AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0757980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757980 MtrunA17_Chr5g0404311 95.312 768 35 1 1 768 1 767 0.0 1474
Msa0757980 MtrunA17_Chr8g0373031 56.522 759 316 6 1 756 1 748 0.0 845
Msa0757980 MtrunA17_Chr8g0377151 31.198 718 447 13 19 713 19 712 5.92e-102 331
Msa0757980 MtrunA17_Chr3g0122721 30.769 767 483 16 10 754 9 749 1.02e-99 325
Msa0757980 MtrunA17_Chr1g0155261 41.627 209 121 1 19 226 16 224 1.57e-38 143
Msa0757980 MtrunA17_Chr1g0183781 28.257 499 281 17 271 713 239 716 2.29e-30 128
Msa0757980 MtrunA17_Chr7g0237931 26.196 439 250 14 274 709 238 605 8.40e-28 119
Msa0757980 MtrunA17_Chr7g0275081 38.571 140 84 1 225 362 28 167 1.58e-27 116
Msa0757980 MtrunA17_Chr4g0044411 49.412 85 43 0 264 348 264 348 9.28e-24 107
Msa0757980 MtrunA17_Chr2g0284461 21.510 437 288 10 274 710 293 674 2.88e-22 102
Msa0757980 MtrunA17_Chr1g0199321 53.333 75 35 0 274 348 248 322 3.24e-22 102
Msa0757980 MtrunA17_Chr7g0234671 38.261 115 64 1 242 356 54 161 7.74e-22 99.4
Msa0757980 MtrunA17_Chr4g0031431 25.478 314 196 9 41 348 49 330 1.16e-21 100
Msa0757980 MtrunA17_Chr3g0106931 48.889 90 45 1 266 354 225 314 1.82e-21 100
Msa0757980 MtrunA17_Chr1g0189811 39.175 97 59 0 274 370 64 160 1.09e-20 95.9
Msa0757980 MtrunA17_Chr7g0248251 37.903 124 64 2 253 367 258 377 1.92e-20 96.3
Msa0757980 MtrunA17_Chr4g0013091 24.070 457 270 15 266 710 306 697 1.64e-19 93.6
Msa0757980 MtrunA17_Chr7g0261371 47.436 78 41 0 271 348 239 316 2.05e-19 93.6
Msa0757980 MtrunA17_Chr7g0261371 29.655 290 162 12 451 713 516 790 6.40e-12 69.3
Msa0757980 MtrunA17_Chr5g0437411 48.649 74 38 0 274 347 294 367 1.93e-18 90.1
Msa0757980 MtrunA17_Chr5g0413681 45.122 82 45 0 275 356 282 363 6.48e-18 88.6
Msa0757980 MtrunA17_Chr4g0059201 40.964 83 49 0 274 356 320 402 1.35e-16 84.3
Msa0757980 MtrunA17_Chr1g0198984 50.794 63 31 0 286 348 1 63 3.61e-16 75.1
Msa0757980 MtrunA17_Chr3g0117851 42.500 80 46 0 274 353 276 355 9.36e-16 81.3
Msa0757980 MtrunA17_Chr4g0044631 40.000 100 55 3 274 369 15 113 1.48e-15 79.7
Msa0757980 MtrunA17_Chr1g0177261 41.509 106 48 6 265 359 1 103 4.43e-14 75.5
Msa0757980 MtrunA17_Chr3g0110121 38.372 86 49 2 269 350 32 117 5.58e-14 75.1
Msa0757980 MtrunA17_Chr6g0449461 28.866 291 186 10 446 723 10 292 7.54e-14 73.6
Msa0757980 MtrunA17_Chr5g0420471 38.095 84 48 2 271 350 23 106 9.11e-14 74.3
Msa0757980 MtrunA17_Chr5g0434171 39.286 84 47 2 271 350 33 116 1.51e-13 73.6
Msa0757980 MtrunA17_Chr1g0177281 41.509 106 48 6 265 359 1 103 1.63e-13 73.6
Msa0757980 MtrunA17_Chr6g0479261 38.095 84 48 2 271 350 23 106 1.74e-13 73.6
Msa0757980 MtrunA17_Chr1g0177271 45.349 86 37 4 265 343 1 83 4.18e-13 72.4
Msa0757980 MtrunA17_Chr4g0048811 47.761 67 34 1 274 339 6 72 6.96e-13 70.5
Msa0757980 MtrunA17_Chr8g0390311 34.615 104 66 2 246 348 369 471 1.18e-12 72.0
Msa0757980 MtrunA17_Chr5g0429231 25.532 282 195 6 440 712 40 315 1.34e-12 70.1
Msa0757980 MtrunA17_Chr2g0290151 28.743 167 112 5 550 713 290 452 4.99e-12 68.9
Msa0757980 MtrunA17_Chr1g0177301 42.045 88 45 4 269 350 2 89 7.62e-12 68.2
Msa0757980 MtrunA17_Chr5g0435041 24.528 212 114 3 546 712 89 299 1.07e-11 67.4
Msa0757980 MtrunA17_Chr7g0267501 41.573 89 45 4 269 350 4 92 2.63e-11 66.6
Msa0757980 MtrunA17_Chr3g0129811 39.130 69 42 0 271 339 81 149 3.90e-11 66.2
Msa0757980 MtrunA17_Chr2g0280671 44.615 65 36 0 275 339 17 81 7.20e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757980 AT4G36550.1 36.856 757 399 17 17 756 12 706 1.49e-138 426
Msa0757980 AT1G24330.1 33.102 719 430 15 19 713 17 708 4.34e-113 361
Msa0757980 AT1G24330.2 33.102 719 430 15 19 713 17 708 4.34e-113 361
Msa0757980 AT1G67530.2 32.664 747 460 16 19 749 17 736 3.22e-110 353
Msa0757980 AT1G67530.1 32.664 747 460 16 19 749 17 736 3.22e-110 353
Msa0757980 AT1G67530.4 32.664 747 460 16 19 749 17 736 3.22e-110 353
Msa0757980 AT1G27910.1 30.590 729 433 15 19 713 17 706 4.31e-91 302
Msa0757980 AT1G67530.3 34.990 503 300 10 19 510 17 503 1.27e-85 282
Msa0757980 AT5G67340.1 25.427 468 307 12 274 710 241 697 2.27e-34 140
Msa0757980 AT5G67340.2 25.427 468 307 12 274 710 241 697 2.27e-34 140
Msa0757980 AT3G46510.1 26.807 332 210 7 23 350 31 333 3.56e-25 111
Msa0757980 AT3G07360.1 53.333 75 35 0 271 345 72 146 6.24e-25 109
Msa0757980 AT5G42340.1 26.061 330 197 9 27 349 77 366 5.76e-24 108
Msa0757980 AT2G28830.1 48.276 87 45 0 262 348 173 259 1.85e-22 102
Msa0757980 AT2G23140.3 46.591 88 47 0 267 354 35 122 3.57e-22 102
Msa0757980 AT2G23140.3 24.900 249 163 6 466 713 405 630 8.45e-13 72.4
Msa0757980 AT2G23140.5 46.591 88 47 0 267 354 35 122 3.57e-22 102
Msa0757980 AT2G23140.5 24.900 249 163 6 466 713 405 630 8.45e-13 72.4
Msa0757980 AT2G23140.1 46.591 88 47 0 267 354 227 314 3.62e-22 102
Msa0757980 AT2G23140.1 24.900 249 163 6 466 713 597 822 1.23e-12 72.0
Msa0757980 AT1G23030.1 24.709 344 236 7 259 584 227 565 3.82e-22 102
Msa0757980 AT2G23140.2 46.591 88 47 0 267 354 224 311 3.86e-22 102
Msa0757980 AT2G23140.2 24.900 249 163 6 466 713 594 819 1.18e-12 72.0
Msa0757980 AT2G23140.4 46.591 88 47 0 267 354 224 311 3.86e-22 102
Msa0757980 AT2G23140.4 24.900 249 163 6 466 713 594 819 1.18e-12 72.0
Msa0757980 AT3G54850.1 52.000 75 36 0 274 348 249 323 1.66e-21 100
Msa0757980 AT1G71020.2 43.000 100 53 1 249 348 75 170 8.22e-21 97.1
Msa0757980 AT1G71020.1 43.000 100 53 1 249 348 223 318 1.96e-20 96.7
Msa0757980 AT3G54790.2 39.535 86 52 0 271 356 200 285 4.45e-18 89.4
Msa0757980 AT3G54790.3 39.535 86 52 0 271 356 236 321 5.54e-18 89.4
Msa0757980 AT3G54790.1 39.535 86 52 0 271 356 236 321 5.54e-18 89.4
Msa0757980 AT5G01830.1 38.710 93 48 1 274 357 275 367 4.15e-16 83.2
Msa0757980 AT1G29340.1 46.053 76 41 0 271 346 303 378 4.17e-16 83.2
Msa0757980 AT5G18340.1 31.757 148 88 2 223 358 12 158 8.57e-16 81.3
Msa0757980 AT1G49780.1 46.914 81 39 2 274 350 15 95 1.63e-15 80.1
Msa0757980 AT5G18320.1 44.444 81 44 1 271 351 70 149 4.83e-15 79.0
Msa0757980 AT3G19380.1 43.902 82 41 3 274 350 15 96 4.79e-14 75.5
Msa0757980 AT1G66160.1 39.130 92 52 2 263 350 23 114 7.27e-14 75.1
Msa0757980 AT5G37490.1 42.045 88 46 2 267 350 26 112 1.17e-13 74.3
Msa0757980 AT1G10560.1 35.849 106 67 1 248 352 262 367 4.11e-13 73.6
Msa0757980 AT2G35930.1 45.122 82 40 3 274 350 13 94 9.49e-13 71.2
Msa0757980 AT3G52450.1 43.902 82 41 3 274 350 8 89 1.43e-12 70.9
Msa0757980 AT1G01680.1 40.244 82 49 0 262 343 222 303 2.59e-12 68.9
Msa0757980 AT5G18330.1 25.000 312 183 9 271 579 63 326 4.64e-12 69.3
Msa0757980 AT3G61390.4 36.842 76 48 0 264 339 269 344 8.11e-12 68.2
Msa0757980 AT1G60190.1 39.024 82 50 0 275 356 280 361 8.62e-12 69.3
Msa0757980 AT3G61390.2 36.842 76 48 0 264 339 344 419 1.63e-11 67.8
Msa0757980 AT3G49810.1 46.970 66 35 0 274 339 65 130 2.22e-11 67.4
Msa0757980 AT1G56040.1 38.961 77 47 0 262 338 342 418 3.22e-11 66.6
Msa0757980 AT5G57035.1 43.939 66 37 0 274 339 709 774 5.88e-11 66.6

Find 165 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAATACCCTCTCCTTTC+AGG 0.129941 5_2:+13046041 Msa0757980:CDS
ATACATCTGCTGCTTAATTT+TGG 0.151569 5_2:+13049471 Msa0757980:three_prime_UTR
TGCATTGGATGCTGCAATTT+TGG 0.187482 5_2:-13048584 None:intergenic
GGATTATCTTAAGTTGATTT+TGG 0.208826 5_2:+13048948 Msa0757980:CDS
AAGTCCTTCATGACAATATT+TGG 0.222378 5_2:-13047486 None:intergenic
ATAACTTCCCAAACTCTTAA+TGG 0.232924 5_2:-13047601 None:intergenic
GCCATAATGGAAGAACTTTC+TGG 0.238374 5_2:+13048544 Msa0757980:CDS
TTGGAAGTTGCTTGCATATT+TGG 0.240940 5_2:+13045317 Msa0757980:five_prime_UTR
TTTCAAGCTCATGAGATTCT+TGG 0.241484 5_2:-13045162 None:intergenic
CCAATAGCACTTTATTAGTT+TGG 0.245701 5_2:+13045298 Msa0757980:five_prime_UTR
ACAAAGCTTAATTCCCATTC+AGG 0.250995 5_2:+13046661 Msa0757980:CDS
GTTCAATGAAATTTGTATAT+TGG 0.252298 5_2:-13045203 None:intergenic
AATCACCTCCATTAAGAGTT+TGG 0.258899 5_2:+13047593 Msa0757980:CDS
TCCAGAAAGTTCTTCCATTA+TGG 0.272030 5_2:-13048545 None:intergenic
CCAAACTAATAAAGTGCTAT+TGG 0.272229 5_2:-13045298 None:intergenic
AGATCGTTTCTCGATTAAAA+TGG 0.277574 5_2:-13047037 None:intergenic
TGAGTATGCTACATTTGTTC+TGG 0.287071 5_2:+13046879 Msa0757980:CDS
CAATAGTAACGTGTAATTCT+AGG 0.289232 5_2:+13045371 Msa0757980:five_prime_UTR
AAGAATAAACAAAATAAAAT+AGG 0.289390 5_2:-13049647 None:intergenic
GATCCTGTTATCATTGCTTC+TGG 0.293374 5_2:+13047366 Msa0757980:CDS
ACCTGAAAGGAGAGGGTATT+TGG 0.293706 5_2:-13046042 None:intergenic
TTCTTCAATAGTAGTCTAAT+TGG 0.304543 5_2:+13045343 Msa0757980:five_prime_UTR
TAAGAGCCAGTTTGGATTGA+TGG 0.313296 5_2:+13049318 Msa0757980:three_prime_UTR
TGTGAATGTGTGTGATATAT+TGG 0.316428 5_2:+13049379 Msa0757980:three_prime_UTR
TACATCAACCTTGAGGATAT+TGG 0.317123 5_2:-13047342 None:intergenic
CAACATGTCACTTGGATCAT+TGG 0.319176 5_2:+13047656 Msa0757980:CDS
CAGCATCATGAGAATATTTC+CGG 0.326397 5_2:+13046321 Msa0757980:CDS
GAACTGTTGTTGGTAATAAT+TGG 0.328521 5_2:-13049102 None:intergenic
ATGCTTGATAAGAGCCAGTT+TGG 0.336886 5_2:+13049310 Msa0757980:three_prime_UTR
ATCACCTCCATTAAGAGTTT+GGG 0.353633 5_2:+13047594 Msa0757980:CDS
CTCAACTTCATGATCTTCAA+TGG 0.371229 5_2:+13047820 Msa0757980:CDS
CACTATTGGTTCTCATTAAG+AGG 0.380327 5_2:-13045999 None:intergenic
CTTCTTGATTCAGAAGTGAT+AGG 0.387240 5_2:+13048511 Msa0757980:CDS
AATCCTAGTTTCTTCAGAAA+TGG 0.387275 5_2:-13049174 None:intergenic
ACTTATGCATCCCTTTGTTT+CGG 0.390748 5_2:-13048833 None:intergenic
AGGAGAAGTACTTGCCATAA+TGG 0.393561 5_2:+13048531 Msa0757980:CDS
TTCCGACAGAAGCTGAGTTC+CGG 0.397671 5_2:-13047994 None:intergenic
GACTGGAACCATACCCTGAA+TGG 0.398503 5_2:-13046675 None:intergenic
CTTAATTCCCATTCAGGGTA+TGG 0.404422 5_2:+13046667 Msa0757980:CDS
TCACAGAATGGAAATGATAA+AGG 0.407952 5_2:+13049003 Msa0757980:CDS
TTATTCTTATAGCATGTTGT+TGG 0.411310 5_2:-13048651 None:intergenic
AACAAAATAAAATAGGAAAC+TGG 0.414555 5_2:-13049640 None:intergenic
TAGCAGATATGATTTATATA+TGG 0.425285 5_2:+13049342 Msa0757980:three_prime_UTR
CTAGAGTTATCCCTTGCAAT+TGG 0.428602 5_2:-13047204 None:intergenic
ACTCCAGAAGCAATGATAAC+AGG 0.429341 5_2:-13047369 None:intergenic
TTTAGTAATATTCTCAAGAA+AGG 0.431108 5_2:-13049407 None:intergenic
TGCTATCCATCAATCCAAAC+TGG 0.436944 5_2:-13049324 None:intergenic
TGTTGGCAGGAAGAGGGAAA+AGG 0.439214 5_2:-13049608 None:intergenic
TGAGATGTTATATTCAGTCT+TGG 0.439810 5_2:-13047009 None:intergenic
TCCAGTCATGTTGGCTGTAG+AGG 0.442914 5_2:+13046691 Msa0757980:CDS
TGAAGATACGTTCATAAAGT+TGG 0.444353 5_2:+13048483 Msa0757980:CDS
TATGTGATGGAAGGGTTTCA+AGG 0.449063 5_2:-13045001 None:intergenic
AAATCTTCTGTATGTCTACT+TGG 0.449559 5_2:-13047552 None:intergenic
ACCTCTACAGCCAACATGAC+TGG 0.454239 5_2:-13046692 None:intergenic
GGCCGTGCTTCCTCTATGTC+CGG 0.455061 5_2:-13046340 None:intergenic
AAGAAGTTTCTAGATAAAGT+TGG 0.465531 5_2:+13047063 Msa0757980:CDS
ATTACTTCTGCAACATTGCT+CGG 0.466898 5_2:+13046423 Msa0757980:CDS
TCTGTATGTCTACTTGGATC+AGG 0.467341 5_2:-13047546 None:intergenic
TCAACTTCATGATCTTCAAT+GGG 0.469345 5_2:+13047821 Msa0757980:CDS
CATAGAGTTGATGCAAATAG+TGG 0.470306 5_2:+13046297 Msa0757980:CDS
CATCAGATTCAAGTCATGAT+TGG 0.470843 5_2:-13047715 None:intergenic
GTTGGCAGGAAGAGGGAAAA+GGG 0.474180 5_2:-13049607 None:intergenic
TATTACTTTCAGGCTGTGAC+AGG 0.476168 5_2:+13046593 Msa0757980:intron
TTGTATAGTGGTGGTGGTGG+TGG 0.476960 5_2:+13049521 Msa0757980:three_prime_UTR
ATTGGACACTTGTATTCCTC+AGG 0.479427 5_2:-13047324 None:intergenic
ACTGAATATAACATCTCAAA+AGG 0.479795 5_2:+13047014 Msa0757980:CDS
GCACGGCCAAGATGTTCATC+AGG 0.480753 5_2:+13046355 Msa0757980:CDS
CATAGCCATGAAAATTGAAA+AGG 0.488449 5_2:-13045078 None:intergenic
TCACCATTTCTGAAGAAACT+AGG 0.488630 5_2:+13049171 Msa0757980:CDS
GATTCTGCTGAAGAAGAGGC+TGG 0.489454 5_2:+13046901 Msa0757980:CDS
AAAGAATGTGGATAAGAAAG+TGG 0.491198 5_2:+13047400 Msa0757980:CDS
GACGTAAAAGCGCTGAGAGC+CGG 0.495304 5_2:+13047975 Msa0757980:CDS
AATTCTATTGCCGAAACAAA+GGG 0.496586 5_2:+13048823 Msa0757980:CDS
TACTTGATTCTGATAACAAA+GGG 0.497678 5_2:+13048626 Msa0757980:CDS
CTCTTAATGAGAACCAATAG+TGG 0.499914 5_2:+13046000 Msa0757980:exon
GGAATCTGTAGAACTGTTGT+TGG 0.501183 5_2:-13049112 None:intergenic
TCTAGTATGTTGGCAGGAAG+AGG 0.501878 5_2:-13049615 None:intergenic
ATGGAAGAACTTTCTGGAGA+TGG 0.503922 5_2:+13048550 Msa0757980:CDS
TTATGAACGTATCTTCACTT+AGG 0.504024 5_2:-13048477 None:intergenic
GAGCAATGCAAATGATCAGC+AGG 0.504932 5_2:+13047953 Msa0757980:CDS
TTTATTTATGTGTACCTGAA+AGG 0.505342 5_2:-13046055 None:intergenic
ATTGTATTGTATAGTGGTGG+TGG 0.506458 5_2:+13049515 Msa0757980:three_prime_UTR
TATCCTTGTGATACCATTGT+GGG 0.506979 5_2:+13049272 Msa0757980:three_prime_UTR
ACTGGAACCATACCCTGAAT+GGG 0.507557 5_2:-13046674 None:intergenic
GTAAGAGAATTGCTTCATCA+AGG 0.507831 5_2:+13046931 Msa0757980:CDS
CGGAATTTGTGAACAACTGC+AGG 0.508751 5_2:+13048012 Msa0757980:CDS
TGGTTAAAGTATGTGATGGA+AGG 0.511760 5_2:-13045010 None:intergenic
GGTTAAAGTATGTGATGGAA+GGG 0.511997 5_2:-13045009 None:intergenic
ATGTACATGTTCTCAAAATG+TGG 0.513586 5_2:+13048927 Msa0757980:CDS
CAAAGCTTAATTCCCATTCA+GGG 0.514357 5_2:+13046662 Msa0757980:CDS
GACTCCAAATATTGTCATGA+AGG 0.514758 5_2:+13047482 Msa0757980:CDS
TCTATTCCTGATGAACATCT+TGG 0.521143 5_2:-13046361 None:intergenic
GCATGGAAACTTAATGTTGG+TGG 0.522527 5_2:+13047143 Msa0757980:CDS
GATAAGAAAGTGGATTAATG+AGG 0.523558 5_2:+13047410 Msa0757980:CDS
ACAGTAGGCACTGTAACAGT+AGG 0.525492 5_2:+13049552 Msa0757980:three_prime_UTR
CTGGTCTCTAGTATGTTGGC+AGG 0.529952 5_2:-13049621 None:intergenic
GGAATGCAGCAAACTCTACC+TGG 0.530347 5_2:+13046444 Msa0757980:CDS
GAAGTTGCTTGCATATTTGG+TGG 0.533912 5_2:+13045320 Msa0757980:five_prime_UTR
TTTGGAGTCAATGTCATATG+AGG 0.534604 5_2:-13047468 None:intergenic
AACATTTGTGATACTGAAGA+AGG 0.537856 5_2:+13048796 Msa0757980:CDS
CATCTTAGTAGTAAAATCAA+TGG 0.539707 5_2:+13045247 Msa0757980:five_prime_UTR
TGGACAAACTTCACTGTTGT+AGG 0.541725 5_2:-13048686 None:intergenic
GACATGTTGGTAAGATCCAT+TGG 0.542509 5_2:-13047642 None:intergenic
GCATGAACTGTATGCTTGTG+AGG 0.545176 5_2:-13047765 None:intergenic
GTGGTGGTTATGTTTACAGT+AGG 0.546072 5_2:+13049537 Msa0757980:three_prime_UTR
CAATGATCTGAACTTGCCAA+TGG 0.550541 5_2:+13047626 Msa0757980:CDS
TTACTTGATTCTGATAACAA+AGG 0.553043 5_2:+13048625 Msa0757980:CDS
AGGTTCAACCTCAGATTCCA+TGG 0.554010 5_2:+13046951 Msa0757980:CDS
CATGGAAACTTAATGTTGGT+GGG 0.555579 5_2:+13047144 Msa0757980:CDS
GATTTCTTCGTCGAGAATCA+CGG 0.555802 5_2:-13047227 None:intergenic
GAGAATATTTCCGGACATAG+AGG 0.559119 5_2:+13046330 Msa0757980:CDS
TTTAACTTCAAAGTCGTCCA+TGG 0.559348 5_2:-13046968 None:intergenic
GATCTTACCAACATGTCACT+TGG 0.560709 5_2:+13047648 Msa0757980:CDS
TTCTGTGAGATATAGAAAAG+AGG 0.564373 5_2:-13048988 None:intergenic
GAAACTGGTCTCTAGTATGT+TGG 0.564897 5_2:-13049625 None:intergenic
GGGTATGGTTCCAGTCATGT+TGG 0.567915 5_2:+13046682 Msa0757980:CDS
ATGTCTACTTGGATCAGGAA+TGG 0.568043 5_2:-13047541 None:intergenic
AAATTCTATTGCCGAAACAA+AGG 0.571416 5_2:+13048822 Msa0757980:CDS
CAATGATCCAAGTGACATGT+TGG 0.572790 5_2:-13047655 None:intergenic
TCTGGATTCTGCTGAAGAAG+AGG 0.573607 5_2:+13046897 Msa0757980:CDS
TTATTGGTTAAAGTATGTGA+TGG 0.574426 5_2:-13045014 None:intergenic
ATTGTAATTGTATTGTATAG+TGG 0.575715 5_2:+13049509 Msa0757980:three_prime_UTR
TAAGACAGAAGTGAGTGCAT+TGG 0.577623 5_2:-13048599 None:intergenic
AGCCGGAACTCAGCTTCTGT+CGG 0.579942 5_2:+13047992 Msa0757980:CDS
ATTGCTGAAATACTTGAGAG+TGG 0.582850 5_2:+13048859 Msa0757980:CDS
GGACACTTGTATTCCTCAGG+TGG 0.587812 5_2:-13047321 None:intergenic
GTATTGTATAGTGGTGGTGG+TGG 0.590239 5_2:+13049518 Msa0757980:three_prime_UTR
ACACACATTCACAAAGATTG+AGG 0.593009 5_2:-13049369 None:intergenic
CTAGTATGTTGGCAGGAAGA+GGG 0.593142 5_2:-13049614 None:intergenic
CAGTAGGCACTGTAACAGTA+GGG 0.595779 5_2:+13049553 Msa0757980:three_prime_UTR
TTCCGGACATAGAGGAAGCA+CGG 0.596773 5_2:+13046338 Msa0757980:CDS
CTGACACTGACAAAGTAGCT+TGG 0.600806 5_2:-13047896 None:intergenic
ATTCTGCTGAAGAAGAGGCT+GGG 0.601067 5_2:+13046902 Msa0757980:CDS
GAGTAACATATGAAAGAATG+TGG 0.601967 5_2:+13047388 Msa0757980:CDS
ATAAGAAAGTGGATTAATGA+GGG 0.602588 5_2:+13047411 Msa0757980:CDS
TATGTGTACCTGAAAGGAGA+GGG 0.606017 5_2:-13046049 None:intergenic
CAGTGAAGTTTGTCCACACA+TGG 0.607997 5_2:+13048693 Msa0757980:CDS
AATGTTGGTGGGAGAACACA+TGG 0.609389 5_2:+13047155 Msa0757980:CDS
GAATGGAAATGATAAAGGAA+AGG 0.610121 5_2:+13049008 Msa0757980:CDS
GATGAATCAGTCGGATAGAG+TGG 0.610590 5_2:+13047296 Msa0757980:CDS
AATGTGTTGAGAATGAAGAT+AGG 0.612607 5_2:+13049070 Msa0757980:CDS
TCCTTGTGATACCATTGTGG+GGG 0.616090 5_2:+13049274 Msa0757980:three_prime_UTR
CAAAATATGAAGCAATTCCA+CGG 0.616213 5_2:-13049037 None:intergenic
TAGAGAAGAACCAATTGCAA+GGG 0.618427 5_2:+13047194 Msa0757980:CDS
CATTGAAGATCATGAAGTTG+AGG 0.619411 5_2:-13047819 None:intergenic
TAAAACTGAGATGAATCAGT+CGG 0.620886 5_2:+13047287 Msa0757980:CDS
GGTCTTAACTTGTGACACAT+CGG 0.622908 5_2:-13047694 None:intergenic
AAAGCATGGAAACTTAATGT+TGG 0.624675 5_2:+13047140 Msa0757980:CDS
GTAGAGAAGAACCAATTGCA+AGG 0.624828 5_2:+13047193 Msa0757980:CDS
AAAGTCGTCCATGGAATCTG+AGG 0.626252 5_2:-13046959 None:intergenic
TTATGTGTACCTGAAAGGAG+AGG 0.628672 5_2:-13046050 None:intergenic
ACAAGTGTCCAATATCCTCA+AGG 0.631386 5_2:+13047334 Msa0757980:CDS
ATATCCTTGTGATACCATTG+TGG 0.633299 5_2:+13049271 Msa0757980:three_prime_UTR
GGATAGAGTGGCACCACCTG+AGG 0.636060 5_2:+13047308 Msa0757980:CDS
GTAATTGTATTGTATAGTGG+TGG 0.647763 5_2:+13049512 Msa0757980:three_prime_UTR
ACTTCCCAAACTCTTAATGG+AGG 0.650645 5_2:-13047598 None:intergenic
ATCCTTGTGATACCATTGTG+GGG 0.650807 5_2:+13049273 Msa0757980:three_prime_UTR
TAAAGGAAAGGAAAGTGCCG+TGG 0.657866 5_2:+13049020 Msa0757980:CDS
CACATGCATCAAAACCCAGA+AGG 0.661406 5_2:+13049214 Msa0757980:CDS
CCTTGTGATACCATTGTGGG+GGG 0.667814 5_2:+13049275 Msa0757980:three_prime_UTR
TGAAGGACTTGATATCAAAG+TGG 0.687601 5_2:+13047499 Msa0757980:CDS
AGGATCATACATCAACCTTG+AGG 0.687795 5_2:-13047349 None:intergenic
CAATTGACAGATACCATGTG+TGG 0.700444 5_2:-13048706 None:intergenic
GTTGGTAATAATTGGCTGAG+AGG 0.708552 5_2:-13049094 None:intergenic
ATTATGACTTGAGAATGCTG+AGG 0.735899 5_2:-13049250 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATAAACAAAATAAAAT+AGG - chr5_2:13049650-13049669 None:intergenic 10.0%
!! GAACAAAAGAAATAAAAAAA+AGG - chr5_2:13045143-13045162 None:intergenic 15.0%
!!! GTTTCTATTATTTTATCTTT+AGG + chr5_2:13045419-13045438 Msa0757980:intron 15.0%
!!! TGTAATTTTTGTCAAAATTA+CGG + chr5_2:13045656-13045675 Msa0757980:intron 15.0%
!! ACAATTGAAAATAGTTTATA+TGG - chr5_2:13048108-13048127 None:intergenic 15.0%
!! TATAAATAATTAAGTTCCTA+AGG + chr5_2:13048244-13048263 Msa0757980:intron 15.0%
!!! GAAAAAATAAGAGGTATTAT+TGG - chr5_2:13045033-13045052 None:intergenic 20.0%
!!! ATTATCCTTTTCAATTTTCA+TGG + chr5_2:13045073-13045092 Msa0757980:five_prime_UTR 20.0%
!! GTTCAATGAAATTTGTATAT+TGG - chr5_2:13045206-13045225 None:intergenic 20.0%
!! ATCAACTTTGATTAATCAAA+TGG + chr5_2:13045538-13045557 Msa0757980:intron 20.0%
!!! TTGGTTTTAATATTACTTTC+AGG + chr5_2:13046583-13046602 Msa0757980:intron 20.0%
!! TATATGTCCAAAAACAAAAA+AGG + chr5_2:13047440-13047459 Msa0757980:CDS 20.0%
!!! ACTATTTTCAATTGTAAATG+TGG + chr5_2:13048113-13048132 Msa0757980:intron 20.0%
!! AAATTTATAATACCAAAAGC+AGG - chr5_2:13048412-13048431 None:intergenic 20.0%
!!! TGAAAAAAGGTAACAATTTT+GGG - chr5_2:13048735-13048754 None:intergenic 20.0%
!!! TTGAAAAAAGGTAACAATTT+TGG - chr5_2:13048736-13048755 None:intergenic 20.0%
!! TAGCAGATATGATTTATATA+TGG + chr5_2:13049342-13049361 Msa0757980:three_prime_UTR 20.0%
!! TTTAGTAATATTCTCAAGAA+AGG - chr5_2:13049410-13049429 None:intergenic 20.0%
!! ATTGTAATTGTATTGTATAG+TGG + chr5_2:13049509-13049528 Msa0757980:three_prime_UTR 20.0%
!! AACAAAATAAAATAGGAAAC+TGG - chr5_2:13049643-13049662 None:intergenic 20.0%
!!! GATATCTGTTTTTACAATTA+AGG - chr5_2:13049707-13049726 None:intergenic 20.0%
!! TTATTGGTTAAAGTATGTGA+TGG - chr5_2:13045017-13045036 None:intergenic 25.0%
! TGTAGTCATGAAAAAATAAG+AGG - chr5_2:13045042-13045061 None:intergenic 25.0%
! CATCTTAGTAGTAAAATCAA+TGG + chr5_2:13045247-13045266 Msa0757980:five_prime_UTR 25.0%
! TTCTTCAATAGTAGTCTAAT+TGG + chr5_2:13045343-13045362 Msa0757980:five_prime_UTR 25.0%
!!! AATAGAAACTTTTGAGAGTA+AGG - chr5_2:13045408-13045427 None:intergenic 25.0%
!!! ATCAAAGTTGATAAATTCAG+AGG - chr5_2:13045530-13045549 None:intergenic 25.0%
! TAGTGAGTTTGACAAAATTT+AGG + chr5_2:13045603-13045622 Msa0757980:intron 25.0%
! TTGTAGCTTCAACAAAATTA+TGG - chr5_2:13045634-13045653 None:intergenic 25.0%
!! AATTTTTGTCAAAATTACGG+TGG + chr5_2:13045659-13045678 Msa0757980:intron 25.0%
!!! AGTTTATTTTGGAATACTCT+TGG - chr5_2:13045870-13045889 None:intergenic 25.0%
! TTGTTCTTTCATTACAGTTA+AGG + chr5_2:13045909-13045928 Msa0757980:intron 25.0%
! TTTATTTATGTGTACCTGAA+AGG - chr5_2:13046058-13046077 None:intergenic 25.0%
! AACAAAATAGTGAAATGCAA+TGG - chr5_2:13046083-13046102 None:intergenic 25.0%
!! ATATCGTTTGTTAGTTAGAT+TGG - chr5_2:13046153-13046172 None:intergenic 25.0%
! CGGAATTTATGTTAATTAAC+AGG + chr5_2:13046258-13046277 Msa0757980:intron 25.0%
! GAAAAAAAAAAATACACACC+AGG - chr5_2:13046465-13046484 None:intergenic 25.0%
!!! AGTTTGGATTAACTTGTTTA+TGG - chr5_2:13046813-13046832 None:intergenic 25.0%
! ACTGAATATAACATCTCAAA+AGG + chr5_2:13047014-13047033 Msa0757980:CDS 25.0%
!! AAGAAGTTTCTAGATAAAGT+TGG + chr5_2:13047063-13047082 Msa0757980:CDS 25.0%
! ATGTATCTAATGAAAAAGCA+TGG + chr5_2:13047126-13047145 Msa0757980:CDS 25.0%
!!! ATAAGAAAGTGGATTAATGA+GGG + chr5_2:13047411-13047430 Msa0757980:CDS 25.0%
!! TACAGAAGATTTTATGTTGT+TGG + chr5_2:13047563-13047582 Msa0757980:CDS 25.0%
! ACCATACAAAGATCAAAATT+GGG - chr5_2:13048058-13048077 None:intergenic 25.0%
!! TCTACAATTTCAGAAACTTT+TGG - chr5_2:13048199-13048218 None:intergenic 25.0%
!!! AAGTTTCTGAAATTGTAGAA+AGG + chr5_2:13048200-13048219 Msa0757980:intron 25.0%
!!! TGTGTGTAATTTTTCAGAAA+TGG + chr5_2:13048445-13048464 Msa0757980:intron 25.0%
!!! GTAATTTTTCAGAAATGGTA+TGG + chr5_2:13048450-13048469 Msa0757980:intron 25.0%
! TTACTTGATTCTGATAACAA+AGG + chr5_2:13048625-13048644 Msa0757980:CDS 25.0%
! TACTTGATTCTGATAACAAA+GGG + chr5_2:13048626-13048645 Msa0757980:CDS 25.0%
! TTATTCTTATAGCATGTTGT+TGG - chr5_2:13048654-13048673 None:intergenic 25.0%
!!! GGATTATCTTAAGTTGATTT+TGG + chr5_2:13048948-13048967 Msa0757980:CDS 25.0%
!!! TTTGATGATCTATTTTCTTC+AGG - chr5_2:13049147-13049166 None:intergenic 25.0%
CATAGCCATGAAAATTGAAA+AGG - chr5_2:13045081-13045100 None:intergenic 30.0%
! GATTTTACTACTAAGATGCA+AGG - chr5_2:13045245-13045264 None:intergenic 30.0%
! CCAAACTAATAAAGTGCTAT+TGG - chr5_2:13045301-13045320 None:intergenic 30.0%
CCAATAGCACTTTATTAGTT+TGG + chr5_2:13045298-13045317 Msa0757980:five_prime_UTR 30.0%
CAATAGTAACGTGTAATTCT+AGG + chr5_2:13045371-13045390 Msa0757980:five_prime_UTR 30.0%
TTCAAAGTTCAAACTGTGTT+TGG + chr5_2:13045442-13045461 Msa0757980:intron 30.0%
TCAGAGGTTAACAATTTGAT+TGG - chr5_2:13045514-13045533 None:intergenic 30.0%
! CAAATGGCTTACGATTTTAT+TGG + chr5_2:13045554-13045573 Msa0757980:intron 30.0%
AACTCACTATGATCTAAACA+TGG - chr5_2:13045592-13045611 None:intergenic 30.0%
! ATGATAATTTGCCAGAATCA+CGG - chr5_2:13045689-13045708 None:intergenic 30.0%
! AGAGAAGGACAAGTTTATTT+TGG - chr5_2:13045881-13045900 None:intergenic 30.0%
AGAACAAAATCTACTAGAGA+AGG - chr5_2:13045896-13045915 None:intergenic 30.0%
TCGCTTAAACTCTTATAACT+TGG + chr5_2:13046210-13046229 Msa0757980:intron 30.0%
!!! AGGTTGCATAATAGTTTTGT+TGG + chr5_2:13046564-13046583 Msa0757980:intron 30.0%
!!! CTAATGACTTTTTCGCTTTT+TGG - chr5_2:13046640-13046659 None:intergenic 30.0%
AGAAAGGATAAAACTCAGTT+TGG - chr5_2:13046829-13046848 None:intergenic 30.0%
TGAGATGTTATATTCAGTCT+TGG - chr5_2:13047012-13047031 None:intergenic 30.0%
AGATCGTTTCTCGATTAAAA+TGG - chr5_2:13047040-13047059 None:intergenic 30.0%
! TCAAGATTGTCTTCTTTTTC+GGG - chr5_2:13047098-13047117 None:intergenic 30.0%
! CTCAAGATTGTCTTCTTTTT+CGG - chr5_2:13047099-13047118 None:intergenic 30.0%
AAAGCATGGAAACTTAATGT+TGG + chr5_2:13047140-13047159 Msa0757980:CDS 30.0%
TAAAACTGAGATGAATCAGT+CGG + chr5_2:13047287-13047306 Msa0757980:CDS 30.0%
GAGTAACATATGAAAGAATG+TGG + chr5_2:13047388-13047407 Msa0757980:CDS 30.0%
AAAGAATGTGGATAAGAAAG+TGG + chr5_2:13047400-13047419 Msa0757980:CDS 30.0%
!! GATAAGAAAGTGGATTAATG+AGG + chr5_2:13047410-13047429 Msa0757980:CDS 30.0%
!!! GGTAGTTCCTTTTTTGTTTT+TGG - chr5_2:13047450-13047469 None:intergenic 30.0%
AAGTCCTTCATGACAATATT+TGG - chr5_2:13047489-13047508 None:intergenic 30.0%
TCAAAGTGGTGCAAAAATAA+TGG + chr5_2:13047513-13047532 Msa0757980:CDS 30.0%
AAATCTTCTGTATGTCTACT+TGG - chr5_2:13047555-13047574 None:intergenic 30.0%
ATAACTTCCCAAACTCTTAA+TGG - chr5_2:13047604-13047623 None:intergenic 30.0%
TCAACTTCATGATCTTCAAT+GGG + chr5_2:13047821-13047840 Msa0757980:CDS 30.0%
CACCATACAAAGATCAAAAT+TGG - chr5_2:13048059-13048078 None:intergenic 30.0%
! ACTTTTGGATGATTCACTTT+AGG - chr5_2:13048184-13048203 None:intergenic 30.0%
CAAGAAACTTAAAATTTGCG+TGG - chr5_2:13048348-13048367 None:intergenic 30.0%
! AATCAAGCAAACGAATTTTC+AGG - chr5_2:13048375-13048394 None:intergenic 30.0%
TTATGAACGTATCTTCACTT+AGG - chr5_2:13048480-13048499 None:intergenic 30.0%
TGAAGATACGTTCATAAAGT+TGG + chr5_2:13048483-13048502 Msa0757980:CDS 30.0%
AACATTTGTGATACTGAAGA+AGG + chr5_2:13048796-13048815 Msa0757980:CDS 30.0%
AAATTCTATTGCCGAAACAA+AGG + chr5_2:13048822-13048841 Msa0757980:CDS 30.0%
AATTCTATTGCCGAAACAAA+GGG + chr5_2:13048823-13048842 Msa0757980:CDS 30.0%
ATGTACATGTTCTCAAAATG+TGG + chr5_2:13048927-13048946 Msa0757980:CDS 30.0%
TTCTGTGAGATATAGAAAAG+AGG - chr5_2:13048991-13049010 None:intergenic 30.0%
! CTTTTCTATATCTCACAGAA+TGG + chr5_2:13048991-13049010 Msa0757980:CDS 30.0%
TCACAGAATGGAAATGATAA+AGG + chr5_2:13049003-13049022 Msa0757980:CDS 30.0%
GAATGGAAATGATAAAGGAA+AGG + chr5_2:13049008-13049027 Msa0757980:CDS 30.0%
CAAAATATGAAGCAATTCCA+CGG - chr5_2:13049040-13049059 None:intergenic 30.0%
AATGTGTTGAGAATGAAGAT+AGG + chr5_2:13049070-13049089 Msa0757980:CDS 30.0%
!! GAACTGTTGTTGGTAATAAT+TGG - chr5_2:13049105-13049124 None:intergenic 30.0%
AATCCTAGTTTCTTCAGAAA+TGG - chr5_2:13049177-13049196 None:intergenic 30.0%
! GAAATTCATTTTCACCTTCT+GGG - chr5_2:13049231-13049250 None:intergenic 30.0%
TGTGAATGTGTGTGATATAT+TGG + chr5_2:13049379-13049398 Msa0757980:three_prime_UTR 30.0%
!! ATACATCTGCTGCTTAATTT+TGG + chr5_2:13049471-13049490 Msa0757980:three_prime_UTR 30.0%
GTAATTGTATTGTATAGTGG+TGG + chr5_2:13049512-13049531 Msa0757980:three_prime_UTR 30.0%
! GGTTAAAGTATGTGATGGAA+GGG - chr5_2:13045012-13045031 None:intergenic 35.0%
! TGGTTAAAGTATGTGATGGA+AGG - chr5_2:13045013-13045032 None:intergenic 35.0%
TTTCAAGCTCATGAGATTCT+TGG - chr5_2:13045165-13045184 None:intergenic 35.0%
GCAAGGTGAACAAAAAACAA+CGG - chr5_2:13045228-13045247 None:intergenic 35.0%
TTGGAAGTTGCTTGCATATT+TGG + chr5_2:13045317-13045336 Msa0757980:five_prime_UTR 35.0%
GGTTAACAATTTGATTGGAG+AGG - chr5_2:13045509-13045528 None:intergenic 35.0%
CTAAATTGAGAAGTGAGAAG+AGG - chr5_2:13045814-13045833 None:intergenic 35.0%
CTCTTCTCACTTCTCAATTT+AGG + chr5_2:13045812-13045831 Msa0757980:intron 35.0%
!! CACTATTGGTTCTCATTAAG+AGG - chr5_2:13046002-13046021 None:intergenic 35.0%
CTCTTAATGAGAACCAATAG+TGG + chr5_2:13046000-13046019 Msa0757980:exon 35.0%
AACCAATAGTGGTGAAAAAG+TGG + chr5_2:13046011-13046030 Msa0757980:CDS 35.0%
TAACTTGGATGTTCGAACTA+AGG + chr5_2:13046225-13046244 Msa0757980:intron 35.0%
CGAACTAAGGATTCTATTGA+CGG + chr5_2:13046238-13046257 Msa0757980:intron 35.0%
!! CATAGAGTTGATGCAAATAG+TGG + chr5_2:13046297-13046316 Msa0757980:CDS 35.0%
CAGCATCATGAGAATATTTC+CGG + chr5_2:13046321-13046340 Msa0757980:CDS 35.0%
TCTATTCCTGATGAACATCT+TGG - chr5_2:13046364-13046383 None:intergenic 35.0%
ATTACTTCTGCAACATTGCT+CGG + chr5_2:13046423-13046442 Msa0757980:CDS 35.0%
ACCACTGTTTCATAATCAAC+AGG + chr5_2:13046544-13046563 Msa0757980:intron 35.0%
! ACCTGTTGATTATGAAACAG+TGG - chr5_2:13046548-13046567 None:intergenic 35.0%
ACAAAGCTTAATTCCCATTC+AGG + chr5_2:13046661-13046680 Msa0757980:CDS 35.0%
CAAAGCTTAATTCCCATTCA+GGG + chr5_2:13046662-13046681 Msa0757980:CDS 35.0%
!! AGTTTTATCCTTTCTGTTGC+AGG + chr5_2:13046834-13046853 Msa0757980:intron 35.0%
TGAGTATGCTACATTTGTTC+TGG + chr5_2:13046879-13046898 Msa0757980:CDS 35.0%
GTAAGAGAATTGCTTCATCA+AGG + chr5_2:13046931-13046950 Msa0757980:CDS 35.0%
! TTTAACTTCAAAGTCGTCCA+TGG - chr5_2:13046971-13046990 None:intergenic 35.0%
CATGGAAACTTAATGTTGGT+GGG + chr5_2:13047144-13047163 Msa0757980:CDS 35.0%
TAGAGAAGAACCAATTGCAA+GGG + chr5_2:13047194-13047213 Msa0757980:CDS 35.0%
TACATCAACCTTGAGGATAT+TGG - chr5_2:13047345-13047364 None:intergenic 35.0%
! TTTGGAGTCAATGTCATATG+AGG - chr5_2:13047471-13047490 None:intergenic 35.0%
GACTCCAAATATTGTCATGA+AGG + chr5_2:13047482-13047501 Msa0757980:CDS 35.0%
TGAAGGACTTGATATCAAAG+TGG + chr5_2:13047499-13047518 Msa0757980:CDS 35.0%
AATCACCTCCATTAAGAGTT+TGG + chr5_2:13047593-13047612 Msa0757980:CDS 35.0%
ATCACCTCCATTAAGAGTTT+GGG + chr5_2:13047594-13047613 Msa0757980:CDS 35.0%
CATCAGATTCAAGTCATGAT+TGG - chr5_2:13047718-13047737 None:intergenic 35.0%
CATTGAAGATCATGAAGTTG+AGG - chr5_2:13047822-13047841 None:intergenic 35.0%
CTCAACTTCATGATCTTCAA+TGG + chr5_2:13047820-13047839 Msa0757980:CDS 35.0%
TTGCTCAAAAATCTGACTAC+TGG - chr5_2:13047939-13047958 None:intergenic 35.0%
CAAAGATCAAAATTGGGCAA+GGG - chr5_2:13048052-13048071 None:intergenic 35.0%
ACAAAGATCAAAATTGGGCA+AGG - chr5_2:13048053-13048072 None:intergenic 35.0%
!! GCCCAATTTTGATCTTTGTA+TGG + chr5_2:13048054-13048073 Msa0757980:intron 35.0%
ATCCTCAATCATATCCATGT+CGG - chr5_2:13048274-13048293 None:intergenic 35.0%
AAAATTTGCGTGGATCAACT+TGG - chr5_2:13048338-13048357 None:intergenic 35.0%
! TTTATCTTTGCACCTGCTTT+TGG + chr5_2:13048397-13048416 Msa0757980:intron 35.0%
CTTCTTGATTCAGAAGTGAT+AGG + chr5_2:13048511-13048530 Msa0757980:CDS 35.0%
TCCAGAAAGTTCTTCCATTA+TGG - chr5_2:13048548-13048567 None:intergenic 35.0%
! ACAGCTCTGTCTTTGAAAAA+AGG - chr5_2:13048748-13048767 None:intergenic 35.0%
! ACTTATGCATCCCTTTGTTT+CGG - chr5_2:13048836-13048855 None:intergenic 35.0%
ATTGCTGAAATACTTGAGAG+TGG + chr5_2:13048859-13048878 Msa0757980:CDS 35.0%
! ATTTTCTTCAGGATGACTCT+TGG - chr5_2:13049136-13049155 None:intergenic 35.0%
! TCACCATTTCTGAAGAAACT+AGG + chr5_2:13049171-13049190 Msa0757980:CDS 35.0%
! GGAAATTCATTTTCACCTTC+TGG - chr5_2:13049232-13049251 None:intergenic 35.0%
ATTATGACTTGAGAATGCTG+AGG - chr5_2:13049253-13049272 None:intergenic 35.0%
ATATCCTTGTGATACCATTG+TGG + chr5_2:13049271-13049290 Msa0757980:three_prime_UTR 35.0%
TATCCTTGTGATACCATTGT+GGG + chr5_2:13049272-13049291 Msa0757980:three_prime_UTR 35.0%
ACACACATTCACAAAGATTG+AGG - chr5_2:13049372-13049391 None:intergenic 35.0%
! TAATTTTGGACTGTAACAGC+AGG + chr5_2:13049485-13049504 Msa0757980:three_prime_UTR 35.0%
ATTGTATTGTATAGTGGTGG+TGG + chr5_2:13049515-13049534 Msa0757980:three_prime_UTR 35.0%
GAAGTTGCTTGCATATTTGG+TGG + chr5_2:13045320-13045339 Msa0757980:five_prime_UTR 40.0%
CTACAGCTACATATCAGACA+AGG - chr5_2:13045973-13045992 None:intergenic 40.0%
CTTGTCTGATATGTAGCTGT+AGG + chr5_2:13045971-13045990 Msa0757980:intron 40.0%
!!! CTCCACTTTTTCACCACTAT+TGG - chr5_2:13046016-13046035 None:intergenic 40.0%
TATGTGTACCTGAAAGGAGA+GGG - chr5_2:13046052-13046071 None:intergenic 40.0%
TTATGTGTACCTGAAAGGAG+AGG - chr5_2:13046053-13046072 None:intergenic 40.0%
GAGAATATTTCCGGACATAG+AGG + chr5_2:13046330-13046349 Msa0757980:CDS 40.0%
TATTACTTTCAGGCTGTGAC+AGG + chr5_2:13046593-13046612 Msa0757980:intron 40.0%
CTTAATTCCCATTCAGGGTA+TGG + chr5_2:13046667-13046686 Msa0757980:CDS 40.0%
TCTAGAAACCTGCAACAGAA+AGG - chr5_2:13046845-13046864 None:intergenic 40.0%
!!! GTCTTCTTTTTCGGGTCATT+TGG - chr5_2:13047090-13047109 None:intergenic 40.0%
GCATGGAAACTTAATGTTGG+TGG + chr5_2:13047143-13047162 Msa0757980:CDS 40.0%
GTAGAGAAGAACCAATTGCA+AGG + chr5_2:13047193-13047212 Msa0757980:CDS 40.0%
CTAGAGTTATCCCTTGCAAT+TGG - chr5_2:13047207-13047226 None:intergenic 40.0%
GATTTCTTCGTCGAGAATCA+CGG - chr5_2:13047230-13047249 None:intergenic 40.0%
ATTGGACACTTGTATTCCTC+AGG - chr5_2:13047327-13047346 None:intergenic 40.0%
ACAAGTGTCCAATATCCTCA+AGG + chr5_2:13047334-13047353 Msa0757980:CDS 40.0%
AGGATCATACATCAACCTTG+AGG - chr5_2:13047352-13047371 None:intergenic 40.0%
GATCCTGTTATCATTGCTTC+TGG + chr5_2:13047366-13047385 Msa0757980:CDS 40.0%
ACTCCAGAAGCAATGATAAC+AGG - chr5_2:13047372-13047391 None:intergenic 40.0%
ATGTCTACTTGGATCAGGAA+TGG - chr5_2:13047544-13047563 None:intergenic 40.0%
TCTGTATGTCTACTTGGATC+AGG - chr5_2:13047549-13047568 None:intergenic 40.0%
ACTTCCCAAACTCTTAATGG+AGG - chr5_2:13047601-13047620 None:intergenic 40.0%
CAATGATCTGAACTTGCCAA+TGG + chr5_2:13047626-13047645 Msa0757980:CDS 40.0%
! GACATGTTGGTAAGATCCAT+TGG - chr5_2:13047645-13047664 None:intergenic 40.0%
GATCTTACCAACATGTCACT+TGG + chr5_2:13047648-13047667 Msa0757980:CDS 40.0%
CAATGATCCAAGTGACATGT+TGG - chr5_2:13047658-13047677 None:intergenic 40.0%
! CAACATGTCACTTGGATCAT+TGG + chr5_2:13047656-13047675 Msa0757980:CDS 40.0%
!! GGTCTTAACTTGTGACACAT+CGG - chr5_2:13047697-13047716 None:intergenic 40.0%
!! AGGAGAAGTACTTGCCATAA+TGG + chr5_2:13048531-13048550 Msa0757980:CDS 40.0%
! GCCATAATGGAAGAACTTTC+TGG + chr5_2:13048544-13048563 Msa0757980:CDS 40.0%
! ATGGAAGAACTTTCTGGAGA+TGG + chr5_2:13048550-13048569 Msa0757980:CDS 40.0%
! TGCATTGGATGCTGCAATTT+TGG - chr5_2:13048587-13048606 None:intergenic 40.0%
TAAGACAGAAGTGAGTGCAT+TGG - chr5_2:13048602-13048621 None:intergenic 40.0%
TGGACAAACTTCACTGTTGT+AGG - chr5_2:13048689-13048708 None:intergenic 40.0%
CAATTGACAGATACCATGTG+TGG - chr5_2:13048709-13048728 None:intergenic 40.0%
!! GTTGGTAATAATTGGCTGAG+AGG - chr5_2:13049097-13049116 None:intergenic 40.0%
GGAATCTGTAGAACTGTTGT+TGG - chr5_2:13049115-13049134 None:intergenic 40.0%
ATCCTTGTGATACCATTGTG+GGG + chr5_2:13049273-13049292 Msa0757980:three_prime_UTR 40.0%
ATGCTTGATAAGAGCCAGTT+TGG + chr5_2:13049310-13049329 Msa0757980:three_prime_UTR 40.0%
TAAGAGCCAGTTTGGATTGA+TGG + chr5_2:13049318-13049337 Msa0757980:three_prime_UTR 40.0%
TGCTATCCATCAATCCAAAC+TGG - chr5_2:13049327-13049346 None:intergenic 40.0%
GTGGTGGTTATGTTTACAGT+AGG + chr5_2:13049537-13049556 Msa0757980:three_prime_UTR 40.0%
! GAAACTGGTCTCTAGTATGT+TGG - chr5_2:13049628-13049647 None:intergenic 40.0%
TCCAAATACCCTCTCCTTTC+AGG + chr5_2:13046041-13046060 Msa0757980:CDS 45.0%
!! ACCTGAAAGGAGAGGGTATT+TGG - chr5_2:13046045-13046064 None:intergenic 45.0%
ACTGGAACCATACCCTGAAT+GGG - chr5_2:13046677-13046696 None:intergenic 45.0%
TCTGGATTCTGCTGAAGAAG+AGG + chr5_2:13046897-13046916 Msa0757980:CDS 45.0%
ATTCTGCTGAAGAAGAGGCT+GGG + chr5_2:13046902-13046921 Msa0757980:CDS 45.0%
AGGTTCAACCTCAGATTCCA+TGG + chr5_2:13046951-13046970 Msa0757980:CDS 45.0%
AAAGTCGTCCATGGAATCTG+AGG - chr5_2:13046962-13046981 None:intergenic 45.0%
! AATGTTGGTGGGAGAACACA+TGG + chr5_2:13047155-13047174 Msa0757980:CDS 45.0%
GATGAATCAGTCGGATAGAG+TGG + chr5_2:13047296-13047315 Msa0757980:CDS 45.0%
GCATGAACTGTATGCTTGTG+AGG - chr5_2:13047768-13047787 None:intergenic 45.0%
CTGACACTGACAAAGTAGCT+TGG - chr5_2:13047899-13047918 None:intergenic 45.0%
GAGCAATGCAAATGATCAGC+AGG + chr5_2:13047953-13047972 Msa0757980:CDS 45.0%
CGGAATTTGTGAACAACTGC+AGG + chr5_2:13048012-13048031 Msa0757980:CDS 45.0%
TATCCATGTCGGACAACCTT+AGG - chr5_2:13048263-13048282 None:intergenic 45.0%
GTCCGACATGGATATGATTG+AGG + chr5_2:13048269-13048288 Msa0757980:intron 45.0%
CAGTGAAGTTTGTCCACACA+TGG + chr5_2:13048693-13048712 Msa0757980:CDS 45.0%
!! TAAAGGAAAGGAAAGTGCCG+TGG + chr5_2:13049020-13049039 Msa0757980:CDS 45.0%
!! CTTCTGGGTTTTGATGCATG+TGG - chr5_2:13049216-13049235 None:intergenic 45.0%
CACATGCATCAAAACCCAGA+AGG + chr5_2:13049214-13049233 Msa0757980:CDS 45.0%
TCCTTGTGATACCATTGTGG+GGG + chr5_2:13049274-13049293 Msa0757980:three_prime_UTR 45.0%
GATATGTATTCCCCCCACAA+TGG - chr5_2:13049288-13049307 None:intergenic 45.0%
!! GTATTGTATAGTGGTGGTGG+TGG + chr5_2:13049518-13049537 Msa0757980:three_prime_UTR 45.0%
! ACAGTAGGCACTGTAACAGT+AGG + chr5_2:13049552-13049571 Msa0757980:three_prime_UTR 45.0%
! CAGTAGGCACTGTAACAGTA+GGG + chr5_2:13049553-13049572 Msa0757980:three_prime_UTR 45.0%
CTAGTATGTTGGCAGGAAGA+GGG - chr5_2:13049617-13049636 None:intergenic 45.0%
TCTAGTATGTTGGCAGGAAG+AGG - chr5_2:13049618-13049637 None:intergenic 45.0%
TTCCGGACATAGAGGAAGCA+CGG + chr5_2:13046338-13046357 Msa0757980:CDS 50.0%
GGAATGCAGCAAACTCTACC+TGG + chr5_2:13046444-13046463 Msa0757980:CDS 50.0%
GACTGGAACCATACCCTGAA+TGG - chr5_2:13046678-13046697 None:intergenic 50.0%
GGGTATGGTTCCAGTCATGT+TGG + chr5_2:13046682-13046701 Msa0757980:CDS 50.0%
! TCCAGTCATGTTGGCTGTAG+AGG + chr5_2:13046691-13046710 Msa0757980:CDS 50.0%
ACCTCTACAGCCAACATGAC+TGG - chr5_2:13046695-13046714 None:intergenic 50.0%
GATTCTGCTGAAGAAGAGGC+TGG + chr5_2:13046901-13046920 Msa0757980:CDS 50.0%
GGACACTTGTATTCCTCAGG+TGG - chr5_2:13047324-13047343 None:intergenic 50.0%
TTCCGACAGAAGCTGAGTTC+CGG - chr5_2:13047997-13048016 None:intergenic 50.0%
GTTCCTAAGGTTGTCCGACA+TGG + chr5_2:13048257-13048276 Msa0757980:intron 50.0%
TGCGTGGATCAACTTGGTTG+CGG - chr5_2:13048332-13048351 None:intergenic 50.0%
CCTTGTGATACCATTGTGGG+GGG + chr5_2:13049275-13049294 Msa0757980:three_prime_UTR 50.0%
!! TTGTATAGTGGTGGTGGTGG+TGG + chr5_2:13049521-13049540 Msa0757980:three_prime_UTR 50.0%
GTTGGCAGGAAGAGGGAAAA+GGG - chr5_2:13049610-13049629 None:intergenic 50.0%
TGTTGGCAGGAAGAGGGAAA+AGG - chr5_2:13049611-13049630 None:intergenic 50.0%
CTGGTCTCTAGTATGTTGGC+AGG - chr5_2:13049624-13049643 None:intergenic 50.0%
ACGGTGGTTCACCGTGATTC+TGG + chr5_2:13045675-13045694 Msa0757980:intron 55.0%
GCACGGCCAAGATGTTCATC+AGG + chr5_2:13046355-13046374 Msa0757980:CDS 55.0%
!! GACGTAAAAGCGCTGAGAGC+CGG + chr5_2:13047975-13047994 Msa0757980:CDS 55.0%
AGCCGGAACTCAGCTTCTGT+CGG + chr5_2:13047992-13048011 Msa0757980:CDS 55.0%
CCCCCCACAATGGTATCACA+AGG - chr5_2:13049278-13049297 None:intergenic 55.0%
GGCCGTGCTTCCTCTATGTC+CGG - chr5_2:13046343-13046362 None:intergenic 60.0%
GGATAGAGTGGCACCACCTG+AGG + chr5_2:13047308-13047327 Msa0757980:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 13045004 13049764 13045004 ID=Msa0757980;Name=Msa0757980
chr5_2 mRNA 13045004 13049764 13045004 ID=Msa0757980-mRNA-1;Parent=Msa0757980;Name=Msa0757980-mRNA-1;_AED=0.04;_eAED=0.04;_QI=408|1|1|1|0.8|0.83|6|525|768
chr5_2 exon 13045004 13045392 13045004 ID=Msa0757980-mRNA-1:exon:5835;Parent=Msa0757980-mRNA-1
chr5_2 exon 13045987 13046062 13045987 ID=Msa0757980-mRNA-1:exon:5836;Parent=Msa0757980-mRNA-1
chr5_2 exon 13046280 13046465 13046280 ID=Msa0757980-mRNA-1:exon:5837;Parent=Msa0757980-mRNA-1
chr5_2 exon 13046605 13046712 13046605 ID=Msa0757980-mRNA-1:exon:5838;Parent=Msa0757980-mRNA-1
chr5_2 exon 13046856 13048033 13046856 ID=Msa0757980-mRNA-1:exon:5839;Parent=Msa0757980-mRNA-1
chr5_2 exon 13048462 13049764 13048462 ID=Msa0757980-mRNA-1:exon:5840;Parent=Msa0757980-mRNA-1
chr5_2 five_prime_UTR 13045004 13045392 13045004 ID=Msa0757980-mRNA-1:five_prime_utr;Parent=Msa0757980-mRNA-1
chr5_2 five_prime_UTR 13045987 13046005 13045987 ID=Msa0757980-mRNA-1:five_prime_utr;Parent=Msa0757980-mRNA-1
chr5_2 CDS 13046006 13046062 13046006 ID=Msa0757980-mRNA-1:cds;Parent=Msa0757980-mRNA-1
chr5_2 CDS 13046280 13046465 13046280 ID=Msa0757980-mRNA-1:cds;Parent=Msa0757980-mRNA-1
chr5_2 CDS 13046605 13046712 13046605 ID=Msa0757980-mRNA-1:cds;Parent=Msa0757980-mRNA-1
chr5_2 CDS 13046856 13048033 13046856 ID=Msa0757980-mRNA-1:cds;Parent=Msa0757980-mRNA-1
chr5_2 CDS 13048462 13049239 13048462 ID=Msa0757980-mRNA-1:cds;Parent=Msa0757980-mRNA-1
chr5_2 three_prime_UTR 13049240 13049764 13049240 ID=Msa0757980-mRNA-1:three_prime_utr;Parent=Msa0757980-mRNA-1
Gene Sequence

>Msa0757980

ATGAGAACCAATAGTGGTGAAAAAGTGGAGACTTTTCCAAATACCCTCTCCTTTCAGGTACATAGTAAAATATGCATAGAGTTGATGCAAATAGTGGACAGCATCATGAGAATATTTCCGGACATAGAGGAAGCACGGCCAAGATGTTCATCAGGAATAGAGTCTCTATGTTTTCTGAACAACTCAATTGAGAAAGCTAAATTACTTCTGCAACATTGCTCGGAATGCAGCAAACTCTACCTGGCTGTGACAGGAGAAACAGTACTTTCGAGATGCCAAAAAGCGAAAAAGTCATTAGAACAAAGCTTAATTCCCATTCAGGGTATGGTTCCAGTCATGTTGGCTGTAGAGGTTTCTAGAATAATTGATGATCTTGAGTATGCTACATTTGTTCTGGATTCTGCTGAAGAAGAGGCTGGGAGAGTTGTAAGAGAATTGCTTCATCAAGGTTCAACCTCAGATTCCATGGACGACTTTGAAGTTAAAGCTCTTCAGTTTGCAGCACCAAGACTGAATATAACATCTCAAAAGGCCATTTTAATCGAGAAACGATCTATCAAGAAGTTTCTAGATAAAGTTGGACCAAATGACCCGAAAAAGAAGACAATCTTGAGATATCTTATGTATCTAATGAAAAAGCATGGAAACTTAATGTTGGTGGGAGAACACATGGATAATTTTTATTCTCGTAGAGAAGAACCAATTGCAAGGGATAACTCTAGCCGTGATTCTCGACGAAGAAATCGTGTTGAGTCAGATCATAGCATGAACTATGATCAGCATAAAACTGAGATGAATCAGTCGGATAGAGTGGCACCACCTGAGGAATACAAGTGTCCAATATCCTCAAGGTTGATGTATGATCCTGTTATCATTGCTTCTGGAGTAACATATGAAAGAATGTGGATAAGAAAGTGGATTAATGAGGGTAAAACTATATGTCCAAAAACAAAAAAGGAACTACCTCATATGACATTGACTCCAAATATTGTCATGAAGGACTTGATATCAAAGTGGTGCAAAAATAATGGTGTCTCCATTCCTGATCCAAGTAGACATACAGAAGATTTTATGTTGTTGGATGCTTCAATCACCTCCATTAAGAGTTTGGGAAGTTATTTCAATGATCTGAACTTGCCAATGGATCTTACCAACATGTCACTTGGATCATTGGATAATAGTTTTAGTTCCGATGTGTCACAAGTTAAGACCAATCATGACTTGAATCTGATGATGATCAAGAGTAATGAAAATTCTCAGCCTCACAAGCATACAGTTCATGCAGAGATACATGATACAGATTTGATGCTCTTGCCTCAACTTCATGATCTTCAATGGGATTCTCAATGTAAGATCATTGAAGATTTGAAAGATCATATGAAAAGCAATAGCCAAGCTACTTTGTCAGTGTCAGCTGAAAATTTAGTTGAACCAGTAGTCAGATTTTTGAGCAATGCAAATGATCAGCAGGACGTAAAAGCGCTGAGAGCCGGAACTCAGCTTCTGTCGGAATTTGTGAACAACTGCAGAAATGGTATGGCTAACCTAAGTGAAGATACGTTCATAAAGTTGGCAAATCTTCTTGATTCAGAAGTGATAGGAGAAGTACTTGCCATAATGGAAGAACTTTCTGGAGATGGAAATAGTAAAGCCAAAATTGCAGCATCCAATGCACTCACTTCTGTCTTAAAGTTACTTGATTCTGATAACAAAGGGTTCCAACAACATGCTATAAGAATAATCTACAATTTGTCCTACAACAGTGAAGTTTGTCCACACATGGTATCTGTCAATTGCATCCCAAAATTGTTACCTTTTTTCAAAGACAGAGCTGTTTTGAGATACTGCATATATATATTGAAAAACATTTGTGATACTGAAGAAGGTAGAAATTCTATTGCCGAAACAAAGGGATGCATAAGTTCTATTGCTGAAATACTTGAGAGTGGAAGTAATGAAGAGCAAGAACATGCACTTGATGTTCTACTATCTTTATGTACATGTTCTCAAAATGTGGATTATCTTAAGTTGATTTTGGATGAAGATATTATCACTCCTCTTTTCTATATCTCACAGAATGGAAATGATAAAGGAAAGGAAAGTGCCGTGGAATTGCTTCATATTTTGAGAGATGCAAAATGTGTTGAGAATGAAGATAGGTCCTCTCAGCCAATTATTACCAACAACAGTTCTACAGATTCCAAGAGTCATCCTGAAGAAAATAGATCATCAAAGAAATCACCATTTCTGAAGAAACTAGGATTGTTCTCGAAATCTACTCCACATGCATCAAAACCCAGAAGGTGA
Protein sequence

>Msa0757980

MRTNSGEKVETFPNTLSFQVHSKICIELMQIVDSIMRIFPDIEEARPRCSSGIESLCFLNNSIEKAKLLLQHCSECSKLYLAVTGETVLSRCQKAKKSLEQSLIPIQGMVPVMLAVEVSRIIDDLEYATFVLDSAEEEAGRVVRELLHQGSTSDSMDDFEVKALQFAAPRLNITSQKAILIEKRSIKKFLDKVGPNDPKKKTILRYLMYLMKKHGNLMLVGEHMDNFYSRREEPIARDNSSRDSRRRNRVESDHSMNYDQHKTEMNQSDRVAPPEEYKCPISSRLMYDPVIIASGVTYERMWIRKWINEGKTICPKTKKELPHMTLTPNIVMKDLISKWCKNNGVSIPDPSRHTEDFMLLDASITSIKSLGSYFNDLNLPMDLTNMSLGSLDNSFSSDVSQVKTNHDLNLMMIKSNENSQPHKHTVHAEIHDTDLMLLPQLHDLQWDSQCKIIEDLKDHMKSNSQATLSVSAENLVEPVVRFLSNANDQQDVKALRAGTQLLSEFVNNCRNGMANLSEDTFIKLANLLDSEVIGEVLAIMEELSGDGNSKAKIAASNALTSVLKLLDSDNKGFQQHAIRIIYNLSYNSEVCPHMVSVNCIPKLLPFFKDRAVLRYCIYILKNICDTEEGRNSIAETKGCISSIAEILESGSNEEQEHALDVLLSLCTCSQNVDYLKLILDEDIITPLFYISQNGNDKGKESAVELLHILRDAKCVENEDRSSQPIITNNSSTDSKSHPEENRSSKKSPFLKKLGLFSKSTPHASKPRR*