Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1135380 | KEH21597.1 | 95.798 | 357 | 14 | 1 | 1 | 357 | 1 | 356 | 0.0 | 702 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1135380 | sp|Q8LB81|GDL79_ARATH | 66.859 | 347 | 101 | 2 | 23 | 357 | 22 | 366 | 9.66e-174 | 490 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1135380 | A0A072TVX2 | 95.798 | 357 | 14 | 1 | 1 | 357 | 1 | 356 | 0.0 | 702 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006980 | Msa1135380 | 0.800471 | 1.419330e-48 | -8.615850e-47 |
| Msa0074650 | Msa1135380 | 0.817091 | 3.946120e-52 | -8.615850e-47 |
| Msa0104500 | Msa1135380 | 0.821350 | 4.230015e-53 | -8.615850e-47 |
| Msa0124130 | Msa1135380 | 0.811359 | 7.281394e-51 | -8.615850e-47 |
| Msa0149140 | Msa1135380 | 0.832597 | 8.631923e-56 | -8.615850e-47 |
| Msa0194380 | Msa1135380 | 0.825907 | 3.628031e-54 | -8.615850e-47 |
| Msa0212150 | Msa1135380 | 0.835834 | 1.331956e-56 | -8.615850e-47 |
| Msa0252770 | Msa1135380 | 0.829687 | 4.476829e-55 | -8.615850e-47 |
| Msa0289920 | Msa1135380 | 0.821512 | 3.881941e-53 | -8.615850e-47 |
| Msa0306120 | Msa1135380 | 0.826426 | 2.730275e-54 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1135380 | MtrunA17_Chr7g0215961 | 95.798 | 357 | 14 | 1 | 1 | 357 | 1 | 356 | 0.0 | 702 |
| Msa1135380 | MtrunA17_Chr7g0275021 | 68.919 | 370 | 102 | 2 | 1 | 357 | 1 | 370 | 0.0 | 539 |
| Msa1135380 | MtrunA17_Chr6g0457921 | 67.116 | 371 | 103 | 4 | 1 | 357 | 1 | 366 | 0.0 | 510 |
| Msa1135380 | MtrunA17_Chr7g0275011 | 65.940 | 367 | 110 | 2 | 1 | 355 | 5 | 368 | 0.0 | 509 |
| Msa1135380 | MtrunA17_Chr7g0274991 | 66.116 | 363 | 108 | 3 | 7 | 357 | 10 | 369 | 7.70e-176 | 493 |
| Msa1135380 | MtrunA17_Chr1g0189931 | 65.970 | 335 | 102 | 1 | 32 | 354 | 31 | 365 | 6.35e-173 | 485 |
| Msa1135380 | MtrunA17_Chr7g0275001 | 66.667 | 342 | 101 | 2 | 29 | 357 | 21 | 362 | 1.64e-168 | 474 |
| Msa1135380 | MtrunA17_Chr1g0189901 | 64.095 | 337 | 109 | 1 | 31 | 355 | 29 | 365 | 6.52e-168 | 473 |
| Msa1135380 | MtrunA17_Chr4g0008851 | 62.778 | 360 | 118 | 3 | 12 | 357 | 13 | 370 | 1.24e-164 | 464 |
| Msa1135380 | MtrunA17_Chr1g0182811 | 58.172 | 361 | 132 | 4 | 11 | 357 | 16 | 371 | 5.15e-157 | 445 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1135380 | AT5G33370.1 | 66.859 | 347 | 101 | 2 | 23 | 357 | 22 | 366 | 9.82e-175 | 490 |
| Msa1135380 | AT3G04290.1 | 63.764 | 356 | 115 | 2 | 13 | 356 | 11 | 364 | 2.14e-172 | 484 |
| Msa1135380 | AT5G18430.1 | 63.912 | 363 | 113 | 4 | 5 | 353 | 4 | 362 | 1.81e-169 | 476 |
| Msa1135380 | AT4G28780.1 | 61.433 | 363 | 123 | 4 | 6 | 355 | 7 | 365 | 3.82e-162 | 458 |
| Msa1135380 | AT5G33370.2 | 64.207 | 271 | 83 | 2 | 23 | 281 | 22 | 290 | 1.48e-123 | 359 |
| Msa1135380 | AT2G23540.1 | 36.800 | 375 | 211 | 9 | 1 | 350 | 11 | 384 | 8.36e-78 | 244 |
| Msa1135380 | AT3G50400.1 | 37.772 | 368 | 208 | 10 | 1 | 347 | 1 | 368 | 1.23e-74 | 236 |
| Msa1135380 | AT5G15720.1 | 37.015 | 335 | 197 | 5 | 33 | 353 | 30 | 364 | 1.36e-68 | 219 |
| Msa1135380 | AT1G71691.2 | 37.391 | 345 | 193 | 7 | 22 | 354 | 51 | 384 | 2.58e-67 | 217 |
| Msa1135380 | AT1G33811.1 | 34.239 | 368 | 219 | 9 | 8 | 353 | 4 | 370 | 1.34e-64 | 209 |
Find 72 sgRNAs with CRISPR-Local
Find 288 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGCAAATAGACTTATTG+TGG | 0.517199 | 7_4:-2549099 | Msa1135380:CDS |
| AAAGGGCATAAACTTCTAAT+TGG | 0.258824 | 7_4:-2550441 | Msa1135380:CDS |
| AAAGTAGCTTGTTGTGGGCA+AGG | 0.536850 | 7_4:-2549215 | Msa1135380:CDS |
| AAATGTTACAAAGACTCAAT+AGG | 0.446396 | 7_4:-2549445 | Msa1135380:CDS |
| AACTTGACATAGTGTGGTAA+TGG | 0.474573 | 7_4:+2549832 | None:intergenic |
| AATACATACAAGTCTCTGTT+AGG | 0.315997 | 7_4:+2549146 | None:intergenic |
| AATTCCAGCTGAACCAAAAT+TGG | 0.235478 | 7_4:+2550416 | None:intergenic |
| ACAACCCAATGGTCCTGTTC+CGG | 0.513728 | 7_4:+2549559 | None:intergenic |
| ACAGAGACTTGTATGTATTT+TGG | 0.108906 | 7_4:-2549142 | Msa1135380:CDS |
| ACATCAAAAGTAGCTTGTTG+TGG | 0.536147 | 7_4:-2549221 | Msa1135380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTGGATAATCAATTCCATA+AGG | + | chr7_4:2549129-2549148 | None:intergenic | 30.0% | |
| AAATGGGAAGCAATATTTGT+TGG | + | chr7_4:2549314-2549333 | None:intergenic | 30.0% | |
| TTCTCTAGACATTGAATTTG+AGG | - | chr7_4:2549350-2549369 | Msa1135380:intron | 30.0% | |
| GAAAGTAACCAGTAAATTTG+AGG | + | chr7_4:2549565-2549584 | None:intergenic | 30.0% | |
| TTTAATTCTGGGCTCAAATA+TGG | + | chr7_4:2549775-2549794 | None:intergenic | 30.0% | |
| TATTTGAGCCCAGAATTAAA+AGG | - | chr7_4:2549775-2549794 | Msa1135380:intron | 30.0% | |
| ATTTGAGCCCAGAATTAAAA+GGG | - | chr7_4:2549776-2549795 | Msa1135380:intron | 30.0% | |
| ATTGGAATCCTTAATGATAC+AGG | - | chr7_4:2549838-2549857 | Msa1135380:CDS | 30.0% | |
| ATTGATCTCCTGTATCATTA+AGG | + | chr7_4:2549849-2549868 | None:intergenic | 30.0% | |
| TGCATTATATGAACTGGTAT+AGG | + | chr7_4:2549911-2549930 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 2548949 | 2551307 | 2548949 | ID=Msa1135380;Name=Msa1135380 |
| chr7_4 | mRNA | 2548949 | 2551307 | 2548949 | ID=Msa1135380-mRNA-1;Parent=Msa1135380;Name=Msa1135380-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.75|0.8|1|0.75|0.8|5|70|357 |
| chr7_4 | exon | 2548949 | 2549251 | 2548949 | ID=Msa1135380-mRNA-1:exon:833;Parent=Msa1135380-mRNA-1 |
| chr7_4 | exon | 2549366 | 2549618 | 2549366 | ID=Msa1135380-mRNA-1:exon:832;Parent=Msa1135380-mRNA-1 |
| chr7_4 | exon | 2549801 | 2550043 | 2549801 | ID=Msa1135380-mRNA-1:exon:831;Parent=Msa1135380-mRNA-1 |
| chr7_4 | exon | 2550386 | 2550513 | 2550386 | ID=Msa1135380-mRNA-1:exon:830;Parent=Msa1135380-mRNA-1 |
| chr7_4 | exon | 2551091 | 2551307 | 2551091 | ID=Msa1135380-mRNA-1:exon:829;Parent=Msa1135380-mRNA-1 |
| chr7_4 | CDS | 2551091 | 2551307 | 2551091 | ID=Msa1135380-mRNA-1:cds;Parent=Msa1135380-mRNA-1 |
| chr7_4 | CDS | 2550386 | 2550513 | 2550386 | ID=Msa1135380-mRNA-1:cds;Parent=Msa1135380-mRNA-1 |
| chr7_4 | CDS | 2549801 | 2550043 | 2549801 | ID=Msa1135380-mRNA-1:cds;Parent=Msa1135380-mRNA-1 |
| chr7_4 | CDS | 2549366 | 2549618 | 2549366 | ID=Msa1135380-mRNA-1:cds;Parent=Msa1135380-mRNA-1 |
| chr7_4 | CDS | 2549019 | 2549251 | 2549019 | ID=Msa1135380-mRNA-1:cds;Parent=Msa1135380-mRNA-1 |
| chr7_4 | three_prime_UTR | 2548949 | 2549018 | 2548949 | ID=Msa1135380-mRNA-1:three_prime_utr;Parent=Msa1135380-mRNA-1 |
| Gene Sequence |
>Msa1135380 ATGGCACCATCTTCAAGTTTTGTTCATATTATGGTAGTTCTTAGTTTAGTTTTGGTGTTAGGTGGTATTGTTCCTAAAGGTGAAGCTAGGAGAAGAGCTTTTTTTGTATTTGGAGATTCACTTGTCGATAATGGAAATAACAACTATTTGCTTACTACTGCTCGTGCTGATTCTCCTCCTTATGGAATTGATTATCCAACTCGTAGACCAACTGGTCGTCAGCAACTTGGAACTGTGTCTACTTTACCATATTTGAGCCCAGAATTAAAAGGGCATAAACTTCTAATTGGTGCCAATTTTGGTTCAGCTGGAATTGGAATCCTTAATGATACAGGAGATCAATTTATAAATGTGGTAAAAATGCATACACAACTGGATTACTTTGAAGAGTATCAACGTCGATTAACGAATTTAATAGGCGGTCGGCGGACTAGAAGACTTGTTAATCAAGCATTGTTTCTCATGACAGTTGGAGGCAATGATTTTGTAAACAACTATTACCTTGTGCCTTCTTCTTCAAGGTCTCGCCAATATCCATTACCACACTATGTCAAGTTCCTCATTTGTGAGTACAGAAAGCACTTACAGAGGCTATATGATTTGGGCGCTCGGCGAGTTCTAGTCACCGGAACAGGACCATTGGGTTGTGCTCCTGCAGAACTCGCTACGCGTAGCAGAAATGGTGAATGCTCGGTGGAACTACAACGTGGTGCAGCATTATACAACCCTCAACTTGAACAAATGTTACAAAGACTCAATAGGAAACTTGGCAGTGACATTTTCATTGCTGCAAATACAGCACAAATGCACAAAGATTTTGTTACTAATCCAACAGCTTATGGATTTGTTACATCAAAAGTAGCTTGTTGTGGGCAAGGACCCTATAATGGCATTGGAGTGTGCACAACATGCTCCAACTTGTGCCCTAACAGAGACTTGTATGTATTTTGGGATCCATTCCATCCATCTGAAAAGGCAAATAGACTTATTGTGGAGCAAATTATGTCAGGTTCAACAAGATACATGAAGCCAATGAACCTTAGCACCATTCTTTCTTTGGATGCTAGCACATGA |
| Protein sequence |
>Msa1135380 MAPSSSFVHIMVVLSLVLVLGGIVPKGEARRRAFFVFGDSLVDNGNNNYLLTTARADSPPYGIDYPTRRPTGRQQLGTVSTLPYLSPELKGHKLLIGANFGSAGIGILNDTGDQFINVVKMHTQLDYFEEYQRRLTNLIGGRRTRRLVNQALFLMTVGGNDFVNNYYLVPSSSRSRQYPLPHYVKFLICEYRKHLQRLYDLGARRVLVTGTGPLGCAPAELATRSRNGECSVELQRGAALYNPQLEQMLQRLNRKLGSDIFIAANTAQMHKDFVTNPTAYGFVTSKVACCGQGPYNGIGVCTTCSNLCPNRDLYVFWDPFHPSEKANRLIVEQIMSGSTRYMKPMNLSTILSLDAST* |
