AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0694910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694910 MtrunA17_Chr4g0045941 93.017 358 14 3 1 353 1 352 0.0 678
Msa0694910 MtrunA17_Chr4g0045951 80.811 370 48 4 1 353 1 364 0.0 598
Msa0694910 MtrunA17_Chr2g0283581 68.994 358 99 4 1 353 10 360 1.25e-179 502
Msa0694910 MtrunA17_Chr2g0325481 59.271 329 120 5 30 352 47 367 1.37e-140 403
Msa0694910 MtrunA17_Chr4g0041011 57.187 327 128 4 30 351 44 363 2.58e-132 382
Msa0694910 MtrunA17_Chr3g0097891 52.408 353 154 6 8 352 5 351 2.61e-129 374
Msa0694910 MtrunA17_Chr2g0314801 53.681 326 137 6 30 347 30 349 2.85e-120 351
Msa0694910 MtrunA17_Chr3g0085081 50.145 345 156 7 16 351 11 348 5.80e-118 345
Msa0694910 MtrunA17_Chr3g0085071 50.305 328 150 5 30 351 43 363 3.41e-117 343
Msa0694910 MtrunA17_Chr3g0091031 47.619 357 162 7 1 351 11 348 2.60e-116 341
Msa0694910 MtrunA17_Chr5g0413851 49.857 349 155 7 16 352 20 360 3.32e-116 341
Msa0694910 MtrunA17_Chr3g0105301 47.863 351 156 8 23 352 33 377 9.49e-110 325
Msa0694910 MtrunA17_Chr5g0430371 48.209 363 149 9 23 352 44 400 2.33e-109 325
Msa0694910 MtrunA17_Chr2g0314791 44.505 364 172 9 1 352 1 346 5.38e-102 304
Msa0694910 MtrunA17_Chr1g0171171 40.356 337 175 6 30 351 50 375 9.90e-87 266
Msa0694910 MtrunA17_Chr1g0175671 38.728 346 183 8 33 353 170 511 4.73e-86 269
Msa0694910 MtrunA17_Chr7g0258851 39.884 346 179 7 33 353 248 589 4.04e-84 266
Msa0694910 MtrunA17_Chr4g0068111 38.120 383 196 11 7 353 78 455 6.22e-83 259
Msa0694910 MtrunA17_Chr1g0210771 36.364 352 189 8 20 351 46 382 1.36e-80 251
Msa0694910 MtrunA17_Chr4g0071391 37.101 345 188 7 33 353 109 448 4.99e-78 246
Msa0694910 MtrunA17_Chr1g0213281 37.101 345 193 8 31 352 173 516 4.77e-76 243
Msa0694910 MtrunA17_Chr3g0113671 37.607 351 184 10 32 352 105 450 1.31e-75 240
Msa0694910 MtrunA17_Chr5g0413271 36.283 339 191 7 33 352 145 477 1.40e-74 238
Msa0694910 MtrunA17_Chr8g0368461 35.657 373 200 10 19 352 158 529 2.57e-74 239
Msa0694910 MtrunA17_Chr4g0006991 37.176 347 185 9 33 351 86 427 4.11e-74 235
Msa0694910 MtrunA17_Chr5g0437291 38.136 354 181 11 32 352 164 512 2.82e-73 236
Msa0694910 MtrunA17_Chr7g0269991 35.754 358 200 9 11 347 115 463 7.19e-71 228
Msa0694910 MtrunA17_Chr4g0005491 34.551 356 200 9 27 352 56 408 1.32e-69 223
Msa0694910 MtrunA17_Chr8g0360521 35.733 375 207 12 5 352 43 410 1.74e-69 223
Msa0694910 MtrunA17_Chr3g0114101 35.345 348 192 10 33 352 89 431 8.14e-69 222
Msa0694910 MtrunA17_Chr1g0209921 45.690 232 116 6 30 256 41 267 2.41e-68 215
Msa0694910 MtrunA17_Chr7g0243301 35.411 353 193 11 30 351 61 409 6.33e-67 216
Msa0694910 MtrunA17_Chr5g0437601 34.270 356 201 11 25 352 75 425 1.33e-65 213
Msa0694910 MtrunA17_Chr7g0242701 34.857 350 195 9 30 351 83 427 3.40e-65 212
Msa0694910 MtrunA17_Chr7g0259711 35.127 353 198 10 27 352 85 433 7.30e-65 212
Msa0694910 MtrunA17_Chr3g0126021 36.963 349 194 8 25 352 117 460 1.67e-64 211
Msa0694910 MtrunA17_Chr2g0287901 34.181 354 197 11 28 352 92 438 3.92e-63 207
Msa0694910 MtrunA17_Chr4g0005481 34.384 349 195 12 26 347 101 442 1.09e-62 206
Msa0694910 MtrunA17_Chr8g0384151 34.431 334 199 7 33 352 90 417 1.17e-62 206
Msa0694910 MtrunA17_Chr1g0178081 32.075 371 176 13 3 352 27 342 6.45e-48 165
Msa0694910 MtrunA17_Chr5g0414061 29.429 350 209 10 30 347 91 434 1.02e-37 140
Msa0694910 MtrunA17_Chr6g0456681 30.110 362 221 10 15 347 46 404 1.65e-37 139
Msa0694910 MtrunA17_Chr6g0456691 29.345 351 213 8 32 353 77 421 1.67e-36 137
Msa0694910 MtrunA17_Chr5g0414091 31.034 348 198 11 32 344 84 424 3.20e-36 136
Msa0694910 MtrunA17_Chr3g0085101 55.660 106 46 1 218 322 1 106 4.22e-34 122
Msa0694910 MtrunA17_Chr5g0414051 29.745 353 199 15 33 347 75 416 6.25e-34 129
Msa0694910 MtrunA17_Chr6g0476041 28.165 387 189 15 20 347 154 510 1.94e-33 129
Msa0694910 MtrunA17_Chr8g0384421 30.682 352 207 15 29 351 129 472 3.96e-32 125
Msa0694910 MtrunA17_Chr4g0049421 28.490 351 215 14 33 352 48 393 1.03e-31 123
Msa0694910 MtrunA17_Chr4g0070341 30.278 360 214 14 24 351 117 471 2.28e-31 123
Msa0694910 MtrunA17_Chr8g0347321 26.761 355 216 11 33 352 95 440 3.45e-31 122
Msa0694910 MtrunA17_Chr5g0414041 28.080 349 211 12 33 347 87 429 3.73e-30 119
Msa0694910 MtrunA17_Chr1g0188191 27.320 388 217 16 12 347 203 577 4.29e-30 120
Msa0694910 MtrunA17_Chr2g0283851 28.011 357 223 12 22 347 43 396 3.17e-29 116
Msa0694910 MtrunA17_Chr3g0140371 26.346 353 212 13 31 347 16 356 5.45e-29 115
Msa0694910 MtrunA17_Chr8g0387921 26.816 358 219 11 30 352 71 420 2.21e-28 114
Msa0694910 MtrunA17_Chr8g0391481 27.174 368 218 13 21 347 10 368 1.40e-27 111
Msa0694910 MtrunA17_Chr7g0226991 27.684 354 205 13 33 347 57 398 2.49e-27 111
Msa0694910 MtrunA17_Chr6g0451321 27.493 371 204 16 24 351 109 457 1.97e-26 109
Msa0694910 MtrunA17_Chr7g0221271 35.606 132 80 1 33 164 79 205 1.62e-22 94.0
Msa0694910 MtrunA17_Chr6g0455901 25.895 363 208 13 29 347 67 412 2.74e-22 97.1
Msa0694910 MtrunA17_Chr1g0209931 50.562 89 40 2 265 352 8 93 6.44e-21 86.3
Msa0694910 MtrunA17_Chr7g0221291 35.185 108 70 0 14 121 60 167 3.93e-19 83.6
Msa0694910 MtrunA17_Chr5g0429211 47.727 88 40 2 79 166 187 268 1.63e-17 81.6
Msa0694910 MtrunA17_Chr2g0287911 46.774 62 26 1 27 88 55 109 5.95e-13 64.7
Msa0694910 MtrunA17_Chr2g0287931 29.688 128 76 5 93 209 2 126 1.39e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694910 AT1G29050.1 63.127 339 112 5 21 353 49 380 4.65e-162 458
Msa0694910 AT2G34070.1 62.763 333 110 5 28 353 60 385 1.88e-156 444
Msa0694910 AT2G31110.2 53.315 362 150 4 3 351 7 362 2.26e-140 403
Msa0694910 AT2G42570.1 56.928 332 132 3 26 352 40 365 1.29e-138 398
Msa0694910 AT3G14850.2 52.280 329 141 6 28 347 29 350 4.13e-118 346
Msa0694910 AT2G30900.1 50.152 329 150 6 33 352 43 366 1.10e-114 338
Msa0694910 AT1G78710.1 47.289 332 161 5 28 352 33 357 7.08e-114 335
Msa0694910 AT2G34070.2 63.566 258 80 5 103 353 1 251 1.01e-112 328
Msa0694910 AT5G58600.1 49.128 344 147 9 31 352 64 401 3.71e-106 317
Msa0694910 AT2G30010.1 45.093 377 170 9 8 352 26 397 1.29e-100 303
Msa0694910 AT2G30010.2 45.093 377 170 9 8 352 69 440 1.93e-100 304
Msa0694910 AT2G31110.3 51.341 261 108 4 3 250 7 261 3.75e-91 274
Msa0694910 AT2G31110.1 58.173 208 81 2 149 351 8 214 3.52e-86 259
Msa0694910 AT1G78710.2 40.673 327 137 5 28 352 26 297 3.59e-86 262
Msa0694910 AT3G14850.1 52.756 254 104 6 103 347 1 247 9.28e-86 259
Msa0694910 AT5G06700.1 39.542 349 178 8 32 353 254 596 5.37e-84 266
Msa0694910 AT3G54260.1 39.003 341 171 9 33 352 51 375 4.48e-82 254
Msa0694910 AT3G12060.1 39.255 349 179 8 32 353 195 537 2.73e-80 255
Msa0694910 AT5G49340.1 35.849 371 213 7 7 353 74 443 3.14e-80 252
Msa0694910 AT5G01360.2 37.143 350 186 8 31 351 34 378 2.35e-79 248
Msa0694910 AT5G20590.1 37.288 354 200 7 21 353 129 481 2.84e-79 251
Msa0694910 AT5G01360.1 37.143 350 186 8 31 351 90 434 7.31e-79 248
Msa0694910 AT2G30900.2 50.216 231 104 4 33 257 43 268 2.23e-77 239
Msa0694910 AT5G58600.2 50.427 234 103 6 31 257 64 291 6.17e-74 231
Msa0694910 AT1G60790.1 36.416 346 194 8 32 352 189 533 3.27e-72 234
Msa0694910 AT1G73140.1 36.752 351 190 10 28 352 62 406 5.07e-72 230
Msa0694910 AT3G62390.1 37.101 345 192 9 28 352 133 472 3.84e-71 229
Msa0694910 AT2G40150.1 35.754 358 189 11 31 352 69 421 1.38e-69 224
Msa0694910 AT3G55990.1 36.364 352 189 11 31 352 138 484 2.66e-69 225
Msa0694910 AT3G11030.1 36.313 358 192 12 25 352 98 449 2.96e-68 221
Msa0694910 AT5G19160.1 35.635 362 186 13 28 352 94 445 5.57e-68 221
Msa0694910 AT1G48880.1 36.228 334 181 8 33 347 115 435 5.81e-67 218
Msa0694910 AT2G40320.1 36.134 357 193 11 25 352 73 423 2.20e-66 216
Msa0694910 AT5G06230.2 34.670 349 201 9 30 352 18 365 7.39e-66 213
Msa0694910 AT5G06230.1 33.333 372 221 9 7 352 36 406 8.38e-66 214
Msa0694910 AT2G40160.1 34.444 360 184 12 33 352 77 424 2.64e-65 213
Msa0694910 AT3G06080.2 34.857 350 202 8 28 352 98 446 4.11e-65 213
Msa0694910 AT2G40160.2 34.444 360 184 12 33 352 77 424 4.58e-65 213
Msa0694910 AT5G01620.2 34.091 352 200 12 23 347 99 445 1.78e-64 211
Msa0694910 AT5G01620.1 34.091 352 200 12 23 347 99 445 1.78e-64 211
Msa0694910 AT2G38320.1 34.000 350 198 10 33 352 59 405 1.81e-64 210
Msa0694910 AT5G01620.3 34.091 352 200 12 23 347 107 453 2.64e-64 211
Msa0694910 AT3G11570.1 34.568 324 191 8 49 352 98 420 2.13e-60 200
Msa0694910 AT2G38320.2 32.298 322 186 9 60 352 4 322 8.56e-54 180
Msa0694910 AT5G20590.2 35.887 248 146 5 21 255 129 376 1.63e-49 170
Msa0694910 AT5G19160.2 35.366 246 136 7 28 255 94 334 3.45e-49 169
Msa0694910 AT3G62390.2 34.711 242 141 6 28 255 133 371 3.13e-45 160
Msa0694910 AT2G40160.3 32.917 240 138 6 33 255 77 310 1.30e-43 153
Msa0694910 AT3G06080.1 33.610 241 147 5 28 255 98 338 1.83e-42 151
Msa0694910 AT5G06230.3 30.741 270 168 6 7 257 36 305 3.59e-40 144
Msa0694910 AT1G70230.1 29.032 341 204 13 33 347 79 407 2.33e-36 136
Msa0694910 AT4G25360.1 29.945 364 200 14 27 347 169 520 2.10e-35 135
Msa0694910 AT4G25360.2 29.945 364 200 14 27 347 169 520 2.10e-35 135
Msa0694910 AT5G64470.2 30.168 358 202 15 33 353 55 401 1.61e-34 131
Msa0694910 AT5G64470.3 29.060 351 209 13 33 353 55 395 2.05e-34 130
Msa0694910 AT5G64020.1 29.363 361 204 13 33 351 57 408 4.41e-34 130
Msa0694910 AT5G51640.1 28.792 389 203 13 12 347 117 484 5.55e-34 131
Msa0694910 AT2G37720.1 29.132 357 198 14 33 351 143 482 3.28e-33 129
Msa0694910 AT1G01430.1 27.049 366 221 9 25 352 91 448 6.03e-33 127
Msa0694910 AT5G15890.1 26.923 364 214 10 20 347 171 518 1.26e-30 122
Msa0694910 AT5G15900.1 26.776 366 234 8 15 351 49 409 1.37e-30 120
Msa0694910 AT5G15900.2 26.776 366 234 8 15 351 68 428 1.62e-30 120
Msa0694910 AT3G02440.1 26.225 347 215 9 33 347 127 464 2.12e-29 118
Msa0694910 AT3G02440.2 26.225 347 215 9 33 347 135 472 2.40e-29 118
Msa0694910 AT5G20680.6 28.736 348 211 16 33 351 118 457 6.05e-29 116
Msa0694910 AT5G20680.4 28.736 348 211 16 33 351 212 551 1.21e-28 117
Msa0694910 AT5G20680.1 28.736 348 211 16 33 351 212 551 1.21e-28 117
Msa0694910 AT5G20680.3 28.736 348 211 16 33 351 212 551 1.21e-28 117
Msa0694910 AT5G20680.5 28.736 348 211 16 33 351 212 551 1.21e-28 117
Msa0694910 AT5G20680.2 28.736 348 211 16 33 351 194 533 1.47e-28 116
Msa0694910 AT3G28150.1 28.101 395 212 15 2 351 43 410 1.78e-28 115
Msa0694910 AT4G01080.1 27.887 355 203 12 30 347 88 426 2.39e-28 115
Msa0694910 AT4G11090.1 28.571 357 199 14 33 347 79 421 2.78e-27 112
Msa0694910 AT2G14530.1 29.692 357 207 14 30 351 59 406 5.16e-27 110
Msa0694910 AT4G23790.1 26.685 356 219 11 33 352 79 428 3.85e-26 108
Msa0694910 AT5G64470.4 26.772 254 155 8 33 263 55 300 1.11e-18 85.9
Msa0694910 AT5G64470.1 26.772 254 155 8 33 263 55 300 1.13e-18 85.9

Find 91 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATTGTAATTTGTTCTT+TGG 0.220399 4_4:+64300821 Msa0694910:CDS
ACTATACCGTACACCATATT+TGG 0.252473 4_4:+64303118 Msa0694910:CDS
AATTTAATTACTATATATAT+TGG 0.269310 4_4:+64304199 Msa0694910:three_prime_UTR
ATTATTATATCCACCAAATA+TGG 0.271330 4_4:-64303131 None:intergenic
TATTTAAGATACTGATTATC+AGG 0.276183 4_4:-64300935 None:intergenic
ACATGATTAGAGCTGCATTT+AGG 0.303593 4_4:-64304098 None:intergenic
TTGTTCACCACAACATTTGC+AGG 0.326743 4_4:-64303916 None:intergenic
AGGAGTATGTTCCAAGTATC+AGG 0.327240 4_4:-64304078 None:intergenic
TTGCTGGGTGCATGGATATA+AGG 0.329977 4_4:-64300784 None:intergenic
CAATGTCTACTGTCTTCTTC+CGG 0.356385 4_4:+64301385 Msa0694910:CDS
AAAACTAAACTCTTCTTTCA+AGG 0.357639 4_4:+64303646 Msa0694910:CDS
GCATGGATATAAGGAGATTT+AGG 0.362396 4_4:-64300775 None:intergenic
CGGGGAAGTTGAACCATTAC+CGG 0.374830 4_4:+64303867 Msa0694910:CDS
AGATTGCATGCACAGGACTA+TGG 0.376697 4_4:+64303083 Msa0694910:CDS
GAAAAGCTTGTAGTGACATT+AGG 0.381141 4_4:-64301351 None:intergenic
ATCAGTATCTTAAATACTCT+TGG 0.387106 4_4:+64300942 Msa0694910:CDS
GGATATGTGGTTCCCGGTAA+TGG 0.391578 4_4:-64303880 None:intergenic
CTGGGTAAAGCACATGAATC+AGG 0.414998 4_4:-64300968 None:intergenic
TGTGAAATTAGAGTATATAT+TGG 0.416927 4_4:+64304118 Msa0694910:exon
ATTGACCACTTGGGCTGGAT+GGG 0.420395 4_4:+64303600 Msa0694910:CDS
AATGTCTACTGTCTTCTTCC+GGG 0.421225 4_4:+64301386 Msa0694910:CDS
AGTGAGGGACATGGACCGTT+TGG 0.425551 4_4:+64303561 Msa0694910:CDS
TCCCCGCTACATGTCTTCTT+CGG 0.430209 4_4:-64303850 None:intergenic
GATAAGAAGATAATGTTTGT+AGG 0.445963 4_4:+64301270 Msa0694910:CDS
TTGAATCCAGAGGGCAGGAT+TGG 0.450495 4_4:+64303821 Msa0694910:intron
TTACTTACTTGTTATGAGTA+GGG 0.455719 4_4:-64303675 None:intergenic
GTAATTTGTTCTTTGGAAGC+TGG 0.456024 4_4:+64300828 Msa0694910:CDS
TTTATAAGGGATTGACCACT+TGG 0.465985 4_4:+64303590 Msa0694910:CDS
TCTTCAACTCCTGGCATTGG+TGG 0.467165 4_4:+64303231 Msa0694910:CDS
TGTAGTCATTGGTGCCTACC+AGG 0.474740 4_4:+64304051 Msa0694910:CDS
AAGGGATTGACCACTTGGGC+TGG 0.475375 4_4:+64303595 Msa0694910:CDS
TTGTGCTCTTGCTGGGTGCA+TGG 0.478358 4_4:-64300792 None:intergenic
TACCAGGACTACCTGATACT+TGG 0.478567 4_4:+64304067 Msa0694910:CDS
TAATTTGTTCTTTGGAAGCT+GGG 0.484946 4_4:+64300829 Msa0694910:CDS
GATTGACCACTTGGGCTGGA+TGG 0.486358 4_4:+64303599 Msa0694910:CDS
TGGTGTACGGTATAGTTGTA+TGG 0.491607 4_4:-64303111 None:intergenic
GGATCCTTAGTATAGATAGA+AGG 0.493596 4_4:-64304015 None:intergenic
ATAATAATTCAAGAAGATGT+TGG 0.497836 4_4:+64303146 Msa0694910:CDS
TGATCTTCAACTCCTGGCAT+TGG 0.498503 4_4:+64303228 Msa0694910:CDS
GTGGTGTAGGTTCAGTGGAG+AGG 0.499875 4_4:+64301227 Msa0694910:intron
GTTAAAGAACATGAAGAAGC+AGG 0.504244 4_4:+64303942 Msa0694910:CDS
ATCTATACTAAGGATCCAAC+TGG 0.504484 4_4:+64304021 Msa0694910:CDS
CTGGCATTGATTGTAGTCAT+TGG 0.505796 4_4:+64304040 Msa0694910:CDS
ATTACCGGGAACCACATATC+CGG 0.506278 4_4:+64303882 Msa0694910:CDS
GTCTTGTATGATTCATGCGT+CGG 0.512224 4_4:+64301326 Msa0694910:CDS
ATCAAATTGAGCTTCAACGA+AGG 0.515709 4_4:-64300894 None:intergenic
GTTACTTACTTGTTATGAGT+AGG 0.517658 4_4:-64303676 None:intergenic
TCTCCTTGAATCCAGAGGGC+AGG 0.517922 4_4:+64303816 Msa0694910:intron
ACAACATTTGCAGGAGGTAG+AGG 0.519041 4_4:-64303907 None:intergenic
ACATCCTTCTATCTATACTA+AGG 0.519367 4_4:+64304011 Msa0694910:CDS
TATGGACATTGAAAGCCATA+TGG 0.524499 4_4:-64300727 None:intergenic
AGGTAGAGGGGCCGGATATG+TGG 0.530211 4_4:-64303893 None:intergenic
TACTTACTTGTTATGAGTAG+GGG 0.532035 4_4:-64303674 None:intergenic
TTCCAAGTATCAGGTAGTCC+TGG 0.535659 4_4:-64304069 None:intergenic
AACCGAAGAAGACATGTAGC+GGG 0.541404 4_4:+64303848 Msa0694910:CDS
ACATGTTGATCTTCAACTCC+TGG 0.546418 4_4:+64303222 Msa0694910:CDS
AGTGTGGATCCACCAATGCC+AGG 0.547954 4_4:-64303240 None:intergenic
TTTGCAGGAGGTAGAGGGGC+CGG 0.548515 4_4:-64303901 None:intergenic
CAACATTTGCAGGAGGTAGA+GGG 0.554553 4_4:-64303906 None:intergenic
GGGGCCGGATATGTGGTTCC+CGG 0.554885 4_4:-64303886 None:intergenic
TGACTACAATCAATGCCAGT+TGG 0.558982 4_4:-64304036 None:intergenic
ATACCGTACACCATATTTGG+TGG 0.560969 4_4:+64303121 Msa0694910:CDS
CCTTATAAAATGCATCCAAA+CGG 0.562648 4_4:-64303576 None:intergenic
CTGATTCATGTGCTTTACCC+AGG 0.569924 4_4:+64300969 Msa0694910:CDS
TGGCATTGGTGGATCCACAC+TGG 0.570664 4_4:+64303242 Msa0694910:CDS
ACGCTGGACTCTATAAAAGC+TGG 0.571735 4_4:+64303179 Msa0694910:CDS
ATTCATTGAGTCTAAACATG+TGG 0.578501 4_4:+64301295 Msa0694910:CDS
TCTACCTCCTGCAAATGTTG+TGG 0.589800 4_4:+64303909 Msa0694910:CDS
TATCCACCAAATATGGTGTA+CGG 0.590876 4_4:-64303124 None:intergenic
CTATAAAAGCTGGTAAAGCA+TGG 0.592608 4_4:+64303189 Msa0694910:CDS
GGGGAAGTTGAACCATTACC+GGG 0.597940 4_4:+64303868 Msa0694910:CDS
TCAAATTGAGCTTCAACGAA+GGG 0.606381 4_4:-64300893 None:intergenic
TTCATTGAGTCTAAACATGT+GGG 0.609808 4_4:+64301296 Msa0694910:CDS
CTTGGAGAGATTGCATGCAC+AGG 0.610204 4_4:+64303076 Msa0694910:intron
GAGATGGTTCTAATCTAGTG+AGG 0.627215 4_4:+64303545 Msa0694910:intron
AAGTATCAGGTAGTCCTGGT+AGG 0.628734 4_4:-64304065 None:intergenic
ATCAACCCATCCAGCCCAAG+TGG 0.629219 4_4:-64303605 None:intergenic
AACATTTGCAGGAGGTAGAG+GGG 0.630305 4_4:-64303905 None:intergenic
AGATGGTTCTAATCTAGTGA+GGG 0.631565 4_4:+64303546 Msa0694910:CDS
TGTTGGTAGAGTGCTTACGC+TGG 0.633724 4_4:+64303163 Msa0694910:CDS
TTATAAGGGATTGACCACTT+GGG 0.640369 4_4:+64303591 Msa0694910:CDS
GAACCGAAGAAGACATGTAG+CGG 0.645973 4_4:+64303847 Msa0694910:CDS
CGGAAGAAGACAGTAGACAT+TGG 0.646035 4_4:-64301384 None:intergenic
CAGCTTGAAGAATCATAGAG+AGG 0.652645 4_4:-64300869 None:intergenic
TTCTAATCTAGTGAGGGACA+TGG 0.659068 4_4:+64303552 Msa0694910:CDS
TTCACCACAACATTTGCAGG+AGG 0.663611 4_4:-64303913 None:intergenic
TGGTAAAGCATGGTTAGACA+TGG 0.665553 4_4:+64303199 Msa0694910:CDS
ATCCACACTGGAGATTTACG+AGG 0.690791 4_4:+64303254 Msa0694910:CDS
ACCGAAGAAGACATGTAGCG+GGG 0.713042 4_4:+64303849 Msa0694910:CDS
TCCACACTGGAGATTTACGA+GGG 0.713392 4_4:+64303255 Msa0694910:CDS
ACCCTCGTAAATCTCCAGTG+TGG 0.727033 4_4:-64303256 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAATAATATTAAAGTG+AGG + chr4_4:64302040-64302059 Msa0694910:intron 10.0%
!! AAAAAAAAAATCAAATATGA+AGG - chr4_4:64302813-64302832 None:intergenic 10.0%
!!! ATCATTGATTTTTTTTTTTA+AGG - chr4_4:64302989-64303008 None:intergenic 10.0%
!!! AATAATTGTAATTTGTTCTT+TGG + chr4_4:64300821-64300840 Msa0694910:CDS 15.0%
!! AACTATATATATATATAGGT+CGG - chr4_4:64302006-64302025 None:intergenic 15.0%
!!! AAGACTTATTATTTTAAGAT+TGG - chr4_4:64302275-64302294 None:intergenic 15.0%
!! CATTAATTCATTATAATAGA+TGG + chr4_4:64302751-64302770 Msa0694910:intron 15.0%
!! ATAAAGTAAAGATTCAATTT+GGG - chr4_4:64302874-64302893 None:intergenic 15.0%
!! AATAAAGTAAAGATTCAATT+TGG - chr4_4:64302875-64302894 None:intergenic 15.0%
!!! GATAAAAATATCCATATTTT+TGG + chr4_4:64302912-64302931 Msa0694910:intron 15.0%
!! ATCATTGATATTATCATATT+GGG - chr4_4:64302957-64302976 None:intergenic 15.0%
!! TATCATTGATATTATCATAT+TGG - chr4_4:64302958-64302977 None:intergenic 15.0%
!!! TATTTAAGATACTGATTATC+AGG - chr4_4:64300938-64300957 None:intergenic 20.0%
!! TGTATGTGACAATTAATATT+TGG + chr4_4:64301143-64301162 Msa0694910:intron 20.0%
!! TTACTCATTACATATACATT+AGG - chr4_4:64301199-64301218 None:intergenic 20.0%
!!! GTAATGAGTAATTATTTTTG+TGG + chr4_4:64301208-64301227 Msa0694910:intron 20.0%
!!! AATATTTCTATGGAAAATGA+TGG - chr4_4:64301674-64301693 None:intergenic 20.0%
!! AATTAACTCGTTGAATAAAA+TGG + chr4_4:64301757-64301776 Msa0694910:intron 20.0%
!! AATATCTAGATTTAGTGATA+AGG - chr4_4:64302133-64302152 None:intergenic 20.0%
!!! ATTGGACCATAATTTTAAAA+AGG + chr4_4:64302244-64302263 Msa0694910:intron 20.0%
!!! GTTTGACCTTTTTAAAATTA+TGG - chr4_4:64302253-64302272 None:intergenic 20.0%
!!! TACTTTATTTCTATCCATAT+AGG + chr4_4:64302886-64302905 Msa0694910:intron 20.0%
!!! ACTTTATTTCTATCCATATA+GGG + chr4_4:64302887-64302906 Msa0694910:intron 20.0%
!! TCATTGATATTATCATATTG+GGG - chr4_4:64302956-64302975 None:intergenic 20.0%
!! ATTATTATATCCACCAAATA+TGG - chr4_4:64303134-64303153 None:intergenic 20.0%
!! ATAATAATTCAAGAAGATGT+TGG + chr4_4:64303146-64303165 Msa0694910:CDS 20.0%
!! AGTCTACAATAATACTATTT+AGG + chr4_4:64303286-64303305 Msa0694910:intron 20.0%
!! TTCAAATATACATTCAATTG+TGG - chr4_4:64303333-64303352 None:intergenic 20.0%
!! TTGAATGTATATTTGAATTG+AGG + chr4_4:64303336-64303355 Msa0694910:intron 20.0%
!! TGTGAAATTAGAGTATATAT+TGG + chr4_4:64304118-64304137 Msa0694910:exon 20.0%
! CAATTTGATTGCCAAAAATA+TGG + chr4_4:64300908-64300927 Msa0694910:CDS 25.0%
! ATCAGTATCTTAAATACTCT+TGG + chr4_4:64300942-64300961 Msa0694910:CDS 25.0%
! TATATGTGACATATCACAAA+GGG - chr4_4:64301033-64301052 None:intergenic 25.0%
! TTATATGTGACATATCACAA+AGG - chr4_4:64301034-64301053 None:intergenic 25.0%
!!! ATAAACGTGTTTTGTTTCTA+TGG - chr4_4:64301114-64301133 None:intergenic 25.0%
!!! AGTAATTATTTTTGTGGTGT+AGG + chr4_4:64301214-64301233 Msa0694910:intron 25.0%
! GATAAGAAGATAATGTTTGT+AGG + chr4_4:64301270-64301289 Msa0694910:CDS 25.0%
!!! TCATTTTGTTTGTTTCTTTG+CGG + chr4_4:64301486-64301505 Msa0694910:intron 25.0%
! AATATCGAGCTTAATATCTT+TGG - chr4_4:64301643-64301662 None:intergenic 25.0%
!! GTCTCATTCAAATATTTCTA+TGG - chr4_4:64301684-64301703 None:intergenic 25.0%
! AGAAATATTTGAATGAGACT+AGG + chr4_4:64301685-64301704 Msa0694910:intron 25.0%
! TAAAGAAGCTAGTAATAGAT+TGG + chr4_4:64302226-64302245 Msa0694910:intron 25.0%
!! CAATCAGACTTTGTTTAAAT+AGG - chr4_4:64302354-64302373 None:intergenic 25.0%
! CTATTTAAACAAAGTCTGAT+TGG + chr4_4:64302352-64302371 Msa0694910:intron 25.0%
!! TTTAGAGTTGTCTTTACATA+AGG - chr4_4:64302524-64302543 None:intergenic 25.0%
! TAAATTTGCATTGTCATTGT+AGG + chr4_4:64302621-64302640 Msa0694910:intron 25.0%
! AAATTTGCATTGTCATTGTA+GGG + chr4_4:64302622-64302641 Msa0694910:intron 25.0%
! AGGAAGATAAAAAATTGCAA+GGG - chr4_4:64302701-64302720 None:intergenic 25.0%
! AAGGAAGATAAAAAATTGCA+AGG - chr4_4:64302702-64302721 None:intergenic 25.0%
!!! ATATTTTTATCCACCCTATA+TGG - chr4_4:64302903-64302922 None:intergenic 25.0%
!!! TATCCATATTTTTGGATATC+CGG + chr4_4:64302920-64302939 Msa0694910:intron 25.0%
! CACTAACACAGTTTAATTAT+GGG - chr4_4:64303053-64303072 None:intergenic 25.0%
! TCACTAACACAGTTTAATTA+TGG - chr4_4:64303054-64303073 None:intergenic 25.0%
! GGTACTACATAATAAATAGT+AGG - chr4_4:64303312-64303331 None:intergenic 25.0%
!! AAAGTGATGAATGTGTTATT+TGG + chr4_4:64303494-64303513 Msa0694910:intron 25.0%
!!! AGAAGAGTTTAGTTTTAGTA+GGG - chr4_4:64303642-64303661 None:intergenic 25.0%
!!! AAGAAGAGTTTAGTTTTAGT+AGG - chr4_4:64303643-64303662 None:intergenic 25.0%
! AAAACTAAACTCTTCTTTCA+AGG + chr4_4:64303646-64303665 Msa0694910:CDS 25.0%
!!! TTACTTACTTGTTATGAGTA+GGG - chr4_4:64303678-64303697 None:intergenic 25.0%
!! CTCTAATTAAAAGTACGTAT+TGG - chr4_4:64303723-64303742 None:intergenic 25.0%
!! TTTTCGTAAATGTTCTTAAG+AGG + chr4_4:64304230-64304249 Msa0694910:three_prime_UTR 25.0%
!! TAATTTGTTCTTTGGAAGCT+GGG + chr4_4:64300829-64300848 Msa0694910:CDS 30.0%
! TATCAGGTCTTCCATATTTT+TGG - chr4_4:64300922-64300941 None:intergenic 30.0%
TACAAACTAACAGTATACCT+GGG - chr4_4:64300989-64301008 None:intergenic 30.0%
ATACAAACTAACAGTATACC+TGG - chr4_4:64300990-64301009 None:intergenic 30.0%
CAAAGATCAAACACTATAGT+AGG - chr4_4:64301071-64301090 None:intergenic 30.0%
ATTCATTGAGTCTAAACATG+TGG + chr4_4:64301295-64301314 Msa0694910:CDS 30.0%
TTCATTGAGTCTAAACATGT+GGG + chr4_4:64301296-64301315 Msa0694910:CDS 30.0%
AAACAAGAACAACTATTACC+CGG - chr4_4:64301407-64301426 None:intergenic 30.0%
!! CATTTTGTTTGTTTCTTTGC+GGG + chr4_4:64301487-64301506 Msa0694910:intron 30.0%
! CCTATCATTTTACATGTAAC+AGG - chr4_4:64301591-64301610 None:intergenic 30.0%
CCTGTTACATGTAAAATGAT+AGG + chr4_4:64301588-64301607 Msa0694910:intron 30.0%
TATTTGAATGAGACTAGGTT+AGG + chr4_4:64301690-64301709 Msa0694910:intron 30.0%
TAGTTGATCTACATAAAGTC+TGG + chr4_4:64301731-64301750 Msa0694910:intron 30.0%
AACCATATTATGCATGTGTT+TGG + chr4_4:64301841-64301860 Msa0694910:intron 30.0%
AACCAAACACATGCATAATA+TGG - chr4_4:64301846-64301865 None:intergenic 30.0%
ATCCAAGCTTCTTAAAATCA+CGG - chr4_4:64301911-64301930 None:intergenic 30.0%
! ATAGTGCCTATTTGTTAACA+CGG - chr4_4:64301973-64301992 None:intergenic 30.0%
!! CTATTTTGAAAAAGGCTAAC+AGG - chr4_4:64302162-64302181 None:intergenic 30.0%
!! CGTGACTTCTATTTTGAAAA+AGG - chr4_4:64302170-64302189 None:intergenic 30.0%
TTGTAAACTGTTGCAATCAT+AGG - chr4_4:64302416-64302435 None:intergenic 30.0%
AGAAAAGATTCGACAAAAAC+TGG - chr4_4:64302563-64302582 None:intergenic 30.0%
AATTTGCATTGTCATTGTAG+GGG + chr4_4:64302623-64302642 Msa0694910:intron 30.0%
!! TTATTTCTATCCATATAGGG+TGG + chr4_4:64302890-64302909 Msa0694910:intron 30.0%
TAACACAGTTTAATTATGGG+TGG - chr4_4:64303050-64303069 None:intergenic 30.0%
TAAACTGTGTTAGTGATACT+TGG + chr4_4:64303058-64303077 Msa0694910:intron 30.0%
ATTTGAATTGAGGTGATTTG+AGG + chr4_4:64303346-64303365 Msa0694910:intron 30.0%
CCTTATAAAATGCATCCAAA+CGG - chr4_4:64303579-64303598 None:intergenic 30.0%
! CGTTTGGATGCATTTTATAA+GGG + chr4_4:64303577-64303596 Msa0694910:CDS 30.0%
!! TACTTACTTGTTATGAGTAG+GGG - chr4_4:64303677-64303696 None:intergenic 30.0%
!! GTTACTTACTTGTTATGAGT+AGG - chr4_4:64303679-64303698 None:intergenic 30.0%
TGGTGTAAATGAGCATATAT+TGG - chr4_4:64303703-64303722 None:intergenic 30.0%
ACATCCTTCTATCTATACTA+AGG + chr4_4:64304011-64304030 Msa0694910:CDS 30.0%
!! GTTTTTGGACCACCAAAAAT+AGG + chr4_4:64300703-64300722 Msa0694910:five_prime_UTR 35.0%
! AAGCCATATGGTCCTATTTT+TGG - chr4_4:64300718-64300737 None:intergenic 35.0%
TATGGACATTGAAAGCCATA+TGG - chr4_4:64300730-64300749 None:intergenic 35.0%
GAGAGAGCAAAAACAATGTA+TGG - chr4_4:64300748-64300767 None:intergenic 35.0%
GCATGGATATAAGGAGATTT+AGG - chr4_4:64300778-64300797 None:intergenic 35.0%
!! GTAATTTGTTCTTTGGAAGC+TGG + chr4_4:64300828-64300847 Msa0694910:CDS 35.0%
TCAAATTGAGCTTCAACGAA+GGG - chr4_4:64300896-64300915 None:intergenic 35.0%
ATCAAATTGAGCTTCAACGA+AGG - chr4_4:64300897-64300916 None:intergenic 35.0%
!!! GTGGAGAGGATTTTTTGAAT+AGG + chr4_4:64301241-64301260 Msa0694910:CDS 35.0%
!!! GAGAGGATTTTTTGAATAGG+TGG + chr4_4:64301244-64301263 Msa0694910:CDS 35.0%
!!! GGATTTTTTGAATAGGTGGA+AGG + chr4_4:64301248-64301267 Msa0694910:CDS 35.0%
GAAAAGCTTGTAGTGACATT+AGG - chr4_4:64301354-64301373 None:intergenic 35.0%
! ATGTCACTACAAGCTTTTCT+AGG + chr4_4:64301355-64301374 Msa0694910:CDS 35.0%
TTAGACTCACACACATAGAT+AGG + chr4_4:64301433-64301452 Msa0694910:intron 35.0%
AAGCTTCTTAAAATCACGGT+GGG - chr4_4:64301907-64301926 None:intergenic 35.0%
! CTGTAGAGTTGGTCAAAATT+CGG + chr4_4:64301931-64301950 Msa0694910:intron 35.0%
ATACCACCGTGTTAACAAAT+AGG + chr4_4:64301964-64301983 Msa0694910:intron 35.0%
GATTGCAACAGTTTACAAGT+TGG + chr4_4:64302418-64302437 Msa0694910:intron 35.0%
TGTGTCATCAACATCAATCA+TGG + chr4_4:64302474-64302493 Msa0694910:intron 35.0%
GTGTCATCAACATCAATCAT+GGG + chr4_4:64302475-64302494 Msa0694910:intron 35.0%
TATCATATTGGGGATAGCAA+TGG - chr4_4:64302946-64302965 None:intergenic 35.0%
ACTATACCGTACACCATATT+TGG + chr4_4:64303118-64303137 Msa0694910:CDS 35.0%
TATCCACCAAATATGGTGTA+CGG - chr4_4:64303127-64303146 None:intergenic 35.0%
CTATAAAAGCTGGTAAAGCA+TGG + chr4_4:64303189-64303208 Msa0694910:CDS 35.0%
!! TTATTTGGCTTGCTTCATTC+AGG + chr4_4:64303509-64303528 Msa0694910:intron 35.0%
!! AGATGGTTCTAATCTAGTGA+GGG + chr4_4:64303546-64303565 Msa0694910:CDS 35.0%
! CCGTTTGGATGCATTTTATA+AGG + chr4_4:64303576-64303595 Msa0694910:CDS 35.0%
TTTATAAGGGATTGACCACT+TGG + chr4_4:64303590-64303609 Msa0694910:CDS 35.0%
TTATAAGGGATTGACCACTT+GGG + chr4_4:64303591-64303610 Msa0694910:CDS 35.0%
GTTAAAGAACATGAAGAAGC+AGG + chr4_4:64303942-64303961 Msa0694910:CDS 35.0%
GGATCCTTAGTATAGATAGA+AGG - chr4_4:64304018-64304037 None:intergenic 35.0%
ATCTATACTAAGGATCCAAC+TGG + chr4_4:64304021-64304040 Msa0694910:CDS 35.0%
ACATGATTAGAGCTGCATTT+AGG - chr4_4:64304101-64304120 None:intergenic 35.0%
!!! CCATATGGTCCTATTTTTGG+TGG - chr4_4:64300715-64300734 None:intergenic 40.0%
CCACCAAAAATAGGACCATA+TGG + chr4_4:64300712-64300731 Msa0694910:exon 40.0%
! TTGACTTTTGTGCTCTTGCT+GGG - chr4_4:64300802-64300821 None:intergenic 40.0%
!! TTTGACTTTTGTGCTCTTGC+TGG - chr4_4:64300803-64300822 None:intergenic 40.0%
CAGCTTGAAGAATCATAGAG+AGG - chr4_4:64300872-64300891 None:intergenic 40.0%
! TTTTTGTGGTGTAGGTTCAG+TGG + chr4_4:64301222-64301241 Msa0694910:intron 40.0%
GTCTTGTATGATTCATGCGT+CGG + chr4_4:64301326-64301345 Msa0694910:CDS 40.0%
CAATGTCTACTGTCTTCTTC+CGG + chr4_4:64301385-64301404 Msa0694910:CDS 40.0%
AATGTCTACTGTCTTCTTCC+GGG + chr4_4:64301386-64301405 Msa0694910:CDS 40.0%
CAAGCTTCTTAAAATCACGG+TGG - chr4_4:64301908-64301927 None:intergenic 40.0%
!! CACCGTGATTTTAAGAAGCT+TGG + chr4_4:64301906-64301925 Msa0694910:intron 40.0%
! GAGTTGGTCAAAATTCGGTT+TGG + chr4_4:64301936-64301955 Msa0694910:intron 40.0%
!! TTGGTCAAAATTCGGTTTGG+TGG + chr4_4:64301939-64301958 Msa0694910:intron 40.0%
!! TGGTCAAAATTCGGTTTGGT+GGG + chr4_4:64301940-64301959 Msa0694910:intron 40.0%
! TCTCGTTTGAGGCTGATAAT+AGG - chr4_4:64302384-64302403 None:intergenic 40.0%
TATTATCAGCCTCAAACGAG+AGG + chr4_4:64302383-64302402 Msa0694910:intron 40.0%
! TGCAACAGTTTACAAGTTGG+AGG + chr4_4:64302421-64302440 Msa0694910:intron 40.0%
CATCAATCATGGGTATGCTT+TGG + chr4_4:64302485-64302504 Msa0694910:intron 40.0%
TGGCCGGATATCCAAAAATA+TGG - chr4_4:64302926-64302945 None:intergenic 40.0%
TGGTGTACGGTATAGTTGTA+TGG - chr4_4:64303114-64303133 None:intergenic 40.0%
ATACCGTACACCATATTTGG+TGG + chr4_4:64303121-64303140 Msa0694910:CDS 40.0%
TGGTAAAGCATGGTTAGACA+TGG + chr4_4:64303199-64303218 Msa0694910:CDS 40.0%
ACATGTTGATCTTCAACTCC+TGG + chr4_4:64303222-64303241 Msa0694910:CDS 40.0%
! AGGTGGGATTTTATCAGAGA+TGG + chr4_4:64303529-64303548 Msa0694910:intron 40.0%
!! GAGATGGTTCTAATCTAGTG+AGG + chr4_4:64303545-64303564 Msa0694910:intron 40.0%
TTCTAATCTAGTGAGGGACA+TGG + chr4_4:64303552-64303571 Msa0694910:CDS 40.0%
TTGTTCACCACAACATTTGC+AGG - chr4_4:64303919-64303938 None:intergenic 40.0%
TGACTACAATCAATGCCAGT+TGG - chr4_4:64304039-64304058 None:intergenic 40.0%
!! CTGGCATTGATTGTAGTCAT+TGG + chr4_4:64304040-64304059 Msa0694910:CDS 40.0%
! AGGAGTATGTTCCAAGTATC+AGG - chr4_4:64304081-64304100 None:intergenic 40.0%
TTGCTGGGTGCATGGATATA+AGG - chr4_4:64300787-64300806 None:intergenic 45.0%
CTGGGTAAAGCACATGAATC+AGG - chr4_4:64300971-64300990 None:intergenic 45.0%
CTGATTCATGTGCTTTACCC+AGG + chr4_4:64300969-64300988 Msa0694910:CDS 45.0%
CGGAAGAAGACAGTAGACAT+TGG - chr4_4:64301387-64301406 None:intergenic 45.0%
GAAGCTTGGATCTGTAGAGT+TGG + chr4_4:64301920-64301939 Msa0694910:intron 45.0%
! GTGCCTATTTGTTAACACGG+TGG - chr4_4:64301970-64301989 None:intergenic 45.0%
ATATTGGGGATAGCAATGGC+CGG - chr4_4:64302942-64302961 None:intergenic 45.0%
AGATTGCATGCACAGGACTA+TGG + chr4_4:64303083-64303102 Msa0694910:CDS 45.0%
ACGCTGGACTCTATAAAAGC+TGG + chr4_4:64303179-64303198 Msa0694910:CDS 45.0%
TGATCTTCAACTCCTGGCAT+TGG + chr4_4:64303228-64303247 Msa0694910:CDS 45.0%
ATCCACACTGGAGATTTACG+AGG + chr4_4:64303254-64303273 Msa0694910:CDS 45.0%
TCCACACTGGAGATTTACGA+GGG + chr4_4:64303255-64303274 Msa0694910:CDS 45.0%
!! TTTGGCTTGCTTCATTCAGG+TGG + chr4_4:64303512-64303531 Msa0694910:intron 45.0%
!! TTGGCTTGCTTCATTCAGGT+GGG + chr4_4:64303513-64303532 Msa0694910:intron 45.0%
TCCTTTCTCCTTGAATCCAG+AGG + chr4_4:64303811-64303830 Msa0694910:intron 45.0%
CCTTTCTCCTTGAATCCAGA+GGG + chr4_4:64303812-64303831 Msa0694910:intron 45.0%
GAACCGAAGAAGACATGTAG+CGG + chr4_4:64303847-64303866 Msa0694910:CDS 45.0%
AACCGAAGAAGACATGTAGC+GGG + chr4_4:64303848-64303867 Msa0694910:CDS 45.0%
ATTACCGGGAACCACATATC+CGG + chr4_4:64303882-64303901 Msa0694910:CDS 45.0%
AACATTTGCAGGAGGTAGAG+GGG - chr4_4:64303908-64303927 None:intergenic 45.0%
CAACATTTGCAGGAGGTAGA+GGG - chr4_4:64303909-64303928 None:intergenic 45.0%
ACAACATTTGCAGGAGGTAG+AGG - chr4_4:64303910-64303929 None:intergenic 45.0%
TCTACCTCCTGCAAATGTTG+TGG + chr4_4:64303909-64303928 Msa0694910:CDS 45.0%
TTCACCACAACATTTGCAGG+AGG - chr4_4:64303916-64303935 None:intergenic 45.0%
AAGTATCAGGTAGTCCTGGT+AGG - chr4_4:64304068-64304087 None:intergenic 45.0%
TACCAGGACTACCTGATACT+TGG + chr4_4:64304067-64304086 Msa0694910:CDS 45.0%
TTCCAAGTATCAGGTAGTCC+TGG - chr4_4:64304072-64304091 None:intergenic 45.0%
!!! TTTTAACTATATATATATAT+AGG - chr4_4:64302010-64302029 None:intergenic 5.0%
!!! ATGTTTTAAATTTTTAAATA+AGG - chr4_4:64302721-64302740 None:intergenic 5.0%
!! AATTTAATTACTATATATAT+TGG + chr4_4:64304199-64304218 Msa0694910:three_prime_UTR 5.0%
ATCACGGTGGGATGTGCATT+GGG - chr4_4:64301895-64301914 None:intergenic 50.0%
AATCACGGTGGGATGTGCAT+TGG - chr4_4:64301896-64301915 None:intergenic 50.0%
GGTTGACATCCTCTCGTTTG+AGG - chr4_4:64302395-64302414 None:intergenic 50.0%
CTTGGAGAGATTGCATGCAC+AGG + chr4_4:64303076-64303095 Msa0694910:intron 50.0%
! TGTTGGTAGAGTGCTTACGC+TGG + chr4_4:64303163-64303182 Msa0694910:CDS 50.0%
! TCTTCAACTCCTGGCATTGG+TGG + chr4_4:64303231-64303250 Msa0694910:CDS 50.0%
ACCCTCGTAAATCTCCAGTG+TGG - chr4_4:64303259-64303278 None:intergenic 50.0%
! ATTGACCACTTGGGCTGGAT+GGG + chr4_4:64303600-64303619 Msa0694910:CDS 50.0%
CCCTCTGGATTCAAGGAGAA+AGG - chr4_4:64303815-64303834 None:intergenic 50.0%
AATCCTGCCCTCTGGATTCA+AGG - chr4_4:64303822-64303841 None:intergenic 50.0%
TTGAATCCAGAGGGCAGGAT+TGG + chr4_4:64303821-64303840 Msa0694910:intron 50.0%
TTCATTCCAATCCTGCCCTC+TGG - chr4_4:64303830-64303849 None:intergenic 50.0%
ACCGAAGAAGACATGTAGCG+GGG + chr4_4:64303849-64303868 Msa0694910:CDS 50.0%
TCCCCGCTACATGTCTTCTT+CGG - chr4_4:64303853-64303872 None:intergenic 50.0%
CGGGGAAGTTGAACCATTAC+CGG + chr4_4:64303867-64303886 Msa0694910:CDS 50.0%
GGGGAAGTTGAACCATTACC+GGG + chr4_4:64303868-64303887 Msa0694910:CDS 50.0%
GGATATGTGGTTCCCGGTAA+TGG - chr4_4:64303883-64303902 None:intergenic 50.0%
! TGTAGTCATTGGTGCCTACC+AGG + chr4_4:64304051-64304070 Msa0694910:CDS 50.0%
TTGTGCTCTTGCTGGGTGCA+TGG - chr4_4:64300795-64300814 None:intergenic 55.0%
GTGGTGTAGGTTCAGTGGAG+AGG + chr4_4:64301227-64301246 Msa0694910:intron 55.0%
AGTGTGGATCCACCAATGCC+AGG - chr4_4:64303243-64303262 None:intergenic 55.0%
!! TGGCATTGGTGGATCCACAC+TGG + chr4_4:64303242-64303261 Msa0694910:CDS 55.0%
AGTGAGGGACATGGACCGTT+TGG + chr4_4:64303561-64303580 Msa0694910:CDS 55.0%
AAGGGATTGACCACTTGGGC+TGG + chr4_4:64303595-64303614 Msa0694910:CDS 55.0%
! GATTGACCACTTGGGCTGGA+TGG + chr4_4:64303599-64303618 Msa0694910:CDS 55.0%
ATCAACCCATCCAGCCCAAG+TGG - chr4_4:64303608-64303627 None:intergenic 55.0%
TCTCCTTGAATCCAGAGGGC+AGG + chr4_4:64303816-64303835 Msa0694910:intron 55.0%
AGGTAGAGGGGCCGGATATG+TGG - chr4_4:64303896-64303915 None:intergenic 60.0%
TTTGCAGGAGGTAGAGGGGC+CGG - chr4_4:64303904-64303923 None:intergenic 60.0%
GGGGCCGGATATGTGGTTCC+CGG - chr4_4:64303889-64303908 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 64300703 64304274 64300703 ID=Msa0694910;Name=Msa0694910
chr4_4 mRNA 64300703 64304274 64300703 ID=Msa0694910-mRNA-1;Parent=Msa0694910;Name=Msa0694910-mRNA-1;_AED=0.15;_eAED=0.15;_QI=28|0.6|0.66|1|0.8|0.66|6|127|353
chr4_4 exon 64300703 64300990 64300703 ID=Msa0694910-mRNA-1:exon:18402;Parent=Msa0694910-mRNA-1
chr4_4 exon 64301236 64301407 64301236 ID=Msa0694910-mRNA-1:exon:18403;Parent=Msa0694910-mRNA-1
chr4_4 exon 64303083 64303276 64303083 ID=Msa0694910-mRNA-1:exon:18404;Parent=Msa0694910-mRNA-1
chr4_4 exon 64303546 64303689 64303546 ID=Msa0694910-mRNA-1:exon:18405;Parent=Msa0694910-mRNA-1
chr4_4 exon 64303831 64304138 64303831 ID=Msa0694910-mRNA-1:exon:18406;Parent=Msa0694910-mRNA-1
chr4_4 exon 64304164 64304274 64304164 ID=Msa0694910-mRNA-1:exon:18407;Parent=Msa0694910-mRNA-1
chr4_4 five_prime_UTR 64300703 64300730 64300703 ID=Msa0694910-mRNA-1:five_prime_utr;Parent=Msa0694910-mRNA-1
chr4_4 CDS 64300731 64300990 64300731 ID=Msa0694910-mRNA-1:cds;Parent=Msa0694910-mRNA-1
chr4_4 CDS 64301236 64301407 64301236 ID=Msa0694910-mRNA-1:cds;Parent=Msa0694910-mRNA-1
chr4_4 CDS 64303083 64303276 64303083 ID=Msa0694910-mRNA-1:cds;Parent=Msa0694910-mRNA-1
chr4_4 CDS 64303546 64303689 64303546 ID=Msa0694910-mRNA-1:cds;Parent=Msa0694910-mRNA-1
chr4_4 CDS 64303831 64304122 64303831 ID=Msa0694910-mRNA-1:cds;Parent=Msa0694910-mRNA-1
chr4_4 three_prime_UTR 64304123 64304138 64304123 ID=Msa0694910-mRNA-1:three_prime_utr;Parent=Msa0694910-mRNA-1
chr4_4 three_prime_UTR 64304164 64304274 64304164 ID=Msa0694910-mRNA-1:three_prime_utr;Parent=Msa0694910-mRNA-1
Gene Sequence

>Msa0694910

ATGGCTTTCAATGTCCATACATTGTTTTTGCTCTCTCTGTCTAGCCTAAATCTCCTTATATCCATGCACCCAGCAAGAGCACAAAAGTCAAATAATTGTAATTTGTTCTTTGGAAGCTGGGTGATTGACTCTTCATATCCTCTCTATGATTCTTCAAGCTGTCCCTTCGTTGAAGCTCAATTTGATTGCCAAAAATATGGAAGACCTGATAATCAGTATCTTAAATACTCTTGGAAGCCTGATTCATGTGCTTTACCCAGGTTCAGTGGAGAGGATTTTTTGAATAGGTGGAAGGATAAGAAGATAATGTTTGTAGGTGATTCATTGAGTCTAAACATGTGGGAATCATTGTCTTGTATGATTCATGCGTCGGTTCCTAATGTCACTACAAGCTTTTCTAGGAAAGAACCAATGTCTACTGTCTTCTTCCGGAGATTGCATGCACAGGACTATGGAGTAACCATACAACTATACCGTACACCATATTTGGTGGATATAATAATTCAAGAAGATGTTGGTAGAGTGCTTACGCTGGACTCTATAAAAGCTGGTAAAGCATGGTTAGACATGGACATGTTGATCTTCAACTCCTGGCATTGGTGGATCCACACTGGAGATTTACGAGGAGATGGTTCTAATCTAGTGAGGGACATGGACCGTTTGGATGCATTTTATAAGGGATTGACCACTTGGGCTGGATGGGTTGATGCAAATGTTGACCCTACTAAAACTAAACTCTTCTTTCAAGGAATTTCCCCTACTCATAACAAAGGGCAGGATTGGAATGAACCGAAGAAGACATGTAGCGGGGAAGTTGAACCATTACCGGGAACCACATATCCGGCCCCTCTACCTCCTGCAAATGTTGTGGTGAACAAAGTGTTAAAGAACATGAAGAAGCAGGTTTATCTACTTGACATAACACTTCTCTCACAACTAAGAAAAGATGCACATCCTTCTATCTATACTAAGGATCCAACTGGCATTGATTGTAGTCATTGGTGCCTACCAGGACTACCTGATACTTGGAACATACTCCTAAATGCAGCTCTAATCATGTGA
Protein sequence

>Msa0694910

MAFNVHTLFLLSLSSLNLLISMHPARAQKSNNCNLFFGSWVIDSSYPLYDSSSCPFVEAQFDCQKYGRPDNQYLKYSWKPDSCALPRFSGEDFLNRWKDKKIMFVGDSLSLNMWESLSCMIHASVPNVTTSFSRKEPMSTVFFRRLHAQDYGVTIQLYRTPYLVDIIIQEDVGRVLTLDSIKAGKAWLDMDMLIFNSWHWWIHTGDLRGDGSNLVRDMDRLDAFYKGLTTWAGWVDANVDPTKTKLFFQGISPTHNKGQDWNEPKKTCSGEVEPLPGTTYPAPLPPANVVVNKVLKNMKKQVYLLDITLLSQLRKDAHPSIYTKDPTGIDCSHWCLPGLPDTWNILLNAALIM*