AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016301.t1 MTR_8g046140 98.343 181 3 0 14 194 1 181 2.27e-131 366
MS.gene016301.t1 MTR_3g007700 98.333 180 3 0 15 194 3 182 1.36e-130 364
MS.gene016301.t1 MTR_4g080740 97.238 181 5 0 14 194 1 181 1.73e-130 363
MS.gene016301.t1 MTR_3g007690 79.348 184 36 2 12 193 58 241 1.83e-101 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016301.t1 AT2G42740 95.580 181 8 0 14 194 1 181 5.52e-128 357
MS.gene016301.t1 AT5G45775 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene016301.t1 AT4G18730 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene016301.t1 AT3G58700 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene016301.t1 AT5G45775 95.906 171 7 0 24 194 1 171 2.69e-121 340

Find 49 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCTTCAACTGGCATTTA+TGG 0.115122 8.2:+56048206 MS.gene016301:CDS
TTTAGTGATACTGGTTGTTT+TGG 0.242495 8.2:+56047683 MS.gene016301:CDS
CGATACACTGTCCGTTCTTT+TGG 0.247965 8.2:+56047548 MS.gene016301:CDS
CGACAAGGCAATGCAGCTTT+TGG 0.255810 8.2:+56047616 MS.gene016301:CDS
GCTTGCTATGTGACTGTTAG+AGG 0.285727 8.2:+56047593 MS.gene016301:CDS
AATTCAGGAGCATATTGATT+TGG 0.303290 8.2:+56047712 MS.gene016301:CDS
TAAAATTACCTTTGCAGCTC+TGG 0.312435 8.2:-56045474 None:intergenic
GAAAAGAAAGCGTACCATTT+GGG 0.312762 8.2:-56045290 None:intergenic
ATTCAGGAGCATATTGATTT+GGG 0.346376 8.2:+56047713 MS.gene016301:CDS
CTTTGGAAAACACTGGGGTT+TGG 0.360701 8.2:-56045585 None:intergenic
CTCAGGTATGATCCTTCAAC+TGG 0.406013 8.2:+56048197 MS.gene016301:intron
TTCGTTGTTCTGGAGCGTCC+TGG 0.441611 8.2:+56048239 MS.gene016301:CDS
CTTCCGTTTCCAACCCCAAA+TGG 0.453177 8.2:+56045276 MS.gene016301:CDS
CTCGTTCTTAATATCTCTGT+TGG 0.461901 8.2:+56045431 MS.gene016301:CDS
CGTCCTTTGTTACGCGATGT+TGG 0.468785 8.2:-56048307 None:intergenic
ATTCCATAAATGCCAGTTGA+AGG 0.482057 8.2:-56048209 None:intergenic
AATATCTCTGTTGGTGAAAG+TGG 0.486734 8.2:+56045440 MS.gene016301:CDS
TTCAACTGGCATTTATGGAA+TGG 0.497930 8.2:+56048211 MS.gene016301:CDS
CTGAACCTTGATATCCCTCA+TGG 0.500440 8.2:-56045405 None:intergenic
AATAAACCTTTGGAAAACAC+TGG 0.500470 8.2:-56045592 None:intergenic
GGAGAGCGGTTTGAAGGTGA+AGG 0.504094 8.2:+56047637 MS.gene016301:CDS
GTACCATTTGGGGTTGGAAA+CGG 0.510966 8.2:-56045279 None:intergenic
TAAGTAACTAAACAATCGCC+TGG 0.515662 8.2:-56048382 None:intergenic
GGGACGTCGTCGTCGGTGCA+AGG 0.515936 8.2:+56048271 MS.gene016301:CDS
TCACACCTTCATATTTCACT+TGG 0.516049 8.2:-56048346 None:intergenic
CGACGTCCCACACGGTAACC+AGG 0.522429 8.2:-56048257 None:intergenic
CAATCGCCTGGGACTTGTTG+AGG 0.523910 8.2:-56048370 None:intergenic
ATAAACCTTTGGAAAACACT+GGG 0.526168 8.2:-56045591 None:intergenic
CGTCGGTGCAAGGCTCGTGT+TGG 0.539971 8.2:+56048281 MS.gene016301:CDS
GAACACCATGAGGGATATCA+AGG 0.543913 8.2:+56045400 MS.gene016301:CDS
GAGCGTCCTGGTTACCGTGT+GGG 0.553181 8.2:+56048251 MS.gene016301:CDS
TTCTTCTAATACCAAAAGAA+CGG 0.565907 8.2:-56047559 None:intergenic
ACCGTGTGGGACGTCGTCGT+CGG 0.567737 8.2:+56048264 MS.gene016301:CDS
TCGTCTCACCAGAGCTGCAA+AGG 0.570040 8.2:+56045466 MS.gene016301:CDS
GAAAGCGTACCATTTGGGGT+TGG 0.573294 8.2:-56045285 None:intergenic
TGGTTCCAAGTGAAATATGA+AGG 0.576763 8.2:+56048341 MS.gene016301:CDS
AAAAGAAAGCGTACCATTTG+GGG 0.586889 8.2:-56045289 None:intergenic
TGTGATCCTCAACAAGTCCC+AGG 0.588999 8.2:+56048364 MS.gene016301:CDS
AATCCAACATCGCGTAACAA+AGG 0.595227 8.2:+56048304 MS.gene016301:CDS
TGGGGTTGGAAACGGAAGAG+AGG 0.621017 8.2:-56045271 None:intergenic
CAAAGGACGATGCTATGAAG+TGG 0.633896 8.2:+56048321 MS.gene016301:CDS
AAGTAACTAAACAATCGCCT+GGG 0.633971 8.2:-56048381 None:intergenic
GGAGCGTCCTGGTTACCGTG+TGG 0.638331 8.2:+56048250 MS.gene016301:CDS
AGAAACTATCGAACACCATG+AGG 0.645935 8.2:+56045390 MS.gene016301:CDS
ACCGACGACGACGTCCCACA+CGG 0.654712 8.2:-56048265 None:intergenic
TGTGACTGTTAGAGGCGACA+AGG 0.664589 8.2:+56047601 MS.gene016301:CDS
GAAACTATCGAACACCATGA+GGG 0.674278 8.2:+56045391 MS.gene016301:CDS
TAAACCTTTGGAAAACACTG+GGG 0.679293 8.2:-56045590 None:intergenic
CAGGTGTTGGAACAACTCAG+TGG 0.734121 8.2:+56045562 MS.gene016301:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTGATTTTAATAGTTTA+GGG + chr8.2:56045956-56045975 MS.gene016301:intron 10.0%
!! AACTATTAAAATCAACAAAA+AGG + chr8.2:56045847-56045866 MS.gene016301:intron 15.0%
!! AATTTAGAAAACAAATGTTA+AGG - chr8.2:56046800-56046819 None:intergenic 15.0%
!! ATAAAAATAAATTGGTGTAT+AGG - chr8.2:56047306-56047325 None:intergenic 15.0%
!! CAAAATTAAAAATAAACCTT+TGG - chr8.2:56045605-56045624 None:intergenic 15.0%
!! CAAGACAAAAATAAAAAATT+GGG - chr8.2:56046285-56046304 None:intergenic 15.0%
!! CTAAACTATTAAAATCAATT+AGG - chr8.2:56045957-56045976 None:intergenic 15.0%
!! TATTTATAAATCATCAACAT+CGG - chr8.2:56046443-56046462 None:intergenic 15.0%
!! TCAAGACAAAAATAAAAAAT+TGG - chr8.2:56046286-56046305 None:intergenic 15.0%
!!! AAAATTTGAAATTATGTCTT+TGG + chr8.2:56048066-56048085 MS.gene016301:intron 15.0%
!!! AAGATTGTTGATAAAAATTA+AGG + chr8.2:56046044-56046063 MS.gene016301:intron 15.0%
!!! AGATTGTTGATAAAAATTAA+GGG + chr8.2:56046045-56046064 MS.gene016301:intron 15.0%
!!! CTAATTGATTTTAATAGTTT+AGG + chr8.2:56045955-56045974 MS.gene016301:intron 15.0%
!!! TATGATTGTTTATAAGTTTT+AGG + chr8.2:56047046-56047065 MS.gene016301:intron 15.0%
!!! TATTATTTTACCTTGAAAAT+TGG + chr8.2:56047323-56047342 MS.gene016301:intron 15.0%
!!! TATTTTTGTCTTGAAAAAAA+TGG + chr8.2:56046292-56046311 MS.gene016301:intron 15.0%
!!! TCAAAGTTTTACATAATTTT+AGG + chr8.2:56047963-56047982 MS.gene016301:intron 15.0%
!!! TTTCATTTTTTGTATTTATC+AGG + chr8.2:56045543-56045562 MS.gene016301:intron 15.0%
!! AACATAAATGATAACAAACT+TGG - chr8.2:56048162-56048181 None:intergenic 20.0%
!! AACTATTAAAATCAATTAGG+AGG - chr8.2:56045954-56045973 None:intergenic 20.0%
!! CTAATAGATATGAGAAAATT+TGG - chr8.2:56047474-56047493 None:intergenic 20.0%
!! TAATGTTAATGATGATAGAA+TGG + chr8.2:56047268-56047287 MS.gene016301:intron 20.0%
!! TATTCAATATACAATCAACT+AGG - chr8.2:56045778-56045797 None:intergenic 20.0%
!! TGATTTGAATGAGAATAAAA+AGG + chr8.2:56046631-56046650 MS.gene016301:intron 20.0%
!!! ATCACACATTTTATTATTTG+AGG - chr8.2:56046344-56046363 None:intergenic 20.0%
!!! GATTGTGTTTTTTTACTAAA+AGG + chr8.2:56046538-56046557 MS.gene016301:intron 20.0%
!!! GTTTTACATAATTTTAGGAT+AGG + chr8.2:56047968-56047987 MS.gene016301:intron 20.0%
!!! TAATCATTTAGTTCTACAAA+AGG + chr8.2:56047869-56047888 MS.gene016301:intron 20.0%
!!! TTCTTCTGGTATTTTAATTT+AGG - chr8.2:56046727-56046746 None:intergenic 20.0%
! AAAAACAACAATCAGTTAAC+TGG - chr8.2:56047505-56047524 None:intergenic 25.0%
! AAAACAACCAAAAACTTTCA+AGG - chr8.2:56046160-56046179 None:intergenic 25.0%
! AAACTATGGTTTAAGCTATT+AGG + chr8.2:56047937-56047956 MS.gene016301:intron 25.0%
! ATAAATGATAACAAACTTGG+TGG - chr8.2:56048159-56048178 None:intergenic 25.0%
! ATTACCATTCTATTAGATAC+TGG + chr8.2:56046938-56046957 MS.gene016301:intron 25.0%
! GAATGAGAATAAAAAGGAAA+GGG + chr8.2:56046637-56046656 MS.gene016301:intron 25.0%
! GATGGAATATCATTAGAAAT+CGG - chr8.2:56048135-56048154 None:intergenic 25.0%
! TGAAATGATAAAACACTCAA+AGG - chr8.2:56048095-56048114 None:intergenic 25.0%
! TGAATGAGAATAAAAAGGAA+AGG + chr8.2:56046636-56046655 MS.gene016301:intron 25.0%
! TTCTTCTAATACCAAAAGAA+CGG - chr8.2:56047562-56047581 None:intergenic 25.0%
! TTCTTCTGGTATTTACATTT+AGG - chr8.2:56046134-56046153 None:intergenic 25.0%
!! AAAACAACCAAAAGTTTTCA+AGG - chr8.2:56047202-56047221 None:intergenic 25.0%
!! AGAAAATCCTTGAAAACTTT+TGG + chr8.2:56047192-56047211 MS.gene016301:intron 25.0%
!! GTTAAATTGCATTGATAGAA+AGG + chr8.2:56046223-56046242 MS.gene016301:intron 25.0%
!! TGCTAAATAATCAAGTTTTC+TGG + chr8.2:56046910-56046929 MS.gene016301:intron 25.0%
!! TTCTTCTGGTATTTTCATTT+AGG - chr8.2:56047176-56047195 None:intergenic 25.0%
!! TTTAACATTGTTTCTTTCTC+AGG + chr8.2:56048180-56048199 MS.gene016301:intron 25.0%
!! TTTTTGTATTTATCAGGTGT+TGG + chr8.2:56045549-56045568 MS.gene016301:intron 25.0%
!!! AGAAGAAATTTTAGTGATAC+TGG + chr8.2:56047674-56047693 MS.gene016301:CDS 25.0%
!!! CTGTTGACTCAATTATAAAA+TGG - chr8.2:56046665-56046684 None:intergenic 25.0%
!!! TTTTCGAGTCACAATAATTT+GGG + chr8.2:56047218-56047237 MS.gene016301:intron 25.0%
!!! TTTTTTTTTTTGCTGCACAA+AGG + chr8.2:56046479-56046498 MS.gene016301:intron 25.0%
AACTTGATTATTTAGCATGC+AGG - chr8.2:56046907-56046926 None:intergenic 30.0%
AATGGTTGAAAACGATAGAA+TGG + chr8.2:56046310-56046329 MS.gene016301:intron 30.0%
ACTCAAAACATGTGACATTT+AGG - chr8.2:56046856-56046875 None:intergenic 30.0%
AGAAGAGGAAAATAAAAGCA+AGG - chr8.2:56046975-56046994 None:intergenic 30.0%
AGTTCTACAAAAGGAAACTA+TGG + chr8.2:56047923-56047942 MS.gene016301:intron 30.0%
ATTATAATTGAGTCACAGCA+GGG + chr8.2:56047116-56047135 MS.gene016301:intron 30.0%
GGAATGAAAATCAGAAAGAA+TGG - chr8.2:56045315-56045334 None:intergenic 30.0%
TAATAGTTTAGGGACTAAAC+TGG + chr8.2:56045966-56045985 MS.gene016301:intron 30.0%
TATATAATGAGTCAACAGCA+GGG + chr8.2:56046074-56046093 MS.gene016301:intron 30.0%
TATTATAATTGAGTCACAGC+AGG + chr8.2:56047115-56047134 MS.gene016301:intron 30.0%
TCTCATATCTATTAGATGCT+GGG + chr8.2:56047479-56047498 MS.gene016301:intron 30.0%
TTAACGAGAATTGAACTCAA+GGG + chr8.2:56047089-56047108 MS.gene016301:intron 30.0%
TTATAATTGAGTCAACAGCA+GGG + chr8.2:56046667-56046686 MS.gene016301:intron 30.0%
TTATATAATGAGTCAACAGC+AGG + chr8.2:56046073-56046092 MS.gene016301:intron 30.0%
TTCTCATATCTATTAGATGC+TGG + chr8.2:56047478-56047497 MS.gene016301:intron 30.0%
TTTAACGAGAATTGAACTCA+AGG + chr8.2:56047088-56047107 MS.gene016301:intron 30.0%
TTTATAATTGAGTCAACAGC+AGG + chr8.2:56046666-56046685 MS.gene016301:intron 30.0%
! AATAAACCTTTGGAAAACAC+TGG - chr8.2:56045595-56045614 None:intergenic 30.0%
! AATTCAGGAGCATATTGATT+TGG + chr8.2:56047712-56047731 MS.gene016301:CDS 30.0%
! AGAAGATCCTTGAAAGTTTT+TGG + chr8.2:56046150-56046169 MS.gene016301:intron 30.0%
! ATAAACCTTTGGAAAACACT+GGG - chr8.2:56045594-56045613 None:intergenic 30.0%
! ATGCATTTTCTTACAGGTTT+CGG + chr8.2:56045364-56045383 MS.gene016301:intron 30.0%
! CGAAAAATGCATTTTCTTAC+AGG + chr8.2:56045358-56045377 MS.gene016301:intron 30.0%
! TCCTTCAATTTGGTTTACTT+CGG + chr8.2:56045890-56045909 MS.gene016301:intron 30.0%
! TTTCGAGTCACAATAATTTG+GGG + chr8.2:56047219-56047238 MS.gene016301:intron 30.0%
!! AGTTTTCAAGGATTTTCTTC+TGG - chr8.2:56047190-56047209 None:intergenic 30.0%
!! ATTCAGGAGCATATTGATTT+GGG + chr8.2:56047713-56047732 MS.gene016301:CDS 30.0%
!! CGATTGTTTTGTATATCAGT+AGG + chr8.2:56046563-56046582 MS.gene016301:intron 30.0%
!! GTTTTCAAGACACAATTATC+TGG + chr8.2:56046175-56046194 MS.gene016301:intron 30.0%
!! TTTAGTGATACTGGTTGTTT+TGG + chr8.2:56047683-56047702 MS.gene016301:CDS 30.0%
!!! ATTTTAATTTAGGCTCCAAG+CGG - chr8.2:56046717-56046736 None:intergenic 30.0%
!!! GTTTTCGAGTCACAATAATT+TGG + chr8.2:56047217-56047236 MS.gene016301:intron 30.0%
AACTTTCAAGGATCTTCTTC+TGG - chr8.2:56046148-56046167 None:intergenic 35.0%
ACACTGTACGTGAATATTAC+AGG + chr8.2:56047015-56047034 MS.gene016301:intron 35.0%
AGCACCAGTATCTAATAGAA+TGG - chr8.2:56046945-56046964 None:intergenic 35.0%
ATATAATGAGTCAACAGCAG+GGG + chr8.2:56046075-56046094 MS.gene016301:intron 35.0%
ATTCCATAAATGCCAGTTGA+AGG - chr8.2:56048212-56048231 None:intergenic 35.0%
ATTTACATTTAGGCTCCAAG+CGG - chr8.2:56046124-56046143 None:intergenic 35.0%
CTCGTTCTTAATATCTCTGT+TGG + chr8.2:56045431-56045450 MS.gene016301:CDS 35.0%
GCTGTCAATAAGAAAAGAAG+AGG - chr8.2:56046990-56047009 None:intergenic 35.0%
GTGTGATCCATGTTTAATAG+TGG + chr8.2:56046357-56046376 MS.gene016301:intron 35.0%
GTGTTTATCACAATGCATGT+TGG + chr8.2:56047990-56048009 MS.gene016301:intron 35.0%
TAAAATTACCTTTGCAGCTC+TGG - chr8.2:56045477-56045496 None:intergenic 35.0%
TATAATTGAGTCAACAGCAG+GGG + chr8.2:56046668-56046687 MS.gene016301:intron 35.0%
TCACACCTTCATATTTCACT+TGG - chr8.2:56048349-56048368 None:intergenic 35.0%
TCAGAAGACAACAACAACAA+AGG - chr8.2:56045336-56045355 None:intergenic 35.0%
TCCGAAGTAAACCAAATTGA+AGG - chr8.2:56045894-56045913 None:intergenic 35.0%
TGAAAATTGGTCGAAGATGA+TGG + chr8.2:56047336-56047355 MS.gene016301:intron 35.0%
TGGTTCCAAGTGAAATATGA+AGG + chr8.2:56048341-56048360 MS.gene016301:CDS 35.0%
TTATAATTGAGTCACAGCAG+GGG + chr8.2:56047117-56047136 MS.gene016301:intron 35.0%
TTCAACTGGCATTTATGGAA+TGG + chr8.2:56048211-56048230 MS.gene016301:CDS 35.0%
TTGTTTAAACCAGTTTCGCA+TGG - chr8.2:56047807-56047826 None:intergenic 35.0%
! AAAAGAAAGCGTACCATTTG+GGG - chr8.2:56045292-56045311 None:intergenic 35.0%
! AATATCTCTGTTGGTGAAAG+TGG + chr8.2:56045440-56045459 MS.gene016301:CDS 35.0%
! ATTTTCATTTAGGCTCCAAG+CGG - chr8.2:56047166-56047185 None:intergenic 35.0%
! GAAAAGAAAGCGTACCATTT+GGG - chr8.2:56045293-56045312 None:intergenic 35.0%
! TAAACCTTTGGAAAACACTG+GGG - chr8.2:56045593-56045612 None:intergenic 35.0%
! TCAATTTGGTTTACTTCGGA+GGG + chr8.2:56045894-56045913 MS.gene016301:intron 35.0%
! TCATCTTCGACCAATTTTCA+AGG - chr8.2:56047336-56047355 None:intergenic 35.0%
! TTCAATTTGGTTTACTTCGG+AGG + chr8.2:56045893-56045912 MS.gene016301:intron 35.0%
!!! AATGGACTTTTTCGTTGTTC+TGG + chr8.2:56048229-56048248 MS.gene016301:CDS 35.0%
!!! AGGATGAGACGTACATTTTT+TGG - chr8.2:56045874-56045893 None:intergenic 35.0%
!!! GATACTGGTTGTTTTGGATT+CGG + chr8.2:56047689-56047708 MS.gene016301:CDS 35.0%
!!! TTGTTTTGGATTCGGAATTC+AGG + chr8.2:56047697-56047716 MS.gene016301:CDS 35.0%
AATCCAACATCGCGTAACAA+AGG + chr8.2:56048304-56048323 MS.gene016301:CDS 40.0%
AATGGGTGGATGCACATTTA+AGG + chr8.2:56045926-56045945 MS.gene016301:intron 40.0%
AGAAACTATCGAACACCATG+AGG + chr8.2:56045390-56045409 MS.gene016301:CDS 40.0%
ATGGGTGGATGCACATTTAA+GGG + chr8.2:56045927-56045946 MS.gene016301:intron 40.0%
CAAGCAGCCACTATTAAACA+TGG - chr8.2:56046367-56046386 None:intergenic 40.0%
CTATTTCAGCACAAAACACG+TGG - chr8.2:56047400-56047419 None:intergenic 40.0%
GAAACTATCGAACACCATGA+GGG + chr8.2:56045391-56045410 MS.gene016301:CDS 40.0%
GATCCTTCAACTGGCATTTA+TGG + chr8.2:56048206-56048225 MS.gene016301:CDS 40.0%
GTACGTCTCATCCTTCAATT+TGG + chr8.2:56045880-56045899 MS.gene016301:intron 40.0%
TCGGAGGGACTAAATTGAAT+GGG + chr8.2:56045909-56045928 MS.gene016301:intron 40.0%
TTCGGAGGGACTAAATTGAA+TGG + chr8.2:56045908-56045927 MS.gene016301:intron 40.0%
! GGAAAAGAAAGCGTACCATT+TGG - chr8.2:56045294-56045313 None:intergenic 40.0%
!! GATAACAAACTTGGTGGTGA+TGG - chr8.2:56048153-56048172 None:intergenic 40.0%
CAAAGGACGATGCTATGAAG+TGG + chr8.2:56048321-56048340 MS.gene016301:CDS 45.0%
CTCAGGTATGATCCTTCAAC+TGG + chr8.2:56048197-56048216 MS.gene016301:intron 45.0%
CTGAACCTTGATATCCCTCA+TGG - chr8.2:56045408-56045427 None:intergenic 45.0%
GAACACCATGAGGGATATCA+AGG + chr8.2:56045400-56045419 MS.gene016301:CDS 45.0%
GAGGGACTAAATTGAATGGG+TGG + chr8.2:56045912-56045931 MS.gene016301:intron 45.0%
GGCGAGAGAGTGATAGTTTA+GGG + chr8.2:56045987-56046006 MS.gene016301:intron 45.0%
TAGTATGTGCCATGCGAAAC+TGG + chr8.2:56047795-56047814 MS.gene016301:intron 45.0%
TGGCGAGAGAGTGATAGTTT+AGG + chr8.2:56045986-56046005 MS.gene016301:intron 45.0%
! CAAACCCCAGTGTTTTCCAA+AGG + chr8.2:56045586-56045605 MS.gene016301:CDS 45.0%
! CGATACACTGTCCGTTCTTT+TGG + chr8.2:56047548-56047567 MS.gene016301:CDS 45.0%
! CTTTGGAAAACACTGGGGTT+TGG - chr8.2:56045588-56045607 None:intergenic 45.0%
! GTACCATTTGGGGTTGGAAA+CGG - chr8.2:56045282-56045301 None:intergenic 45.0%
!! GCTTGCTATGTGACTGTTAG+AGG + chr8.2:56047593-56047612 MS.gene016301:CDS 45.0%
!! GTAAAATAATAAAAATAAAT+TGG - chr8.2:56047314-56047333 None:intergenic 5.0%
ATGACTACTTGACTGCCGCT+TGG + chr8.2:56046699-56046718 MS.gene016301:intron 50.0%
CGTCCTTTGTTACGCGATGT+TGG - chr8.2:56048310-56048329 None:intergenic 50.0%
CTTCCGTTTCCAACCCCAAA+TGG + chr8.2:56045276-56045295 MS.gene016301:CDS 50.0%
TGTGACTGTTAGAGGCGACA+AGG + chr8.2:56047601-56047620 MS.gene016301:CDS 50.0%
TGTGATCCTCAACAAGTCCC+AGG + chr8.2:56048364-56048383 MS.gene016301:CDS 50.0%
! CGACAAGGCAATGCAGCTTT+TGG + chr8.2:56047616-56047635 MS.gene016301:CDS 50.0%
! GAAAGCGTACCATTTGGGGT+TGG - chr8.2:56045288-56045307 None:intergenic 50.0%
!! CAGGTGTTGGAACAACTCAG+TGG + chr8.2:56045562-56045581 MS.gene016301:intron 50.0%
!! GCAATGCAGCTTTTGGAGAG+CGG + chr8.2:56047623-56047642 MS.gene016301:CDS 50.0%
!! GCTTTTGGAGAGCGGTTTGA+AGG + chr8.2:56047631-56047650 MS.gene016301:CDS 50.0%
ATGACTACCTGACTGCCGCT+TGG + chr8.2:56046106-56046125 MS.gene016301:intron 55.0%
GGAGAGCGGTTTGAAGGTGA+AGG + chr8.2:56047637-56047656 MS.gene016301:CDS 55.0%
TCGTCTCACCAGAGCTGCAA+AGG + chr8.2:56045466-56045485 MS.gene016301:CDS 55.0%
TGGGGTTGGAAACGGAAGAG+AGG - chr8.2:56045274-56045293 None:intergenic 55.0%
!! CAATCGCCTGGGACTTGTTG+AGG - chr8.2:56048373-56048392 None:intergenic 55.0%
!! TTCGTTGTTCTGGAGCGTCC+TGG + chr8.2:56048239-56048258 MS.gene016301:CDS 55.0%
ATGACTGCCTGACTGCCGCT+TGG + chr8.2:56047148-56047167 MS.gene016301:intron 60.0%
GAGCGTCCTGGTTACCGTGT+GGG + chr8.2:56048251-56048270 MS.gene016301:CDS 60.0%
TTAGGCTCCAAGCGGCAGTC+AGG - chr8.2:56046116-56046135 None:intergenic 60.0%
ACCGACGACGACGTCCCACA+CGG - chr8.2:56048268-56048287 None:intergenic 65.0%
ACCGTGTGGGACGTCGTCGT+CGG + chr8.2:56048264-56048283 MS.gene016301:CDS 65.0%
CGACGTCCCACACGGTAACC+AGG - chr8.2:56048260-56048279 None:intergenic 65.0%
CGTCGGTGCAAGGCTCGTGT+TGG + chr8.2:56048281-56048300 MS.gene016301:CDS 65.0%
! GGAGCGTCCTGGTTACCGTG+TGG + chr8.2:56048250-56048269 MS.gene016301:CDS 65.0%
GGGACGTCGTCGTCGGTGCA+AGG + chr8.2:56048271-56048290 MS.gene016301:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 56045256 56048397 56045256 ID=MS.gene016301
chr8.2 mRNA 56045256 56048397 56045256 ID=MS.gene016301.t1;Parent=MS.gene016301
chr8.2 exon 56045256 56045297 56045256 ID=MS.gene016301.t1.exon1;Parent=MS.gene016301.t1
chr8.2 CDS 56045256 56045297 56045256 ID=cds.MS.gene016301.t1;Parent=MS.gene016301.t1
chr8.2 exon 56045380 56045487 56045380 ID=MS.gene016301.t1.exon2;Parent=MS.gene016301.t1
chr8.2 CDS 56045380 56045487 56045380 ID=cds.MS.gene016301.t1;Parent=MS.gene016301.t1
chr8.2 exon 56045565 56045607 56045565 ID=MS.gene016301.t1.exon3;Parent=MS.gene016301.t1
chr8.2 CDS 56045565 56045607 56045565 ID=cds.MS.gene016301.t1;Parent=MS.gene016301.t1
chr8.2 exon 56047546 56047741 56047546 ID=MS.gene016301.t1.exon4;Parent=MS.gene016301.t1
chr8.2 CDS 56047546 56047741 56047546 ID=cds.MS.gene016301.t1;Parent=MS.gene016301.t1
chr8.2 exon 56048202 56048397 56048202 ID=MS.gene016301.t1.exon5;Parent=MS.gene016301.t1
chr8.2 CDS 56048202 56048397 56048202 ID=cds.MS.gene016301.t1;Parent=MS.gene016301.t1
Gene Sequence

>MS.gene016301

ATGGCACAACAACAACCTCTCTTCCGTTTCCAACCCCAAATGGTACGCTTTCTTTTCCATTCTTTCTGATTTTCATTCCTTTGTTGTTGTTGTCTTCTGAATCGAAAAATGCATTTTCTTACAGGTTTCGGAGAAGAAACTATCGAACACCATGAGGGATATCAAGGTTCAGAAACTCGTTCTTAATATCTCTGTTGGTGAAAGTGGCGATCGTCTCACCAGAGCTGCAAAGGTAATTTTAATTTTTACTTTTTTTTTTTAATTTTATATATTTTTTTATTCTTACATTTCATTTTTTGTATTTATCAGGTGTTGGAACAACTCAGTGGCCAAACCCCAGTGTTTTCCAAAGGTTTATTTTTAATTTTGATTTTTTTTTTGCTATCTTAATTTGATTTTCAACTTTAAATTAAATAAATCTAGACTTTATTTTTATTTAAATATTAACATGTATGTATATGTATATTCATTGATGTTAATGTAATTGATAGTATTTTTGTGCTTAATGTGAATCAATATCCTAGTTGATTGTATATTGAATAAACAGTCAATTTAGTTTTTAAACTATTACTCTCTTCAATTCGATTCGTAAACTATTAAAATCAACAAAAAGGTCCAAAAAATGTACGTCTCATCCTTCAATTTGGTTTACTTCGGAGGGACTAAATTGAATGGGTGGATGCACATTTAAGGGACCTCCTAATTGATTTTAATAGTTTAGGGACTAAACTGGCGAGAGAGTGATAGTTTAGGGACTAAATTGACTGTTTATTCTCTTATATTTGAATAAGATTGTTGATAAAAATTAAGGGCTCTATTATATAATGAGTCAACAGCAGGGGTCTGTTTAATGACTACCTGACTGCCGCTTGGAGCCTAAATGTAAATACCAGAAGAAGATCCTTGAAAGTTTTTGGTTGTTTTCAAGACACAATTATCTGGTGAAATTCTTACGTTTGTATCATATGTTAAATTGCATTGATAGAAAGGTACTTTATAATCTCATCAAATTGATTTTCATATGTTCCCAATTTTTTATTTTTGTCTTGAAAAAAATGGTTGAAAACGATAGAATGGTTATATATCCTCAAATAATAAAATGTGTGATCCATGTTTAATAGTGGCTGCTTGACTTGTTTTTGATTTGTATGTGTTTGTGTATTGCGTCGATGTTTTTCATTACACCGATGTTGATGATTTATAAATATAATTGATAGTATTTTTTTTTTTTTTTGCTGCACAAAGGAGTAACAAGAAATGTGAAATATCACAGTTTAGTAGTGATTGTGTTTTTTTACTAAAAGGAGCGATTGTTTTGTATATCAGTAGGATCATTGAAATGATAGTTCATAAGTTTATGAATTTGATCAAATTATGATTTGAATGAGAATAAAAAGGAAAGGGCTCCATTTTATAATTGAGTCAACAGCAGGGGTCTGTTTAATGACTACTTGACTGCCGCTTGGAGCCTAAATTAAAATACCAGAAGAAGATCCTTGAAAGTTTTTGGTTGTTTTCAAGACACAATTATCTGGCGAAATCCTTAACATTTGTTTTCTAAATTGTAATACTATAATTCATAGCTTAAATTAAAATGCCTAAATGTCACATGTTTTGAGTTGAAACAGTTTTTGTTGAATTCGTGTTTCCTGCATGCTAAATAATCAAGTTTTCTGGCTGCAATTACCATTCTATTAGATACTGGTGCTGATTGTTCCTTGCTTTTATTTTCCTCTTCTTTTCTTATTGACAGCAAGTTACACTGTACGTGAATATTACAGGATTATTGATATGATTGTTTATAAGTTTTAGGATCTTGAACAAAATATGTTTTTAACGAGAATTGAACTCAAGGGCTCTATTATAATTGAGTCACAGCAGGGGTCTGTTTAATGACTGCCTGACTGCCGCTTGGAGCCTAAATGAAAATACCAGAAGAAAATCCTTGAAAACTTTTGGTTGTTTTCGAGTCACAATAATTTGGGGAAATTATTACATTTGTATCATATCTTTAATGTTAATGATGATAGAATGGTGCTTTAATTTTCCTATACACCAATTTATTTTTATTATTTTACCTTGAAAATTGGTCGAAGATGATGGAATGATTATTTATTCTTGAATGATAAAATGTGTAAATTCCACGTGTTTTGTGCTGAAATAGCTTGTGTTGCTTTCATGCTAAGAAATCAAGTCTAGTTGCAATTATCGATCGCCAAATTTTCTCATATCTATTAGATGCTGGGCCAGTTAACTGATTGTTGTTTTTCTTTCTCTGTCTTTTTGACAGCTCGATACACTGTCCGTTCTTTTGGTATTAGAAGAAATGAAAAGATTGCTTGCTATGTGACTGTTAGAGGCGACAAGGCAATGCAGCTTTTGGAGAGCGGTTTGAAGGTGAAGGAATATGAATTGCTGAGAAGAAATTTTAGTGATACTGGTTGTTTTGGATTCGGAATTCAGGAGCATATTGATTTGGGAATCAAGTAAGCTCTAAATATCTTGTATCTGTAACTGAAGTATATGTGCACGTTATACTTAGTATGTGCCATGCGAAACTGGTTTAAACAAACTTTTCTAGAATGTTGCTTTATTTATGTTTCATTTATCTACTAATCATTTAGTTCTACAAAAGGAATAAAGCATTCATTTATCTACTAATCATTTAGTTCTACAAAAGGAAACTATGGTTTAAGCTATTAGGTGTTCAAAGTTTTACATAATTTTAGGATAGGTGTTTATCACAATGCATGTTGGATTTGAAGTTATGAGTTGAGTTAATCTGATTTTTGTCTTTTGAGTTTATTATAAAAATTTGAAATTATGTCTTTGGTTTCCTTTGAGTGTTTTATCATTTCAGTCTTTAAGCTTATAATCCGATTTCTAATGATATTCCATCACCACCAAGTTTGTTATCATTTATGTTTAACATTGTTTCTTTCTCAGGTATGATCCTTCAACTGGCATTTATGGAATGGACTTTTTCGTTGTTCTGGAGCGTCCTGGTTACCGTGTGGGACGTCGTCGTCGGTGCAAGGCTCGTGTTGGAATCCAACATCGCGTAACAAAGGACGATGCTATGAAGTGGTTCCAAGTGAAATATGAAGGTGTGATCCTCAACAAGTCCCAGGCGATTGTTTAG
Protein sequence

>MS.gene016301.t1

MAQQQPLFRFQPQMVSEKKLSNTMRDIKVQKLVLNISVGESGDRLTRAAKVLEQLSGQTPVFSKARYTVRSFGIRRNEKIACYVTVRGDKAMQLLESGLKVKEYELLRRNFSDTGCFGFGIQEHIDLGIKYDPSTGIYGMDFFVVLERPGYRVGRRRRCKARVGIQHRVTKDDAMKWFQVKYEGVILNKSQAIV