AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0660870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660870 MtrunA17_Chr5g0404311 95.312 768 35 1 1 768 1 767 0.0 1473
Msa0660870 MtrunA17_Chr8g0373031 56.522 759 316 6 1 756 1 748 0.0 844
Msa0660870 MtrunA17_Chr8g0377151 31.198 718 447 13 19 713 19 712 8.89e-102 330
Msa0660870 MtrunA17_Chr3g0122721 30.769 767 483 16 10 754 9 749 1.44e-99 325
Msa0660870 MtrunA17_Chr1g0155261 41.627 209 121 1 19 226 16 224 1.37e-38 143
Msa0660870 MtrunA17_Chr1g0183781 28.257 499 281 17 271 713 239 716 2.93e-30 127
Msa0660870 MtrunA17_Chr7g0237931 26.196 439 250 14 274 709 238 605 1.32e-27 118
Msa0660870 MtrunA17_Chr7g0275081 37.857 140 85 1 225 362 28 167 4.78e-27 115
Msa0660870 MtrunA17_Chr4g0044411 49.412 85 43 0 264 348 264 348 9.12e-24 107
Msa0660870 MtrunA17_Chr7g0234671 24.176 455 249 14 242 662 54 446 8.30e-23 102
Msa0660870 MtrunA17_Chr2g0284461 21.510 437 288 10 274 710 293 674 3.49e-22 102
Msa0660870 MtrunA17_Chr1g0199321 53.333 75 35 0 274 348 248 322 3.53e-22 101
Msa0660870 MtrunA17_Chr4g0031431 24.847 326 207 9 29 348 37 330 1.11e-21 100
Msa0660870 MtrunA17_Chr3g0106931 48.889 90 45 1 266 354 225 314 2.32e-21 99.8
Msa0660870 MtrunA17_Chr1g0189811 45.455 77 42 0 274 350 64 140 1.42e-20 95.5
Msa0660870 MtrunA17_Chr7g0248251 37.903 124 64 2 253 367 258 377 4.14e-20 95.5
Msa0660870 MtrunA17_Chr4g0013091 24.070 457 270 15 266 710 306 697 1.83e-19 93.6
Msa0660870 MtrunA17_Chr7g0261371 47.436 78 41 0 271 348 239 316 2.02e-19 93.6
Msa0660870 MtrunA17_Chr7g0261371 29.655 290 162 12 451 713 516 790 5.87e-12 69.3
Msa0660870 MtrunA17_Chr5g0437411 48.649 74 38 0 274 347 294 367 1.89e-18 90.1
Msa0660870 MtrunA17_Chr5g0413681 43.902 82 46 0 275 356 282 363 2.22e-17 86.7
Msa0660870 MtrunA17_Chr4g0059201 43.243 74 42 0 274 347 320 393 2.82e-16 83.2
Msa0660870 MtrunA17_Chr1g0198984 50.794 63 31 0 286 348 1 63 3.65e-16 75.1
Msa0660870 MtrunA17_Chr3g0117851 42.500 80 46 0 274 353 276 355 1.21e-15 81.3
Msa0660870 MtrunA17_Chr4g0044631 40.000 100 55 3 274 369 15 113 1.43e-15 79.7
Msa0660870 MtrunA17_Chr1g0177261 41.509 106 48 6 265 359 1 103 5.10e-14 75.1
Msa0660870 MtrunA17_Chr3g0110121 38.372 86 49 2 269 350 32 117 5.38e-14 75.1
Msa0660870 MtrunA17_Chr6g0449461 28.866 291 186 10 446 723 10 292 7.47e-14 73.6
Msa0660870 MtrunA17_Chr5g0420471 38.095 84 48 2 271 350 23 106 9.19e-14 74.3
Msa0660870 MtrunA17_Chr5g0434171 39.286 84 47 2 271 350 33 116 1.58e-13 73.6
Msa0660870 MtrunA17_Chr6g0479261 38.095 84 48 2 271 350 23 106 1.72e-13 73.6
Msa0660870 MtrunA17_Chr1g0177281 41.509 106 48 6 265 359 1 103 2.01e-13 73.2
Msa0660870 MtrunA17_Chr1g0177271 45.349 86 37 4 265 343 1 83 4.11e-13 72.4
Msa0660870 MtrunA17_Chr4g0048811 47.761 67 34 1 274 339 6 72 7.28e-13 70.5
Msa0660870 MtrunA17_Chr8g0390311 34.615 104 66 2 246 348 369 471 1.16e-12 72.0
Msa0660870 MtrunA17_Chr5g0429231 25.532 282 195 6 440 712 40 315 1.39e-12 70.1
Msa0660870 MtrunA17_Chr2g0290151 28.743 167 112 5 550 713 290 452 5.17e-12 68.9
Msa0660870 MtrunA17_Chr1g0177301 42.045 88 45 4 269 350 2 89 7.16e-12 68.2
Msa0660870 MtrunA17_Chr5g0435041 24.528 212 114 3 546 712 89 299 1.10e-11 67.4
Msa0660870 MtrunA17_Chr7g0267501 41.573 89 45 4 269 350 4 92 2.77e-11 66.2
Msa0660870 MtrunA17_Chr3g0129811 39.130 69 42 0 271 339 81 149 3.80e-11 66.2
Msa0660870 MtrunA17_Chr2g0280671 44.615 65 36 0 275 339 17 81 7.02e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660870 AT4G36550.1 36.856 757 399 17 17 756 12 706 8.85e-139 426
Msa0660870 AT1G24330.1 33.102 719 430 15 19 713 17 708 4.62e-113 361
Msa0660870 AT1G24330.2 33.102 719 430 15 19 713 17 708 4.62e-113 361
Msa0660870 AT1G67530.2 32.664 747 460 16 19 749 17 736 3.50e-110 353
Msa0660870 AT1G67530.1 32.664 747 460 16 19 749 17 736 3.50e-110 353
Msa0660870 AT1G67530.4 32.664 747 460 16 19 749 17 736 3.50e-110 353
Msa0660870 AT1G27910.1 30.590 729 433 15 19 713 17 706 4.98e-91 302
Msa0660870 AT1G67530.3 34.990 503 300 10 19 510 17 503 1.62e-85 281
Msa0660870 AT5G67340.1 25.427 468 307 12 274 710 241 697 3.27e-34 140
Msa0660870 AT5G67340.2 25.427 468 307 12 274 710 241 697 3.27e-34 140
Msa0660870 AT3G46510.1 26.807 332 210 7 23 350 31 333 2.24e-25 112
Msa0660870 AT3G07360.1 53.333 75 35 0 271 345 72 146 6.53e-25 109
Msa0660870 AT5G42340.1 26.061 330 197 9 27 349 77 366 5.71e-24 108
Msa0660870 AT2G28830.1 48.276 87 45 0 262 348 173 259 1.74e-22 102
Msa0660870 AT2G23140.1 46.591 88 47 0 267 354 227 314 4.45e-22 102
Msa0660870 AT2G23140.1 24.900 249 163 6 466 713 597 822 1.23e-12 72.0
Msa0660870 AT2G23140.2 46.591 88 47 0 267 354 224 311 4.71e-22 102
Msa0660870 AT2G23140.2 24.900 249 163 6 466 713 594 819 1.23e-12 72.0
Msa0660870 AT2G23140.4 46.591 88 47 0 267 354 224 311 4.71e-22 102
Msa0660870 AT2G23140.4 24.900 249 163 6 466 713 594 819 1.23e-12 72.0
Msa0660870 AT2G23140.3 46.591 88 47 0 267 354 35 122 4.90e-22 101
Msa0660870 AT2G23140.3 24.900 249 163 6 466 713 405 630 8.30e-13 72.4
Msa0660870 AT2G23140.5 46.591 88 47 0 267 354 35 122 4.90e-22 101
Msa0660870 AT2G23140.5 24.900 249 163 6 466 713 405 630 8.30e-13 72.4
Msa0660870 AT1G23030.1 25.291 344 234 8 259 584 227 565 5.20e-22 101
Msa0660870 AT3G54850.1 52.000 75 36 0 274 348 249 323 1.66e-21 100
Msa0660870 AT1G71020.2 43.000 100 53 1 249 348 75 170 7.58e-21 97.1
Msa0660870 AT1G71020.1 43.000 100 53 1 249 348 223 318 1.96e-20 96.7
Msa0660870 AT3G54790.2 38.372 86 53 0 271 356 200 285 1.34e-17 87.8
Msa0660870 AT3G54790.3 38.372 86 53 0 271 356 236 321 1.78e-17 87.8
Msa0660870 AT3G54790.1 38.372 86 53 0 271 356 236 321 1.78e-17 87.8
Msa0660870 AT1G29340.1 46.053 76 41 0 271 346 303 378 4.32e-16 83.2
Msa0660870 AT5G01830.1 44.444 72 40 0 274 345 275 346 6.67e-16 82.4
Msa0660870 AT5G18340.1 31.757 148 88 2 223 358 12 158 9.88e-16 80.9
Msa0660870 AT1G49780.1 46.914 81 39 2 274 350 15 95 1.58e-15 80.1
Msa0660870 AT5G18320.1 46.575 73 38 1 271 343 70 141 8.28e-15 78.2
Msa0660870 AT3G19380.1 43.902 82 41 3 274 350 15 96 4.88e-14 75.5
Msa0660870 AT1G66160.1 39.130 92 52 2 263 350 23 114 7.27e-14 75.1
Msa0660870 AT5G37490.1 42.045 88 46 2 267 350 26 112 1.14e-13 74.3
Msa0660870 AT2G35930.1 45.122 82 40 3 274 350 13 94 9.58e-13 71.2
Msa0660870 AT3G52450.1 43.902 82 41 3 274 350 8 89 1.37e-12 70.9
Msa0660870 AT1G60190.1 40.244 82 49 0 275 356 280 361 1.57e-12 71.6
Msa0660870 AT1G10560.1 34.906 106 68 1 248 352 262 367 1.69e-12 71.6
Msa0660870 AT1G01680.1 40.244 82 49 0 262 343 222 303 2.61e-12 68.9
Msa0660870 AT5G18330.1 38.571 70 42 1 271 340 63 131 7.60e-12 68.9
Msa0660870 AT3G61390.4 36.842 76 48 0 264 339 269 344 7.96e-12 68.2
Msa0660870 AT3G61390.2 36.842 76 48 0 264 339 344 419 1.60e-11 67.8
Msa0660870 AT3G49810.1 46.970 66 35 0 274 339 65 130 2.28e-11 67.4
Msa0660870 AT1G56040.1 38.961 77 47 0 262 338 342 418 3.00e-11 66.6
Msa0660870 AT5G57035.1 43.939 66 37 0 274 339 709 774 5.93e-11 66.6

Find 164 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAATACCCTCTCCTTTC+AGG 0.129941 4_3:+71994555 Msa0660870:CDS
ATACATCTGCTGCTTAATTT+TGG 0.151569 4_3:+71997999 Msa0660870:three_prime_UTR
TGCATTGGATGCTGCAATTT+TGG 0.187482 4_3:-71997112 None:intergenic
GGATTATCTTAAGTTGATTT+TGG 0.208826 4_3:+71997476 Msa0660870:CDS
AAGTCCTTCATGACAATATT+TGG 0.222378 4_3:-71996014 None:intergenic
ATAACTTCCCAAACTCTTAA+TGG 0.232924 4_3:-71996129 None:intergenic
GCCATAATGGAAGAACTTTC+TGG 0.238374 4_3:+71997072 Msa0660870:CDS
TTGGAAGTTGCTTGCATATT+TGG 0.240940 4_3:+71993802 Msa0660870:five_prime_UTR
TTTCAAGCTCATGAGATTCT+TGG 0.241484 4_3:-71993648 None:intergenic
CCAATAGCACTTTATTAGTT+TGG 0.245701 4_3:+71993783 Msa0660870:five_prime_UTR
ACAAAGCTTAATTCCCATTC+AGG 0.250995 4_3:+71995190 Msa0660870:CDS
GTTCAATGAAATTTGTATAT+TGG 0.252298 4_3:-71993688 None:intergenic
AATCACCTCCATTAAGAGTT+TGG 0.258899 4_3:+71996121 Msa0660870:CDS
TCCAGAAAGTTCTTCCATTA+TGG 0.272030 4_3:-71997073 None:intergenic
CCAAACTAATAAAGTGCTAT+TGG 0.272229 4_3:-71993783 None:intergenic
AGATCGTTTCTCGATTAAAA+TGG 0.277574 4_3:-71995565 None:intergenic
TGAGTATGCTACATTTGTTC+TGG 0.287071 4_3:+71995407 Msa0660870:CDS
CAATAGTAACGTGTAATTCT+AGG 0.289232 4_3:+71993856 Msa0660870:five_prime_UTR
AAGAATAAACAAAATAAAAT+AGG 0.289390 4_3:-71998175 None:intergenic
GATCCTGTTATCATTGCTTC+TGG 0.293374 4_3:+71995894 Msa0660870:CDS
ACCTGAAAGGAGAGGGTATT+TGG 0.293706 4_3:-71994556 None:intergenic
TTCTTCAATAGTAGTCTAAT+TGG 0.304543 4_3:+71993828 Msa0660870:five_prime_UTR
TAAGAGCCAGTTTGGATTGA+TGG 0.313296 4_3:+71997846 Msa0660870:three_prime_UTR
TGTGAATGTGTGTGATATAT+TGG 0.316428 4_3:+71997907 Msa0660870:three_prime_UTR
TACATCAACCTTGAGGATAT+TGG 0.317123 4_3:-71995870 None:intergenic
CAACATGTCACTTGGATCAT+TGG 0.319176 4_3:+71996184 Msa0660870:CDS
GAACTGTTGTTGGTAATAAT+TGG 0.328521 4_3:-71997630 None:intergenic
ATGCTTGATAAGAGCCAGTT+TGG 0.336886 4_3:+71997838 Msa0660870:three_prime_UTR
ATCACCTCCATTAAGAGTTT+GGG 0.353633 4_3:+71996122 Msa0660870:CDS
CTCAACTTCATGATCTTCAA+TGG 0.371229 4_3:+71996348 Msa0660870:CDS
CACTATTGGTTCTCATTAAG+AGG 0.380327 4_3:-71994513 None:intergenic
CTTCTTGATTCAGAAGTGAT+AGG 0.387240 4_3:+71997039 Msa0660870:CDS
AATCCTAGTTTCTTCAGAAA+TGG 0.387275 4_3:-71997702 None:intergenic
ACTTATGCATCCCTTTGTTT+CGG 0.390748 4_3:-71997361 None:intergenic
AGGAGAAGTACTTGCCATAA+TGG 0.393561 4_3:+71997059 Msa0660870:CDS
TTCCGACAGAAGCTGAGTTC+CGG 0.397671 4_3:-71996522 None:intergenic
GACTGGAACCATACCCTGAA+TGG 0.398503 4_3:-71995204 None:intergenic
CTTAATTCCCATTCAGGGTA+TGG 0.404422 4_3:+71995196 Msa0660870:CDS
TCACAGAATGGAAATGATAA+AGG 0.407952 4_3:+71997531 Msa0660870:CDS
TTATTCTTATAGCATGTTGT+TGG 0.411310 4_3:-71997179 None:intergenic
AACAAAATAAAATAGGAAAC+TGG 0.414555 4_3:-71998168 None:intergenic
TAGCAGATATGATTTATATA+TGG 0.425285 4_3:+71997870 Msa0660870:three_prime_UTR
CTAGAGTTATCCCTTGCAAT+TGG 0.428602 4_3:-71995732 None:intergenic
ACTCCAGAAGCAATGATAAC+AGG 0.429341 4_3:-71995897 None:intergenic
TTTAGTAATATTCTCAAGAA+AGG 0.431108 4_3:-71997935 None:intergenic
TGCTATCCATCAATCCAAAC+TGG 0.436944 4_3:-71997852 None:intergenic
TGTTGGCAGGAAGAGGGAAA+AGG 0.439214 4_3:-71998136 None:intergenic
TGAGATGTTATATTCAGTCT+TGG 0.439810 4_3:-71995537 None:intergenic
TCCAGTCATGTTGGCTGTAG+AGG 0.442914 4_3:+71995220 Msa0660870:CDS
TGAAGATACGTTCATAAAGT+TGG 0.444353 4_3:+71997011 Msa0660870:CDS
TATGTGATGGAAGGGTTTCA+AGG 0.449063 4_3:-71993488 None:intergenic
CATAGAATTGATGAAAATAG+TGG 0.450941 4_3:+71994811 Msa0660870:CDS
ACCTCTACAGCCAACATGAC+TGG 0.454239 4_3:-71995221 None:intergenic
AAGAAGTTTCTAGATAAAGT+TGG 0.465531 4_3:+71995591 Msa0660870:CDS
GGACGTGCTTCCTCTATGTC+TGG 0.467090 4_3:-71994854 None:intergenic
TCAACTTCATGATCTTCAAT+GGG 0.469345 4_3:+71996349 Msa0660870:CDS
TCTGTATGTCTACCTGGATC+AGG 0.469637 4_3:-71996074 None:intergenic
CATCAGATTCAAGTCATGAT+TGG 0.470843 4_3:-71996243 None:intergenic
ATTACTTCTTCAACATTGCT+CGG 0.472225 4_3:+71994937 Msa0660870:CDS
GTTGGCAGGAAGAGGGAAAA+GGG 0.474180 4_3:-71998135 None:intergenic
TATTACTTTCAGGCTGTGAC+AGG 0.476168 4_3:+71995122 Msa0660870:intron
TTGTATAGTGGTGGTGGTGG+TGG 0.476960 4_3:+71998049 Msa0660870:three_prime_UTR
ATTGGACACTTGTATTCCTC+AGG 0.479427 4_3:-71995852 None:intergenic
ACTGAATATAACATCTCAAA+AGG 0.479795 4_3:+71995542 Msa0660870:CDS
GCACGTCCAAGATGTTCATC+AGG 0.480753 4_3:+71994869 Msa0660870:CDS
TCTATTCCTGATGAACATCT+TGG 0.488308 4_3:-71994875 None:intergenic
TCACCATTTCTGAAGAAACT+AGG 0.488630 4_3:+71997699 Msa0660870:CDS
GATTCTGCTGAAGAAGAGGC+TGG 0.489454 4_3:+71995429 Msa0660870:CDS
CTTCTTAGTAGTAAAATCAA+TGG 0.490228 4_3:+71993732 Msa0660870:five_prime_UTR
AAAGAATGTGGATAAGAAAG+TGG 0.491198 4_3:+71995928 Msa0660870:CDS
GACGTAAAAGCGCTGAGAGC+CGG 0.495304 4_3:+71996503 Msa0660870:CDS
AATTCTATTGCCGAAACAAA+GGG 0.496586 4_3:+71997351 Msa0660870:CDS
TACTTGATTCTGATAACAAA+GGG 0.497678 4_3:+71997154 Msa0660870:CDS
CTCTTAATGAGAACCAATAG+TGG 0.499914 4_3:+71994514 Msa0660870:exon
GGAATCTGTAGAACTGTTGT+TGG 0.501183 4_3:-71997640 None:intergenic
TCTAGTATGTTGGCAGGAAG+AGG 0.501878 4_3:-71998143 None:intergenic
ATGGAAGAACTTTCTGGAGA+TGG 0.503922 4_3:+71997078 Msa0660870:CDS
TTATGAACGTATCTTCACTT+AGG 0.504024 4_3:-71997005 None:intergenic
GAGCAATGCAAATGATCAGC+AGG 0.504932 4_3:+71996481 Msa0660870:CDS
TTTATTTATGTGTACCTGAA+AGG 0.505342 4_3:-71994569 None:intergenic
ATTGTATTGTATAGTGGTGG+TGG 0.506458 4_3:+71998043 Msa0660870:three_prime_UTR
TATCCTTGTGATACCATTGT+GGG 0.506979 4_3:+71997800 Msa0660870:three_prime_UTR
GGTGTCTCCATTCCTGATCC+AGG 0.507047 4_3:+71996062 Msa0660870:CDS
ACTGGAACCATACCCTGAAT+GGG 0.507557 4_3:-71995203 None:intergenic
GTAAGAGAATTGCTTCATCA+AGG 0.507831 4_3:+71995459 Msa0660870:CDS
CGGAATTTGTGAACAACTGC+AGG 0.508751 4_3:+71996540 Msa0660870:CDS
TGGTTAAAGTATGTGATGGA+AGG 0.511760 4_3:-71993497 None:intergenic
GCATGCCATGAAAATTGAAA+AGG 0.511873 4_3:-71993565 None:intergenic
GGTTAAAGTATGTGATGGAA+GGG 0.511997 4_3:-71993496 None:intergenic
ATGTACATGTTCTCAAAATG+TGG 0.513586 4_3:+71997455 Msa0660870:CDS
CAAAGCTTAATTCCCATTCA+GGG 0.514357 4_3:+71995191 Msa0660870:CDS
GACTCCAAATATTGTCATGA+AGG 0.514758 4_3:+71996010 Msa0660870:CDS
GCATGGAAACTTAATGTTGG+TGG 0.522527 4_3:+71995671 Msa0660870:CDS
GATAAGAAAGTGGATTAATG+AGG 0.523558 4_3:+71995938 Msa0660870:CDS
ACAGTAGGCACTGTAACAGT+AGG 0.525492 4_3:+71998080 Msa0660870:three_prime_UTR
GAGAATATTTCCAGACATAG+AGG 0.529000 4_3:+71994844 Msa0660870:CDS
CTGGTCTCTAGTATGTTGGC+AGG 0.529952 4_3:-71998149 None:intergenic
GAAGTTGCTTGCATATTTGG+TGG 0.533912 4_3:+71993805 Msa0660870:five_prime_UTR
TTTGGAGTCAATGTCATATG+AGG 0.534604 4_3:-71995996 None:intergenic
AAATCTTCTGTATGTCTACC+TGG 0.534943 4_3:-71996080 None:intergenic
AACATTTGTGATACTGAAGA+AGG 0.537856 4_3:+71997324 Msa0660870:CDS
TGGACAAACTTCACTGTTGT+AGG 0.541725 4_3:-71997214 None:intergenic
GACATGTTGGTAAGATCCAT+TGG 0.542509 4_3:-71996170 None:intergenic
GCATGAACTGTATGCTTGTG+AGG 0.545176 4_3:-71996293 None:intergenic
GTGGTGGTTATGTTTACAGT+AGG 0.546072 4_3:+71998065 Msa0660870:three_prime_UTR
GGAATGCAGCAAACTTTACC+TGG 0.549952 4_3:+71994958 Msa0660870:CDS
CAATGATCTGAACTTGCCAA+TGG 0.550541 4_3:+71996154 Msa0660870:CDS
TTACTTGATTCTGATAACAA+AGG 0.553043 4_3:+71997153 Msa0660870:CDS
AGGTTCAACCTCAGATTCCA+TGG 0.554010 4_3:+71995479 Msa0660870:CDS
CATGGAAACTTAATGTTGGT+GGG 0.555579 4_3:+71995672 Msa0660870:CDS
GATTTCTTCGTCGAGAATCA+CGG 0.555802 4_3:-71995755 None:intergenic
TTTAACTTCAAAGTCGTCCA+TGG 0.559348 4_3:-71995496 None:intergenic
GATCTTACCAACATGTCACT+TGG 0.560709 4_3:+71996176 Msa0660870:CDS
TTCTGTGAGATATAGAAAAG+AGG 0.564373 4_3:-71997516 None:intergenic
GAAACTGGTCTCTAGTATGT+TGG 0.564897 4_3:-71998153 None:intergenic
GGGTATGGTTCCAGTCATGT+TGG 0.567915 4_3:+71995211 Msa0660870:CDS
AAATTCTATTGCCGAAACAA+AGG 0.571416 4_3:+71997350 Msa0660870:CDS
CAATGATCCAAGTGACATGT+TGG 0.572790 4_3:-71996183 None:intergenic
TCTGGATTCTGCTGAAGAAG+AGG 0.573607 4_3:+71995425 Msa0660870:CDS
TTATTGGTTAAAGTATGTGA+TGG 0.574426 4_3:-71993501 None:intergenic
ATTGTAATTGTATTGTATAG+TGG 0.575715 4_3:+71998037 Msa0660870:three_prime_UTR
TAAGACAGAAGTGAGTGCAT+TGG 0.577623 4_3:-71997127 None:intergenic
ATGTCTACCTGGATCAGGAA+TGG 0.578674 4_3:-71996069 None:intergenic
AGCCGGAACTCAGCTTCTGT+CGG 0.579942 4_3:+71996520 Msa0660870:CDS
ATTGCTGAAATACTTGAGAG+TGG 0.582850 4_3:+71997387 Msa0660870:CDS
GGACACTTGTATTCCTCAGG+TGG 0.587812 4_3:-71995849 None:intergenic
GTATTGTATAGTGGTGGTGG+TGG 0.590239 4_3:+71998046 Msa0660870:three_prime_UTR
ACACACATTCACAAAGATTG+AGG 0.593009 4_3:-71997897 None:intergenic
CTAGTATGTTGGCAGGAAGA+GGG 0.593142 4_3:-71998142 None:intergenic
CAGTAGGCACTGTAACAGTA+GGG 0.595779 4_3:+71998081 Msa0660870:three_prime_UTR
CTGACACTGACAAAGTAGCT+TGG 0.600806 4_3:-71996424 None:intergenic
ATTCTGCTGAAGAAGAGGCT+GGG 0.601067 4_3:+71995430 Msa0660870:CDS
GAGTAACATATGAAAGAATG+TGG 0.601967 4_3:+71995916 Msa0660870:CDS
ATAAGAAAGTGGATTAATGA+GGG 0.602588 4_3:+71995939 Msa0660870:CDS
TATGTGTACCTGAAAGGAGA+GGG 0.606017 4_3:-71994563 None:intergenic
CAGTGAAGTTTGTCCACACA+TGG 0.607997 4_3:+71997221 Msa0660870:CDS
AATGTTGGTGGGAGAACACA+TGG 0.609389 4_3:+71995683 Msa0660870:CDS
GAATGGAAATGATAAAGGAA+AGG 0.610121 4_3:+71997536 Msa0660870:CDS
GATGAATCAGTCGGATAGAG+TGG 0.610590 4_3:+71995824 Msa0660870:CDS
AATGTGTTGAGAATGAAGAT+AGG 0.612607 4_3:+71997598 Msa0660870:CDS
TCCTTGTGATACCATTGTGG+GGG 0.616090 4_3:+71997802 Msa0660870:three_prime_UTR
CAAAATATGAAGCAATTCCA+CGG 0.616213 4_3:-71997565 None:intergenic
TAGAGAAGAACCAATTGCAA+GGG 0.618427 4_3:+71995722 Msa0660870:CDS
CATTGAAGATCATGAAGTTG+AGG 0.619411 4_3:-71996347 None:intergenic
TAAAACTGAGATGAATCAGT+CGG 0.620886 4_3:+71995815 Msa0660870:CDS
GGTCTTAACTTGTGACACAT+CGG 0.622908 4_3:-71996222 None:intergenic
AAAGCATGGAAACTTAATGT+TGG 0.624675 4_3:+71995668 Msa0660870:CDS
GTAGAGAAGAACCAATTGCA+AGG 0.624828 4_3:+71995721 Msa0660870:CDS
AAAGTCGTCCATGGAATCTG+AGG 0.626252 4_3:-71995487 None:intergenic
TTATGTGTACCTGAAAGGAG+AGG 0.628672 4_3:-71994564 None:intergenic
ACAAGTGTCCAATATCCTCA+AGG 0.631386 4_3:+71995862 Msa0660870:CDS
ATATCCTTGTGATACCATTG+TGG 0.633299 4_3:+71997799 Msa0660870:three_prime_UTR
GGATAGAGTGGCACCACCTG+AGG 0.636060 4_3:+71995836 Msa0660870:CDS
GTAATTGTATTGTATAGTGG+TGG 0.647763 4_3:+71998040 Msa0660870:three_prime_UTR
ACTTCCCAAACTCTTAATGG+AGG 0.650645 4_3:-71996126 None:intergenic
ATCCTTGTGATACCATTGTG+GGG 0.650807 4_3:+71997801 Msa0660870:three_prime_UTR
TAAAGGAAAGGAAAGTGCCG+TGG 0.657866 4_3:+71997548 Msa0660870:CDS
CACATGCATCAAAACCCAGA+AGG 0.661406 4_3:+71997742 Msa0660870:CDS
CCTTGTGATACCATTGTGGG+GGG 0.667814 4_3:+71997803 Msa0660870:three_prime_UTR
TGAAGGACTTGATATCAAAG+TGG 0.687601 4_3:+71996027 Msa0660870:CDS
AGGATCATACATCAACCTTG+AGG 0.687795 4_3:-71995877 None:intergenic
CAATTGACAGATACCATGTG+TGG 0.700444 4_3:-71997234 None:intergenic
GTTGGTAATAATTGGCTGAG+AGG 0.708552 4_3:-71997622 None:intergenic
ATTATGACTTGAGAATGCTG+AGG 0.735899 4_3:-71997778 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATAAACAAAATAAAAT+AGG - chr4_3:71998178-71998197 None:intergenic 10.0%
!! GAACAAAAGAAATAAAAAAA+AGG - chr4_3:71993629-71993648 None:intergenic 15.0%
!!! GTTTCTATTATTTTATCTTT+AGG + chr4_3:71993904-71993923 Msa0660870:intron 15.0%
!!! TAATTTTGACAAAAATTACA+CGG - chr4_3:71994174-71994193 None:intergenic 15.0%
!!! TTTGTTTTAATATTACTTTC+AGG + chr4_3:71995112-71995131 Msa0660870:intron 15.0%
!! ACAATTGAAAATAGTTTATA+TGG - chr4_3:71996636-71996655 None:intergenic 15.0%
!! TATAAATAATTAAGTTCCTA+AGG + chr4_3:71996772-71996791 Msa0660870:intron 15.0%
!!! GAAAAAATAAGAGGTATTAT+TGG - chr4_3:71993520-71993539 None:intergenic 20.0%
!!! ATTATCCTTTTCAATTTTCA+TGG + chr4_3:71993560-71993579 Msa0660870:five_prime_UTR 20.0%
!! GTTCAATGAAATTTGTATAT+TGG - chr4_3:71993691-71993710 None:intergenic 20.0%
!! ATCAACTTTGATTAATCAAA+TGG + chr4_3:71994023-71994042 Msa0660870:intron 20.0%
!!! TGTAACTTTTGTCAAAATTA+CGG + chr4_3:71994140-71994159 Msa0660870:intron 20.0%
!!! TAATTTTTGTCAAAATTACG+TGG + chr4_3:71994176-71994195 Msa0660870:intron 20.0%
!!! AATTTATTTTGGAATACTCT+TGG - chr4_3:71994384-71994403 None:intergenic 20.0%
!! AACAAAATAATGAAATGCAA+TGG - chr4_3:71994597-71994616 None:intergenic 20.0%
!!! TATTTTGTTCATCATATCAT+AGG + chr4_3:71994608-71994627 Msa0660870:intron 20.0%
!!! ATATAGTTTGTTAGTTAGAT+TGG - chr4_3:71994667-71994686 None:intergenic 20.0%
!! TTACATAAACTCTTATAACT+TGG + chr4_3:71994724-71994743 Msa0660870:intron 20.0%
!! CATACATAATATATATCAAC+AGG + chr4_3:71995073-71995092 Msa0660870:intron 20.0%
!! TATATGTCCAAAAACAAAAA+AGG + chr4_3:71995968-71995987 Msa0660870:CDS 20.0%
!!! ACTATTTTCAATTGTAAATG+TGG + chr4_3:71996641-71996660 Msa0660870:intron 20.0%
!! AAATTTATAATACCAAAAGC+AGG - chr4_3:71996940-71996959 None:intergenic 20.0%
!!! TGAAAAAAGGTAACAATTTT+GGG - chr4_3:71997263-71997282 None:intergenic 20.0%
!!! TTGAAAAAAGGTAACAATTT+TGG - chr4_3:71997264-71997283 None:intergenic 20.0%
!! TAGCAGATATGATTTATATA+TGG + chr4_3:71997870-71997889 Msa0660870:three_prime_UTR 20.0%
!! TTTAGTAATATTCTCAAGAA+AGG - chr4_3:71997938-71997957 None:intergenic 20.0%
!! ATTGTAATTGTATTGTATAG+TGG + chr4_3:71998037-71998056 Msa0660870:three_prime_UTR 20.0%
!! AACAAAATAAAATAGGAAAC+TGG - chr4_3:71998171-71998190 None:intergenic 20.0%
!!! GATATCTGTTTTTACAATTA+AGG - chr4_3:71998235-71998254 None:intergenic 20.0%
!! TTATTGGTTAAAGTATGTGA+TGG - chr4_3:71993504-71993523 None:intergenic 25.0%
! TGTAGTCATGAAAAAATAAG+AGG - chr4_3:71993529-71993548 None:intergenic 25.0%
! CTTCTTAGTAGTAAAATCAA+TGG + chr4_3:71993732-71993751 Msa0660870:five_prime_UTR 25.0%
! TTCTTCAATAGTAGTCTAAT+TGG + chr4_3:71993828-71993847 Msa0660870:five_prime_UTR 25.0%
!!! AATAGAAACTTTTGAGAGTA+AGG - chr4_3:71993893-71993912 None:intergenic 25.0%
!!! ATCAAAGTTGATAAATTCAG+AGG - chr4_3:71994015-71994034 None:intergenic 25.0%
! TAGTGAGTTTGACAAAATTT+AGG + chr4_3:71994088-71994107 Msa0660870:intron 25.0%
!! TGATAATTTGCCATATATCA+CGG - chr4_3:71994205-71994224 None:intergenic 25.0%
!! AGAGAAGGACAAATTTATTT+TGG - chr4_3:71994395-71994414 None:intergenic 25.0%
! TTGTTCTTTCATTACAGTTA+AGG + chr4_3:71994423-71994442 Msa0660870:intron 25.0%
! TTTATTTATGTGTACCTGAA+AGG - chr4_3:71994572-71994591 None:intergenic 25.0%
! CGGAATTTATGTTAATTAAC+AGG + chr4_3:71994772-71994791 Msa0660870:intron 25.0%
! CATAGAATTGATGAAAATAG+TGG + chr4_3:71994811-71994830 Msa0660870:CDS 25.0%
!!! AGTTTGGATTAACTTGTTTA+TGG - chr4_3:71995341-71995360 None:intergenic 25.0%
! ACTGAATATAACATCTCAAA+AGG + chr4_3:71995542-71995561 Msa0660870:CDS 25.0%
!! AAGAAGTTTCTAGATAAAGT+TGG + chr4_3:71995591-71995610 Msa0660870:CDS 25.0%
! ATGTATCTAATGAAAAAGCA+TGG + chr4_3:71995654-71995673 Msa0660870:CDS 25.0%
!!! ATAAGAAAGTGGATTAATGA+GGG + chr4_3:71995939-71995958 Msa0660870:CDS 25.0%
!! TACAGAAGATTTTATGTTGT+TGG + chr4_3:71996091-71996110 Msa0660870:CDS 25.0%
! ACCATACAAAGATCAAAATT+GGG - chr4_3:71996586-71996605 None:intergenic 25.0%
!! TCTACAATTTCAGAAACTTT+TGG - chr4_3:71996727-71996746 None:intergenic 25.0%
!!! AAGTTTCTGAAATTGTAGAA+AGG + chr4_3:71996728-71996747 Msa0660870:intron 25.0%
!!! TGTGTGTAATTTTTCAGAAA+TGG + chr4_3:71996973-71996992 Msa0660870:intron 25.0%
!!! GTAATTTTTCAGAAATGGTA+TGG + chr4_3:71996978-71996997 Msa0660870:intron 25.0%
! TTACTTGATTCTGATAACAA+AGG + chr4_3:71997153-71997172 Msa0660870:CDS 25.0%
! TACTTGATTCTGATAACAAA+GGG + chr4_3:71997154-71997173 Msa0660870:CDS 25.0%
! TTATTCTTATAGCATGTTGT+TGG - chr4_3:71997182-71997201 None:intergenic 25.0%
!!! GGATTATCTTAAGTTGATTT+TGG + chr4_3:71997476-71997495 Msa0660870:CDS 25.0%
!!! TTTGATGATCTATTTTCTTC+AGG - chr4_3:71997675-71997694 None:intergenic 25.0%
! GATTTTACTACTAAGAAGCA+AGG - chr4_3:71993730-71993749 None:intergenic 30.0%
! CCAAACTAATAAAGTGCTAT+TGG - chr4_3:71993786-71993805 None:intergenic 30.0%
CCAATAGCACTTTATTAGTT+TGG + chr4_3:71993783-71993802 Msa0660870:five_prime_UTR 30.0%
CAATAGTAACGTGTAATTCT+AGG + chr4_3:71993856-71993875 Msa0660870:five_prime_UTR 30.0%
TTCAAAGTTCAAACTGTGTT+TGG + chr4_3:71993927-71993946 Msa0660870:intron 30.0%
TCAGAGGTTAACAATTTGAT+TGG - chr4_3:71993999-71994018 None:intergenic 30.0%
! CAAATGGCTTACGATTTTAT+TGG + chr4_3:71994039-71994058 Msa0660870:intron 30.0%
TTTGTAGCTTCACAAAATCA+TGG - chr4_3:71994119-71994138 None:intergenic 30.0%
! AACTTTTGTCAAAATTACGG+TGG + chr4_3:71994143-71994162 Msa0660870:intron 30.0%
! GATAATTTGCCATATATCAC+GGG - chr4_3:71994204-71994223 None:intergenic 30.0%
AGAACAAAATCTACTAGAGA+AGG - chr4_3:71994410-71994429 None:intergenic 30.0%
TATGTGAATGAACGACAAAT+CGG - chr4_3:71994701-71994720 None:intergenic 30.0%
TAACTTGGATGTTAGTACTA+AGG + chr4_3:71994739-71994758 Msa0660870:intron 30.0%
AGTACTAAGGATTCTATTGA+CGG + chr4_3:71994752-71994771 Msa0660870:intron 30.0%
ATTACTTCTTCAACATTGCT+CGG + chr4_3:71994937-71994956 Msa0660870:CDS 30.0%
AGAAAAAAAAAAGACACACC+AGG - chr4_3:71994979-71994998 None:intergenic 30.0%
!!! CTAATGACTTTTTCGCTTTT+TGG - chr4_3:71995169-71995188 None:intergenic 30.0%
AGAAAGGATAAAACTCAGTT+TGG - chr4_3:71995357-71995376 None:intergenic 30.0%
TGAGATGTTATATTCAGTCT+TGG - chr4_3:71995540-71995559 None:intergenic 30.0%
AGATCGTTTCTCGATTAAAA+TGG - chr4_3:71995568-71995587 None:intergenic 30.0%
! TCAAGATTGTCTTCTTTTTC+GGG - chr4_3:71995626-71995645 None:intergenic 30.0%
! CTCAAGATTGTCTTCTTTTT+CGG - chr4_3:71995627-71995646 None:intergenic 30.0%
AAAGCATGGAAACTTAATGT+TGG + chr4_3:71995668-71995687 Msa0660870:CDS 30.0%
TAAAACTGAGATGAATCAGT+CGG + chr4_3:71995815-71995834 Msa0660870:CDS 30.0%
GAGTAACATATGAAAGAATG+TGG + chr4_3:71995916-71995935 Msa0660870:CDS 30.0%
AAAGAATGTGGATAAGAAAG+TGG + chr4_3:71995928-71995947 Msa0660870:CDS 30.0%
!! GATAAGAAAGTGGATTAATG+AGG + chr4_3:71995938-71995957 Msa0660870:CDS 30.0%
!!! GGTAGTTCCTTTTTTGTTTT+TGG - chr4_3:71995978-71995997 None:intergenic 30.0%
AAGTCCTTCATGACAATATT+TGG - chr4_3:71996017-71996036 None:intergenic 30.0%
TCAAAGTGGTGCAAAAATAA+TGG + chr4_3:71996041-71996060 Msa0660870:CDS 30.0%
ATAACTTCCCAAACTCTTAA+TGG - chr4_3:71996132-71996151 None:intergenic 30.0%
TCAACTTCATGATCTTCAAT+GGG + chr4_3:71996349-71996368 Msa0660870:CDS 30.0%
CACCATACAAAGATCAAAAT+TGG - chr4_3:71996587-71996606 None:intergenic 30.0%
! ACTTTTGGATGATTCACTTT+AGG - chr4_3:71996712-71996731 None:intergenic 30.0%
CAAGAAACTTAAAATTTGCG+TGG - chr4_3:71996876-71996895 None:intergenic 30.0%
! AATCAAGCAAACGAATTTTC+AGG - chr4_3:71996903-71996922 None:intergenic 30.0%
TTATGAACGTATCTTCACTT+AGG - chr4_3:71997008-71997027 None:intergenic 30.0%
TGAAGATACGTTCATAAAGT+TGG + chr4_3:71997011-71997030 Msa0660870:CDS 30.0%
AACATTTGTGATACTGAAGA+AGG + chr4_3:71997324-71997343 Msa0660870:CDS 30.0%
AAATTCTATTGCCGAAACAA+AGG + chr4_3:71997350-71997369 Msa0660870:CDS 30.0%
AATTCTATTGCCGAAACAAA+GGG + chr4_3:71997351-71997370 Msa0660870:CDS 30.0%
ATGTACATGTTCTCAAAATG+TGG + chr4_3:71997455-71997474 Msa0660870:CDS 30.0%
TTCTGTGAGATATAGAAAAG+AGG - chr4_3:71997519-71997538 None:intergenic 30.0%
! CTTTTCTATATCTCACAGAA+TGG + chr4_3:71997519-71997538 Msa0660870:CDS 30.0%
TCACAGAATGGAAATGATAA+AGG + chr4_3:71997531-71997550 Msa0660870:CDS 30.0%
GAATGGAAATGATAAAGGAA+AGG + chr4_3:71997536-71997555 Msa0660870:CDS 30.0%
CAAAATATGAAGCAATTCCA+CGG - chr4_3:71997568-71997587 None:intergenic 30.0%
AATGTGTTGAGAATGAAGAT+AGG + chr4_3:71997598-71997617 Msa0660870:CDS 30.0%
!! GAACTGTTGTTGGTAATAAT+TGG - chr4_3:71997633-71997652 None:intergenic 30.0%
AATCCTAGTTTCTTCAGAAA+TGG - chr4_3:71997705-71997724 None:intergenic 30.0%
! GAAATTCATTTTCACCTTCT+GGG - chr4_3:71997759-71997778 None:intergenic 30.0%
TGTGAATGTGTGTGATATAT+TGG + chr4_3:71997907-71997926 Msa0660870:three_prime_UTR 30.0%
!! ATACATCTGCTGCTTAATTT+TGG + chr4_3:71997999-71998018 Msa0660870:three_prime_UTR 30.0%
GTAATTGTATTGTATAGTGG+TGG + chr4_3:71998040-71998059 Msa0660870:three_prime_UTR 30.0%
! GGTTAAAGTATGTGATGGAA+GGG - chr4_3:71993499-71993518 None:intergenic 35.0%
! TGGTTAAAGTATGTGATGGA+AGG - chr4_3:71993500-71993519 None:intergenic 35.0%
GCATGCCATGAAAATTGAAA+AGG - chr4_3:71993568-71993587 None:intergenic 35.0%
TTTCAAGCTCATGAGATTCT+TGG - chr4_3:71993651-71993670 None:intergenic 35.0%
GCAAGGTGAACAAAAAACAA+CGG - chr4_3:71993713-71993732 None:intergenic 35.0%
TTGGAAGTTGCTTGCATATT+TGG + chr4_3:71993802-71993821 Msa0660870:five_prime_UTR 35.0%
GGTTAACAATTTGATTGGAG+AGG - chr4_3:71993994-71994013 None:intergenic 35.0%
GAGAGACTTATAACCATGTT+TGG + chr4_3:71994061-71994080 Msa0660870:intron 35.0%
AACTCACTATGATCCAAACA+TGG - chr4_3:71994077-71994096 None:intergenic 35.0%
CTAAATTGAGAAGTGAGAAG+AGG - chr4_3:71994328-71994347 None:intergenic 35.0%
CTCTTCTCACTTCTCAATTT+AGG + chr4_3:71994326-71994345 Msa0660870:intron 35.0%
!! CACTATTGGTTCTCATTAAG+AGG - chr4_3:71994516-71994535 None:intergenic 35.0%
CTCTTAATGAGAACCAATAG+TGG + chr4_3:71994514-71994533 Msa0660870:exon 35.0%
AACCAATAGTGGTGAAAAAG+TGG + chr4_3:71994525-71994544 Msa0660870:CDS 35.0%
GAGAATATTTCCAGACATAG+AGG + chr4_3:71994844-71994863 Msa0660870:CDS 35.0%
TCTATTCCTGATGAACATCT+TGG - chr4_3:71994878-71994897 None:intergenic 35.0%
ACAAAGCTTAATTCCCATTC+AGG + chr4_3:71995190-71995209 Msa0660870:CDS 35.0%
CAAAGCTTAATTCCCATTCA+GGG + chr4_3:71995191-71995210 Msa0660870:CDS 35.0%
!! AGTTTTATCCTTTCTGTTGC+AGG + chr4_3:71995362-71995381 Msa0660870:intron 35.0%
TGAGTATGCTACATTTGTTC+TGG + chr4_3:71995407-71995426 Msa0660870:CDS 35.0%
GTAAGAGAATTGCTTCATCA+AGG + chr4_3:71995459-71995478 Msa0660870:CDS 35.0%
! TTTAACTTCAAAGTCGTCCA+TGG - chr4_3:71995499-71995518 None:intergenic 35.0%
CATGGAAACTTAATGTTGGT+GGG + chr4_3:71995672-71995691 Msa0660870:CDS 35.0%
TAGAGAAGAACCAATTGCAA+GGG + chr4_3:71995722-71995741 Msa0660870:CDS 35.0%
TACATCAACCTTGAGGATAT+TGG - chr4_3:71995873-71995892 None:intergenic 35.0%
! TTTGGAGTCAATGTCATATG+AGG - chr4_3:71995999-71996018 None:intergenic 35.0%
GACTCCAAATATTGTCATGA+AGG + chr4_3:71996010-71996029 Msa0660870:CDS 35.0%
TGAAGGACTTGATATCAAAG+TGG + chr4_3:71996027-71996046 Msa0660870:CDS 35.0%
AAATCTTCTGTATGTCTACC+TGG - chr4_3:71996083-71996102 None:intergenic 35.0%
AATCACCTCCATTAAGAGTT+TGG + chr4_3:71996121-71996140 Msa0660870:CDS 35.0%
ATCACCTCCATTAAGAGTTT+GGG + chr4_3:71996122-71996141 Msa0660870:CDS 35.0%
CATCAGATTCAAGTCATGAT+TGG - chr4_3:71996246-71996265 None:intergenic 35.0%
CATTGAAGATCATGAAGTTG+AGG - chr4_3:71996350-71996369 None:intergenic 35.0%
CTCAACTTCATGATCTTCAA+TGG + chr4_3:71996348-71996367 Msa0660870:CDS 35.0%
TTGCTCAAAAATCTGACTAC+TGG - chr4_3:71996467-71996486 None:intergenic 35.0%
CAAAGATCAAAATTGGGCAA+GGG - chr4_3:71996580-71996599 None:intergenic 35.0%
ACAAAGATCAAAATTGGGCA+AGG - chr4_3:71996581-71996600 None:intergenic 35.0%
!! GCCCAATTTTGATCTTTGTA+TGG + chr4_3:71996582-71996601 Msa0660870:intron 35.0%
ATCCTCAATCATATCCATGT+CGG - chr4_3:71996802-71996821 None:intergenic 35.0%
AAAATTTGCGTGGATCAACT+TGG - chr4_3:71996866-71996885 None:intergenic 35.0%
! TTTATCTTTGCACCTGCTTT+TGG + chr4_3:71996925-71996944 Msa0660870:intron 35.0%
CTTCTTGATTCAGAAGTGAT+AGG + chr4_3:71997039-71997058 Msa0660870:CDS 35.0%
TCCAGAAAGTTCTTCCATTA+TGG - chr4_3:71997076-71997095 None:intergenic 35.0%
! ACAGCTCTGTCTTTGAAAAA+AGG - chr4_3:71997276-71997295 None:intergenic 35.0%
! ACTTATGCATCCCTTTGTTT+CGG - chr4_3:71997364-71997383 None:intergenic 35.0%
ATTGCTGAAATACTTGAGAG+TGG + chr4_3:71997387-71997406 Msa0660870:CDS 35.0%
! ATTTTCTTCAGGATGACTCT+TGG - chr4_3:71997664-71997683 None:intergenic 35.0%
! TCACCATTTCTGAAGAAACT+AGG + chr4_3:71997699-71997718 Msa0660870:CDS 35.0%
! GGAAATTCATTTTCACCTTC+TGG - chr4_3:71997760-71997779 None:intergenic 35.0%
ATTATGACTTGAGAATGCTG+AGG - chr4_3:71997781-71997800 None:intergenic 35.0%
ATATCCTTGTGATACCATTG+TGG + chr4_3:71997799-71997818 Msa0660870:three_prime_UTR 35.0%
TATCCTTGTGATACCATTGT+GGG + chr4_3:71997800-71997819 Msa0660870:three_prime_UTR 35.0%
ACACACATTCACAAAGATTG+AGG - chr4_3:71997900-71997919 None:intergenic 35.0%
! TAATTTTGGACTGTAACAGC+AGG + chr4_3:71998013-71998032 Msa0660870:three_prime_UTR 35.0%
ATTGTATTGTATAGTGGTGG+TGG + chr4_3:71998043-71998062 Msa0660870:three_prime_UTR 35.0%
GAAGTTGCTTGCATATTTGG+TGG + chr4_3:71993805-71993824 Msa0660870:five_prime_UTR 40.0%
CTACAGCTACATATCAGACA+AGG - chr4_3:71994487-71994506 None:intergenic 40.0%
CTTGTCTGATATGTAGCTGT+AGG + chr4_3:71994485-71994504 Msa0660870:intron 40.0%
!!! CTCCACTTTTTCACCACTAT+TGG - chr4_3:71994530-71994549 None:intergenic 40.0%
TATGTGTACCTGAAAGGAGA+GGG - chr4_3:71994566-71994585 None:intergenic 40.0%
TTATGTGTACCTGAAAGGAG+AGG - chr4_3:71994567-71994586 None:intergenic 40.0%
TATTACTTTCAGGCTGTGAC+AGG + chr4_3:71995122-71995141 Msa0660870:intron 40.0%
CTTAATTCCCATTCAGGGTA+TGG + chr4_3:71995196-71995215 Msa0660870:CDS 40.0%
TCTAGAAACCTGCAACAGAA+AGG - chr4_3:71995373-71995392 None:intergenic 40.0%
!!! GTCTTCTTTTTCGGGTCATT+TGG - chr4_3:71995618-71995637 None:intergenic 40.0%
GCATGGAAACTTAATGTTGG+TGG + chr4_3:71995671-71995690 Msa0660870:CDS 40.0%
GTAGAGAAGAACCAATTGCA+AGG + chr4_3:71995721-71995740 Msa0660870:CDS 40.0%
CTAGAGTTATCCCTTGCAAT+TGG - chr4_3:71995735-71995754 None:intergenic 40.0%
GATTTCTTCGTCGAGAATCA+CGG - chr4_3:71995758-71995777 None:intergenic 40.0%
ATTGGACACTTGTATTCCTC+AGG - chr4_3:71995855-71995874 None:intergenic 40.0%
ACAAGTGTCCAATATCCTCA+AGG + chr4_3:71995862-71995881 Msa0660870:CDS 40.0%
AGGATCATACATCAACCTTG+AGG - chr4_3:71995880-71995899 None:intergenic 40.0%
GATCCTGTTATCATTGCTTC+TGG + chr4_3:71995894-71995913 Msa0660870:CDS 40.0%
ACTCCAGAAGCAATGATAAC+AGG - chr4_3:71995900-71995919 None:intergenic 40.0%
ACTTCCCAAACTCTTAATGG+AGG - chr4_3:71996129-71996148 None:intergenic 40.0%
CAATGATCTGAACTTGCCAA+TGG + chr4_3:71996154-71996173 Msa0660870:CDS 40.0%
! GACATGTTGGTAAGATCCAT+TGG - chr4_3:71996173-71996192 None:intergenic 40.0%
GATCTTACCAACATGTCACT+TGG + chr4_3:71996176-71996195 Msa0660870:CDS 40.0%
CAATGATCCAAGTGACATGT+TGG - chr4_3:71996186-71996205 None:intergenic 40.0%
! CAACATGTCACTTGGATCAT+TGG + chr4_3:71996184-71996203 Msa0660870:CDS 40.0%
!! GGTCTTAACTTGTGACACAT+CGG - chr4_3:71996225-71996244 None:intergenic 40.0%
!! AGGAGAAGTACTTGCCATAA+TGG + chr4_3:71997059-71997078 Msa0660870:CDS 40.0%
! GCCATAATGGAAGAACTTTC+TGG + chr4_3:71997072-71997091 Msa0660870:CDS 40.0%
! ATGGAAGAACTTTCTGGAGA+TGG + chr4_3:71997078-71997097 Msa0660870:CDS 40.0%
! TGCATTGGATGCTGCAATTT+TGG - chr4_3:71997115-71997134 None:intergenic 40.0%
TAAGACAGAAGTGAGTGCAT+TGG - chr4_3:71997130-71997149 None:intergenic 40.0%
TGGACAAACTTCACTGTTGT+AGG - chr4_3:71997217-71997236 None:intergenic 40.0%
CAATTGACAGATACCATGTG+TGG - chr4_3:71997237-71997256 None:intergenic 40.0%
!! GTTGGTAATAATTGGCTGAG+AGG - chr4_3:71997625-71997644 None:intergenic 40.0%
GGAATCTGTAGAACTGTTGT+TGG - chr4_3:71997643-71997662 None:intergenic 40.0%
ATCCTTGTGATACCATTGTG+GGG + chr4_3:71997801-71997820 Msa0660870:three_prime_UTR 40.0%
ATGCTTGATAAGAGCCAGTT+TGG + chr4_3:71997838-71997857 Msa0660870:three_prime_UTR 40.0%
TAAGAGCCAGTTTGGATTGA+TGG + chr4_3:71997846-71997865 Msa0660870:three_prime_UTR 40.0%
TGCTATCCATCAATCCAAAC+TGG - chr4_3:71997855-71997874 None:intergenic 40.0%
GTGGTGGTTATGTTTACAGT+AGG + chr4_3:71998065-71998084 Msa0660870:three_prime_UTR 40.0%
! GAAACTGGTCTCTAGTATGT+TGG - chr4_3:71998156-71998175 None:intergenic 40.0%
TACGTGGTTCCCGTGATATA+TGG + chr4_3:71994192-71994211 Msa0660870:intron 45.0%
TCCAAATACCCTCTCCTTTC+AGG + chr4_3:71994555-71994574 Msa0660870:CDS 45.0%
!! ACCTGAAAGGAGAGGGTATT+TGG - chr4_3:71994559-71994578 None:intergenic 45.0%
GGAATGCAGCAAACTTTACC+TGG + chr4_3:71994958-71994977 Msa0660870:CDS 45.0%
ACTGGAACCATACCCTGAAT+GGG - chr4_3:71995206-71995225 None:intergenic 45.0%
TCTGGATTCTGCTGAAGAAG+AGG + chr4_3:71995425-71995444 Msa0660870:CDS 45.0%
ATTCTGCTGAAGAAGAGGCT+GGG + chr4_3:71995430-71995449 Msa0660870:CDS 45.0%
AGGTTCAACCTCAGATTCCA+TGG + chr4_3:71995479-71995498 Msa0660870:CDS 45.0%
AAAGTCGTCCATGGAATCTG+AGG - chr4_3:71995490-71995509 None:intergenic 45.0%
! AATGTTGGTGGGAGAACACA+TGG + chr4_3:71995683-71995702 Msa0660870:CDS 45.0%
GATGAATCAGTCGGATAGAG+TGG + chr4_3:71995824-71995843 Msa0660870:CDS 45.0%
ATGTCTACCTGGATCAGGAA+TGG - chr4_3:71996072-71996091 None:intergenic 45.0%
TCTGTATGTCTACCTGGATC+AGG - chr4_3:71996077-71996096 None:intergenic 45.0%
GCATGAACTGTATGCTTGTG+AGG - chr4_3:71996296-71996315 None:intergenic 45.0%
CTGACACTGACAAAGTAGCT+TGG - chr4_3:71996427-71996446 None:intergenic 45.0%
GAGCAATGCAAATGATCAGC+AGG + chr4_3:71996481-71996500 Msa0660870:CDS 45.0%
CGGAATTTGTGAACAACTGC+AGG + chr4_3:71996540-71996559 Msa0660870:CDS 45.0%
TATCCATGTCGGACAACCTT+AGG - chr4_3:71996791-71996810 None:intergenic 45.0%
GTCCGACATGGATATGATTG+AGG + chr4_3:71996797-71996816 Msa0660870:intron 45.0%
CAGTGAAGTTTGTCCACACA+TGG + chr4_3:71997221-71997240 Msa0660870:CDS 45.0%
!! TAAAGGAAAGGAAAGTGCCG+TGG + chr4_3:71997548-71997567 Msa0660870:CDS 45.0%
!! CTTCTGGGTTTTGATGCATG+TGG - chr4_3:71997744-71997763 None:intergenic 45.0%
CACATGCATCAAAACCCAGA+AGG + chr4_3:71997742-71997761 Msa0660870:CDS 45.0%
TCCTTGTGATACCATTGTGG+GGG + chr4_3:71997802-71997821 Msa0660870:three_prime_UTR 45.0%
GATATGTATTCCCCCCACAA+TGG - chr4_3:71997816-71997835 None:intergenic 45.0%
!! GTATTGTATAGTGGTGGTGG+TGG + chr4_3:71998046-71998065 Msa0660870:three_prime_UTR 45.0%
! ACAGTAGGCACTGTAACAGT+AGG + chr4_3:71998080-71998099 Msa0660870:three_prime_UTR 45.0%
! CAGTAGGCACTGTAACAGTA+GGG + chr4_3:71998081-71998100 Msa0660870:three_prime_UTR 45.0%
CTAGTATGTTGGCAGGAAGA+GGG - chr4_3:71998145-71998164 None:intergenic 45.0%
TCTAGTATGTTGGCAGGAAG+AGG - chr4_3:71998146-71998165 None:intergenic 45.0%
GCACGTCCAAGATGTTCATC+AGG + chr4_3:71994869-71994888 Msa0660870:CDS 50.0%
GACTGGAACCATACCCTGAA+TGG - chr4_3:71995207-71995226 None:intergenic 50.0%
GGGTATGGTTCCAGTCATGT+TGG + chr4_3:71995211-71995230 Msa0660870:CDS 50.0%
! TCCAGTCATGTTGGCTGTAG+AGG + chr4_3:71995220-71995239 Msa0660870:CDS 50.0%
ACCTCTACAGCCAACATGAC+TGG - chr4_3:71995224-71995243 None:intergenic 50.0%
GATTCTGCTGAAGAAGAGGC+TGG + chr4_3:71995429-71995448 Msa0660870:CDS 50.0%
GGACACTTGTATTCCTCAGG+TGG - chr4_3:71995852-71995871 None:intergenic 50.0%
TTCCGACAGAAGCTGAGTTC+CGG - chr4_3:71996525-71996544 None:intergenic 50.0%
GTTCCTAAGGTTGTCCGACA+TGG + chr4_3:71996785-71996804 Msa0660870:intron 50.0%
TGCGTGGATCAACTTGGTTG+CGG - chr4_3:71996860-71996879 None:intergenic 50.0%
CCTTGTGATACCATTGTGGG+GGG + chr4_3:71997803-71997822 Msa0660870:three_prime_UTR 50.0%
!! TTGTATAGTGGTGGTGGTGG+TGG + chr4_3:71998049-71998068 Msa0660870:three_prime_UTR 50.0%
GTTGGCAGGAAGAGGGAAAA+GGG - chr4_3:71998138-71998157 None:intergenic 50.0%
TGTTGGCAGGAAGAGGGAAA+AGG - chr4_3:71998139-71998158 None:intergenic 50.0%
CTGGTCTCTAGTATGTTGGC+AGG - chr4_3:71998152-71998171 None:intergenic 50.0%
GGACGTGCTTCCTCTATGTC+TGG - chr4_3:71994857-71994876 None:intergenic 55.0%
GGTGTCTCCATTCCTGATCC+AGG + chr4_3:71996062-71996081 Msa0660870:CDS 55.0%
!! GACGTAAAAGCGCTGAGAGC+CGG + chr4_3:71996503-71996522 Msa0660870:CDS 55.0%
AGCCGGAACTCAGCTTCTGT+CGG + chr4_3:71996520-71996539 Msa0660870:CDS 55.0%
CCCCCCACAATGGTATCACA+AGG - chr4_3:71997806-71997825 None:intergenic 55.0%
GGATAGAGTGGCACCACCTG+AGG + chr4_3:71995836-71995855 Msa0660870:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 71993491 71998292 71993491 ID=Msa0660870;Name=Msa0660870
chr4_3 mRNA 71993491 71998292 71993491 ID=Msa0660870-mRNA-1;Parent=Msa0660870;Name=Msa0660870-mRNA-1;_AED=0.03;_eAED=0.03;_QI=406|1|1|1|0.8|0.83|6|525|768
chr4_3 exon 71993491 71993877 71993491 ID=Msa0660870-mRNA-1:exon:21558;Parent=Msa0660870-mRNA-1
chr4_3 exon 71994501 71994576 71994501 ID=Msa0660870-mRNA-1:exon:21559;Parent=Msa0660870-mRNA-1
chr4_3 exon 71994794 71994979 71994794 ID=Msa0660870-mRNA-1:exon:21560;Parent=Msa0660870-mRNA-1
chr4_3 exon 71995134 71995241 71995134 ID=Msa0660870-mRNA-1:exon:21561;Parent=Msa0660870-mRNA-1
chr4_3 exon 71995384 71996561 71995384 ID=Msa0660870-mRNA-1:exon:21562;Parent=Msa0660870-mRNA-1
chr4_3 exon 71996990 71998292 71996990 ID=Msa0660870-mRNA-1:exon:21563;Parent=Msa0660870-mRNA-1
chr4_3 five_prime_UTR 71993491 71993877 71993491 ID=Msa0660870-mRNA-1:five_prime_utr;Parent=Msa0660870-mRNA-1
chr4_3 five_prime_UTR 71994501 71994519 71994501 ID=Msa0660870-mRNA-1:five_prime_utr;Parent=Msa0660870-mRNA-1
chr4_3 CDS 71994520 71994576 71994520 ID=Msa0660870-mRNA-1:cds;Parent=Msa0660870-mRNA-1
chr4_3 CDS 71994794 71994979 71994794 ID=Msa0660870-mRNA-1:cds;Parent=Msa0660870-mRNA-1
chr4_3 CDS 71995134 71995241 71995134 ID=Msa0660870-mRNA-1:cds;Parent=Msa0660870-mRNA-1
chr4_3 CDS 71995384 71996561 71995384 ID=Msa0660870-mRNA-1:cds;Parent=Msa0660870-mRNA-1
chr4_3 CDS 71996990 71997767 71996990 ID=Msa0660870-mRNA-1:cds;Parent=Msa0660870-mRNA-1
chr4_3 three_prime_UTR 71997768 71998292 71997768 ID=Msa0660870-mRNA-1:three_prime_utr;Parent=Msa0660870-mRNA-1
Gene Sequence

>Msa0660870

ATGAGAACCAATAGTGGTGAAAAAGTGGAGACTTTTCCAAATACCCTCTCCTTTCAGGTACATAGTAAAATATGCATAGAATTGATGAAAATAGTGGACAGCATCATGAGAATATTTCCAGACATAGAGGAAGCACGTCCAAGATGTTCATCAGGAATAGAGTCTCTATGTTTTCTGAACAACTCAATTGAGAAAGCTAAATTACTTCTTCAACATTGCTCGGAATGCAGCAAACTTTACCTGGCTGTGACAGGAGAAACAGTACTTTCGAGATGCCAAAAAGCGAAAAAGTCATTAGAACAAAGCTTAATTCCCATTCAGGGTATGGTTCCAGTCATGTTGGCTGTAGAGGTTTCTAGAATAATTGATGATCTTGAGTATGCTACATTTGTTCTGGATTCTGCTGAAGAAGAGGCTGGGAGAGTTGTAAGAGAATTGCTTCATCAAGGTTCAACCTCAGATTCCATGGACGACTTTGAAGTTAAAGCTCTTCAGTTTGCAGCACCAAGACTGAATATAACATCTCAAAAGGCCATTTTAATCGAGAAACGATCTATCAAGAAGTTTCTAGATAAAGTTGGACCAAATGACCCGAAAAAGAAGACAATCTTGAGATATCTTATGTATCTAATGAAAAAGCATGGAAACTTAATGTTGGTGGGAGAACACATGGATAATTTTTATTCTCGTAGAGAAGAACCAATTGCAAGGGATAACTCTAGCCGTGATTCTCGACGAAGAAATCGTGTTGAGTCAGATCATAGCATGAACTATGATCAGCATAAAACTGAGATGAATCAGTCGGATAGAGTGGCACCACCTGAGGAATACAAGTGTCCAATATCCTCAAGGTTGATGTATGATCCTGTTATCATTGCTTCTGGAGTAACATATGAAAGAATGTGGATAAGAAAGTGGATTAATGAGGGTAAAACTATATGTCCAAAAACAAAAAAGGAACTACCTCATATGACATTGACTCCAAATATTGTCATGAAGGACTTGATATCAAAGTGGTGCAAAAATAATGGTGTCTCCATTCCTGATCCAGGTAGACATACAGAAGATTTTATGTTGTTGGATGCTTCAATCACCTCCATTAAGAGTTTGGGAAGTTATTTCAATGATCTGAACTTGCCAATGGATCTTACCAACATGTCACTTGGATCATTGGATAATAGTTTTAGTTCCGATGTGTCACAAGTTAAGACCAATCATGACTTGAATCTGATGATGATCAAGAGTAATGAAAATTCTCAGCCTCACAAGCATACAGTTCATGCAGAGATACATGATACAGATTTGATGCTCTTGCCTCAACTTCATGATCTTCAATGGGATTCTCAATGTAAGATCATTGAAGATTTGAAAGATCATATGAAAAGCAATAGCCAAGCTACTTTGTCAGTGTCAGCTGAAAATTTAGTTGAACCAGTAGTCAGATTTTTGAGCAATGCAAATGATCAGCAGGACGTAAAAGCGCTGAGAGCCGGAACTCAGCTTCTGTCGGAATTTGTGAACAACTGCAGAAATGGTATGGCTAACCTAAGTGAAGATACGTTCATAAAGTTGGCAAATCTTCTTGATTCAGAAGTGATAGGAGAAGTACTTGCCATAATGGAAGAACTTTCTGGAGATGGAAATAGTAAAGCCAAAATTGCAGCATCCAATGCACTCACTTCTGTCTTAAAGTTACTTGATTCTGATAACAAAGGGTTCCAACAACATGCTATAAGAATAATCTACAATTTGTCCTACAACAGTGAAGTTTGTCCACACATGGTATCTGTCAATTGCATCCCAAAATTGTTACCTTTTTTCAAAGACAGAGCTGTTTTGAGATACTGCATATATATATTGAAAAACATTTGTGATACTGAAGAAGGTAGAAATTCTATTGCCGAAACAAAGGGATGCATAAGTTCTATTGCTGAAATACTTGAGAGTGGAAGTAATGAAGAGCAAGAACATGCACTTGATGTTCTACTATCTTTATGTACATGTTCTCAAAATGTGGATTATCTTAAGTTGATTTTGGATGAAGATATTATCACTCCTCTTTTCTATATCTCACAGAATGGAAATGATAAAGGAAAGGAAAGTGCCGTGGAATTGCTTCATATTTTGAGAGATGCAAAATGTGTTGAGAATGAAGATAGGTCCTCTCAGCCAATTATTACCAACAACAGTTCTACAGATTCCAAGAGTCATCCTGAAGAAAATAGATCATCAAAGAAATCACCATTTCTGAAGAAACTAGGATTGTTCTCGAAATCTACTCCACATGCATCAAAACCCAGAAGGTGA
Protein sequence

>Msa0660870

MRTNSGEKVETFPNTLSFQVHSKICIELMKIVDSIMRIFPDIEEARPRCSSGIESLCFLNNSIEKAKLLLQHCSECSKLYLAVTGETVLSRCQKAKKSLEQSLIPIQGMVPVMLAVEVSRIIDDLEYATFVLDSAEEEAGRVVRELLHQGSTSDSMDDFEVKALQFAAPRLNITSQKAILIEKRSIKKFLDKVGPNDPKKKTILRYLMYLMKKHGNLMLVGEHMDNFYSRREEPIARDNSSRDSRRRNRVESDHSMNYDQHKTEMNQSDRVAPPEEYKCPISSRLMYDPVIIASGVTYERMWIRKWINEGKTICPKTKKELPHMTLTPNIVMKDLISKWCKNNGVSIPDPGRHTEDFMLLDASITSIKSLGSYFNDLNLPMDLTNMSLGSLDNSFSSDVSQVKTNHDLNLMMIKSNENSQPHKHTVHAEIHDTDLMLLPQLHDLQWDSQCKIIEDLKDHMKSNSQATLSVSAENLVEPVVRFLSNANDQQDVKALRAGTQLLSEFVNNCRNGMANLSEDTFIKLANLLDSEVIGEVLAIMEELSGDGNSKAKIAASNALTSVLKLLDSDNKGFQQHAIRIIYNLSYNSEVCPHMVSVNCIPKLLPFFKDRAVLRYCIYILKNICDTEEGRNSIAETKGCISSIAEILESGSNEEQEHALDVLLSLCTCSQNVDYLKLILDEDIITPLFYISQNGNDKGKESAVELLHILRDAKCVENEDRSSQPIITNNSSTDSKSHPEENRSSKKSPFLKKLGLFSKSTPHASKPRR*