AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0844500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAAACCTTCTCGTTTGA+TGG 0.254445 5_4:-22968768 Msa0844490:five_prime_UTR
GAAGAAGCCTTCTCGTTTGA+TGG 0.255371 5_4:-22964987 Msa0844490:five_prime_UTR
AGAAGTAATTAAATTTGTTC+TGG 0.261057 5_4:-22964914 Msa0844490:five_prime_UTR
GAATCAGATGCCTGGAGTTT+TGG 0.291750 5_4:-22964867 Msa0844490:five_prime_UTR
TTCACAGGTTCCCAGGCCTC+TGG 0.349601 5_4:+22964936 Msa0844500:CDS
TTCACAGGTTCCCAGGCCTC+TGG 0.349601 5_4:+22968717 Msa0844500:intron
TTAAATTTGTTCTGGGATGA+TGG 0.356464 5_4:-22964906 Msa0844490:five_prime_UTR
AAACTCCAGGCATCTGATTC+GGG 0.384582 5_4:+22964870 Msa0844500:CDS
AAAACTCCAGGCATCTGATT+CGG 0.391352 5_4:+22964869 Msa0844500:CDS
GAAGTAATTAAATTTGTTCT+GGG 0.407808 5_4:-22964913 Msa0844490:five_prime_UTR
CATGCCGTGGTTTCTGATAA+TGG 0.419747 5_4:-22968806 Msa0844490:five_prime_UTR
TTCTCGTTTGATGGATTGAC+CGG 0.450419 5_4:-22968759 Msa0844490:five_prime_UTR
TTCTCGTTTGATGGATTGAC+CGG 0.450728 5_4:-22964978 Msa0844490:five_prime_UTR
TCCACTAAGATCCAGAGGCC+TGG 0.472785 5_4:-22964947 Msa0844490:five_prime_UTR
TTCACTAAGATCCAGAGGCC+TGG 0.472785 5_4:-22968728 Msa0844490:five_prime_UTR
TTACTTCTTCACAGGTTCCC+AGG 0.474453 5_4:+22964929 Msa0844500:CDS
TTACTTCTTCACAGGTTCCC+AGG 0.474453 5_4:+22968710 Msa0844500:intron
AAATTTAATTACTTCTTCAC+AGG 0.476712 5_4:+22964921 Msa0844500:CDS
CCACTAAGATCCAGAGGCCT+GGG 0.480126 5_4:-22964946 Msa0844490:five_prime_UTR
TAAAGATGCAATGTAGAGAA+TGG 0.485247 5_4:+22964815 None:intergenic
TCACTAAGATCCAGAGGCCT+GGG 0.487028 5_4:-22968727 Msa0844490:five_prime_UTR
TATCTCCCGAATCAGATGCC+TGG 0.529832 5_4:-22964875 Msa0844490:five_prime_UTR
GGTTTCTTCAGAATTGTAGC+AGG 0.534349 5_4:+22968782 Msa0844500:CDS
GGCTTCTTCAGAATTGTAGC+AGG 0.536030 5_4:+22965001 Msa0844500:CDS
ATCTTAGTGGAATTGCACTC+CGG 0.577882 5_4:+22964959 Msa0844500:CDS
CGATGTACAACCAAAACTCC+AGG 0.579610 5_4:+22964857 Msa0844500:CDS
GGTCAATCCATCAAACGAGA+AGG 0.644748 5_4:+22964980 Msa0844500:CDS
GGTCAATCCATCAAACGAGA+AGG 0.647902 5_4:+22968761 Msa0844500:CDS
ATCTTAGTGAAATTGCACTC+CGG 0.655537 5_4:+22968740 Msa0844500:CDS
GCAATTCCACTAAGATCCAG+AGG 0.671623 5_4:-22964952 Msa0844490:five_prime_UTR
GCAATTTCACTAAGATCCAG+AGG 0.671623 5_4:-22968733 Msa0844490:five_prime_UTR
AGGTCCATTATCAGAAACCA+CGG 0.681132 5_4:+22968802 Msa0844500:CDS
TGATGCGAATGGTCATGCCG+TGG 0.694482 5_4:-22968819 Msa0844490:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATATTTTAAAAAATAAA+CGG - chr5_4:22966335-22966354 Msa0844490:intron 0.0%
!! TTTGAAAAACAAATATATAT+TGG - chr5_4:22967650-22967669 Msa0844490:five_prime_UTR 10.0%
!! GCATATATCTATATTATATA+TGG - chr5_4:22965509-22965528 Msa0844490:intron 15.0%
!! TATTCATAGATTATAAAATG+TGG - chr5_4:22966539-22966558 Msa0844490:intron 15.0%
!! TATTTAAATATCCTTGAAAT+GGG - chr5_4:22966616-22966635 Msa0844490:intron 15.0%
!!! AATCATCTATTTCATATTTT+GGG - chr5_4:22967059-22967078 Msa0844490:intron 15.0%
!! TATACTTCTTTATAATACAT+TGG + chr5_4:22967990-22968009 Msa0844500:intron 15.0%
!! TATAACATTATATTAGAACT+TGG + chr5_4:22968381-22968400 Msa0844500:intron 15.0%
!!! GAAGTAATTAAATTTGTTCT+GGG - chr5_4:22964916-22964935 Msa0844490:five_prime_UTR 20.0%
!!! AGAAGTAATTAAATTTGTTC+TGG - chr5_4:22964917-22964936 Msa0844490:five_prime_UTR 20.0%
!! AAATTTAATTACTTCTTCAC+AGG + chr5_4:22964921-22964940 Msa0844500:CDS 20.0%
!! AAATTTCAAAGTTACTATTG+TGG + chr5_4:22965151-22965170 Msa0844500:intron 20.0%
!! ATTTCTTATTCATGCAATAT+AGG - chr5_4:22965240-22965259 Msa0844490:intron 20.0%
!! ATTCTTTCAAAAAGAACTTA+GGG - chr5_4:22965384-22965403 Msa0844490:intron 20.0%
!! TATTCTTTCAAAAAGAACTT+AGG - chr5_4:22965385-22965404 Msa0844490:intron 20.0%
!!! CTCATTTGTTATAATATATC+TGG - chr5_4:22965425-22965444 Msa0844490:intron 20.0%
!! ATGAGACAATTATAACATAT+TGG + chr5_4:22965440-22965459 Msa0844500:intron 20.0%
!! TGAGACAATTATAACATATT+GGG + chr5_4:22965441-22965460 Msa0844500:intron 20.0%
!! TTCTATCATAAACCATATTT+GGG + chr5_4:22965601-22965620 Msa0844500:intron 20.0%
!! TATTAAAGAAACCATTAGAA+AGG - chr5_4:22965651-22965670 Msa0844490:intron 20.0%
!! TTGAGAAAATGATATACAAA+TGG - chr5_4:22966515-22966534 Msa0844490:intron 20.0%
!! CTATTTAAATATCCTTGAAA+TGG - chr5_4:22966617-22966636 Msa0844490:intron 20.0%
!! GAAACTAATATAACATATCA+TGG + chr5_4:22966669-22966688 Msa0844500:intron 20.0%
!!! TGAATCAGTTTTTTTTTCTA+GGG + chr5_4:22966888-22966907 Msa0844500:intron 20.0%
!! TCACTAATAGAAAATAAGAA+AGG + chr5_4:22967025-22967044 Msa0844500:intron 20.0%
!!! GAATCATCTATTTCATATTT+TGG - chr5_4:22967060-22967079 Msa0844490:intron 20.0%
!! GTATTTATAGCTAAATTTGA+AGG + chr5_4:22967249-22967268 Msa0844500:intron 20.0%
!! AGTCTATTACATTTACTTAT+TGG - chr5_4:22967299-22967318 Msa0844490:intron 20.0%
!! TGAAGTTTAAACAAAATGAA+AGG + chr5_4:22967420-22967439 Msa0844500:intron 20.0%
!! ATAAAGATAAACAATCATAG+AGG + chr5_4:22967453-22967472 Msa0844500:intron 20.0%
!! TAAACCTTTGAATAATTTGA+AGG - chr5_4:22967511-22967530 Msa0844490:intron 20.0%
!! ACAAATATATATTGGAAATC+AGG - chr5_4:22967642-22967661 Msa0844490:five_prime_UTR 20.0%
!! AACTTCAAATCTTCTAATAT+TGG - chr5_4:22968174-22968193 Msa0844490:five_prime_UTR 20.0%
!!! GAAGTAATTAAATTTGTTCT+GGG - chr5_4:22964916-22964935 Msa0844490:five_prime_UTR 20.0%
!!! AGAAGTAATTAAATTTGTTC+TGG - chr5_4:22964917-22964936 Msa0844490:five_prime_UTR 20.0%
!! AAATTTAATTACTTCTTCAC+AGG + chr5_4:22964921-22964940 Msa0844500:CDS 20.0%
! AAATTTGCCAAATGTAGATA+TGG - chr5_4:22965137-22965156 Msa0844490:intron 25.0%
!!! AAGCTTAATAATTGTTTTGG+TGG - chr5_4:22965217-22965236 Msa0844490:intron 25.0%
!! AGGAAGCTTAATAATTGTTT+TGG - chr5_4:22965220-22965239 Msa0844490:intron 25.0%
! TATTGCATGAATAAGAAATC+AGG + chr5_4:22965241-22965260 Msa0844500:intron 25.0%
! GAGACAATTATAACATATTG+GGG + chr5_4:22965442-22965461 Msa0844500:intron 25.0%
! AGACAATTATAACATATTGG+GGG + chr5_4:22965443-22965462 Msa0844500:intron 25.0%
! GTTCTATCATAAACCATATT+TGG + chr5_4:22965600-22965619 Msa0844500:intron 25.0%
! TCTATCATAAACCATATTTG+GGG + chr5_4:22965602-22965621 Msa0844500:intron 25.0%
!! GTTTCTTTAATAGTTTTCCT+AGG + chr5_4:22965659-22965678 Msa0844500:intron 25.0%
!!! AAATTACCACTTAAGTTGAA+AGG - chr5_4:22965978-22965997 Msa0844490:intron 25.0%
! TTACACGGTTTAAATATCTT+GGG - chr5_4:22966103-22966122 Msa0844490:intron 25.0%
! TTTGTTCAATGTAGAAAGAT+AGG - chr5_4:22966563-22966582 Msa0844490:intron 25.0%
!!! ATGAAAGTTTTATAATGCAG+TGG + chr5_4:22966865-22966884 Msa0844500:intron 25.0%
!!! GTGAATCAGTTTTTTTTTCT+AGG + chr5_4:22966887-22966906 Msa0844500:intron 25.0%
!!! GAATCAGTTTTTTTTTCTAG+GGG + chr5_4:22966889-22966908 Msa0844500:intron 25.0%
! GGGTGTGTAAATTAAAAATT+CGG + chr5_4:22967002-22967021 Msa0844500:intron 25.0%
! TAGCTAAATTTGAAGGATAT+TGG + chr5_4:22967256-22967275 Msa0844500:intron 25.0%
! TAAACAATCATAGAGGAAAA+AGG + chr5_4:22967460-22967479 Msa0844500:intron 25.0%
! TGTACCTTCAAATTATTCAA+AGG + chr5_4:22967504-22967523 Msa0844500:intron 25.0%
!!! TGTTTTTCAAATTCCTTCTT+TGG + chr5_4:22967659-22967678 Msa0844500:intron 25.0%
!!! TAAATGAGGTTTTATATCCT+TGG - chr5_4:22967700-22967719 Msa0844490:five_prime_UTR 25.0%
! AATTAAATCATATAAGAGCC+GGG - chr5_4:22967738-22967757 Msa0844490:five_prime_UTR 25.0%
! GAATTAAATCATATAAGAGC+CGG - chr5_4:22967739-22967758 Msa0844490:five_prime_UTR 25.0%
!! AACATATTTGACTTTGAATG+AGG + chr5_4:22967820-22967839 Msa0844500:intron 25.0%
!! GTATTATAAAGAAGTATAGC+AGG - chr5_4:22967988-22968007 Msa0844490:five_prime_UTR 25.0%
! TAGCTCATGCAATAAAAATT+TGG + chr5_4:22968229-22968248 Msa0844500:intron 25.0%
!! TTAAATTTGTTCTGGGATGA+TGG - chr5_4:22964909-22964928 Msa0844490:five_prime_UTR 30.0%
AGACCAAATGAAATGCTATA+CGG + chr5_4:22965180-22965199 Msa0844500:intron 30.0%
ATTCCGTATAGCATTTCATT+TGG - chr5_4:22965186-22965205 Msa0844490:intron 30.0%
ATGTGCATCAAACATATGAT+GGG + chr5_4:22965337-22965356 Msa0844500:intron 30.0%
TTTCAAAAAGAACTTAGGGA+TGG - chr5_4:22965380-22965399 Msa0844490:intron 30.0%
GACAATTATAACATATTGGG+GGG + chr5_4:22965444-22965463 Msa0844500:intron 30.0%
AACAAATTGAACCAAGGTAA+CGG + chr5_4:22965478-22965497 Msa0844500:intron 30.0%
TATGGTTTATGATAGAACAC+TGG - chr5_4:22965598-22965617 Msa0844490:intron 30.0%
AACCATTAGAAAGGATTTAG+AGG - chr5_4:22965642-22965661 Msa0844490:intron 30.0%
GAAAATAGGCTTAGATGATT+TGG - chr5_4:22965770-22965789 Msa0844490:intron 30.0%
! CATCTAAGCCTATTTTCTAT+AGG + chr5_4:22965773-22965792 Msa0844500:intron 30.0%
AACTTTCCTTTCAACTTAAG+TGG + chr5_4:22965969-22965988 Msa0844500:intron 30.0%
TAAATCATCGTATTCAAGCT+AGG - chr5_4:22966043-22966062 Msa0844490:intron 30.0%
GTTACACGGTTTAAATATCT+TGG - chr5_4:22966104-22966123 Msa0844490:intron 30.0%
TATTTAAACCGTGTAACTTG+AGG + chr5_4:22966107-22966126 Msa0844500:intron 30.0%
ATATCATACCTCAAGTTACA+CGG - chr5_4:22966118-22966137 Msa0844490:intron 30.0%
AGTTACATTCCATATGCTTA+AGG + chr5_4:22966186-22966205 Msa0844500:intron 30.0%
! GTTCAATTTTACCCATTTCA+AGG + chr5_4:22966602-22966621 Msa0844500:intron 30.0%
!!! GTTGTTTTGGTATTTTTGGT+CGG - chr5_4:22967105-22967124 Msa0844490:intron 30.0%
!!! GTGTGTTGTTTTGGTATTTT+TGG - chr5_4:22967109-22967128 Msa0844490:intron 30.0%
!!! TTGAATCTTGTGTGTTGTTT+TGG - chr5_4:22967118-22967137 Msa0844490:intron 30.0%
! AACAACTTGGTAGCAAAATT+TGG + chr5_4:22967137-22967156 Msa0844500:intron 30.0%
TAAGAGACAATAGTTACTCA+AGG + chr5_4:22967529-22967548 Msa0844500:intron 30.0%
! ATTTGCTGCAGTAATAAATG+AGG - chr5_4:22967714-22967733 Msa0844490:five_prime_UTR 30.0%
GAAACTACATGAATTTGGAA+TGG - chr5_4:22967761-22967780 Msa0844490:five_prime_UTR 30.0%
TGTAGATCTAAAAACGATGA+TGG + chr5_4:22968025-22968044 Msa0844500:intron 30.0%
!! TGGTATTTTCTTGATGGTTT+GGG - chr5_4:22968064-22968083 Msa0844490:five_prime_UTR 30.0%
AATATCTGATCAAGCAGTTA+TGG - chr5_4:22968562-22968581 Msa0844490:intron 30.0%
AACCTTAAAGATGCAATGTA+GGG + chr5_4:22968591-22968610 Msa0844500:intron 30.0%
!! TTAAATTTGTTCTGGGATGA+TGG - chr5_4:22964909-22964928 Msa0844490:five_prime_UTR 30.0%
GTTTGCTCCATATCTACATT+TGG + chr5_4:22965127-22965146 Msa0844500:intron 35.0%
GATGTGCATCAAACATATGA+TGG + chr5_4:22965336-22965355 Msa0844500:intron 35.0%
AAAAGAACTTAGGGATGGAT+TGG - chr5_4:22965375-22965394 Msa0844490:intron 35.0%
AATCGGAACAAATTGAACCA+AGG + chr5_4:22965472-22965491 Msa0844500:intron 35.0%
GACCTCTAAATCCTTTCTAA+TGG + chr5_4:22965637-22965656 Msa0844500:intron 35.0%
AAGAAGAGTAGATCAAATGG+AGG - chr5_4:22965714-22965733 Msa0844490:intron 35.0%
TGCAAGAAGAGTAGATCAAA+TGG - chr5_4:22965717-22965736 Msa0844490:intron 35.0%
TTGATCTACTCTTCTTGCAA+CGG + chr5_4:22965718-22965737 Msa0844500:intron 35.0%
TGGAATGTAACTTCAGCAAA+AGG - chr5_4:22966178-22966197 Msa0844490:intron 35.0%
ACATTCCATATGCTTAAGGA+TGG + chr5_4:22966190-22966209 Msa0844500:intron 35.0%
! TTTTGCCATCCTTAAGCATA+TGG - chr5_4:22966198-22966217 Msa0844490:intron 35.0%
TATGCTTAAGGATGGCAAAA+CGG + chr5_4:22966198-22966217 Msa0844500:intron 35.0%
!! TGATTTGCTCTGATTCACTT+GGG - chr5_4:22966798-22966817 Msa0844490:intron 35.0%
!!! GTTTTTTTTTCTAGGGGTAG+TGG + chr5_4:22966895-22966914 Msa0844500:intron 35.0%
TCATTTGGCCCAATTGTTTA+TGG + chr5_4:22966979-22966998 Msa0844500:intron 35.0%
CATTTGGCCCAATTGTTTAT+GGG + chr5_4:22966980-22966999 Msa0844500:intron 35.0%
ATTTGGCCCAATTGTTTATG+GGG + chr5_4:22966981-22967000 Msa0844500:intron 35.0%
CACACAAGATTCAAACAACT+TGG + chr5_4:22967124-22967143 Msa0844500:intron 35.0%
GTCAAGATATAACCGTTCAT+TGG + chr5_4:22967200-22967219 Msa0844500:intron 35.0%
! TGATGAAGCATTCCAATGAA+CGG - chr5_4:22967215-22967234 Msa0844490:intron 35.0%
CATTGGAATGCTTCATCAAT+TGG + chr5_4:22967217-22967236 Msa0844500:intron 35.0%
TAATAGACTATCCCGTTCTA+AGG + chr5_4:22967310-22967329 Msa0844500:intron 35.0%
TCATTGATGTGACCTTAGAA+CGG - chr5_4:22967325-22967344 Msa0844490:intron 35.0%
ACAATCATAGAGGAAAAAGG+AGG + chr5_4:22967463-22967482 Msa0844500:intron 35.0%
! TAGCTGTTTGAAACAAACAC+AGG + chr5_4:22967584-22967603 Msa0844500:intron 35.0%
TTATTACTGCAGCAAATTCC+CGG + chr5_4:22967717-22967736 Msa0844500:intron 35.0%
ACTGACATCATAAGTTTCTG+TGG + chr5_4:22967928-22967947 Msa0844500:intron 35.0%
ATCATAAGTTTCTGTGGAAG+AGG + chr5_4:22967934-22967953 Msa0844500:intron 35.0%
CAGGAAATGTGATAAACTCT+AGG - chr5_4:22967969-22967988 Msa0844490:five_prime_UTR 35.0%
!! GGTATTTTCTTGATGGTTTG+GGG - chr5_4:22968063-22968082 Msa0844490:five_prime_UTR 35.0%
!! CTGGTATTTTCTTGATGGTT+TGG - chr5_4:22968065-22968084 Msa0844490:five_prime_UTR 35.0%
TGGAAGATCTAAATGCTGAT+GGG - chr5_4:22968154-22968173 Msa0844490:five_prime_UTR 35.0%
TTGGAAGATCTAAATGCTGA+TGG - chr5_4:22968155-22968174 Msa0844490:five_prime_UTR 35.0%
TTGATACAAAGCCCTTAACA+TGG + chr5_4:22968337-22968356 Msa0844500:intron 35.0%
TTATGTAGCGACCATGTTAA+GGG - chr5_4:22968351-22968370 Msa0844490:intron 35.0%
TTTATGTAGCGACCATGTTA+AGG - chr5_4:22968352-22968371 Msa0844490:intron 35.0%
! ATGGTGTTTCTGATTCAGTA+TGG - chr5_4:22968543-22968562 Msa0844490:intron 35.0%
CAACCTTAAAGATGCAATGT+AGG + chr5_4:22968590-22968609 Msa0844500:intron 35.0%
TTCCCTACATTGCATCTTTA+AGG - chr5_4:22968596-22968615 Msa0844490:five_prime_UTR 35.0%
TAAAGATGCAATGTAGGGAA+TGG + chr5_4:22968596-22968615 Msa0844500:intron 35.0%
ATCTTAGTGAAATTGCACTC+CGG + chr5_4:22968740-22968759 Msa0844500:CDS 35.0%
!!! CTGGAGTTTTGGTTGTACAT+CGG - chr5_4:22964859-22964878 Msa0844490:five_prime_UTR 40.0%
AAAACTCCAGGCATCTGATT+CGG + chr5_4:22964869-22964888 Msa0844500:CDS 40.0%
ATCTTAGTGGAATTGCACTC+CGG + chr5_4:22964959-22964978 Msa0844500:CDS 40.0%
!! TTCTCGTTTGATGGATTGAC+CGG - chr5_4:22964981-22965000 Msa0844490:five_prime_UTR 40.0%
AGGTCCATTATCAGAAACCA+CGG + chr5_4:22965021-22965040 Msa0844500:intron 40.0%
CATATTGGGGGGACATAAAT+CGG + chr5_4:22965455-22965474 Msa0844500:intron 40.0%
ATATGGCATATCCGTTACCT+TGG - chr5_4:22965492-22965511 Msa0844490:intron 40.0%
! CAAGTCTTATCCCACTAAGT+GGG + chr5_4:22965548-22965567 Msa0844500:intron 40.0%
! AAGTCTTATCCCACTAAGTG+GGG + chr5_4:22965549-22965568 Msa0844500:intron 40.0%
! ATGAGTTGGATCCCCAAATA+TGG - chr5_4:22965616-22965635 Msa0844490:intron 40.0%
GGATTTAGAGGTCAATGAGT+TGG - chr5_4:22965630-22965649 Msa0844490:intron 40.0%
AATCAGGTTCAAAGAGGTAG+AGG - chr5_4:22965689-22965708 Msa0844490:intron 40.0%
AGATCAAATGGAGGAGAATC+AGG - chr5_4:22965705-22965724 Msa0844490:intron 40.0%
GGGTAGCACCTATAGAAAAT+AGG - chr5_4:22965784-22965803 Msa0844490:intron 40.0%
AATGACACCATTAGAGAGAG+CGG - chr5_4:22965807-22965826 Msa0844490:intron 40.0%
CGAGAGTTAAGTAAGTGTAG+CGG - chr5_4:22965883-22965902 Msa0844490:intron 40.0%
CACTTACTTAACTCTCGTGT+TGG + chr5_4:22965886-22965905 Msa0844500:intron 40.0%
ACTGACGATGTTACATGGTA+CGG - chr5_4:22965931-22965950 Msa0844490:intron 40.0%
CTTATTGCACAAATCACCTG+AGG + chr5_4:22965998-22966017 Msa0844500:intron 40.0%
TTCAAGCTAGGTGGTTGAAA+TGG - chr5_4:22966031-22966050 Msa0844490:intron 40.0%
ATCATCGTATTCAAGCTAGG+TGG - chr5_4:22966040-22966059 Msa0844490:intron 40.0%
GGGTGCATAGTACAAAATGA+CGG - chr5_4:22966083-22966102 Msa0844490:intron 40.0%
ACTTCAGCAAAAGGTGAAGT+AGG - chr5_4:22966169-22966188 Msa0844490:intron 40.0%
ATGCTTAAGGATGGCAAAAC+GGG + chr5_4:22966199-22966218 Msa0844500:intron 40.0%
CTCTGATTCACTTGGGTTTA+AGG - chr5_4:22966791-22966810 Msa0844490:intron 40.0%
!! CTGATTTGCTCTGATTCACT+TGG - chr5_4:22966799-22966818 Msa0844490:intron 40.0%
!!! AGAGGATGAGTTTTGAGTCT+AGG + chr5_4:22966842-22966861 Msa0844500:intron 40.0%
AGTTATCGCTTATCAGAGAG+AGG + chr5_4:22966932-22966951 Msa0844500:intron 40.0%
GTTATCGCTTATCAGAGAGA+GGG + chr5_4:22966933-22966952 Msa0844500:intron 40.0%
GGACAAATTGAGGTTGTGTT+GGG + chr5_4:22966954-22966973 Msa0844500:intron 40.0%
TTTGGCCCAATTGTTTATGG+GGG + chr5_4:22966982-22967001 Msa0844500:intron 40.0%
ACACACCCCCATAAACAATT+GGG - chr5_4:22966990-22967009 Msa0844490:intron 40.0%
TACACACCCCCATAAACAAT+TGG - chr5_4:22966991-22967010 Msa0844490:intron 40.0%
ATATCTTGACACTGGAGTAC+AGG - chr5_4:22967190-22967209 Msa0844490:intron 40.0%
GAACGGTTATATCTTGACAC+TGG - chr5_4:22967198-22967217 Msa0844490:intron 40.0%
CATTGATGTGACCTTAGAAC+GGG - chr5_4:22967324-22967343 Msa0844490:intron 40.0%
TACTCATGCATCAACACATC+TGG + chr5_4:22967353-22967372 Msa0844500:intron 40.0%
!! GTGAGGAGTGTTAAATCAAG+TGG + chr5_4:22967375-22967394 Msa0844500:intron 40.0%
CGTGGGAAACTACATGAATT+TGG - chr5_4:22967766-22967785 Msa0844490:five_prime_UTR 40.0%
! AGGTTGTTGGTTATACTCGT+GGG - chr5_4:22967783-22967802 Msa0844490:five_prime_UTR 40.0%
GAGTATAACCAACAACCTGT+GGG + chr5_4:22967785-22967804 Msa0844500:intron 40.0%
GTTTAACAGCTTATACCCAC+AGG - chr5_4:22967803-22967822 Msa0844490:five_prime_UTR 40.0%
! GGTGACTGGTATTTTCTTGA+TGG - chr5_4:22968070-22968089 Msa0844490:five_prime_UTR 40.0%
! GATGACTTGCAGTTGCATTT+GGG - chr5_4:22968091-22968110 Msa0844490:five_prime_UTR 40.0%
! TGATGACTTGCAGTTGCATT+TGG - chr5_4:22968092-22968111 Msa0844490:five_prime_UTR 40.0%
! TCTAAATGCTGATGGGCAAA+CGG - chr5_4:22968147-22968166 Msa0844490:five_prime_UTR 40.0%
TTCCGGTAGACCAAATATGA+CGG - chr5_4:22968281-22968300 Msa0844490:five_prime_UTR 40.0%
CTTTGTATCAAATGTGCTGG+AGG - chr5_4:22968328-22968347 Msa0844490:five_prime_UTR 40.0%
!!! CTGGAGTTTTGGTTGTACAT+CGG - chr5_4:22964859-22964878 Msa0844490:five_prime_UTR 40.0%
AAAACTCCAGGCATCTGATT+CGG + chr5_4:22964869-22964888 Msa0844500:CDS 40.0%
GCAATTTCACTAAGATCCAG+AGG - chr5_4:22968736-22968755 Msa0844490:five_prime_UTR 40.0%
!! TTCTCGTTTGATGGATTGAC+CGG - chr5_4:22964981-22965000 Msa0844490:five_prime_UTR 40.0%
GAAGAAACCTTCTCGTTTGA+TGG - chr5_4:22968771-22968790 Msa0844490:five_prime_UTR 40.0%
GGTTTCTTCAGAATTGTAGC+AGG + chr5_4:22968782-22968801 Msa0844500:CDS 40.0%
AGGTCCATTATCAGAAACCA+CGG + chr5_4:22965021-22965040 Msa0844500:intron 40.0%
CGATGTACAACCAAAACTCC+AGG + chr5_4:22964857-22964876 Msa0844500:CDS 45.0%
! GAATCAGATGCCTGGAGTTT+TGG - chr5_4:22964870-22964889 Msa0844490:five_prime_UTR 45.0%
AAACTCCAGGCATCTGATTC+GGG + chr5_4:22964870-22964889 Msa0844500:CDS 45.0%
TTACTTCTTCACAGGTTCCC+AGG + chr5_4:22964929-22964948 Msa0844500:CDS 45.0%
GCAATTCCACTAAGATCCAG+AGG - chr5_4:22964955-22964974 Msa0844490:five_prime_UTR 45.0%
GGTCAATCCATCAAACGAGA+AGG + chr5_4:22964980-22964999 Msa0844500:CDS 45.0%
GAAGAAGCCTTCTCGTTTGA+TGG - chr5_4:22964990-22965009 Msa0844490:five_prime_UTR 45.0%
GGCTTCTTCAGAATTGTAGC+AGG + chr5_4:22965001-22965020 Msa0844500:CDS 45.0%
CATGCCGTGGTTTCTGATAA+TGG - chr5_4:22965028-22965047 Msa0844490:five_prime_UTR 45.0%
ATGGAGCAAACATCGCATGA+GGG - chr5_4:22965118-22965137 Msa0844490:five_prime_UTR 45.0%
TATGGAGCAAACATCGCATG+AGG - chr5_4:22965119-22965138 Msa0844490:five_prime_UTR 45.0%
!! GAACTTAGGGATGGATTGGT+GGG - chr5_4:22965371-22965390 Msa0844490:intron 45.0%
!! AGAACTTAGGGATGGATTGG+TGG - chr5_4:22965372-22965391 Msa0844490:intron 45.0%
CCACTTAGTGGGATAAGACT+TGG - chr5_4:22965550-22965569 Msa0844490:intron 45.0%
! CCAAGTCTTATCCCACTAAG+TGG + chr5_4:22965547-22965566 Msa0844500:intron 45.0%
AAAGAGGTAGAGGAAGACCT+AGG - chr5_4:22965679-22965698 Msa0844490:intron 45.0%
GAGGAGAATCAGGTTCAAAG+AGG - chr5_4:22965695-22965714 Msa0844490:intron 45.0%
TCTTCTTGCAACGGCATCTA+CGG + chr5_4:22965727-22965746 Msa0844500:intron 45.0%
ATGACACCATTAGAGAGAGC+GGG - chr5_4:22965806-22965825 Msa0844490:intron 45.0%
! AAGTAAGTGTAGCGGAGATG+AGG - chr5_4:22965875-22965894 Msa0844490:intron 45.0%
! GTTACATGGTACGGAGTGTT+GGG - chr5_4:22965922-22965941 Msa0844490:intron 45.0%
TGTTACATGGTACGGAGTGT+TGG - chr5_4:22965923-22965942 Msa0844490:intron 45.0%
CTCAGACTGACGATGTTACA+TGG - chr5_4:22965936-22965955 Msa0844490:intron 45.0%
TTGAAATGGAGAAGAGCCTC+AGG - chr5_4:22966017-22966036 Msa0844490:intron 45.0%
ATCTCCAACTTTCACCTCCA+AGG + chr5_4:22966139-22966158 Msa0844500:intron 45.0%
TCTACCTTGGAGGTGAAAGT+TGG - chr5_4:22966146-22966165 Msa0844490:intron 45.0%
AAGGTGAAGTAGGTCTACCT+TGG - chr5_4:22966159-22966178 Msa0844490:intron 45.0%
!!! GATTTTGACGGGCTAATAGC+GGG - chr5_4:22966234-22966253 Msa0844490:intron 45.0%
!!! CGATTTTGACGGGCTAATAG+CGG - chr5_4:22966235-22966254 Msa0844490:intron 45.0%
CGCTATTAGCCCGTCAAAAT+CGG + chr5_4:22966233-22966252 Msa0844500:intron 45.0%
GCTATTAGCCCGTCAAAATC+GGG + chr5_4:22966234-22966253 Msa0844500:intron 45.0%
CTATTAGCCCGTCAAAATCG+GGG + chr5_4:22966235-22966254 Msa0844500:intron 45.0%
TCTCTCTTTCGCTCTACTCT+CGG - chr5_4:22966823-22966842 Msa0844490:intron 45.0%
TCAGAGAGAGGGACAAATTG+AGG + chr5_4:22966944-22966963 Msa0844500:intron 45.0%
GGGACAAATTGAGGTTGTGT+TGG + chr5_4:22966953-22966972 Msa0844500:intron 45.0%
!! AGGTTGTGTTGGGTGTCATT+TGG + chr5_4:22966964-22966983 Msa0844500:intron 45.0%
ATGCATCAACACATCTGGTG+AGG + chr5_4:22967358-22967377 Msa0844500:intron 45.0%
CAATGCAAGACAGCCAAAGA+AGG - chr5_4:22967675-22967694 Msa0844490:five_prime_UTR 45.0%
! CAGGTTGTTGGTTATACTCG+TGG - chr5_4:22967784-22967803 Msa0844490:five_prime_UTR 45.0%
CGAGTATAACCAACAACCTG+TGG + chr5_4:22967784-22967803 Msa0844500:intron 45.0%
AGCTTATACCCACAGGTTGT+TGG - chr5_4:22967796-22967815 Msa0844490:five_prime_UTR 45.0%
! GTTCAGTGTGGCGAATTTTC+TGG - chr5_4:22967886-22967905 Msa0844490:five_prime_UTR 45.0%
ACCAAGAAAGCAGTTCAGTG+TGG - chr5_4:22967898-22967917 Msa0844490:five_prime_UTR 45.0%
ACACTGAACTGCTTTCTTGG+TGG + chr5_4:22967897-22967916 Msa0844500:intron 45.0%
! TTCATTTTCCGATGCAGCCT+TGG + chr5_4:22968197-22968216 Msa0844500:intron 45.0%
!! ATGAGCTAGCTTTCTTGCCA+AGG - chr5_4:22968217-22968236 Msa0844490:five_prime_UTR 45.0%
! AAATATGACGGGGATGTCAC+TGG - chr5_4:22968269-22968288 Msa0844490:five_prime_UTR 45.0%
AGTGACATCCCCGTCATATT+TGG + chr5_4:22968268-22968287 Msa0844500:intron 45.0%
TCCGGTAGACCAAATATGAC+GGG - chr5_4:22968280-22968299 Msa0844490:five_prime_UTR 45.0%
AGTACCTGAATCAGAGCTTC+CGG - chr5_4:22968298-22968317 Msa0844490:five_prime_UTR 45.0%
! GAAGCTCTGATTCAGGTACT+GGG + chr5_4:22968298-22968317 Msa0844500:intron 45.0%
! AAGCTCTGATTCAGGTACTG+GGG + chr5_4:22968299-22968318 Msa0844500:intron 45.0%
! GGGCTTTGTATCAAATGTGC+TGG - chr5_4:22968331-22968350 Msa0844490:five_prime_UTR 45.0%
CGATGTACAACCAAAACTCC+AGG + chr5_4:22964857-22964876 Msa0844500:CDS 45.0%
! GAATCAGATGCCTGGAGTTT+TGG - chr5_4:22964870-22964889 Msa0844490:five_prime_UTR 45.0%
AAACTCCAGGCATCTGATTC+GGG + chr5_4:22964870-22964889 Msa0844500:CDS 45.0%
TTACTTCTTCACAGGTTCCC+AGG + chr5_4:22964929-22964948 Msa0844500:CDS 45.0%
GGTCAATCCATCAAACGAGA+AGG + chr5_4:22964980-22964999 Msa0844500:CDS 45.0%
CATGCCGTGGTTTCTGATAA+TGG - chr5_4:22965028-22965047 Msa0844490:five_prime_UTR 45.0%
!!! AAATATTTTAAAAAATAAAC+GGG - chr5_4:22966334-22966353 Msa0844490:intron 5.0%
TATCTCCCGAATCAGATGCC+TGG - chr5_4:22964878-22964897 Msa0844490:five_prime_UTR 50.0%
GACCATTCGCATCAAGAAGC+AGG + chr5_4:22965047-22965066 Msa0844500:intron 50.0%
AGCCTGCTTCTTGATGCGAA+TGG - chr5_4:22965052-22965071 Msa0844490:five_prime_UTR 50.0%
GTTGTTGAGCTTCACACTGC+AGG - chr5_4:22965095-22965114 Msa0844490:five_prime_UTR 50.0%
TGGAGCAAACATCGCATGAG+GGG - chr5_4:22965117-22965136 Msa0844490:five_prime_UTR 50.0%
CTTATCCCACTAAGTGGGGT+CGG + chr5_4:22965553-22965572 Msa0844500:intron 50.0%
CTTCTTGCAACGGCATCTAC+GGG + chr5_4:22965728-22965747 Msa0844500:intron 50.0%
TGACACCATTAGAGAGAGCG+GGG - chr5_4:22965805-22965824 Msa0844490:intron 50.0%
AGGATGTTGCGTTGGATGAG+TGG - chr5_4:22965855-22965874 Msa0844490:intron 50.0%
GTGAAGTAGGTCTACCTTGG+AGG - chr5_4:22966156-22966175 Msa0844490:intron 50.0%
GAGTAGAGCGAAAGAGAGAG+AGG + chr5_4:22966824-22966843 Msa0844500:intron 50.0%
! TTCTAGGGGTAGTGGTCTAG+TGG + chr5_4:22966903-22966922 Msa0844500:intron 50.0%
! GGCTGTCTTGCATTGATCCA+AGG + chr5_4:22967680-22967699 Msa0844500:intron 50.0%
GCCACACTGAACTGCTTTCT+TGG + chr5_4:22967894-22967913 Msa0844500:intron 50.0%
! ATGGTTTGGGGCTCGAAAGA+GGG - chr5_4:22968051-22968070 Msa0844490:five_prime_UTR 50.0%
TGCAGTTGCATTTGGGTGAC+TGG - chr5_4:22968084-22968103 Msa0844490:five_prime_UTR 50.0%
CTTTCTTGCCAAGGCTGCAT+CGG - chr5_4:22968208-22968227 Msa0844490:five_prime_UTR 50.0%
! GGATGTCACTGGTGTTAGGT+TGG - chr5_4:22968258-22968277 Msa0844490:five_prime_UTR 50.0%
CCGGTAGACCAAATATGACG+GGG - chr5_4:22968279-22968298 Msa0844490:five_prime_UTR 50.0%
CCCCGTCATATTTGGTCTAC+CGG + chr5_4:22968276-22968295 Msa0844500:intron 50.0%
! TCTACCGGAAGCTCTGATTC+AGG + chr5_4:22968291-22968310 Msa0844500:intron 50.0%
! GGAAGCTCTGATTCAGGTAC+TGG + chr5_4:22968297-22968316 Msa0844500:intron 50.0%
TATCTCCCGAATCAGATGCC+TGG - chr5_4:22964878-22964897 Msa0844490:five_prime_UTR 50.0%
TCACTAAGATCCAGAGGCCT+GGG - chr5_4:22968730-22968749 Msa0844490:five_prime_UTR 50.0%
TTCACTAAGATCCAGAGGCC+TGG - chr5_4:22968731-22968750 Msa0844490:five_prime_UTR 50.0%
CCACTAAGATCCAGAGGCCT+GGG - chr5_4:22964949-22964968 Msa0844490:five_prime_UTR 55.0%
TCCACTAAGATCCAGAGGCC+TGG - chr5_4:22964950-22964969 Msa0844490:five_prime_UTR 55.0%
TGATGCGAATGGTCATGCCG+TGG - chr5_4:22965041-22965060 Msa0844490:five_prime_UTR 55.0%
TGTAGCCGACCCCACTTAGT+GGG - chr5_4:22965561-22965580 Msa0844490:intron 55.0%
ATGTAGCCGACCCCACTTAG+TGG - chr5_4:22965562-22965581 Msa0844490:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr5_4:22965561-22965580 Msa0844500:intron 55.0%
GACACCATTAGAGAGAGCGG+GGG - chr5_4:22965804-22965823 Msa0844490:intron 55.0%
CGGAGATGAGGATGTTGCGT+TGG - chr5_4:22965863-22965882 Msa0844490:intron 55.0%
!! ACATGGTACGGAGTGTTGGG+CGG - chr5_4:22965919-22965938 Msa0844490:intron 55.0%
TTAGCCCGTCAAAATCGGGG+TGG + chr5_4:22966238-22966257 Msa0844500:intron 55.0%
TAGCCCGTCAAAATCGGGGT+GGG + chr5_4:22966239-22966258 Msa0844500:intron 55.0%
!! AGTCCCACCCCGATTTTGAC+GGG - chr5_4:22966245-22966264 Msa0844490:intron 55.0%
! CAGTCCCACCCCGATTTTGA+CGG - chr5_4:22966246-22966265 Msa0844490:intron 55.0%
GTCAAAATCGGGGTGGGACT+GGG + chr5_4:22966245-22966264 Msa0844500:intron 55.0%
TTGAGCCCATCCCCTCAAGT+TGG - chr5_4:22966275-22966294 Msa0844490:intron 55.0%
CAAACTCACACCCCGCCTAA+AGG + chr5_4:22966292-22966311 Msa0844500:intron 55.0%
! GATGGTTTGGGGCTCGAAAG+AGG - chr5_4:22968052-22968071 Msa0844490:five_prime_UTR 55.0%
ACGGGGATGTCACTGGTGTT+AGG - chr5_4:22968262-22968281 Msa0844490:five_prime_UTR 55.0%
TTCACAGGTTCCCAGGCCTC+TGG + chr5_4:22964936-22964955 Msa0844500:CDS 60.0%
CCCAGGCCTCTGGATCTTAG+TGG + chr5_4:22964946-22964965 Msa0844500:CDS 60.0%
GCTACCCCCGCTCTCTCTAA+TGG + chr5_4:22965797-22965816 Msa0844500:intron 60.0%
!! TGACGGGCTAATAGCGGGCT+GGG - chr5_4:22966229-22966248 Msa0844490:intron 60.0%
!! TTGACGGGCTAATAGCGGGC+TGG - chr5_4:22966230-22966249 Msa0844490:intron 60.0%
CGTCAAAATCGGGGTGGGAC+TGG + chr5_4:22966244-22966263 Msa0844500:intron 60.0%
AAAATCGGGGTGGGACTGGG+CGG + chr5_4:22966248-22966267 Msa0844500:intron 60.0%
ACTCACACCCCGCCTAAAGG+CGG + chr5_4:22966295-22966314 Msa0844500:intron 60.0%
TAGCCCGCTAGTCCGCCTTT+AGG - chr5_4:22966310-22966329 Msa0844490:intron 60.0%
ATACCAGTCCCCAAGCGACG+TGG + chr5_4:22968117-22968136 Msa0844500:intron 60.0%
AACGGCTCTCCACGTCGCTT+GGG - chr5_4:22968129-22968148 Msa0844490:five_prime_UTR 60.0%
AAACGGCTCTCCACGTCGCT+TGG - chr5_4:22968130-22968149 Msa0844490:five_prime_UTR 60.0%
TTCACAGGTTCCCAGGCCTC+TGG + chr5_4:22964936-22964955 Msa0844500:CDS 60.0%
AAATCGGGGTGGGACTGGGC+GGG + chr5_4:22966249-22966268 Msa0844500:intron 65.0%
GACTGGGCGGGCCAACTTGA+GGG + chr5_4:22966261-22966280 Msa0844500:intron 65.0%
ACTGGGCGGGCCAACTTGAG+GGG + chr5_4:22966262-22966281 Msa0844500:intron 65.0%
GCGGGCCAACTTGAGGGGAT+GGG + chr5_4:22966267-22966286 Msa0844500:intron 65.0%
CGCTAGTCCGCCTTTAGGCG+GGG - chr5_4:22966305-22966324 Msa0844490:intron 65.0%
CCGCTAGTCCGCCTTTAGGC+GGG - chr5_4:22966306-22966325 Msa0844490:intron 65.0%
!! CCCGCCTAAAGGCGGACTAG+CGG + chr5_4:22966303-22966322 Msa0844500:intron 65.0%
CCCGCTAGTCCGCCTTTAGG+CGG - chr5_4:22966307-22966326 Msa0844490:intron 65.0%
!! CCGCCTAAAGGCGGACTAGC+GGG + chr5_4:22966304-22966323 Msa0844500:intron 65.0%
TCTCCACGTCGCTTGGGGAC+TGG - chr5_4:22968123-22968142 Msa0844490:five_prime_UTR 65.0%
ACGGCTCTCCACGTCGCTTG+GGG - chr5_4:22968128-22968147 Msa0844490:five_prime_UTR 65.0%
!! GGGCTAATAGCGGGCTGGGC+TGG - chr5_4:22966225-22966244 Msa0844490:intron 70.0%
GGACTGGGCGGGCCAACTTG+AGG + chr5_4:22966260-22966279 Msa0844500:intron 70.0%
GGCGGGCCAACTTGAGGGGA+TGG + chr5_4:22966266-22966285 Msa0844500:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 22964820 22968829 22964820 ID=Msa0844500;Name=Msa0844500
chr5_4 mRNA 22964820 22968829 22964820 ID=Msa0844500-mRNA-1;Parent=Msa0844500;Name=Msa0844500-mRNA-1;_AED=0.18;_eAED=0.77;_QI=0|0|0|1|0|0|2|0|102
chr5_4 exon 22964820 22965022 22964820 ID=Msa0844500-mRNA-1:exon:9370;Parent=Msa0844500-mRNA-1
chr5_4 exon 22968724 22968829 22968724 ID=Msa0844500-mRNA-1:exon:9371;Parent=Msa0844500-mRNA-1
chr5_4 CDS 22964820 22965022 22964820 ID=Msa0844500-mRNA-1:cds;Parent=Msa0844500-mRNA-1
chr5_4 CDS 22968724 22968829 22968724 ID=Msa0844500-mRNA-1:cds;Parent=Msa0844500-mRNA-1
Gene Sequence

>Msa0844500

ATGCAATGTAGAGAATGGAGCGCGTGCACTTCTCAACCGATGTACAACCAAAACTCCAGGCATCTGATTCGGGAGATATATCTATTCCATCATCCCAGAACAAATTTAATTACTTCTTCACAGGTTCCCAGGCCTCTGGATCTTAGTGGAATTGCACTCCGGTCAATCCATCAAACGAGAAGGCTTCTTCAGAATTGTAGCAGGTTCCCAGGCCTCTGGATCTTAGTGAAATTGCACTCCGGTCAATCCATCAAACGAGAAGGTTTCTTCAGAATTGTAGCAGGTCCATTATCAGAAACCACGGCATGA
Protein sequence

>Msa0844500

MQCREWSACTSQPMYNQNSRHLIREIYLFHHPRTNLITSSQVPRPLDLSGIALRSIHQTRRLLQNCSRFPGLWILVKLHSGQSIKREGFFRIVAGPLSETTA*