AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1342380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1342380 MtrunA17_Chr3g0103421 85.616 146 15 2 1 144 1 142 9.17e-66 196
Msa1342380 MtrunA17_Chr5g0447241 52.542 118 35 4 27 144 20 116 4.53e-26 95.5
Msa1342380 MtrunA17_Chr1g0173641 45.977 87 43 3 62 144 16 102 4.92e-16 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1342380 AT3G20898.1 61.111 54 21 0 88 141 50 103 1.38e-17 73.6
Msa1342380 AT1G51355.1 57.895 57 22 1 88 144 56 110 3.16e-17 72.8

Find 20 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGAAGTTTGTTCTTCT+AGG 0.207635 tig0005557:+22651 None:intergenic
CACCAAAAGGTCAGAAATTT+AGG 0.220093 tig0005557:-22461 Msa1342380:CDS
ATCCTAAATTTCTGACCTTT+TGG 0.224238 tig0005557:+22459 None:intergenic
GAAACTACCCTTTGTTTCTT+TGG 0.243329 tig0005557:+22408 None:intergenic
ATTCTTTGTACCTCCTGATT+TGG 0.303744 tig0005557:-22355 Msa1342380:CDS
ACCCTTTGTTTCTTTGGTGC+TGG 0.336817 tig0005557:+22414 None:intergenic
TTTGATGCTGTTCTTCCAGA+AGG 0.463715 tig0005557:+22735 None:intergenic
GGAGGACATGTTGAAATCTC+TGG 0.487392 tig0005557:+22435 None:intergenic
TTGATGCTGTTCTTCCAGAA+GGG 0.495665 tig0005557:+22736 None:intergenic
CTTTGTTTCTTTGGTGCTGG+AGG 0.505878 tig0005557:+22417 None:intergenic
TTTCAAATTGTTCTTTGAGA+AGG 0.508424 tig0005557:-22389 Msa1342380:CDS
TTCCTTTGAAGACTCTTGAG+AGG 0.511869 tig0005557:+22548 None:intergenic
TGATGCTGTTCTTCCAGAAG+GGG 0.536691 tig0005557:+22737 None:intergenic
AGTGCATGTTCAACACCAAA+AGG 0.539127 tig0005557:-22474 Msa1342380:CDS
GGAGGTACAAAGAATGAAAG+TGG 0.568095 tig0005557:+22363 None:intergenic
CTCCAGCACCAAAGAAACAA+AGG 0.574035 tig0005557:-22416 Msa1342380:CDS
AACCTCTCAAGAGTCTTCAA+AGG 0.577571 tig0005557:-22550 Msa1342380:CDS
TCCAGCACCAAAGAAACAAA+GGG 0.597503 tig0005557:-22415 Msa1342380:CDS
CAGCATCAAACAAAATCTCA+AGG 0.603787 tig0005557:-22722 Msa1342380:CDS
AGAGAAGACTGAAATTTCTG+AGG 0.632052 tig0005557:-22526 Msa1342380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTCTTGTTGTAGTTTTTT+TGG + tig0005557:22375-22394 None:intergenic 25.0%
! AAAAAAACTACAACAAGAAC+AGG - tig0005557:22374-22393 Msa1342380:CDS 25.0%
!! TTTCAAATTGTTCTTTGAGA+AGG - tig0005557:22681-22700 Msa1342380:CDS 25.0%
! TTCTTGAAGTTTGTTCTTCT+AGG + tig0005557:22422-22441 None:intergenic 30.0%
! ATCCTAAATTTCTGACCTTT+TGG + tig0005557:22614-22633 None:intergenic 30.0%
CAGCATCAAACAAAATCTCA+AGG - tig0005557:22348-22367 Msa1342380:CDS 35.0%
!! GTTTTTGAAAGACAGCTAGA+AGG + tig0005557:22503-22522 None:intergenic 35.0%
AGAGAAGACTGAAATTTCTG+AGG - tig0005557:22544-22563 Msa1342380:CDS 35.0%
CACCAAAAGGTCAGAAATTT+AGG - tig0005557:22609-22628 Msa1342380:CDS 35.0%
! GAAACTACCCTTTGTTTCTT+TGG + tig0005557:22665-22684 None:intergenic 35.0%
ATTCTTTGTACCTCCTGATT+TGG - tig0005557:22715-22734 Msa1342380:CDS 35.0%
!! AAGAAAAAGTGCTCCAAATC+AGG + tig0005557:22731-22750 None:intergenic 35.0%
!!! TGATTTGGAGCACTTTTTCT+TGG - tig0005557:22730-22749 Msa1342380:CDS 35.0%
TTGATGCTGTTCTTCCAGAA+GGG + tig0005557:22337-22356 None:intergenic 40.0%
TTTGATGCTGTTCTTCCAGA+AGG + tig0005557:22338-22357 None:intergenic 40.0%
AACCTCTCAAGAGTCTTCAA+AGG - tig0005557:22520-22539 Msa1342380:CDS 40.0%
! TTCCTTTGAAGACTCTTGAG+AGG + tig0005557:22525-22544 None:intergenic 40.0%
AGTGCATGTTCAACACCAAA+AGG - tig0005557:22596-22615 Msa1342380:CDS 40.0%
TCCAGCACCAAAGAAACAAA+GGG - tig0005557:22655-22674 Msa1342380:CDS 40.0%
! GGAGGTACAAAGAATGAAAG+TGG + tig0005557:22710-22729 None:intergenic 40.0%
!! AAAAAGTGCTCCAAATCAGG+AGG + tig0005557:22728-22747 None:intergenic 40.0%
TGATGCTGTTCTTCCAGAAG+GGG + tig0005557:22336-22355 None:intergenic 45.0%
GGAGGACATGTTGAAATCTC+TGG + tig0005557:22638-22657 None:intergenic 45.0%
!! CTTTGTTTCTTTGGTGCTGG+AGG + tig0005557:22656-22675 None:intergenic 45.0%
CTCCAGCACCAAAGAAACAA+AGG - tig0005557:22654-22673 Msa1342380:CDS 45.0%
! ACCCTTTGTTTCTTTGGTGC+TGG + tig0005557:22659-22678 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
tig0005557 gene 22329 22763 22329 ID=Msa1342380;Name=Msa1342380
tig0005557 mRNA 22329 22763 22329 ID=Msa1342380-mRNA-1;Parent=Msa1342380;Name=Msa1342380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|144
tig0005557 exon 22329 22763 22329 ID=Msa1342380-mRNA-1:exon:3593;Parent=Msa1342380-mRNA-1
tig0005557 CDS 22329 22763 22329 ID=Msa1342380-mRNA-1:cds;Parent=Msa1342380-mRNA-1
Gene Sequence

>Msa1342380

ATGGCCCCTTCTGGAAGAACAGCATCAAACAAAATCTCAAGGACCAAAAAAACTACAACAAGAACAGGAACAAGAACAAGAACAAGACAACCTAGAAGAACAAACTTCAAGAACAAGAAGCAGCAAAGTAGTACTCATAGCAAAAAACAAGAGATAATTAACAGTGTTTTACCTTCTAGCTGTCTTTCAAAAACCTCTCAAGAGTCTTCAAAGGAAGAGAAGACTGAAATTTCTGAGGTAATTGATGTTTCTAACAATAACAGTAACAGTGCATGTTCAACACCAAAAGGTCAGAAATTTAGGATACCAGAGATTTCAACATGTCCTCCAGCACCAAAGAAACAAAGGGTAGTTTCAAATTGTTCTTTGAGAAGGTCACCACTTTCATTCTTTGTACCTCCTGATTTGGAGCACTTTTTCTTGGTGACACTTTGA

Protein sequence

>Msa1342380

MAPSGRTASNKISRTKKTTTRTGTRTRTRQPRRTNFKNKKQQSSTHSKKQEIINSVLPSSCLSKTSQESSKEEKTEISEVIDVSNNNSNSACSTPKGQKFRIPEISTCPPAPKKQRVVSNCSLRRSPLSFFVPPDLEHFFLVTL*