Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1307770 | A0A072TNP5 | 53.441 | 247 | 92 | 6 | 14 | 241 | 6 | 248 | 7.16e-72 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0021980 | Msa1307770 | 0.818305 | 2.101301e-52 | -8.615850e-47 |
| Msa0610850 | Msa1307770 | 0.823146 | 1.620201e-53 | -8.615850e-47 |
| Msa0641020 | Msa1307770 | 0.813241 | 2.828132e-51 | -8.615850e-47 |
| Msa0641030 | Msa1307770 | 0.831494 | 1.616701e-55 | -8.615850e-47 |
| Msa0651340 | Msa1307770 | 0.807926 | 3.981728e-50 | -8.615850e-47 |
| Msa0657860 | Msa1307770 | 0.840085 | 1.075165e-57 | -8.615850e-47 |
| Msa0679560 | Msa1307770 | 0.848555 | 5.689489e-60 | -8.615850e-47 |
| Msa0739410 | Msa1307770 | 0.827414 | 1.585354e-54 | -8.615850e-47 |
| Msa0759380 | Msa1307770 | 0.806565 | 7.732436e-50 | -8.615850e-47 |
| Msa0141680 | Msa1307770 | 0.820838 | 5.550871e-53 | -8.615850e-47 |
| Msa0212490 | Msa1307770 | -0.820544 | 6.487039e-53 | -8.615850e-47 |
| Msa1232330 | Msa1307770 | 0.802441 | 5.600842e-49 | -8.615850e-47 |
| Msa1233740 | Msa1307770 | 0.878566 | 2.531723e-69 | -8.615850e-47 |
| Msa1275340 | Msa1307770 | 0.800460 | 1.426356e-48 | -8.615850e-47 |
| Msa1307770 | Msa1413880 | 0.800821 | 1.204088e-48 | -8.615850e-47 |
| Msa1307770 | Msa1445250 | 0.835523 | 1.597190e-56 | -8.615850e-47 |
| Msa1307770 | Msa1457070 | 0.815815 | 7.621035e-52 | -8.615850e-47 |
| Msa0365360 | Msa1307770 | 0.827565 | 1.458595e-54 | -8.615850e-47 |
| Msa0389050 | Msa1307770 | 0.803960 | 2.715806e-49 | -8.615850e-47 |
| Msa0445830 | Msa1307770 | 0.811991 | 5.306705e-51 | -8.615850e-47 |
| Msa0457370 | Msa1307770 | 0.828746 | 7.576436e-55 | -8.615850e-47 |
| Msa1009170 | Msa1307770 | 0.817050 | 4.031875e-52 | -8.615850e-47 |
| Msa1055710 | Msa1307770 | 0.837310 | 5.603757e-57 | -8.615850e-47 |
| Msa0252880 | Msa1307770 | -0.826220 | 3.057521e-54 | -8.615850e-47 |
| Msa0329650 | Msa1307770 | -0.818137 | 2.292700e-52 | -8.615850e-47 |
| Msa1138490 | Msa1307770 | 0.808457 | 3.068095e-50 | -8.615850e-47 |
| Msa1145930 | Msa1307770 | 0.806847 | 6.743666e-50 | -8.615850e-47 |
| Msa1190460 | Msa1307770 | 0.804614 | 1.985573e-49 | -8.615850e-47 |
| Msa1211280 | Msa1307770 | 0.804315 | 2.292025e-49 | -8.615850e-47 |
| Msa0535020 | Msa1307770 | 0.822561 | 2.217918e-53 | -8.615850e-47 |
| Msa0535050 | Msa1307770 | 0.825046 | 5.801886e-54 | -8.615850e-47 |
| Msa0544930 | Msa1307770 | 0.836159 | 1.101590e-56 | -8.615850e-47 |
| Msa0566010 | Msa1307770 | 0.814591 | 1.425073e-51 | -8.615850e-47 |
| Msa0575540 | Msa1307770 | 0.830758 | 2.451846e-55 | -8.615850e-47 |
| Msa0578890 | Msa1307770 | 0.813055 | 3.105881e-51 | -8.615850e-47 |
| Msa0930090 | Msa1307770 | 0.823790 | 1.146025e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1307770 | MtrunA17_Chr8g0343361 | 53.441 | 247 | 92 | 6 | 14 | 241 | 6 | 248 | 1.38e-75 | 229 |
| Msa1307770 | MtrunA17_Chr8g0343261 | 50.202 | 247 | 98 | 5 | 13 | 241 | 6 | 245 | 6.54e-74 | 224 |
| Msa1307770 | MtrunA17_Chr8g0343191 | 55.263 | 190 | 66 | 3 | 56 | 227 | 1 | 189 | 1.38e-63 | 197 |
| Msa1307770 | MtrunA17_Chr8g0343301 | 45.614 | 228 | 112 | 5 | 17 | 238 | 9 | 230 | 1.82e-57 | 182 |
| Msa1307770 | MtrunA17_Chr8g0343311 | 46.531 | 245 | 111 | 8 | 12 | 241 | 4 | 243 | 2.09e-57 | 183 |
| Msa1307770 | MtrunA17_Chr8g0343221 | 46.948 | 213 | 105 | 4 | 28 | 237 | 21 | 228 | 7.66e-57 | 181 |
| Msa1307770 | MtrunA17_Chr8g0343351 | 53.371 | 178 | 72 | 2 | 56 | 227 | 1 | 173 | 1.52e-56 | 178 |
| Msa1307770 | MtrunA17_Chr8g0343211 | 54.430 | 158 | 53 | 3 | 56 | 195 | 1 | 157 | 8.68e-49 | 158 |
| Msa1307770 | MtrunA17_Chr8g0343231 | 47.953 | 171 | 79 | 5 | 56 | 220 | 1 | 167 | 2.92e-45 | 149 |
| Msa1307770 | MtrunA17_Chr7g0235131 | 42.236 | 161 | 89 | 3 | 45 | 203 | 7 | 165 | 2.27e-34 | 121 |
| Msa1307770 | MtrunA17_Chr1g0182721 | 34.529 | 223 | 124 | 6 | 3 | 217 | 30 | 238 | 3.45e-31 | 115 |
| Msa1307770 | MtrunA17_Chr7g0254671 | 32.821 | 195 | 110 | 6 | 40 | 223 | 15 | 199 | 5.16e-23 | 92.8 |
| Msa1307770 | MtrunA17_Chr7g0262731 | 35.802 | 162 | 96 | 2 | 57 | 217 | 434 | 588 | 2.00e-22 | 95.5 |
| Msa1307770 | MtrunA17_Chr8g0343241 | 55.405 | 74 | 33 | 0 | 110 | 183 | 4 | 77 | 1.39e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1307770 | AT3G13950.1 | 29.756 | 205 | 114 | 8 | 17 | 215 | 5 | 185 | 6.60e-18 | 79.7 |
| Msa1307770 | AT4G13266.1 | 26.630 | 184 | 114 | 5 | 47 | 221 | 26 | 197 | 1.83e-14 | 70.5 |
Find 52 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAATAAGGTTAGTTATT+TGG | 0.107289 | 8_4:-3694596 | Msa1307770:CDS |
| CACAATAAGGTTAGTTATTT+GGG | 0.139676 | 8_4:-3694595 | Msa1307770:CDS |
| AACAAATTGTGTTTGAAATA+AGG | 0.219790 | 8_4:+3694853 | None:intergenic |
| ATTGGATTGTTATCAATTCT+TGG | 0.282494 | 8_4:-3694627 | Msa1307770:CDS |
| GCTTCTCCGGGACATAGTTT+TGG | 0.283427 | 8_4:+3694915 | None:intergenic |
| AGGAACCTATGATAATCATT+AGG | 0.312240 | 8_4:+3694873 | None:intergenic |
| AAAGAGTACAAAGGAGATTT+CGG | 0.335902 | 8_4:-3694526 | Msa1307770:CDS |
| AAGTACATTGTAGCAAAATC+AGG | 0.342618 | 8_4:-3695128 | Msa1307770:CDS |
| GTGGAATTCGTTTGCATCAT+AGG | 0.363401 | 8_4:-3694794 | Msa1307770:CDS |
| CCTTTCTTTGAACTTGTTGT+CGG | 0.365741 | 8_4:+3695158 | None:intergenic |
| TTGGTTTGTGCCTCACAATA+AGG | 0.374489 | 8_4:-3694608 | Msa1307770:CDS |
| ATGTCCCGGAGAAGCTGTTT+TGG | 0.376789 | 8_4:-3694907 | Msa1307770:CDS |
| ATAACCCAAATGGATAATTC+TGG | 0.383482 | 8_4:-3695233 | None:intergenic |
| TATACCCTAATGATTATCAT+AGG | 0.385433 | 8_4:-3694878 | Msa1307770:CDS |
| TCATCGCCGTTGTCTTTGTC+TGG | 0.386438 | 8_4:+3694939 | None:intergenic |
| TAACAAGTTTCTTGAGAAGT+TGG | 0.386760 | 8_4:-3694661 | Msa1307770:CDS |
| CCTATGTGGATGTTAGCAAT+AGG | 0.389609 | 8_4:-3694768 | Msa1307770:CDS |
| AATAGCCAAAACAGCTTCTC+CGG | 0.412499 | 8_4:+3694902 | None:intergenic |
| GGAACCTATGATAATCATTA+GGG | 0.412647 | 8_4:+3694874 | None:intergenic |
| AACTTCTTCTTCATGTGCTA+TGG | 0.417595 | 8_4:+3695199 | None:intergenic |
| TAGCAAAATCAGGGCACCTT+TGG | 0.421626 | 8_4:-3695118 | Msa1307770:CDS |
| GTTAGACCAGTAAAAGAAAC+AGG | 0.427303 | 8_4:-3694981 | Msa1307770:CDS |
| TGACTGTTGCTATTAAACTT+AGG | 0.443404 | 8_4:+3695048 | None:intergenic |
| CTCAACAATGACATTTCAAA+TGG | 0.459232 | 8_4:-3695024 | Msa1307770:CDS |
| TTTCCTCAACAGAAGTAAGA+TGG | 0.459688 | 8_4:-3694562 | Msa1307770:CDS |
| TGTCTTCTTCATTCATCCAA+AGG | 0.467265 | 8_4:+3695102 | None:intergenic |
| AAGGTTAGTTATTTGGGGAG+TGG | 0.468986 | 8_4:-3694589 | Msa1307770:CDS |
| ACATGAAGAAGAAGTTGGTC+AGG | 0.478107 | 8_4:-3695192 | Msa1307770:CDS |
| CAAATGGCGTTTAATCCACC+AGG | 0.491341 | 8_4:-3695008 | Msa1307770:CDS |
| CAACAAGTTCAAAGAAAGGT+TGG | 0.494705 | 8_4:-3695154 | Msa1307770:CDS |
| CTATGTGGATGTTAGCAATA+GGG | 0.500543 | 8_4:-3694767 | Msa1307770:CDS |
| TCATGTGCTATGGATTGTGT+TGG | 0.508903 | 8_4:+3695209 | None:intergenic |
| ATGAAGAAGACAAGAAAGAA+TGG | 0.521764 | 8_4:-3695091 | Msa1307770:CDS |
| TTTACTGGTCTAACACCACC+TGG | 0.544912 | 8_4:+3694990 | None:intergenic |
| ATTTGTCTCTTGCTTGTGAG+TGG | 0.549588 | 8_4:-3694813 | Msa1307770:CDS |
| TAGCCATCTTACTTCTGTTG+AGG | 0.559009 | 8_4:+3694559 | Msa1307760:CDS |
| ATAGCACATGAAGAAGAAGT+TGG | 0.565206 | 8_4:-3695197 | Msa1307770:CDS |
| TTGTCTTTGTCTGGAAGACA+TGG | 0.568346 | 8_4:+3694948 | None:intergenic |
| ACAATAAGGTTAGTTATTTG+GGG | 0.580656 | 8_4:-3694594 | Msa1307770:CDS |
| TGCATCATAGGTTACCTATG+TGG | 0.581559 | 8_4:-3694782 | Msa1307770:CDS |
| CCGACAACAAGTTCAAAGAA+AGG | 0.582659 | 8_4:-3695158 | Msa1307770:CDS |
| ATAGCCAAAACAGCTTCTCC+GGG | 0.585715 | 8_4:+3694903 | None:intergenic |
| GAATGGTTAAACTACATGAG+AGG | 0.596068 | 8_4:-3695074 | Msa1307770:CDS |
| AGTACATTGTAGCAAAATCA+GGG | 0.596650 | 8_4:-3695127 | Msa1307770:CDS |
| AATGGTTAAACTACATGAGA+GGG | 0.596824 | 8_4:-3695073 | Msa1307770:CDS |
| CCTATTGCTAACATCCACAT+AGG | 0.600445 | 8_4:+3694768 | None:intergenic |
| GATGACCCAAAACTATGTCC+CGG | 0.622580 | 8_4:-3694921 | Msa1307770:CDS |
| AAATAACTAACCTTATTGTG+AGG | 0.630050 | 8_4:+3694598 | Msa1307760:CDS |
| ACTGGTCTAACACCACCTGG+TGG | 0.656114 | 8_4:+3694993 | None:intergenic |
| GACTGAACAAAAGAGTACAA+AGG | 0.662802 | 8_4:-3694535 | Msa1307770:CDS |
| TGTCTTCCAGACAAAGACAA+CGG | 0.729308 | 8_4:-3694945 | Msa1307770:CDS |
| ATGGCGTTTAATCCACCAGG+TGG | 0.730048 | 8_4:-3695005 | Msa1307770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAAATTGTGTTTGAAATA+AGG | + | chr8_4:3694895-3694914 | None:intergenic | 20.0% |
| ! | TATACCCTAATGATTATCAT+AGG | - | chr8_4:3694867-3694886 | Msa1307770:CDS | 25.0% |
| !!! | TTGTTAGCTTTTTCAAAAAC+AGG | + | chr8_4:3695070-3695089 | None:intergenic | 25.0% |
| ! | ATTGGATTGTTATCAATTCT+TGG | - | chr8_4:3695118-3695137 | Msa1307770:CDS | 25.0% |
| ! | AAATAACTAACCTTATTGTG+AGG | + | chr8_4:3695150-3695169 | None:intergenic | 25.0% |
| !! | TCACAATAAGGTTAGTTATT+TGG | - | chr8_4:3695149-3695168 | Msa1307770:CDS | 25.0% |
| !! | CACAATAAGGTTAGTTATTT+GGG | - | chr8_4:3695150-3695169 | Msa1307770:CDS | 25.0% |
| !! | ACAATAAGGTTAGTTATTTG+GGG | - | chr8_4:3695151-3695170 | Msa1307770:CDS | 25.0% |
| AAGTACATTGTAGCAAAATC+AGG | - | chr8_4:3694617-3694636 | Msa1307770:CDS | 30.0% | |
| AGTACATTGTAGCAAAATCA+GGG | - | chr8_4:3694618-3694637 | Msa1307770:CDS | 30.0% | |
| ATGAAGAAGACAAGAAAGAA+TGG | - | chr8_4:3694654-3694673 | Msa1307770:CDS | 30.0% | |
| AATGGTTAAACTACATGAGA+GGG | - | chr8_4:3694672-3694691 | Msa1307770:CDS | 30.0% | |
| ! | TGACTGTTGCTATTAAACTT+AGG | + | chr8_4:3694700-3694719 | Msa1307760:CDS | 30.0% |
| CTCAACAATGACATTTCAAA+TGG | - | chr8_4:3694721-3694740 | Msa1307770:CDS | 30.0% | |
| !! | TCATTTCCTGTTTCTTTTAC+TGG | + | chr8_4:3694773-3694792 | None:intergenic | 30.0% |
| GGAACCTATGATAATCATTA+GGG | + | chr8_4:3694874-3694893 | None:intergenic | 30.0% | |
| AGGAACCTATGATAATCATT+AGG | + | chr8_4:3694875-3694894 | None:intergenic | 30.0% | |
| TAACAAGTTTCTTGAGAAGT+TGG | - | chr8_4:3695084-3695103 | Msa1307770:CDS | 30.0% | |
| !!! | TTGAGAAGTTGGTTTTTACT+TGG | - | chr8_4:3695095-3695114 | Msa1307770:CDS | 30.0% |
| !!! | AAGTTGGTTTTTACTTGGAT+TGG | - | chr8_4:3695100-3695119 | Msa1307770:CDS | 30.0% |
| ! | AAAGAGTACAAAGGAGATTT+CGG | - | chr8_4:3695219-3695238 | Msa1307770:CDS | 30.0% |
| AACTTCTTCTTCATGTGCTA+TGG | + | chr8_4:3694549-3694568 | Msa1307760:intron | 35.0% | |
| ATAGCACATGAAGAAGAAGT+TGG | - | chr8_4:3694548-3694567 | Msa1307770:CDS | 35.0% | |
| !! | CCTTTCTTTGAACTTGTTGT+CGG | + | chr8_4:3694590-3694609 | Msa1307760:CDS | 35.0% |
| CAACAAGTTCAAAGAAAGGT+TGG | - | chr8_4:3694591-3694610 | Msa1307770:CDS | 35.0% | |
| TGTCTTCTTCATTCATCCAA+AGG | + | chr8_4:3694646-3694665 | Msa1307760:CDS | 35.0% | |
| GAATGGTTAAACTACATGAG+AGG | - | chr8_4:3694671-3694690 | Msa1307770:CDS | 35.0% | |
| GTTAGACCAGTAAAAGAAAC+AGG | - | chr8_4:3694764-3694783 | Msa1307770:CDS | 35.0% | |
| CTATGTGGATGTTAGCAATA+GGG | - | chr8_4:3694978-3694997 | Msa1307770:CDS | 35.0% | |
| !!! | GCTTTTTCAAAAACAGGATC+TGG | + | chr8_4:3695064-3695083 | None:intergenic | 35.0% |
| TTTCCTCAACAGAAGTAAGA+TGG | - | chr8_4:3695183-3695202 | Msa1307770:CDS | 35.0% | |
| ! | GACTGAACAAAAGAGTACAA+AGG | - | chr8_4:3695210-3695229 | Msa1307770:CDS | 35.0% |
| ! | TCATGTGCTATGGATTGTGT+TGG | + | chr8_4:3694539-3694558 | Msa1307760:intron | 40.0% |
| ! | ACATGAAGAAGAAGTTGGTC+AGG | - | chr8_4:3694553-3694572 | Msa1307770:CDS | 40.0% |
| CCGACAACAAGTTCAAAGAA+AGG | - | chr8_4:3694587-3694606 | Msa1307770:CDS | 40.0% | |
| TTGTCTTTGTCTGGAAGACA+TGG | + | chr8_4:3694800-3694819 | None:intergenic | 40.0% | |
| TGTCTTCCAGACAAAGACAA+CGG | - | chr8_4:3694800-3694819 | Msa1307770:CDS | 40.0% | |
| AATAGCCAAAACAGCTTCTC+CGG | + | chr8_4:3694846-3694865 | None:intergenic | 40.0% | |
| ATTTGTCTCTTGCTTGTGAG+TGG | - | chr8_4:3694932-3694951 | Msa1307770:CDS | 40.0% | |
| GTGGAATTCGTTTGCATCAT+AGG | - | chr8_4:3694951-3694970 | Msa1307770:CDS | 40.0% | |
| TGCATCATAGGTTACCTATG+TGG | - | chr8_4:3694963-3694982 | Msa1307770:CDS | 40.0% | |
| CCTATTGCTAACATCCACAT+AGG | + | chr8_4:3694980-3694999 | None:intergenic | 40.0% | |
| CCTATGTGGATGTTAGCAAT+AGG | - | chr8_4:3694977-3694996 | Msa1307770:CDS | 40.0% | |
| !! | TTGGTTTGTGCCTCACAATA+AGG | - | chr8_4:3695137-3695156 | Msa1307770:CDS | 40.0% |
| ! | AAGGTTAGTTATTTGGGGAG+TGG | - | chr8_4:3695156-3695175 | Msa1307770:CDS | 40.0% |
| TAGCCATCTTACTTCTGTTG+AGG | + | chr8_4:3695189-3695208 | None:intergenic | 40.0% | |
| ! | TAGCAAAATCAGGGCACCTT+TGG | - | chr8_4:3694627-3694646 | Msa1307770:CDS | 45.0% |
| CAAATGGCGTTTAATCCACC+AGG | - | chr8_4:3694737-3694756 | Msa1307770:CDS | 45.0% | |
| ! | TTTACTGGTCTAACACCACC+TGG | + | chr8_4:3694758-3694777 | None:intergenic | 45.0% |
| GATGACCCAAAACTATGTCC+CGG | - | chr8_4:3694824-3694843 | Msa1307770:CDS | 45.0% | |
| ! | CTTCTCCGGGACATAGTTTT+GGG | + | chr8_4:3694832-3694851 | None:intergenic | 45.0% |
| ATAGCCAAAACAGCTTCTCC+GGG | + | chr8_4:3694845-3694864 | None:intergenic | 45.0% | |
| ATGGCGTTTAATCCACCAGG+TGG | - | chr8_4:3694740-3694759 | Msa1307770:CDS | 50.0% | |
| TCATCGCCGTTGTCTTTGTC+TGG | + | chr8_4:3694809-3694828 | None:intergenic | 50.0% | |
| ! | GCTTCTCCGGGACATAGTTT+TGG | + | chr8_4:3694833-3694852 | None:intergenic | 50.0% |
| ! | ATGTCCCGGAGAAGCTGTTT+TGG | - | chr8_4:3694838-3694857 | Msa1307770:CDS | 50.0% |
| ! | ACTGGTCTAACACCACCTGG+TGG | + | chr8_4:3694755-3694774 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 3694521 | 3695246 | 3694521 | ID=Msa1307770;Name=Msa1307770 |
| chr8_4 | mRNA | 3694521 | 3695246 | 3694521 | ID=Msa1307770-mRNA-1;Parent=Msa1307770;Name=Msa1307770-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|241 |
| chr8_4 | exon | 3694521 | 3695246 | 3694521 | ID=Msa1307770-mRNA-1:exon:1111;Parent=Msa1307770-mRNA-1 |
| chr8_4 | CDS | 3694521 | 3695246 | 3694521 | ID=Msa1307770-mRNA-1:cds;Parent=Msa1307770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
