AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1239440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1239440 MtrunA17_Chr8g0351111 96.933 326 10 0 1 326 1 326 0.0 651
Msa1239440 MtrunA17_Chr8g0351121 59.875 319 126 2 4 322 42 358 2.98e-137 393
Msa1239440 MtrunA17_Chr8g0351141 58.805 318 130 1 5 322 9 325 5.45e-136 389
Msa1239440 MtrunA17_Chr7g0246361 52.234 291 123 6 36 324 15 291 1.15e-99 295
Msa1239440 MtrunA17_Chr4g0004681 44.840 281 147 2 42 322 37 309 2.16e-89 270
Msa1239440 MtrunA17_Chr4g0004711 43.871 310 154 5 20 322 16 312 2.30e-89 270
Msa1239440 MtrunA17_Chr4g0004721 45.907 281 147 2 42 322 38 313 4.18e-88 266
Msa1239440 MtrunA17_Chr2g0331641 45.130 308 158 5 18 322 14 313 7.45e-80 245
Msa1239440 MtrunA17_Chr4g0004741 44.128 281 152 2 42 322 37 312 3.43e-73 228
Msa1239440 MtrunA17_Chr3g0077771 27.007 274 175 5 65 318 107 375 3.99e-28 112
Msa1239440 MtrunA17_Chr4g0000511 29.658 263 170 5 76 326 152 411 4.39e-25 105
Msa1239440 MtrunA17_Chr1g0208141 28.517 263 173 5 76 326 119 378 1.49e-23 101
Msa1239440 MtrunA17_Chr1g0210971 28.016 257 155 7 83 312 94 347 1.25e-22 97.1
Msa1239440 MtrunA17_Chr6g0450811 27.027 259 174 4 65 313 111 364 6.53e-22 95.1
Msa1239440 MtrunA17_Chr6g0450821 25.197 254 166 4 65 313 81 315 8.85e-17 80.1
Msa1239440 MtrunA17_Chr7g0248631 26.201 229 154 4 98 315 97 321 1.00e-16 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1239440 AT2G45630.2 50.765 327 146 5 3 322 19 337 2.94e-110 324
Msa1239440 AT1G12550.1 50.000 324 149 4 6 324 7 322 9.89e-106 312
Msa1239440 AT1G79870.1 43.492 315 170 4 8 322 6 312 9.98e-83 253
Msa1239440 AT1G79870.2 40.000 315 162 4 8 322 6 293 2.72e-72 226
Msa1239440 AT2G45630.1 43.590 156 74 4 3 152 19 166 2.05e-32 119
Msa1239440 AT1G72190.1 26.562 320 203 8 4 306 50 354 2.73e-28 113
Msa1239440 AT1G17745.1 28.521 284 187 7 56 326 125 405 3.28e-26 109
Msa1239440 AT4G34200.1 30.682 264 166 6 76 326 125 384 8.17e-26 108
Msa1239440 AT5G28310.1 35.359 181 64 4 144 322 103 232 1.15e-24 100
Msa1239440 AT3G19480.1 31.395 258 158 7 76 319 110 362 9.24e-24 102
Msa1239440 AT1G17745.2 26.688 311 185 8 56 326 125 432 5.17e-23 100
Msa1239440 AT5G14780.3 26.639 244 162 5 81 313 99 336 5.81e-22 95.5
Msa1239440 AT5G14780.2 26.639 244 162 5 81 313 111 348 6.01e-22 95.5
Msa1239440 AT5G14780.1 26.639 244 162 5 81 313 125 362 8.59e-22 95.1
Msa1239440 AT1G68010.3 27.519 258 166 3 83 319 94 351 1.64e-20 91.3
Msa1239440 AT1G68010.1 27.559 254 160 4 83 312 94 347 1.99e-20 91.3
Msa1239440 AT1G68010.2 28.516 256 156 7 83 312 94 348 7.81e-19 86.7
Msa1239440 AT1G01510.1 26.180 233 153 5 98 315 105 333 3.23e-14 73.9

Find 92 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCAAAAGTACTTGTTCT+TGG 0.227157 8_2:-21187726 Msa1239440:CDS
AGTGATAGGCCTGAGGGTTT+GGG 0.244134 8_2:+21187642 None:intergenic
GGCCTGAGGGTTTGGGGTTT+AGG 0.249977 8_2:+21187649 None:intergenic
CTCGGAGGATGTGGCTGATA+TGG 0.258496 8_2:-21187415 Msa1239440:CDS
CAGTGATAGGCCTGAGGGTT+TGG 0.272695 8_2:+21187641 None:intergenic
GATCCTTGGGATTTCCCTCT+TGG 0.293816 8_2:-21187315 Msa1239440:CDS
TATAAACAACGATGTCCTTT+TGG 0.311089 8_2:-21186606 Msa1239440:CDS
CGCGACGACGGCATTCATTC+AGG 0.317773 8_2:+21187469 None:intergenic
GGCGGGAACGTTCTGATCCT+TGG 0.323599 8_2:-21187329 Msa1239440:CDS
ACACTCAAAATTGTGGTTAC+TGG 0.327143 8_2:+21187817 None:intergenic
ATGCCGTCGTCGCGGAATTC+AGG 0.333564 8_2:-21187460 Msa1239440:CDS
CGAGTTGGAATTGTTGGATT+AGG 0.338569 8_2:-21186806 Msa1239440:CDS
ATATGCAGCGCTACAGAATA+TGG 0.355416 8_2:+21187572 None:intergenic
GGCAAGCGAGTTGGAATTGT+TGG 0.356871 8_2:-21186812 Msa1239440:CDS
ACAATTGATCAAGTGTTTGA+TGG 0.359013 8_2:-21186525 Msa1239440:CDS
GGAATTCAGGTTGCCGGTGC+TGG 0.372744 8_2:-21187447 Msa1239440:CDS
GGACCAAGAACAAGTACTTT+TGG 0.377384 8_2:+21187723 None:intergenic
AGAAATTGCTGGTGCTGGTT+TGG 0.382247 8_2:-21186498 Msa1239440:CDS
GAAGGGTTTATTGTAAATGT+TGG 0.390642 8_2:-21186572 Msa1239440:CDS
ACTGATTCTCTCATGGGATT+AGG 0.393926 8_2:-21186291 Msa1239440:three_prime_UTR
ATTGTTGGATTAGGAAGTAT+TGG 0.399827 8_2:-21186797 Msa1239440:CDS
TTGTTGATGGATTGTGGTTG+TGG 0.413973 8_2:+21187601 None:intergenic
GCGGGAACGTTCTGATCCTT+GGG 0.415107 8_2:-21187328 Msa1239440:CDS
GGATTTCCCTCTTGGATACA+AGG 0.415287 8_2:-21187307 Msa1239440:intron
GGATGAATTGTTGCAGTGAT+AGG 0.422185 8_2:+21187628 None:intergenic
TGTTTATAATGTGTCGTGTT+TGG 0.423029 8_2:+21186621 None:intergenic
AATATGGCGGGAATTGTTGA+TGG 0.425691 8_2:+21187588 None:intergenic
ATGGATTGTGGTTGTGGGAA+AGG 0.445567 8_2:+21187607 None:intergenic
TCAATAACATTGGAATAGAA+AGG 0.449303 8_2:+21186692 None:intergenic
AACTTCACTGGACTAATTAG+TGG 0.456808 8_2:+21186323 None:intergenic
CGGGAATTGTTGATGGATTG+TGG 0.457939 8_2:+21187595 None:intergenic
GAGACACAATAATAATTAAG+AGG 0.460135 8_2:-21187861 Msa1239440:five_prime_UTR
ACTAATTAGTCCAGTGAAGT+TGG 0.461367 8_2:-21186321 Msa1239440:CDS
GGATTTGTGGTCTTTGAGAA+AGG 0.465144 8_2:+21187771 None:intergenic
AATGAAACTAGTACTGTTTG+AGG 0.469780 8_2:+21187794 None:intergenic
TTTATTGTAAATGTTGGAAG+AGG 0.472073 8_2:-21186566 Msa1239440:CDS
CGTCGCGGAATTCAGGTTGC+CGG 0.475850 8_2:-21187453 Msa1239440:CDS
CACAAATCCTTAAAATTCAA+TGG 0.478465 8_2:-21187757 Msa1239440:exon
TGTTGATGGATTGTGGTTGT+GGG 0.478601 8_2:+21187602 None:intergenic
CAAGGCTGAGCGAGGGAAGA+AGG 0.482908 8_2:+21187526 None:intergenic
TTGAGTAACTGATTCTCTCA+TGG 0.492925 8_2:-21186298 Msa1239440:exon
ATTGTAAATGTTGGAAGAGG+TGG 0.499918 8_2:-21186563 Msa1239440:CDS
TGTCATGAGGAAGGTGACTA+TGG 0.499990 8_2:-21187373 Msa1239440:CDS
GTTGAGAACGTCGGTAGTGA+TGG 0.509803 8_2:+21187548 None:intergenic
GTGATAGGCCTGAGGGTTTG+GGG 0.518270 8_2:+21187643 None:intergenic
TTCCTAAACCCCAAACCCTC+AGG 0.518357 8_2:-21187651 Msa1239440:CDS
AGAGTAGAGTGATTCGAAGG+TGG 0.520358 8_2:+21187686 None:intergenic
GCTCTTTGCAATGGATAATG+TGG 0.520923 8_2:-21186441 Msa1239440:CDS
TGAGTAACTGATTCTCTCAT+GGG 0.521630 8_2:-21186297 Msa1239440:exon
CTTGTTGTCACCTCCAGTGT+TGG 0.525756 8_2:-21187507 Msa1239440:CDS
AGTTACTCAACCAACTTCAC+TGG 0.527156 8_2:+21186311 None:intergenic
TAGTTATCAGGCAAGCGAGT+TGG 0.543767 8_2:-21186821 Msa1239440:CDS
CAATAACATTGGAATAGAAA+GGG 0.548282 8_2:+21186693 None:intergenic
GTGAGAGTAGAGTGATTCGA+AGG 0.550168 8_2:+21187683 None:intergenic
TTGATCAAGTGTTTGATGGA+AGG 0.551467 8_2:-21186521 Msa1239440:CDS
GAAGGAGAAATTGCTGGTGC+TGG 0.558068 8_2:-21186503 Msa1239440:CDS
AGTTTGTGTGAACTTGTGGC+AGG 0.560200 8_2:-21186374 Msa1239440:CDS
ACAGTAAAAGCTGCAGAATG+TGG 0.564794 8_2:+21186410 None:intergenic
ATTCAGGTTGCCGGTGCTGG+AGG 0.565610 8_2:-21187444 Msa1239440:CDS
AAGGTGGAGAAGCATGTTGG+TGG 0.565774 8_2:+21187702 None:intergenic
AACTTCTAGCGACGCACTTG+TGG 0.568567 8_2:-21186663 Msa1239440:CDS
TTTGAGGACACTCAAAATTG+TGG 0.571306 8_2:+21187810 None:intergenic
TGATGAAAAGCTCTTTGCAA+TGG 0.579661 8_2:-21186450 Msa1239440:CDS
TTGATGGAAGGAGAAATTGC+TGG 0.584407 8_2:-21186509 Msa1239440:CDS
AGTTGCAAGATCAATAACAT+TGG 0.588695 8_2:+21186682 None:intergenic
AATGTGATCAGTTCCAACAC+TGG 0.588992 8_2:+21187494 None:intergenic
CAACCTGAATTCCGCGACGA+CGG 0.589863 8_2:+21187457 None:intergenic
CGAGAAAACTCCTCCAGCAC+CGG 0.593259 8_2:+21187434 None:intergenic
TGGATTAGGAAGTATTGGCA+TGG 0.593545 8_2:-21186792 Msa1239440:CDS
CGGATCGATATGTGAGGACG+CGG 0.601075 8_2:-21187350 Msa1239440:CDS
GCAGCGCTACAGAATATGGC+GGG 0.604474 8_2:+21187576 None:intergenic
TGTTGCAGTGATAGGCCTGA+GGG 0.610418 8_2:+21187636 None:intergenic
GGTGACAACAAGGCTGAGCG+AGG 0.616021 8_2:+21187518 None:intergenic
AATGAGTTTGTGTGAACTTG+TGG 0.617149 8_2:-21186378 Msa1239440:CDS
CGTTGCTGATTGCTGTCATG+AGG 0.622722 8_2:-21187386 Msa1239440:CDS
TGTGAACCTTGTATCCAAGA+GGG 0.623359 8_2:+21187301 None:intergenic
ATCGATATGTGAGGACGCGG+CGG 0.627272 8_2:-21187347 Msa1239440:CDS
CAACACTGGAGGTGACAACA+AGG 0.627680 8_2:+21187508 None:intergenic
CATGAGGAAGGTGACTATGG+CGG 0.631252 8_2:-21187370 Msa1239440:CDS
ATGTGAACCTTGTATCCAAG+AGG 0.641365 8_2:+21187300 None:intergenic
CTGAATGAATGCCGTCGTCG+CGG 0.646790 8_2:-21187468 Msa1239440:CDS
TATCCAAGAGGGAAATCCCA+AGG 0.655740 8_2:+21187312 None:intergenic
GCTGATTGCTGTCATGAGGA+AGG 0.663926 8_2:-21187382 Msa1239440:CDS
GTGACAACAAGGCTGAGCGA+GGG 0.665503 8_2:+21187519 None:intergenic
TGCAGCGCTACAGAATATGG+CGG 0.674749 8_2:+21187575 None:intergenic
TTGTTGCAGTGATAGGCCTG+AGG 0.677465 8_2:+21187635 None:intergenic
GTTTGTGTGAACTTGTGGCA+GGG 0.679026 8_2:-21186373 Msa1239440:CDS
TCGAAGGTGGAGAAGCATGT+TGG 0.681312 8_2:+21187699 None:intergenic
GTGATCAGTTCCAACACTGG+AGG 0.687001 8_2:+21187497 None:intergenic
GGAGGATGTGGCTGATATGG+CGG 0.694931 8_2:-21187412 Msa1239440:CDS
GGGAAGAAGGTTGAGAACGT+CGG 0.695377 8_2:+21187539 None:intergenic
CTATGGCGGATCGATATGTG+AGG 0.738984 8_2:-21187356 Msa1239440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTCCGATGTTAATT+TGG + chr8_2:21186898-21186917 None:intergenic 20.0%
!! AAATTTCAATTTCTCATGAA+TGG + chr8_2:21187046-21187065 None:intergenic 20.0%
!! ATGAGAAATTGAAATTTCTT+TGG - chr8_2:21187049-21187068 Msa1239440:intron 20.0%
!!! AATTTTTTCCGATGTTAATT+TGG + chr8_2:21186898-21186917 None:intergenic 20.0%
!! AAATTTCAATTTCTCATGAA+TGG + chr8_2:21187046-21187065 None:intergenic 20.0%
!! ATGAGAAATTGAAATTTCTT+TGG - chr8_2:21187049-21187068 Msa1239440:intron 20.0%
! GAGACACAATAATAATTAAG+AGG - chr8_2:21186234-21186253 Msa1239440:three_prime_UTR 25.0%
!!! CATTGAATTTTAAGGATTTG+TGG + chr8_2:21186340-21186359 None:intergenic 25.0%
! CACAAATCCTTAAAATTCAA+TGG - chr8_2:21186338-21186357 Msa1239440:CDS 25.0%
!!! TTTTTGGCCATTGAATTTTA+AGG + chr8_2:21186348-21186367 None:intergenic 25.0%
! TAACCTATCCAAATTAACAT+CGG - chr8_2:21186887-21186906 Msa1239440:intron 25.0%
!! AATCATATTGAAAGTGCATA+TGG - chr8_2:21187188-21187207 Msa1239440:intron 25.0%
!!! TTTTCACTGTTTTAGTTATC+AGG - chr8_2:21187262-21187281 Msa1239440:intron 25.0%
! CAATAACATTGGAATAGAAA+GGG + chr8_2:21187405-21187424 None:intergenic 25.0%
! TCAATAACATTGGAATAGAA+AGG + chr8_2:21187406-21187425 None:intergenic 25.0%
! TTTATTGTAAATGTTGGAAG+AGG - chr8_2:21187529-21187548 Msa1239440:CDS 25.0%
! GAGACACAATAATAATTAAG+AGG - chr8_2:21186234-21186253 Msa1239440:three_prime_UTR 25.0%
!!! CATTGAATTTTAAGGATTTG+TGG + chr8_2:21186340-21186359 None:intergenic 25.0%
! CACAAATCCTTAAAATTCAA+TGG - chr8_2:21186338-21186357 Msa1239440:CDS 25.0%
!!! TTTTTGGCCATTGAATTTTA+AGG + chr8_2:21186348-21186367 None:intergenic 25.0%
! TAACCTATCCAAATTAACAT+CGG - chr8_2:21186887-21186906 Msa1239440:intron 25.0%
!! AATCATATTGAAAGTGCATA+TGG - chr8_2:21187188-21187207 Msa1239440:intron 25.0%
!!! TTTTCACTGTTTTAGTTATC+AGG - chr8_2:21187262-21187281 Msa1239440:intron 25.0%
! CAATAACATTGGAATAGAAA+GGG + chr8_2:21187405-21187424 None:intergenic 25.0%
! TCAATAACATTGGAATAGAA+AGG + chr8_2:21187406-21187425 None:intergenic 25.0%
! TTTATTGTAAATGTTGGAAG+AGG - chr8_2:21187529-21187548 Msa1239440:CDS 25.0%
AATGAAACTAGTACTGTTTG+AGG + chr8_2:21186304-21186323 None:intergenic 30.0%
!! AAGTACTTTTGGAAGGTTTT+TGG + chr8_2:21186364-21186383 None:intergenic 30.0%
! TTTCCGATGTTAATTTGGAT+AGG + chr8_2:21186893-21186912 None:intergenic 30.0%
ACATGAAGTATATGTTGAAG+TGG + chr8_2:21187107-21187126 None:intergenic 30.0%
! ATTGTTGGATTAGGAAGTAT+TGG - chr8_2:21187298-21187317 Msa1239440:intron 30.0%
AGTTGCAAGATCAATAACAT+TGG + chr8_2:21187416-21187435 None:intergenic 30.0%
TGTTTATAATGTGTCGTGTT+TGG + chr8_2:21187477-21187496 None:intergenic 30.0%
! TATAAACAACGATGTCCTTT+TGG - chr8_2:21187489-21187508 Msa1239440:CDS 30.0%
! TTTTGGCATTAGGAAAAGAA+GGG - chr8_2:21187506-21187525 Msa1239440:CDS 30.0%
GAAGGGTTTATTGTAAATGT+TGG - chr8_2:21187523-21187542 Msa1239440:CDS 30.0%
ACAATTGATCAAGTGTTTGA+TGG - chr8_2:21187570-21187589 Msa1239440:CDS 30.0%
AATGAAACTAGTACTGTTTG+AGG + chr8_2:21186304-21186323 None:intergenic 30.0%
!! AAGTACTTTTGGAAGGTTTT+TGG + chr8_2:21186364-21186383 None:intergenic 30.0%
! TTTCCGATGTTAATTTGGAT+AGG + chr8_2:21186893-21186912 None:intergenic 30.0%
ACATGAAGTATATGTTGAAG+TGG + chr8_2:21187107-21187126 None:intergenic 30.0%
! ATTGTTGGATTAGGAAGTAT+TGG - chr8_2:21187298-21187317 Msa1239440:intron 30.0%
AGTTGCAAGATCAATAACAT+TGG + chr8_2:21187416-21187435 None:intergenic 30.0%
TGTTTATAATGTGTCGTGTT+TGG + chr8_2:21187477-21187496 None:intergenic 30.0%
! TATAAACAACGATGTCCTTT+TGG - chr8_2:21187489-21187508 Msa1239440:CDS 30.0%
! TTTTGGCATTAGGAAAAGAA+GGG - chr8_2:21187506-21187525 Msa1239440:CDS 30.0%
GAAGGGTTTATTGTAAATGT+TGG - chr8_2:21187523-21187542 Msa1239440:CDS 30.0%
ACAATTGATCAAGTGTTTGA+TGG - chr8_2:21187570-21187589 Msa1239440:CDS 30.0%
ACACTCAAAATTGTGGTTAC+TGG + chr8_2:21186281-21186300 None:intergenic 35.0%
TTTGAGGACACTCAAAATTG+TGG + chr8_2:21186288-21186307 None:intergenic 35.0%
! CAAGAACAAGTACTTTTGGA+AGG + chr8_2:21186371-21186390 None:intergenic 35.0%
!! CTTCCAAAAGTACTTGTTCT+TGG - chr8_2:21186369-21186388 Msa1239440:CDS 35.0%
!!! GAAGTGGTTTTTTTTTCTGC+TGG + chr8_2:21187091-21187110 None:intergenic 35.0%
! TGGTTCATCTCACATTTTGT+TGG + chr8_2:21187143-21187162 None:intergenic 35.0%
! GAAGCGACAATTCTATGTTT+TGG + chr8_2:21187163-21187182 None:intergenic 35.0%
! ATGTCTTTTGATGCTTAGAG+TGG + chr8_2:21187372-21187391 None:intergenic 35.0%
! CTTCTTTTCCTAATGCCAAA+AGG + chr8_2:21187507-21187526 None:intergenic 35.0%
! CTTTTGGCATTAGGAAAAGA+AGG - chr8_2:21187505-21187524 Msa1239440:CDS 35.0%
ATTGTAAATGTTGGAAGAGG+TGG - chr8_2:21187532-21187551 Msa1239440:CDS 35.0%
TTGATCAAGTGTTTGATGGA+AGG - chr8_2:21187574-21187593 Msa1239440:CDS 35.0%
! AAGAGCTTTTCATCAACATG+AGG + chr8_2:21187640-21187659 None:intergenic 35.0%
TGATGAAAAGCTCTTTGCAA+TGG - chr8_2:21187645-21187664 Msa1239440:CDS 35.0%
AATGAGTTTGTGTGAACTTG+TGG - chr8_2:21187717-21187736 Msa1239440:CDS 35.0%
AACTTCACTGGACTAATTAG+TGG + chr8_2:21187775-21187794 None:intergenic 35.0%
ACTAATTAGTCCAGTGAAGT+TGG - chr8_2:21187774-21187793 Msa1239440:five_prime_UTR 35.0%
! TTGAGTAACTGATTCTCTCA+TGG - chr8_2:21187797-21187816 Msa1239440:five_prime_UTR 35.0%
! TGAGTAACTGATTCTCTCAT+GGG - chr8_2:21187798-21187817 Msa1239440:five_prime_UTR 35.0%
ACACTCAAAATTGTGGTTAC+TGG + chr8_2:21186281-21186300 None:intergenic 35.0%
TTTGAGGACACTCAAAATTG+TGG + chr8_2:21186288-21186307 None:intergenic 35.0%
! CAAGAACAAGTACTTTTGGA+AGG + chr8_2:21186371-21186390 None:intergenic 35.0%
!! CTTCCAAAAGTACTTGTTCT+TGG - chr8_2:21186369-21186388 Msa1239440:CDS 35.0%
!!! GAAGTGGTTTTTTTTTCTGC+TGG + chr8_2:21187091-21187110 None:intergenic 35.0%
! TGGTTCATCTCACATTTTGT+TGG + chr8_2:21187143-21187162 None:intergenic 35.0%
! GAAGCGACAATTCTATGTTT+TGG + chr8_2:21187163-21187182 None:intergenic 35.0%
! ATGTCTTTTGATGCTTAGAG+TGG + chr8_2:21187372-21187391 None:intergenic 35.0%
! CTTCTTTTCCTAATGCCAAA+AGG + chr8_2:21187507-21187526 None:intergenic 35.0%
! CTTTTGGCATTAGGAAAAGA+AGG - chr8_2:21187505-21187524 Msa1239440:CDS 35.0%
ATTGTAAATGTTGGAAGAGG+TGG - chr8_2:21187532-21187551 Msa1239440:CDS 35.0%
TTGATCAAGTGTTTGATGGA+AGG - chr8_2:21187574-21187593 Msa1239440:CDS 35.0%
! AAGAGCTTTTCATCAACATG+AGG + chr8_2:21187640-21187659 None:intergenic 35.0%
TGATGAAAAGCTCTTTGCAA+TGG - chr8_2:21187645-21187664 Msa1239440:CDS 35.0%
AATGAGTTTGTGTGAACTTG+TGG - chr8_2:21187717-21187736 Msa1239440:CDS 35.0%
AACTTCACTGGACTAATTAG+TGG + chr8_2:21187775-21187794 None:intergenic 35.0%
ACTAATTAGTCCAGTGAAGT+TGG - chr8_2:21187774-21187793 Msa1239440:five_prime_UTR 35.0%
! TTGAGTAACTGATTCTCTCA+TGG - chr8_2:21187797-21187816 Msa1239440:five_prime_UTR 35.0%
! TGAGTAACTGATTCTCTCAT+GGG - chr8_2:21187798-21187817 Msa1239440:five_prime_UTR 35.0%
! GGATTTGTGGTCTTTGAGAA+AGG + chr8_2:21186327-21186346 None:intergenic 40.0%
! GGACCAAGAACAAGTACTTT+TGG + chr8_2:21186375-21186394 None:intergenic 40.0%
GGATGAATTGTTGCAGTGAT+AGG + chr8_2:21186470-21186489 None:intergenic 40.0%
!! TGTTGATGGATTGTGGTTGT+GGG + chr8_2:21186496-21186515 None:intergenic 40.0%
!! TTGTTGATGGATTGTGGTTG+TGG + chr8_2:21186497-21186516 None:intergenic 40.0%
! AATATGGCGGGAATTGTTGA+TGG + chr8_2:21186510-21186529 None:intergenic 40.0%
ATATGCAGCGCTACAGAATA+TGG + chr8_2:21186526-21186545 None:intergenic 40.0%
AATGTGATCAGTTCCAACAC+TGG + chr8_2:21186604-21186623 None:intergenic 40.0%
TGTGAACCTTGTATCCAAGA+GGG + chr8_2:21186797-21186816 None:intergenic 40.0%
ATGTGAACCTTGTATCCAAG+AGG + chr8_2:21186798-21186817 None:intergenic 40.0%
CTTCTACTTGCTTCTCTTCA+AGG + chr8_2:21186929-21186948 None:intergenic 40.0%
GAATATTAGTGTCTCGCTAC+TGG + chr8_2:21186974-21186993 None:intergenic 40.0%
! CGAGTTGGAATTGTTGGATT+AGG - chr8_2:21187289-21187308 Msa1239440:intron 40.0%
!! TGGATTAGGAAGTATTGGCA+TGG - chr8_2:21187303-21187322 Msa1239440:intron 40.0%
! AACGATGTCCTTTTGGCATT+AGG - chr8_2:21187496-21187515 Msa1239440:CDS 40.0%
TTGATGGAAGGAGAAATTGC+TGG - chr8_2:21187586-21187605 Msa1239440:CDS 40.0%
GCTCTTTGCAATGGATAATG+TGG - chr8_2:21187654-21187673 Msa1239440:CDS 40.0%
ACAGTAAAAGCTGCAGAATG+TGG + chr8_2:21187688-21187707 None:intergenic 40.0%
AGTTACTCAACCAACTTCAC+TGG + chr8_2:21187787-21187806 None:intergenic 40.0%
! ACTGATTCTCTCATGGGATT+AGG - chr8_2:21187804-21187823 Msa1239440:five_prime_UTR 40.0%
! GGATTTGTGGTCTTTGAGAA+AGG + chr8_2:21186327-21186346 None:intergenic 40.0%
! GGACCAAGAACAAGTACTTT+TGG + chr8_2:21186375-21186394 None:intergenic 40.0%
GGATGAATTGTTGCAGTGAT+AGG + chr8_2:21186470-21186489 None:intergenic 40.0%
!! TGTTGATGGATTGTGGTTGT+GGG + chr8_2:21186496-21186515 None:intergenic 40.0%
!! TTGTTGATGGATTGTGGTTG+TGG + chr8_2:21186497-21186516 None:intergenic 40.0%
! AATATGGCGGGAATTGTTGA+TGG + chr8_2:21186510-21186529 None:intergenic 40.0%
ATATGCAGCGCTACAGAATA+TGG + chr8_2:21186526-21186545 None:intergenic 40.0%
AATGTGATCAGTTCCAACAC+TGG + chr8_2:21186604-21186623 None:intergenic 40.0%
TGTGAACCTTGTATCCAAGA+GGG + chr8_2:21186797-21186816 None:intergenic 40.0%
ATGTGAACCTTGTATCCAAG+AGG + chr8_2:21186798-21186817 None:intergenic 40.0%
CTTCTACTTGCTTCTCTTCA+AGG + chr8_2:21186929-21186948 None:intergenic 40.0%
GAATATTAGTGTCTCGCTAC+TGG + chr8_2:21186974-21186993 None:intergenic 40.0%
! CGAGTTGGAATTGTTGGATT+AGG - chr8_2:21187289-21187308 Msa1239440:intron 40.0%
!! TGGATTAGGAAGTATTGGCA+TGG - chr8_2:21187303-21187322 Msa1239440:intron 40.0%
! AACGATGTCCTTTTGGCATT+AGG - chr8_2:21187496-21187515 Msa1239440:CDS 40.0%
TTGATGGAAGGAGAAATTGC+TGG - chr8_2:21187586-21187605 Msa1239440:CDS 40.0%
GCTCTTTGCAATGGATAATG+TGG - chr8_2:21187654-21187673 Msa1239440:CDS 40.0%
ACAGTAAAAGCTGCAGAATG+TGG + chr8_2:21187688-21187707 None:intergenic 40.0%
AGTTACTCAACCAACTTCAC+TGG + chr8_2:21187787-21187806 None:intergenic 40.0%
! ACTGATTCTCTCATGGGATT+AGG - chr8_2:21187804-21187823 Msa1239440:five_prime_UTR 40.0%
AGAGTAGAGTGATTCGAAGG+TGG + chr8_2:21186412-21186431 None:intergenic 45.0%
GTGAGAGTAGAGTGATTCGA+AGG + chr8_2:21186415-21186434 None:intergenic 45.0%
! ATGGATTGTGGTTGTGGGAA+AGG + chr8_2:21186491-21186510 None:intergenic 45.0%
!! CGGGAATTGTTGATGGATTG+TGG + chr8_2:21186503-21186522 None:intergenic 45.0%
TGTCATGAGGAAGGTGACTA+TGG - chr8_2:21186722-21186741 Msa1239440:CDS 45.0%
TATCCAAGAGGGAAATCCCA+AGG + chr8_2:21186786-21186805 None:intergenic 45.0%
GGATTTCCCTCTTGGATACA+AGG - chr8_2:21186788-21186807 Msa1239440:CDS 45.0%
ACTTGCTTCTCTTCAAGGTC+TGG + chr8_2:21186924-21186943 None:intergenic 45.0%
GCGCAACAAACATGCACTTA+AGG - chr8_2:21187233-21187252 Msa1239440:intron 45.0%
TAGTTATCAGGCAAGCGAGT+TGG - chr8_2:21187274-21187293 Msa1239440:intron 45.0%
!! AGAAATTGCTGGTGCTGGTT+TGG - chr8_2:21187597-21187616 Msa1239440:CDS 45.0%
AGTTTGTGTGAACTTGTGGC+AGG - chr8_2:21187721-21187740 Msa1239440:CDS 45.0%
GTTTGTGTGAACTTGTGGCA+GGG - chr8_2:21187722-21187741 Msa1239440:CDS 45.0%
AGAGTAGAGTGATTCGAAGG+TGG + chr8_2:21186412-21186431 None:intergenic 45.0%
GTGAGAGTAGAGTGATTCGA+AGG + chr8_2:21186415-21186434 None:intergenic 45.0%
! ATGGATTGTGGTTGTGGGAA+AGG + chr8_2:21186491-21186510 None:intergenic 45.0%
!! CGGGAATTGTTGATGGATTG+TGG + chr8_2:21186503-21186522 None:intergenic 45.0%
TGTCATGAGGAAGGTGACTA+TGG - chr8_2:21186722-21186741 Msa1239440:CDS 45.0%
TATCCAAGAGGGAAATCCCA+AGG + chr8_2:21186786-21186805 None:intergenic 45.0%
GGATTTCCCTCTTGGATACA+AGG - chr8_2:21186788-21186807 Msa1239440:CDS 45.0%
ACTTGCTTCTCTTCAAGGTC+TGG + chr8_2:21186924-21186943 None:intergenic 45.0%
GCGCAACAAACATGCACTTA+AGG - chr8_2:21187233-21187252 Msa1239440:intron 45.0%
TAGTTATCAGGCAAGCGAGT+TGG - chr8_2:21187274-21187293 Msa1239440:intron 45.0%
!! AGAAATTGCTGGTGCTGGTT+TGG - chr8_2:21187597-21187616 Msa1239440:CDS 45.0%
AGTTTGTGTGAACTTGTGGC+AGG - chr8_2:21187721-21187740 Msa1239440:CDS 45.0%
GTTTGTGTGAACTTGTGGCA+GGG - chr8_2:21187722-21187741 Msa1239440:CDS 45.0%
! AAGGTGGAGAAGCATGTTGG+TGG + chr8_2:21186396-21186415 None:intergenic 50.0%
! TCGAAGGTGGAGAAGCATGT+TGG + chr8_2:21186399-21186418 None:intergenic 50.0%
TTCCTAAACCCCAAACCCTC+AGG - chr8_2:21186444-21186463 Msa1239440:CDS 50.0%
AGTGATAGGCCTGAGGGTTT+GGG + chr8_2:21186456-21186475 None:intergenic 50.0%
TGTTGCAGTGATAGGCCTGA+GGG + chr8_2:21186462-21186481 None:intergenic 50.0%
TTGTTGCAGTGATAGGCCTG+AGG + chr8_2:21186463-21186482 None:intergenic 50.0%
TGCAGCGCTACAGAATATGG+CGG + chr8_2:21186523-21186542 None:intergenic 50.0%
GTTGAGAACGTCGGTAGTGA+TGG + chr8_2:21186550-21186569 None:intergenic 50.0%
GGGAAGAAGGTTGAGAACGT+CGG + chr8_2:21186559-21186578 None:intergenic 50.0%
CAACACTGGAGGTGACAACA+AGG + chr8_2:21186590-21186609 None:intergenic 50.0%
! CTTGTTGTCACCTCCAGTGT+TGG - chr8_2:21186588-21186607 Msa1239440:CDS 50.0%
GTGATCAGTTCCAACACTGG+AGG + chr8_2:21186601-21186620 None:intergenic 50.0%
! AGGAGTTTTCTCGGAGGATG+TGG - chr8_2:21186671-21186690 Msa1239440:CDS 50.0%
! CGTTGCTGATTGCTGTCATG+AGG - chr8_2:21186709-21186728 Msa1239440:CDS 50.0%
GCTGATTGCTGTCATGAGGA+AGG - chr8_2:21186713-21186732 Msa1239440:CDS 50.0%
CATGAGGAAGGTGACTATGG+CGG - chr8_2:21186725-21186744 Msa1239440:CDS 50.0%
!! CTATGGCGGATCGATATGTG+AGG - chr8_2:21186739-21186758 Msa1239440:CDS 50.0%
GATCCTTGGGATTTCCCTCT+TGG - chr8_2:21186780-21186799 Msa1239440:CDS 50.0%
TCTCGCTACTGGTAAGAACG+AGG + chr8_2:21186963-21186982 None:intergenic 50.0%
! GGCAAGCGAGTTGGAATTGT+TGG - chr8_2:21187283-21187302 Msa1239440:intron 50.0%
AACTTCTAGCGACGCACTTG+TGG - chr8_2:21187432-21187451 Msa1239440:CDS 50.0%
GAAGGAGAAATTGCTGGTGC+TGG - chr8_2:21187592-21187611 Msa1239440:CDS 50.0%
! AAGGTGGAGAAGCATGTTGG+TGG + chr8_2:21186396-21186415 None:intergenic 50.0%
! TCGAAGGTGGAGAAGCATGT+TGG + chr8_2:21186399-21186418 None:intergenic 50.0%
TTCCTAAACCCCAAACCCTC+AGG - chr8_2:21186444-21186463 Msa1239440:CDS 50.0%
AGTGATAGGCCTGAGGGTTT+GGG + chr8_2:21186456-21186475 None:intergenic 50.0%
TGTTGCAGTGATAGGCCTGA+GGG + chr8_2:21186462-21186481 None:intergenic 50.0%
TTGTTGCAGTGATAGGCCTG+AGG + chr8_2:21186463-21186482 None:intergenic 50.0%
TGCAGCGCTACAGAATATGG+CGG + chr8_2:21186523-21186542 None:intergenic 50.0%
GTTGAGAACGTCGGTAGTGA+TGG + chr8_2:21186550-21186569 None:intergenic 50.0%
GGGAAGAAGGTTGAGAACGT+CGG + chr8_2:21186559-21186578 None:intergenic 50.0%
CAACACTGGAGGTGACAACA+AGG + chr8_2:21186590-21186609 None:intergenic 50.0%
! CTTGTTGTCACCTCCAGTGT+TGG - chr8_2:21186588-21186607 Msa1239440:CDS 50.0%
GTGATCAGTTCCAACACTGG+AGG + chr8_2:21186601-21186620 None:intergenic 50.0%
! AGGAGTTTTCTCGGAGGATG+TGG - chr8_2:21186671-21186690 Msa1239440:CDS 50.0%
! CGTTGCTGATTGCTGTCATG+AGG - chr8_2:21186709-21186728 Msa1239440:CDS 50.0%
GCTGATTGCTGTCATGAGGA+AGG - chr8_2:21186713-21186732 Msa1239440:CDS 50.0%
CATGAGGAAGGTGACTATGG+CGG - chr8_2:21186725-21186744 Msa1239440:CDS 50.0%
!! CTATGGCGGATCGATATGTG+AGG - chr8_2:21186739-21186758 Msa1239440:CDS 50.0%
GATCCTTGGGATTTCCCTCT+TGG - chr8_2:21186780-21186799 Msa1239440:CDS 50.0%
TCTCGCTACTGGTAAGAACG+AGG + chr8_2:21186963-21186982 None:intergenic 50.0%
! GGCAAGCGAGTTGGAATTGT+TGG - chr8_2:21187283-21187302 Msa1239440:intron 50.0%
AACTTCTAGCGACGCACTTG+TGG - chr8_2:21187432-21187451 Msa1239440:CDS 50.0%
GAAGGAGAAATTGCTGGTGC+TGG - chr8_2:21187592-21187611 Msa1239440:CDS 50.0%
GTGATAGGCCTGAGGGTTTG+GGG + chr8_2:21186455-21186474 None:intergenic 55.0%
CAGTGATAGGCCTGAGGGTT+TGG + chr8_2:21186457-21186476 None:intergenic 55.0%
GCAGCGCTACAGAATATGGC+GGG + chr8_2:21186522-21186541 None:intergenic 55.0%
GTGACAACAAGGCTGAGCGA+GGG + chr8_2:21186579-21186598 None:intergenic 55.0%
CTGAATGAATGCCGTCGTCG+CGG - chr8_2:21186627-21186646 Msa1239440:CDS 55.0%
CAACCTGAATTCCGCGACGA+CGG + chr8_2:21186641-21186660 None:intergenic 55.0%
CGAGAAAACTCCTCCAGCAC+CGG + chr8_2:21186664-21186683 None:intergenic 55.0%
!! CGGTGCTGGAGGAGTTTTCT+CGG - chr8_2:21186662-21186681 Msa1239440:CDS 55.0%
! TGCTGGAGGAGTTTTCTCGG+AGG - chr8_2:21186665-21186684 Msa1239440:CDS 55.0%
CTCGGAGGATGTGGCTGATA+TGG - chr8_2:21186680-21186699 Msa1239440:CDS 55.0%
GGAGGATGTGGCTGATATGG+CGG - chr8_2:21186683-21186702 Msa1239440:CDS 55.0%
CGGATCGATATGTGAGGACG+CGG - chr8_2:21186745-21186764 Msa1239440:CDS 55.0%
ATCGATATGTGAGGACGCGG+CGG - chr8_2:21186748-21186767 Msa1239440:CDS 55.0%
GCGGGAACGTTCTGATCCTT+GGG - chr8_2:21186767-21186786 Msa1239440:CDS 55.0%
GTGATAGGCCTGAGGGTTTG+GGG + chr8_2:21186455-21186474 None:intergenic 55.0%
CAGTGATAGGCCTGAGGGTT+TGG + chr8_2:21186457-21186476 None:intergenic 55.0%
GCAGCGCTACAGAATATGGC+GGG + chr8_2:21186522-21186541 None:intergenic 55.0%
GTGACAACAAGGCTGAGCGA+GGG + chr8_2:21186579-21186598 None:intergenic 55.0%
CTGAATGAATGCCGTCGTCG+CGG - chr8_2:21186627-21186646 Msa1239440:CDS 55.0%
CAACCTGAATTCCGCGACGA+CGG + chr8_2:21186641-21186660 None:intergenic 55.0%
CGAGAAAACTCCTCCAGCAC+CGG + chr8_2:21186664-21186683 None:intergenic 55.0%
!! CGGTGCTGGAGGAGTTTTCT+CGG - chr8_2:21186662-21186681 Msa1239440:CDS 55.0%
! TGCTGGAGGAGTTTTCTCGG+AGG - chr8_2:21186665-21186684 Msa1239440:CDS 55.0%
CTCGGAGGATGTGGCTGATA+TGG - chr8_2:21186680-21186699 Msa1239440:CDS 55.0%
GGAGGATGTGGCTGATATGG+CGG - chr8_2:21186683-21186702 Msa1239440:CDS 55.0%
CGGATCGATATGTGAGGACG+CGG - chr8_2:21186745-21186764 Msa1239440:CDS 55.0%
ATCGATATGTGAGGACGCGG+CGG - chr8_2:21186748-21186767 Msa1239440:CDS 55.0%
GCGGGAACGTTCTGATCCTT+GGG - chr8_2:21186767-21186786 Msa1239440:CDS 55.0%
! GGCCTGAGGGTTTGGGGTTT+AGG + chr8_2:21186449-21186468 None:intergenic 60.0%
CAAGGCTGAGCGAGGGAAGA+AGG + chr8_2:21186572-21186591 None:intergenic 60.0%
GGTGACAACAAGGCTGAGCG+AGG + chr8_2:21186580-21186599 None:intergenic 60.0%
CGCGACGACGGCATTCATTC+AGG + chr8_2:21186629-21186648 None:intergenic 60.0%
ATGCCGTCGTCGCGGAATTC+AGG - chr8_2:21186635-21186654 Msa1239440:CDS 60.0%
!! CGTCGCGGAATTCAGGTTGC+CGG - chr8_2:21186642-21186661 Msa1239440:CDS 60.0%
!! GGAATTCAGGTTGCCGGTGC+TGG - chr8_2:21186648-21186667 Msa1239440:CDS 60.0%
!! ATTCAGGTTGCCGGTGCTGG+AGG - chr8_2:21186651-21186670 Msa1239440:CDS 60.0%
TCGATATGTGAGGACGCGGC+GGG - chr8_2:21186749-21186768 Msa1239440:CDS 60.0%
GGCGGGAACGTTCTGATCCT+TGG - chr8_2:21186766-21186785 Msa1239440:CDS 60.0%
! GGCCTGAGGGTTTGGGGTTT+AGG + chr8_2:21186449-21186468 None:intergenic 60.0%
CAAGGCTGAGCGAGGGAAGA+AGG + chr8_2:21186572-21186591 None:intergenic 60.0%
GGTGACAACAAGGCTGAGCG+AGG + chr8_2:21186580-21186599 None:intergenic 60.0%
CGCGACGACGGCATTCATTC+AGG + chr8_2:21186629-21186648 None:intergenic 60.0%
ATGCCGTCGTCGCGGAATTC+AGG - chr8_2:21186635-21186654 Msa1239440:CDS 60.0%
!! CGTCGCGGAATTCAGGTTGC+CGG - chr8_2:21186642-21186661 Msa1239440:CDS 60.0%
!! GGAATTCAGGTTGCCGGTGC+TGG - chr8_2:21186648-21186667 Msa1239440:CDS 60.0%
!! ATTCAGGTTGCCGGTGCTGG+AGG - chr8_2:21186651-21186670 Msa1239440:CDS 60.0%
TCGATATGTGAGGACGCGGC+GGG - chr8_2:21186749-21186768 Msa1239440:CDS 60.0%
GGCGGGAACGTTCTGATCCT+TGG - chr8_2:21186766-21186785 Msa1239440:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 21186229 21187888 21186229 ID=Msa1239440;Name=Msa1239440
chr8_2 mRNA 21186229 21187888 21186229 ID=Msa1239440-mRNA-1;Parent=Msa1239440;Name=Msa1239440-mRNA-1;_AED=0.36;_eAED=0.36;_QI=128|1|1|1|1|1|2|84|326
chr8_2 exon 21186229 21186840 21186229 ID=Msa1239440-mRNA-1:exon:7628;Parent=Msa1239440-mRNA-1
chr8_2 exon 21187308 21187888 21187308 ID=Msa1239440-mRNA-1:exon:7627;Parent=Msa1239440-mRNA-1
chr8_2 five_prime_UTR 21187761 21187888 21187761 ID=Msa1239440-mRNA-1:five_prime_utr;Parent=Msa1239440-mRNA-1
chr8_2 CDS 21187308 21187760 21187308 ID=Msa1239440-mRNA-1:cds;Parent=Msa1239440-mRNA-1
chr8_2 CDS 21186313 21186840 21186313 ID=Msa1239440-mRNA-1:cds;Parent=Msa1239440-mRNA-1
chr8_2 three_prime_UTR 21186229 21186312 21186229 ID=Msa1239440-mRNA-1:three_prime_utr;Parent=Msa1239440-mRNA-1
Gene Sequence

>Msa1239440

ATGGCCAAAAACCTTCCAAAAGTACTTGTTCTTGGTCCACCAACATGCTTCTCCACCTTCGAATCACTCTACTCTCACAAATTCAATTTCCTAAACCCCAAACCCTCAGGCCTATCACTGCAACAATTCATCCTTTCCCACAACCACAATCCATCAACAATTCCCGCCATATTCTGTAGCGCTGCATATTCCATCACTACCGACGTTCTCAACCTTCTTCCCTCGCTCAGCCTTGTTGTCACCTCCAGTGTTGGAACTGATCACATTGACCTGAATGAATGCCGTCGTCGCGGAATTCAGGTTGCCGGTGCTGGAGGAGTTTTCTCGGAGGATGTGGCTGATATGGCGGTTGCGTTGCTGATTGCTGTCATGAGGAAGGTGACTATGGCGGATCGATATGTGAGGACGCGGCGGGAACGTTCTGATCCTTGGGATTTCCCTCTTGGATACAAGTTATCAGGCAAGCGAGTTGGAATTGTTGGATTAGGAAGTATTGGCATGGAAGTAGCTAAGCGACTCGAGTCATTTGATTGCATTATCTCGTACCACTCTAAGCATCAAAAGACATCAATTTCGTACCCTTTCTATTCCAATGTTATTGATCTTGCAACTTCTAGCGACGCACTTGTGGTATGTTGTGCACTAAACAACCAAACACGACACATTATAAACAACGATGTCCTTTTGGCATTAGGAAAAGAAGGGTTTATTGTAAATGTTGGAAGAGGTGGTCTTATTGATGAGAAACAATTGATCAAGTGTTTGATGGAAGGAGAAATTGCTGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATGTTGATGAAAAGCTCTTTGCAATGGATAATGTGGTTTTATCTCCACATTCTGCAGCTTTTACTGTTGAAGCTACAATGAGTTTGTGTGAACTTGTGGCAGGGAATTTAGAAGCTTTCTTCTTAAACAAGCCACTAATTAGTCCAGTGAAGTTGGTTGAGTAA
Protein sequence

>Msa1239440

MAKNLPKVLVLGPPTCFSTFESLYSHKFNFLNPKPSGLSLQQFILSHNHNPSTIPAIFCSAAYSITTDVLNLLPSLSLVVTSSVGTDHIDLNECRRRGIQVAGAGGVFSEDVADMAVALLIAVMRKVTMADRYVRTRRERSDPWDFPLGYKLSGKRVGIVGLGSIGMEVAKRLESFDCIISYHSKHQKTSISYPFYSNVIDLATSSDALVVCCALNNQTRHIINNDVLLALGKEGFIVNVGRGGLIDEKQLIKCLMEGEIAGAGLDVFENEPHVDEKLFAMDNVVLSPHSAAFTVEATMSLCELVAGNLEAFFLNKPLISPVKLVE*