Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1291880 | GAU41458.1 | 63.014 | 438 | 102 | 7 | 97 | 517 | 133 | 527 | 3.97e-176 | 520 |
| Msa1291880 | GAU41458.1 | 84.375 | 64 | 10 | 0 | 56 | 119 | 13 | 76 | 1.98e-26 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1291880 | sp|Q8LPK2|AB2B_ARATH | 68.493 | 219 | 58 | 2 | 97 | 306 | 178 | 394 | 2.71e-89 | 304 |
| Msa1291880 | sp|Q8LPK2|AB2B_ARATH | 62.443 | 221 | 70 | 3 | 305 | 518 | 659 | 873 | 1.60e-76 | 268 |
| Msa1291880 | sp|Q8LPK2|AB2B_ARATH | 54.839 | 124 | 38 | 2 | 484 | 598 | 978 | 1092 | 7.96e-29 | 126 |
| Msa1291880 | sp|Q8LPK2|AB2B_ARATH | 54.128 | 109 | 48 | 1 | 13 | 119 | 13 | 121 | 7.67e-27 | 120 |
| Msa1291880 | sp|Q8LPK2|AB2B_ARATH | 48.936 | 94 | 48 | 0 | 505 | 598 | 370 | 463 | 1.55e-18 | 94.0 |
| Msa1291880 | sp|Q8LPK2|AB2B_ARATH | 27.041 | 196 | 125 | 6 | 131 | 312 | 841 | 1032 | 4.73e-11 | 69.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1291880 | A0A2Z6N9P3 | 63.014 | 438 | 102 | 7 | 97 | 517 | 133 | 527 | 1.90e-176 | 520 |
| Msa1291880 | A0A2Z6N9P3 | 84.375 | 64 | 10 | 0 | 56 | 119 | 13 | 76 | 9.45e-27 | 125 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001950 | Msa1291880 | 0.809139 | 2.192859e-50 | -8.615850e-47 |
| Msa0017940 | Msa1291880 | 0.811177 | 7.977616e-51 | -8.615850e-47 |
| Msa0024680 | Msa1291880 | 0.806787 | 6.941064e-50 | -8.615850e-47 |
| Msa0030310 | Msa1291880 | 0.824344 | 8.491318e-54 | -8.615850e-47 |
| Msa0050580 | Msa1291880 | 0.804108 | 2.530762e-49 | -8.615850e-47 |
| Msa0120330 | Msa1291880 | 0.806083 | 9.771398e-50 | -8.615850e-47 |
| Msa0139900 | Msa1291880 | 0.809932 | 1.482063e-50 | -8.615850e-47 |
| Msa0141830 | Msa1291880 | 0.809339 | 1.987265e-50 | -8.615850e-47 |
| Msa0417630 | Msa1291880 | 0.805550 | 1.264335e-49 | -8.615850e-47 |
| Msa0526700 | Msa1291880 | 0.812295 | 4.555508e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1291880 | MtrunA17_Chr8g0366481 | 92.760 | 221 | 9 | 1 | 305 | 518 | 9 | 229 | 9.36e-135 | 407 |
| Msa1291880 | MtrunA17_Chr8g0366481 | 66.667 | 135 | 45 | 0 | 464 | 598 | 314 | 448 | 5.41e-37 | 145 |
| Msa1291880 | MtrunA17_Chr8g0366481 | 29.714 | 175 | 106 | 5 | 131 | 292 | 197 | 367 | 4.20e-13 | 72.4 |
| Msa1291880 | MtrunA17_Chr8g0366451 | 85.455 | 220 | 21 | 2 | 97 | 307 | 92 | 309 | 7.38e-123 | 370 |
| Msa1291880 | MtrunA17_Chr8g0366451 | 51.042 | 96 | 43 | 2 | 505 | 598 | 284 | 377 | 3.82e-21 | 96.7 |
| Msa1291880 | MtrunA17_Chr5g0410921 | 72.146 | 219 | 50 | 2 | 97 | 306 | 133 | 349 | 3.05e-96 | 319 |
| Msa1291880 | MtrunA17_Chr5g0410921 | 70.270 | 222 | 55 | 3 | 305 | 518 | 614 | 832 | 1.11e-92 | 310 |
| Msa1291880 | MtrunA17_Chr5g0410921 | 61.290 | 124 | 30 | 2 | 484 | 598 | 937 | 1051 | 2.57e-35 | 142 |
| Msa1291880 | MtrunA17_Chr5g0410921 | 80.952 | 63 | 12 | 0 | 57 | 119 | 14 | 76 | 2.28e-26 | 115 |
| Msa1291880 | MtrunA17_Chr5g0410921 | 46.809 | 94 | 50 | 0 | 505 | 598 | 325 | 418 | 1.06e-19 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1291880 | AT4G25960.1 | 68.493 | 219 | 58 | 2 | 97 | 306 | 178 | 394 | 2.76e-90 | 304 |
| Msa1291880 | AT4G25960.1 | 62.443 | 221 | 70 | 3 | 305 | 518 | 659 | 873 | 1.63e-77 | 268 |
| Msa1291880 | AT4G25960.1 | 54.839 | 124 | 38 | 2 | 484 | 598 | 978 | 1092 | 8.09e-30 | 126 |
| Msa1291880 | AT4G25960.1 | 54.128 | 109 | 48 | 1 | 13 | 119 | 13 | 121 | 7.80e-28 | 120 |
| Msa1291880 | AT4G25960.1 | 48.936 | 94 | 48 | 0 | 505 | 598 | 370 | 463 | 1.58e-19 | 94.0 |
| Msa1291880 | AT4G25960.1 | 27.041 | 196 | 125 | 6 | 131 | 312 | 841 | 1032 | 4.81e-12 | 69.7 |
| Msa1291880 | AT1G10680.1 | 66.822 | 214 | 60 | 2 | 97 | 301 | 142 | 353 | 3.87e-84 | 286 |
| Msa1291880 | AT1G10680.1 | 63.590 | 195 | 63 | 2 | 331 | 518 | 632 | 825 | 2.70e-74 | 258 |
| Msa1291880 | AT1G10680.1 | 54.839 | 124 | 38 | 2 | 484 | 598 | 930 | 1044 | 4.52e-32 | 133 |
| Msa1291880 | AT1G10680.1 | 45.000 | 140 | 53 | 2 | 43 | 180 | 9 | 126 | 3.46e-27 | 117 |
Find 336 sgRNAs with CRISPR-Local
Find 1374 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATGAGGTTTGAAGGT+GGG | 0.478182 | 8_3:-47466687 | Msa1291880:five_prime_UTR |
| AAAAGATTCCTGGATGTAGC+TGG | 0.437384 | 8_3:-47465121 | Msa1291880:five_prime_UTR |
| AAAATTGCATTCCTCTTCTG+TGG | 0.510092 | 8_3:-47449460 | Msa1291880:CDS |
| AAAGAGATTAACCGCAACGC+GGG | 0.630148 | 8_3:+47467122 | None:intergenic |
| AAAGATGAGGTTTGAAGGTG+GGG | 0.498168 | 8_3:-47466686 | Msa1291880:five_prime_UTR |
| AAAGTATCTATTGCTTTATA+CGG | 0.245285 | 8_3:-47464915 | Msa1291880:five_prime_UTR |
| AAATACAAACCTTGGCAACT+TGG | 0.422459 | 8_3:+47459428 | None:intergenic |
| AAATGGTTTAAAGGCGTGTG+GGG | 0.577888 | 8_3:-47466956 | Msa1291880:five_prime_UTR |
| AACGAATTCATTTGTTGAAT+AGG | 0.358564 | 8_3:+47467185 | None:intergenic |
| AACTAGGTAAGAAGTTGCAT+GGG | 0.566954 | 8_3:-47466291 | Msa1291880:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTGAGTTATGTTGAAATGA+TGG | - | chr8_3:47447716-47447735 | Msa1291880:intron | 30.0% | |
| TGTTGTTCCAAATGGTTTAA+AGG | - | chr8_3:47447764-47447783 | Msa1291880:intron | 30.0% | |
| TTGATATGTATGGGAAATGT+GGG | - | chr8_3:47447892-47447911 | Msa1291880:intron | 30.0% | |
| ATATCTAGCTATGTGCAATT+TGG | - | chr8_3:47448299-47448318 | Msa1291880:intron | 30.0% | |
| TGTGACATTGTCTTCTTTAT+TGG | - | chr8_3:47448388-47448407 | Msa1291880:CDS | 30.0% | |
| TAACACCTAATTTGATCACA+TGG | - | chr8_3:47448786-47448805 | Msa1291880:intron | 30.0% | |
| GAAGTTATGCTTATGCTATT+AGG | + | chr8_3:47448899-47448918 | None:intergenic | 30.0% | |
| TGGCATTATTGAATTATGGA+AGG | - | chr8_3:47448942-47448961 | Msa1291880:intron | 30.0% | |
| GGCATTATTGAATTATGGAA+GGG | - | chr8_3:47448943-47448962 | Msa1291880:intron | 30.0% | |
| AGCATACATGTCAATAATAG+AGG | + | chr8_3:47449023-47449042 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 47447110 | 47467641 | 47447110 | ID=Msa1291880;Name=Msa1291880 |
| chr8_3 | mRNA | 47447110 | 47467641 | 47447110 | ID=Msa1291880-mRNA-1;Parent=Msa1291880;Name=Msa1291880-mRNA-1;_AED=0.22;_eAED=0.22;_QI=2893|0.2|0.18|1|0.5|0.36|11|0|598 |
| chr8_3 | exon | 47447110 | 47447357 | 47447110 | ID=Msa1291880-mRNA-1:exon:13229;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47448334 | 47448570 | 47448334 | ID=Msa1291880-mRNA-1:exon:13228;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47449359 | 47449601 | 47449359 | ID=Msa1291880-mRNA-1:exon:13227;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47449623 | 47449774 | 47449623 | ID=Msa1291880-mRNA-1:exon:13226;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47454884 | 47454993 | 47454884 | ID=Msa1291880-mRNA-1:exon:13225;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47455172 | 47455417 | 47455172 | ID=Msa1291880-mRNA-1:exon:13224;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47455645 | 47455845 | 47455645 | ID=Msa1291880-mRNA-1:exon:13223;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47459438 | 47459633 | 47459438 | ID=Msa1291880-mRNA-1:exon:13222;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47461992 | 47461998 | 47461992 | ID=Msa1291880-mRNA-1:exon:13221;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47463719 | 47463821 | 47463719 | ID=Msa1291880-mRNA-1:exon:13220;Parent=Msa1291880-mRNA-1 |
| chr8_3 | exon | 47464698 | 47467641 | 47464698 | ID=Msa1291880-mRNA-1:exon:13219;Parent=Msa1291880-mRNA-1 |
| chr8_3 | five_prime_UTR | 47464749 | 47467641 | 47464749 | ID=Msa1291880-mRNA-1:five_prime_utr;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47464698 | 47464748 | 47464698 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47463719 | 47463821 | 47463719 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47461992 | 47461998 | 47461992 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47459438 | 47459633 | 47459438 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47455645 | 47455845 | 47455645 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47455172 | 47455417 | 47455172 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47454884 | 47454993 | 47454884 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47449623 | 47449774 | 47449623 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47449359 | 47449601 | 47449359 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47448334 | 47448570 | 47448334 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| chr8_3 | CDS | 47447110 | 47447357 | 47447110 | ID=Msa1291880-mRNA-1:cds;Parent=Msa1291880-mRNA-1 |
| Gene Sequence |
>Msa1291880 ATGCCTCGTATTATTCCAGATGTTGCTTATCGATCATTTCCTCCATACATTGCAGGACCAAACATTTCTGCATTTATCAGAACAAAGGCAGCAGCTTATCACATTTTTGAAATGATAAATCAAGACATAGTTGGCAAAAATAGCTCAAAAACTGAATTGCAGGGGAGAAAGGTTCCATTGCTCAAGCTTTTCACCTTTGCTGATTTCTATGATTATGTCTTGATGGTCGTTGGATCTGTTGGGGCATGCGTTCATGGTGCATCTGTTCCTGTTTTCTTTATCTTCTTTGGAAAAATTATTAACGTCGTAGGTTTGGCTTACCTTTTTCCTAAAGAAGCTTCCCACCAAGTTGCCAAGGTTGGGAACTTTATGCACTTTATAAGCCGATTTATAGCGGGCTTTATCATCGGTTTTTTAAGAGTTTGGCAGATCAGTTTAGTGACACTTGCTATTGTTCCTTTGATTGCTATTGCTGGAGGTCTTTATGCATATGTGACTTTTGGCCTTATTGCAAAAGTTCGTAAATCATATCTGAGGGCGGGTGAAATTGCTCAGGAGGTGATAGGAAATGTGAGAACTGTGCAAGCATTTGGAGGAGAACAAAGAGCAGTGATATCATACAAAGTGGCTCTGAGGAACACATATAAGAATGGTCGAAAAGCCGGTTTAGCAAAGGGTTTAGGACTAGGCTCTATGCATTGTGTTCTTTTTTTATCATGGGCTCTGCTTGTTTGGTTCACCAGCATTATTGTTCACAAGAACATCGCCAATGGTGGAAAGGCTTTCGCAACCATGCTCAATGTGGCAGCACCAGACATTTCTGCATTTATCAGAGCAAAGACAGCAGCTTATCCCATTTTTGAGATGATAGAGAGGGACATAGTTAACAAAAATAGCTCAAAAGATGATCGAAACATAAAGAACTCAGCAGAAATATCCCAAACAGCAACTATTGGTGGTAGTTTTCATTCAGATAGAAGCTCTATTGGTCATGCACTTGCTGATGAACCAAGAAGTGTTGAAAAGCCAAGGCATGTTTCATTACTAAGATTATATTCAATGATTGTTTTTGGAACCTTAGCTGCTTTTACTACTGGTGCTCTTATGCCGCTTTTTGCTCTTGGAATATCGCATGCTCTTGTATCATATTATATGGATTGGGATAGCACACGCCACGAAGTGAAAAAAATTGCATTCCTCTTCTGTGGAGCGGCAATTGTAGCTATCACTGCTTATTCCATTGAACATCTTTCTTTTGGAATCATGGGAGAACGACTTACCCTTCGTGTCAGAGGAATTATGCTTTCTGCTATTTTGAAGAATGAAATCGGTTGGTTTGATGATACAAGAAACACAAGTTCTATGCTTTCATCACGTTTAGAGACTGATGCAACTTTGTTAAAAACTATAGTTGTGGACCGTTCTACAATTCTCTTGCAGAATGTTGGTCTAGTTGTTACTGCACTTGTTATTGCATTCATTTTGAACTGGAGGATAACACTTGTTGTCTTGGCCACATATCCCTTGATAATAAGTGGTCACATTGACAGAAAATCAGGAATAACACATGATGTTGGAGAAGAGTTAATGACAGTGGAAGGAACAATTGAGCTAAAAAGGATCAATTTTGTCTATCCTTCAAGACCAAACGTCGTTATTTTTAACAATTTCAATCTAATAGTGCCTTCAGGGAAGAGTCTTGCCTTGGTAGGGCACAGTGGTTCTGGTAAAAGCTCAATAATCTCCCTTATACTCAGATTTTATGATCCAACATCTGGGAAGGTGATGATTGAT |
| Protein sequence |
>Msa1291880 MPRIIPDVAYRSFPPYIAGPNISAFIRTKAAAYHIFEMINQDIVGKNSSKTELQGRKVPLLKLFTFADFYDYVLMVVGSVGACVHGASVPVFFIFFGKIINVVGLAYLFPKEASHQVAKVGNFMHFISRFIAGFIIGFLRVWQISLVTLAIVPLIAIAGGLYAYVTFGLIAKVRKSYLRAGEIAQEVIGNVRTVQAFGGEQRAVISYKVALRNTYKNGRKAGLAKGLGLGSMHCVLFLSWALLVWFTSIIVHKNIANGGKAFATMLNVAAPDISAFIRAKTAAYPIFEMIERDIVNKNSSKDDRNIKNSAEISQTATIGGSFHSDRSSIGHALADEPRSVEKPRHVSLLRLYSMIVFGTLAAFTTGALMPLFALGISHALVSYYMDWDSTRHEVKKIAFLFCGAAIVAITAYSIEHLSFGIMGERLTLRVRGIMLSAILKNEIGWFDDTRNTSSMLSSRLETDATLLKTIVVDRSTILLQNVGLVVTALVIAFILNWRITLVVLATYPLIISGHIDRKSGITHDVGEELMTVEGTIELKRINFVYPSRPNVVIFNNFNLIVPSGKSLALVGHSGSGKSSIISLILRFYDPTSGKVMID |
