AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0608280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608280 MtrunA17_Chr4g0040481 90.816 392 30 4 1 389 1 389 0.0 642
Msa0608280 MtrunA17_Chr2g0324891 59.233 417 115 19 1 389 1 390 2.43e-135 394
Msa0608280 MtrunA17_Chr5g0400241 35.111 225 112 7 121 331 267 471 3.77e-28 116
Msa0608280 MtrunA17_Chr4g0054981 41.007 139 69 4 145 275 299 432 2.77e-26 110
Msa0608280 MtrunA17_Chr4g0009851 38.710 93 56 1 120 212 134 225 6.94e-18 84.0
Msa0608280 MtrunA17_Chr1g0161431 29.798 198 118 5 81 277 137 314 1.41e-17 84.7
Msa0608280 MtrunA17_Chr3g0137101 47.368 76 40 0 148 223 190 265 2.09e-17 84.3
Msa0608280 MtrunA17_Chr1g0149841 54.545 66 30 0 153 218 188 253 2.53e-16 80.9
Msa0608280 MtrunA17_Chr2g0304841 54.839 62 28 0 151 212 208 269 4.67e-16 80.1
Msa0608280 MtrunA17_Chr4g0067981 56.667 60 26 0 155 214 214 273 7.14e-16 79.7
Msa0608280 MtrunA17_Chr4g0009981 38.202 89 54 1 124 212 85 172 1.04e-15 76.6
Msa0608280 MtrunA17_Chr2g0294911 40.230 87 47 1 155 236 210 296 7.36e-15 76.3
Msa0608280 MtrunA17_Chr4g0009901 38.710 93 56 1 120 212 133 224 8.11e-15 74.3
Msa0608280 MtrunA17_Chr7g0255711 51.667 60 29 0 155 214 90 149 9.52e-15 74.3
Msa0608280 MtrunA17_Chr8g0376881 47.826 69 36 0 155 223 208 276 2.67e-14 74.7
Msa0608280 MtrunA17_Chr4g0031961 39.506 81 49 0 143 223 118 198 5.54e-14 72.4
Msa0608280 MtrunA17_Chr4g0062921 36.000 125 60 4 108 212 168 292 2.04e-13 71.6
Msa0608280 MtrunA17_Chr3g0123131 44.928 69 38 0 155 223 198 266 2.95e-13 71.2
Msa0608280 MtrunA17_Chr7g0275401 35.246 122 72 2 98 212 127 248 6.17e-13 68.9
Msa0608280 MtrunA17_Chr7g0275891 32.117 137 57 4 151 255 186 318 9.06e-13 68.9
Msa0608280 MtrunA17_Chr6g0461641 56.364 55 23 1 158 211 44 98 1.32e-12 68.9
Msa0608280 MtrunA17_Chr4g0074401 49.091 55 28 0 154 208 201 255 2.26e-12 68.6
Msa0608280 MtrunA17_Chr4g0045361 41.573 89 47 2 152 239 270 354 3.49e-12 67.8
Msa0608280 MtrunA17_Chr6g0473731 44.118 68 38 0 155 222 94 161 4.68e-12 67.4
Msa0608280 MtrunA17_Chr1g0198601 36.275 102 52 2 147 235 202 303 5.86e-12 66.6
Msa0608280 MtrunA17_Chr2g0321461 55.769 52 22 1 158 208 47 98 6.30e-12 66.6
Msa0608280 MtrunA17_Chr1g0149831 46.970 66 32 1 153 218 125 187 7.72e-12 64.7
Msa0608280 MtrunA17_Chr3g0134481 48.936 47 24 0 161 207 137 183 1.38e-11 63.2
Msa0608280 MtrunA17_Chr4g0074411 49.091 55 28 0 154 208 199 253 1.41e-11 66.2
Msa0608280 MtrunA17_Chr4g0009991 39.744 78 46 1 128 205 40 116 1.55e-11 61.2
Msa0608280 MtrunA17_Chr1g0175081 50.000 52 25 1 158 208 24 75 2.23e-11 60.5
Msa0608280 MtrunA17_Chr5g0417521 44.000 75 36 3 158 231 17 86 3.27e-11 64.3
Msa0608280 MtrunA17_Chr1g0149851 42.667 75 28 1 153 212 188 262 3.50e-11 65.1
Msa0608280 MtrunA17_Chr7g0254981 47.368 76 35 3 137 208 29 103 3.90e-11 63.5
Msa0608280 MtrunA17_Chr5g0422621 42.029 69 39 1 145 212 187 255 4.26e-11 63.9
Msa0608280 MtrunA17_Chr1g0190301 46.377 69 34 2 144 211 165 231 9.41e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608280 AT2G20570.1 44.258 418 170 19 1 389 1 384 4.67e-77 245
Msa0608280 AT2G20570.2 42.627 434 170 20 1 389 1 400 5.73e-73 235
Msa0608280 AT5G44190.1 40.097 414 158 19 1 387 1 351 1.21e-63 209
Msa0608280 AT5G44190.2 45.763 295 112 13 1 277 1 265 3.03e-61 202
Msa0608280 AT2G20570.3 34.450 418 164 20 1 389 1 337 1.97e-34 131
Msa0608280 AT4G18020.8 35.470 234 129 5 37 264 191 408 2.17e-29 119
Msa0608280 AT4G18020.11 35.470 234 129 5 37 264 191 408 2.17e-29 119
Msa0608280 AT4G18020.4 35.470 234 129 5 37 264 191 408 2.17e-29 119
Msa0608280 AT4G18020.5 35.470 234 129 5 37 264 191 408 2.17e-29 119
Msa0608280 AT4G18020.10 35.470 234 129 5 37 264 191 408 2.17e-29 119
Msa0608280 AT4G18020.7 35.470 234 129 5 37 264 191 408 2.17e-29 119
Msa0608280 AT4G18020.3 35.470 234 129 5 37 264 191 408 4.10e-29 119
Msa0608280 AT4G18020.2 35.470 234 129 5 37 264 191 408 4.10e-29 119
Msa0608280 AT4G18020.1 35.470 234 129 5 37 264 191 408 4.10e-29 119
Msa0608280 AT4G18020.6 35.470 234 129 5 37 264 191 408 4.10e-29 119
Msa0608280 AT4G18020.9 35.470 234 129 5 37 264 191 408 4.10e-29 119
Msa0608280 AT5G58080.1 55.738 61 27 0 154 214 192 252 3.00e-16 81.3
Msa0608280 AT3G16857.3 39.815 108 56 2 107 214 127 225 1.21e-15 79.3
Msa0608280 AT3G16857.2 39.815 108 56 2 107 214 197 295 1.64e-15 79.0
Msa0608280 AT2G01760.3 53.125 64 30 0 151 214 130 193 1.69e-15 77.4
Msa0608280 AT3G16857.1 39.815 108 56 2 107 214 197 295 1.81e-15 79.0
Msa0608280 AT5G59570.2 40.206 97 46 2 135 231 132 216 2.95e-15 76.3
Msa0608280 AT5G59570.1 40.206 97 46 2 135 231 132 216 2.95e-15 76.3
Msa0608280 AT2G01760.1 53.125 64 30 0 151 214 195 258 3.22e-15 77.0
Msa0608280 AT3G10760.1 41.772 79 46 0 136 214 85 163 3.37e-15 76.6
Msa0608280 AT2G01760.2 53.125 64 30 0 151 214 223 286 3.75e-15 77.0
Msa0608280 AT5G05090.1 51.667 60 29 0 155 214 80 139 5.10e-15 75.1
Msa0608280 AT2G25180.1 50.820 61 30 0 154 214 193 253 1.86e-14 75.5
Msa0608280 AT4G31920.1 52.542 59 28 0 150 208 177 235 2.17e-14 75.5
Msa0608280 AT3G46640.2 46.479 71 36 1 155 223 143 213 2.39e-14 73.9
Msa0608280 AT3G46640.1 46.479 71 36 1 155 223 143 213 2.39e-14 73.9
Msa0608280 AT2G03500.1 36.154 130 73 2 148 267 225 354 3.26e-14 74.3
Msa0608280 AT3G46640.3 46.479 71 36 1 155 223 143 213 4.24e-14 73.2
Msa0608280 AT5G07210.1 36.585 123 63 3 109 224 180 294 5.71e-14 74.3
Msa0608280 AT5G07210.2 36.585 123 63 3 109 224 172 286 6.59e-14 73.9
Msa0608280 AT2G40970.1 48.333 60 31 0 155 214 104 163 8.81e-14 71.2
Msa0608280 AT3G04030.1 57.407 54 22 1 156 208 45 98 1.54e-12 68.9
Msa0608280 AT3G04030.3 57.407 54 22 1 156 208 45 98 1.80e-12 68.9
Msa0608280 AT5G18240.2 59.259 54 21 1 156 208 45 98 2.38e-12 68.6
Msa0608280 AT5G18240.3 59.259 54 21 1 156 208 45 98 2.38e-12 68.6
Msa0608280 AT5G18240.5 59.259 54 21 1 156 208 45 98 2.69e-12 68.2
Msa0608280 AT5G18240.1 59.259 54 21 1 156 208 45 98 2.84e-12 68.2
Msa0608280 AT5G18240.4 59.259 54 21 1 156 208 45 98 2.84e-12 68.2
Msa0608280 AT2G27070.1 37.383 107 54 2 121 215 179 284 4.44e-12 68.2
Msa0608280 AT1G13300.1 40.000 90 50 2 124 212 152 238 4.51e-12 67.4
Msa0608280 AT1G67710.2 33.884 121 70 2 104 214 67 187 2.18e-11 65.9
Msa0608280 AT1G67710.3 33.884 121 70 2 104 214 58 178 2.80e-11 65.5
Msa0608280 AT3G04030.2 52.830 53 25 0 156 208 45 97 2.95e-11 65.1
Msa0608280 AT1G67710.1 33.884 121 70 2 104 214 132 252 3.92e-11 65.1
Msa0608280 AT1G25550.1 41.892 74 42 1 141 213 194 267 8.04e-11 63.5
Msa0608280 AT5G45580.1 53.846 52 23 1 158 208 9 60 8.58e-11 62.8
Msa0608280 AT1G68670.1 36.111 108 64 2 134 236 193 300 9.77e-11 63.2

Find 164 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCTTGGTTTCCATCTT+TGG 0.144453 4_2:-55293332 None:intergenic
GGCGGTGCCTTCAAGGATTT+TGG 0.156203 4_2:+55294513 Msa0608280:CDS
GGCCGGATCATTGCGTTTAT+TGG 0.197058 4_2:-55296531 None:intergenic
CACCTCAAGATCCTTCATTT+TGG 0.206831 4_2:+55295091 Msa0608280:CDS
ATTCTTCTCTTCCGCTTTGC+TGG 0.231572 4_2:-55293581 None:intergenic
CCTTTCGGAAGGAAATTTAT+TGG 0.245412 4_2:+55293408 Msa0608280:CDS
TGGAGACATCTTGCCGGATT+TGG 0.249570 4_2:+55293474 Msa0608280:CDS
TTCTTCTCTTCCGCTTTGCT+GGG 0.303962 4_2:-55293580 None:intergenic
ATGCCATGGGGTGGGATGCC+GGG 0.306670 4_2:-55296864 None:intergenic
CCCATTTGGTTATTATTAGA+AGG 0.309501 4_2:+55293243 Msa0608280:five_prime_UTR
ATGGGGACATCCTTCAAATA+TGG 0.339407 4_2:+55294999 Msa0608280:CDS
AAACCCACGGTGGGTGCAGC+TGG 0.343991 4_2:-55294916 None:intergenic
GCCACGTGGCACACCCTGAT+TGG 0.346219 4_2:-55297368 None:intergenic
TTATATAACATCACCCAATC+AGG 0.358673 4_2:+55297354 Msa0608280:three_prime_UTR
CATGCCATGGGGTGGGATGC+CGG 0.361244 4_2:-55296865 None:intergenic
AACTTCGACGACTTCTTTGT+TGG 0.363264 4_2:+55293439 Msa0608280:CDS
ATACATGTAAGGGTCTAAAA+TGG 0.371409 4_2:-55294978 None:intergenic
TGGGGTGGGATGCCGGGGAT+TGG 0.376158 4_2:-55296858 None:intergenic
TGAGGAGGAGGAGGATGAGG+GGG 0.377528 4_2:-55294940 None:intergenic
TGAGGATAAAACAAGTTGTT+CGG 0.385599 4_2:+55293618 Msa0608280:CDS
TCATCCTCCTCCTCCTCATA+TGG 0.386130 4_2:+55294945 Msa0608280:CDS
GAGAACGTGTGTTTAAATAA+TGG 0.392040 4_2:-55293078 None:intergenic
TGTTTAAATAATGGAGAGTT+TGG 0.392089 4_2:-55293069 None:intergenic
ACAAATCTCCTGTGCAAGTC+TGG 0.392453 4_2:-55294460 None:intergenic
GATGAATTCCTCGGCGTCAT+TGG 0.395120 4_2:+55293552 Msa0608280:CDS
GAGGATAAAACAAGTTGTTC+GGG 0.407784 4_2:+55293619 Msa0608280:CDS
GAGGAGGAGGAGGATGAGGG+GGG 0.414573 4_2:-55294939 None:intergenic
ACCTTCTAATAATAACCAAA+TGG 0.414789 4_2:-55293244 None:intergenic
TTAAAAGATGCAAGTGGAAA+AGG 0.416680 4_2:+55293700 Msa0608280:CDS
CGGCGACTTCGGTGACCTTT+CGG 0.423356 4_2:+55293393 Msa0608280:CDS
GAGAGCATAGACGCAGCTAT+TGG 0.425120 4_2:+55297244 Msa0608280:CDS
TGGCAGGTTGACGGCGGCGG+CGG 0.427632 4_2:-55297581 None:intergenic
TAACATTGCTAGTCACCTTC+AGG 0.436461 4_2:+55294567 Msa0608280:CDS
ATAATCTCCAAAATCCTTGA+AGG 0.440155 4_2:-55294520 None:intergenic
TATATATTGAGTTTCCCATT+TGG 0.442561 4_2:+55293229 Msa0608280:five_prime_UTR
ATATGAGGAGGAGGAGGATG+AGG 0.442642 4_2:-55294943 None:intergenic
GAGAACTCAGCAAACATCTC+TGG 0.443524 4_2:-55293505 None:intergenic
ACCACTGTTGTCAGAGGTGG+TGG 0.450953 4_2:-55296570 None:intergenic
TGGACAAGGCGGTGCCTTCA+AGG 0.452098 4_2:+55294506 Msa0608280:CDS
ACAATTACAACCGGCGACTT+CGG 0.457007 4_2:+55293382 Msa0608280:CDS
TCATCATGCACTTGAAGAAA+AGG 0.457613 4_2:+55297299 Msa0608280:CDS
ACCTTATGATGACACATTCA+TGG 0.457686 4_2:-55297601 None:intergenic
GGTGGGAAACAATGTGTTCC+TGG 0.458402 4_2:-55296552 None:intergenic
TCGAAGAAGTGACCGGGAGC+AGG 0.463586 4_2:-55296918 None:intergenic
CATGAGAGTGGCTGCGGTGT+AGG 0.464099 4_2:-55295045 None:intergenic
AATCAAAGGAGACAAGTATA+TGG 0.464153 4_2:+55294856 Msa0608280:CDS
GAAGGTGACTAGCAATGTTA+TGG 0.464906 4_2:-55294564 None:intergenic
GAATTCAAAGAAAGATTATG+AGG 0.466750 4_2:+55293600 Msa0608280:CDS
CTCCAATAAACGCAATGATC+CGG 0.470333 4_2:+55296529 Msa0608280:CDS
TGCCATGGGGTGGGATGCCG+GGG 0.471359 4_2:-55296863 None:intergenic
AAGCAGCAAGTTGGAATCAA+AGG 0.471928 4_2:+55294842 Msa0608280:CDS
GCTGAGTTCTCCGTTAGTTA+CGG 0.472120 4_2:+55293517 Msa0608280:CDS
TCCTTCTCTACATACTCTCT+TGG 0.472407 4_2:+55292990 Msa0608280:five_prime_UTR
ATGAGGAGGAGGAGGATGAG+GGG 0.473493 4_2:-55294941 None:intergenic
TGTTTATTGTGCAGGTGGAT+TGG 0.477271 4_2:+55294434 Msa0608280:intron
CCACGTGGCACACCCTGATT+GGG 0.478169 4_2:-55297367 None:intergenic
AGACAAGTATATGGTGGTGT+AGG 0.478471 4_2:+55294865 Msa0608280:CDS
GACAAGTATATGGTGGTGTA+GGG 0.478876 4_2:+55294866 Msa0608280:CDS
TCTGGATCCATCTCCAAATC+CGG 0.478975 4_2:-55293487 None:intergenic
CTGAGGAACACTACCAAAGA+TGG 0.481384 4_2:+55293319 Msa0608280:CDS
CCTTCTAATAATAACCAAAT+GGG 0.482415 4_2:-55293243 None:intergenic
ACGGTGGGTGCAGCTGGCCA+TGG 0.483242 4_2:-55294910 None:intergenic
CGTCGATGGAGACATCTTGC+CGG 0.485716 4_2:+55293468 Msa0608280:CDS
GCCATGAATGTGTCATCATA+AGG 0.488918 4_2:+55297600 Msa0608280:three_prime_UTR
AACATGCAGAGACCATCATT+TGG 0.489976 4_2:+55296831 Msa0608280:intron
ACGGCGGCGGCGGCGACCGA+TGG 0.492397 4_2:-55297571 None:intergenic
GCGGCGGCGGCGACCGATGG+TGG 0.496188 4_2:-55297568 None:intergenic
GATCTGCTTTACACATGCCA+TGG 0.496492 4_2:-55296878 None:intergenic
ATTAGCACCCTTCCTGCTCC+CGG 0.500715 4_2:+55296906 Msa0608280:CDS
AGGATGTCCCCATACATGTA+AGG 0.503004 4_2:-55294989 None:intergenic
CATCTTGCCGGATTTGGAGA+TGG 0.503664 4_2:+55293480 Msa0608280:CDS
AGAAAAGCTTAGAAATGGTA+GGG 0.504508 4_2:-55293134 None:intergenic
GAGAAAAGCTTAGAAATGGT+AGG 0.505440 4_2:-55293135 None:intergenic
GAAGTGACCGGGAGCAGGAA+GGG 0.509953 4_2:-55296913 None:intergenic
GGGAGTACAACAATTACAAC+CGG 0.510212 4_2:+55293373 Msa0608280:CDS
TCATGGCAGGTTGACGGCGG+CGG 0.515383 4_2:-55297584 None:intergenic
ATCTGCTTTACACATGCCAT+GGG 0.515933 4_2:-55296877 None:intergenic
TTTCTTCAAGTGCATGATGA+GGG 0.516517 4_2:-55297296 None:intergenic
TGATTCCTCGCCGTAACTAA+CGG 0.519226 4_2:-55293527 None:intergenic
TTTGTTGGCATCAACGTCGA+TGG 0.522723 4_2:+55293454 Msa0608280:CDS
GAAGGATCTTGAGGTGGTGG+AGG 0.522935 4_2:-55295084 None:intergenic
AGATGGAAACCAAGAAGAGA+TGG 0.523180 4_2:+55293336 Msa0608280:CDS
AATGAAGGATCTTGAGGTGG+TGG 0.528793 4_2:-55295087 None:intergenic
GCCAAGAGAGTATGTAGAGA+AGG 0.529810 4_2:-55292991 None:intergenic
TGGCCAGCTGCACCCACCGT+GGG 0.530701 4_2:+55294913 Msa0608280:CDS
CCAATAAATTTCCTTCCGAA+AGG 0.532064 4_2:-55293408 None:intergenic
AAAGACTGGTCCATATTTGA+AGG 0.536097 4_2:-55295009 None:intergenic
GGAAACAATGTGTTCCTGGC+CGG 0.538523 4_2:-55296548 None:intergenic
TAAGAGAACACGTGAGTGAG+AGG 0.540888 4_2:+55297431 Msa0608280:three_prime_UTR
AAAACAAGTTGTTCGGGTCA+AGG 0.542391 4_2:+55293625 Msa0608280:CDS
TGGGAAGTCGAAGAAGTGAC+CGG 0.545060 4_2:-55296925 None:intergenic
ACACATTCATGGCAGGTTGA+CGG 0.545553 4_2:-55297590 None:intergenic
TGGTGCAATGGTGCCATATG+AGG 0.549696 4_2:-55294958 None:intergenic
GGGATTGGACATCCAAATGA+TGG 0.551089 4_2:-55296843 None:intergenic
CGAAAGGTCACCGAAGTCGC+CGG 0.551097 4_2:-55293392 None:intergenic
AGTAAATCTAAGAATCCTCA+AGG 0.553031 4_2:+55293736 Msa0608280:CDS
CTACACCGCAGCCACTCTCA+TGG 0.554231 4_2:+55295046 Msa0608280:CDS
GCTTTACACATGCCATGGGG+TGG 0.557961 4_2:-55296873 None:intergenic
TATGAGGAGGAGGAGGATGA+GGG 0.561254 4_2:-55294942 None:intergenic
AACTCACTATGTCACACAAG+TGG 0.561314 4_2:-55297325 None:intergenic
TTGGCATTCTCACCACCAGC+AGG 0.563830 4_2:+55295110 Msa0608280:CDS
GGAAGGAGGAAGCAGAATGT+TGG 0.566225 4_2:-55297494 None:intergenic
AAGTTGTTCGGGTCAAGGCT+CGG 0.571650 4_2:+55293630 Msa0608280:CDS
GAAACTCAATATATAAAACT+AGG 0.576041 4_2:-55293221 None:intergenic
TGCCAAAATGAAGGATCTTG+AGG 0.576157 4_2:-55295093 None:intergenic
TCATTTGGATGTCCAATCCC+CGG 0.576190 4_2:+55296846 Msa0608280:CDS
TCTTCTCTTCCGCTTTGCTG+GGG 0.576625 4_2:-55293579 None:intergenic
CTTTACACATGCCATGGGGT+GGG 0.577896 4_2:-55296872 None:intergenic
TATGATGACACATTCATGGC+AGG 0.579474 4_2:-55297597 None:intergenic
CTTCAAGTGCATGATGAGGG+TGG 0.579764 4_2:-55297293 None:intergenic
ATGGCCAGCTGCACCCACCG+TGG 0.581860 4_2:+55294912 Msa0608280:CDS
ATCCCCGGCATCCCACCCCA+TGG 0.583757 4_2:+55296861 Msa0608280:CDS
GGTGCCATATGAGGAGGAGG+AGG 0.584245 4_2:-55294949 None:intergenic
CGGCGGCGACCGATGGTGGA+CGG 0.584961 4_2:-55297564 None:intergenic
GTGCAAGCTGTGGAACAGCT+AGG 0.587705 4_2:+55294481 Msa0608280:CDS
ATAAACGCAATGATCCGGCC+AGG 0.588149 4_2:+55296534 Msa0608280:CDS
GTGAAGCAGAAGCAGCAAGT+TGG 0.590428 4_2:+55294833 Msa0608280:CDS
GGATGTCCCCATACATGTAA+GGG 0.590827 4_2:-55294988 None:intergenic
AATGGTGCCATATGAGGAGG+AGG 0.593332 4_2:-55294952 None:intergenic
GGTGTCCATGAGAGTGGCTG+CGG 0.595402 4_2:-55295051 None:intergenic
CATGATGAGGGTGGCAACCA+TGG 0.596831 4_2:-55297284 None:intergenic
GACTTCGGTGACCTTTCGGA+AGG 0.597816 4_2:+55293397 Msa0608280:CDS
ATTGGACACCAGACTTGCAC+AGG 0.600253 4_2:+55294452 Msa0608280:CDS
GAAGGTTGAGTACTTGAGTG+TGG 0.600374 4_2:+55293261 Msa0608280:five_prime_UTR
TTTAGACCCTTACATGTATG+GGG 0.601372 4_2:+55294982 Msa0608280:CDS
TCACTTCTTCGACTTCCCAC+CGG 0.602135 4_2:+55296929 Msa0608280:CDS
GCCACTCTCATGGACACCAT+CGG 0.612134 4_2:+55295056 Msa0608280:CDS
AGAAGTGACCGGGAGCAGGA+AGG 0.612201 4_2:-55296914 None:intergenic
GGAATCAGAGATGAATTCCT+CGG 0.620947 4_2:+55293543 Msa0608280:CDS
GCCACATGTGCAGAAAAGAC+TGG 0.621691 4_2:-55295023 None:intergenic
AGCTGTGGAACAGCTAGGAG+TGG 0.623334 4_2:+55294486 Msa0608280:CDS
AGAGAGAAGTGATCAGTCCA+TGG 0.624850 4_2:+55294893 Msa0608280:CDS
TATATAACATCACCCAATCA+GGG 0.629060 4_2:+55297355 Msa0608280:three_prime_UTR
AAGAGAACACGTGAGTGAGA+GGG 0.631537 4_2:+55297432 Msa0608280:three_prime_UTR
TCAAGGGAAGAGAAAAGTCA+AGG 0.631578 4_2:+55293753 Msa0608280:CDS
GGCATGTGTAAAGCAGATCA+CGG 0.631742 4_2:+55296882 Msa0608280:CDS
GGGAAGTCGAAGAAGTGACC+GGG 0.633060 4_2:-55296924 None:intergenic
CAGGAGATTTGTGCAAGCTG+TGG 0.634615 4_2:+55294471 Msa0608280:CDS
ACAAGTATATGGTGGTGTAG+GGG 0.636855 4_2:+55294867 Msa0608280:CDS
TGGTGAGAATGCCAAAATGA+AGG 0.637260 4_2:-55295102 None:intergenic
ACCGATGGTGTCCATGAGAG+TGG 0.639166 4_2:-55295057 None:intergenic
GTTACCACTGTTGTCAGAGG+TGG 0.641294 4_2:-55296573 None:intergenic
CAAAGGAGACAAGTATATGG+TGG 0.641945 4_2:+55294859 Msa0608280:CDS
CCCAATCAGGGTGTGCCACG+TGG 0.650811 4_2:+55297367 Msa0608280:three_prime_UTR
TCTTTGGTAGTGTTCCTCAG+TGG 0.652633 4_2:-55293316 None:intergenic
GTAAATCTAAGAATCCTCAA+GGG 0.653344 4_2:+55293737 Msa0608280:CDS
ATAGTTACCACTGTTGTCAG+AGG 0.658785 4_2:-55296576 None:intergenic
GGAACAGCTAGGAGTGGACA+AGG 0.659903 4_2:+55294492 Msa0608280:CDS
TAGAGAGAGCTACCTGCTGG+TGG 0.667063 4_2:-55295122 None:intergenic
GGGGTTCTCCAATGACGCCG+AGG 0.684455 4_2:-55293560 None:intergenic
GGTGGTGGAGGAGCTACCGA+TGG 0.688749 4_2:-55295072 None:intergenic
GAGTAAAAGAGATGAATCTG+TGG 0.689938 4_2:+55293663 Msa0608280:CDS
AAGGGTCTAAAATGGTGCAA+TGG 0.690674 4_2:-55294970 None:intergenic
CCATTGTTAAAAGATGCAAG+TGG 0.691122 4_2:+55293694 Msa0608280:CDS
GTTCTCCGTTAGTTACGGCG+AGG 0.691247 4_2:+55293522 Msa0608280:CDS
CATTCATGGCAGGTTGACGG+CGG 0.701624 4_2:-55297587 None:intergenic
CCACTGTTGTCAGAGGTGGT+GGG 0.703169 4_2:-55296569 None:intergenic
CAAAATGAAGGATCTTGAGG+TGG 0.718294 4_2:-55295090 None:intergenic
CCCACCACCTCTGACAACAG+TGG 0.721504 4_2:+55296569 Msa0608280:CDS
TGCAATGGTGCCATATGAGG+AGG 0.727370 4_2:-55294955 None:intergenic
ATTGGAGAACCCCAGCAAAG+CGG 0.728169 4_2:+55293570 Msa0608280:CDS
TCTGCTTTACACATGCCATG+GGG 0.759671 4_2:-55296876 None:intergenic
ACAGCTAGGAGTGGACAAGG+CGG 0.769326 4_2:+55294495 Msa0608280:CDS
TGCTTGCAGTGTCACCACTG+AGG 0.812976 4_2:+55293302 Msa0608280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTTTTTAAAAAAAA+GGG - chr4_2:55297397-55297416 None:intergenic 0.0%
!!! TAATTTTTTTTTTAAAAAAA+AGG - chr4_2:55297398-55297417 None:intergenic 0.0%
!!! TTTTTATAGCATTTTTTTTT+CGG + chr4_2:55294107-55294126 Msa0608280:intron 10.0%
!!! TATTGCTATTATTATTAAAT+AGG + chr4_2:55294273-55294292 Msa0608280:intron 10.0%
!!! CTAATAATTAAGATTTTTTT+TGG + chr4_2:55295198-55295217 Msa0608280:intron 10.0%
!! ATAAAAACAAATTATTGTTT+AGG + chr4_2:55295304-55295323 Msa0608280:intron 10.0%
!! AAAAAATTTATATATGAGAT+CGG + chr4_2:55295401-55295420 Msa0608280:intron 10.0%
!!! TCTCTTTTAATATTTTTTTT+GGG - chr4_2:55295516-55295535 None:intergenic 10.0%
!!! TTCTCTTTTAATATTTTTTT+TGG - chr4_2:55295517-55295536 None:intergenic 10.0%
!! AACCTTAAATAAATATTATT+TGG - chr4_2:55295612-55295631 None:intergenic 10.0%
!! ATAATAACTAAATATCATTT+GGG - chr4_2:55295945-55295964 None:intergenic 10.0%
!! TATAATAACTAAATATCATT+TGG - chr4_2:55295946-55295965 None:intergenic 10.0%
!! TTTGTCATATATTATATATA+AGG + chr4_2:55296105-55296124 Msa0608280:intron 10.0%
!!! CAATTTTTTTTTTTTTTTGA+GGG - chr4_2:55296253-55296272 None:intergenic 10.0%
!!! TCAATTTTTTTTTTTTTTTG+AGG - chr4_2:55296254-55296273 None:intergenic 10.0%
!! ATATAAATTGTGAGTATATT+TGG - chr4_2:55294245-55294264 None:intergenic 15.0%
!!! TTTTATTATTGCAGAAATAT+AGG + chr4_2:55294785-55294804 Msa0608280:intron 15.0%
!! TATACAATGATAGATATATA+GGG - chr4_2:55295172-55295191 None:intergenic 15.0%
!!! ACAATTAAGATTTTAAACTT+TGG + chr4_2:55295231-55295250 Msa0608280:intron 15.0%
!!! TAGGAAAATATACAATTTTT+AGG + chr4_2:55295437-55295456 Msa0608280:intron 15.0%
!! GTCCAAATAATATTTATTTA+AGG + chr4_2:55295607-55295626 Msa0608280:intron 15.0%
!! CAAATGGTATATATAAAAAT+TGG - chr4_2:55295672-55295691 None:intergenic 15.0%
!! TAATAACTAAATATCATTTG+GGG - chr4_2:55295944-55295963 None:intergenic 15.0%
!! ATATATTATATATAAGGCAT+GGG + chr4_2:55296111-55296130 Msa0608280:intron 15.0%
!!! TTGTTTTAAAATCTTTTTCT+AGG - chr4_2:55296169-55296188 None:intergenic 15.0%
!!! ATGTGAAACTTTTTAATATA+TGG - chr4_2:55297033-55297052 None:intergenic 15.0%
!!! ATTATAAGATAGTTTTGTGA+AGG - chr4_2:55293109-55293128 None:intergenic 20.0%
!!! ACTAGGTGAATTATTATTTT+TGG - chr4_2:55293207-55293226 None:intergenic 20.0%
!! GAAACTCAATATATAAAACT+AGG - chr4_2:55293224-55293243 None:intergenic 20.0%
!! TATTAAAAAAATGTTAGCAG+TGG + chr4_2:55294071-55294090 Msa0608280:intron 20.0%
!! TGTTAACAATTCAAATTTAC+CGG + chr4_2:55294133-55294152 Msa0608280:intron 20.0%
!! CTATACAATGATAGATATAT+AGG - chr4_2:55295173-55295192 None:intergenic 20.0%
!! GTACAAACTGAAATAATTAA+GGG - chr4_2:55295267-55295286 None:intergenic 20.0%
!! AGTACAAACTGAAATAATTA+AGG - chr4_2:55295268-55295287 None:intergenic 20.0%
!!! GAAAATATACAATTTTTAGG+AGG + chr4_2:55295440-55295459 Msa0608280:intron 20.0%
!!! TTTTGAATATAACGAACAAA+TGG - chr4_2:55295688-55295707 None:intergenic 20.0%
!!! TTTGTCGACTTTTATATTTT+AGG + chr4_2:55295780-55295799 Msa0608280:intron 20.0%
!! CATATATTATATATAAGGCA+TGG + chr4_2:55296110-55296129 Msa0608280:intron 20.0%
!! TTGTTATAAATCAAAATTCG+AGG + chr4_2:55296374-55296393 Msa0608280:intron 20.0%
!!! ATTACATCACTTTACTATTT+TGG + chr4_2:55296601-55296620 Msa0608280:intron 20.0%
!!! TGTCAATCTTATCTTTAAAA+AGG - chr4_2:55296663-55296682 None:intergenic 20.0%
!! TGTGCTATATTTATATATGT+TGG - chr4_2:55296715-55296734 None:intergenic 20.0%
!! TTATTCTCCAAATATAAGTT+TGG + chr4_2:55296785-55296804 Msa0608280:intron 20.0%
!!! TGTTATGTTATTTTCCTTTA+TGG - chr4_2:55296815-55296834 None:intergenic 20.0%
!!! AATTCTCTTTATTTAGTTTC+CGG + chr4_2:55297007-55297026 Msa0608280:intron 20.0%
!!! CTTTTAGGTGTTTTTATATT+GGG - chr4_2:55297139-55297158 None:intergenic 20.0%
!!! ATGATTTATGTTGCCATTTT+TGG - chr4_2:55293024-55293043 None:intergenic 25.0%
!! TGTTTAAATAATGGAGAGTT+TGG - chr4_2:55293072-55293091 None:intergenic 25.0%
!!! TTTTGAGAAAAGCTTAGAAA+TGG - chr4_2:55293142-55293161 None:intergenic 25.0%
! TATATATTGAGTTTCCCATT+TGG + chr4_2:55293229-55293248 Msa0608280:five_prime_UTR 25.0%
! CCTTCTAATAATAACCAAAT+GGG - chr4_2:55293246-55293265 None:intergenic 25.0%
! ACCTTCTAATAATAACCAAA+TGG - chr4_2:55293247-55293266 None:intergenic 25.0%
! GAATTCAAAGAAAGATTATG+AGG + chr4_2:55293600-55293619 Msa0608280:CDS 25.0%
! AGTCAAGGTATACTTAAATA+AGG + chr4_2:55293768-55293787 Msa0608280:intron 25.0%
! CTAAGTGGTTTATCTTAATT+TGG + chr4_2:55293813-55293832 Msa0608280:intron 25.0%
!! TTGTCTCTATAATTTGTCAA+GGG - chr4_2:55293922-55293941 None:intergenic 25.0%
!! TTTGTCTCTATAATTTGTCA+AGG - chr4_2:55293923-55293942 None:intergenic 25.0%
! TTTGCTTATTCATCGTAATT+AGG + chr4_2:55294196-55294215 Msa0608280:intron 25.0%
! AATTTGCATATTACATGTCT+TGG + chr4_2:55294313-55294332 Msa0608280:intron 25.0%
!!! CACTATTTATCAAGTTTTGA+TGG + chr4_2:55294721-55294740 Msa0608280:intron 25.0%
! TGATAGATATATAGGGAATT+TGG - chr4_2:55295165-55295184 None:intergenic 25.0%
!! TTTTTATATATGAAACCAGC+GGG - chr4_2:55295291-55295310 None:intergenic 25.0%
!! GTTTTTATATATGAAACCAG+CGG - chr4_2:55295292-55295311 None:intergenic 25.0%
! AAATTTATATATGAGATCGG+AGG + chr4_2:55295404-55295423 Msa0608280:intron 25.0%
! AATTTATATATGAGATCGGA+GGG + chr4_2:55295405-55295424 Msa0608280:intron 25.0%
!!! GTGCTCAAATATTGTTATTT+GGG - chr4_2:55295485-55295504 None:intergenic 25.0%
!! TGTGCTCAAATATTGTTATT+TGG - chr4_2:55295486-55295505 None:intergenic 25.0%
! AACAAGGATTCAATTATATG+TGG - chr4_2:55296230-55296249 None:intergenic 25.0%
!!! TTTTTTTTTTTGAGGGAACA+AGG - chr4_2:55296246-55296265 None:intergenic 25.0%
!! GAAACTTTTTAATATATGGC+CGG - chr4_2:55297029-55297048 None:intergenic 25.0%
!!! GCTTTTAGGTGTTTTTATAT+TGG - chr4_2:55297140-55297159 None:intergenic 25.0%
!!! AGATGTTTTTATCAAAACCA+TGG + chr4_2:55297267-55297286 Msa0608280:CDS 25.0%
GAGAACGTGTGTTTAAATAA+TGG - chr4_2:55293081-55293100 None:intergenic 30.0%
AGAAAAGCTTAGAAATGGTA+GGG - chr4_2:55293137-55293156 None:intergenic 30.0%
CCCATTTGGTTATTATTAGA+AGG + chr4_2:55293243-55293262 Msa0608280:five_prime_UTR 30.0%
TGAGGATAAAACAAGTTGTT+CGG + chr4_2:55293618-55293637 Msa0608280:CDS 30.0%
TTAAAAGATGCAAGTGGAAA+AGG + chr4_2:55293700-55293719 Msa0608280:CDS 30.0%
AGTAAATCTAAGAATCCTCA+AGG + chr4_2:55293736-55293755 Msa0608280:CDS 30.0%
GTAAATCTAAGAATCCTCAA+GGG + chr4_2:55293737-55293756 Msa0608280:CDS 30.0%
!!! AGAGTTTTATTCTTGCTAAG+TGG + chr4_2:55293798-55293817 Msa0608280:intron 30.0%
!!! CAGTACTAACTTTTTAGCTA+AGG + chr4_2:55293871-55293890 Msa0608280:intron 30.0%
TTTATAGTGCGTTGTATGTT+TGG + chr4_2:55293994-55294013 Msa0608280:intron 30.0%
! TTATCATGAGAGAAGTATAC+CGG - chr4_2:55294155-55294174 None:intergenic 30.0%
AATTAGTGTGTTTATTGTGC+AGG + chr4_2:55294426-55294445 Msa0608280:intron 30.0%
ATAATCTCCAAAATCCTTGA+AGG - chr4_2:55294523-55294542 None:intergenic 30.0%
!! TTCAAGGATTTTGGAGATTA+TGG + chr4_2:55294522-55294541 Msa0608280:CDS 30.0%
!! TCAAGGATTTTGGAGATTAT+GGG + chr4_2:55294523-55294542 Msa0608280:CDS 30.0%
TGAAATTCATATGTACCTGA+AGG - chr4_2:55294585-55294604 None:intergenic 30.0%
AATTTCAAGAGCTAGCATAT+AGG + chr4_2:55294598-55294617 Msa0608280:intron 30.0%
AATCAAAGGAGACAAGTATA+TGG + chr4_2:55294856-55294875 Msa0608280:CDS 30.0%
ATACATGTAAGGGTCTAAAA+TGG - chr4_2:55294981-55295000 None:intergenic 30.0%
! ATTTTAGACCCTTACATGTA+TGG + chr4_2:55294980-55294999 Msa0608280:CDS 30.0%
! TTTTAGACCCTTACATGTAT+GGG + chr4_2:55294981-55295000 Msa0608280:CDS 30.0%
AAATTAGAGAAAGTCTTTCG+TGG - chr4_2:55295639-55295658 None:intergenic 30.0%
AGGATTCAATTATATGTGGT+TGG - chr4_2:55296226-55296245 None:intergenic 30.0%
! CATTTTAGATACACGAATCA+AGG - chr4_2:55296477-55296496 None:intergenic 30.0%
! TTGTCTTCTACTCTGAATTT+TGG + chr4_2:55296635-55296654 Msa0608280:intron 30.0%
TGAATGCATGTATGTATGTA+TGG - chr4_2:55296971-55296990 None:intergenic 30.0%
! AAGAGCATGTGTTTATAAGT+TGG + chr4_2:55297068-55297087 Msa0608280:intron 30.0%
AGAGCATGTGTTTATAAGTT+GGG + chr4_2:55297069-55297088 Msa0608280:intron 30.0%
! TTGAAATCTATGGAGCTTTT+AGG - chr4_2:55297154-55297173 None:intergenic 30.0%
TAAAAGCTCCATAGATTTCA+AGG + chr4_2:55297153-55297172 Msa0608280:intron 30.0%
TTATATAACATCACCCAATC+AGG + chr4_2:55297354-55297373 Msa0608280:three_prime_UTR 30.0%
TATATAACATCACCCAATCA+GGG + chr4_2:55297355-55297374 Msa0608280:three_prime_UTR 30.0%
!!! TAAGTTTGTTTTGAAGTGGA+AGG - chr4_2:55297514-55297533 None:intergenic 30.0%
!!! TGAGTAAGTTTGTTTTGAAG+TGG - chr4_2:55297518-55297537 None:intergenic 30.0%
!!! TATGTTGCCATTTTTGGAAG+TGG - chr4_2:55293018-55293037 None:intergenic 35.0%
GAGAAAAGCTTAGAAATGGT+AGG - chr4_2:55293138-55293157 None:intergenic 35.0%
AAAAACTCTCCATCTCTTCT+TGG - chr4_2:55293348-55293367 None:intergenic 35.0%
CCAATAAATTTCCTTCCGAA+AGG - chr4_2:55293411-55293430 None:intergenic 35.0%
CCTTTCGGAAGGAAATTTAT+TGG + chr4_2:55293408-55293427 Msa0608280:CDS 35.0%
GAGGATAAAACAAGTTGTTC+GGG + chr4_2:55293619-55293638 Msa0608280:CDS 35.0%
GAGTAAAAGAGATGAATCTG+TGG + chr4_2:55293663-55293682 Msa0608280:CDS 35.0%
! CCACTTGCATCTTTTAACAA+TGG - chr4_2:55293697-55293716 None:intergenic 35.0%
CCATTGTTAAAAGATGCAAG+TGG + chr4_2:55293694-55293713 Msa0608280:CDS 35.0%
AGCTAGCATATAGGAGAAAA+AGG + chr4_2:55294607-55294626 Msa0608280:intron 35.0%
TTTAGACCCTTACATGTATG+GGG + chr4_2:55294982-55295001 Msa0608280:CDS 35.0%
! AAAGACTGGTCCATATTTGA+AGG - chr4_2:55295012-55295031 None:intergenic 35.0%
!!! AATTTGGCTGCTATTTTCAG+TGG - chr4_2:55295149-55295168 None:intergenic 35.0%
!! TTTTAGGAGGAACACTTACT+TGG + chr4_2:55295453-55295472 Msa0608280:intron 35.0%
TTTAGGAGGAACACTTACTT+GGG + chr4_2:55295454-55295473 Msa0608280:intron 35.0%
TCGATATGTACAAGTACGTT+TGG - chr4_2:55296031-55296050 None:intergenic 35.0%
TGGTCAGCCAAACTTATATT+TGG - chr4_2:55296795-55296814 None:intergenic 35.0%
ATAAGTTTGGCTGACCATAA+AGG + chr4_2:55296798-55296817 Msa0608280:intron 35.0%
TACTCATGCCTTGAAATCTA+TGG - chr4_2:55297164-55297183 None:intergenic 35.0%
TTTCTTCAAGTGCATGATGA+GGG - chr4_2:55297299-55297318 None:intergenic 35.0%
! TTTTCTTCAAGTGCATGATG+AGG - chr4_2:55297300-55297319 None:intergenic 35.0%
TCATCATGCACTTGAAGAAA+AGG + chr4_2:55297299-55297318 Msa0608280:CDS 35.0%
! ACTGATGATGATGATGATGA+TGG - chr4_2:55297541-55297560 None:intergenic 35.0%
ACCTTATGATGACACATTCA+TGG - chr4_2:55297604-55297623 None:intergenic 35.0%
TCCTTCTCTACATACTCTCT+TGG + chr4_2:55292990-55293009 Msa0608280:five_prime_UTR 40.0%
CTCTTCTTGGTTTCCATCTT+TGG - chr4_2:55293335-55293354 None:intergenic 40.0%
AGATGGAAACCAAGAAGAGA+TGG + chr4_2:55293336-55293355 Msa0608280:CDS 40.0%
!! AGAGATGGAGAGTTTTTCGA+TGG + chr4_2:55293351-55293370 Msa0608280:CDS 40.0%
!! GAGATGGAGAGTTTTTCGAT+GGG + chr4_2:55293352-55293371 Msa0608280:CDS 40.0%
!! AGATGGAGAGTTTTTCGATG+GGG + chr4_2:55293353-55293372 Msa0608280:CDS 40.0%
! GGGAGTACAACAATTACAAC+CGG + chr4_2:55293373-55293392 Msa0608280:CDS 40.0%
AACTTCGACGACTTCTTTGT+TGG + chr4_2:55293439-55293458 Msa0608280:CDS 40.0%
GGAATCAGAGATGAATTCCT+CGG + chr4_2:55293543-55293562 Msa0608280:CDS 40.0%
AAAACAAGTTGTTCGGGTCA+AGG + chr4_2:55293625-55293644 Msa0608280:CDS 40.0%
! TTGACTTTTCTCTTCCCTTG+AGG - chr4_2:55293754-55293773 None:intergenic 40.0%
TCAAGGGAAGAGAAAAGTCA+AGG + chr4_2:55293753-55293772 Msa0608280:CDS 40.0%
GCAACTGTTCAAAATTTCGC+TGG + chr4_2:55293960-55293979 Msa0608280:intron 40.0%
TAGGATCTTCATGTCTAACG+AGG + chr4_2:55294215-55294234 Msa0608280:intron 40.0%
!! AGTGTCTCGTGCACTTTTTA+AGG - chr4_2:55294386-55294405 None:intergenic 40.0%
TAGTGTGTTTATTGTGCAGG+TGG + chr4_2:55294429-55294448 Msa0608280:intron 40.0%
TGTTTATTGTGCAGGTGGAT+TGG + chr4_2:55294434-55294453 Msa0608280:intron 40.0%
GAAGGTGACTAGCAATGTTA+TGG - chr4_2:55294567-55294586 None:intergenic 40.0%
TAACATTGCTAGTCACCTTC+AGG + chr4_2:55294567-55294586 Msa0608280:CDS 40.0%
GTACTCGTACGATGTCTTTA+TGG + chr4_2:55294674-55294693 Msa0608280:intron 40.0%
! AAGCAGCAAGTTGGAATCAA+AGG + chr4_2:55294842-55294861 Msa0608280:CDS 40.0%
CAAAGGAGACAAGTATATGG+TGG + chr4_2:55294859-55294878 Msa0608280:CDS 40.0%
! AGACAAGTATATGGTGGTGT+AGG + chr4_2:55294865-55294884 Msa0608280:CDS 40.0%
! GACAAGTATATGGTGGTGTA+GGG + chr4_2:55294866-55294885 Msa0608280:CDS 40.0%
! ACAAGTATATGGTGGTGTAG+GGG + chr4_2:55294867-55294886 Msa0608280:CDS 40.0%
! AAGGGTCTAAAATGGTGCAA+TGG - chr4_2:55294973-55294992 None:intergenic 40.0%
ATGGGGACATCCTTCAAATA+TGG + chr4_2:55294999-55295018 Msa0608280:CDS 40.0%
CAAAATGAAGGATCTTGAGG+TGG - chr4_2:55295093-55295112 None:intergenic 40.0%
! CACCTCAAGATCCTTCATTT+TGG + chr4_2:55295091-55295110 Msa0608280:CDS 40.0%
TGCCAAAATGAAGGATCTTG+AGG - chr4_2:55295096-55295115 None:intergenic 40.0%
TGGTGAGAATGCCAAAATGA+AGG - chr4_2:55295105-55295124 None:intergenic 40.0%
CTCCAATAAACGCAATGATC+CGG + chr4_2:55296529-55296548 Msa0608280:CDS 40.0%
ATAGTTACCACTGTTGTCAG+AGG - chr4_2:55296579-55296598 None:intergenic 40.0%
AACATGCAGAGACCATCATT+TGG + chr4_2:55296831-55296850 Msa0608280:intron 40.0%
ATCTGCTTTACACATGCCAT+GGG - chr4_2:55296880-55296899 None:intergenic 40.0%
CATGTATGTATGTATGGCGA+AGG - chr4_2:55296965-55296984 None:intergenic 40.0%
AACTCACTATGTCACACAAG+TGG - chr4_2:55297328-55297347 None:intergenic 40.0%
! CGTTTTCGTTTTCGTTTCAC+TGG - chr4_2:55297468-55297487 None:intergenic 40.0%
!!! GTTTGTTTTGAAGTGGAAGG+AGG - chr4_2:55297511-55297530 None:intergenic 40.0%
TATGATGACACATTCATGGC+AGG - chr4_2:55297600-55297619 None:intergenic 40.0%
GCCATGAATGTGTCATCATA+AGG + chr4_2:55297600-55297619 Msa0608280:three_prime_UTR 40.0%
! GCCAAGAGAGTATGTAGAGA+AGG - chr4_2:55292994-55293013 None:intergenic 45.0%
CTTGGCACCACTTCCAAAAA+TGG + chr4_2:55293008-55293027 Msa0608280:five_prime_UTR 45.0%
GAAGGTTGAGTACTTGAGTG+TGG + chr4_2:55293261-55293280 Msa0608280:five_prime_UTR 45.0%
!! TCTTTGGTAGTGTTCCTCAG+TGG - chr4_2:55293319-55293338 None:intergenic 45.0%
CTGAGGAACACTACCAAAGA+TGG + chr4_2:55293319-55293338 Msa0608280:CDS 45.0%
ACAATTACAACCGGCGACTT+CGG + chr4_2:55293382-55293401 Msa0608280:CDS 45.0%
TTTGTTGGCATCAACGTCGA+TGG + chr4_2:55293454-55293473 Msa0608280:CDS 45.0%
TCTGGATCCATCTCCAAATC+CGG - chr4_2:55293490-55293509 None:intergenic 45.0%
GAGAACTCAGCAAACATCTC+TGG - chr4_2:55293508-55293527 None:intergenic 45.0%
GCTGAGTTCTCCGTTAGTTA+CGG + chr4_2:55293517-55293536 Msa0608280:CDS 45.0%
TGATTCCTCGCCGTAACTAA+CGG - chr4_2:55293530-55293549 None:intergenic 45.0%
TTCTTCTCTTCCGCTTTGCT+GGG - chr4_2:55293583-55293602 None:intergenic 45.0%
ATTCTTCTCTTCCGCTTTGC+TGG - chr4_2:55293584-55293603 None:intergenic 45.0%
ACAAATCTCCTGTGCAAGTC+TGG - chr4_2:55294463-55294482 None:intergenic 45.0%
AGAGAGAAGTGATCAGTCCA+TGG + chr4_2:55294893-55294912 Msa0608280:CDS 45.0%
GGATGTCCCCATACATGTAA+GGG - chr4_2:55294991-55295010 None:intergenic 45.0%
AGGATGTCCCCATACATGTA+AGG - chr4_2:55294992-55295011 None:intergenic 45.0%
! ACCAGTCTTTTCTGCACATG+TGG + chr4_2:55295022-55295041 Msa0608280:CDS 45.0%
AATGAAGGATCTTGAGGTGG+TGG - chr4_2:55295090-55295109 None:intergenic 45.0%
ATTTCAGTTTGTACTCCCGC+TGG + chr4_2:55295273-55295292 Msa0608280:intron 45.0%
!! GATCGGAGGGAGTATTTGAT+AGG + chr4_2:55295418-55295437 Msa0608280:intron 45.0%
AGAGTAGAAGACAACGTGCA+TGG - chr4_2:55296629-55296648 None:intergenic 45.0%
GGGATTGGACATCCAAATGA+TGG - chr4_2:55296846-55296865 None:intergenic 45.0%
TCATTTGGATGTCCAATCCC+CGG + chr4_2:55296846-55296865 Msa0608280:CDS 45.0%
TCTGCTTTACACATGCCATG+GGG - chr4_2:55296879-55296898 None:intergenic 45.0%
GATCTGCTTTACACATGCCA+TGG - chr4_2:55296881-55296900 None:intergenic 45.0%
GGCATGTGTAAAGCAGATCA+CGG + chr4_2:55296882-55296901 Msa0608280:CDS 45.0%
!! GAAGGAAATTCAGTACCGGT+GGG - chr4_2:55296947-55296966 None:intergenic 45.0%
TGGCGAAGGAAATTCAGTAC+CGG - chr4_2:55296951-55296970 None:intergenic 45.0%
! AAAAAAAAGGGTGTGCCACG+TGG - chr4_2:55297385-55297404 None:intergenic 45.0%
TAAGAGAACACGTGAGTGAG+AGG + chr4_2:55297431-55297450 Msa0608280:three_prime_UTR 45.0%
AAGAGAACACGTGAGTGAGA+GGG + chr4_2:55297432-55297451 Msa0608280:three_prime_UTR 45.0%
! ATCAGTTTTCCGTCCACCAT+CGG + chr4_2:55297555-55297574 Msa0608280:three_prime_UTR 45.0%
! ACACATTCATGGCAGGTTGA+CGG - chr4_2:55297593-55297612 None:intergenic 45.0%
!! TTATTTAACTATTAAATTAA+AGG + chr4_2:55296005-55296024 Msa0608280:intron 5.0%
TGGAGACATCTTGCCGGATT+TGG + chr4_2:55293474-55293493 Msa0608280:CDS 50.0%
CATCTTGCCGGATTTGGAGA+TGG + chr4_2:55293480-55293499 Msa0608280:CDS 50.0%
GATGAATTCCTCGGCGTCAT+TGG + chr4_2:55293552-55293571 Msa0608280:CDS 50.0%
ATTGGAGAACCCCAGCAAAG+CGG + chr4_2:55293570-55293589 Msa0608280:CDS 50.0%
TCTTCTCTTCCGCTTTGCTG+GGG - chr4_2:55293582-55293601 None:intergenic 50.0%
AAGTTGTTCGGGTCAAGGCT+CGG + chr4_2:55293630-55293649 Msa0608280:CDS 50.0%
ATTGGACACCAGACTTGCAC+AGG + chr4_2:55294452-55294471 Msa0608280:CDS 50.0%
CAGGAGATTTGTGCAAGCTG+TGG + chr4_2:55294471-55294490 Msa0608280:CDS 50.0%
GTGAAGCAGAAGCAGCAAGT+TGG + chr4_2:55294833-55294852 Msa0608280:CDS 50.0%
TATGAGGAGGAGGAGGATGA+GGG - chr4_2:55294945-55294964 None:intergenic 50.0%
ATATGAGGAGGAGGAGGATG+AGG - chr4_2:55294946-55294965 None:intergenic 50.0%
TCATCCTCCTCCTCCTCATA+TGG + chr4_2:55294945-55294964 Msa0608280:CDS 50.0%
!! AATGGTGCCATATGAGGAGG+AGG - chr4_2:55294955-55294974 None:intergenic 50.0%
!! TGCAATGGTGCCATATGAGG+AGG - chr4_2:55294958-55294977 None:intergenic 50.0%
!! TGGTGCAATGGTGCCATATG+AGG - chr4_2:55294961-55294980 None:intergenic 50.0%
GCCACATGTGCAGAAAAGAC+TGG - chr4_2:55295026-55295045 None:intergenic 50.0%
GGCCGGATCATTGCGTTTAT+TGG - chr4_2:55296534-55296553 None:intergenic 50.0%
ATAAACGCAATGATCCGGCC+AGG + chr4_2:55296534-55296553 Msa0608280:CDS 50.0%
GGAAACAATGTGTTCCTGGC+CGG - chr4_2:55296551-55296570 None:intergenic 50.0%
GGTGGGAAACAATGTGTTCC+TGG - chr4_2:55296555-55296574 None:intergenic 50.0%
GTTACCACTGTTGTCAGAGG+TGG - chr4_2:55296576-55296595 None:intergenic 50.0%
CTTTACACATGCCATGGGGT+GGG - chr4_2:55296875-55296894 None:intergenic 50.0%
TGGGAAGTCGAAGAAGTGAC+CGG - chr4_2:55296928-55296947 None:intergenic 50.0%
TCACTTCTTCGACTTCCCAC+CGG + chr4_2:55296929-55296948 Msa0608280:CDS 50.0%
! CGAAGGAAATTCAGTACCGG+TGG - chr4_2:55296948-55296967 None:intergenic 50.0%
! GAGAGCATAGACGCAGCTAT+TGG + chr4_2:55297244-55297263 Msa0608280:CDS 50.0%
CTTCAAGTGCATGATGAGGG+TGG - chr4_2:55297296-55297315 None:intergenic 50.0%
GGAAGGAGGAAGCAGAATGT+TGG - chr4_2:55297497-55297516 None:intergenic 50.0%
TGCTTGCAGTGTCACCACTG+AGG + chr4_2:55293302-55293321 Msa0608280:CDS 55.0%
GACTTCGGTGACCTTTCGGA+AGG + chr4_2:55293397-55293416 Msa0608280:CDS 55.0%
CGTCGATGGAGACATCTTGC+CGG + chr4_2:55293468-55293487 Msa0608280:CDS 55.0%
GTTCTCCGTTAGTTACGGCG+AGG + chr4_2:55293522-55293541 Msa0608280:CDS 55.0%
AAGTGCACGAGACACTCGTC+AGG + chr4_2:55294390-55294409 Msa0608280:intron 55.0%
GTGCAAGCTGTGGAACAGCT+AGG + chr4_2:55294481-55294500 Msa0608280:CDS 55.0%
AGCTGTGGAACAGCTAGGAG+TGG + chr4_2:55294486-55294505 Msa0608280:CDS 55.0%
GGAACAGCTAGGAGTGGACA+AGG + chr4_2:55294492-55294511 Msa0608280:CDS 55.0%
ACAGCTAGGAGTGGACAAGG+CGG + chr4_2:55294495-55294514 Msa0608280:CDS 55.0%
! GGCGGTGCCTTCAAGGATTT+TGG + chr4_2:55294513-55294532 Msa0608280:CDS 55.0%
ATGAGGAGGAGGAGGATGAG+GGG - chr4_2:55294944-55294963 None:intergenic 55.0%
GCCACTCTCATGGACACCAT+CGG + chr4_2:55295056-55295075 Msa0608280:CDS 55.0%
ACCGATGGTGTCCATGAGAG+TGG - chr4_2:55295060-55295079 None:intergenic 55.0%
! GAAGGATCTTGAGGTGGTGG+AGG - chr4_2:55295087-55295106 None:intergenic 55.0%
TTGGCATTCTCACCACCAGC+AGG + chr4_2:55295110-55295129 Msa0608280:CDS 55.0%
TAGAGAGAGCTACCTGCTGG+TGG - chr4_2:55295125-55295144 None:intergenic 55.0%
GGTTAGAGAGAGCTACCTGC+TGG - chr4_2:55295128-55295147 None:intergenic 55.0%
CCACTGTTGTCAGAGGTGGT+GGG - chr4_2:55296572-55296591 None:intergenic 55.0%
ACCACTGTTGTCAGAGGTGG+TGG - chr4_2:55296573-55296592 None:intergenic 55.0%
GCTTTACACATGCCATGGGG+TGG - chr4_2:55296876-55296895 None:intergenic 55.0%
ATTAGCACCCTTCCTGCTCC+CGG + chr4_2:55296906-55296925 Msa0608280:CDS 55.0%
GGGAAGTCGAAGAAGTGACC+GGG - chr4_2:55296927-55296946 None:intergenic 55.0%
CATGATGAGGGTGGCAACCA+TGG - chr4_2:55297287-55297306 None:intergenic 55.0%
! CATTCATGGCAGGTTGACGG+CGG - chr4_2:55297590-55297609 None:intergenic 55.0%
CGAAAGGTCACCGAAGTCGC+CGG - chr4_2:55293395-55293414 None:intergenic 60.0%
CGGCGACTTCGGTGACCTTT+CGG + chr4_2:55293393-55293412 Msa0608280:CDS 60.0%
TGGACAAGGCGGTGCCTTCA+AGG + chr4_2:55294506-55294525 Msa0608280:CDS 60.0%
AGGATGAGGGGGGAAACCCA+CGG - chr4_2:55294932-55294951 None:intergenic 60.0%
TGAGGAGGAGGAGGATGAGG+GGG - chr4_2:55294943-55294962 None:intergenic 60.0%
! GGTGCCATATGAGGAGGAGG+AGG - chr4_2:55294952-55294971 None:intergenic 60.0%
CATGAGAGTGGCTGCGGTGT+AGG - chr4_2:55295048-55295067 None:intergenic 60.0%
CTACACCGCAGCCACTCTCA+TGG + chr4_2:55295046-55295065 Msa0608280:CDS 60.0%
GGTGTCCATGAGAGTGGCTG+CGG - chr4_2:55295054-55295073 None:intergenic 60.0%
CCCACCACCTCTGACAACAG+TGG + chr4_2:55296569-55296588 Msa0608280:CDS 60.0%
GAAGTGACCGGGAGCAGGAA+GGG - chr4_2:55296916-55296935 None:intergenic 60.0%
AGAAGTGACCGGGAGCAGGA+AGG - chr4_2:55296917-55296936 None:intergenic 60.0%
TCGAAGAAGTGACCGGGAGC+AGG - chr4_2:55296921-55296940 None:intergenic 60.0%
CCACGTGGCACACCCTGATT+GGG - chr4_2:55297370-55297389 None:intergenic 60.0%
GGGGTTCTCCAATGACGCCG+AGG - chr4_2:55293563-55293582 None:intergenic 65.0%
AAACCCACGGTGGGTGCAGC+TGG - chr4_2:55294919-55294938 None:intergenic 65.0%
TGAGGGGGGAAACCCACGGT+GGG - chr4_2:55294928-55294947 None:intergenic 65.0%
ATGAGGGGGGAAACCCACGG+TGG - chr4_2:55294929-55294948 None:intergenic 65.0%
GAGGAGGAGGAGGATGAGGG+GGG - chr4_2:55294942-55294961 None:intergenic 65.0%
!! GGTGGTGGAGGAGCTACCGA+TGG - chr4_2:55295075-55295094 None:intergenic 65.0%
ATGCCATGGGGTGGGATGCC+GGG - chr4_2:55296867-55296886 None:intergenic 65.0%
CATGCCATGGGGTGGGATGC+CGG - chr4_2:55296868-55296887 None:intergenic 65.0%
CCCAATCAGGGTGTGCCACG+TGG + chr4_2:55297367-55297386 Msa0608280:three_prime_UTR 65.0%
GCCACGTGGCACACCCTGAT+TGG - chr4_2:55297371-55297390 None:intergenic 65.0%
! TCATGGCAGGTTGACGGCGG+CGG - chr4_2:55297587-55297606 None:intergenic 65.0%
ACGGTGGGTGCAGCTGGCCA+TGG - chr4_2:55294913-55294932 None:intergenic 70.0%
ATGGCCAGCTGCACCCACCG+TGG + chr4_2:55294912-55294931 Msa0608280:CDS 70.0%
TGGCCAGCTGCACCCACCGT+GGG + chr4_2:55294913-55294932 Msa0608280:CDS 70.0%
TGGGGTGGGATGCCGGGGAT+TGG - chr4_2:55296861-55296880 None:intergenic 70.0%
ATCCCCGGCATCCCACCCCA+TGG + chr4_2:55296861-55296880 Msa0608280:CDS 70.0%
TGCCATGGGGTGGGATGCCG+GGG - chr4_2:55296866-55296885 None:intergenic 70.0%
!! CGGCGGCGACCGATGGTGGA+CGG - chr4_2:55297567-55297586 None:intergenic 75.0%
! TGGCAGGTTGACGGCGGCGG+CGG - chr4_2:55297584-55297603 None:intergenic 75.0%
! GCGGCGGCGGCGACCGATGG+TGG - chr4_2:55297571-55297590 None:intergenic 85.0%
! ACGGCGGCGGCGGCGACCGA+TGG - chr4_2:55297574-55297593 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 55292978 55297662 55292978 ID=Msa0608280;Name=Msa0608280
chr4_2 mRNA 55292978 55297662 55292978 ID=Msa0608280-mRNA-1;Parent=Msa0608280;Name=Msa0608280-mRNA-1;_AED=0.09;_eAED=0.09;_QI=323|1|1|1|1|1|6|326|406
chr4_2 exon 55292978 55293774 55292978 ID=Msa0608280-mRNA-1:exon:14059;Parent=Msa0608280-mRNA-1
chr4_2 exon 55294448 55294588 55294448 ID=Msa0608280-mRNA-1:exon:14060;Parent=Msa0608280-mRNA-1
chr4_2 exon 55294799 55295131 55294799 ID=Msa0608280-mRNA-1:exon:14061;Parent=Msa0608280-mRNA-1
chr4_2 exon 55296528 55296590 55296528 ID=Msa0608280-mRNA-1:exon:14062;Parent=Msa0608280-mRNA-1
chr4_2 exon 55296840 55296950 55296840 ID=Msa0608280-mRNA-1:exon:14063;Parent=Msa0608280-mRNA-1
chr4_2 exon 55297238 55297662 55297238 ID=Msa0608280-mRNA-1:exon:14064;Parent=Msa0608280-mRNA-1
chr4_2 five_prime_UTR 55292978 55293300 55292978 ID=Msa0608280-mRNA-1:five_prime_utr;Parent=Msa0608280-mRNA-1
chr4_2 CDS 55293301 55293774 55293301 ID=Msa0608280-mRNA-1:cds;Parent=Msa0608280-mRNA-1
chr4_2 CDS 55294448 55294588 55294448 ID=Msa0608280-mRNA-1:cds;Parent=Msa0608280-mRNA-1
chr4_2 CDS 55294799 55295131 55294799 ID=Msa0608280-mRNA-1:cds;Parent=Msa0608280-mRNA-1
chr4_2 CDS 55296528 55296590 55296528 ID=Msa0608280-mRNA-1:cds;Parent=Msa0608280-mRNA-1
chr4_2 CDS 55296840 55296950 55296840 ID=Msa0608280-mRNA-1:cds;Parent=Msa0608280-mRNA-1
chr4_2 CDS 55297238 55297336 55297238 ID=Msa0608280-mRNA-1:cds;Parent=Msa0608280-mRNA-1
chr4_2 three_prime_UTR 55297337 55297662 55297337 ID=Msa0608280-mRNA-1:three_prime_utr;Parent=Msa0608280-mRNA-1
Gene Sequence

>Msa0608280

ATGCTTGCAGTGTCACCACTGAGGAACACTACCAAAGATGGAAACCAAGAAGAGATGGAGAGTTTTTCGATGGGGAGTACAACAATTACAACCGGCGACTTCGGTGACCTTTCGGAAGGAAATTTATTGGAAAGCATCAACTTCGACGACTTCTTTGTTGGCATCAACGTCGATGGAGACATCTTGCCGGATTTGGAGATGGATCCAGAGATGTTTGCTGAGTTCTCCGTTAGTTACGGCGAGGAATCAGAGATGAATTCCTCGGCGTCATTGGAGAACCCCAGCAAAGCGGAAGAGAAGAATTCAAAGAAAGATTATGAGGATAAAACAAGTTGTTCGGGTCAAGGCTCGGAAGAGATAGCGAGTAAAAGAGATGAATCTGTGGTTGTGAATCCATTGTTAAAAGATGCAAGTGGAAAAGGAAGAAAATCATCGAGTAAATCTAAGAATCCTCAAGGGAAGAGAAAAGTCAAGGTGGATTGGACACCAGACTTGCACAGGAGATTTGTGCAAGCTGTGGAACAGCTAGGAGTGGACAAGGCGGTGCCTTCAAGGATTTTGGAGATTATGGGAATTGATTGTCTCACTCGCCATAACATTGCTAGTCACCTTCAGAAATATAGGTCACATAGAAAACATTTGCTAGCGCGTGAAGCAGAAGCAGCAAGTTGGAATCAAAGGAGACAAGTATATGGTGGTGTAGGGGTGAAGAGAGAAGTGATCAGTCCATGGCCAGCTGCACCCACCGTGGGTTTCCCCCCTCATCCTCCTCCTCCTCATATGGCACCATTGCACCATTTTAGACCCTTACATGTATGGGGACATCCTTCAAATATGGACCAGTCTTTTCTGCACATGTGGCCTACACCGCAGCCACTCTCATGGACACCATCGGTAGCTCCTCCACCACCTCAAGATCCTTCATTTTGGCATTCTCACCACCAGCAGACTCCAATAAACGCAATGATCCGGCCAGGAACACATTGTTTCCCACCACCTCTGACAACAGTGAGACCATCATTTGGATGTCCAATCCCCGGCATCCCACCCCATGGCATGTGTAAAGCAGATCACGGCATTAGCACCCTTCCTGCTCCCGGTCACTTCTTCGACTTCCCACCGTCAAATGAGAGCATAGACGCAGCTATTGGAGATGTTTTTATCAAAACCATGGTTGCCACCCTCATCATGCACTTGAAGAAAAGGACTCCACTTGTGTGA
Protein sequence

>Msa0608280

MLAVSPLRNTTKDGNQEEMESFSMGSTTITTGDFGDLSEGNLLESINFDDFFVGINVDGDILPDLEMDPEMFAEFSVSYGEESEMNSSASLENPSKAEEKNSKKDYEDKTSCSGQGSEEIASKRDESVVVNPLLKDASGKGRKSSSKSKNPQGKRKVKVDWTPDLHRRFVQAVEQLGVDKAVPSRILEIMGIDCLTRHNIASHLQKYRSHRKHLLAREAEAASWNQRRQVYGGVGVKREVISPWPAAPTVGFPPHPPPPHMAPLHHFRPLHVWGHPSNMDQSFLHMWPTPQPLSWTPSVAPPPPQDPSFWHSHHQQTPINAMIRPGTHCFPPPLTTVRPSFGCPIPGIPPHGMCKADHGISTLPAPGHFFDFPPSNESIDAAIGDVFIKTMVATLIMHLKKRTPLV*