AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1343320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGCAACCTTATCATTTC+TGG 0.089860 tig0006360:+2664 Msa1343310:three_prime_UTR
CCAAAGCATATGCACTCTTT+TGG 0.266007 tig0006360:-3832 Msa1343320:CDS
GTTATCATTCTCATGTTCTA+TGG 0.266198 tig0006360:+3893 None:intergenic
CCAAAAGAGTGCATATGCTT+TGG 0.353547 tig0006360:+3832 None:intergenic
TGTTATCAAGGAAGATCTTT+GGG 0.357140 tig0006360:+3862 None:intergenic
TTGTTATCAAGGAAGATCTT+TGG 0.360041 tig0006360:+3861 None:intergenic
TGACCTCTTAGCAAGAAATA+TGG 0.407153 tig0006360:-3919 Msa1343320:CDS
TTTGGAAAACATTGTTATCA+AGG 0.418543 tig0006360:+3850 None:intergenic
TTATCATTCTCATGTTCTAT+GGG 0.443329 tig0006360:+3894 None:intergenic
ATGTGCTAAACGATGATGTA+TGG 0.472847 tig0006360:+2633 Msa1343310:three_prime_UTR
AACGATGATGTATGGGCGGC+AGG 0.475444 tig0006360:+2641 Msa1343310:three_prime_UTR
AGATGCATGGTTAAGTTCCT+AGG 0.485308 tig0006360:-3807 Msa1343320:intron
GAGCCATATTTCTTGCTAAG+AGG 0.549970 tig0006360:+3916 None:intergenic
CAATGAATGAGGATTTGAAC+AGG 0.554172 tig0006360:+2556 Msa1343310:intron
TTAGAATATGACAATGAATG+AGG 0.563336 tig0006360:+2545 Msa1343310:intron
TCGTTTAGCACATCCTCTTG+TGG 0.566941 tig0006360:-2623 Msa1343320:CDS
TGAATGAGGATTTGAACAGG+TGG 0.604345 tig0006360:+2559 Msa1343310:intron
ATGCTGTTTGTGCGATGTCG+CGG 0.620832 tig0006360:+2584 Msa1343310:three_prime_UTR
CATAAGTCACTCTCCACAAG+AGG 0.625552 tig0006360:+2610 Msa1343310:three_prime_UTR
TGTGCTAAACGATGATGTAT+GGG 0.641420 tig0006360:+2634 Msa1343310:three_prime_UTR
GAATGAGGATTTGAACAGGT+GGG 0.644540 tig0006360:+2560 Msa1343310:intron
TATCAAGGAAGATCTTTGGG+AGG 0.645228 tig0006360:+3865 None:intergenic
CTGTTTGTGCGATGTCGCGG+AGG 0.676245 tig0006360:+2587 Msa1343310:three_prime_UTR
GCTAAACGATGATGTATGGG+CGG 0.742622 tig0006360:+2637 Msa1343310:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTAATTGTTACAAAAT+TGG - tig0006360:3630-3649 Msa1343320:intron 10.0%
!! AAATTTAATTGTTACAAAAT+TGG - tig0006360:3630-3649 Msa1343320:intron 10.0%
!!! AACATGAAGTTTTTTTTTAT+GGG + tig0006360:3405-3424 None:intergenic 15.0%
!!! AACATGAAGTTTTTTTTTAT+GGG + tig0006360:3405-3424 None:intergenic 15.0%
!! CATAAAGATTTCATTAATCT+TGG - tig0006360:2878-2897 Msa1343320:intron 20.0%
!!! TTTACATTTTTAGAAGTCTA+AGG - tig0006360:2937-2956 Msa1343320:intron 20.0%
!!! CAACATGAAGTTTTTTTTTA+TGG + tig0006360:3406-3425 None:intergenic 20.0%
!! TTAATTGTTACAAAATTGGT+TGG - tig0006360:3634-3653 Msa1343320:intron 20.0%
!! TATTTGTAGCATGTAATTAT+AGG + tig0006360:3760-3779 None:intergenic 20.0%
!! CATAAAGATTTCATTAATCT+TGG - tig0006360:2878-2897 Msa1343320:intron 20.0%
!!! TTTACATTTTTAGAAGTCTA+AGG - tig0006360:2937-2956 Msa1343320:intron 20.0%
!!! CAACATGAAGTTTTTTTTTA+TGG + tig0006360:3406-3425 None:intergenic 20.0%
!! TTAATTGTTACAAAATTGGT+TGG - tig0006360:3634-3653 Msa1343320:intron 20.0%
!! TATTTGTAGCATGTAATTAT+AGG + tig0006360:3760-3779 None:intergenic 20.0%
! TTATCATTCTCATGTTCTAT+GGG + tig0006360:2596-2615 Msa1343310:three_prime_UTR 25.0%
! TTTGGAAAACATTGTTATCA+AGG + tig0006360:2640-2659 Msa1343310:three_prime_UTR 25.0%
!!! ATCATAAGCTTTTTTTACCT+AGG + tig0006360:2700-2719 Msa1343310:three_prime_UTR 25.0%
! AAGCTTATGATACAATACTA+TGG - tig0006360:2709-2728 Msa1343320:intron 25.0%
! AACAAAAAACAAAAGTCGAT+TGG - tig0006360:2826-2845 Msa1343320:intron 25.0%
! GATGAATATTTGATGCATAT+GGG + tig0006360:3014-3033 Msa1343310:three_prime_UTR 25.0%
! AGATGAATATTTGATGCATA+TGG + tig0006360:3015-3034 Msa1343310:three_prime_UTR 25.0%
!! TACATTGATTACCTTATCTA+AGG + tig0006360:3174-3193 None:intergenic 25.0%
!! ATAAACTGGTCTAATAGTAA+GGG + tig0006360:3258-3277 None:intergenic 25.0%
!!! GTTGTTTTCATTTTAGACTA+AGG + tig0006360:3311-3330 None:intergenic 25.0%
! AAATGTAAGCATGAATAAAC+TGG - tig0006360:3512-3531 Msa1343320:intron 25.0%
! GTACTTGATAAATTGAAGAA+AGG + tig0006360:3547-3566 None:intergenic 25.0%
! TTTGATCATATGTATGATCA+TGG - tig0006360:3576-3595 Msa1343320:intron 25.0%
!! TAGATTTTCCAGAAATGATA+AGG - tig0006360:3815-3834 Msa1343320:CDS 25.0%
! TTAGAATATGACAATGAATG+AGG + tig0006360:3945-3964 None:intergenic 25.0%
! TTATCATTCTCATGTTCTAT+GGG + tig0006360:2596-2615 Msa1343310:three_prime_UTR 25.0%
! TTTGGAAAACATTGTTATCA+AGG + tig0006360:2640-2659 Msa1343310:three_prime_UTR 25.0%
!!! ATCATAAGCTTTTTTTACCT+AGG + tig0006360:2700-2719 Msa1343310:three_prime_UTR 25.0%
! AAGCTTATGATACAATACTA+TGG - tig0006360:2709-2728 Msa1343320:intron 25.0%
! AACAAAAAACAAAAGTCGAT+TGG - tig0006360:2826-2845 Msa1343320:intron 25.0%
! GATGAATATTTGATGCATAT+GGG + tig0006360:3014-3033 Msa1343310:three_prime_UTR 25.0%
! AGATGAATATTTGATGCATA+TGG + tig0006360:3015-3034 Msa1343310:three_prime_UTR 25.0%
!! TACATTGATTACCTTATCTA+AGG + tig0006360:3174-3193 None:intergenic 25.0%
!! ATAAACTGGTCTAATAGTAA+GGG + tig0006360:3258-3277 None:intergenic 25.0%
!!! GTTGTTTTCATTTTAGACTA+AGG + tig0006360:3311-3330 None:intergenic 25.0%
! AAATGTAAGCATGAATAAAC+TGG - tig0006360:3512-3531 Msa1343320:intron 25.0%
! GTACTTGATAAATTGAAGAA+AGG + tig0006360:3547-3566 None:intergenic 25.0%
! TTTGATCATATGTATGATCA+TGG - tig0006360:3576-3595 Msa1343320:intron 25.0%
!! TAGATTTTCCAGAAATGATA+AGG - tig0006360:3815-3834 Msa1343320:CDS 25.0%
! TTAGAATATGACAATGAATG+AGG + tig0006360:3945-3964 None:intergenic 25.0%
GTTATCATTCTCATGTTCTA+TGG + tig0006360:2597-2616 Msa1343310:three_prime_UTR 30.0%
TGTTATCAAGGAAGATCTTT+GGG + tig0006360:2628-2647 Msa1343310:three_prime_UTR 30.0%
TTGTTATCAAGGAAGATCTT+TGG + tig0006360:2629-2648 Msa1343310:three_prime_UTR 30.0%
CTTATGATACAATACTATGG+AGG - tig0006360:2712-2731 Msa1343320:intron 30.0%
TATGGAGGAGAAATTGAAAA+AGG - tig0006360:2727-2746 Msa1343320:intron 30.0%
ATAAAAGGGGCTTACATATA+GGG + tig0006360:3057-3076 Msa1343310:three_prime_UTR 30.0%
AATAAAAGGGGCTTACATAT+AGG + tig0006360:3058-3077 Msa1343310:three_prime_UTR 30.0%
! ACCTTTTGCCTCTAATAAAA+GGG + tig0006360:3071-3090 Msa1343310:three_prime_UTR 30.0%
GATTTAGTGTGAATTTGCAA+CGG - tig0006360:3128-3147 Msa1343320:intron 30.0%
! CATAAACTGGTCTAATAGTA+AGG + tig0006360:3259-3278 None:intergenic 30.0%
ACTATTAGACCAGTTTATGT+TGG - tig0006360:3260-3279 Msa1343320:intron 30.0%
GTATATGTGAACATTGTAAG+TGG + tig0006360:3374-3393 None:intergenic 30.0%
CAAATTATTCTCCTATCAAG+AGG + tig0006360:3663-3682 None:intergenic 30.0%
AGGAGAATAATTTGAGAACA+CGG - tig0006360:3669-3688 Msa1343320:intron 30.0%
TTTAGCAACCTTATCATTTC+TGG + tig0006360:3826-3845 None:intergenic 30.0%
GTTATCATTCTCATGTTCTA+TGG + tig0006360:2597-2616 Msa1343310:three_prime_UTR 30.0%
TGTTATCAAGGAAGATCTTT+GGG + tig0006360:2628-2647 Msa1343310:three_prime_UTR 30.0%
TTGTTATCAAGGAAGATCTT+TGG + tig0006360:2629-2648 Msa1343310:three_prime_UTR 30.0%
CTTATGATACAATACTATGG+AGG - tig0006360:2712-2731 Msa1343320:intron 30.0%
TATGGAGGAGAAATTGAAAA+AGG - tig0006360:2727-2746 Msa1343320:intron 30.0%
ATAAAAGGGGCTTACATATA+GGG + tig0006360:3057-3076 Msa1343310:three_prime_UTR 30.0%
AATAAAAGGGGCTTACATAT+AGG + tig0006360:3058-3077 Msa1343310:three_prime_UTR 30.0%
! ACCTTTTGCCTCTAATAAAA+GGG + tig0006360:3071-3090 Msa1343310:three_prime_UTR 30.0%
GATTTAGTGTGAATTTGCAA+CGG - tig0006360:3128-3147 Msa1343320:intron 30.0%
! CATAAACTGGTCTAATAGTA+AGG + tig0006360:3259-3278 None:intergenic 30.0%
ACTATTAGACCAGTTTATGT+TGG - tig0006360:3260-3279 Msa1343320:intron 30.0%
GTATATGTGAACATTGTAAG+TGG + tig0006360:3374-3393 None:intergenic 30.0%
CAAATTATTCTCCTATCAAG+AGG + tig0006360:3663-3682 None:intergenic 30.0%
AGGAGAATAATTTGAGAACA+CGG - tig0006360:3669-3688 Msa1343320:intron 30.0%
TTTAGCAACCTTATCATTTC+TGG + tig0006360:3826-3845 None:intergenic 30.0%
TGACCTCTTAGCAAGAAATA+TGG - tig0006360:2568-2587 Msa1343320:CDS 35.0%
AGGAGAAATTGAAAAAGGTG+TGG - tig0006360:2732-2751 Msa1343320:intron 35.0%
! TCATGATCTTGAAACCGTTT+TGG + tig0006360:2763-2782 Msa1343310:three_prime_UTR 35.0%
GTTTAACCTTTGTTGAGACA+CGG - tig0006360:2848-2867 Msa1343320:intron 35.0%
AATTAACCGTGTCTCAACAA+AGG + tig0006360:2857-2876 Msa1343310:three_prime_UTR 35.0%
GAATATTTGATGCATATGGG+TGG + tig0006360:3011-3030 Msa1343310:three_prime_UTR 35.0%
! ATGTAAGCCCCTTTTATTAG+AGG - tig0006360:3060-3079 Msa1343320:intron 35.0%
! CCTTTTGCCTCTAATAAAAG+GGG + tig0006360:3070-3089 Msa1343310:three_prime_UTR 35.0%
! CACCTTTTGCCTCTAATAAA+AGG + tig0006360:3072-3091 Msa1343310:three_prime_UTR 35.0%
! CTTTTATTAGAGGCAAAAGG+TGG - tig0006360:3070-3089 Msa1343320:intron 35.0%
GCAAAAGGTGGTTACATATA+GGG - tig0006360:3082-3101 Msa1343320:intron 35.0%
! TTATCTAAGGTCATGTTGGA+AGG + tig0006360:3161-3180 None:intergenic 35.0%
TTCCAACATGACCTTAGATA+AGG - tig0006360:3160-3179 Msa1343320:intron 35.0%
! TACCTTATCTAAGGTCATGT+TGG + tig0006360:3165-3184 None:intergenic 35.0%
ATTATTCTCCTATCAAGAGG+AGG + tig0006360:3660-3679 None:intergenic 35.0%
AATTTGAGAACACGGCTATA+AGG - tig0006360:3677-3696 Msa1343320:intron 35.0%
TGTGCTAAACGATGATGTAT+GGG + tig0006360:3856-3875 None:intergenic 35.0%
ATGTGCTAAACGATGATGTA+TGG + tig0006360:3857-3876 None:intergenic 35.0%
CAATGAATGAGGATTTGAAC+AGG + tig0006360:3934-3953 None:intergenic 35.0%
TGACCTCTTAGCAAGAAATA+TGG - tig0006360:2568-2587 Msa1343320:CDS 35.0%
AGGAGAAATTGAAAAAGGTG+TGG - tig0006360:2732-2751 Msa1343320:intron 35.0%
! TCATGATCTTGAAACCGTTT+TGG + tig0006360:2763-2782 Msa1343310:three_prime_UTR 35.0%
GTTTAACCTTTGTTGAGACA+CGG - tig0006360:2848-2867 Msa1343320:intron 35.0%
AATTAACCGTGTCTCAACAA+AGG + tig0006360:2857-2876 Msa1343310:three_prime_UTR 35.0%
GAATATTTGATGCATATGGG+TGG + tig0006360:3011-3030 Msa1343310:three_prime_UTR 35.0%
! ATGTAAGCCCCTTTTATTAG+AGG - tig0006360:3060-3079 Msa1343320:intron 35.0%
! CCTTTTGCCTCTAATAAAAG+GGG + tig0006360:3070-3089 Msa1343310:three_prime_UTR 35.0%
! CACCTTTTGCCTCTAATAAA+AGG + tig0006360:3072-3091 Msa1343310:three_prime_UTR 35.0%
! CTTTTATTAGAGGCAAAAGG+TGG - tig0006360:3070-3089 Msa1343320:intron 35.0%
GCAAAAGGTGGTTACATATA+GGG - tig0006360:3082-3101 Msa1343320:intron 35.0%
! TTATCTAAGGTCATGTTGGA+AGG + tig0006360:3161-3180 None:intergenic 35.0%
TTCCAACATGACCTTAGATA+AGG - tig0006360:3160-3179 Msa1343320:intron 35.0%
! TACCTTATCTAAGGTCATGT+TGG + tig0006360:3165-3184 None:intergenic 35.0%
ATTATTCTCCTATCAAGAGG+AGG + tig0006360:3660-3679 None:intergenic 35.0%
AATTTGAGAACACGGCTATA+AGG - tig0006360:3677-3696 Msa1343320:intron 35.0%
TGTGCTAAACGATGATGTAT+GGG + tig0006360:3856-3875 None:intergenic 35.0%
ATGTGCTAAACGATGATGTA+TGG + tig0006360:3857-3876 None:intergenic 35.0%
CAATGAATGAGGATTTGAAC+AGG + tig0006360:3934-3953 None:intergenic 35.0%
GAGCCATATTTCTTGCTAAG+AGG + tig0006360:2574-2593 Msa1343310:intron 40.0%
TATCAAGGAAGATCTTTGGG+AGG + tig0006360:2625-2644 Msa1343310:three_prime_UTR 40.0%
! CCAAAAGAGTGCATATGCTT+TGG + tig0006360:2658-2677 Msa1343310:three_prime_UTR 40.0%
! CCAAAGCATATGCACTCTTT+TGG - tig0006360:2655-2674 Msa1343320:CDS 40.0%
!! CACTCTTTTGGAAAGATGCA+TGG - tig0006360:2667-2686 Msa1343320:CDS 40.0%
AGATGCATGGTTAAGTTCCT+AGG - tig0006360:2680-2699 Msa1343320:intron 40.0%
ATTTGATGCATATGGGTGGT+AGG + tig0006360:3007-3026 Msa1343310:three_prime_UTR 40.0%
! CCCCTTTTATTAGAGGCAAA+AGG - tig0006360:3067-3086 Msa1343320:intron 40.0%
GGCAAAAGGTGGTTACATAT+AGG - tig0006360:3081-3100 Msa1343320:intron 40.0%
AAGGTGGTTACATATAGGGA+CGG - tig0006360:3086-3105 Msa1343320:intron 40.0%
TTGAGCAAGTCATCTTGAGA+AGG + tig0006360:3220-3239 None:intergenic 40.0%
! CCTAAAGCACCAACATAAAC+TGG + tig0006360:3272-3291 None:intergenic 40.0%
! CCAGTTTATGTTGGTGCTTT+AGG - tig0006360:3269-3288 Msa1343320:intron 40.0%
CTTATGCTGCAAGCTGTAAT+AGG + tig0006360:3468-3487 None:intergenic 40.0%
GAATGAGGATTTGAACAGGT+GGG + tig0006360:3930-3949 None:intergenic 40.0%
TGAATGAGGATTTGAACAGG+TGG + tig0006360:3931-3950 None:intergenic 40.0%
GAGCCATATTTCTTGCTAAG+AGG + tig0006360:2574-2593 Msa1343310:intron 40.0%
TATCAAGGAAGATCTTTGGG+AGG + tig0006360:2625-2644 Msa1343310:three_prime_UTR 40.0%
! CCAAAAGAGTGCATATGCTT+TGG + tig0006360:2658-2677 Msa1343310:three_prime_UTR 40.0%
! CCAAAGCATATGCACTCTTT+TGG - tig0006360:2655-2674 Msa1343320:CDS 40.0%
!! CACTCTTTTGGAAAGATGCA+TGG - tig0006360:2667-2686 Msa1343320:CDS 40.0%
AGATGCATGGTTAAGTTCCT+AGG - tig0006360:2680-2699 Msa1343320:intron 40.0%
ATTTGATGCATATGGGTGGT+AGG + tig0006360:3007-3026 Msa1343310:three_prime_UTR 40.0%
! CCCCTTTTATTAGAGGCAAA+AGG - tig0006360:3067-3086 Msa1343320:intron 40.0%
GGCAAAAGGTGGTTACATAT+AGG - tig0006360:3081-3100 Msa1343320:intron 40.0%
AAGGTGGTTACATATAGGGA+CGG - tig0006360:3086-3105 Msa1343320:intron 40.0%
TTGAGCAAGTCATCTTGAGA+AGG + tig0006360:3220-3239 None:intergenic 40.0%
! CCTAAAGCACCAACATAAAC+TGG + tig0006360:3272-3291 None:intergenic 40.0%
! CCAGTTTATGTTGGTGCTTT+AGG - tig0006360:3269-3288 Msa1343320:intron 40.0%
CTTATGCTGCAAGCTGTAAT+AGG + tig0006360:3468-3487 None:intergenic 40.0%
GAATGAGGATTTGAACAGGT+GGG + tig0006360:3930-3949 None:intergenic 40.0%
TGAATGAGGATTTGAACAGG+TGG + tig0006360:3931-3950 None:intergenic 40.0%
AAGGTGTGGAAGCTCCAAAA+CGG - tig0006360:2746-2765 Msa1343320:intron 45.0%
! GAAGTCTAAGGCGATTGTCT+TGG - tig0006360:2949-2968 Msa1343320:intron 45.0%
GGCTTACATATAGGGACAGA+GGG + tig0006360:3049-3068 Msa1343310:three_prime_UTR 45.0%
GCTAAACGATGATGTATGGG+CGG + tig0006360:3853-3872 None:intergenic 45.0%
TCGTTTAGCACATCCTCTTG+TGG - tig0006360:3864-3883 Msa1343320:CDS 45.0%
CATAAGTCACTCTCCACAAG+AGG + tig0006360:3880-3899 None:intergenic 45.0%
AAGGTGTGGAAGCTCCAAAA+CGG - tig0006360:2746-2765 Msa1343320:intron 45.0%
! GAAGTCTAAGGCGATTGTCT+TGG - tig0006360:2949-2968 Msa1343320:intron 45.0%
GGCTTACATATAGGGACAGA+GGG + tig0006360:3049-3068 Msa1343310:three_prime_UTR 45.0%
GCTAAACGATGATGTATGGG+CGG + tig0006360:3853-3872 None:intergenic 45.0%
TCGTTTAGCACATCCTCTTG+TGG - tig0006360:3864-3883 Msa1343320:CDS 45.0%
CATAAGTCACTCTCCACAAG+AGG + tig0006360:3880-3899 None:intergenic 45.0%
! GTCTAAGGCGATTGTCTTGG+TGG - tig0006360:2952-2971 Msa1343320:intron 50.0%
GATGCATATGGGTGGTAGGA+AGG + tig0006360:3003-3022 Msa1343310:three_prime_UTR 50.0%
GGGCTTACATATAGGGACAG+AGG + tig0006360:3050-3069 Msa1343310:three_prime_UTR 50.0%
GTGGTTACATATAGGGACGG+CGG - tig0006360:3089-3108 Msa1343320:intron 50.0%
TGGTTACATATAGGGACGGC+GGG - tig0006360:3090-3109 Msa1343320:intron 50.0%
!! TTGGTTGGCCTCCTCTTGAT+AGG - tig0006360:3649-3668 Msa1343320:intron 50.0%
! ATGCTGTTTGTGCGATGTCG+CGG + tig0006360:3906-3925 None:intergenic 50.0%
! GTCTAAGGCGATTGTCTTGG+TGG - tig0006360:2952-2971 Msa1343320:intron 50.0%
GATGCATATGGGTGGTAGGA+AGG + tig0006360:3003-3022 Msa1343310:three_prime_UTR 50.0%
GGGCTTACATATAGGGACAG+AGG + tig0006360:3050-3069 Msa1343310:three_prime_UTR 50.0%
GTGGTTACATATAGGGACGG+CGG - tig0006360:3089-3108 Msa1343320:intron 50.0%
TGGTTACATATAGGGACGGC+GGG - tig0006360:3090-3109 Msa1343320:intron 50.0%
!! TTGGTTGGCCTCCTCTTGAT+AGG - tig0006360:3649-3668 Msa1343320:intron 50.0%
! ATGCTGTTTGTGCGATGTCG+CGG + tig0006360:3906-3925 None:intergenic 50.0%
TGGGTGGTAGGAAGGAGTCA+GGG + tig0006360:2995-3014 Msa1343310:three_prime_UTR 55.0%
ATGGGTGGTAGGAAGGAGTC+AGG + tig0006360:2996-3015 Msa1343310:three_prime_UTR 55.0%
CGGCGGGAGTAGAAAACATG+TGG - tig0006360:3106-3125 Msa1343320:intron 55.0%
AACGATGATGTATGGGCGGC+AGG + tig0006360:3849-3868 None:intergenic 55.0%
TGGGTGGTAGGAAGGAGTCA+GGG + tig0006360:2995-3014 Msa1343310:three_prime_UTR 55.0%
ATGGGTGGTAGGAAGGAGTC+AGG + tig0006360:2996-3015 Msa1343310:three_prime_UTR 55.0%
CGGCGGGAGTAGAAAACATG+TGG - tig0006360:3106-3125 Msa1343320:intron 55.0%
AACGATGATGTATGGGCGGC+AGG + tig0006360:3849-3868 None:intergenic 55.0%
!! TGGTGGTGCTACCCTCTGAC+CGG - tig0006360:2969-2988 Msa1343320:intron 60.0%
TGGTAGGAAGGAGTCAGGGC+CGG + tig0006360:2991-3010 Msa1343310:three_prime_UTR 60.0%
CTGTTTGTGCGATGTCGCGG+AGG + tig0006360:3903-3922 None:intergenic 60.0%
!! TGGTGGTGCTACCCTCTGAC+CGG - tig0006360:2969-2988 Msa1343320:intron 60.0%
TGGTAGGAAGGAGTCAGGGC+CGG + tig0006360:2991-3010 Msa1343310:three_prime_UTR 60.0%
CTGTTTGTGCGATGTCGCGG+AGG + tig0006360:3903-3922 None:intergenic 60.0%
AGGAGTCAGGGCCGGTCAGA+GGG + tig0006360:2983-3002 Msa1343310:three_prime_UTR 65.0%
AAGGAGTCAGGGCCGGTCAG+AGG + tig0006360:2984-3003 Msa1343310:three_prime_UTR 65.0%
AGGAGTCAGGGCCGGTCAGA+GGG + tig0006360:2983-3002 Msa1343310:three_prime_UTR 65.0%
AAGGAGTCAGGGCCGGTCAG+AGG + tig0006360:2984-3003 Msa1343310:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0006360 gene 2540 3969 2540 ID=Msa1343320;Name=Msa1343320
tig0006360 mRNA 2540 3969 2540 ID=Msa1343320-mRNA-1;Parent=Msa1343320;Name=Msa1343320-mRNA-1;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.33|1|1|3|0|100
tig0006360 exon 3965 3969 3965 ID=Msa1343320-mRNA-1:exon:4116;Parent=Msa1343320-mRNA-1
tig0006360 exon 3808 3953 3808 ID=Msa1343320-mRNA-1:exon:4115;Parent=Msa1343320-mRNA-1
tig0006360 exon 2540 2691 2540 ID=Msa1343320-mRNA-1:exon:4114;Parent=Msa1343320-mRNA-1
tig0006360 CDS 3965 3969 3965 ID=Msa1343320-mRNA-1:cds;Parent=Msa1343320-mRNA-1
tig0006360 CDS 3808 3953 3808 ID=Msa1343320-mRNA-1:cds;Parent=Msa1343320-mRNA-1
tig0006360 CDS 2540 2691 2540 ID=Msa1343320-mRNA-1:cds;Parent=Msa1343320-mRNA-1
Gene Sequence

>Msa1343320

ATGATCAACAGTGAGAATGACCTCTTAGCAAGAAATATGGCTCCCATAGAACATGAGAATGATAACAGACTCCTCCCAAAGATCTTCCTTGATAACAATGTTTTCCAAAGCATATGCACTCTTTTGGAAAGATGCATGGTTAAGTTCCTAGATTTTCCAGAAATGATAAGGTTGCTAAACCTGCCGCCCATACATCATCGTTTAGCACATCCTCTTGTGGAGAGTGACTTATGCCTCCGCGACATCGCACAAACAGCATTCCCACCTGTTCAAATCCTCATTCATTGTCATATTCTAACATAA
Protein sequence

>Msa1343320

MINSENDLLARNMAPIEHENDNRLLPKIFLDNNVFQSICTLLERCMVKFLDFPEMIRLLNLPPIHHRLAHPLVESDLCLRDIAQTAFPPVQILIHCHILT*