AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0608140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608140 MtrunA17_Chr4g0039921 99.107 112 1 0 1 112 1 112 8.65e-77 224
Msa0608140 MtrunA17_Chr4g0039941 78.448 116 25 0 1 116 1 116 2.61e-61 185
Msa0608140 MtrunA17_Chr3g0100201 31.624 117 79 1 1 117 1 116 1.91e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 74 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATACATTGAAGACATTATT+AGG 0.156720 4_2:-55174784 None:intergenic
TCCACCTTCTGAAATCTTTC+AGG 0.221004 4_2:-55173469 None:intergenic
CTTAGATCATAAGAAGAAAA+AGG 0.238310 4_2:-55174610 None:intergenic
ATGTTTATAGTTATATTATA+TGG 0.251963 4_2:-55173202 None:intergenic
TATTGGTAATCAATTAGATT+TGG 0.261773 4_2:+55174747 Msa0608140:three_prime_UTR
CTCTTTGGCACTCTTGCTAT+TGG 0.264799 4_2:+55173424 Msa0608140:CDS
TTTGCTGGAATGCATACTTA+TGG 0.269965 4_2:-55174547 None:intergenic
CCATCTTGTGGGTTACATTT+GGG 0.284338 4_2:-55173161 None:intergenic
TCCATCTTGTGGGTTACATT+TGG 0.298650 4_2:-55173162 None:intergenic
CTCACATAGGTCGACTATAT+CGG 0.323479 4_2:+55174517 Msa0608140:exon
GGGTGTTATGTGTCAAGTTT+AGG 0.343468 4_2:+55173231 Msa0608140:five_prime_UTR
ATTGGTAATCAATTAGATTT+GGG 0.345510 4_2:+55174748 Msa0608140:three_prime_UTR
ATCTAATTGATTACCAATAG+TGG 0.351675 4_2:-55174743 None:intergenic
GTGGGTTGAGTTTCCACTAT+TGG 0.354685 4_2:+55174730 Msa0608140:three_prime_UTR
TTGCTGGAATGCATACTTAT+GGG 0.367273 4_2:-55174546 None:intergenic
TTGACAAGGAATGATGTCTT+TGG 0.371217 4_2:-55174818 None:intergenic
AGATCCCATGCTTCACTTGC+TGG 0.373899 4_2:-55173403 None:intergenic
ATACATTGAAGACATTATTA+GGG 0.379462 4_2:-55174783 None:intergenic
GACATATAACATATATTTGC+TGG 0.380503 4_2:-55174562 None:intergenic
AAAGAGATCAAGAAGATGTT+TGG 0.416699 4_2:+55173349 Msa0608140:exon
TCAGGTTTACCTGGCCCAAC+TGG 0.416975 4_2:+55174322 Msa0608140:intron
TTGAAGGAGATGGAGGGGTT+GGG 0.418155 4_2:+55173497 Msa0608140:CDS
CTTGAGTAGCATCAAGTCTT+GGG 0.440551 4_2:-55174706 None:intergenic
AGTGAAGCATGGGATCTCTT+TGG 0.449536 4_2:+55173409 Msa0608140:CDS
ATTGAAGGAGATGGAGGGGT+TGG 0.457658 4_2:+55173496 Msa0608140:CDS
ATAATATAACTATAAACATC+TGG 0.459178 4_2:+55173206 Msa0608140:five_prime_UTR
TGGCCGCTTCACGGAGTTCA+AGG 0.460923 4_2:-55174485 None:intergenic
AAAGAAGTGGAGGTGGTTGA+AGG 0.462385 4_2:+55174388 Msa0608140:CDS
ACCTATGTGAGAATGAAGTT+TGG 0.466094 4_2:-55174505 None:intergenic
ACTTGAGTAGCATCAAGTCT+TGG 0.480235 4_2:-55174707 None:intergenic
TACACAAACTATTTGGGATG+TGG 0.481481 4_2:-55173326 None:intergenic
AAGAAGTGGAGGTGGTTGAA+GGG 0.482356 4_2:+55174389 Msa0608140:CDS
TCACATAGGTCGACTATATC+GGG 0.483659 4_2:+55174518 Msa0608140:exon
GGAGTTTGATCAGGTTTACC+TGG 0.484092 4_2:+55174313 Msa0608140:intron
TTTGCATGCCTCCATCTTGT+GGG 0.487297 4_2:-55173172 None:intergenic
GCATCAAGTCTTGGGTTCAA+TGG 0.488435 4_2:-55174698 None:intergenic
AATGAAGTTTGGCCGCTTCA+CGG 0.489539 4_2:-55174494 None:intergenic
TATGTTATATGTCCATTAAG+AGG 0.489898 4_2:+55174572 Msa0608140:three_prime_UTR
CACACTATTTAGAGTTCGTA+TGG 0.490157 4_2:+55174432 Msa0608140:CDS
TATAACTATAAACATCTGGC+AGG 0.492572 4_2:+55173210 Msa0608140:five_prime_UTR
CGTATGGAAGTGATAGAGAA+AGG 0.493358 4_2:+55174448 Msa0608140:CDS
CAGAAGGTGGAGCTAATTGA+AGG 0.500124 4_2:+55173481 Msa0608140:CDS
AGAAGTGGAGGTGGTTGAAG+GGG 0.506956 4_2:+55174390 Msa0608140:CDS
GTGGAGCTAATTGAAGGAGA+TGG 0.515580 4_2:+55173487 Msa0608140:CDS
CTCAAACTCACATTCACTCC+AGG 0.525858 4_2:+55173526 Msa0608140:CDS
TGGCACTCTTGCTATTGGAA+AGG 0.531710 4_2:+55173429 Msa0608140:CDS
GAGCTAATTGAAGGAGATGG+AGG 0.540375 4_2:+55173490 Msa0608140:CDS
TGAAGACATTATTAGGGATG+TGG 0.552287 4_2:-55174777 None:intergenic
GAAGTGGAGGTGGTTGAAGG+GGG 0.553306 4_2:+55174391 Msa0608140:CDS
CGTGCCAGCAAGTGAAGCAT+GGG 0.555967 4_2:+55173399 Msa0608140:CDS
CCCAAATGTAACCCACAAGA+TGG 0.560304 4_2:+55173161 Msa0608140:five_prime_UTR
GTCCCTTGAACTCCGTGAAG+CGG 0.561138 4_2:+55174482 Msa0608140:CDS
TGAAAAGCACATCAAAGAAG+TGG 0.563701 4_2:+55174375 Msa0608140:CDS
ACGTGCCAGCAAGTGAAGCA+TGG 0.570168 4_2:+55173398 Msa0608140:CDS
TATACTTCACATCAAAATTG+TGG 0.571099 4_2:+55173263 Msa0608140:five_prime_UTR
GCACATCAAAGAAGTGGAGG+TGG 0.578278 4_2:+55174381 Msa0608140:CDS
AGCTAATTGAAGGAGATGGA+GGG 0.580245 4_2:+55173491 Msa0608140:CDS
TATTTGGGATGTGGTGTATG+TGG 0.581504 4_2:-55173317 None:intergenic
GCTAATTGAAGGAGATGGAG+GGG 0.586950 4_2:+55173492 Msa0608140:CDS
GGCCGCTTCACGGAGTTCAA+GGG 0.589585 4_2:-55174484 None:intergenic
GACTTGATGCTACTCAAGTG+TGG 0.592124 4_2:+55174711 Msa0608140:three_prime_UTR
TGCTGGAATGCATACTTATG+GGG 0.594239 4_2:-55174545 None:intergenic
GCCTGAAAGATTTCAGAAGG+TGG 0.605828 4_2:+55173468 Msa0608140:CDS
TGCTATTGGAAAGGTCGTTG+AGG 0.616559 4_2:+55173438 Msa0608140:CDS
ATAACTATAAACATCTGGCA+GGG 0.617981 4_2:+55173211 Msa0608140:five_prime_UTR
GACGATAGATAACATCCCAA+GGG 0.621230 4_2:-55174642 None:intergenic
TGACGATAGATAACATCCCA+AGG 0.622633 4_2:-55174643 None:intergenic
GATGCCTGAAAGATTTCAGA+AGG 0.633462 4_2:+55173465 Msa0608140:CDS
GATGTGGTGTATGTGGTGCG+TGG 0.649817 4_2:-55173310 None:intergenic
AAAGCACATCAAAGAAGTGG+AGG 0.653324 4_2:+55174378 Msa0608140:CDS
GCCAAACTTCATTCTCACAT+AGG 0.653610 4_2:+55174504 Msa0608140:CDS
ACTTGATGCTACTCAAGTGT+GGG 0.668480 4_2:+55174712 Msa0608140:three_prime_UTR
AAATGTAACCCACAAGATGG+AGG 0.674240 4_2:+55173164 Msa0608140:five_prime_UTR
ACGATAGATAACATCCCAAG+GGG 0.723011 4_2:-55174641 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTTATAGTTATATTATA+TGG - chr4_2:55173205-55173224 None:intergenic 10.0%
!! ATGTTTATAGTTATATTATA+TGG - chr4_2:55173205-55173224 None:intergenic 10.0%
!! ATAATATAACTATAAACATC+TGG + chr4_2:55173206-55173225 Msa0608140:five_prime_UTR 15.0%
!! AAATAATAATGAAAATAACC+TGG - chr4_2:55173547-55173566 None:intergenic 15.0%
!!! TATATTTTTGAGTATAATTG+TGG - chr4_2:55173592-55173611 None:intergenic 15.0%
!! ATTATTTACACTTGAATAAA+AGG - chr4_2:55174013-55174032 None:intergenic 15.0%
!!! TTTTATTCAAGTGTAAATAA+TGG + chr4_2:55174012-55174031 Msa0608140:intron 15.0%
!!! TAACATAACATTAATTGTTT+TGG + chr4_2:55174288-55174307 Msa0608140:intron 15.0%
!! ATAATATAACTATAAACATC+TGG + chr4_2:55173206-55173225 Msa0608140:five_prime_UTR 15.0%
!! AAATAATAATGAAAATAACC+TGG - chr4_2:55173547-55173566 None:intergenic 15.0%
!!! TATATTTTTGAGTATAATTG+TGG - chr4_2:55173592-55173611 None:intergenic 15.0%
!! ATTATTTACACTTGAATAAA+AGG - chr4_2:55174013-55174032 None:intergenic 15.0%
!!! TTTTATTCAAGTGTAAATAA+TGG + chr4_2:55174012-55174031 Msa0608140:intron 15.0%
!!! TAACATAACATTAATTGTTT+TGG + chr4_2:55174288-55174307 Msa0608140:intron 15.0%
!! TTATCGTTACATAATTCATA+TGG + chr4_2:55173613-55173632 Msa0608140:intron 20.0%
!! AATTAAATAGAAAGTAGTCT+CGG - chr4_2:55173827-55173846 None:intergenic 20.0%
!! ATGATGAATTAACATACTTA+CGG + chr4_2:55174192-55174211 Msa0608140:intron 20.0%
!!! ATAACATTAATTGTTTTGGT+CGG + chr4_2:55174292-55174311 Msa0608140:intron 20.0%
!! TTAGATCATAAGAAGAAAAA+GGG - chr4_2:55174612-55174631 None:intergenic 20.0%
!!! TATTGGTAATCAATTAGATT+TGG + chr4_2:55174747-55174766 Msa0608140:three_prime_UTR 20.0%
!!! ATTGGTAATCAATTAGATTT+GGG + chr4_2:55174748-55174767 Msa0608140:three_prime_UTR 20.0%
!! ATACATTGAAGACATTATTA+GGG - chr4_2:55174786-55174805 None:intergenic 20.0%
!! AATACATTGAAGACATTATT+AGG - chr4_2:55174787-55174806 None:intergenic 20.0%
!! TTATCGTTACATAATTCATA+TGG + chr4_2:55173613-55173632 Msa0608140:intron 20.0%
!! AATTAAATAGAAAGTAGTCT+CGG - chr4_2:55173827-55173846 None:intergenic 20.0%
!! ATGATGAATTAACATACTTA+CGG + chr4_2:55174192-55174211 Msa0608140:intron 20.0%
!!! ATAACATTAATTGTTTTGGT+CGG + chr4_2:55174292-55174311 Msa0608140:intron 20.0%
!! TTAGATCATAAGAAGAAAAA+GGG - chr4_2:55174612-55174631 None:intergenic 20.0%
!!! TATTGGTAATCAATTAGATT+TGG + chr4_2:55174747-55174766 Msa0608140:three_prime_UTR 20.0%
!!! ATTGGTAATCAATTAGATTT+GGG + chr4_2:55174748-55174767 Msa0608140:three_prime_UTR 20.0%
!! ATACATTGAAGACATTATTA+GGG - chr4_2:55174786-55174805 None:intergenic 20.0%
!! AATACATTGAAGACATTATT+AGG - chr4_2:55174787-55174806 None:intergenic 20.0%
! TATACTTCACATCAAAATTG+TGG + chr4_2:55173263-55173282 Msa0608140:five_prime_UTR 25.0%
!! CTCTTTTACACAAACTATTT+GGG - chr4_2:55173335-55173354 None:intergenic 25.0%
!! TCTCTTTTACACAAACTATT+TGG - chr4_2:55173336-55173355 None:intergenic 25.0%
!! CTATTGTATCATGAACATTT+TGG + chr4_2:55173864-55173883 Msa0608140:intron 25.0%
! AATCTATAGATTCATGAGTT+TGG + chr4_2:55174149-55174168 Msa0608140:intron 25.0%
! TGATGAATTAACATACTTAC+GGG + chr4_2:55174193-55174212 Msa0608140:intron 25.0%
!! ACTTTTCTTTTAAACCAGTT+GGG - chr4_2:55174339-55174358 None:intergenic 25.0%
!! AACTTTTCTTTTAAACCAGT+TGG - chr4_2:55174340-55174359 None:intergenic 25.0%
! GACATATAACATATATTTGC+TGG - chr4_2:55174565-55174584 None:intergenic 25.0%
! TATGTTATATGTCCATTAAG+AGG + chr4_2:55174572-55174591 Msa0608140:three_prime_UTR 25.0%
! CTTAGATCATAAGAAGAAAA+AGG - chr4_2:55174613-55174632 None:intergenic 25.0%
! ATCTAATTGATTACCAATAG+TGG - chr4_2:55174746-55174765 None:intergenic 25.0%
! TATACTTCACATCAAAATTG+TGG + chr4_2:55173263-55173282 Msa0608140:five_prime_UTR 25.0%
!! CTCTTTTACACAAACTATTT+GGG - chr4_2:55173335-55173354 None:intergenic 25.0%
!! TCTCTTTTACACAAACTATT+TGG - chr4_2:55173336-55173355 None:intergenic 25.0%
!! CTATTGTATCATGAACATTT+TGG + chr4_2:55173864-55173883 Msa0608140:intron 25.0%
! AATCTATAGATTCATGAGTT+TGG + chr4_2:55174149-55174168 Msa0608140:intron 25.0%
! TGATGAATTAACATACTTAC+GGG + chr4_2:55174193-55174212 Msa0608140:intron 25.0%
!! ACTTTTCTTTTAAACCAGTT+GGG - chr4_2:55174339-55174358 None:intergenic 25.0%
!! AACTTTTCTTTTAAACCAGT+TGG - chr4_2:55174340-55174359 None:intergenic 25.0%
! GACATATAACATATATTTGC+TGG - chr4_2:55174565-55174584 None:intergenic 25.0%
! TATGTTATATGTCCATTAAG+AGG + chr4_2:55174572-55174591 Msa0608140:three_prime_UTR 25.0%
! CTTAGATCATAAGAAGAAAA+AGG - chr4_2:55174613-55174632 None:intergenic 25.0%
! ATCTAATTGATTACCAATAG+TGG - chr4_2:55174746-55174765 None:intergenic 25.0%
TATAACTATAAACATCTGGC+AGG + chr4_2:55173210-55173229 Msa0608140:five_prime_UTR 30.0%
ATAACTATAAACATCTGGCA+GGG + chr4_2:55173211-55173230 Msa0608140:five_prime_UTR 30.0%
AAAGAGATCAAGAAGATGTT+TGG + chr4_2:55173349-55173368 Msa0608140:exon 30.0%
TTCATCTTAAGCTGAATAAG+TGG + chr4_2:55173734-55173753 Msa0608140:intron 30.0%
ACATTTGATATGTAAGGATC+AGG - chr4_2:55173776-55173795 None:intergenic 30.0%
GACATCACATTTGATATGTA+AGG - chr4_2:55173782-55173801 None:intergenic 30.0%
!! ATTGATTTTGCCATCCAAAA+AGG + chr4_2:55173959-55173978 Msa0608140:intron 30.0%
! GTGCTTTTCATTATCAATCT+TGG - chr4_2:55174365-55174384 None:intergenic 30.0%
ATCATAAGAAGAAAAAGGGT+AGG - chr4_2:55174608-55174627 None:intergenic 30.0%
TATAACTATAAACATCTGGC+AGG + chr4_2:55173210-55173229 Msa0608140:five_prime_UTR 30.0%
ATAACTATAAACATCTGGCA+GGG + chr4_2:55173211-55173230 Msa0608140:five_prime_UTR 30.0%
AAAGAGATCAAGAAGATGTT+TGG + chr4_2:55173349-55173368 Msa0608140:exon 30.0%
TTCATCTTAAGCTGAATAAG+TGG + chr4_2:55173734-55173753 Msa0608140:intron 30.0%
ACATTTGATATGTAAGGATC+AGG - chr4_2:55173776-55173795 None:intergenic 30.0%
GACATCACATTTGATATGTA+AGG - chr4_2:55173782-55173801 None:intergenic 30.0%
!! ATTGATTTTGCCATCCAAAA+AGG + chr4_2:55173959-55173978 Msa0608140:intron 30.0%
! GTGCTTTTCATTATCAATCT+TGG - chr4_2:55174365-55174384 None:intergenic 30.0%
ATCATAAGAAGAAAAAGGGT+AGG - chr4_2:55174608-55174627 None:intergenic 30.0%
! TACACAAACTATTTGGGATG+TGG - chr4_2:55173329-55173348 None:intergenic 35.0%
GAGGATGTGTTTACAATTGA+TGG - chr4_2:55173660-55173679 None:intergenic 35.0%
AAATGTGATGTCCCTAACAA+TGG + chr4_2:55173790-55173809 Msa0608140:intron 35.0%
ATAACAATGTCATGTCTCCT+AGG + chr4_2:55173912-55173931 Msa0608140:intron 35.0%
ACACTTGAATAAAAGGCAAG+CGG - chr4_2:55174006-55174025 None:intergenic 35.0%
CATTATGTCTATGTACAGGT+GGG + chr4_2:55174109-55174128 Msa0608140:intron 35.0%
ATTATGTCTATGTACAGGTG+GGG + chr4_2:55174110-55174129 Msa0608140:intron 35.0%
! TGAAAAGCACATCAAAGAAG+TGG + chr4_2:55174375-55174394 Msa0608140:CDS 35.0%
! CACACTATTTAGAGTTCGTA+TGG + chr4_2:55174432-55174451 Msa0608140:CDS 35.0%
ACCTATGTGAGAATGAAGTT+TGG - chr4_2:55174508-55174527 None:intergenic 35.0%
TTGCTGGAATGCATACTTAT+GGG - chr4_2:55174549-55174568 None:intergenic 35.0%
TTTGCTGGAATGCATACTTA+TGG - chr4_2:55174550-55174569 None:intergenic 35.0%
! ATCTAAGTACTTTTACCCCT+TGG + chr4_2:55174626-55174645 Msa0608140:three_prime_UTR 35.0%
! TCTAAGTACTTTTACCCCTT+GGG + chr4_2:55174627-55174646 Msa0608140:three_prime_UTR 35.0%
TGAAGACATTATTAGGGATG+TGG - chr4_2:55174780-55174799 None:intergenic 35.0%
! TACACAAACTATTTGGGATG+TGG - chr4_2:55173329-55173348 None:intergenic 35.0%
GAGGATGTGTTTACAATTGA+TGG - chr4_2:55173660-55173679 None:intergenic 35.0%
AAATGTGATGTCCCTAACAA+TGG + chr4_2:55173790-55173809 Msa0608140:intron 35.0%
ATAACAATGTCATGTCTCCT+AGG + chr4_2:55173912-55173931 Msa0608140:intron 35.0%
ACACTTGAATAAAAGGCAAG+CGG - chr4_2:55174006-55174025 None:intergenic 35.0%
CATTATGTCTATGTACAGGT+GGG + chr4_2:55174109-55174128 Msa0608140:intron 35.0%
ATTATGTCTATGTACAGGTG+GGG + chr4_2:55174110-55174129 Msa0608140:intron 35.0%
! TGAAAAGCACATCAAAGAAG+TGG + chr4_2:55174375-55174394 Msa0608140:CDS 35.0%
! CACACTATTTAGAGTTCGTA+TGG + chr4_2:55174432-55174451 Msa0608140:CDS 35.0%
ACCTATGTGAGAATGAAGTT+TGG - chr4_2:55174508-55174527 None:intergenic 35.0%
TTGCTGGAATGCATACTTAT+GGG - chr4_2:55174549-55174568 None:intergenic 35.0%
TTTGCTGGAATGCATACTTA+TGG - chr4_2:55174550-55174569 None:intergenic 35.0%
! ATCTAAGTACTTTTACCCCT+TGG + chr4_2:55174626-55174645 Msa0608140:three_prime_UTR 35.0%
! TCTAAGTACTTTTACCCCTT+GGG + chr4_2:55174627-55174646 Msa0608140:three_prime_UTR 35.0%
TGAAGACATTATTAGGGATG+TGG - chr4_2:55174780-55174799 None:intergenic 35.0%
! CCATCTTGTGGGTTACATTT+GGG - chr4_2:55173164-55173183 None:intergenic 40.0%
! TCCATCTTGTGGGTTACATT+TGG - chr4_2:55173165-55173184 None:intergenic 40.0%
AAATGTAACCCACAAGATGG+AGG + chr4_2:55173164-55173183 Msa0608140:five_prime_UTR 40.0%
! GGGTGTTATGTGTCAAGTTT+AGG + chr4_2:55173231-55173250 Msa0608140:five_prime_UTR 40.0%
! TATTTGGGATGTGGTGTATG+TGG - chr4_2:55173320-55173339 None:intergenic 40.0%
GATGCCTGAAAGATTTCAGA+AGG + chr4_2:55173465-55173484 Msa0608140:CDS 40.0%
!! TCCACCTTCTGAAATCTTTC+AGG - chr4_2:55173472-55173491 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_2:55173491-55173510 Msa0608140:CDS 40.0%
TAACCACCTTATTCTCCACT+AGG - chr4_2:55173697-55173716 None:intergenic 40.0%
AATAGAAAGTAGTCTCGGCA+AGG - chr4_2:55173822-55173841 None:intergenic 40.0%
AACACAACATTCATGGACCT+AGG - chr4_2:55173932-55173951 None:intergenic 40.0%
CATCGTGAACACAACATTCA+TGG - chr4_2:55173939-55173958 None:intergenic 40.0%
CACTTGAATAAAAGGCAAGC+GGG - chr4_2:55174005-55174024 None:intergenic 40.0%
CCTGTACATAGACATAATGG+TGG - chr4_2:55174108-55174127 None:intergenic 40.0%
CCACCATTATGTCTATGTAC+AGG + chr4_2:55174105-55174124 Msa0608140:intron 40.0%
CCACCTGTACATAGACATAA+TGG - chr4_2:55174111-55174130 None:intergenic 40.0%
CCATTATGTCTATGTACAGG+TGG + chr4_2:55174108-55174127 Msa0608140:intron 40.0%
TTATGTCTATGTACAGGTGG+GGG + chr4_2:55174111-55174130 Msa0608140:intron 40.0%
AAAGCACATCAAAGAAGTGG+AGG + chr4_2:55174378-55174397 Msa0608140:CDS 40.0%
CGTATGGAAGTGATAGAGAA+AGG + chr4_2:55174448-55174467 Msa0608140:CDS 40.0%
GCCAAACTTCATTCTCACAT+AGG + chr4_2:55174504-55174523 Msa0608140:CDS 40.0%
CTCACATAGGTCGACTATAT+CGG + chr4_2:55174517-55174536 Msa0608140:exon 40.0%
TCACATAGGTCGACTATATC+GGG + chr4_2:55174518-55174537 Msa0608140:exon 40.0%
TGCTGGAATGCATACTTATG+GGG - chr4_2:55174548-55174567 None:intergenic 40.0%
! GGATGAGGTTTTCCTCTTAA+TGG - chr4_2:55174587-55174606 None:intergenic 40.0%
AGAAGAAAAAGGGTAGGATG+AGG - chr4_2:55174602-55174621 None:intergenic 40.0%
ACGATAGATAACATCCCAAG+GGG - chr4_2:55174644-55174663 None:intergenic 40.0%
GACGATAGATAACATCCCAA+GGG - chr4_2:55174645-55174664 None:intergenic 40.0%
TGACGATAGATAACATCCCA+AGG - chr4_2:55174646-55174665 None:intergenic 40.0%
CTTGAGTAGCATCAAGTCTT+GGG - chr4_2:55174709-55174728 None:intergenic 40.0%
ACTTGAGTAGCATCAAGTCT+TGG - chr4_2:55174710-55174729 None:intergenic 40.0%
ACTTGATGCTACTCAAGTGT+GGG + chr4_2:55174712-55174731 Msa0608140:three_prime_UTR 40.0%
! ATCTTGTGGGTTACATTTGG+GGG - chr4_2:55173162-55173181 None:intergenic 40.0%
! CATCTTGTGGGTTACATTTG+GGG - chr4_2:55173163-55173182 None:intergenic 40.0%
! CCATCTTGTGGGTTACATTT+GGG - chr4_2:55173164-55173183 None:intergenic 40.0%
! TCCATCTTGTGGGTTACATT+TGG - chr4_2:55173165-55173184 None:intergenic 40.0%
AAATGTAACCCACAAGATGG+AGG + chr4_2:55173164-55173183 Msa0608140:five_prime_UTR 40.0%
! GGGTGTTATGTGTCAAGTTT+AGG + chr4_2:55173231-55173250 Msa0608140:five_prime_UTR 40.0%
! TATTTGGGATGTGGTGTATG+TGG - chr4_2:55173320-55173339 None:intergenic 40.0%
GATGCCTGAAAGATTTCAGA+AGG + chr4_2:55173465-55173484 Msa0608140:CDS 40.0%
!! TCCACCTTCTGAAATCTTTC+AGG - chr4_2:55173472-55173491 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_2:55173491-55173510 Msa0608140:CDS 40.0%
TAACCACCTTATTCTCCACT+AGG - chr4_2:55173697-55173716 None:intergenic 40.0%
AATAGAAAGTAGTCTCGGCA+AGG - chr4_2:55173822-55173841 None:intergenic 40.0%
AACACAACATTCATGGACCT+AGG - chr4_2:55173932-55173951 None:intergenic 40.0%
CATCGTGAACACAACATTCA+TGG - chr4_2:55173939-55173958 None:intergenic 40.0%
CACTTGAATAAAAGGCAAGC+GGG - chr4_2:55174005-55174024 None:intergenic 40.0%
CCTGTACATAGACATAATGG+TGG - chr4_2:55174108-55174127 None:intergenic 40.0%
CCACCATTATGTCTATGTAC+AGG + chr4_2:55174105-55174124 Msa0608140:intron 40.0%
CCACCTGTACATAGACATAA+TGG - chr4_2:55174111-55174130 None:intergenic 40.0%
CCATTATGTCTATGTACAGG+TGG + chr4_2:55174108-55174127 Msa0608140:intron 40.0%
TTATGTCTATGTACAGGTGG+GGG + chr4_2:55174111-55174130 Msa0608140:intron 40.0%
AAAGCACATCAAAGAAGTGG+AGG + chr4_2:55174378-55174397 Msa0608140:CDS 40.0%
CGTATGGAAGTGATAGAGAA+AGG + chr4_2:55174448-55174467 Msa0608140:CDS 40.0%
GCCAAACTTCATTCTCACAT+AGG + chr4_2:55174504-55174523 Msa0608140:CDS 40.0%
CTCACATAGGTCGACTATAT+CGG + chr4_2:55174517-55174536 Msa0608140:exon 40.0%
TCACATAGGTCGACTATATC+GGG + chr4_2:55174518-55174537 Msa0608140:exon 40.0%
TGCTGGAATGCATACTTATG+GGG - chr4_2:55174548-55174567 None:intergenic 40.0%
! GGATGAGGTTTTCCTCTTAA+TGG - chr4_2:55174587-55174606 None:intergenic 40.0%
AGAAGAAAAAGGGTAGGATG+AGG - chr4_2:55174602-55174621 None:intergenic 40.0%
ACGATAGATAACATCCCAAG+GGG - chr4_2:55174644-55174663 None:intergenic 40.0%
GACGATAGATAACATCCCAA+GGG - chr4_2:55174645-55174664 None:intergenic 40.0%
TGACGATAGATAACATCCCA+AGG - chr4_2:55174646-55174665 None:intergenic 40.0%
CTTGAGTAGCATCAAGTCTT+GGG - chr4_2:55174709-55174728 None:intergenic 40.0%
ACTTGAGTAGCATCAAGTCT+TGG - chr4_2:55174710-55174729 None:intergenic 40.0%
ACTTGATGCTACTCAAGTGT+GGG + chr4_2:55174712-55174731 Msa0608140:three_prime_UTR 40.0%
CCCAAATGTAACCCACAAGA+TGG + chr4_2:55173161-55173180 Msa0608140:five_prime_UTR 45.0%
TTTGCATGCCTCCATCTTGT+GGG - chr4_2:55173175-55173194 None:intergenic 45.0%
! TTTTGCATGCCTCCATCTTG+TGG - chr4_2:55173176-55173195 None:intergenic 45.0%
AGTGAAGCATGGGATCTCTT+TGG + chr4_2:55173409-55173428 Msa0608140:CDS 45.0%
! CTCTTTGGCACTCTTGCTAT+TGG + chr4_2:55173424-55173443 Msa0608140:CDS 45.0%
!! TGGCACTCTTGCTATTGGAA+AGG + chr4_2:55173429-55173448 Msa0608140:CDS 45.0%
TGCTATTGGAAAGGTCGTTG+AGG + chr4_2:55173438-55173457 Msa0608140:CDS 45.0%
GCCTGAAAGATTTCAGAAGG+TGG + chr4_2:55173468-55173487 Msa0608140:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_2:55173481-55173500 Msa0608140:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_2:55173487-55173506 Msa0608140:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_2:55173490-55173509 Msa0608140:CDS 45.0%
GCTAATTGAAGGAGATGGAG+GGG + chr4_2:55173492-55173511 Msa0608140:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_2:55173526-55173545 Msa0608140:CDS 45.0%
TGTAAACACATCCTCCGTTG+AGG + chr4_2:55173665-55173684 Msa0608140:intron 45.0%
CGTTGACCTAGTGGAGAATA+AGG + chr4_2:55173688-55173707 Msa0608140:intron 45.0%
TGACCTAGTGGAGAATAAGG+TGG + chr4_2:55173691-55173710 Msa0608140:intron 45.0%
CAAGGATCGCTCCATTGTTA+GGG - chr4_2:55173804-55173823 None:intergenic 45.0%
TGCCATCCAAAAAGGTCTAG+CGG + chr4_2:55173967-55173986 Msa0608140:intron 45.0%
!! GTTTTGGTCGGAGTTTGATC+AGG + chr4_2:55174304-55174323 Msa0608140:intron 45.0%
GGAGTTTGATCAGGTTTACC+TGG + chr4_2:55174313-55174332 Msa0608140:intron 45.0%
! TCTTTTAAACCAGTTGGGCC+AGG - chr4_2:55174334-55174353 None:intergenic 45.0%
AAAGAAGTGGAGGTGGTTGA+AGG + chr4_2:55174388-55174407 Msa0608140:CDS 45.0%
AAGAAGTGGAGGTGGTTGAA+GGG + chr4_2:55174389-55174408 Msa0608140:CDS 45.0%
AATGAAGTTTGGCCGCTTCA+CGG - chr4_2:55174497-55174516 None:intergenic 45.0%
GCATCAAGTCTTGGGTTCAA+TGG - chr4_2:55174701-55174720 None:intergenic 45.0%
GACTTGATGCTACTCAAGTG+TGG + chr4_2:55174711-55174730 Msa0608140:three_prime_UTR 45.0%
! GTGGGTTGAGTTTCCACTAT+TGG + chr4_2:55174730-55174749 Msa0608140:three_prime_UTR 45.0%
CCCAAATGTAACCCACAAGA+TGG + chr4_2:55173161-55173180 Msa0608140:five_prime_UTR 45.0%
TTTGCATGCCTCCATCTTGT+GGG - chr4_2:55173175-55173194 None:intergenic 45.0%
! TTTTGCATGCCTCCATCTTG+TGG - chr4_2:55173176-55173195 None:intergenic 45.0%
AGTGAAGCATGGGATCTCTT+TGG + chr4_2:55173409-55173428 Msa0608140:CDS 45.0%
! CTCTTTGGCACTCTTGCTAT+TGG + chr4_2:55173424-55173443 Msa0608140:CDS 45.0%
!! TGGCACTCTTGCTATTGGAA+AGG + chr4_2:55173429-55173448 Msa0608140:CDS 45.0%
TGCTATTGGAAAGGTCGTTG+AGG + chr4_2:55173438-55173457 Msa0608140:CDS 45.0%
GCCTGAAAGATTTCAGAAGG+TGG + chr4_2:55173468-55173487 Msa0608140:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_2:55173481-55173500 Msa0608140:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_2:55173487-55173506 Msa0608140:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_2:55173490-55173509 Msa0608140:CDS 45.0%
GCTAATTGAAGGAGATGGAG+GGG + chr4_2:55173492-55173511 Msa0608140:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_2:55173526-55173545 Msa0608140:CDS 45.0%
TGTAAACACATCCTCCGTTG+AGG + chr4_2:55173665-55173684 Msa0608140:intron 45.0%
CGTTGACCTAGTGGAGAATA+AGG + chr4_2:55173688-55173707 Msa0608140:intron 45.0%
TGACCTAGTGGAGAATAAGG+TGG + chr4_2:55173691-55173710 Msa0608140:intron 45.0%
CAAGGATCGCTCCATTGTTA+GGG - chr4_2:55173804-55173823 None:intergenic 45.0%
TGCCATCCAAAAAGGTCTAG+CGG + chr4_2:55173967-55173986 Msa0608140:intron 45.0%
!! GTTTTGGTCGGAGTTTGATC+AGG + chr4_2:55174304-55174323 Msa0608140:intron 45.0%
GGAGTTTGATCAGGTTTACC+TGG + chr4_2:55174313-55174332 Msa0608140:intron 45.0%
! TCTTTTAAACCAGTTGGGCC+AGG - chr4_2:55174334-55174353 None:intergenic 45.0%
AAAGAAGTGGAGGTGGTTGA+AGG + chr4_2:55174388-55174407 Msa0608140:CDS 45.0%
AAGAAGTGGAGGTGGTTGAA+GGG + chr4_2:55174389-55174408 Msa0608140:CDS 45.0%
AATGAAGTTTGGCCGCTTCA+CGG - chr4_2:55174497-55174516 None:intergenic 45.0%
GCATCAAGTCTTGGGTTCAA+TGG - chr4_2:55174701-55174720 None:intergenic 45.0%
GACTTGATGCTACTCAAGTG+TGG + chr4_2:55174711-55174730 Msa0608140:three_prime_UTR 45.0%
! GTGGGTTGAGTTTCCACTAT+TGG + chr4_2:55174730-55174749 Msa0608140:three_prime_UTR 45.0%
AGATCCCATGCTTCACTTGC+TGG - chr4_2:55173406-55173425 None:intergenic 50.0%
ATTGAAGGAGATGGAGGGGT+TGG + chr4_2:55173496-55173515 Msa0608140:CDS 50.0%
TTGAAGGAGATGGAGGGGTT+GGG + chr4_2:55173497-55173516 Msa0608140:CDS 50.0%
GCAAGGATCGCTCCATTGTT+AGG - chr4_2:55173805-55173824 None:intergenic 50.0%
CTATGTACAGGTGGGGGATT+CGG + chr4_2:55174117-55174136 Msa0608140:intron 50.0%
GCACATCAAAGAAGTGGAGG+TGG + chr4_2:55174381-55174400 Msa0608140:CDS 50.0%
AGAAGTGGAGGTGGTTGAAG+GGG + chr4_2:55174390-55174409 Msa0608140:CDS 50.0%
GAAGGGGGATGCTTAGATCT+TGG + chr4_2:55174406-55174425 Msa0608140:CDS 50.0%
AGATCCCATGCTTCACTTGC+TGG - chr4_2:55173406-55173425 None:intergenic 50.0%
ATTGAAGGAGATGGAGGGGT+TGG + chr4_2:55173496-55173515 Msa0608140:CDS 50.0%
TTGAAGGAGATGGAGGGGTT+GGG + chr4_2:55173497-55173516 Msa0608140:CDS 50.0%
GCAAGGATCGCTCCATTGTT+AGG - chr4_2:55173805-55173824 None:intergenic 50.0%
CTATGTACAGGTGGGGGATT+CGG + chr4_2:55174117-55174136 Msa0608140:intron 50.0%
GCACATCAAAGAAGTGGAGG+TGG + chr4_2:55174381-55174400 Msa0608140:CDS 50.0%
AGAAGTGGAGGTGGTTGAAG+GGG + chr4_2:55174390-55174409 Msa0608140:CDS 50.0%
GAAGGGGGATGCTTAGATCT+TGG + chr4_2:55174406-55174425 Msa0608140:CDS 50.0%
GATGTGGTGTATGTGGTGCG+TGG - chr4_2:55173313-55173332 None:intergenic 55.0%
ACGTGCCAGCAAGTGAAGCA+TGG + chr4_2:55173398-55173417 Msa0608140:CDS 55.0%
CGTGCCAGCAAGTGAAGCAT+GGG + chr4_2:55173399-55173418 Msa0608140:CDS 55.0%
CCACTAGGTCAACGCCTCAA+CGG - chr4_2:55173682-55173701 None:intergenic 55.0%
! CGCCGCTAGACCTTTTTGGA+TGG - chr4_2:55173972-55173991 None:intergenic 55.0%
TCAGGTTTACCTGGCCCAAC+TGG + chr4_2:55174322-55174341 Msa0608140:intron 55.0%
GAAGTGGAGGTGGTTGAAGG+GGG + chr4_2:55174391-55174410 Msa0608140:CDS 55.0%
GTCCCTTGAACTCCGTGAAG+CGG + chr4_2:55174482-55174501 Msa0608140:CDS 55.0%
GATGTGGTGTATGTGGTGCG+TGG - chr4_2:55173313-55173332 None:intergenic 55.0%
ACGTGCCAGCAAGTGAAGCA+TGG + chr4_2:55173398-55173417 Msa0608140:CDS 55.0%
CGTGCCAGCAAGTGAAGCAT+GGG + chr4_2:55173399-55173418 Msa0608140:CDS 55.0%
CCACTAGGTCAACGCCTCAA+CGG - chr4_2:55173682-55173701 None:intergenic 55.0%
! CGCCGCTAGACCTTTTTGGA+TGG - chr4_2:55173972-55173991 None:intergenic 55.0%
TCAGGTTTACCTGGCCCAAC+TGG + chr4_2:55174322-55174341 Msa0608140:intron 55.0%
GAAGTGGAGGTGGTTGAAGG+GGG + chr4_2:55174391-55174410 Msa0608140:CDS 55.0%
GTCCCTTGAACTCCGTGAAG+CGG + chr4_2:55174482-55174501 Msa0608140:CDS 55.0%
CTAGGTCAACGCCTCAACGG+AGG - chr4_2:55173679-55173698 None:intergenic 60.0%
!! CCGTTGAGGCGTTGACCTAG+TGG + chr4_2:55173679-55173698 Msa0608140:intron 60.0%
! GCTGCGCCGCTAGACCTTTT+TGG - chr4_2:55173976-55173995 None:intergenic 60.0%
GGCCGCTTCACGGAGTTCAA+GGG - chr4_2:55174487-55174506 None:intergenic 60.0%
TGGCCGCTTCACGGAGTTCA+AGG - chr4_2:55174488-55174507 None:intergenic 60.0%
CTAGGTCAACGCCTCAACGG+AGG - chr4_2:55173679-55173698 None:intergenic 60.0%
!! CCGTTGAGGCGTTGACCTAG+TGG + chr4_2:55173679-55173698 Msa0608140:intron 60.0%
! GCTGCGCCGCTAGACCTTTT+TGG - chr4_2:55173976-55173995 None:intergenic 60.0%
GGCCGCTTCACGGAGTTCAA+GGG - chr4_2:55174487-55174506 None:intergenic 60.0%
TGGCCGCTTCACGGAGTTCA+AGG - chr4_2:55174488-55174507 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 55173161 55174833 55173161 ID=Msa0608140;Name=Msa0608140
chr4_2 mRNA 55173161 55174833 55173161 ID=Msa0608140-mRNA-1;Parent=Msa0608140;Name=Msa0608140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=203|1|1|1|1|1|2|308|127
chr4_2 exon 55173161 55173547 55173161 ID=Msa0608140-mRNA-1:exon:13968;Parent=Msa0608140-mRNA-1
chr4_2 exon 55174326 55174833 55174326 ID=Msa0608140-mRNA-1:exon:13969;Parent=Msa0608140-mRNA-1
chr4_2 five_prime_UTR 55173161 55173363 55173161 ID=Msa0608140-mRNA-1:five_prime_utr;Parent=Msa0608140-mRNA-1
chr4_2 CDS 55173364 55173547 55173364 ID=Msa0608140-mRNA-1:cds;Parent=Msa0608140-mRNA-1
chr4_2 CDS 55174326 55174525 55174326 ID=Msa0608140-mRNA-1:cds;Parent=Msa0608140-mRNA-1
chr4_2 three_prime_UTR 55174526 55174833 55174526 ID=Msa0608140-mRNA-1:three_prime_utr;Parent=Msa0608140-mRNA-1
Gene Sequence

>Msa0608140

ATGTTTGGTCAACTTGAGCATGAGTTAGAGCTGCACGTGCCAGCAAGTGAAGCATGGGATCTCTTTGGCACTCTTGCTATTGGAAAGGTCGTTGAGGAAGCGATGCCTGAAAGATTTCAGAAGGTGGAGCTAATTGAAGGAGATGGAGGGGTTGGGACTATTCTCAAACTCACATTCACTCCAGGTTTACCTGGCCCAACTGGTTTAAAAGAAAAGTTTACCAAGATTGATAATGAAAAGCACATCAAAGAAGTGGAGGTGGTTGAAGGGGGATGCTTAGATCTTGGATTCACACTATTTAGAGTTCGTATGGAAGTGATAGAGAAAGGTAAAGATATATGTCCCTTGAACTCCGTGAAGCGGCCAAACTTCATTCTCACATAG
Protein sequence

>Msa0608140

MFGQLEHELELHVPASEAWDLFGTLAIGKVVEEAMPERFQKVELIEGDGGVGTILKLTFTPGLPGPTGLKEKFTKIDNEKHIKEVEVVEGGCLDLGFTLFRVRMEVIEKGKDICPLNSVKRPNFILT*