Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0637070 | KEH17609.1 | 95.082 | 61 | 3 | 0 | 1 | 61 | 4 | 64 | 1.66e-34 | 123 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0637070 | A0A072TJ66 | 95.082 | 61 | 3 | 0 | 1 | 61 | 4 | 64 | 7.94e-35 | 123 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025740 | Msa0637070 | 0.828354 | 9.420961e-55 | -8.615850e-47 |
| Msa0062860 | Msa0637070 | 0.809746 | 1.624508e-50 | -8.615850e-47 |
| Msa0089360 | Msa0637070 | 0.800338 | 1.510425e-48 | -8.615850e-47 |
| Msa0092990 | Msa0637070 | 0.833226 | 6.024666e-56 | -8.615850e-47 |
| Msa0096450 | Msa0637070 | 0.821512 | 3.881330e-53 | -8.615850e-47 |
| Msa0100000 | Msa0637070 | 0.804781 | 1.831710e-49 | -8.615850e-47 |
| Msa0112750 | Msa0637070 | 0.822600 | 2.172369e-53 | -8.615850e-47 |
| Msa0115810 | Msa0637070 | 0.803073 | 4.149066e-49 | -8.615850e-47 |
| Msa0117770 | Msa0637070 | 0.817749 | 2.806710e-52 | -8.615850e-47 |
| Msa0120340 | Msa0637070 | 0.813930 | 1.994311e-51 | -8.615850e-47 |
| Msa0610590 | Msa0637070 | 0.809138 | 2.194752e-50 | -8.615850e-47 |
| Msa0627350 | Msa0637070 | 0.801145 | 1.033789e-48 | -8.615850e-47 |
| Msa0637070 | Msa0649130 | 0.802958 | 4.382162e-49 | -8.615850e-47 |
| Msa0637070 | Msa0651190 | 0.813559 | 2.407323e-51 | -8.615850e-47 |
| Msa0637070 | Msa0660300 | 0.802143 | 6.453613e-49 | -8.615850e-47 |
| Msa0637070 | Msa0661870 | 0.814089 | 1.840069e-51 | -8.615850e-47 |
| Msa0637070 | Msa0662650 | 0.833700 | 4.586170e-56 | -8.615850e-47 |
| Msa0637070 | Msa0682550 | 0.803620 | 3.195972e-49 | -8.615850e-47 |
| Msa0637070 | Msa0688070 | 0.801367 | 9.311429e-49 | -8.615850e-47 |
| Msa0637070 | Msa0693380 | 0.800682 | 1.285242e-48 | -8.615850e-47 |
| Msa0637070 | Msa0696640 | 0.818640 | 1.764052e-52 | -8.615850e-47 |
| Msa0637070 | Msa0708860 | 0.836772 | 7.692541e-57 | -8.615850e-47 |
| Msa0637070 | Msa0715470 | 0.832230 | 1.064117e-55 | -8.615850e-47 |
| Msa0637070 | Msa0731180 | 0.822678 | 2.082820e-53 | -8.615850e-47 |
| Msa0637070 | Msa0757080 | 0.812357 | 4.414845e-51 | -8.615850e-47 |
| Msa0637070 | Msa0766630 | 0.809736 | 1.633083e-50 | -8.615850e-47 |
| Msa0637070 | Msa0771580 | 0.803568 | 3.276494e-49 | -8.615850e-47 |
| Msa0637070 | Msa0795080 | 0.829551 | 4.832223e-55 | -8.615850e-47 |
| Msa0637070 | Msa0827470 | 0.818563 | 1.836471e-52 | -8.615850e-47 |
| Msa0637070 | Msa0846810 | 0.804863 | 1.761134e-49 | -8.615850e-47 |
| Msa0637070 | Msa0847880 | 0.821690 | 3.530229e-53 | -8.615850e-47 |
| Msa0637070 | Msa0860410 | 0.825841 | 3.762567e-54 | -8.615850e-47 |
| Msa0637070 | Msa0899230 | 0.816997 | 4.143725e-52 | -8.615850e-47 |
| Msa0637070 | Msa0907070 | 0.833120 | 6.401179e-56 | -8.615850e-47 |
| Msa0637070 | Msa0908170 | 0.827232 | 1.753096e-54 | -8.615850e-47 |
| Msa0637070 | Msa0910980 | -0.823235 | 1.544820e-53 | -8.615850e-47 |
| Msa0637070 | Msa0927670 | 0.819731 | 9.954565e-53 | -8.615850e-47 |
| Msa0637070 | Msa0960160 | 0.828042 | 1.120158e-54 | -8.615850e-47 |
| Msa0637070 | Msa0961420 | -0.807868 | 4.096374e-50 | -8.615850e-47 |
| Msa0637070 | Msa0965750 | 0.835897 | 1.284411e-56 | -8.615850e-47 |
| Msa0637070 | Msa0990960 | 0.802503 | 5.439304e-49 | -8.615850e-47 |
| Msa0637070 | Msa0999040 | 0.826627 | 2.445551e-54 | -8.615850e-47 |
| Msa0637070 | Msa1039880 | 0.837983 | 3.765959e-57 | -8.615850e-47 |
| Msa0637070 | Msa1073070 | -0.808601 | 2.858119e-50 | -8.615850e-47 |
| Msa0637070 | Msa1082500 | 0.849098 | 4.022146e-60 | -8.615850e-47 |
| Msa0637070 | Msa1082580 | 0.806100 | 9.692528e-50 | -8.615850e-47 |
| Msa0637070 | Msa1092090 | 0.808081 | 3.689810e-50 | -8.615850e-47 |
| Msa0637070 | Msa1098400 | 0.804480 | 2.116891e-49 | -8.615850e-47 |
| Msa0637070 | Msa1111180 | 0.801021 | 1.095815e-48 | -8.615850e-47 |
| Msa0637070 | Msa1130270 | -0.800101 | 1.687632e-48 | -8.615850e-47 |
| Msa0637070 | Msa1172670 | 0.817973 | 2.497663e-52 | -8.615850e-47 |
| Msa0637070 | Msa1179460 | 0.823207 | 1.568234e-53 | -8.615850e-47 |
| Msa0637070 | Msa1201120 | 0.807854 | 4.123517e-50 | -8.615850e-47 |
| Msa0637070 | Msa1224480 | 0.801544 | 8.565775e-49 | -8.615850e-47 |
| Msa0637070 | Msa1233500 | 0.841291 | 5.193165e-58 | -8.615850e-47 |
| Msa0637070 | Msa1233890 | 0.808561 | 2.915375e-50 | -8.615850e-47 |
| Msa0637070 | Msa1256380 | 0.800426 | 1.449521e-48 | -8.615850e-47 |
| Msa0637070 | Msa1275770 | 0.842254 | 2.892251e-58 | -8.615850e-47 |
| Msa0637070 | Msa1276440 | 0.834342 | 3.167703e-56 | -8.615850e-47 |
| Msa0637070 | Msa1296940 | 0.801110 | 1.050889e-48 | -8.615850e-47 |
| Msa0637070 | Msa1298260 | 0.802351 | 5.846133e-49 | -8.615850e-47 |
| Msa0637070 | Msa1299070 | 0.811080 | 8.373917e-51 | -8.615850e-47 |
| Msa0637070 | Msa1306750 | 0.815498 | 8.962809e-52 | -8.615850e-47 |
| Msa0637070 | Msa1308590 | 0.828058 | 1.110417e-54 | -8.615850e-47 |
| Msa0637070 | Msa1308640 | 0.819620 | 1.055551e-52 | -8.615850e-47 |
| Msa0637070 | Msa1314340 | 0.829157 | 6.023832e-55 | -8.615850e-47 |
| Msa0637070 | Msa1322190 | 0.805688 | 1.183200e-49 | -8.615850e-47 |
| Msa0637070 | Msa1343320 | 0.823617 | 1.258078e-53 | -8.615850e-47 |
| Msa0637070 | Msa1348950 | 0.826730 | 2.310806e-54 | -8.615850e-47 |
| Msa0637070 | Msa1362880 | 0.813091 | 3.049818e-51 | -8.615850e-47 |
| Msa0637070 | Msa1393040 | 0.817338 | 3.472429e-52 | -8.615850e-47 |
| Msa0637070 | Msa1397610 | 0.804676 | 1.926728e-49 | -8.615850e-47 |
| Msa0637070 | Msa1407600 | 0.817996 | 2.468110e-52 | -8.615850e-47 |
| Msa0637070 | Msa1411020 | 0.807492 | 4.922481e-50 | -8.615850e-47 |
| Msa0637070 | Msa1420140 | 0.851929 | 6.441008e-61 | -8.615850e-47 |
| Msa0637070 | Msa1424290 | 0.805556 | 1.261149e-49 | -8.615850e-47 |
| Msa0637070 | Msa1424680 | 0.800719 | 1.263338e-48 | -8.615850e-47 |
| Msa0637070 | Msa1425100 | 0.841249 | 5.328892e-58 | -8.615850e-47 |
| Msa0637070 | Msa1434350 | 0.807620 | 4.624138e-50 | -8.615850e-47 |
| Msa0637070 | Msa1439320 | 0.810810 | 9.577961e-51 | -8.615850e-47 |
| Msa0637070 | Msa1445030 | 0.815562 | 8.673724e-52 | -8.615850e-47 |
| Msa0637070 | Msa1451620 | -0.823558 | 1.298657e-53 | -8.615850e-47 |
| Msa0637070 | Msa1463670 | 0.803082 | 4.130533e-49 | -8.615850e-47 |
| Msa0637070 | Msa1466730 | 0.822372 | 2.454396e-53 | -8.615850e-47 |
| Msa0121620 | Msa0637070 | 0.817304 | 3.534194e-52 | -8.615850e-47 |
| Msa0122590 | Msa0637070 | 0.813897 | 2.028179e-51 | -8.615850e-47 |
| Msa0161900 | Msa0637070 | 0.800695 | 1.277414e-48 | -8.615850e-47 |
| Msa0205850 | Msa0637070 | 0.800369 | 1.488703e-48 | -8.615850e-47 |
| Msa0233300 | Msa0637070 | 0.803089 | 4.116270e-49 | -8.615850e-47 |
| Msa0365490 | Msa0637070 | 0.825674 | 4.120885e-54 | -8.615850e-47 |
| Msa0376590 | Msa0637070 | 0.800702 | 1.273444e-48 | -8.615850e-47 |
| Msa0379640 | Msa0637070 | 0.816995 | 4.147081e-52 | -8.615850e-47 |
| Msa0431690 | Msa0637070 | 0.836547 | 8.777679e-57 | -8.615850e-47 |
| Msa0444090 | Msa0637070 | 0.835039 | 2.115606e-56 | -8.615850e-47 |
| Msa0458440 | Msa0637070 | 0.829237 | 5.761101e-55 | -8.615850e-47 |
| Msa0464900 | Msa0637070 | 0.817017 | 4.100638e-52 | -8.615850e-47 |
| Msa0465680 | Msa0637070 | -0.801316 | 9.535136e-49 | -8.615850e-47 |
| Msa0466390 | Msa0637070 | 0.804523 | 2.074053e-49 | -8.615850e-47 |
| Msa0473240 | Msa0637070 | 0.801934 | 7.122516e-49 | -8.615850e-47 |
| Msa0243710 | Msa0637070 | 0.813158 | 2.949426e-51 | -8.615850e-47 |
| Msa0245940 | Msa0637070 | 0.800584 | 1.345593e-48 | -8.615850e-47 |
| Msa0258760 | Msa0637070 | 0.826237 | 3.028738e-54 | -8.615850e-47 |
| Msa0269450 | Msa0637070 | 0.835308 | 1.810158e-56 | -8.615850e-47 |
| Msa0284520 | Msa0637070 | 0.800519 | 1.387488e-48 | -8.615850e-47 |
| Msa0296560 | Msa0637070 | 0.814492 | 1.498512e-51 | -8.615850e-47 |
| Msa0323080 | Msa0637070 | 0.823466 | 1.364041e-53 | -8.615850e-47 |
| Msa0335040 | Msa0637070 | 0.810942 | 8.967325e-51 | -8.615850e-47 |
| Msa0344830 | Msa0637070 | 0.807497 | 4.910507e-50 | -8.615850e-47 |
| Msa0350670 | Msa0637070 | 0.804574 | 2.023820e-49 | -8.615850e-47 |
| Msa0350990 | Msa0637070 | 0.814354 | 1.607722e-51 | -8.615850e-47 |
| Msa0351030 | Msa0637070 | 0.814787 | 1.289501e-51 | -8.615850e-47 |
| Msa0351810 | Msa0637070 | 0.815795 | 7.696465e-52 | -8.615850e-47 |
| Msa0484780 | Msa0637070 | 0.857136 | 2.004909e-62 | -8.615850e-47 |
| Msa0491520 | Msa0637070 | 0.811355 | 7.296023e-51 | -8.615850e-47 |
| Msa0512900 | Msa0637070 | 0.821119 | 4.782028e-53 | -8.615850e-47 |
| Msa0530650 | Msa0637070 | 0.806915 | 6.522983e-50 | -8.615850e-47 |
| Msa0536220 | Msa0637070 | 0.803600 | 3.225773e-49 | -8.615850e-47 |
| Msa0553460 | Msa0637070 | 0.804243 | 2.371628e-49 | -8.615850e-47 |
| Msa0558690 | Msa0637070 | 0.801001 | 1.106111e-48 | -8.615850e-47 |
| Msa0562440 | Msa0637070 | 0.816357 | 5.764638e-52 | -8.615850e-47 |
| Msa0578970 | Msa0637070 | 0.805825 | 1.107111e-49 | -8.615850e-47 |
| Msa0599580 | Msa0637070 | 0.827757 | 1.311236e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0637070 | MtrunA17_Chr4g0020511 | 95.082 | 61 | 3 | 0 | 1 | 61 | 4 | 64 | 1.53e-38 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 4 sgRNAs with CRISPR-Local
Find 7 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGATTGAGTGCATTTATA+AGG | 0.265431 | 4_3:+28330843 | Msa0637070:CDS |
| CATTTATAAGGATGATGTCT+TGG | 0.472239 | 4_3:+28330855 | Msa0637070:CDS |
| TCATCACATTAATAAAGCTA+TGG | 0.498164 | 4_3:+28330783 | Msa0637070:CDS |
| CATCACATTAATAAAGCTAT+GGG | 0.538615 | 4_3:+28330784 | Msa0637070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCATCACATTAATAAAGCTA+TGG | + | chr4_3:28330783-28330802 | Msa0637070:CDS | 25.0% |
| ! | CATCACATTAATAAAGCTAT+GGG | + | chr4_3:28330784-28330803 | Msa0637070:CDS | 25.0% |
| ! | AATGATTGAGTGCATTTATA+AGG | + | chr4_3:28330843-28330862 | Msa0637070:CDS | 25.0% |
| CATTTATAAGGATGATGTCT+TGG | + | chr4_3:28330855-28330874 | Msa0637070:CDS | 30.0% | |
| ! | CGAAAGGTTTTGCTTAGAAA+AGG | - | chr4_3:28330752-28330771 | None:intergenic | 35.0% |
| ! | GATGAAGTTTTTGCATCGAA+AGG | - | chr4_3:28330768-28330787 | None:intergenic | 35.0% |
| ! | ATGGGAGATGACGTATTTTA+TGG | + | chr4_3:28330802-28330821 | Msa0637070:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 28330730 | 28330951 | 28330730 | ID=Msa0637070;Name=Msa0637070 |
| chr4_3 | mRNA | 28330730 | 28330951 | 28330730 | ID=Msa0637070-mRNA-1;Parent=Msa0637070;Name=Msa0637070-mRNA-1;_AED=0.12;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|73 |
| chr4_3 | exon | 28330730 | 28330951 | 28330730 | ID=Msa0637070-mRNA-1:exon:6974;Parent=Msa0637070-mRNA-1 |
| chr4_3 | CDS | 28330730 | 28330951 | 28330730 | ID=Msa0637070-mRNA-1:cds;Parent=Msa0637070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
