCAGATATGATTTATATA+TGG 0.425285 5_4:+11497735 Msa0837500:three_prime_UTR
CTAGAGTTATCCCTTGCAAT+TGG 0.428602 5_4:-11495600 None:intergenic
ACTCCAGAAGCAATGATAAC+AGG 0.429341 5_4:-11495765 None:intergenic
TTTAGTAATATTCTCAAGAA+AGG 0.431108 5_4:-11497800 None:intergenic
TGCTATCCATCAATCCAAAC+TGG 0.436944 5_4:-11497717 None:intergenic
TGTTGGCAGGAAGAGGGAAA+AGG 0.439214 5_4:-11498001 None:intergenic
TGAGATGTTATATTCAGTCT+TGG 0.439810 5_4:-11495405 None:intergenic
TCCAGTCATGTTGGCTGTAG+AGG 0.442914 5_4:+11495088 Msa0837500:CDS
TGAAGATACGTTCATAAAGT+TGG 0.444353 5_4:+11496879 Msa0837500:CDS
TATGTGATGGAAGGGTTTCA+AGG 0.449063 5_4:-11493284 None:intergenic
CATAGAATTGATGAAAATAG+TGG 0.450941 5_4:+11494615 Msa0837500:CDS
ACCTCTACAGCCAACATGAC+TGG 0.454239 5_4:-11495089 None:intergenic
AAGAAGTTTCTAGATAAAGT+TGG 0.465531 5_4:+11495459 Msa0837500:CDS
GGACGTGCTTCCTCTATGTC+TGG 0.467090 5_4:-11494658 None:intergenic
TCAACTTCATGATCTTCAAT+GGG 0.469345 5_4:+11496217 Msa0837500:CDS
TCTGTATGTCTACCTGGATC+AGG 0.469637 5_4:-11495942 None:intergenic
CATCAGATTCAAGTCATGAT+TGG 0.470843 5_4:-11496111 None:intergenic
ATTACTTCTTCAACATTGCT+CGG 0.472225 5_4:+11494741 Msa0837500:CDS
GTTGGCAGGAAGAGGGAAAA+GGG 0.474180 5_4:-11498000 None:intergenic
TATTACTTTCAGGCTGTGAC+AGG 0.476168 5_4:+11494990 Msa0837500:intron
TTGTATAGTGGTGGTGGTGG+TGG 0.476960 5_4:+11497914 Msa0837500:three_prime_UTR
ATTGGACACTTGTATTCCTC+AGG 0.479427 5_4:-11495720 None:intergenic
ACTGAATATAACATCTCAAA+AGG 0.479795 5_4:+11495410 Msa0837500:CDS
GCACGTCCAAGATGTTCATC+AGG 0.480753 5_4:+11494673 Msa0837500:CDS
CATGGCCATGAAAATTGAAA+AGG 0.486010 5_4:-11493361 None:intergenic
TCTATTCCTGATGAACATCT+TGG 0.488308 5_4:-11494679 None:intergenic
TCACCATTTCTGAAGAAACT+AGG 0.488630 5_4:+11497564 Msa0837500:CDS
GATTCTGCTGAAGAAGAGGC+TGG 0.489454 5_4:+11495297 Msa0837500:CDS
CTTCTTAGTAGTAAAATCAA+TGG 0.490228 5_4:+11493530 Msa0837500:five_prime_UTR
AAAGAATGTGGATAAGAAAG+TGG 0.491198 5_4:+11495796 Msa0837500:CDS
GACGTAAAAGCGCTGAGAGC+CGG 0.495304 5_4:+11496371 Msa0837500:CDS
AATTCTATTGCCGAAACAAA+GGG 0.496586 5_4:+11497219 Msa0837500:CDS
TACTTGATTCTGATAACAAA+GGG 0.497678 5_4:+11497022 Msa0837500:CDS
CTCTTAATGAGAACCAATAG+TGG 0.499914 5_4:+11494318 Msa0837500:exon
GGAATCTGTAGAACTGTTGT+TGG 0.501183 5_4:-11497505 None:intergenic
TCTAGTATGTTGGCAGGAAG+AGG 0.501878 5_4:-11498008 None:intergenic
ATGGAAGAACTTTCTGGAGA+TGG 0.503922 5_4:+11496946 Msa0837500:CDS
TTATGAACGTATCTTCACTT+AGG 0.504024 5_4:-11496873 None:intergenic
GAGCAATGCAAATGATCAGC+AGG 0.504932 5_4:+11496349 Msa0837500:CDS
ATTGTATTGTATAGTGGTGG+TGG 0.506458 5_4:+11497908 Msa0837500:three_prime_UTR
TATCCTTGTGATACCATTGT+GGG 0.506979 5_4:+11497665 Msa0837500:three_prime_UTR
GGTGTCTCCATTCCTGATCC+AGG 0.507047 5_4:+11495930 Msa0837500:CDS
ACTGGAACCATACCCTGAAT+GGG 0.507557 5_4:-11495071 None:intergenic
GTAAGAGAATTGCTTCATCA+AGG 0.507831 5_4:+11495327 Msa0837500:CDS
CGGAATTTGTGAACAACTGC+AGG 0.508751 5_4:+11496408 Msa0837500:CDS
TGGTTAAAGTATGTGATGGA+AGG 0.511760 5_4:-11493293 None:intergenic
GGTTAAAGTATGTGATGGAA+GGG 0.511997 5_4:-11493292 None:intergenic
ATGTACATGTTCTCAAAATG+TGG 0.513586 5_4:+11497323 Msa0837500:CDS
CAAAGCTTAATTCCCATTCA+GGG 0.514357 5_4:+11495059 Msa0837500:CDS
GACTCCAAATATTGTCATGA+AGG 0.514758 5_4:+11495878 Msa0837500:CDS
GCATGGAAACTTAATGTTGG+TGG 0.522527 5_4:+11495539 Msa0837500:CDS
GATAAGAAAGTGGATTAATG+AGG 0.523558 5_4:+11495806 Msa0837500:CDS
ACAGTAGGCACTGTAACAGT+AGG 0.525492 5_4:+11497945 Msa0837500:three_prime_UTR
GAGAATATTTCCAGACATAG+AGG 0.529000 5_4:+11494648 Msa0837500:CDS
CTGGTCTCTAGTATGTTGGC+AGG 0.529952 5_4:-11498014 None:intergenic
GAAGTTGCTTGCATATTTGG+TGG 0.533912 5_4:+11493603 Msa0837500:five_prime_UTR
TTTGGAGTCAATGTCATATG+AGG 0.534604 5_4:-11495864 None:intergenic
AAATCTTCTGTATGTCTACC+TGG 0.534943 5_4:-11495948 None:intergenic
TTTATTTATATGTACCTGAA+AGG 0.536292 5_4:-11494373 None:intergenic
AACATTTGTGATACTGAAGA+AGG 0.537856 5_4:+11497192 Msa0837500:CDS
TGGACAAACTTCACTGTTGT+AGG 0.541725 5_4:-11497082 None:intergenic
GACATGTTGGTAAGATCCAT+TGG 0.542509 5_4:-11496038 None:intergenic
GCATGAACTGTATGCTTGTG+AGG 0.545176 5_4:-11496161 None:intergenic
GTGGTGGTTATGTTTACAGT+AGG 0.546072 5_4:+11497930 Msa0837500:three_prime_UTR
GGAATGCAGCAAACTTTACC+TGG 0.549952 5_4:+11494762 Msa0837500:CDS
CAATGATCTGAACTTGCCAA+TGG 0.550541 5_4:+11496022 Msa0837500:CDS
TTACTTGATTCTGATAACAA+AGG 0.553043 5_4:+11497021 Msa0837500:CDS
AGGTTCAACCTCAGATTCCA+TGG 0.554010 5_4:+11495347 Msa0837500:CDS
CATGGAAACTTAATGTTGGT+GGG 0.555579 5_4:+11495540 Msa0837500:CDS
GATTTCTTCGTCGAGAATCA+CGG 0.555802 5_4:-11495623 None:intergenic
TTTAACTTCAAAGTCGTCCA+TGG 0.559348 5_4:-11495364 None:intergenic
GATCTTACCAACATGTCACT+TGG 0.560709 5_4:+11496044 Msa0837500:CDS
TTCTGTGAGATATAGAAAAG+AGG 0.564373 5_4:-11497384 None:intergenic
GAAACTGGTCTCTAGTATGT+TGG 0.564897 5_4:-11498018 None:intergenic
GGGTATGGTTCCAGTCATGT+TGG 0.567915 5_4:+11495079 Msa0837500:CDS
AAATTCTATTGCCGAAACAA+AGG 0.571416 5_4:+11497218 Msa0837500:CDS
CAATGATCCAAGTGACATGT+TGG 0.572790 5_4:-11496051 None:intergenic
TCTGGATTCTGCTGAAGAAG+AGG 0.573607 5_4:+11495293 Msa0837500:CDS
TTATTGGTTAAAGTATGTGA+TGG 0.574426 5_4:-11493297 None:intergenic
ATTGTAATTGTATTGTATAG+TGG 0.575715 5_4:+11497902 Msa0837500:three_prime_UTR
TAAGACAGAAGTGAGTGCAT+TGG 0.577623 5_4:-11496995 None:intergenic
TAGTGAAAATAAAGCAAGCA+TGG 0.578421 5_4:-11493379 None:intergenic
ATGTCTACCTGGATCAGGAA+TGG 0.578674 5_4:-11495937 None:intergenic
AGCCGGAACTCAGCTTCTGT+CGG 0.579942 5_4:+11496388 Msa0837500:CDS
ATTGCTGAAATACTTGAGAG+TGG 0.582850 5_4:+11497255 Msa0837500:CDS
GGACACTTGTATTCCTCAGG+TGG 0.587812 5_4:-11495717 None:intergenic
GTATTGTATAGTGGTGGTGG+TGG 0.590239 5_4:+11497911 Msa0837500:three_prime_UTR
ACACACATTCACAAAGATTG+AGG 0.593009 5_4:-11497762 None:intergenic
CTAGTATGTTGGCAGGAAGA+GGG 0.593142 5_4:-11498007 None:intergenic
CAGTAGGCACTGTAACAGTA+GGG 0.595779 5_4:+11497946 Msa0837500:three_prime_UTR
CTGACACTGACAAAGTAGCT+TGG 0.600806 5_4:-11496292 None:intergenic
TATATGTACCTGAAAGGAGA+GGG 0.600973 5_4:-11494367 None:intergenic
ATTCTGCTGAAGAAGAGGCT+GGG 0.601067 5_4:+11495298 Msa0837500:CDS
GAGTAACATATGAAAGAATG+TGG 0.601967 5_4:+11495784 Msa0837500:CDS
ATAAGAAAGTGGATTAATGA+GGG 0.602588 5_4:+11495807 Msa0837500:CDS
CAGTGAAGTTTGTCCACACA+TGG 0.607997 5_4:+11497089 Msa0837500:CDS
AATGTTGGTGGGAGAACACA+TGG 0.609389 5_4:+11495551 Msa0837500:CDS
GAATGGAAATGATAAAGGAA+AGG 0.610121 5_4:+11497404 Msa0837500:CDS
GATGAATCAGTCGGATAGAG+TGG 0.610590 5_4:+11495692 Msa0837500:CDS
AATGTGTTGAGAATGAAGAT+AGG 0.612607 5_4:+11497466 Msa0837500:CDS
TCCTTGTGATACCATTGTGG+GGG 0.616090 5_4:+11497667 Msa0837500:three_prime_UTR
CAAAATATGAAGCAATTCCA+CGG 0.616213 5_4:-11497433 None:intergenic
TAGAGAAGAACCAATTGCAA+GGG 0.618427 5_4:+11495590 Msa0837500:CDS
CATTGAAGATCATGAAGTTG+AGG 0.619411 5_4:-11496215 None:intergenic
TAAAACTGAGATGAATCAGT+CGG 0.620886 5_4:+11495683 Msa0837500:CDS
GGTCTTAACTTGTGACACAT+CGG 0.622908 5_4:-11496090 None:intergenic
AAAGCATGGAAACTTAATGT+TGG 0.624675 5_4:+11495536 Msa0837500:CDS
GTAGAGAAGAACCAATTGCA+AGG 0.624828 5_4:+11495589 Msa0837500:CDS
AAAGTCGTCCATGGAATCTG+AGG 0.626252 5_4:-11495355 None:intergenic
TTATATGTACCTGAAAGGAG+AGG 0.631111 5_4:-11494368 None:intergenic
ACAAGTGTCCAATATCCTCA+AGG 0.631386 5_4:+11495730 Msa0837500:CDS
ATATCCTTGTGATACCATTG+TGG 0.633299 5_4:+11497664 Msa0837500:three_prime_UTR
GGATAGAGTGGCACCACCTG+AGG 0.636060 5_4:+11495704 Msa0837500:CDS
GTAATTGTATTGTATAGTGG+TGG 0.647763 5_4:+11497905 Msa0837500:three_prime_UTR
ACTTCCCAAACTCTTAATGG+AGG 0.650645 5_4:-11495994 None:intergenic
ATCCTTGTGATACCATTGTG+GGG 0.650807 5_4:+11497666 Msa0837500:three_prime_UTR
TAAAGGAAAGGAAAGTGCCG+TGG 0.657866 5_4:+11497416 Msa0837500:CDS
CACATGCATCAAAACCCAGA+AGG 0.661406 5_4:+11497607 Msa0837500:CDS
CCTTGTGATACCATTGTGGG+GGG 0.667814 5_4:+11497668 Msa0837500:three_prime_UTR
TGAAGGACTTGATATCAAAG+TGG 0.687601 5_4:+11495895 Msa0837500:CDS
AGGATCATACATCAACCTTG+AGG 0.687795 5_4:-11495745 None:intergenic
CAATTGACAGATACCATGTG+TGG 0.700444 5_4:-11497102 None:intergenic
GTTGTTGGTAATTGGCTGAG+AGG 0.715093 5_4:-11497490 None:intergenic
ATTATGACTTGAGAATGCTG+AGG 0.735899 5_4:-11497643 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATAAACAAAATAAAAT+AGG - chr5_4:11498043-11498062 None:intergenic 10.0%
!! GAACAAAAGAAATAAAAAAA+AGG - chr5_4:11493426-11493445 None:intergenic 15.0%
!!! GTTTCTATTATTTTATCTTT+AGG + chr5_4:11493702-11493721 Msa0837500:intron 15.0%
!! ACGATACATAATATATATAA+TGG + chr5_4:11494923-11494942 Msa0837500:intron 15.0%
!!! TTTGTTTTAATATTACTTTC+AGG + chr5_4:11494980-11494999 Msa0837500:intron 15.0%
!! ACAATTGAAAATAGTTTATA+TGG - chr5_4:11496504-11496523 None:intergenic 15.0%
!! TATAAATAATTAAGTTCCTA+AGG + chr5_4:11496640-11496659 Msa0837500:intron 15.0%
!!! GAAAAAATAAGAGGTATTAT+TGG - chr5_4:11493316-11493335 None:intergenic 20.0%
!!! ATTATCCTTTTCAATTTTCA+TGG + chr5_4:11493356-11493375 Msa0837500:five_prime_UTR 20.0%
!! GTTCAATGAAATTTGTATAT+TGG - chr5_4:11493489-11493508 None:intergenic 20.0%
!!! TGTAACTTTTGTCAAAATTA+CGG + chr5_4:11493939-11493958 Msa0837500:intron 20.0%
!!! AATTTATTTTGGAATACTCT+TGG - chr5_4:11494188-11494207 None:intergenic 20.0%
!! TTTATTTATATGTACCTGAA+AGG - chr5_4:11494376-11494395 None:intergenic 20.0%
!! AACAAAATAATGAAATGCAA+TGG - chr5_4:11494401-11494420 None:intergenic 20.0%
!!! TATTTTGTTCATCATATCAT+AGG + chr5_4:11494412-11494431 Msa0837500:intron 20.0%
!!! ATATAGTTTGTTAGTTAGAT+TGG - chr5_4:11494471-11494490 None:intergenic 20.0%
!! TTACATAAACTCTTATAACT+TGG + chr5_4:11494528-11494547 Msa0837500:intron 20.0%
!! AATGGTTTATTTATACTAAC+AGG + chr5_4:11494941-11494960 Msa0837500:intron 20.0%
!! TATATGTCCAAAAACAAAAA+AGG + chr5_4:11495836-11495855 Msa0837500:CDS 20.0%
!!! ACTATTTTCAATTGTAAATG+TGG + chr5_4:11496509-11496528 Msa0837500:intron 20.0%
!! AAATTTATAATACCAAAAGC+AGG - chr5_4:11496808-11496827 None:intergenic 20.0%
!!! TGAAAAAAGGTAACAATTTT+GGG - chr5_4:11497131-11497150 None:intergenic 20.0%
!!! TTGAAAAAAGGTAACAATTT+TGG - chr5_4:11497132-11497151 None:intergenic 20.0%
!! TAGCAGATATGATTTATATA+TGG + chr5_4:11497735-11497754 Msa0837500:three_prime_UTR 20.0%
!! TTTAGTAATATTCTCAAGAA+AGG - chr5_4:11497803-11497822 None:intergenic 20.0%
!! ATTGTAATTGTATTGTATAG+TGG + chr5_4:11497902-11497921 Msa0837500:three_prime_UTR 20.0%
!! AACAAAATAAAATAGGAAAC+TGG - chr5_4:11498036-11498055 None:intergenic 20.0%
!!! GATATCTGTTTTTACAATTA+AGG - chr5_4:11498100-11498119 None:intergenic 20.0%
!! TTATTGGTTAAAGTATGTGA+TGG - chr5_4:11493300-11493319 None:intergenic 25.0%
! TGTAGTCATGAAAAAATAAG+AGG - chr5_4:11493325-11493344 None:intergenic 25.0%
! CTTCTTAGTAGTAAAATCAA+TGG + chr5_4:11493530-11493549 Msa0837500:five_prime_UTR 25.0%
! TTCTTCAATAGTAGTCTAAT+TGG + chr5_4:11493626-11493645 Msa0837500:five_prime_UTR 25.0%
!!! AATAGAAACTTTTGAGAGTA+AGG - chr5_4:11493691-11493710 None:intergenic 25.0%
!!! ATCAAAGTTGATAAATTCAG+AGG - chr5_4:11493813-11493832 None:intergenic 25.0%
! ATCAACTTTGATCAATCAAA+TGG + chr5_4:11493821-11493840 Msa0837500:intron 25.0%
!! CAAATGGCTTACAATTTTAT+TGG + chr5_4:11493837-11493856 Msa0837500:intron 25.0%
! TAGTGAGTTTGACAAAATTT+AGG + chr5_4:11493886-11493905 Msa0837500:intron 25.0%
!! TGATAATTTGCCATATATCT+TGG - chr5_4:11494011-11494030 None:intergenic 25.0%
!! AGAGAAGGACAAATTTATTT+TGG - chr5_4:11494199-11494218 None:intergenic 25.0%
! TTGTTCTTTCATTACAGTTA+AGG + chr5_4:11494227-11494246 Msa0837500:intron 25.0%
! CGGAATTTATGTTAATTAAC+AGG + chr5_4:11494576-11494595 Msa0837500:intron 25.0%
! CATAGAATTGATGAAAATAG+TGG + chr5_4:11494615-11494634 Msa0837500:CDS 25.0%
! AAAAAAAAAAAAGACACACC+AGG - chr5_4:11494783-11494802 None:intergenic 25.0%
!!! AGTTTGGATTAACTTGTTTA+TGG - chr5_4:11495209-11495228 None:intergenic 25.0%
! ACTGAATATAACATCTCAAA+AGG + chr5_4:11495410-11495429 Msa0837500:CDS 25.0%
!! AAGAAGTTTCTAGATAAAGT+TGG + chr5_4:11495459-11495478 Msa0837500:CDS 25.0%
! ATGTATCTAATGAAAAAGCA+TGG + chr5_4:11495522-11495541 Msa0837500:CDS 25.0%
!!! ATAAGAAAGTGGATTAATGA+GGG + chr5_4:11495807-11495826 Msa0837500:CDS 25.0%
!! TACAGAAGATTTTATGTTGT+TGG + chr5_4:11495959-11495978 Msa0837500:CDS 25.0%
! ACCATACAAAGATCAAAATT+GGG - chr5_4:11496454-11496473 None:intergenic 25.0%
!! TCTACAATTTCAGAAACTTT+TGG - chr5_4:11496595-11496614 None:intergenic 25.0%
!!! AAGTTTCTGAAATTGTAGAA+AGG + chr5_4:11496596-11496615 Msa0837500:intron 25.0%
!!! TGTGTGTAATTTTTCAGAAA+TGG + chr5_4:11496841-11496860 Msa0837500:intron 25.0%
!!! GTAATTTTTCAGAAATGGTA+TGG + chr5_4:11496846-11496865 Msa0837500:intron 25.0%
! TTACTTGATTCTGATAACAA+AGG + chr5_4:11497021-11497040 Msa0837500:CDS 25.0%
! TACTTGATTCTGATAACAAA+GGG + chr5_4:11497022-11497041 Msa0837500:CDS 25.0%
! TTATTCTTATAGCATGTTGT+TGG - chr5_4:11497050-11497069 None:intergenic 25.0%
!!! GGATTATCTTAAGTTGATTT+TGG + chr5_4:11497344-11497363 Msa0837500:CDS 25.0%
!!! TTTGATGATCTATTTTCTTC+AGG - chr5_4:11497540-11497559 None:intergenic 25.0%
TAGTGAAAATAAAGCAAGCA+TGG - chr5_4:11493382-11493401 None:intergenic 30.0%
! GATTTTACTACTAAGAAGCA+AGG - chr5_4:11493528-11493547 None:intergenic 30.0%
CAATAGTAACGTGTAATTCT+AGG + chr5_4:11493654-11493673 Msa0837500:five_prime_UTR 30.0%
TTCAAAGTTCAAACTGTGTT+TGG + chr5_4:11493725-11493744 Msa0837500:intron 30.0%
TCAGAGGTTAACAATTTGAT+TGG - chr5_4:11493797-11493816 None:intergenic 30.0%
TTGTAGCTTCAACAAAATCA+TGG - chr5_4:11493917-11493936 None:intergenic 30.0%
! AACTTTTGTCAAAATTACGG+TGG + chr5_4:11493942-11493961 Msa0837500:intron 30.0%
TTGGTACATGTTTGAATTGA+CGG - chr5_4:11493992-11494011 None:intergenic 30.0%
CAAACATGTACCAAGATATA+TGG + chr5_4:11493998-11494017 Msa0837500:intron 30.0%
AGAACAAAATCTACTAGAGA+AGG - chr5_4:11494214-11494233 None:intergenic 30.0%
TATGTGAATGAACGACAAAT+CGG - chr5_4:11494505-11494524 None:intergenic 30.0%
TAACTTGGATGTTAGTACTA+AGG + chr5_4:11494543-11494562 Msa0837500:intron 30.0%
AGTACTAAGGATTCTATTGA+CGG + chr5_4:11494556-11494575 Msa0837500:intron 30.0%
ATTACTTCTTCAACATTGCT+CGG + chr5_4:11494741-11494760 Msa0837500:CDS 30.0%
!!! CTAATGACTTTTTCGCTTTT+TGG - chr5_4:11495037-11495056 None:intergenic 30.0%
AGAAAGGATAAAACTCAGTT+TGG - chr5_4:11495225-11495244 None:intergenic 30.0%
TGAGATGTTATATTCAGTCT+TGG - chr5_4:11495408-11495427 None:intergenic 30.0%
AGATCGTTTCTCGATTAAAA+TGG - chr5_4:11495436-11495455 None:intergenic 30.0%
! TCAAGATTGTCTTCTTTTTC+GGG - chr5_4:11495494-11495513 None:intergenic 30.0%
! CTCAAGATTGTCTTCTTTTT+CGG - chr5_4:11495495-11495514 None:intergenic 30.0%
AAAGCATGGAAACTTAATGT+TGG + chr5_4:11495536-11495555 Msa0837500:CDS 30.0%
TAAAACTGAGATGAATCAGT+CGG + chr5_4:11495683-11495702 Msa0837500:CDS 30.0%
GAGTAACATATGAAAGAATG+TGG + chr5_4:11495784-11495803 Msa0837500:CDS 30.0%
AAAGAATGTGGATAAGAAAG+TGG + chr5_4:11495796-11495815 Msa0837500:CDS 30.0%
!! GATAAGAAAGTGGATTAATG+AGG + chr5_4:11495806-11495825 Msa0837500:CDS 30.0%
!!! GGTAGTTCCTTTTTTGTTTT+TGG - chr5_4:11495846-11495865 None:intergenic 30.0%
AAGTCCTTCATGACAATATT+TGG - chr5_4:11495885-11495904 None:intergenic 30.0%
TCAAAGTGGTGCAAAAATAA+TGG + chr5_4:11495909-11495928 Msa0837500:CDS 30.0%
ATAACTTCCCAAACTCTTAA+TGG - chr5_4:11496000-11496019 None:intergenic 30.0%
TCAACTTCATGATCTTCAAT+GGG + chr5_4:11496217-11496236 Msa0837500:CDS 30.0%
CACCATACAAAGATCAAAAT+TGG - chr5_4:11496455-11496474 None:intergenic 30.0%
! ACTTTTGGATGATTCACTTT+AGG - chr5_4:11496580-11496599 None:intergenic 30.0%
CAAGAAACTTAAAATTTGCG+TGG - chr5_4:11496744-11496763 None:intergenic 30.0%
! AATCAAGCAAACGAATTTTC+AGG - chr5_4:11496771-11496790 None:intergenic 30.0%
TTATGAACGTATCTTCACTT+AGG - chr5_4:11496876-11496895 None:intergenic 30.0%
TGAAGATACGTTCATAAAGT+TGG + chr5_4:11496879-11496898 Msa0837500:CDS 30.0%
AACATTTGTGATACTGAAGA+AGG + chr5_4:11497192-11497211 Msa0837500:CDS 30.0%
AAATTCTATTGCCGAAACAA+AGG + chr5_4:11497218-11497237 Msa0837500:CDS 30.0%
AATTCTATTGCCGAAACAAA+GGG + chr5_4:11497219-11497238 Msa0837500:CDS 30.0%
ATGTACATGTTCTCAAAATG+TGG + chr5_4:11497323-11497342 Msa0837500:CDS 30.0%
TTCTGTGAGATATAGAAAAG+AGG - chr5_4:11497387-11497406 None:intergenic 30.0%
! CTTTTCTATATCTCACAGAA+TGG + chr5_4:11497387-11497406 Msa0837500:CDS 30.0%
TCACAGAATGGAAATGATAA+AGG + chr5_4:11497399-11497418 Msa0837500:CDS 30.0%
GAATGGAAATGATAAAGGAA+AGG + chr5_4:11497404-11497423 Msa0837500:CDS 30.0%
CAAAATATGAAGCAATTCCA+CGG - chr5_4:11497436-11497455 None:intergenic 30.0%
AATGTGTTGAGAATGAAGAT+AGG + chr5_4:11497466-11497485 Msa0837500:CDS 30.0%
AATCCTAGTTTCTTCAGAAA+TGG - chr5_4:11497570-11497589 None:intergenic 30.0%
! GAAATTCATTTTCACCTTCT+GGG - chr5_4:11497624-11497643 None:intergenic 30.0%
TGTGAATGTGTGTGATATAT+TGG + chr5_4:11497772-11497791 Msa0837500:three_prime_UTR 30.0%
!! ATACATCTGCTGCTTAATTT+TGG + chr5_4:11497864-11497883 Msa0837500:three_prime_UTR 30.0%
GTAATTGTATTGTATAGTGG+TGG + chr5_4:11497905-11497924 Msa0837500:three_prime_UTR 30.0%
! GGTTAAAGTATGTGATGGAA+GGG - chr5_4:11493295-11493314 None:intergenic 35.0%
! TGGTTAAAGTATGTGATGGA+AGG - chr5_4:11493296-11493315 None:intergenic 35.0%
CATGGCCATGAAAATTGAAA+AGG - chr5_4:11493364-11493383 None:intergenic 35.0%
TTTCAAGCTCATGAGATTCT+TGG - chr5_4:11493448-11493467 None:intergenic 35.0%
GCAAGGTGAACAAAAAACAA+CGG - chr5_4:11493511-11493530 None:intergenic 35.0%
CCAAACTAATCAAGTGCTAT+TGG - chr5_4:11493584-11493603 None:intergenic 35.0%
! CCAATAGCACTTGATTAGTT+TGG + chr5_4:11493581-11493600 Msa0837500:five_prime_UTR 35.0%
TTGGAAGTTGCTTGCATATT+TGG + chr5_4:11493600-11493619 Msa0837500:five_prime_UTR 35.0%
GGTTAACAATTTGATTGGAG+AGG - chr5_4:11493792-11493811 None:intergenic 35.0%
GAGAGACTTATAACCATGTT+TGG + chr5_4:11493859-11493878 Msa0837500:intron 35.0%
AACTCACTATGATCCAAACA+TGG - chr5_4:11493875-11493894 None:intergenic 35.0%
CTAAATTGAGAAGTGAGAAG+AGG - chr5_4:11494132-11494151 None:intergenic 35.0%
CTCTTCTCACTTCTCAATTT+AGG + chr5_4:11494130-11494149 Msa0837500:intron 35.0%
!! CACTATTGGTTCTCATTAAG+AGG - chr5_4:11494320-11494339 None:intergenic 35.0%
CTCTTAATGAGAACCAATAG+TGG + chr5_4:11494318-11494337 Msa0837500:exon 35.0%
AACCAATAGTGGTGAAAAAG+TGG + chr5_4:11494329-11494348 Msa0837500:CDS 35.0%
TATATGTACCTGAAAGGAGA+GGG - chr5_4:11494370-11494389 None:intergenic 35.0%
TTATATGTACCTGAAAGGAG+AGG - chr5_4:11494371-11494390 None:intergenic 35.0%
GAGAATATTTCCAGACATAG+AGG + chr5_4:11494648-11494667 Msa0837500:CDS 35.0%
TCTATTCCTGATGAACATCT+TGG - chr5_4:11494682-11494701 None:intergenic 35.0%
TAGCTTTCTGAACAAGTCAA+TGG + chr5_4:11494834-11494853 Msa0837500:intron 35.0%
ACAAAGCTTAATTCCCATTC+AGG + chr5_4:11495058-11495077 Msa0837500:CDS 35.0%
CAAAGCTTAATTCCCATTCA+GGG + chr5_4:11495059-11495078 Msa0837500:CDS 35.0%
!! AGTTTTATCCTTTCTGTTGC+AGG + chr5_4:11495230-11495249 Msa0837500:intron 35.0%
TGAGTATGCTACATTTGTTC+TGG + chr5_4:11495275-11495294 Msa0837500:CDS 35.0%
GTAAGAGAATTGCTTCATCA+AGG + chr5_4:11495327-11495346 Msa0837500:CDS 35.0%
! TTTAACTTCAAAGTCGTCCA+TGG - chr5_4:11495367-11495386 None:intergenic 35.0%
CATGGAAACTTAATGTTGGT+GGG + chr5_4:11495540-11495559 Msa0837500:CDS 35.0%
TAGAGAAGAACCAATTGCAA+GGG + chr5_4:11495590-11495609 Msa0837500:CDS 35.0%
TACATCAACCTTGAGGATAT+TGG - chr5_4:11495741-11495760 None:intergenic 35.0%
! TTTGGAGTCAATGTCATATG+AGG - chr5_4:11495867-11495886 None:intergenic 35.0%
GACTCCAAATATTGTCATGA+AGG + chr5_4:11495878-11495897 Msa0837500:CDS 35.0%
TGAAGGACTTGATATCAAAG+TGG + chr5_4:11495895-11495914 Msa0837500:CDS 35.0%
AAATCTTCTGTATGTCTACC+TGG - chr5_4:11495951-11495970 None:intergenic 35.0%
AATCACCTCCATTAAGAGTT+TGG + chr5_4:11495989-11496008 Msa0837500:CDS 35.0%
ATCACCTCCATTAAGAGTTT+GGG + chr5_4:11495990-11496009 Msa0837500:CDS 35.0%
CATCAGATTCAAGTCATGAT+TGG - chr5_4:11496114-11496133 None:intergenic 35.0%
CATTGAAGATCATGAAGTTG+AGG - chr5_4:11496218-11496237 None:intergenic 35.0%
CTCAACTTCATGATCTTCAA+TGG + chr5_4:11496216-11496235 Msa0837500:CDS 35.0%
TTGCTCAAAAATCTGACTAC+TGG - chr5_4:11496335-11496354 None:intergenic 35.0%
CAAAGATCAAAATTGGGCAA+GGG - chr5_4:11496448-11496467 None:intergenic 35.0%
ACAAAGATCAAAATTGGGCA+AGG - chr5_4:11496449-11496468 None:intergenic 35.0%
!! GCCCAATTTTGATCTTTGTA+TGG + chr5_4:11496450-11496469 Msa0837500:intron 35.0%
ATCCTCAATCATATCCATGT+CGG - chr5_4:11496670-11496689 None:intergenic 35.0%
AAAATTTGCGTGGATCAACT+TGG - chr5_4:11496734-11496753 None:intergenic 35.0%
! TTTATCTTTGCACCTGCTTT+TGG + chr5_4:11496793-11496812 Msa0837500:intron 35.0%
CTTCTTGATTCAGAAGTGAT+AGG + chr5_4:11496907-11496926 Msa0837500:CDS 35.0%
TCCAGAAAGTTCTTCCATTA+TGG - chr5_4:11496944-11496963 None:intergenic 35.0%
! ACAGCTCTGTCTTTGAAAAA+AGG - chr5_4:11497144-11497163 None:intergenic 35.0%
! ACTTATGCATCCCTTTGTTT+CGG - chr5_4:11497232-11497251 None:intergenic 35.0%
ATTGCTGAAATACTTGAGAG+TGG + chr5_4:11497255-11497274 Msa0837500:CDS 35.0%
!! GTAGAACTGTTGTTGGTAAT+TGG - chr5_4:11497501-11497520 None:intergenic 35.0%
! ATTTTCTTCAGGATGACTCT+TGG - chr5_4:11497529-11497548 None:intergenic 35.0%
! TCACCATTTCTGAAGAAACT+AGG + chr5_4:11497564-11497583 Msa0837500:CDS 35.0%
! GGAAATTCATTTTCACCTTC+TGG - chr5_4:11497625-11497644 None:intergenic 35.0%
ATTATGACTTGAGAATGCTG+AGG - chr5_4:11497646-11497665 None:intergenic 35.0%
ATATCCTTGTGATACCATTG+TGG + chr5_4:11497664-11497683 Msa0837500:three_prime_UTR 35.0%
TATCCTTGTGATACCATTGT+GGG + chr5_4:11497665-11497684 Msa0837500:three_prime_UTR 35.0%
ACACACATTCACAAAGATTG+AGG - chr5_4:11497765-11497784 None:intergenic 35.0%
! TAATTTTGGACTGTAACAGC+AGG + chr5_4:11497878-11497897 Msa0837500:three_prime_UTR 35.0%
ATTGTATTGTATAGTGGTGG+TGG + chr5_4:11497908-11497927 Msa0837500:three_prime_UTR 35.0%
GAAGTTGCTTGCATATTTGG+TGG + chr5_4:11493603-11493622 Msa0837500:five_prime_UTR 40.0%
CGGTGAGTTTAACAGAATCA+CGG - chr5_4:11493972-11493991 None:intergenic 40.0%
CTACAGCTACATATCAGACA+AGG - chr5_4:11494291-11494310 None:intergenic 40.0%
CTTGTCTGATATGTAGCTGT+AGG + chr5_4:11494289-11494308 Msa0837500:intron 40.0%
!!! CTCCACTTTTTCACCACTAT+TGG - chr5_4:11494334-11494353 None:intergenic 40.0%
GTTTCGTAATCAAGCTTACG+AGG + chr5_4:11494869-11494888 Msa0837500:intron 40.0%
TATTACTTTCAGGCTGTGAC+AGG + chr5_4:11494990-11495009 Msa0837500:intron 40.0%
CTTAATTCCCATTCAGGGTA+TGG + chr5_4:11495064-11495083 Msa0837500:CDS 40.0%
TCTAGAAACCTGCAACAGAA+AGG - chr5_4:11495241-11495260 None:intergenic 40.0%
!!! GTCTTCTTTTTCGGGTCATT+TGG - chr5_4:11495486-11495505 None:intergenic 40.0%
GCATGGAAACTTAATGTTGG+TGG + chr5_4:11495539-11495558 Msa0837500:CDS 40.0%
GTAGAGAAGAACCAATTGCA+AGG + chr5_4:11495589-11495608 Msa0837500:CDS 40.0%
CTAGAGTTATCCCTTGCAAT+TGG - chr5_4:11495603-11495622 None:intergenic 40.0%
GATTTCTTCGTCGAGAATCA+CGG - chr5_4:11495626-11495645 None:intergenic 40.0%
ATTGGACACTTGTATTCCTC+AGG - chr5_4:11495723-11495742 None:intergenic 40.0%
ACAAGTGTCCAATATCCTCA+AGG + chr5_4:11495730-11495749 Msa0837500:CDS 40.0%
AGGATCATACATCAACCTTG+AGG - chr5_4:11495748-11495767 None:intergenic 40.0%
GATCCTGTTATCATTGCTTC+TGG + chr5_4:11495762-11495781 Msa0837500:CDS 40.0%
ACTCCAGAAGCAATGATAAC+AGG - chr5_4:11495768-11495787 None:intergenic 40.0%
ACTTCCCAAACTCTTAATGG+AGG - chr5_4:11495997-11496016 None:intergenic 40.0%
CAATGATCTGAACTTGCCAA+TGG + chr5_4:11496022-11496041 Msa0837500:CDS 40.0%
! GACATGTTGGTAAGATCCAT+TGG - chr5_4:11496041-11496060 None:intergenic 40.0%
GATCTTACCAACATGTCACT+TGG + chr5_4:11496044-11496063 Msa0837500:CDS 40.0%
CAATGATCCAAGTGACATGT+TGG - chr5_4:11496054-11496073 None:intergenic 40.0%
! CAACATGTCACTTGGATCAT+TGG + chr5_4:11496052-11496071 Msa0837500:CDS 40.0%
!! GGTCTTAACTTGTGACACAT+CGG - chr5_4:11496093-11496112 None:intergenic 40.0%
!! AGGAGAAGTACTTGCCATAA+TGG + chr5_4:11496927-11496946 Msa0837500:CDS 40.0%
! GCCATAATGGAAGAACTTTC+TGG + chr5_4:11496940-11496959 Msa0837500:CDS 40.0%
! ATGGAAGAACTTTCTGGAGA+TGG + chr5_4:11496946-11496965 Msa0837500:CDS 40.0%
! TGCATTGGATGCTGCAATTT+TGG - chr5_4:11496983-11497002 None:intergenic 40.0%
TAAGACAGAAGTGAGTGCAT+TGG - chr5_4:11496998-11497017 None:intergenic 40.0%
TGGACAAACTTCACTGTTGT+AGG - chr5_4:11497085-11497104 None:intergenic 40.0%
CAATTGACAGATACCATGTG+TGG - chr5_4:11497105-11497124 None:intergenic 40.0%
GGAATCTGTAGAACTGTTGT+TGG - chr5_4:11497508-11497527 None:intergenic 40.0%
ATCCTTGTGATACCATTGTG+GGG + chr5_4:11497666-11497685 Msa0837500:three_prime_UTR 40.0%
ATGCTTGATAAGAGCCAGTT+TGG + chr5_4:11497703-11497722 Msa0837500:three_prime_UTR 40.0%
TAAGAGCCAGTTTGGATTGA+TGG + chr5_4:11497711-11497730 Msa0837500:three_prime_UTR 40.0%
TGCTATCCATCAATCCAAAC+TGG - chr5_4:11497720-11497739 None:intergenic 40.0%
GTGGTGGTTATGTTTACAGT+AGG + chr5_4:11497930-11497949 Msa0837500:three_prime_UTR 40.0%
! GAAACTGGTCTCTAGTATGT+TGG - chr5_4:11498021-11498040 None:intergenic 40.0%
TCCAAATACCCTCTCCTTTC+AGG + chr5_4:11494359-11494378 Msa0837500:CDS 45.0%
!! ACCTGAAAGGAGAGGGTATT+TGG - chr5_4:11494363-11494382 None:intergenic 45.0%
GGAATGCAGCAAACTTTACC+TGG + chr5_4:11494762-11494781 Msa0837500:CDS 45.0%
ACTGGAACCATACCCTGAAT+GGG - chr5_4:11495074-11495093 None:intergenic 45.0%
TCTGGATTCTGCTGAAGAAG+AGG + chr5_4:11495293-11495312 Msa0837500:CDS 45.0%
ATTCTGCTGAAGAAGAGGCT+GGG + chr5_4:11495298-11495317 Msa0837500:CDS 45.0%
AGGTTCAACCTCAGATTCCA+TGG + chr5_4:11495347-11495366 Msa0837500:CDS 45.0%
AAAGTCGTCCATGGAATCTG+AGG - chr5_4:11495358-11495377 None:intergenic 45.0%
! AATGTTGGTGGGAGAACACA+TGG + chr5_4:11495551-11495570 Msa0837500:CDS 45.0%
GATGAATCAGTCGGATAGAG+TGG + chr5_4:11495692-11495711 Msa0837500:CDS 45.0%
ATGTCTACCTGGATCAGGAA+TGG - chr5_4:11495940-11495959 None:intergenic 45.0%
TCTGTATGTCTACCTGGATC+AGG - chr5_4:11495945-11495964 None:intergenic 45.0%
GCATGAACTGTATGCTTGTG+AGG - chr5_4:11496164-11496183 None:intergenic 45.0%
CTGACACTGACAAAGTAGCT+TGG - chr5_4:11496295-11496314 None:intergenic 45.0%
GAGCAATGCAAATGATCAGC+AGG + chr5_4:11496349-11496368 Msa0837500:CDS 45.0%
CGGAATTTGTGAACAACTGC+AGG + chr5_4:11496408-11496427 Msa0837500:CDS 45.0%
TATCCATGTCGGACAACCTT+AGG - chr5_4:11496659-11496678 None:intergenic 45.0%
GTCCGACATGGATATGATTG+AGG + chr5_4:11496665-11496684 Msa0837500:intron 45.0%
CAGTGAAGTTTGTCCACACA+TGG + chr5_4:11497089-11497108 Msa0837500:CDS 45.0%
!! TAAAGGAAAGGAAAGTGCCG+TGG + chr5_4:11497416-11497435 Msa0837500:CDS 45.0%
!! GTTGTTGGTAATTGGCTGAG+AGG - chr5_4:11497493-11497512 None:intergenic 45.0%
!! CTTCTGGGTTTTGATGCATG+TGG - chr5_4:11497609-11497628 None:intergenic 45.0%
CACATGCATCAAAACCCAGA+AGG + chr5_4:11497607-11497626 Msa0837500:CDS 45.0%
TCCTTGTGATACCATTGTGG+GGG + chr5_4:11497667-11497686 Msa0837500:three_prime_UTR 45.0%
GATATGTATTCCCCCCACAA+TGG - chr5_4:11497681-11497700 None:intergenic 45.0%
!! GTATTGTATAGTGGTGGTGG+TGG + chr5_4:11497911-11497930 Msa0837500:three_prime_UTR 45.0%
! ACAGTAGGCACTGTAACAGT+AGG + chr5_4:11497945-11497964 Msa0837500:three_prime_UTR 45.0%
! CAGTAGGCACTGTAACAGTA+GGG + chr5_4:11497946-11497965 Msa0837500:three_prime_UTR 45.0%
CTAGTATGTTGGCAGGAAGA+GGG - chr5_4:11498010-11498029 None:intergenic 45.0%
TCTAGTATGTTGGCAGGAAG+AGG - chr5_4:11498011-11498030 None:intergenic 45.0%
GCACGTCCAAGATGTTCATC+AGG + chr5_4:11494673-11494692 Msa0837500:CDS 50.0%
GACTGGAACCATACCCTGAA+TGG - chr5_4:11495075-11495094 None:intergenic 50.0%
GGGTATGGTTCCAGTCATGT+TGG + chr5_4:11495079-11495098 Msa0837500:CDS 50.0%
! TCCAGTCATGTTGGCTGTAG+AGG + chr5_4:11495088-11495107 Msa0837500:CDS 50.0%
ACCTCTACAGCCAACATGAC+TGG - chr5_4:11495092-11495111 None:intergenic 50.0%
GATTCTGCTGAAGAAGAGGC+TGG + chr5_4:11495297-11495316 Msa0837500:CDS 50.0%
GGACACTTGTATTCCTCAGG+TGG - chr5_4:11495720-11495739 None:intergenic 50.0%
TTCCGACAGAAGCTGAGTTC+CGG - chr5_4:11496393-11496412 None:intergenic 50.0%
GTTCCTAAGGTTGTCCGACA+TGG + chr5_4:11496653-11496672 Msa0837500:intron 50.0%
TGCGTGGATCAACTTGGTTG+CGG - chr5_4:11496728-11496747 None:intergenic 50.0%
CCTTGTGATACCATTGTGGG+GGG + chr5_4:11497668-11497687 Msa0837500:three_prime_UTR 50.0%
!! TTGTATAGTGGTGGTGGTGG+TGG + chr5_4:11497914-11497933 Msa0837500:three_prime_UTR 50.0%
GTTGGCAGGAAGAGGGAAAA+GGG - chr5_4:11498003-11498022 None:intergenic 50.0%
TGTTGGCAGGAAGAGGGAAA+AGG - chr5_4:11498004-11498023 None:intergenic 50.0%
CTGGTCTCTAGTATGTTGGC+AGG - chr5_4:11498017-11498036 None:intergenic 50.0%
GGACGTGCTTCCTCTATGTC+TGG - chr5_4:11494661-11494680 None:intergenic 55.0%
GGTGTCTCCATTCCTGATCC+AGG + chr5_4:11495930-11495949 Msa0837500:CDS 55.0%
!! GACGTAAAAGCGCTGAGAGC+CGG + chr5_4:11496371-11496390 Msa0837500:CDS 55.0%
AGCCGGAACTCAGCTTCTGT+CGG + chr5_4:11496388-11496407 Msa0837500:CDS 55.0%
CCCCCCACAATGGTATCACA+AGG - chr5_4:11497671-11497690 None:intergenic 55.0%
GGATAGAGTGGCACCACCTG+AGG + chr5_4:11495704-11495723 Msa0837500:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 11493287 11498157 11493287 ID=Msa0837500;Name=Msa0837500
chr5_4 mRNA 11493287 11498157 11493287 ID=Msa0837500-mRNA-1;Parent=Msa0837500;Name=Msa0837500-mRNA-1;_AED=0.05;_eAED=0.05;_QI=408|1|1|1|0.8|0.83|6|525|767
chr5_4 exon 11493287 11493675 11493287 ID=Msa0837500-mRNA-1:exon:5017;Parent=Msa0837500-mRNA-1
chr5_4 exon 11494305 11494380 11494305 ID=Msa0837500-mRNA-1:exon:5018;Parent=Msa0837500-mRNA-1
chr5_4 exon 11494598 11494783 11494598 ID=Msa0837500-mRNA-1:exon:5019;Parent=Msa0837500-mRNA-1
chr5_4 exon 11495002 11495109 11495002 ID=Msa0837500-mRNA-1:exon:5020;Parent=Msa0837500-mRNA-1
chr5_4 exon 11495252 11496429 11495252 ID=Msa0837500-mRNA-1:exon:5021;Parent=Msa0837500-mRNA-1
chr5_4 exon 11496858 11498157 11496858 ID=Msa0837500-mRNA-1:exon:5022;Parent=Msa0837500-mRNA-1
chr5_4 five_prime_UTR 11493287 11493675 11493287 ID=Msa0837500-mRNA-1:five_prime_utr;Parent=Msa0837500-mRNA-1
chr5_4 five_prime_UTR 11494305 11494323 11494305 ID=Msa0837500-mRNA-1:five_prime_utr;Parent=Msa0837500-mRNA-1
chr5_4 CDS 11494324 11494380 11494324 ID=Msa0837500-mRNA-1:cds;Parent=Msa0837500-mRNA-1
chr5_4 CDS 11494598 11494783 11494598 ID=Msa0837500-mRNA-1:cds;Parent=Msa0837500-mRNA-1
chr5_4 CDS 11495002 11495109 11495002 ID=Msa0837500-mRNA-1:cds;Parent=Msa0837500-mRNA-1
chr5_4 CDS 11495252 11496429 11495252 ID=Msa0837500-mRNA-1:cds;Parent=Msa0837500-mRNA-1
chr5_4 CDS 11496858 11497632 11496858 ID=Msa0837500-mRNA-1:cds;Parent=Msa0837500-mRNA-1
chr5_4 three_prime_UTR 11497633 11498157 11497633 ID=Msa0837500-mRNA-1:three_prime_utr;Parent=Msa0837500-mRNA-1
Gene Sequence

>Msa0837500

ATGAGAACCAATAGTGGTGAAAAAGTGGAGACTTTTCCAAATACCCTCTCCTTTCAGGTACATAGTAAAATATGCATAGAATTGATGAAAATAGTGGACAGCATCATGAGAATATTTCCAGACATAGAGGAAGCACGTCCAAGATGTTCATCAGGAATAGAGTCTCTATGTTTTCTGAACAACTCAATTGAGAAAGCTAAATTACTTCTTCAACATTGCTCGGAATGCAGCAAACTTTACCTGGCTGTGACAGGAGAAACAGTACTTTCGAGATGCCAAAAAGCGAAAAAGTCATTAGAACAAAGCTTAATTCCCATTCAGGGTATGGTTCCAGTCATGTTGGCTGTAGAGGTTTCTAGAATAATTGATGATCTTGAGTATGCTACATTTGTTCTGGATTCTGCTGAAGAAGAGGCTGGGAGAGTTGTAAGAGAATTGCTTCATCAAGGTTCAACCTCAGATTCCATGGACGACTTTGAAGTTAAAGCTCTTCAGTTTGCAGCACCAAGACTGAATATAACATCTCAAAAGGCCATTTTAATCGAGAAACGATCTATCAAGAAGTTTCTAGATAAAGTTGGACCAAATGACCCGAAAAAGAAGACAATCTTGAGATATCTTATGTATCTAATGAAAAAGCATGGAAACTTAATGTTGGTGGGAGAACACATGGATAATTTTTATTCTCGTAGAGAAGAACCAATTGCAAGGGATAACTCTAGCCGTGATTCTCGACGAAGAAATCGTGTTGAGTCAGATCATAGCATGAACTATGATCAGCATAAAACTGAGATGAATCAGTCGGATAGAGTGGCACCACCTGAGGAATACAAGTGTCCAATATCCTCAAGGTTGATGTATGATCCTGTTATCATTGCTTCTGGAGTAACATATGAAAGAATGTGGATAAGAAAGTGGATTAATGAGGGTAAAACTATATGTCCAAAAACAAAAAAGGAACTACCTCATATGACATTGACTCCAAATATTGTCATGAAGGACTTGATATCAAAGTGGTGCAAAAATAATGGTGTCTCCATTCCTGATCCAGGTAGACATACAGAAGATTTTATGTTGTTGGATGCTTCAATCACCTCCATTAAGAGTTTGGGAAGTTATTTCAATGATCTGAACTTGCCAATGGATCTTACCAACATGTCACTTGGATCATTGGATAATAGTTTTAGTTCCGATGTGTCACAAGTTAAGACCAATCATGACTTGAATCTGATGATGATCAAGAGTAATGAAAATTCTCAGCCTCACAAGCATACAGTTCATGCAGAGATACATGATACAGATTTGATGCTCTTGCCTCAACTTCATGATCTTCAATGGGATTCTCAATGTAAGATCATTGAAGATTTGAAAGATCATATGAAAAGCAATAGCCAAGCTACTTTGTCAGTGTCAGCTGAAAATTTAGTTGAACCAGTAGTCAGATTTTTGAGCAATGCAAATGATCAGCAGGACGTAAAAGCGCTGAGAGCCGGAACTCAGCTTCTGTCGGAATTTGTGAACAACTGCAGAAATGGTATGGCTAACCTAAGTGAAGATACGTTCATAAAGTTGGCAAATCTTCTTGATTCAGAAGTGATAGGAGAAGTACTTGCCATAATGGAAGAACTTTCTGGAGATGGAAATAGTAAAGCCAAAATTGCAGCATCCAATGCACTCACTTCTGTCTTAAAGTTACTTGATTCTGATAACAAAGGGTTCCAACAACATGCTATAAGAATAATCTACAATTTGTCCTACAACAGTGAAGTTTGTCCACACATGGTATCTGTCAATTGCATCCCAAAATTGTTACCTTTTTTCAAAGACAGAGCTGTTTTGAGATACTGCATATATATATTGAAAAACATTTGTGATACTGAAGAAGGTAGAAATTCTATTGCCGAAACAAAGGGATGCATAAGTTCTATTGCTGAAATACTTGAGAGTGGAAGTAATGAAGAGCAAGAACATGCACTTGATGTTCTACTATCTTTATGTACATGTTCTCAAAATGTGGATTATCTTAAGTTGATTTTGGATGAAGATATTATCACTCCTCTTTTCTATATCTCACAGAATGGAAATGATAAAGGAAAGGAAAGTGCCGTGGAATTGCTTCATATTTTGAGAGATGCAAAATGTGTTGAGAATGAAGATAGGTCCTCTCAGCCAATTACCAACAACAGTTCTACAGATTCCAAGAGTCATCCTGAAGAAAATAGATCATCAAAGAAATCACCATTTCTGAAGAAACTAGGATTGTTCTCGAAATCTACTCCACATGCATCAAAACCCAGAAGGTGA
Protein sequence

>Msa0837500

MRTNSGEKVETFPNTLSFQVHSKICIELMKIVDSIMRIFPDIEEARPRCSSGIESLCFLNNSIEKAKLLLQHCSECSKLYLAVTGETVLSRCQKAKKSLEQSLIPIQGMVPVMLAVEVSRIIDDLEYATFVLDSAEEEAGRVVRELLHQGSTSDSMDDFEVKALQFAAPRLNITSQKAILIEKRSIKKFLDKVGPNDPKKKTILRYLMYLMKKHGNLMLVGEHMDNFYSRREEPIARDNSSRDSRRRNRVESDHSMNYDQHKTEMNQSDRVAPPEEYKCPISSRLMYDPVIIASGVTYERMWIRKWINEGKTICPKTKKELPHMTLTPNIVMKDLISKWCKNNGVSIPDPGRHTEDFMLLDASITSIKSLGSYFNDLNLPMDLTNMSLGSLDNSFSSDVSQVKTNHDLNLMMIKSNENSQPHKHTVHAEIHDTDLMLLPQLHDLQWDSQCKIIEDLKDHMKSNSQATLSVSAENLVEPVVRFLSNANDQQDVKALRAGTQLLSEFVNNCRNGMANLSEDTFIKLANLLDSEVIGEVLAIMEELSGDGNSKAKIAASNALTSVLKLLDSDNKGFQQHAIRIIYNLSYNSEVCPHMVSVNCIPKLLPFFKDRAVLRYCIYILKNICDTEEGRNSIAETKGCISSIAEILESGSNEEQEHALDVLLSLCTCSQNVDYLKLILDEDIITPLFYISQNGNDKGKESAVELLHILRDAKCVENEDRSSQPITNNSSTDSKSHPEENRSSKKSPFLKKLGLFSKSTPHASKPRR*