AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393330 MtrunA17_Chr2g0322361 95.333 750 34 1 1 749 1 750 0.0 1456
Msa1393330 MtrunA17_Chr2g0322371 86.933 750 87 4 1 749 1 740 0.0 1337
Msa1393330 MtrunA17_Chr4g0025201 58.211 749 303 7 1 747 1 741 0.0 881
Msa1393330 MtrunA17_Chr2g0322351 57.636 753 295 10 1 747 1 735 0.0 848
Msa1393330 MtrunA17_Chr2g0322341 65.230 348 115 4 1 346 3 346 4.58e-160 467
Msa1393330 MtrunA17_Chr3g0102531 33.955 751 453 14 1 737 1 722 4.03e-137 421
Msa1393330 MtrunA17_Chr4g0073711 34.783 805 439 18 1 739 228 1012 9.76e-137 429
Msa1393330 MtrunA17_Chr8g0379241 33.974 780 442 16 21 739 196 963 2.20e-134 422
Msa1393330 MtrunA17_Chr2g0284211 33.376 785 443 15 21 739 181 951 5.62e-134 421
Msa1393330 MtrunA17_Chr3g0102541 34.214 757 447 17 1 737 1 726 2.42e-133 412
Msa1393330 MtrunA17_Chr3g0102551 34.556 709 411 14 50 740 60 733 6.42e-133 411
Msa1393330 MtrunA17_Chr3g0137951 33.822 819 446 19 1 739 258 1060 6.58e-133 420
Msa1393330 MtrunA17_Chr1g0183381 34.003 797 450 20 1 739 100 878 3.88e-132 414
Msa1393330 MtrunA17_Chr8g0364641 34.761 794 443 22 1 739 182 955 6.29e-132 415
Msa1393330 MtrunA17_Chr8g0352231 33.758 785 425 18 34 739 284 1052 3.19e-131 416
Msa1393330 MtrunA17_Chr2g0322331 52.987 385 167 6 375 749 1 381 4.24e-130 391
Msa1393330 MtrunA17_Chr1g0201731 32.681 817 457 16 2 739 269 1071 1.32e-128 409
Msa1393330 MtrunA17_Chr1g0186381 32.383 772 442 20 2 739 4 729 7.69e-111 353
Msa1393330 MtrunA17_Chr5g0410641 30.260 846 438 24 8 719 252 1079 1.69e-108 356
Msa1393330 MtrunA17_Chr4g0046401 31.694 732 446 17 34 744 37 735 1.92e-107 344
Msa1393330 MtrunA17_Chr1g0165411 30.414 822 434 22 18 719 258 1061 8.57e-107 351
Msa1393330 MtrunA17_Chr2g0298581 31.397 723 442 18 16 723 8 691 1.10e-106 341
Msa1393330 MtrunA17_Chr1g0165421 30.542 848 436 25 8 719 259 1089 3.48e-106 350
Msa1393330 MtrunA17_Chr4g0039801 30.769 845 435 27 8 719 273 1100 2.75e-105 348
Msa1393330 MtrunA17_Chr2g0297351 29.440 839 450 22 8 719 159 982 4.61e-105 345
Msa1393330 MtrunA17_Chr2g0307191 30.685 730 434 16 16 721 8 689 5.55e-105 336
Msa1393330 MtrunA17_Chr3g0095791 29.888 716 457 17 16 721 8 688 2.08e-104 335
Msa1393330 MtrunA17_Chr3g0077511 30.435 667 423 12 16 672 8 643 1.37e-102 330
Msa1393330 MtrunA17_Chr7g0269741 29.621 871 452 24 8 738 297 1146 1.21e-101 338
Msa1393330 MtrunA17_Chr8g0383701 42.172 396 206 10 1 377 266 657 9.25e-88 299
Msa1393330 MtrunA17_Chr8g0383701 30.435 368 237 5 381 739 713 1070 2.56e-41 163
Msa1393330 MtrunA17_Chr3g0088821 40.967 393 211 10 3 377 250 639 6.44e-87 296
Msa1393330 MtrunA17_Chr3g0088821 30.986 355 226 7 391 739 711 1052 5.07e-41 162
Msa1393330 MtrunA17_Chr2g0296991 41.667 396 214 7 1 380 199 593 3.43e-86 294
Msa1393330 MtrunA17_Chr2g0296991 30.618 356 225 8 391 739 678 1018 5.08e-40 159
Msa1393330 MtrunA17_Chr7g0274751 29.195 733 357 24 1 649 89 743 1.22e-84 285
Msa1393330 MtrunA17_Chr3g0078671 41.162 396 212 10 1 378 215 607 1.08e-83 287
Msa1393330 MtrunA17_Chr3g0078671 30.110 362 238 7 391 746 679 1031 2.14e-38 154
Msa1393330 MtrunA17_Chr7g0267731 39.286 392 219 9 3 377 162 551 7.32e-83 283
Msa1393330 MtrunA17_Chr7g0267731 30.495 364 224 7 387 739 615 960 4.64e-42 165
Msa1393330 MtrunA17_Chr2g0307241 38.462 182 104 3 16 192 8 186 1.39e-33 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393330 AT2G32530.1 46.103 757 373 10 8 743 10 752 0.0 660
Msa1393330 AT2G32540.1 46.499 757 370 11 8 743 10 752 0.0 639
Msa1393330 AT4G15320.3 44.823 763 380 13 7 743 9 756 0.0 627
Msa1393330 AT4G15320.5 44.751 762 380 13 7 742 9 755 0.0 625
Msa1393330 AT4G15320.4 43.959 778 380 14 7 743 9 771 0.0 619
Msa1393330 AT2G32620.1 46.174 758 373 12 8 743 10 754 0.0 617
Msa1393330 AT2G32610.1 43.495 761 391 13 8 743 10 756 0.0 604
Msa1393330 AT4G15290.1 44.327 758 385 12 8 742 10 753 0.0 585
Msa1393330 AT2G32620.4 45.495 666 333 10 100 743 1 658 0.0 548
Msa1393330 AT2G32620.3 47.342 602 294 8 8 599 10 598 0.0 531
Msa1393330 AT4G15320.2 41.954 696 319 12 7 646 9 675 1.58e-178 530
Msa1393330 AT4G15320.1 39.648 739 318 14 7 646 9 718 7.29e-170 509
Msa1393330 AT2G32620.2 48.956 527 243 8 8 522 10 522 2.62e-162 480
Msa1393330 AT5G17420.1 34.919 799 447 15 1 739 215 1000 8.21e-142 442
Msa1393330 AT5G09870.1 34.061 825 448 17 1 743 241 1051 1.54e-137 432
Msa1393330 AT5G05170.1 34.474 818 439 18 3 739 238 1039 5.04e-137 431
Msa1393330 AT4G32410.1 34.069 816 448 19 1 739 252 1054 1.10e-136 431
Msa1393330 AT4G18780.1 34.552 793 436 16 16 739 179 957 2.00e-136 427
Msa1393330 AT1G55850.1 37.083 720 386 18 37 737 49 720 2.01e-136 420
Msa1393330 AT5G64740.1 33.576 825 455 18 1 743 253 1066 4.50e-136 429
Msa1393330 AT2G25540.1 34.069 816 448 20 1 739 232 1034 1.03e-134 425
Msa1393330 AT2G21770.1 33.130 821 461 16 1 743 259 1069 1.08e-130 415
Msa1393330 AT4G23990.1 32.668 701 407 18 62 740 86 743 7.37e-110 351
Msa1393330 AT4G24000.1 33.425 724 416 19 35 739 37 713 2.21e-109 349
Msa1393330 AT4G24010.1 33.147 715 404 18 35 723 40 706 1.69e-107 345
Msa1393330 AT1G32180.1 30.630 826 436 22 25 720 123 941 3.11e-107 350
Msa1393330 AT1G32180.2 30.630 826 436 22 25 720 123 941 3.11e-107 350
Msa1393330 AT2G33100.2 29.405 840 455 20 8 719 167 996 2.92e-104 343
Msa1393330 AT2G33100.1 29.405 840 455 20 8 719 167 996 2.92e-104 343
Msa1393330 AT4G38190.1 30.387 826 444 24 8 719 256 1064 9.38e-104 343
Msa1393330 AT3G03050.1 30.486 843 438 25 8 719 278 1103 1.90e-102 340
Msa1393330 AT1G02730.1 29.875 877 449 26 8 738 302 1158 1.43e-99 333
Msa1393330 AT5G16910.1 29.608 841 445 24 8 719 281 1103 1.08e-97 327
Msa1393330 AT4G39350.1 41.667 396 210 9 1 378 254 646 3.73e-87 297
Msa1393330 AT4G39350.1 29.006 362 243 4 387 743 713 1065 6.36e-37 149
Msa1393330 AT5G44030.1 41.944 391 206 10 8 380 199 586 9.19e-85 290
Msa1393330 AT5G44030.1 30.137 365 235 8 381 739 673 1023 7.44e-38 152
Msa1393330 AT5G44030.2 41.944 391 206 10 8 380 228 615 1.43e-84 290
Msa1393330 AT5G44030.2 30.137 365 235 8 381 739 702 1052 6.84e-38 152
Msa1393330 AT5G44030.3 45.667 300 151 6 92 380 9 307 3.06e-76 262
Msa1393330 AT5G44030.3 30.137 365 235 8 381 739 394 744 2.45e-38 153
Msa1393330 AT4G24000.2 32.021 559 321 15 199 738 2 520 3.73e-72 245
Msa1393330 AT4G23990.2 30.986 568 326 16 199 740 2 529 2.38e-71 243

Find 150 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGGTAAAATCCAATTT+AGG 0.109508 tig0021657:+73169 Msa1393330:CDS
AGCACTTGTTGTTAAGATTT+TGG 0.116121 tig0021657:+73820 Msa1393330:CDS
TACCCAAAACTTAGCAAATT+TGG 0.129500 tig0021657:-71185 None:intergenic
AAGGATTATCATTAAGATTT+TGG 0.147084 tig0021657:+73570 Msa1393330:CDS
AGTATTAGTCTTGAGATTAA+TGG 0.186408 tig0021657:+74050 Msa1393330:three_prime_UTR
TCAACAAGTGTATGGTTTAT+TGG 0.197802 tig0021657:+73623 Msa1393330:CDS
CTTCCAAATTTGCTAAGTTT+TGG 0.203327 tig0021657:+71182 Msa1393330:CDS
CATATATAGCATTTCTTCTA+TGG 0.224796 tig0021657:-72329 None:intergenic
GGAATTAAAGATGACCCTTT+TGG 0.230566 tig0021657:+72133 Msa1393330:CDS
CCTGTTATGGGTACCCTCTT+TGG 0.238815 tig0021657:+73153 Msa1393330:CDS
CTTCAGATGGGCTGGTTATA+TGG 0.244829 tig0021657:+72949 Msa1393330:intron
AAGACAAGCCAGCCCTAAAT+TGG 0.251195 tig0021657:-73182 None:intergenic
AACCATTCTTGTTTAAATTC+TGG 0.253368 tig0021657:-71286 None:intergenic
TTGTTTGCTAGGGGAAAATA+TGG 0.258969 tig0021657:+73941 Msa1393330:CDS
CTTATTGTTGGCTTACCAAT+TGG 0.259289 tig0021657:+73202 Msa1393330:CDS
TAGCAAGCATTCTCCTGTTA+TGG 0.266654 tig0021657:+73140 Msa1393330:CDS
AAGATATTAATACAGCAATT+TGG 0.270520 tig0021657:+72494 Msa1393330:CDS
CTTTGGTAAAATCCAATTTA+GGG 0.273325 tig0021657:+73170 Msa1393330:CDS
TTGCTTTCTCATGTGAATCT+TGG 0.274164 tig0021657:+70373 Msa1393330:CDS
TACACGTTGGCAGGTTATAT+TGG 0.291130 tig0021657:+71749 Msa1393330:intron
AGTGGCATGATTAAGCTCTT+AGG 0.292985 tig0021657:+73653 Msa1393330:CDS
CTTTATGCAGTACATAATAA+AGG 0.297679 tig0021657:+72260 Msa1393330:intron
TGTATCTAGTAGTATGTTAT+TGG 0.305072 tig0021657:+73903 Msa1393330:CDS
AACAAAGAAGAGAAAATAAA+AGG 0.305339 tig0021657:-70299 None:intergenic
CCGCATATTCTCCCTCAAAT+TGG 0.312179 tig0021657:-71472 None:intergenic
GCTTGTTATGTTTCTGATGA+TGG 0.315570 tig0021657:+71124 Msa1393330:CDS
GCTGTCTTATTCCAGGGACT+TGG 0.318757 tig0021657:+73482 Msa1393330:intron
GCCTTCATGGGCTGCGCTCC+CGG 0.320849 tig0021657:+73054 Msa1393330:CDS
AGCAAGCATTCTCCTGTTAT+GGG 0.321276 tig0021657:+73141 Msa1393330:CDS
AAGACGGTTTGGATATGTTT+TGG 0.323944 tig0021657:-70448 None:intergenic
TATCCTGATGAAATTCAATA+TGG 0.325981 tig0021657:+72357 Msa1393330:CDS
GGACTTCAAGGACCTTTCTA+TGG 0.332996 tig0021657:+72291 Msa1393330:CDS
TTCTGGTGAATCTTCACTAT+TGG 0.337945 tig0021657:-71269 None:intergenic
TTATTGTTGGCTTACCAATT+GGG 0.340053 tig0021657:+73203 Msa1393330:CDS
GGGCTGGCTTGTCTTATTGT+TGG 0.343431 tig0021657:+73190 Msa1393330:CDS
CAGTCTACGTTCAACATAAA+AGG 0.353647 tig0021657:-71998 None:intergenic
ACCGGAGCCAACCGCCTTCA+TGG 0.356349 tig0021657:+73041 Msa1393330:CDS
TCTGATTGTGGATATGAATA+TGG 0.372822 tig0021657:+72623 Msa1393330:CDS
TACCAATTGGGGTCTGCGTT+CGG 0.380901 tig0021657:+73215 Msa1393330:CDS
CACCAGAATTTAAACAAGAA+TGG 0.390854 tig0021657:+71284 Msa1393330:CDS
CCCATGAAGGCGGTTGGCTC+CGG 0.391178 tig0021657:-73042 None:intergenic
AATCTTGGTTTACTTATACT+TGG 0.395636 tig0021657:+70388 Msa1393330:CDS
CCAAAGAGGGTACCCATAAC+AGG 0.401351 tig0021657:-73153 None:intergenic
CATAGCATGTTGAATAATCT+TGG 0.402560 tig0021657:-72041 None:intergenic
TGGCACTTGTTGGGGTAAAC+AGG 0.403166 tig0021657:+72643 Msa1393330:CDS
CTTCCATATTGAATTTCATC+AGG 0.404640 tig0021657:-72360 None:intergenic
TATTCCAGGGACTTGGGCTA+TGG 0.404837 tig0021657:+73489 Msa1393330:intron
CATAGAAGAAATGCTATATA+TGG 0.408572 tig0021657:+72330 Msa1393330:CDS
CATATCCACAATCAGATACT+TGG 0.409548 tig0021657:-72616 None:intergenic
GTTAGTACCTTAACTATACT+TGG 0.411339 tig0021657:-71524 None:intergenic
TTCAACTTCATTCCAATTTG+AGG 0.413057 tig0021657:+71460 Msa1393330:CDS
TGTAAATTGCACAAGCAAAA+TGG 0.419368 tig0021657:-73798 None:intergenic
TCTAACCTTAGGAAAGATAC+TGG 0.419948 tig0021657:-73289 None:intergenic
GAAATTCAATATGGAAGAAA+AGG 0.424433 tig0021657:+72366 Msa1393330:CDS
TTTATGCAGTACATAATAAA+GGG 0.424631 tig0021657:+72261 Msa1393330:intron
AAGAAGTGCTGACTTGAAAA+TGG 0.427224 tig0021657:-73978 None:intergenic
GACCGAACGCAGACCCCAAT+TGG 0.428493 tig0021657:-73217 None:intergenic
CAACAAAAGAGATGGTCTTC+AGG 0.429438 tig0021657:+73099 Msa1393330:CDS
TCAACTTCATTCCAATTTGA+GGG 0.436853 tig0021657:+71461 Msa1393330:CDS
GACTTGAAAATGGTAGATAA+AGG 0.442931 tig0021657:-73968 None:intergenic
TACCGTTGAAGAAGACGGTT+TGG 0.445224 tig0021657:-70459 None:intergenic
GTGGCATGATTAAGCTCTTA+GGG 0.448921 tig0021657:+73654 Msa1393330:CDS
TTGTAATTGTGTTCATACTT+TGG 0.449990 tig0021657:-71834 None:intergenic
GGGAGTGATAATTCAATTGA+TGG 0.450914 tig0021657:+72560 Msa1393330:CDS
TCAATGGGTGGCTTCGTTTG+AGG 0.450979 tig0021657:+72159 Msa1393330:CDS
GATTGCTTTATCAAGAAGAA+TGG 0.454022 tig0021657:-72594 None:intergenic
CAATAAGATCAACAAGTGTA+TGG 0.456139 tig0021657:+73615 Msa1393330:CDS
GTTGTGCCAACCACAAAAGC+TGG 0.457175 tig0021657:-73776 None:intergenic
AAATCATCCAAGTATAGTTA+AGG 0.459304 tig0021657:+71517 Msa1393330:CDS
AAGGGCATGGGAGGACTTCA+AGG 0.461364 tig0021657:+72279 Msa1393330:CDS
TTTATTTACTATTACTCCAT+TGG 0.461466 tig0021657:+74121 Msa1393330:three_prime_UTR
GGTAACAGCAGGACTCCATT+TGG 0.463280 tig0021657:-70427 None:intergenic
AACAATGATGGTCTTTCTGA+TGG 0.466564 tig0021657:+71774 Msa1393330:CDS
CATTGTAGTAAGTAATCCTC+CGG 0.471086 tig0021657:-73073 None:intergenic
TTGTGGTTCTCAACACCAAA+TGG 0.475645 tig0021657:+70412 Msa1393330:CDS
CCGGAGCCAACCGCCTTCAT+GGG 0.476243 tig0021657:+73042 Msa1393330:CDS
GGTAAAATCCAATTTAGGGC+TGG 0.479956 tig0021657:+73174 Msa1393330:CDS
GCCACCCATTGATTTCCAAA+AGG 0.480343 tig0021657:-72148 None:intergenic
CTACAAGAATATCTATCAAA+AGG 0.480458 tig0021657:+73551 Msa1393330:CDS
GATATGAATATGGCACTTGT+TGG 0.481660 tig0021657:+72633 Msa1393330:CDS
CTTTGCATGTTCAATCCAGT+TGG 0.483865 tig0021657:-72991 None:intergenic
TTTGAAAGGGTTGTTTGCTA+GGG 0.488493 tig0021657:+73931 Msa1393330:CDS
CAAAAGGAAAGTCCAACTTC+TGG 0.490074 tig0021657:+73704 Msa1393330:CDS
GTTTACTTATACTTGGATTG+TGG 0.491342 tig0021657:+70395 Msa1393330:CDS
TAACAAGCTAGCTTATTAGC+TGG 0.493726 tig0021657:-71109 None:intergenic
GCTTTCTATCATCTGCAGAA+TGG 0.494608 tig0021657:+70194 Msa1393330:exon
CCACCCATTGATTTCCAAAA+GGG 0.496575 tig0021657:-72147 None:intergenic
AATCTTTGAAGTAACACAAA+AGG 0.498554 tig0021657:+73688 Msa1393330:CDS
TTTGGAAACTTCAGATGGGC+TGG 0.501126 tig0021657:+72941 Msa1393330:intron
ATATCAGAGGATGTACCAAC+TGG 0.502157 tig0021657:+72976 Msa1393330:CDS
CTGTCTTATTCCAGGGACTT+GGG 0.505447 tig0021657:+73483 Msa1393330:intron
TATTGTTGGCTTACCAATTG+GGG 0.508021 tig0021657:+73204 Msa1393330:CDS
TGCAATTAGCAGCTCATAGT+GGG 0.512001 tig0021657:+73846 Msa1393330:CDS
ATATGAATATGGCACTTGTT+GGG 0.515313 tig0021657:+72634 Msa1393330:CDS
GTGTTTGTTCCTCCATAGAA+AGG 0.515589 tig0021657:-72303 None:intergenic
TCTTCAGCAGCACCAGAAGT+TGG 0.518813 tig0021657:-73716 None:intergenic
AATTCGTAGACAAGAGTCTC+AGG 0.520861 tig0021657:+71959 Msa1393330:intron
GCAGTACATAATAAAGGGCA+TGG 0.521471 tig0021657:+72266 Msa1393330:intron
ATATTATACTTCTTACAGAA+AGG 0.524444 tig0021657:-71208 None:intergenic
TGATTATGCAATATGCAACT+AGG 0.530445 tig0021657:-73260 None:intergenic
TTAGCAGCTCATAGTGGGAA+TGG 0.534161 tig0021657:+73851 Msa1393330:CDS
GCGCAGCCCATGAAGGCGGT+TGG 0.535181 tig0021657:-73048 None:intergenic
GGATTGAACATGCAAAGAAA+AGG 0.537380 tig0021657:+72997 Msa1393330:CDS
TATAGCATTTCTTCTATGGA+AGG 0.538288 tig0021657:-72325 None:intergenic
TGAACATGCAAAGAAAAGGT+TGG 0.538850 tig0021657:+73001 Msa1393330:CDS
ATGATTGGTGGTTCAAGTAC+AGG 0.542267 tig0021657:-71046 None:intergenic
TGCTGATGGAGAAGAAAATA+CGG 0.543278 tig0021657:-70323 None:intergenic
TATGAACACAATTACAAAGC+AGG 0.544311 tig0021657:+71840 Msa1393330:CDS
CAACACCAGTATCTTTCCTA+AGG 0.548745 tig0021657:+73284 Msa1393330:CDS
ACAAAACCTTCACACTTCCA+TGG 0.550662 tig0021657:+70346 Msa1393330:CDS
TCCGGGAGCGCAGCCCATGA+AGG 0.551643 tig0021657:-73055 None:intergenic
ACAGTGTTCAATGTGATGAT+TGG 0.552842 tig0021657:-71061 None:intergenic
GTGCAATTAGCAGCTCATAG+TGG 0.552846 tig0021657:+73845 Msa1393330:CDS
TTGAAAGGGTTGTTTGCTAG+GGG 0.552992 tig0021657:+73932 Msa1393330:CDS
GTTATATTGGAGAACAATGA+TGG 0.553974 tig0021657:+71762 Msa1393330:CDS
TGTTTGCTAGGGGAAAATAT+GGG 0.554783 tig0021657:+73942 Msa1393330:CDS
ACACGCGGAAGCTCATTCTC+AGG 0.559771 tig0021657:-70998 None:intergenic
AGTGGGAATGGATGTGAACT+AGG 0.561160 tig0021657:+73863 Msa1393330:CDS
ATCCAAACCGTCTTCTTCAA+CGG 0.576571 tig0021657:+70457 Msa1393330:CDS
CATGAGAAAGCAAGAAACCA+TGG 0.578989 tig0021657:-70363 None:intergenic
TGAGAATGAGCTTCCGCGTG+TGG 0.580303 tig0021657:+71000 Msa1393330:CDS
TTGTGCCAACCACAAAAGCT+GGG 0.580530 tig0021657:-73775 None:intergenic
GCAATCCAAGTATCTGATTG+TGG 0.581850 tig0021657:+72611 Msa1393330:CDS
GTGTTCAATGTGATGATTGG+TGG 0.582750 tig0021657:-71058 None:intergenic
TACATAATAAAGGGCATGGG+AGG 0.589666 tig0021657:+72270 Msa1393330:CDS
GTTCAACATAAAAGGCGCAT+TGG 0.592570 tig0021657:-71990 None:intergenic
GCTTTATCAAGAAGAATGGA+AGG 0.597289 tig0021657:-72590 None:intergenic
GCTGAAGATGATGCAAATGC+TGG 0.597342 tig0021657:+73731 Msa1393330:CDS
GATTATGCAATATGCAACTA+GGG 0.597641 tig0021657:-73259 None:intergenic
CTAGATATGTAGACTAAGTG+AGG 0.598571 tig0021657:-71801 None:intergenic
AGCAGGAGCTATGAATGTGT+TGG 0.601659 tig0021657:+71857 Msa1393330:CDS
ATAAAAGGATTAAAATCAAG+AGG 0.605462 tig0021657:-70284 None:intergenic
TTACATACCGTTGAAGAAGA+CGG 0.605834 tig0021657:-70464 None:intergenic
CTTAGGAAAGATACTGGTGT+TGG 0.607492 tig0021657:-73283 None:intergenic
CAGTACATAATAAAGGGCAT+GGG 0.608875 tig0021657:+72267 Msa1393330:intron
CAATGATCCAACAAAAGAGA+TGG 0.612047 tig0021657:+73091 Msa1393330:CDS
CTTCAAGGACCTTTCTATGG+AGG 0.612281 tig0021657:+72294 Msa1393330:CDS
TATGAATATGGCACTTGTTG+GGG 0.620443 tig0021657:+72635 Msa1393330:CDS
AAGAAACCATGGAAGTGTGA+AGG 0.621466 tig0021657:-70352 None:intergenic
ATTGTAGTAAGTAATCCTCC+GGG 0.635850 tig0021657:-73072 None:intergenic
CCAATTTGAGGGAGAATATG+CGG 0.638104 tig0021657:+71472 Msa1393330:CDS
GGGAGCGCAGCCCATGAAGG+CGG 0.649683 tig0021657:-73052 None:intergenic
GTTATATGGATCAATATCAG+AGG 0.652685 tig0021657:+72963 Msa1393330:CDS
TTCATGGGCTGCGCTCCCGG+AGG 0.657126 tig0021657:+73057 Msa1393330:CDS
GAGATCAGAATGTTGTACAC+CGG 0.663139 tig0021657:+73023 Msa1393330:CDS
AAGATGATGCAAATGCTGGT+AGG 0.683909 tig0021657:+73735 Msa1393330:CDS
TGTGCCAACCACAAAAGCTG+GGG 0.694857 tig0021657:-73774 None:intergenic
GTCACAAACAAATCCACACG+CGG 0.716627 tig0021657:-71013 None:intergenic
TGCTAAAGAAGACAACTGTG+AGG 0.762382 tig0021657:-73122 None:intergenic
GTTGAACGTAGACTGTGACA+TGG 0.762585 tig0021657:+72006 Msa1393330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTCTAATTTTATTTTTG+TGG + tig0021657:71910-71929 Msa1393330:intron 10.0%
!!! AATTCTAATTTTATTTTTGT+GGG + tig0021657:71911-71930 Msa1393330:intron 10.0%
!! TAAATAACACTTGAAAATAA+TGG + tig0021657:70752-70771 Msa1393330:intron 15.0%
!! AGTTAATTAACAAAGTTTAA+TGG - tig0021657:72913-72932 None:intergenic 15.0%
!!! TAGTAAATAAAAAGTACATA+AGG - tig0021657:74112-74131 None:intergenic 15.0%
!! ATAAAAGGATTAAAATCAAG+AGG - tig0021657:70287-70306 None:intergenic 20.0%
!! AACAAAGAAGAGAAAATAAA+AGG - tig0021657:70302-70321 None:intergenic 20.0%
!!! TATTTTCAAGTGTTATTTAG+TGG - tig0021657:70751-70770 None:intergenic 20.0%
!! TACATTCAATAGTTAGTTAA+TGG - tig0021657:70849-70868 None:intergenic 20.0%
!! ATATTATACTTCTTACAGAA+AGG - tig0021657:71211-71230 None:intergenic 20.0%
!! ACTTCAGAAAAATATCTAAA+AGG - tig0021657:71247-71266 None:intergenic 20.0%
!!! TTATGACATAAGAAGTATAA+TGG + tig0021657:71367-71386 Msa1393330:intron 20.0%
!!! TTAGATATGTCTAGAAATTT+TGG - tig0021657:71657-71676 None:intergenic 20.0%
!!! ATTTTGATGGATTCTAAAAA+TGG + tig0021657:72067-72086 Msa1393330:CDS 20.0%
!!! TGTTTTCAACAATTTTATGA+TGG + tig0021657:72112-72131 Msa1393330:CDS 20.0%
!! TATAAACACTAAATTGTAAG+AGG - tig0021657:72219-72238 None:intergenic 20.0%
!! TTTATGCAGTACATAATAAA+GGG + tig0021657:72261-72280 Msa1393330:intron 20.0%
!!! GAGTTTTTTTAGTATCATAT+AGG - tig0021657:72439-72458 None:intergenic 20.0%
!!! CACTATTTTGTATCTTTATT+AGG + tig0021657:72458-72477 Msa1393330:intron 20.0%
!! AAGATATTAATACAGCAATT+TGG + tig0021657:72494-72513 Msa1393330:CDS 20.0%
!!! TATTTTTTGTTAATGAGAAC+AGG + tig0021657:72714-72733 Msa1393330:intron 20.0%
!! AACTTTGTTAATTAACTTTG+AGG + tig0021657:72916-72935 Msa1393330:intron 20.0%
!!! TTAATTAACTTTGAGGATTT+TGG + tig0021657:72923-72942 Msa1393330:intron 20.0%
!!! CAAGTTGAATTAAATATTGA+GGG + tig0021657:73335-73354 Msa1393330:intron 20.0%
!!! AAGGATTATCATTAAGATTT+TGG + tig0021657:73570-73589 Msa1393330:CDS 20.0%
!! TTTATTTACTATTACTCCAT+TGG + tig0021657:74121-74140 Msa1393330:three_prime_UTR 20.0%
! TTCCTCTCTATGAAAAAATT+TGG + tig0021657:70232-70251 Msa1393330:CDS 25.0%
!!! ATCCAAATTTTTTCATAGAG+AGG - tig0021657:70237-70256 None:intergenic 25.0%
! AATCTTGGTTTACTTATACT+TGG + tig0021657:70388-70407 Msa1393330:CDS 25.0%
! TATGTAGTTAAGATGCTTAA+TGG + tig0021657:70699-70718 Msa1393330:intron 25.0%
!!! TGTTCTGTTTTTACTTTCTA+TGG + tig0021657:71148-71167 Msa1393330:CDS 25.0%
!! TTTTACTTTCTATGGTCTTT+TGG + tig0021657:71156-71175 Msa1393330:CDS 25.0%
!!! TTCCAAATTTGCTAAGTTTT+GGG + tig0021657:71183-71202 Msa1393330:CDS 25.0%
!! AACCATTCTTGTTTAAATTC+TGG - tig0021657:71289-71308 None:intergenic 25.0%
! ACAAGAATGGTTAAAAATGA+AGG + tig0021657:71297-71316 Msa1393330:CDS 25.0%
! AAATCATCCAAGTATAGTTA+AGG + tig0021657:71517-71536 Msa1393330:CDS 25.0%
!!! TACATGTAATCCTTGTTTTT+CGG + tig0021657:71581-71600 Msa1393330:intron 25.0%
! TTGTAATTGTGTTCATACTT+TGG - tig0021657:71837-71856 None:intergenic 25.0%
! CTTTATGCAGTACATAATAA+AGG + tig0021657:72260-72279 Msa1393330:intron 25.0%
! CATATATAGCATTTCTTCTA+TGG - tig0021657:72332-72351 None:intergenic 25.0%
! CATAGAAGAAATGCTATATA+TGG + tig0021657:72330-72349 Msa1393330:CDS 25.0%
! TATCCTGATGAAATTCAATA+TGG + tig0021657:72357-72376 Msa1393330:CDS 25.0%
! GAAATTCAATATGGAAGAAA+AGG + tig0021657:72366-72385 Msa1393330:CDS 25.0%
! AATAGTAACTGACTAAGATA+TGG - tig0021657:72405-72424 None:intergenic 25.0%
! TAAATTGAAGTCAATAGAGT+TGG + tig0021657:72818-72837 Msa1393330:intron 25.0%
!! AAATTGGATTTTACCAAAGA+GGG - tig0021657:73169-73188 None:intergenic 25.0%
!! TAAATTGGATTTTACCAAAG+AGG - tig0021657:73170-73189 None:intergenic 25.0%
! TCTTTGGTAAAATCCAATTT+AGG + tig0021657:73169-73188 Msa1393330:CDS 25.0%
! CTTTGGTAAAATCCAATTTA+GGG + tig0021657:73170-73189 Msa1393330:CDS 25.0%
!! GCAAGTTGAATTAAATATTG+AGG + tig0021657:73334-73353 Msa1393330:intron 25.0%
! GACTATTTGTCAGATATAAT+TGG + tig0021657:73408-73427 Msa1393330:intron 25.0%
! TAGATCACAAAAAGAGTTAA+AGG - tig0021657:73518-73537 None:intergenic 25.0%
! CTACAAGAATATCTATCAAA+AGG + tig0021657:73551-73570 Msa1393330:CDS 25.0%
!! GATTATCATTAAGATTTTGG+TGG + tig0021657:73573-73592 Msa1393330:CDS 25.0%
!!! AATCTTTGAAGTAACACAAA+AGG + tig0021657:73688-73707 Msa1393330:CDS 25.0%
! TGTATCTAGTAGTATGTTAT+TGG + tig0021657:73903-73922 Msa1393330:CDS 25.0%
!!! AGTATTAGTCTTGAGATTAA+TGG + tig0021657:74050-74069 Msa1393330:three_prime_UTR 25.0%
!!! TACACTTATTTTAAGACCAA+TGG - tig0021657:74140-74159 None:intergenic 25.0%
GTTTACTTATACTTGGATTG+TGG + tig0021657:70395-70414 Msa1393330:CDS 30.0%
! AGATGCTTAATGGATATATG+AGG + tig0021657:70709-70728 Msa1393330:intron 30.0%
!! GTTTGCGAAAACTTTTTAAG+TGG - tig0021657:70909-70928 None:intergenic 30.0%
!!! CTTCCAAATTTGCTAAGTTT+TGG + tig0021657:71182-71201 Msa1393330:CDS 30.0%
TACCCAAAACTTAGCAAATT+TGG - tig0021657:71188-71207 None:intergenic 30.0%
CACCAGAATTTAAACAAGAA+TGG + tig0021657:71284-71303 Msa1393330:CDS 30.0%
CATGTATATCAATCATTGCA+GGG - tig0021657:71340-71359 None:intergenic 30.0%
ACATGTATATCAATCATTGC+AGG - tig0021657:71341-71360 None:intergenic 30.0%
TTCAACTTCATTCCAATTTG+AGG + tig0021657:71460-71479 Msa1393330:CDS 30.0%
TCAACTTCATTCCAATTTGA+GGG + tig0021657:71461-71480 Msa1393330:CDS 30.0%
GTTAGTACCTTAACTATACT+TGG - tig0021657:71527-71546 None:intergenic 30.0%
!!! ACATGTAATCCTTGTTTTTC+GGG + tig0021657:71582-71601 Msa1393330:intron 30.0%
!! TAGAAATTTTGGTGAAACTG+CGG - tig0021657:71646-71665 None:intergenic 30.0%
!!! ACGTGTAATGTTAGTCTTTT+AGG - tig0021657:71736-71755 None:intergenic 30.0%
AAAAGACTAACATTACACGT+TGG + tig0021657:71736-71755 Msa1393330:intron 30.0%
GTTATATTGGAGAACAATGA+TGG + tig0021657:71762-71781 Msa1393330:CDS 30.0%
TATGAACACAATTACAAAGC+AGG + tig0021657:71840-71859 Msa1393330:CDS 30.0%
TCAATTGAGAAAGATAAGCA+TGG - tig0021657:71939-71958 None:intergenic 30.0%
CATAGCATGTTGAATAATCT+TGG - tig0021657:72044-72063 None:intergenic 30.0%
TATAGCATTTCTTCTATGGA+AGG - tig0021657:72328-72347 None:intergenic 30.0%
CTTCCATATTGAATTTCATC+AGG - tig0021657:72363-72382 None:intergenic 30.0%
GATTGCTTTATCAAGAAGAA+TGG - tig0021657:72597-72616 None:intergenic 30.0%
TCTGATTGTGGATATGAATA+TGG + tig0021657:72623-72642 Msa1393330:CDS 30.0%
ATATGAATATGGCACTTGTT+GGG + tig0021657:72634-72653 Msa1393330:CDS 30.0%
GCAAAATGTTTGATTCTCAT+TGG - tig0021657:72789-72808 None:intergenic 30.0%
! AGTTTTACACCGACATTTAA+CGG - tig0021657:72868-72887 None:intergenic 30.0%
GTTATATGGATCAATATCAG+AGG + tig0021657:72963-72982 Msa1393330:CDS 30.0%
!! TTATTGTTGGCTTACCAATT+GGG + tig0021657:73203-73222 Msa1393330:CDS 30.0%
GATTATGCAATATGCAACTA+GGG - tig0021657:73262-73281 None:intergenic 30.0%
TGATTATGCAATATGCAACT+AGG - tig0021657:73263-73282 None:intergenic 30.0%
TAATGCGAAAATCTAACCTT+AGG - tig0021657:73303-73322 None:intergenic 30.0%
!!! GGTTTTTTGCATTAGTCTAT+TGG + tig0021657:73356-73375 Msa1393330:intron 30.0%
CAATAAGATCAACAAGTGTA+TGG + tig0021657:73615-73634 Msa1393330:CDS 30.0%
TCAACAAGTGTATGGTTTAT+TGG + tig0021657:73623-73642 Msa1393330:CDS 30.0%
!! TGGTTTATTGGATTTTTGAG+TGG + tig0021657:73635-73654 Msa1393330:CDS 30.0%
TGTAAATTGCACAAGCAAAA+TGG - tig0021657:73801-73820 None:intergenic 30.0%
!!! AGCACTTGTTGTTAAGATTT+TGG + tig0021657:73820-73839 Msa1393330:CDS 30.0%
!!! GCACTTGTTGTTAAGATTTT+GGG + tig0021657:73821-73840 Msa1393330:CDS 30.0%
!!! CACTTGTTGTTAAGATTTTG+GGG + tig0021657:73822-73841 Msa1393330:CDS 30.0%
!!! ACTTGTTGTTAAGATTTTGG+GGG + tig0021657:73823-73842 Msa1393330:CDS 30.0%
!!! TGTTATTGGCCATTTTTGAA+AGG + tig0021657:73917-73936 Msa1393330:CDS 30.0%
!!! GTTATTGGCCATTTTTGAAA+GGG + tig0021657:73918-73937 Msa1393330:CDS 30.0%
GACTTGAAAATGGTAGATAA+AGG - tig0021657:73971-73990 None:intergenic 30.0%
! CATAGAGAGGAAGATTTTCT+TGG - tig0021657:70224-70243 None:intergenic 35.0%
TGCTGATGGAGAAGAAAATA+CGG - tig0021657:70326-70345 None:intergenic 35.0%
TTGCTTTCTCATGTGAATCT+TGG + tig0021657:70373-70392 Msa1393330:CDS 35.0%
!! AAGACGGTTTGGATATGTTT+TGG - tig0021657:70451-70470 None:intergenic 35.0%
TTACATACCGTTGAAGAAGA+CGG - tig0021657:70467-70486 None:intergenic 35.0%
ATTAACTACGAACTCGAAAG+TGG - tig0021657:70781-70800 None:intergenic 35.0%
!!! GCGAAAACTTTTTAAGTGGT+TGG - tig0021657:70905-70924 None:intergenic 35.0%
! TCTTGCTAATTAGTCTCTCA+AGG + tig0021657:70935-70954 Msa1393330:intron 35.0%
ACAGTGTTCAATGTGATGAT+TGG - tig0021657:71064-71083 None:intergenic 35.0%
! TAACAAGCTAGCTTATTAGC+TGG - tig0021657:71112-71131 None:intergenic 35.0%
!! GCTTGTTATGTTTCTGATGA+TGG + tig0021657:71124-71143 Msa1393330:CDS 35.0%
TTCTGGTGAATCTTCACTAT+TGG - tig0021657:71272-71291 None:intergenic 35.0%
! TTGTCTTTTGTGATTGTTGC+AGG + tig0021657:71391-71410 Msa1393330:intron 35.0%
!!! CATGTAATCCTTGTTTTTCG+GGG + tig0021657:71583-71602 Msa1393330:intron 35.0%
AACAATGATGGTCTTTCTGA+TGG + tig0021657:71774-71793 Msa1393330:CDS 35.0%
CTAGATATGTAGACTAAGTG+AGG - tig0021657:71804-71823 None:intergenic 35.0%
CAGTCTACGTTCAACATAAA+AGG - tig0021657:72001-72020 None:intergenic 35.0%
! ACATGCTATGTGCATTTTGA+TGG + tig0021657:72054-72073 Msa1393330:CDS 35.0%
! GGAATTAAAGATGACCCTTT+TGG + tig0021657:72133-72152 Msa1393330:CDS 35.0%
!! ATGACCCTTTTGGAAATCAA+TGG + tig0021657:72143-72162 Msa1393330:CDS 35.0%
!! TGACCCTTTTGGAAATCAAT+GGG + tig0021657:72144-72163 Msa1393330:CDS 35.0%
! CAGTACATAATAAAGGGCAT+GGG + tig0021657:72267-72286 Msa1393330:intron 35.0%
! TCAGTAACACATGCTTTTGA+AGG + tig0021657:72539-72558 Msa1393330:CDS 35.0%
! CAGTAACACATGCTTTTGAA+GGG + tig0021657:72540-72559 Msa1393330:CDS 35.0%
GGGAGTGATAATTCAATTGA+TGG + tig0021657:72560-72579 Msa1393330:CDS 35.0%
GCTTTATCAAGAAGAATGGA+AGG - tig0021657:72593-72612 None:intergenic 35.0%
CATATCCACAATCAGATACT+TGG - tig0021657:72619-72638 None:intergenic 35.0%
GATATGAATATGGCACTTGT+TGG + tig0021657:72633-72652 Msa1393330:CDS 35.0%
! TATGAATATGGCACTTGTTG+GGG + tig0021657:72635-72654 Msa1393330:CDS 35.0%
TTGATTCTCATTGGACTCAT+TGG - tig0021657:72780-72799 None:intergenic 35.0%
! GTTTTACACCGACATTTAAC+GGG - tig0021657:72867-72886 None:intergenic 35.0%
!! AGGATTTTGGAAACTTCAGA+TGG + tig0021657:72936-72955 Msa1393330:intron 35.0%
!! GGATTTTGGAAACTTCAGAT+GGG + tig0021657:72937-72956 Msa1393330:intron 35.0%
GGATTGAACATGCAAAGAAA+AGG + tig0021657:72997-73016 Msa1393330:CDS 35.0%
TGAACATGCAAAGAAAAGGT+TGG + tig0021657:73001-73020 Msa1393330:CDS 35.0%
ATTGTAGTAAGTAATCCTCC+GGG - tig0021657:73075-73094 None:intergenic 35.0%
CATTGTAGTAAGTAATCCTC+CGG - tig0021657:73076-73095 None:intergenic 35.0%
CAATGATCCAACAAAAGAGA+TGG + tig0021657:73091-73110 Msa1393330:CDS 35.0%
!! CTTATTGTTGGCTTACCAAT+TGG + tig0021657:73202-73221 Msa1393330:CDS 35.0%
!! TATTGTTGGCTTACCAATTG+GGG + tig0021657:73204-73223 Msa1393330:CDS 35.0%
TCTAACCTTAGGAAAGATAC+TGG - tig0021657:73292-73311 None:intergenic 35.0%
AAGTACGTATGCTAGTAGAT+AGG - tig0021657:73384-73403 None:intergenic 35.0%
GCAAACAACCCTTTCAAAAA+TGG - tig0021657:73929-73948 None:intergenic 35.0%
!! TTTTGAAAGGGTTGTTTGCT+AGG + tig0021657:73930-73949 Msa1393330:CDS 35.0%
! TTTGAAAGGGTTGTTTGCTA+GGG + tig0021657:73931-73950 Msa1393330:CDS 35.0%
! TTGTTTGCTAGGGGAAAATA+TGG + tig0021657:73941-73960 Msa1393330:CDS 35.0%
! TGTTTGCTAGGGGAAAATAT+GGG + tig0021657:73942-73961 Msa1393330:CDS 35.0%
!! AAGAAGTGCTGACTTGAAAA+TGG - tig0021657:73981-74000 None:intergenic 35.0%
GCTTTCTATCATCTGCAGAA+TGG + tig0021657:70194-70213 Msa1393330:exon 40.0%
!!! TGTGAAGGTTTTGTTGCTGA+TGG - tig0021657:70340-70359 None:intergenic 40.0%
ACAAAACCTTCACACTTCCA+TGG + tig0021657:70346-70365 Msa1393330:CDS 40.0%
! AAGAAACCATGGAAGTGTGA+AGG - tig0021657:70355-70374 None:intergenic 40.0%
CATGAGAAAGCAAGAAACCA+TGG - tig0021657:70366-70385 None:intergenic 40.0%
TTGTGGTTCTCAACACCAAA+TGG + tig0021657:70412-70431 Msa1393330:CDS 40.0%
!!! GGATATGTTTTGGTAACAGC+AGG - tig0021657:70441-70460 None:intergenic 40.0%
ATCCAAACCGTCTTCTTCAA+CGG + tig0021657:70457-70476 Msa1393330:CDS 40.0%
GAAATCAGTGCAAGACTTCA+TGG - tig0021657:70650-70669 None:intergenic 40.0%
TCATTCTCAGGTACTCTAGT+AGG - tig0021657:70989-71008 None:intergenic 40.0%
! ATGATTGGTGGTTCAAGTAC+AGG - tig0021657:71049-71068 None:intergenic 40.0%
GTGTTCAATGTGATGATTGG+TGG - tig0021657:71061-71080 None:intergenic 40.0%
! GAACACTGTTTTGTCTCTGT+TGG + tig0021657:71075-71094 Msa1393330:CDS 40.0%
! GCGTCACATCTTCAATTTTG+TGG - tig0021657:71436-71455 None:intergenic 40.0%
CCAATTTGAGGGAGAATATG+CGG + tig0021657:71472-71491 Msa1393330:CDS 40.0%
CGAATTCAAGCATGAGTACT+TGG - tig0021657:71707-71726 None:intergenic 40.0%
TACACGTTGGCAGGTTATAT+TGG + tig0021657:71749-71768 Msa1393330:intron 40.0%
AATTCGTAGACAAGAGTCTC+AGG + tig0021657:71959-71978 Msa1393330:intron 40.0%
! GTTCAACATAAAAGGCGCAT+TGG - tig0021657:71993-72012 None:intergenic 40.0%
!! CCACCCATTGATTTCCAAAA+GGG - tig0021657:72150-72169 None:intergenic 40.0%
GCAGTACATAATAAAGGGCA+TGG + tig0021657:72266-72285 Msa1393330:intron 40.0%
! TACATAATAAAGGGCATGGG+AGG + tig0021657:72270-72289 Msa1393330:CDS 40.0%
GTGTTTGTTCCTCCATAGAA+AGG - tig0021657:72306-72325 None:intergenic 40.0%
GCAATCCAAGTATCTGATTG+TGG + tig0021657:72611-72630 Msa1393330:CDS 40.0%
CATGAACTCCCGTTAAATGT+CGG + tig0021657:72856-72875 Msa1393330:intron 40.0%
ATATCAGAGGATGTACCAAC+TGG + tig0021657:72976-72995 Msa1393330:CDS 40.0%
CTTTGCATGTTCAATCCAGT+TGG - tig0021657:72994-73013 None:intergenic 40.0%
GAGATCAGAATGTTGTACAC+CGG + tig0021657:73023-73042 Msa1393330:CDS 40.0%
! CTGAAGACCATCTCTTTTGT+TGG - tig0021657:73101-73120 None:intergenic 40.0%
CAACAAAAGAGATGGTCTTC+AGG + tig0021657:73099-73118 Msa1393330:CDS 40.0%
TGCTAAAGAAGACAACTGTG+AGG - tig0021657:73125-73144 None:intergenic 40.0%
TAGCAAGCATTCTCCTGTTA+TGG + tig0021657:73140-73159 Msa1393330:CDS 40.0%
AGCAAGCATTCTCCTGTTAT+GGG + tig0021657:73141-73160 Msa1393330:CDS 40.0%
! GGTAAAATCCAATTTAGGGC+TGG + tig0021657:73174-73193 Msa1393330:CDS 40.0%
CTTAGGAAAGATACTGGTGT+TGG - tig0021657:73286-73305 None:intergenic 40.0%
CAACACCAGTATCTTTCCTA+AGG + tig0021657:73284-73303 Msa1393330:CDS 40.0%
AGTGGCATGATTAAGCTCTT+AGG + tig0021657:73653-73672 Msa1393330:CDS 40.0%
GTGGCATGATTAAGCTCTTA+GGG + tig0021657:73654-73673 Msa1393330:CDS 40.0%
CAAAAGGAAAGTCCAACTTC+TGG + tig0021657:73704-73723 Msa1393330:CDS 40.0%
AAGATGATGCAAATGCTGGT+AGG + tig0021657:73735-73754 Msa1393330:CDS 40.0%
TGCAATTAGCAGCTCATAGT+GGG + tig0021657:73846-73865 Msa1393330:CDS 40.0%
! TTGAAAGGGTTGTTTGCTAG+GGG + tig0021657:73932-73951 Msa1393330:CDS 40.0%
TACCGTTGAAGAAGACGGTT+TGG - tig0021657:70462-70481 None:intergenic 45.0%
AGTTGCAGCGACATCAAGAT+CGG + tig0021657:70568-70587 Msa1393330:intron 45.0%
TGTTGCAGTTTATGCTGCAG+TGG + tig0021657:70609-70628 Msa1393330:intron 45.0%
GTCACAAACAAATCCACACG+CGG - tig0021657:71016-71035 None:intergenic 45.0%
CCGCATATTCTCCCTCAAAT+TGG - tig0021657:71475-71494 None:intergenic 45.0%
!!! GTAATCCTTGTTTTTCGGGG+AGG + tig0021657:71586-71605 Msa1393330:intron 45.0%
CGTTCATTCCTAGTCGATGT+AGG + tig0021657:71680-71699 Msa1393330:intron 45.0%
! TACTTGGTCCTACATCGACT+AGG - tig0021657:71691-71710 None:intergenic 45.0%
GACTAACATTACACGTTGGC+AGG + tig0021657:71740-71759 Msa1393330:intron 45.0%
AGCAGGAGCTATGAATGTGT+TGG + tig0021657:71857-71876 Msa1393330:CDS 45.0%
GTTGAACGTAGACTGTGACA+TGG + tig0021657:72006-72025 Msa1393330:CDS 45.0%
!! CCCTTTTGGAAATCAATGGG+TGG + tig0021657:72147-72166 Msa1393330:CDS 45.0%
!! GCCACCCATTGATTTCCAAA+AGG - tig0021657:72151-72170 None:intergenic 45.0%
GGACTTCAAGGACCTTTCTA+TGG + tig0021657:72291-72310 Msa1393330:CDS 45.0%
CTTCAAGGACCTTTCTATGG+AGG + tig0021657:72294-72313 Msa1393330:CDS 45.0%
TTTGGAAACTTCAGATGGGC+TGG + tig0021657:72941-72960 Msa1393330:intron 45.0%
!! CTTCAGATGGGCTGGTTATA+TGG + tig0021657:72949-72968 Msa1393330:intron 45.0%
AAGACAAGCCAGCCCTAAAT+TGG - tig0021657:73185-73204 None:intergenic 45.0%
! ACCTTTCGCTGTCTTATTCC+AGG + tig0021657:73475-73494 Msa1393330:intron 45.0%
! CCTTTCGCTGTCTTATTCCA+GGG + tig0021657:73476-73495 Msa1393330:intron 45.0%
! CTGTCTTATTCCAGGGACTT+GGG + tig0021657:73483-73502 Msa1393330:intron 45.0%
GCTGAAGATGATGCAAATGC+TGG + tig0021657:73731-73750 Msa1393330:CDS 45.0%
TTGTGCCAACCACAAAAGCT+GGG - tig0021657:73778-73797 None:intergenic 45.0%
GTGCAATTAGCAGCTCATAG+TGG + tig0021657:73845-73864 Msa1393330:CDS 45.0%
! TTAGCAGCTCATAGTGGGAA+TGG + tig0021657:73851-73870 Msa1393330:CDS 45.0%
AGTGGGAATGGATGTGAACT+AGG + tig0021657:73863-73882 Msa1393330:CDS 45.0%
GGTAACAGCAGGACTCCATT+TGG - tig0021657:70430-70449 None:intergenic 50.0%
TGCAGTTTATGCTGCAGTGG+CGG + tig0021657:70612-70631 Msa1393330:intron 50.0%
GTCAACCTCCCCGAAAAACA+AGG - tig0021657:71594-71613 None:intergenic 50.0%
TCAATGGGTGGCTTCGTTTG+AGG + tig0021657:72159-72178 Msa1393330:CDS 50.0%
! TGGCACTTGTTGGGGTAAAC+AGG + tig0021657:72643-72662 Msa1393330:CDS 50.0%
!! CCAAAGAGGGTACCCATAAC+AGG - tig0021657:73156-73175 None:intergenic 50.0%
CCTGTTATGGGTACCCTCTT+TGG + tig0021657:73153-73172 Msa1393330:CDS 50.0%
!! GGGCTGGCTTGTCTTATTGT+TGG + tig0021657:73190-73209 Msa1393330:CDS 50.0%
TACCAATTGGGGTCTGCGTT+CGG + tig0021657:73215-73234 Msa1393330:CDS 50.0%
CCCTGGAATAAGACAGCGAA+AGG - tig0021657:73479-73498 None:intergenic 50.0%
! GCTGTCTTATTCCAGGGACT+TGG + tig0021657:73482-73501 Msa1393330:intron 50.0%
TATTCCAGGGACTTGGGCTA+TGG + tig0021657:73489-73508 Msa1393330:intron 50.0%
TCTTCAGCAGCACCAGAAGT+TGG - tig0021657:73719-73738 None:intergenic 50.0%
! TGATGAGTCCCCAGCTTTTG+TGG + tig0021657:73766-73785 Msa1393330:CDS 50.0%
TGTGCCAACCACAAAAGCTG+GGG - tig0021657:73777-73796 None:intergenic 50.0%
GTTGTGCCAACCACAAAAGC+TGG - tig0021657:73779-73798 None:intergenic 50.0%
ACACGCGGAAGCTCATTCTC+AGG - tig0021657:71001-71020 None:intergenic 55.0%
TGAGAATGAGCTTCCGCGTG+TGG + tig0021657:71000-71019 Msa1393330:CDS 55.0%
GGTGTCCTGGTCTCTCTTGT+CGG - tig0021657:71625-71644 None:intergenic 55.0%
TTGGTGAAACTGCGGTGTCC+TGG - tig0021657:71638-71657 None:intergenic 55.0%
! AAGGGCATGGGAGGACTTCA+AGG + tig0021657:72279-72298 Msa1393330:CDS 55.0%
GAATCCATAGCCCAAGTCCC+TGG - tig0021657:73496-73515 None:intergenic 55.0%
! GAGTCCCCAGCTTTTGTGGT+TGG + tig0021657:73770-73789 Msa1393330:CDS 55.0%
CGTGACCGACAAGAGAGACC+AGG + tig0021657:71617-71636 Msa1393330:intron 60.0%
GACCGAACGCAGACCCCAAT+TGG - tig0021657:73220-73239 None:intergenic 60.0%
ACCGGAGCCAACCGCCTTCA+TGG + tig0021657:73041-73060 Msa1393330:CDS 65.0%
! CCCATGAAGGCGGTTGGCTC+CGG - tig0021657:73045-73064 None:intergenic 65.0%
CCGGAGCCAACCGCCTTCAT+GGG + tig0021657:73042-73061 Msa1393330:CDS 65.0%
GCGCAGCCCATGAAGGCGGT+TGG - tig0021657:73051-73070 None:intergenic 70.0%
! GGGAGCGCAGCCCATGAAGG+CGG - tig0021657:73055-73074 None:intergenic 70.0%
! GCCTTCATGGGCTGCGCTCC+CGG + tig0021657:73054-73073 Msa1393330:CDS 70.0%
! TCCGGGAGCGCAGCCCATGA+AGG - tig0021657:73058-73077 None:intergenic 70.0%
! TTCATGGGCTGCGCTCCCGG+AGG + tig0021657:73057-73076 Msa1393330:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0021657 gene 70181 74180 70181 ID=Msa1393330;Name=Msa1393330
tig0021657 mRNA 70181 74180 70181 ID=Msa1393330-mRNA-1;Parent=Msa1393330;Name=Msa1393330-mRNA-1;_AED=0.05;_eAED=0.05;_QI=32|1|1|1|1|1|9|135|749
tig0021657 exon 70181 70478 70181 ID=Msa1393330-mRNA-1:exon:13692;Parent=Msa1393330-mRNA-1
tig0021657 exon 70994 71318 70994 ID=Msa1393330-mRNA-1:exon:13693;Parent=Msa1393330-mRNA-1
tig0021657 exon 71413 71538 71413 ID=Msa1393330-mRNA-1:exon:13694;Parent=Msa1393330-mRNA-1
tig0021657 exon 71762 71878 71762 ID=Msa1393330-mRNA-1:exon:13695;Parent=Msa1393330-mRNA-1
tig0021657 exon 71968 72180 71968 ID=Msa1393330-mRNA-1:exon:13696;Parent=Msa1393330-mRNA-1
tig0021657 exon 72270 72387 72270 ID=Msa1393330-mRNA-1:exon:13697;Parent=Msa1393330-mRNA-1
tig0021657 exon 72480 72664 72480 ID=Msa1393330-mRNA-1:exon:13698;Parent=Msa1393330-mRNA-1
tig0021657 exon 72955 73305 72955 ID=Msa1393330-mRNA-1:exon:13699;Parent=Msa1393330-mRNA-1
tig0021657 exon 73497 74180 73497 ID=Msa1393330-mRNA-1:exon:13700;Parent=Msa1393330-mRNA-1
tig0021657 five_prime_UTR 70181 70212 70181 ID=Msa1393330-mRNA-1:five_prime_utr;Parent=Msa1393330-mRNA-1
tig0021657 CDS 70213 70478 70213 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 70994 71318 70994 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 71413 71538 71413 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 71762 71878 71762 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 71968 72180 71968 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 72270 72387 72270 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 72480 72664 72480 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 72955 73305 72955 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 CDS 73497 74045 73497 ID=Msa1393330-mRNA-1:cds;Parent=Msa1393330-mRNA-1
tig0021657 three_prime_UTR 74046 74180 74046 ID=Msa1393330-mRNA-1:three_prime_utr;Parent=Msa1393330-mRNA-1
Gene Sequence

>Msa1393330

ATGGTCAACCAAGAAAATCTTCCTCTCTATGAAAAAATTTGGATTAAACGCACACTTCAAAGAGCAATAGACCTCTTGATTTTAATCCTTTTATTTTCTCTTCTTTGTTACCGTATTTTCTTCTCCATCAGCAACAAAACCTTCACACTTCCATGGTTTCTTGCTTTCTCATGTGAATCTTGGTTTACTTATACTTGGATTGTGGTTCTCAACACCAAATGGAGTCCTGCTGTTACCAAAACATATCCAAACCGTCTTCTTCAACGAGTACCTGAGAATGAGCTTCCGCGTGTGGATTTGTTTGTGACAACAGCAGATCCTGTACTTGAACCACCAATCATCACATTGAACACTGTTTTGTCTCTGTTGGCTCTTGATTATCCAGCTAATAAGCTAGCTTGTTATGTTTCTGATGATGGCTGTTCTGTTTTTACTTTCTATGGTCTTTTGGAAGCTTCCAAATTTGCTAAGTTTTGGGTACCTTTCTGTAAGAAGTATAATATACAAGTTAGAGCACCTTTTAGATATTTTTCTGAAGTTACCAATAGTGAAGATTCACCAGAATTTAAACAAGAATGGTTAAAAATGAAGGATATGTATGACAATCTTAGCCACAAAATTGAAGATGTGACGCTAAATTCAACTTCATTCCAATTTGAGGGAGAATATGCGGTGTTCTTAAATACAGAAAAAAGAAATCATCCAAGTATAGTTAAGGTTATATTGGAGAACAATGATGGTCTTTCTGATGGATTGCCTCACTTAGTCTACATATCTAGAGAGAAGAGACCAAAGTATGAACACAATTACAAAGCAGGAGCTATGAATGTGTTGACAAGAGTCTCAGGATTGATGACCAATGCGCCTTTTATGTTGAACGTAGACTGTGACATGGTTGTGAACAATGCCAAGATTATTCAACATGCTATGTGCATTTTGATGGATTCTAAAAATGGAAAAGATGTTGCATTTGTTCAATGTTTTCAACAATTTTATGATGGAATTAAAGATGACCCTTTTGGAAATCAATGGGTGGCTTCGTTTGAGTACATAATAAAGGGCATGGGAGGACTTCAAGGACCTTTCTATGGAGGAACAAACACCTTCCATAGAAGAAATGCTATATATGGTCTTTATCCTGATGAAATTCAATATGGAAGAAAAGGAAAAATAACAGAAAAGATATTAATACAGCAATTTGGAAGCTCAAGAGAGTTTGTCAAATCAGTAACACATGCTTTTGAAGGGAGTGATAATTCAATTGATGGTATCAGTCCTTCCATTCTTCTTGATAAAGCAATCCAAGTATCTGATTGTGGATATGAATATGGCACTTGTTGGGGTAAACAGATGGGCTGGTTATATGGATCAATATCAGAGGATGTACCAACTGGATTGAACATGCAAAGAAAAGGTTGGAGATCAGAATGTTGTACACCGGAGCCAACCGCCTTCATGGGCTGCGCTCCCGGAGGATTACTTACTACAATGATCCAACAAAAGAGATGGTCTTCAGGCCTCACAGTTGTCTTCTTTAGCAAGCATTCTCCTGTTATGGGTACCCTCTTTGGTAAAATCCAATTTAGGGCTGGCTTGTCTTATTGTTGGCTTACCAATTGGGGTCTGCGTTCGGTCTTTGAAGTTTCTTATGCAGCCCTAGTTGCATATTGCATAATCACCAACACCAGTATCTTTCCTAAGGGACTTGGGCTATGGATTCCTTTAACTCTTTTTGTGATCTATACTATACATACACTACAAGAATATCTATCAAAAGGATTATCATTAAGATTTTGGTGGAACAATCAGAGAATGATTACAATAAGATCAACAAGTGTATGGTTTATTGGATTTTTGAGTGGCATGATTAAGCTCTTAGGGATATCTGATACAATCTTTGAAGTAACACAAAAGGAAAGTCCAACTTCTGGTGCTGCTGAAGATGATGCAAATGCTGGTAGGTTCACATTTGATGAGTCCCCAGCTTTTGTGGTTGGCACAACCATTTTGCTTGTGCAATTTACAGCACTTGTTGTTAAGATTTTGGGGGTGCAATTAGCAGCTCATAGTGGGAATGGATGTGAACTAGGTGAGTTAATGTGTAGTGTGTATCTAGTAGTATGTTATTGGCCATTTTTGAAAGGGTTGTTTGCTAGGGGAAAATATGGGATTCCTTTATCTACCATTTTCAAGTCAGCACTTCTTACATTTATTTTTGTGCACTTCAGTAGAATTTCTGTTGTAAGTTGA
Protein sequence

>Msa1393330

MVNQENLPLYEKIWIKRTLQRAIDLLILILLFSLLCYRIFFSISNKTFTLPWFLAFSCESWFTYTWIVVLNTKWSPAVTKTYPNRLLQRVPENELPRVDLFVTTADPVLEPPIITLNTVLSLLALDYPANKLACYVSDDGCSVFTFYGLLEASKFAKFWVPFCKKYNIQVRAPFRYFSEVTNSEDSPEFKQEWLKMKDMYDNLSHKIEDVTLNSTSFQFEGEYAVFLNTEKRNHPSIVKVILENNDGLSDGLPHLVYISREKRPKYEHNYKAGAMNVLTRVSGLMTNAPFMLNVDCDMVVNNAKIIQHAMCILMDSKNGKDVAFVQCFQQFYDGIKDDPFGNQWVASFEYIIKGMGGLQGPFYGGTNTFHRRNAIYGLYPDEIQYGRKGKITEKILIQQFGSSREFVKSVTHAFEGSDNSIDGISPSILLDKAIQVSDCGYEYGTCWGKQMGWLYGSISEDVPTGLNMQRKGWRSECCTPEPTAFMGCAPGGLLTTMIQQKRWSSGLTVVFFSKHSPVMGTLFGKIQFRAGLSYCWLTNWGLRSVFEVSYAALVAYCIITNTSIFPKGLGLWIPLTLFVIYTIHTLQEYLSKGLSLRFWWNNQRMITIRSTSVWFIGFLSGMIKLLGISDTIFEVTQKESPTSGAAEDDANAGRFTFDESPAFVVGTTILLVQFTALVVKILGVQLAAHSGNGCELGELMCSVYLVVCYWPFLKGLFARGKYGIPLSTIFKSALLTFIFVHFSRISVVS*