AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0946210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0946210 MtrunA17_Chr6g0459281 88.462 78 9 0 1 78 1 78 1.67e-43 141
Msa0946210 MtrunA17_Chr6g0459351 78.205 78 17 0 1 78 1 78 8.37e-39 129
Msa0946210 MtrunA17_Chr6g0459331 71.795 78 22 0 1 78 1 78 4.08e-34 117
Msa0946210 MtrunA17_Chr6g0459271 71.795 78 22 0 1 78 1 78 7.95e-34 116
Msa0946210 MtrunA17_Chr6g0459261 69.231 78 24 0 1 78 13 90 2.19e-33 115
Msa0946210 MtrunA17_Chr6g0459301 68.354 79 24 1 1 78 1 79 7.63e-31 108
Msa0946210 MtrunA17_Chr6g0459341 51.852 108 28 2 1 86 1 106 3.27e-25 90.1
Msa0946210 MtrunA17_Chr7g0266981 57.692 78 32 1 1 78 1 77 4.48e-24 91.7
Msa0946210 MtrunA17_Chr4g0044111 57.692 78 32 1 1 78 1 77 4.72e-24 91.7
Msa0946210 MtrunA17_Chr7g0270461 62.687 67 24 1 12 78 45 110 3.37e-21 81.3
Msa0946210 MtrunA17_Chr7g0270451 55.000 80 29 2 12 85 5 83 2.24e-20 81.6
Msa0946210 MtrunA17_Chr7g0266891 59.091 66 26 1 13 78 7 71 2.98e-20 81.3
Msa0946210 MtrunA17_Chr7g0270441 64.179 67 23 1 12 78 5 70 2.99e-20 81.3
Msa0946210 MtrunA17_Chr7g0267011 50.725 69 33 1 10 78 5 72 6.08e-18 73.9
Msa0946210 MtrunA17_Chr2g0324021 58.209 67 27 1 12 78 5 70 1.13e-17 74.7
Msa0946210 MtrunA17_Chr4g0026001 50.746 67 32 1 12 78 13 78 7.82e-17 72.4
Msa0946210 MtrunA17_Chr4g0044131 53.731 67 30 1 12 78 7 72 1.09e-16 72.0
Msa0946210 MtrunA17_Chr7g0267001 52.239 67 31 1 12 78 7 72 1.27e-16 71.6
Msa0946210 MtrunA17_Chr4g0025971 46.835 79 40 2 1 78 1 78 1.65e-16 71.6
Msa0946210 MtrunA17_Chr4g0040371 56.716 67 25 2 12 78 18 80 3.19e-16 70.9
Msa0946210 MtrunA17_Chr4g0025981 50.000 64 31 1 15 78 43 105 3.68e-15 68.2
Msa0946210 MtrunA17_Chr3g0084871 50.000 74 35 2 3 75 6 78 7.46e-15 67.0
Msa0946210 MtrunA17_Chr7g0268591 48.649 74 36 2 6 79 26 97 7.70e-15 67.4
Msa0946210 MtrunA17_Chr7g0252031 47.945 73 36 2 6 78 28 98 8.51e-15 67.0
Msa0946210 MtrunA17_Chr7g0266991 65.306 49 16 1 12 60 24 71 1.46e-13 63.2
Msa0946210 MtrunA17_Chr7g0244061 47.761 67 33 2 12 78 28 92 1.48e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0946210 AT2G47140.1 57.143 70 29 1 9 78 2 70 1.05e-21 85.5
Msa0946210 AT2G47130.1 54.286 70 31 1 9 78 2 70 2.96e-20 81.6
Msa0946210 AT2G47120.1 52.857 70 32 1 9 78 2 70 3.91e-19 78.6
Msa0946210 AT3G29250.3 52.055 73 34 1 3 75 32 103 5.41e-19 79.0
Msa0946210 AT3G29260.1 54.286 70 31 1 9 78 2 70 1.29e-18 77.4
Msa0946210 AT3G29250.2 55.224 67 29 1 9 75 2 67 2.14e-18 76.6
Msa0946210 AT2G47120.2 52.239 67 31 1 12 78 6 71 1.34e-17 74.7
Msa0946210 AT3G29250.1 50.667 75 34 2 3 75 32 105 4.05e-17 73.9
Msa0946210 AT3G51680.1 48.101 79 36 2 1 75 20 97 5.20e-14 65.5
Msa0946210 AT3G26760.1 50.746 67 31 2 12 78 35 99 1.54e-13 63.9
Msa0946210 AT3G42960.1 52.941 68 28 2 8 75 3 66 1.02e-12 61.6
Msa0946210 AT3G26770.1 50.000 64 30 2 12 75 40 101 1.52e-11 58.5
Msa0946210 AT1G52340.1 47.222 72 33 3 12 79 17 87 2.24e-11 58.2
Msa0946210 AT4G03140.1 46.269 67 34 2 12 78 77 141 5.38e-11 57.0

Find 27 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTACTAACAACGGTCTT+AGG 0.262828 6_3:+26634032 Msa0946210:CDS
CACCATTCGTGCACCTTGTT+CGG 0.349762 6_3:-26634125 None:intergenic
GCCAATGCCGCTGGCACCTC+CGG 0.364087 6_3:-26634080 None:intergenic
CGTCTCTTTGCCAATGCCGC+TGG 0.394229 6_3:-26634089 None:intergenic
GAGCGAGTGGCTGCATCCAT+TGG 0.421907 6_3:+26634178 Msa0946210:CDS
ACCGGAGGTGCCAGCGGCAT+TGG 0.425693 6_3:+26634079 Msa0946210:CDS
CTGCATCCATTGGCGGCCAC+AGG 0.444834 6_3:+26634188 Msa0946210:CDS
TGCTGACATTCAAGACGAGT+TGG 0.475276 6_3:+26634153 Msa0946210:CDS
GTGTTTGCAGTTAACATTCG+AGG 0.478434 6_3:+26635025 Msa0946210:intron
GCTGACATTCAAGACGAGTT+GGG 0.485584 6_3:+26634154 Msa0946210:CDS
GGTGACGATGGCTACTTTGC+CGG 0.490347 6_3:-26634059 None:intergenic
GGTGCTCCTGTGGCCGCCAA+TGG 0.498816 6_3:-26634194 None:intergenic
AACAACGGTCTTAGGTTAGC+CGG 0.520911 6_3:+26634040 Msa0946210:CDS
GGCAAAGTAGCCATCGTCAC+CGG 0.538495 6_3:+26634061 Msa0946210:CDS
AGACGAGTTGGGCGAGCGAG+TGG 0.553364 6_3:+26634165 Msa0946210:CDS
GCACATCTCTTTGCCGAACA+AGG 0.555212 6_3:+26634112 Msa0946210:CDS
TGCCGAACAAGGTGCACGAA+TGG 0.557302 6_3:+26634123 Msa0946210:CDS
CGAGTGGCTGCATCCATTGG+CGG 0.561662 6_3:+26634181 Msa0946210:CDS
CAGCGGCATTGGCAAAGAGA+CGG 0.569942 6_3:+26634090 Msa0946210:CDS
CGAACAAGGTGCACGAATGG+TGG 0.575204 6_3:+26634126 Msa0946210:CDS
GCTGGCACCTCCGGTGACGA+TGG 0.584463 6_3:-26634071 None:intergenic
ATCGTCACCGGAGGTGCCAG+CGG 0.590387 6_3:+26634073 Msa0946210:CDS
TGCTATGTCACAATGGATGA+AGG 0.590504 6_3:-26634215 None:intergenic
CTTCATTTGCTATGTCACAA+TGG 0.633784 6_3:-26634222 None:intergenic
AATGGATGAAGGTGCTCCTG+TGG 0.649213 6_3:-26634204 None:intergenic
AAAGTAGCCATCGTCACCGG+AGG 0.651370 6_3:+26634064 Msa0946210:CDS
GAAGCTTCATCTACTAACAA+CGG 0.689074 6_3:+26634025 Msa0946210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAATAAATTTTTTTTAT+AGG + chr6_3:26634752-26634771 Msa0946210:intron 0.0%
!!! TAAAATAAATTTTTTTTATA+GGG + chr6_3:26634753-26634772 Msa0946210:intron 0.0%
!!! ATAAAATAAATTTTTTTTAT+AGG + chr6_3:26634752-26634771 Msa0946210:intron 0.0%
!!! TAAAATAAATTTTTTTTATA+GGG + chr6_3:26634753-26634772 Msa0946210:intron 0.0%
!! AAAAAATCTCATTCAATTTA+CGG + chr6_3:26634252-26634271 Msa0946210:intron 15.0%
!! ACATTATAATGTAGAAATTA+TGG - chr6_3:26634604-26634623 None:intergenic 15.0%
!!! TAAAGTCTTATATAGTAAAT+GGG - chr6_3:26634797-26634816 None:intergenic 15.0%
!!! ATAAAGTCTTATATAGTAAA+TGG - chr6_3:26634798-26634817 None:intergenic 15.0%
!! AAAAAATCTCATTCAATTTA+CGG + chr6_3:26634252-26634271 Msa0946210:intron 15.0%
!! ACATTATAATGTAGAAATTA+TGG - chr6_3:26634604-26634623 None:intergenic 15.0%
!!! TAAAGTCTTATATAGTAAAT+GGG - chr6_3:26634797-26634816 None:intergenic 15.0%
!!! ATAAAGTCTTATATAGTAAA+TGG - chr6_3:26634798-26634817 None:intergenic 15.0%
!! CAAAATTTCATAATACACTT+AGG - chr6_3:26634330-26634349 None:intergenic 20.0%
!!! ATTATGGTTACTTTTATCTT+AGG - chr6_3:26634588-26634607 None:intergenic 20.0%
!!! TACTGATAGTATAATTTTGT+AGG + chr6_3:26634691-26634710 Msa0946210:intron 20.0%
!!! AAAGTCTTATATAGTAAATG+GGG - chr6_3:26634796-26634815 None:intergenic 20.0%
!!! GAAAGAAAAAGTTGTATTTT+AGG - chr6_3:26634908-26634927 None:intergenic 20.0%
!! CAAAATTTCATAATACACTT+AGG - chr6_3:26634330-26634349 None:intergenic 20.0%
!!! ATTATGGTTACTTTTATCTT+AGG - chr6_3:26634588-26634607 None:intergenic 20.0%
!!! TACTGATAGTATAATTTTGT+AGG + chr6_3:26634691-26634710 Msa0946210:intron 20.0%
!!! AAAGTCTTATATAGTAAATG+GGG - chr6_3:26634796-26634815 None:intergenic 20.0%
!!! GAAAGAAAAAGTTGTATTTT+AGG - chr6_3:26634908-26634927 None:intergenic 20.0%
!!! GAAGAATTTGAAGTTTTTTG+AGG - chr6_3:26634453-26634472 None:intergenic 25.0%
! ATATTATGTTTAGCAATGCT+GGG + chr6_3:26634945-26634964 Msa0946210:intron 25.0%
!!! GAAGAATTTGAAGTTTTTTG+AGG - chr6_3:26634453-26634472 None:intergenic 25.0%
! ATATTATGTTTAGCAATGCT+GGG + chr6_3:26634945-26634964 Msa0946210:intron 25.0%
TGGACAGGTAAAAATTAACT+AGG + chr6_3:26634285-26634304 Msa0946210:intron 30.0%
GGACAGGTAAAAATTAACTA+GGG + chr6_3:26634286-26634305 Msa0946210:intron 30.0%
! TAACTTTAATTAGTCTCTGC+TGG - chr6_3:26634673-26634692 None:intergenic 30.0%
! TTTTCTTTCTTTTCCACAAC+AGG + chr6_3:26634919-26634938 Msa0946210:intron 30.0%
AACATAATATCTACCTGTTG+TGG - chr6_3:26634935-26634954 None:intergenic 30.0%
GATATTATGTTTAGCAATGC+TGG + chr6_3:26634944-26634963 Msa0946210:intron 30.0%
TGGACAGGTAAAAATTAACT+AGG + chr6_3:26634285-26634304 Msa0946210:intron 30.0%
GGACAGGTAAAAATTAACTA+GGG + chr6_3:26634286-26634305 Msa0946210:intron 30.0%
! TAACTTTAATTAGTCTCTGC+TGG - chr6_3:26634673-26634692 None:intergenic 30.0%
! TTTTCTTTCTTTTCCACAAC+AGG + chr6_3:26634919-26634938 Msa0946210:intron 30.0%
AACATAATATCTACCTGTTG+TGG - chr6_3:26634935-26634954 None:intergenic 30.0%
GATATTATGTTTAGCAATGC+TGG + chr6_3:26634944-26634963 Msa0946210:intron 30.0%
GAAGCTTCATCTACTAACAA+CGG + chr6_3:26634025-26634044 Msa0946210:CDS 35.0%
!! CTTCATTTGCTATGTCACAA+TGG - chr6_3:26634225-26634244 None:intergenic 35.0%
CAATTTACGGTCAATGCTTA+TGG + chr6_3:26634265-26634284 Msa0946210:intron 35.0%
! TCCATCTGATCAGACTATTT+TGG + chr6_3:26634976-26634995 Msa0946210:intron 35.0%
TCCAAAATAGTCTGATCAGA+TGG - chr6_3:26634980-26634999 None:intergenic 35.0%
GAATGTTAACTGCAAACACA+TGG - chr6_3:26635024-26635043 None:intergenic 35.0%
GAAGCTTCATCTACTAACAA+CGG + chr6_3:26634025-26634044 Msa0946210:CDS 35.0%
!! CTTCATTTGCTATGTCACAA+TGG - chr6_3:26634225-26634244 None:intergenic 35.0%
CAATTTACGGTCAATGCTTA+TGG + chr6_3:26634265-26634284 Msa0946210:intron 35.0%
! TCCATCTGATCAGACTATTT+TGG + chr6_3:26634976-26634995 Msa0946210:intron 35.0%
TCCAAAATAGTCTGATCAGA+TGG - chr6_3:26634980-26634999 None:intergenic 35.0%
GAATGTTAACTGCAAACACA+TGG - chr6_3:26635024-26635043 None:intergenic 35.0%
CATCTACTAACAACGGTCTT+AGG + chr6_3:26634032-26634051 Msa0946210:CDS 40.0%
TGCTATGTCACAATGGATGA+AGG - chr6_3:26634218-26634237 None:intergenic 40.0%
GTTAACTGCAAACACATGGT+CGG - chr6_3:26635020-26635039 None:intergenic 40.0%
GTGTTTGCAGTTAACATTCG+AGG + chr6_3:26635025-26635044 Msa0946210:intron 40.0%
CATCTACTAACAACGGTCTT+AGG + chr6_3:26634032-26634051 Msa0946210:CDS 40.0%
TGCTATGTCACAATGGATGA+AGG - chr6_3:26634218-26634237 None:intergenic 40.0%
GTTAACTGCAAACACATGGT+CGG - chr6_3:26635020-26635039 None:intergenic 40.0%
GTGTTTGCAGTTAACATTCG+AGG + chr6_3:26635025-26635044 Msa0946210:intron 40.0%
AACAACGGTCTTAGGTTAGC+CGG + chr6_3:26634040-26634059 Msa0946210:CDS 45.0%
TGCTGACATTCAAGACGAGT+TGG + chr6_3:26634153-26634172 Msa0946210:CDS 45.0%
GCTGACATTCAAGACGAGTT+GGG + chr6_3:26634154-26634173 Msa0946210:CDS 45.0%
TACGGTCAATGCTTATGGAC+AGG + chr6_3:26634270-26634289 Msa0946210:intron 45.0%
AACAACGGTCTTAGGTTAGC+CGG + chr6_3:26634040-26634059 Msa0946210:CDS 45.0%
TGCTGACATTCAAGACGAGT+TGG + chr6_3:26634153-26634172 Msa0946210:CDS 45.0%
GCTGACATTCAAGACGAGTT+GGG + chr6_3:26634154-26634173 Msa0946210:CDS 45.0%
TACGGTCAATGCTTATGGAC+AGG + chr6_3:26634270-26634289 Msa0946210:intron 45.0%
GCACATCTCTTTGCCGAACA+AGG + chr6_3:26634112-26634131 Msa0946210:CDS 50.0%
CACCATTCGTGCACCTTGTT+CGG - chr6_3:26634128-26634147 None:intergenic 50.0%
!! AATGGATGAAGGTGCTCCTG+TGG - chr6_3:26634207-26634226 None:intergenic 50.0%
GCACATCTCTTTGCCGAACA+AGG + chr6_3:26634112-26634131 Msa0946210:CDS 50.0%
CACCATTCGTGCACCTTGTT+CGG - chr6_3:26634128-26634147 None:intergenic 50.0%
!! AATGGATGAAGGTGCTCCTG+TGG - chr6_3:26634207-26634226 None:intergenic 50.0%
! GGTGACGATGGCTACTTTGC+CGG - chr6_3:26634062-26634081 None:intergenic 55.0%
GGCAAAGTAGCCATCGTCAC+CGG + chr6_3:26634061-26634080 Msa0946210:CDS 55.0%
AAAGTAGCCATCGTCACCGG+AGG + chr6_3:26634064-26634083 Msa0946210:CDS 55.0%
!! CAGCGGCATTGGCAAAGAGA+CGG + chr6_3:26634090-26634109 Msa0946210:CDS 55.0%
TGCCGAACAAGGTGCACGAA+TGG + chr6_3:26634123-26634142 Msa0946210:CDS 55.0%
CGAACAAGGTGCACGAATGG+TGG + chr6_3:26634126-26634145 Msa0946210:CDS 55.0%
! GGTGACGATGGCTACTTTGC+CGG - chr6_3:26634062-26634081 None:intergenic 55.0%
GGCAAAGTAGCCATCGTCAC+CGG + chr6_3:26634061-26634080 Msa0946210:CDS 55.0%
AAAGTAGCCATCGTCACCGG+AGG + chr6_3:26634064-26634083 Msa0946210:CDS 55.0%
!! CAGCGGCATTGGCAAAGAGA+CGG + chr6_3:26634090-26634109 Msa0946210:CDS 55.0%
TGCCGAACAAGGTGCACGAA+TGG + chr6_3:26634123-26634142 Msa0946210:CDS 55.0%
CGAACAAGGTGCACGAATGG+TGG + chr6_3:26634126-26634145 Msa0946210:CDS 55.0%
CGTCTCTTTGCCAATGCCGC+TGG - chr6_3:26634092-26634111 None:intergenic 60.0%
GAGCGAGTGGCTGCATCCAT+TGG + chr6_3:26634178-26634197 Msa0946210:CDS 60.0%
CGAGTGGCTGCATCCATTGG+CGG + chr6_3:26634181-26634200 Msa0946210:CDS 60.0%
CGTCTCTTTGCCAATGCCGC+TGG - chr6_3:26634092-26634111 None:intergenic 60.0%
GAGCGAGTGGCTGCATCCAT+TGG + chr6_3:26634178-26634197 Msa0946210:CDS 60.0%
CGAGTGGCTGCATCCATTGG+CGG + chr6_3:26634181-26634200 Msa0946210:CDS 60.0%
!! ATCGTCACCGGAGGTGCCAG+CGG + chr6_3:26634073-26634092 Msa0946210:CDS 65.0%
! AGACGAGTTGGGCGAGCGAG+TGG + chr6_3:26634165-26634184 Msa0946210:CDS 65.0%
CTGCATCCATTGGCGGCCAC+AGG + chr6_3:26634188-26634207 Msa0946210:CDS 65.0%
!! ATCGTCACCGGAGGTGCCAG+CGG + chr6_3:26634073-26634092 Msa0946210:CDS 65.0%
! AGACGAGTTGGGCGAGCGAG+TGG + chr6_3:26634165-26634184 Msa0946210:CDS 65.0%
CTGCATCCATTGGCGGCCAC+AGG + chr6_3:26634188-26634207 Msa0946210:CDS 65.0%
GCTGGCACCTCCGGTGACGA+TGG - chr6_3:26634074-26634093 None:intergenic 70.0%
!! ACCGGAGGTGCCAGCGGCAT+TGG + chr6_3:26634079-26634098 Msa0946210:CDS 70.0%
GCCAATGCCGCTGGCACCTC+CGG - chr6_3:26634083-26634102 None:intergenic 70.0%
GGTGCTCCTGTGGCCGCCAA+TGG - chr6_3:26634197-26634216 None:intergenic 70.0%
GCTGGCACCTCCGGTGACGA+TGG - chr6_3:26634074-26634093 None:intergenic 70.0%
!! ACCGGAGGTGCCAGCGGCAT+TGG + chr6_3:26634079-26634098 Msa0946210:CDS 70.0%
GCCAATGCCGCTGGCACCTC+CGG - chr6_3:26634083-26634102 None:intergenic 70.0%
GGTGCTCCTGTGGCCGCCAA+TGG - chr6_3:26634197-26634216 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 26634019 26635064 26634019 ID=Msa0946210;Name=Msa0946210
chr6_3 mRNA 26634019 26635064 26634019 ID=Msa0946210-mRNA-1;Parent=Msa0946210;Name=Msa0946210-mRNA-1;_AED=0.45;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|86
chr6_3 exon 26634019 26634249 26634019 ID=Msa0946210-mRNA-1:exon:7371;Parent=Msa0946210-mRNA-1
chr6_3 exon 26635035 26635064 26635035 ID=Msa0946210-mRNA-1:exon:7372;Parent=Msa0946210-mRNA-1
chr6_3 CDS 26634019 26634249 26634019 ID=Msa0946210-mRNA-1:cds;Parent=Msa0946210-mRNA-1
chr6_3 CDS 26635035 26635064 26635035 ID=Msa0946210-mRNA-1:cds;Parent=Msa0946210-mRNA-1
Gene Sequence

>Msa0946210

ATGGCAGAAGCTTCATCTACTAACAACGGTCTTAGGTTAGCCGGCAAAGTAGCCATCGTCACCGGAGGTGCCAGCGGCATTGGCAAAGAGACGGCACATCTCTTTGCCGAACAAGGTGCACGAATGGTGGTTATTGCTGACATTCAAGACGAGTTGGGCGAGCGAGTGGCTGCATCCATTGGCGGCCACAGGAGCACCTTCATCCATTGTGACATAGCAAATGAAGATCAATTAACATTCGAGGAACGACATTGTGCGTGA

Protein sequence

>Msa0946210

MAEASSTNNGLRLAGKVAIVTGGASGIGKETAHLFAEQGARMVVIADIQDELGERVAASIGGHRSTFIHCDIANEDQLTFEERHCA*