AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1047440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1047440 MtrunA17_Chr7g0215471 90.323 93 9 0 1 93 5 97 3.57e-57 171
Msa1047440 MtrunA17_Chr7g0215501 81.429 70 13 0 1 70 1 70 1.00e-33 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1047440 AT1G47820.1 55.714 70 30 1 1 69 1 70 3.70e-21 80.5
Msa1047440 AT1G47820.2 55.714 70 30 1 1 69 1 70 3.70e-21 80.5

Find 24 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTTTATTGAAGAATTTA+AGG 0.202532 7_2:-8314403 Msa1047440:three_prime_UTR
AGAATTTAAGGAATATTATT+TGG 0.230448 7_2:-8314391 Msa1047440:three_prime_UTR
CATACCAATATTTAACTTCA+TGG 0.252212 7_2:-8314356 Msa1047440:three_prime_UTR
GCTCAAACACGACAGGATTT+AGG 0.308005 7_2:+8317135 None:intergenic
ATTTCCATGAAGTTAAATAT+TGG 0.313903 7_2:+8314352 None:intergenic
CACAGAGTCATATTCTGTTC+AGG 0.414511 7_2:-8314691 Msa1047440:CDS
GTGGCATTCACCTGAGGTCT+AGG 0.432602 7_2:+8314726 None:intergenic
ACAAAGCATGGCCGTATTGA+AGG 0.443308 7_2:-8314622 Msa1047440:CDS
TGTTATGAAATCCTTCAATA+CGG 0.467267 7_2:+8314611 None:intergenic
TGACAATGATGTTGCTAAGA+AGG 0.469068 7_2:-8314532 Msa1047440:CDS
TATTCTGTTCAGGCAATCAA+TGG 0.473465 7_2:-8314681 Msa1047440:CDS
TGCAGTGCTTCCTAGACCTC+AGG 0.506425 7_2:-8314736 Msa1047440:CDS
ATTTAAGGAATATTATTTGG+TGG 0.519709 7_2:-8314388 Msa1047440:three_prime_UTR
TGCTAAGAAGGCTCAAGTAA+AGG 0.526582 7_2:-8314520 Msa1047440:CDS
GAAGCTTCTAACGACTCGTC+TGG 0.528210 7_2:+8314564 None:intergenic
CGAGTCGTTAGAAGCTTCCT+CGG 0.555746 7_2:-8314559 Msa1047440:CDS
GACTCGTCTGGAACATCTTG+AGG 0.569302 7_2:+8314576 None:intergenic
TGAAGCAGCTCAAACACGAC+AGG 0.596549 7_2:+8317128 None:intergenic
TAACATGAAAGAACAAAGCA+TGG 0.616394 7_2:-8314634 Msa1047440:CDS
GTGAATGCCACATTTCGTCT+CGG 0.619096 7_2:-8314714 Msa1047440:CDS
AACATCATTGTCATCTTCCG+AGG 0.626572 7_2:+8314542 None:intergenic
CGAAATGTGGCATTCACCTG+AGG 0.643629 7_2:+8314720 None:intergenic
CTCTGTGCCGAGACGAAATG+TGG 0.646370 7_2:+8314707 None:intergenic
ATATGTCTAAAATGTATGAG+TGG 0.669924 7_2:-8314466 Msa1047440:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATACTATTCTAAATA+AGG + chr7_2:8315857-8315876 None:intergenic 10.0%
!! AAAAAAAAAAAAAAACATGA+AGG + chr7_2:8317089-8317108 None:intergenic 10.0%
!! ATAAACTGAAAATAGTTTAT+GGG + chr7_2:8314675-8314694 None:intergenic 15.0%
!! AATAAACTGAAAATAGTTTA+TGG + chr7_2:8314676-8314695 None:intergenic 15.0%
!!! TATTTTCAGTTTATTTTCAT+AGG - chr7_2:8314681-8314700 Msa1047440:CDS 15.0%
!! TAGATAAATAGTTAATTCTT+TGG - chr7_2:8314906-8314925 Msa1047440:intron 15.0%
!!! ACTTATAACTAATATTTTCT+TGG - chr7_2:8315896-8315915 Msa1047440:intron 15.0%
!!! CTTATAACTAATATTTTCTT+GGG - chr7_2:8315897-8315916 Msa1047440:intron 15.0%
!!! TGTTGATGAAAAATTTTATA+GGG + chr7_2:8316000-8316019 None:intergenic 15.0%
!!! ATGTTGATGAAAAATTTTAT+AGG + chr7_2:8316001-8316020 None:intergenic 15.0%
!! ATATTTCAAAATATATCCAA+AGG + chr7_2:8316498-8316517 None:intergenic 15.0%
!! TTAGTTTATTGAAGAATTTA+AGG - chr7_2:8317003-8317022 Msa1047440:intron 15.0%
!! AGAATTTAAGGAATATTATT+TGG - chr7_2:8317015-8317034 Msa1047440:intron 15.0%
!! AAAAAGAATCATTAAAACTA+TGG + chr7_2:8317135-8317154 None:intergenic 15.0%
!! AATGTAGTTTATGAAAAAAC+AGG - chr7_2:8315042-8315061 Msa1047440:intron 20.0%
!! ATGTAGTTTATGAAAAAACA+GGG - chr7_2:8315043-8315062 Msa1047440:intron 20.0%
!!! TTTGAGTAAATTTTATCTAC+TGG - chr7_2:8315175-8315194 Msa1047440:intron 20.0%
!!! ACATGATTTCGATTTTTATA+AGG - chr7_2:8315739-8315758 Msa1047440:intron 20.0%
!!! TTAGAATAGTATTTTTTGAG+AGG - chr7_2:8315860-8315879 Msa1047440:intron 20.0%
!! CAAAATTCTGAATATTCATA+GGG + chr7_2:8315940-8315959 None:intergenic 20.0%
!! ACAAAATTCTGAATATTCAT+AGG + chr7_2:8315941-8315960 None:intergenic 20.0%
!!! AAGTGTGATTTTATTTTGTA+GGG + chr7_2:8316130-8316149 None:intergenic 20.0%
!!! AAAGTGTGATTTTATTTTGT+AGG + chr7_2:8316129-8316148 None:intergenic 20.0%
!!! AAATTTCCATCAACATTTTT+TGG - chr7_2:8316069-8316088 Msa1047440:intron 20.0%
!!! AAGTGTGATTTTATTTTGTA+TGG - chr7_2:8316130-8316149 Msa1047440:intron 20.0%
!! ACAAAACTGTGAATATTTAT+AGG - chr7_2:8316160-8316179 Msa1047440:intron 20.0%
!! CAAAACTGTGAATATTTATA+GGG - chr7_2:8316161-8316180 Msa1047440:intron 20.0%
!!! TTAATTAACCCTATTTTCTT+TGG - chr7_2:8316193-8316212 Msa1047440:intron 20.0%
!! AATATTATAAAGAGATGAGA+AGG - chr7_2:8316222-8316241 Msa1047440:intron 20.0%
!! ATTTAAGGAATATTATTTGG+TGG - chr7_2:8317018-8317037 Msa1047440:intron 20.0%
!! ATTTCCATGAAGTTAAATAT+TGG + chr7_2:8317057-8317076 None:intergenic 20.0%
!! AAGAAAATCGAAATGTTTTG+CGG + chr7_2:8314375-8314394 None:intergenic 25.0%
! TATGTTAACTGTTTGATATG+AGG - chr7_2:8314809-8314828 Msa1047440:intron 25.0%
! AATAGTTAATTCTTTGGACT+CGG - chr7_2:8314912-8314931 Msa1047440:intron 25.0%
! AATAAAGAAACGAAGAAACT+AGG + chr7_2:8315450-8315469 None:intergenic 25.0%
! TAGTTTCTTCGTTTCTTTAT+TGG - chr7_2:8315449-8315468 Msa1047440:intron 25.0%
! TGAAATACTTAGACTTTGTT+TGG - chr7_2:8315774-8315793 Msa1047440:intron 25.0%
!! GAAATACTTAGACTTTGTTT+GGG - chr7_2:8315775-8315794 Msa1047440:intron 25.0%
!!! AATAGTATTTTTTGAGAGGA+TGG - chr7_2:8315864-8315883 Msa1047440:intron 25.0%
!! GCTGATGGAAAATTTTTATA+GGG + chr7_2:8316031-8316050 None:intergenic 25.0%
!! TGCTGATGGAAAATTTTTAT+AGG + chr7_2:8316032-8316051 None:intergenic 25.0%
!! CATTTTCCTTAAGCATTTTA+GGG + chr7_2:8316096-8316115 None:intergenic 25.0%
!! ACATTTTCCTTAAGCATTTT+AGG + chr7_2:8316097-8316116 None:intergenic 25.0%
! TTCTCAAACCAAAGAAAATA+GGG + chr7_2:8316204-8316223 None:intergenic 25.0%
! ATTCTCAAACCAAAGAAAAT+AGG + chr7_2:8316205-8316224 None:intergenic 25.0%
! TATGAGACTAAGAACAAATA+CGG - chr7_2:8316448-8316467 Msa1047440:intron 25.0%
! TTTGAAAATACTGTTTCCTT+TGG - chr7_2:8316479-8316498 Msa1047440:intron 25.0%
!!! TTTAAAAGTAGCACTGATTA+CGG + chr7_2:8316577-8316596 None:intergenic 25.0%
! AATTATTCAAAATGCAGAGA+TGG - chr7_2:8316631-8316650 Msa1047440:intron 25.0%
! TGTTATGAAATCCTTCAATA+CGG + chr7_2:8316798-8316817 None:intergenic 25.0%
!! ATATGTCTAAAATGTATGAG+TGG - chr7_2:8316940-8316959 Msa1047440:intron 25.0%
!! CATACCAATATTTAACTTCA+TGG - chr7_2:8317050-8317069 Msa1047440:intron 25.0%
!! CAGTTTATTTTCATAGGTCT+TGG - chr7_2:8314687-8314706 Msa1047440:CDS 30.0%
! CTTTCAATTTTGTATGCTTG+AGG - chr7_2:8314844-8314863 Msa1047440:intron 30.0%
ATAGTTAATTCTTTGGACTC+GGG - chr7_2:8314913-8314932 Msa1047440:intron 30.0%
CAAAATAAGCCTAATCCAAA+CGG - chr7_2:8315149-8315168 Msa1047440:intron 30.0%
AAAATAAGCCTAATCCAAAC+GGG - chr7_2:8315150-8315169 Msa1047440:intron 30.0%
TAAACATTTGTGAGACTGTT+TGG - chr7_2:8315200-8315219 Msa1047440:intron 30.0%
AAACATTTGTGAGACTGTTT+GGG - chr7_2:8315201-8315220 Msa1047440:intron 30.0%
! GGAAATTTTCCATAAACAGA+TGG + chr7_2:8315256-8315275 None:intergenic 30.0%
AAGCTATTGTTGAGAGTTTA+TGG + chr7_2:8315277-8315296 None:intergenic 30.0%
TAAACTGTTTACATCCAAAC+AGG - chr7_2:8315405-8315424 Msa1047440:intron 30.0%
AAACTGTTTACATCCAAACA+GGG - chr7_2:8315406-8315425 Msa1047440:intron 30.0%
! GCTTTTCAACTAACGAAAAA+AGG + chr7_2:8315679-8315698 None:intergenic 30.0%
TGTTCAACAATAGCACAATA+TGG + chr7_2:8315722-8315741 None:intergenic 30.0%
AAGTCTAAGTATTTCACTTG+AGG + chr7_2:8315770-8315789 None:intergenic 30.0%
!! GTTGATGGAAATTTTTGACA+GGG + chr7_2:8316063-8316082 None:intergenic 30.0%
!! TGTTGATGGAAATTTTTGAC+AGG + chr7_2:8316064-8316083 None:intergenic 30.0%
TTCATTGAACCAAAACTCTT+CGG + chr7_2:8316312-8316331 None:intergenic 30.0%
AGAATATACTGACGATAAAC+GGG + chr7_2:8316378-8316397 None:intergenic 30.0%
GAGAATATACTGACGATAAA+CGG + chr7_2:8316379-8316398 None:intergenic 30.0%
TAACATGAAAGAACAAAGCA+TGG - chr7_2:8316772-8316791 Msa1047440:intron 30.0%
CTGAAAATAGTTTATGGGCA+TGG + chr7_2:8314670-8314689 None:intergenic 35.0%
GTTGAGCTTATCTTGACATT+TGG - chr7_2:8314870-8314889 Msa1047440:intron 35.0%
TAGTTAATTCTTTGGACTCG+GGG - chr7_2:8314914-8314933 Msa1047440:intron 35.0%
AATTTACTCAAATGCCCGTT+TGG + chr7_2:8315167-8315186 None:intergenic 35.0%
ACGCTTATAGCATAAGCTAT+AGG + chr7_2:8315380-8315399 None:intergenic 35.0%
CTAGGAAATGAAGGAAACTA+AGG + chr7_2:8315432-8315451 None:intergenic 35.0%
ACGAAGAAACTAGGAAATGA+AGG + chr7_2:8315441-8315460 None:intergenic 35.0%
! TCTTCTATCTGATCTAGTTC+TGG - chr7_2:8315518-8315537 Msa1047440:intron 35.0%
TTCAGCTATTGCATTTCAGA+AGG + chr7_2:8315585-8315604 None:intergenic 35.0%
CAACAATAGCACAATATGGA+TGG + chr7_2:8315718-8315737 None:intergenic 35.0%
! TTAGACTTTGTTTGGGAGTT+AGG - chr7_2:8315782-8315801 Msa1047440:intron 35.0%
TAGGGACCAAAAAATGTTGA+TGG + chr7_2:8316078-8316097 None:intergenic 35.0%
TTTGGTCCCTAAAATGCTTA+AGG - chr7_2:8316087-8316106 Msa1047440:intron 35.0%
!! TAAAGCTTTGTTTGCGAGTT+TGG + chr7_2:8316425-8316444 None:intergenic 35.0%
CAAAGACAACAGAACAAACT+GGG + chr7_2:8316534-8316553 None:intergenic 35.0%
TCAAAGACAACAGAACAAAC+TGG + chr7_2:8316535-8316554 None:intergenic 35.0%
TATTCTGTTCAGGCAATCAA+TGG - chr7_2:8316725-8316744 Msa1047440:intron 35.0%
TGACAATGATGTTGCTAAGA+AGG - chr7_2:8316874-8316893 Msa1047440:intron 35.0%
TCTCTCTCTCTCTCTCAATA+AGG - chr7_2:8314345-8314364 Msa1047440:three_prime_UTR 40.0%
GTTCTGCTTGAGGAATTGTT+AGG - chr7_2:8314558-8314577 Msa1047440:CDS 40.0%
! GGGTCTGTTTGATTGACTTA+GGG - chr7_2:8314934-8314953 Msa1047440:intron 40.0%
GTGACATGTCCATCTGTTTA+TGG - chr7_2:8315244-8315263 Msa1047440:intron 40.0%
TGGATGGCTTTCAAACTCAT+GGG + chr7_2:8315702-8315721 None:intergenic 40.0%
ATGGATGGCTTTCAAACTCA+TGG + chr7_2:8315703-8315722 None:intergenic 40.0%
TTTGTTTGGGAGTTAGGATG+AGG - chr7_2:8315788-8315807 Msa1047440:intron 40.0%
AGGAAATAGGAGGAAGGAAA+AGG - chr7_2:8315817-8315836 Msa1047440:intron 40.0%
ACAGGGACTAAAAATGCTGA+TGG + chr7_2:8316046-8316065 None:intergenic 40.0%
TGTCGAGGAAAGACTTTGAA+GGG + chr7_2:8316268-8316287 None:intergenic 40.0%
CTAACTCAAGAAATGTGTCG+AGG + chr7_2:8316283-8316302 None:intergenic 40.0%
! TAGTCCTTACCGAAGAGTTT+TGG - chr7_2:8316300-8316319 Msa1047440:intron 40.0%
TGAACCAAAACTCTTCGGTA+AGG + chr7_2:8316307-8316326 None:intergenic 40.0%
GAAAAGTAAACCTCCATGCA+AGG - chr7_2:8316332-8316351 Msa1047440:intron 40.0%
TACTGACGATAAACGGGAAA+GGG + chr7_2:8316372-8316391 None:intergenic 40.0%
ATACTGACGATAAACGGGAA+AGG + chr7_2:8316373-8316392 None:intergenic 40.0%
GAGACTAAGAACAAATACGG+TGG - chr7_2:8316451-8316470 Msa1047440:intron 40.0%
CAACAGAACAAACTGGGTAT+TGG + chr7_2:8316528-8316547 None:intergenic 40.0%
CACAGAGTCATATTCTGTTC+AGG - chr7_2:8316715-8316734 Msa1047440:intron 40.0%
AACATCATTGTCATCTTCCG+AGG + chr7_2:8316867-8316886 None:intergenic 40.0%
TGCTAAGAAGGCTCAAGTAA+AGG - chr7_2:8316886-8316905 Msa1047440:intron 40.0%
! GGGGTCTGTTTGATTGACTT+AGG - chr7_2:8314933-8314952 Msa1047440:intron 45.0%
! TGATTGACTTAGGGCACGTT+TGG - chr7_2:8314943-8314962 Msa1047440:intron 45.0%
ACTTAGGGCACGTTTGGATT+AGG - chr7_2:8314949-8314968 Msa1047440:intron 45.0%
CTCAAATGCCCGTTTGGATT+AGG + chr7_2:8315161-8315180 None:intergenic 45.0%
AAGGAAACTAAGGCCCTGTT+TGG + chr7_2:8315422-8315441 None:intergenic 45.0%
GGATGGCTTTCAAACTCATG+GGG + chr7_2:8315701-8315720 None:intergenic 45.0%
GTGTCGAGGAAAGACTTTGA+AGG + chr7_2:8316269-8316288 None:intergenic 45.0%
AAGTAAACCTCCATGCAAGG+TGG - chr7_2:8316335-8316354 Msa1047440:intron 45.0%
AGTAAACCTCCATGCAAGGT+GGG - chr7_2:8316336-8316355 Msa1047440:intron 45.0%
ACTGACGATAAACGGGAAAG+GGG + chr7_2:8316371-8316390 None:intergenic 45.0%
!! AGCTTTGTTTGCGAGTTTGG+AGG + chr7_2:8316422-8316441 None:intergenic 45.0%
GTGAATGCCACATTTCGTCT+CGG - chr7_2:8316692-8316711 Msa1047440:intron 45.0%
ACAAAGCATGGCCGTATTGA+AGG - chr7_2:8316784-8316803 Msa1047440:intron 45.0%
TGAAGCAGCTCAAACACGAC+AGG + chr7_2:8314281-8314300 None:intergenic 50.0%
GTCTGTGTGTGTTCTGCTTG+AGG - chr7_2:8314548-8314567 Msa1047440:CDS 50.0%
GATGAGGCCTTGGAGGAAAT+AGG - chr7_2:8315804-8315823 Msa1047440:intron 50.0%
CCTTCCTCCTATTTCCTCCA+AGG + chr7_2:8315814-8315833 None:intergenic 50.0%
CCTTGGAGGAAATAGGAGGA+AGG - chr7_2:8315811-8315830 Msa1047440:intron 50.0%
GTAAACCTCCATGCAAGGTG+GGG - chr7_2:8316337-8316356 Msa1047440:intron 50.0%
TAAACCTCCATGCAAGGTGG+GGG - chr7_2:8316338-8316357 Msa1047440:intron 50.0%
AAATAGTCCCCCACCTTGCA+TGG + chr7_2:8316348-8316367 None:intergenic 50.0%
GAGTTTGGAGGAGAAGTGAG+GGG + chr7_2:8316410-8316429 None:intergenic 50.0%
CGAGTTTGGAGGAGAAGTGA+GGG + chr7_2:8316411-8316430 None:intergenic 50.0%
CGAAATGTGGCATTCACCTG+AGG + chr7_2:8316689-8316708 None:intergenic 50.0%
! GACTCGTCTGGAACATCTTG+AGG + chr7_2:8316833-8316852 None:intergenic 50.0%
GAAGCTTCTAACGACTCGTC+TGG + chr7_2:8316845-8316864 None:intergenic 50.0%
CGAGTCGTTAGAAGCTTCCT+CGG - chr7_2:8316847-8316866 Msa1047440:intron 50.0%
TGGGAGTTAGGATGAGGCCT+TGG - chr7_2:8315794-8315813 Msa1047440:intron 55.0%
GAGTTAGGATGAGGCCTTGG+AGG - chr7_2:8315797-8315816 Msa1047440:intron 55.0%
GAGGCCTTGGAGGAAATAGG+AGG - chr7_2:8315807-8315826 Msa1047440:intron 55.0%
GGAGAAGTGAGGGGAAGAGT+TGG + chr7_2:8316401-8316420 None:intergenic 55.0%
GCGAGTTTGGAGGAGAAGTG+AGG + chr7_2:8316412-8316431 None:intergenic 55.0%
TGCAGTGCTTCCTAGACCTC+AGG - chr7_2:8316670-8316689 Msa1047440:intron 55.0%
GTGGCATTCACCTGAGGTCT+AGG + chr7_2:8316683-8316702 None:intergenic 55.0%
CTCTGTGCCGAGACGAAATG+TGG + chr7_2:8316702-8316721 None:intergenic 55.0%
TAGTCCCCCACCTTGCATGG+AGG + chr7_2:8316345-8316364 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 8314272 8317156 8314272 ID=Msa1047440;Name=Msa1047440
chr7_2 mRNA 8314272 8317156 8314272 ID=Msa1047440-mRNA-1;Parent=Msa1047440;Name=Msa1047440-mRNA-1;_AED=0.16;_eAED=0.16;_QI=95|0|0.5|1|0|0.5|2|225|93
chr7_2 exon 8314272 8314778 8314272 ID=Msa1047440-mRNA-1:exon:2199;Parent=Msa1047440-mRNA-1
chr7_2 exon 8317062 8317156 8317062 ID=Msa1047440-mRNA-1:exon:2198;Parent=Msa1047440-mRNA-1
chr7_2 five_prime_UTR 8317062 8317156 8317062 ID=Msa1047440-mRNA-1:five_prime_utr;Parent=Msa1047440-mRNA-1
chr7_2 CDS 8314497 8314778 8314497 ID=Msa1047440-mRNA-1:cds;Parent=Msa1047440-mRNA-1
chr7_2 three_prime_UTR 8314272 8314496 8314272 ID=Msa1047440-mRNA-1:three_prime_utr;Parent=Msa1047440-mRNA-1
Gene Sequence

>Msa1047440

ATGGAAATAGAATCAAGTGATGCAGTGCTTCCTAGACCTCAGGTGAATGCCACATTTCGTCTCGGCACAGAGTCATATTCTGTTCAGGCAATCAATGGTTCTTCATTATCAGAGCAACTCGTTAACATGAAAGAACAAAGCATGGCCGTATTGAAGGATTTCATAACAAAGCATAATATTCCTCAAGATGTTCCAGACGAGTCGTTAGAAGCTTCCTCGGAAGATGACAATGATGTTGCTAAGAAGGCTCAAGTAAAGGCTAAGAAAACTAAGCTTACTTAG

Protein sequence

>Msa1047440

MEIESSDAVLPRPQVNATFRLGTESYSVQAINGSSLSEQLVNMKEQSMAVLKDFITKHNIPQDVPDESLEASSEDDNDVAKKAQVKAKKTKLT*