AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1060840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1060840 MtrunA17_Chr1g0172351 77.143 70 16 0 99 168 77 146 1.98e-35 122
Msa1060840 MtrunA17_Chr4g0044671 25.229 218 118 2 1 175 1 216 1.51e-21 87.8
Msa1060840 MtrunA17_Chr4g0034781 25.472 212 113 2 7 175 1 210 3.86e-20 84.0
Msa1060840 MtrunA17_Chr1g0175581 50.000 78 39 0 88 165 29 106 8.36e-20 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGGTTGGACTGAGTTTA+AGG 0.185372 7_2:+49533070 Msa1060840:CDS
GGAAAACTTACAACACAATT+TGG 0.198179 7_2:+49533491 Msa1060840:CDS
CAGCAAGTTGATGACGAATT+TGG 0.199887 7_2:+49533377 Msa1060840:intron
TGGCAATGAAGAGAAATTTA+AGG 0.284538 7_2:+49533451 Msa1060840:CDS
GGAATGTATCTCAAGCAATA+TGG 0.328623 7_2:+49533398 Msa1060840:CDS
ATAAAACTTTGTGCTGATAA+TGG 0.338821 7_2:+49533431 Msa1060840:CDS
TAGCTAATCTATCACGTTCA+TGG 0.343597 7_2:+49532976 Msa1060840:CDS
TCAATTTGAAGCGTATTGTT+TGG 0.372308 7_2:-49533559 None:intergenic
AAGGTGTATTTACTTAATGA+TGG 0.416212 7_2:+49533470 Msa1060840:CDS
AGTTGTCAAGGTTGCAGAGC+AGG 0.420128 7_2:+49532800 Msa1060840:CDS
TCTCAAGCAATATGGTTTCC+AGG 0.423438 7_2:+49533406 Msa1060840:CDS
TTGGCCAACTCTGAAGTTGT+TGG 0.427754 7_2:-49533540 None:intergenic
AAAACAATTTCAACATTGTC+AGG 0.431374 7_2:-49533107 None:intergenic
AAATTTGTCTTCACAAAAGC+TGG 0.439036 7_2:-49532954 None:intergenic
CTACGGTAATAATATGTTCT+CGG 0.473564 7_2:+49533136 Msa1060840:CDS
TTTAACTTTCAACAAAAGCT+TGG 0.486750 7_2:+49533031 Msa1060840:CDS
GAAGCAATGGAAGATGAAGC+AGG 0.524686 7_2:-49533625 None:intergenic
TCCATTGCTTCTGCTTAGTG+AGG 0.525648 7_2:+49533637 Msa1060840:CDS
TGAATATTTCTCAGTTGTCA+AGG 0.527274 7_2:+49532788 Msa1060840:CDS
CAACACAATTTGGAATCCTG+TGG 0.534831 7_2:+49533501 Msa1060840:CDS
ACCCCATGTTAACACATGGT+TGG 0.537028 7_2:+49533057 Msa1060840:CDS
AGTCCAACCATGTGTTAACA+TGG 0.542552 7_2:-49533060 None:intergenic
AGCAATGGAAGATGAAGCAG+GGG 0.564179 7_2:-49533623 None:intergenic
AACACAATTTGGAATCCTGT+GGG 0.587367 7_2:+49533502 Msa1060840:CDS
ACCTCACTAAGCAGAAGCAA+TGG 0.589982 7_2:-49533638 None:intergenic
GTCCAACCATGTGTTAACAT+GGG 0.594446 7_2:-49533059 None:intergenic
TCTTGCAGAAATGATCCCAC+AGG 0.594556 7_2:-49533517 None:intergenic
AAGACCAACAACTTCAGAGT+TGG 0.597015 7_2:+49533536 Msa1060840:CDS
AAGCAATGGAAGATGAAGCA+GGG 0.606092 7_2:-49533624 None:intergenic
GTTAACCCCATGTTAACACA+TGG 0.616537 7_2:+49533053 Msa1060840:CDS
CAACTGAGAAATATTCAAGC+GGG 0.617358 7_2:-49532782 None:intergenic
TCCAACCATGTGTTAACATG+GGG 0.650672 7_2:-49533058 None:intergenic
ACAACTGAGAAATATTCAAG+CGG 0.670299 7_2:-49532783 None:intergenic
CTTCTCAATACAGAAAACAC+AGG 0.686274 7_2:+49533002 Msa1060840:CDS
GCAATGGAAGATGAAGCAGG+GGG 0.758923 7_2:-49533622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAAAAATCTTCATT+GGG - chr7_2:49533280-49533299 None:intergenic 10.0%
!! ATAAAATAAAAAATCTTCAT+TGG - chr7_2:49533281-49533300 None:intergenic 10.0%
!! TAAAATAAAAAATCTTCATT+GGG - chr7_2:49533280-49533299 None:intergenic 10.0%
!! ATAAAATAAAAAATCTTCAT+TGG - chr7_2:49533281-49533300 None:intergenic 10.0%
!!! ATTTTAAAAGAAGTGAAAAA+AGG - chr7_2:49533307-49533326 None:intergenic 15.0%
!!! ATTTTAAAAGAAGTGAAAAA+AGG - chr7_2:49533307-49533326 None:intergenic 15.0%
!! TTTAACTTTCAACAAAAGCT+TGG + chr7_2:49533031-49533050 Msa1060840:CDS 25.0%
! AAAACAATTTCAACATTGTC+AGG - chr7_2:49533110-49533129 None:intergenic 25.0%
!! GACAATGTTGAAATTGTTTT+TGG + chr7_2:49533110-49533129 Msa1060840:CDS 25.0%
!!! GAAATTGTTTTTGGTTACTA+CGG + chr7_2:49533119-49533138 Msa1060840:CDS 25.0%
! AATGTTAAATGAAGAAGGAA+TGG - chr7_2:49533334-49533353 None:intergenic 25.0%
! GGATAAATGTTAAATGAAGA+AGG - chr7_2:49533339-49533358 None:intergenic 25.0%
! TCATTTAACATTTATCCGAT+TGG + chr7_2:49533342-49533361 Msa1060840:intron 25.0%
! CTGTATAAAACAATACCAAT+CGG - chr7_2:49533360-49533379 None:intergenic 25.0%
!! ATAAAACTTTGTGCTGATAA+TGG + chr7_2:49533431-49533450 Msa1060840:CDS 25.0%
! AAGGTGTATTTACTTAATGA+TGG + chr7_2:49533470-49533489 Msa1060840:CDS 25.0%
!! TTTAACTTTCAACAAAAGCT+TGG + chr7_2:49533031-49533050 Msa1060840:CDS 25.0%
! AAAACAATTTCAACATTGTC+AGG - chr7_2:49533110-49533129 None:intergenic 25.0%
!! GACAATGTTGAAATTGTTTT+TGG + chr7_2:49533110-49533129 Msa1060840:CDS 25.0%
!!! GAAATTGTTTTTGGTTACTA+CGG + chr7_2:49533119-49533138 Msa1060840:CDS 25.0%
! AATGTTAAATGAAGAAGGAA+TGG - chr7_2:49533334-49533353 None:intergenic 25.0%
! GGATAAATGTTAAATGAAGA+AGG - chr7_2:49533339-49533358 None:intergenic 25.0%
! TCATTTAACATTTATCCGAT+TGG + chr7_2:49533342-49533361 Msa1060840:intron 25.0%
! CTGTATAAAACAATACCAAT+CGG - chr7_2:49533360-49533379 None:intergenic 25.0%
!! ATAAAACTTTGTGCTGATAA+TGG + chr7_2:49533431-49533450 Msa1060840:CDS 25.0%
! AAGGTGTATTTACTTAATGA+TGG + chr7_2:49533470-49533489 Msa1060840:CDS 25.0%
ACAACTGAGAAATATTCAAG+CGG - chr7_2:49532786-49532805 None:intergenic 30.0%
TGAATATTTCTCAGTTGTCA+AGG + chr7_2:49532788-49532807 Msa1060840:CDS 30.0%
AAATTTGTCTTCACAAAAGC+TGG - chr7_2:49532957-49532976 None:intergenic 30.0%
CTACGGTAATAATATGTTCT+CGG + chr7_2:49533136-49533155 Msa1060840:CDS 30.0%
TGGCAATGAAGAGAAATTTA+AGG + chr7_2:49533451-49533470 Msa1060840:CDS 30.0%
GGAAAACTTACAACACAATT+TGG + chr7_2:49533491-49533510 Msa1060840:CDS 30.0%
! TCAATTTGAAGCGTATTGTT+TGG - chr7_2:49533562-49533581 None:intergenic 30.0%
ACAACTGAGAAATATTCAAG+CGG - chr7_2:49532786-49532805 None:intergenic 30.0%
TGAATATTTCTCAGTTGTCA+AGG + chr7_2:49532788-49532807 Msa1060840:CDS 30.0%
AAATTTGTCTTCACAAAAGC+TGG - chr7_2:49532957-49532976 None:intergenic 30.0%
CTACGGTAATAATATGTTCT+CGG + chr7_2:49533136-49533155 Msa1060840:CDS 30.0%
TGGCAATGAAGAGAAATTTA+AGG + chr7_2:49533451-49533470 Msa1060840:CDS 30.0%
GGAAAACTTACAACACAATT+TGG + chr7_2:49533491-49533510 Msa1060840:CDS 30.0%
! TCAATTTGAAGCGTATTGTT+TGG - chr7_2:49533562-49533581 None:intergenic 30.0%
CAACTGAGAAATATTCAAGC+GGG - chr7_2:49532785-49532804 None:intergenic 35.0%
TAGCTAATCTATCACGTTCA+TGG + chr7_2:49532976-49532995 Msa1060840:CDS 35.0%
CTTCTCAATACAGAAAACAC+AGG + chr7_2:49533002-49533021 Msa1060840:CDS 35.0%
ATCAAAATACGCAGTGTGAT+TGG - chr7_2:49533228-49533247 None:intergenic 35.0%
GGAATGTATCTCAAGCAATA+TGG + chr7_2:49533398-49533417 Msa1060840:CDS 35.0%
!! CAGCACAAAGTTTTATATCC+TGG - chr7_2:49533427-49533446 None:intergenic 35.0%
! AACACAATTTGGAATCCTGT+GGG + chr7_2:49533502-49533521 Msa1060840:CDS 35.0%
CAACTGAGAAATATTCAAGC+GGG - chr7_2:49532785-49532804 None:intergenic 35.0%
TAGCTAATCTATCACGTTCA+TGG + chr7_2:49532976-49532995 Msa1060840:CDS 35.0%
CTTCTCAATACAGAAAACAC+AGG + chr7_2:49533002-49533021 Msa1060840:CDS 35.0%
ATCAAAATACGCAGTGTGAT+TGG - chr7_2:49533228-49533247 None:intergenic 35.0%
GGAATGTATCTCAAGCAATA+TGG + chr7_2:49533398-49533417 Msa1060840:CDS 35.0%
!! CAGCACAAAGTTTTATATCC+TGG - chr7_2:49533427-49533446 None:intergenic 35.0%
! AACACAATTTGGAATCCTGT+GGG + chr7_2:49533502-49533521 Msa1060840:CDS 35.0%
GTTAACCCCATGTTAACACA+TGG + chr7_2:49533053-49533072 Msa1060840:CDS 40.0%
TCCAACCATGTGTTAACATG+GGG - chr7_2:49533061-49533080 None:intergenic 40.0%
GTCCAACCATGTGTTAACAT+GGG - chr7_2:49533062-49533081 None:intergenic 40.0%
AGTCCAACCATGTGTTAACA+TGG - chr7_2:49533063-49533082 None:intergenic 40.0%
ACATGGTTGGACTGAGTTTA+AGG + chr7_2:49533070-49533089 Msa1060840:CDS 40.0%
CACCTCATGACTTACATACA+TGG + chr7_2:49533183-49533202 Msa1060840:intron 40.0%
TGCCATGTATGTAAGTCATG+AGG - chr7_2:49533188-49533207 None:intergenic 40.0%
!! TTTTGATTCTCTCCTTACCG+AGG + chr7_2:49533241-49533260 Msa1060840:intron 40.0%
!! CAGCAAGTTGATGACGAATT+TGG + chr7_2:49533377-49533396 Msa1060840:intron 40.0%
TCTCAAGCAATATGGTTTCC+AGG + chr7_2:49533406-49533425 Msa1060840:CDS 40.0%
! CAACACAATTTGGAATCCTG+TGG + chr7_2:49533501-49533520 Msa1060840:CDS 40.0%
AAGACCAACAACTTCAGAGT+TGG + chr7_2:49533536-49533555 Msa1060840:CDS 40.0%
AAGCAATGGAAGATGAAGCA+GGG - chr7_2:49533627-49533646 None:intergenic 40.0%
GTTAACCCCATGTTAACACA+TGG + chr7_2:49533053-49533072 Msa1060840:CDS 40.0%
TCCAACCATGTGTTAACATG+GGG - chr7_2:49533061-49533080 None:intergenic 40.0%
GTCCAACCATGTGTTAACAT+GGG - chr7_2:49533062-49533081 None:intergenic 40.0%
AGTCCAACCATGTGTTAACA+TGG - chr7_2:49533063-49533082 None:intergenic 40.0%
ACATGGTTGGACTGAGTTTA+AGG + chr7_2:49533070-49533089 Msa1060840:CDS 40.0%
CACCTCATGACTTACATACA+TGG + chr7_2:49533183-49533202 Msa1060840:intron 40.0%
TGCCATGTATGTAAGTCATG+AGG - chr7_2:49533188-49533207 None:intergenic 40.0%
!! TTTTGATTCTCTCCTTACCG+AGG + chr7_2:49533241-49533260 Msa1060840:intron 40.0%
!! CAGCAAGTTGATGACGAATT+TGG + chr7_2:49533377-49533396 Msa1060840:intron 40.0%
TCTCAAGCAATATGGTTTCC+AGG + chr7_2:49533406-49533425 Msa1060840:CDS 40.0%
! CAACACAATTTGGAATCCTG+TGG + chr7_2:49533501-49533520 Msa1060840:CDS 40.0%
AAGACCAACAACTTCAGAGT+TGG + chr7_2:49533536-49533555 Msa1060840:CDS 40.0%
AAGCAATGGAAGATGAAGCA+GGG - chr7_2:49533627-49533646 None:intergenic 40.0%
ACCCCATGTTAACACATGGT+TGG + chr7_2:49533057-49533076 Msa1060840:CDS 45.0%
TGAGAAAGTCATCCTCGGTA+AGG - chr7_2:49533256-49533275 None:intergenic 45.0%
TCTTGCAGAAATGATCCCAC+AGG - chr7_2:49533520-49533539 None:intergenic 45.0%
!! TTGGCCAACTCTGAAGTTGT+TGG - chr7_2:49533543-49533562 None:intergenic 45.0%
AGCAATGGAAGATGAAGCAG+GGG - chr7_2:49533626-49533645 None:intergenic 45.0%
GAAGCAATGGAAGATGAAGC+AGG - chr7_2:49533628-49533647 None:intergenic 45.0%
TCCATTGCTTCTGCTTAGTG+AGG + chr7_2:49533637-49533656 Msa1060840:CDS 45.0%
ACCTCACTAAGCAGAAGCAA+TGG - chr7_2:49533641-49533660 None:intergenic 45.0%
ACCCCATGTTAACACATGGT+TGG + chr7_2:49533057-49533076 Msa1060840:CDS 45.0%
TGAGAAAGTCATCCTCGGTA+AGG - chr7_2:49533256-49533275 None:intergenic 45.0%
TCTTGCAGAAATGATCCCAC+AGG - chr7_2:49533520-49533539 None:intergenic 45.0%
!! TTGGCCAACTCTGAAGTTGT+TGG - chr7_2:49533543-49533562 None:intergenic 45.0%
AGCAATGGAAGATGAAGCAG+GGG - chr7_2:49533626-49533645 None:intergenic 45.0%
GAAGCAATGGAAGATGAAGC+AGG - chr7_2:49533628-49533647 None:intergenic 45.0%
TCCATTGCTTCTGCTTAGTG+AGG + chr7_2:49533637-49533656 Msa1060840:CDS 45.0%
ACCTCACTAAGCAGAAGCAA+TGG - chr7_2:49533641-49533660 None:intergenic 45.0%
AGTTGTCAAGGTTGCAGAGC+AGG + chr7_2:49532800-49532819 Msa1060840:CDS 50.0%
TGGGCTGAGAAAGTCATCCT+CGG - chr7_2:49533261-49533280 None:intergenic 50.0%
GCAATGGAAGATGAAGCAGG+GGG - chr7_2:49533625-49533644 None:intergenic 50.0%
AGTTGTCAAGGTTGCAGAGC+AGG + chr7_2:49532800-49532819 Msa1060840:CDS 50.0%
TGGGCTGAGAAAGTCATCCT+CGG - chr7_2:49533261-49533280 None:intergenic 50.0%
GCAATGGAAGATGAAGCAGG+GGG - chr7_2:49533625-49533644 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 49532750 49533667 49532750 ID=Msa1060840;Name=Msa1060840
chr7_2 mRNA 49532750 49533667 49532750 ID=Msa1060840-mRNA-1;Parent=Msa1060840;Name=Msa1060840-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|1|1|3|0|193
chr7_2 exon 49532750 49532821 49532750 ID=Msa1060840-mRNA-1:exon:8918;Parent=Msa1060840-mRNA-1
chr7_2 exon 49532936 49533157 49532936 ID=Msa1060840-mRNA-1:exon:8919;Parent=Msa1060840-mRNA-1
chr7_2 exon 49533380 49533667 49533380 ID=Msa1060840-mRNA-1:exon:8920;Parent=Msa1060840-mRNA-1
chr7_2 CDS 49532750 49532821 49532750 ID=Msa1060840-mRNA-1:cds;Parent=Msa1060840-mRNA-1
chr7_2 CDS 49532936 49533157 49532936 ID=Msa1060840-mRNA-1:cds;Parent=Msa1060840-mRNA-1
chr7_2 CDS 49533380 49533667 49533380 ID=Msa1060840-mRNA-1:cds;Parent=Msa1060840-mRNA-1
Gene Sequence

>Msa1060840

ATGGAAAAAATCAGATCTTTGCTTCAAAGAGACCCGCTTGAATATTTCTCAGTTGTCAAGGTTGCAGAGCAGATGCATCGTGAGATCGAACCAGCTTTTGTGAAGACAAATTTAGCTAATCTATCACGTTCATGGCATCTTCTCAATACAGAAAACACAGGTCACATTTTAACTTTCAACAAAAGCTTGGTTAACCCCATGTTAACACATGGTTGGACTGAGTTTAAGGATTTTAATGATCTTCCTGACAATGTTGAAATTGTTTTTGGTTACTACGGTAATAATATGTTCTCGCAAGTTGATGACGAATTTGGAATGTATCTCAAGCAATATGGTTTCCAGGATATAAAACTTTGTGCTGATAATGGCAATGAAGAGAAATTTAAGGTGTATTTACTTAATGATGGAAAACTTACAACACAATTTGGAATCCTGTGGGATCATTTCTGCAAGACCAACAACTTCAGAGTTGGCCAAACAATACGCTTCAAATTGATGCTTTCAGTTTTAGATAATGTCATGTGTATAAAGTCAATCCCCCTGCTTCATCTTCCATTGCTTCTGCTTAGTGAGGTCAACTAA

Protein sequence

>Msa1060840

MEKIRSLLQRDPLEYFSVVKVAEQMHREIEPAFVKTNLANLSRSWHLLNTENTGHILTFNKSLVNPMLTHGWTEFKDFNDLPDNVEIVFGYYGNNMFSQVDDEFGMYLKQYGFQDIKLCADNGNEEKFKVYLLNDGKLTTQFGILWDHFCKTNNFRVGQTIRFKLMLSVLDNVMCIKSIPLLHLPLLLLSEVN*