AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071916

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071916.t1 MTR_2g008870 94.562 331 16 2 1 329 1 331 0.0 629
MS.gene071916.t1 MTR_2g008870 95.423 284 12 1 1 283 1 284 0.0 555
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071916.t1 AT2G37990 60.308 325 120 3 1 324 1 317 1.72e-130 375

Find 81 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAAAATTACTGTGCTTT+TGG 0.169446 2.2:+71459134 None:intergenic
GGCCTAAACCTCCTACTAAA+TGG 0.206025 2.2:-71461624 MS.gene071916:CDS
CGATTCTTTCCTTTATGTTT+TGG 0.214740 2.2:+71459604 None:intergenic
GAGTATATCATGATTATTCT+TGG 0.294298 2.2:+71459380 None:intergenic
GTTAAGGAGTGTTTACAAAA+AGG 0.309485 2.2:-71461897 MS.gene071916:CDS
GGTGCGTGGGTTCCTGTTAT+AGG 0.317411 2.2:+71459733 None:intergenic
GCGACTTCTTTGCCTATAAC+AGG 0.351994 2.2:-71459745 MS.gene071916:CDS
GTAGGTTTAGCCTCAATAAT+AGG 0.359792 2.2:+71461356 None:intergenic
CTCTGGGTAATCTGGTGGAT+GGG 0.363308 2.2:+71461787 None:intergenic
TCCCATTTAGTAGGAGGTTT+AGG 0.365899 2.2:+71461622 None:intergenic
ATATCCATGTCTGCGTTTCC+AGG 0.400440 2.2:+71461406 None:intergenic
TATTAGGGTTGAAGTGGTTA+AGG 0.401973 2.2:-71461913 MS.gene071916:intron
CCTTCGATTTATCATTCCTT+CGG 0.407735 2.2:+71459225 None:intergenic
CCCTCTACTCAAGATGTTGA+AGG 0.416054 2.2:-71461831 MS.gene071916:CDS
CTGCCGGTAGCAGAAGGAAC+AGG 0.421319 2.2:-71459456 MS.gene071916:CDS
CCAAGAGAAAAGAAAACAAA+AGG 0.423905 2.2:-71460684 MS.gene071916:CDS
TCTCTGGGTAATCTGGTGGA+TGG 0.423953 2.2:+71461786 None:intergenic
TTAGTAGGAGGTTTAGGCCT+TGG 0.426653 2.2:+71461628 None:intergenic
GCAAACAGGTCCCATTTAGT+AGG 0.428944 2.2:+71461613 None:intergenic
GAAAAGCCTCCAAAACATAA+AGG 0.437070 2.2:-71459613 MS.gene071916:CDS
GGTTGACCTCGGTAATCTAA+TGG 0.449578 2.2:-71462079 MS.gene071916:CDS
GGTAATCTGGTGGATGGGGC+AGG 0.450665 2.2:+71461792 None:intergenic
GCCTAAACCTCCTACTAAAT+GGG 0.452325 2.2:-71461623 MS.gene071916:CDS
CAAGTTTATGATGAACCATC+TGG 0.452578 2.2:-71461431 MS.gene071916:CDS
TTGGCAAAAGGATCCTCGTC+TGG 0.469582 2.2:+71460703 None:intergenic
TCTGGGTAATCTGGTGGATG+GGG 0.481064 2.2:+71461788 None:intergenic
ACCCCTGTTCCTTCTGCTAC+CGG 0.485546 2.2:+71459453 None:intergenic
GCCTCAACTGCCACAGCTAG+TGG 0.485917 2.2:-71459664 MS.gene071916:CDS
AGGAACAGGGGTAGGATCAA+AGG 0.493785 2.2:-71459442 MS.gene071916:CDS
GGCTGCTAAGAATTTGTTCG+AGG 0.502076 2.2:-71462100 MS.gene071916:CDS
CAAGAGAAAAGAAAACAAAA+GGG 0.503360 2.2:-71460683 MS.gene071916:CDS
AAGGTTACCAAGGATGAATT+AGG 0.504736 2.2:-71459703 MS.gene071916:CDS
GATGAATTAGGCAGTGTAGC+AGG 0.505642 2.2:-71459691 MS.gene071916:CDS
CACAGTGACCAATGTGAAGA+AGG 0.507110 2.2:-71459255 MS.gene071916:CDS
CTGCGTTTCCAGGTTCCAGA+TGG 0.508540 2.2:+71461416 None:intergenic
TACACTGCCTAATTCATCCT+TGG 0.511559 2.2:+71459696 None:intergenic
ATGATGAACCATCTGGAACC+TGG 0.518041 2.2:-71461424 MS.gene071916:CDS
AACCCACGCACCACAAAAGA+AGG 0.519084 2.2:-71459722 MS.gene071916:CDS
AAAGAAAATGGAGATTGAGA+TGG 0.520851 2.2:-71462124 None:intergenic
AGAAGCTATTCCTATTATTG+AGG 0.529821 2.2:-71461366 MS.gene071916:CDS
CAAAGTTTGGGGCTTTACCT+AGG 0.530560 2.2:-71460609 MS.gene071916:intron
GAAAATGGAGATTGAGATGG+AGG 0.531102 2.2:-71462121 None:intergenic
ACCACTAGCTGTGGCAGTTG+AGG 0.531694 2.2:+71459663 None:intergenic
TCTTCTCATCTAGTTCCTGC+CGG 0.534818 2.2:-71459472 MS.gene071916:intron
CATACCCTTCTCAACAATCC+AGG 0.537224 2.2:-71462038 MS.gene071916:intron
AACAGGTCCCATTTAGTAGG+AGG 0.542260 2.2:+71461616 None:intergenic
ACCATGCAGCCATGTTCAGT+TGG 0.543010 2.2:-71459770 MS.gene071916:intron
ATTCTCTATTAGGGTTGAAG+TGG 0.546834 2.2:-71461919 MS.gene071916:intron
TAGTTCCTGCCGGTAGCAGA+AGG 0.558864 2.2:-71459462 MS.gene071916:CDS
ACATACCTGGATTGTTGAGA+AGG 0.562948 2.2:+71462033 None:intergenic
CCTTCAACATCTTGAGTAGA+GGG 0.564606 2.2:+71461831 None:intergenic
AAGTCGCGGCCAACTGAACA+TGG 0.566896 2.2:+71459761 None:intergenic
TCAACTGCCACAGCTAGTGG+TGG 0.576419 2.2:-71459661 MS.gene071916:CDS
TGGAATTAGTTACCATCGGT+AGG 0.578433 2.2:+71461338 None:intergenic
CTGTTCCTTCTGCTACCGGC+AGG 0.578497 2.2:+71459457 None:intergenic
ATGGGGCAGGCAATTGAACG+AGG 0.586894 2.2:+71461805 None:intergenic
ACCTTCAACATCTTGAGTAG+AGG 0.587465 2.2:+71461830 None:intergenic
TGGGGCAGGCAATTGAACGA+GGG 0.602818 2.2:+71461806 None:intergenic
TGCCGGTAGCAGAAGGAACA+GGG 0.605130 2.2:-71459455 MS.gene071916:CDS
CATACCTGGATTGTTGAGAA+GGG 0.605884 2.2:+71462034 None:intergenic
GGAACCTGGAAACGCAGACA+TGG 0.610484 2.2:-71461410 MS.gene071916:CDS
AAGAGAAAAGAAAACAAAAG+GGG 0.617318 2.2:-71460682 MS.gene071916:CDS
AGGGGTAGGATCAAAGGAGA+GGG 0.618168 2.2:-71459436 MS.gene071916:CDS
AGGCATTATTAAGCGGAACA+AGG 0.619070 2.2:-71461459 MS.gene071916:CDS
CAACTGCCACAGCTAGTGGT+GGG 0.621815 2.2:-71459660 MS.gene071916:CDS
GTTATGCAGGCATTATTAAG+CGG 0.622663 2.2:-71461466 MS.gene071916:intron
GGGGCAGGCAATTGAACGAG+GGG 0.623547 2.2:+71461807 None:intergenic
GTAGCAGAAGGAACAGGGGT+AGG 0.632666 2.2:-71459450 MS.gene071916:CDS
CCGAAGGAATGATAAATCGA+AGG 0.634190 2.2:-71459225 MS.gene071916:CDS
AAACTTCCCACCACTAGCTG+TGG 0.634673 2.2:+71459654 None:intergenic
GTATGAATGATGAGAGTCGA+AGG 0.637332 2.2:+71462056 None:intergenic
AATTTGTTCGAGGTTGACCT+CGG 0.638182 2.2:-71462090 MS.gene071916:CDS
TGATATACTCAATGTTAACA+AGG 0.643030 2.2:-71459367 MS.gene071916:intron
CAGGGGTAGGATCAAAGGAG+AGG 0.644223 2.2:-71459437 MS.gene071916:CDS
ATTGAGGCTAAACCTACCGA+TGG 0.658675 2.2:-71461350 MS.gene071916:intron
TGTTATAGGCAAAGAAGTCG+CGG 0.661140 2.2:+71459747 None:intergenic
TGAAGAAGGAGAAAAGCCGA+AGG 0.662135 2.2:-71459241 MS.gene071916:CDS
ACATAAAGGAAAGAATCGAA+AGG 0.670337 2.2:-71459599 MS.gene071916:intron
ACCACAAAAGAAGGTTACCA+AGG 0.679267 2.2:-71459713 MS.gene071916:CDS
GCCGGTAGCAGAAGGAACAG+GGG 0.714140 2.2:-71459454 MS.gene071916:CDS
CGAAGGCCATTAGATTACCG+AGG 0.720268 2.2:+71462073 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTGAAAATTCTTTA+TGG + chr2.2:71461319-71461338 None:intergenic 10.0%
!! AAACTATTTATAAATGTTAA+GGG - chr2.2:71460384-71460403 MS.gene071916:intron 10.0%
!! AAAAAATCGTAAATTCTTTA+AGG + chr2.2:71461086-71461105 None:intergenic 15.0%
!! CAAACTATTTATAAATGTTA+AGG - chr2.2:71460383-71460402 MS.gene071916:intron 15.0%
!! TAGACAAATAATTATTTACA+AGG + chr2.2:71461723-71461742 None:intergenic 15.0%
!! TATTAATCTATTCTCTATTA+GGG - chr2.2:71459331-71459350 MS.gene071916:intron 15.0%
!! TTATTAATCTATTCTCTATT+AGG - chr2.2:71459330-71459349 MS.gene071916:intron 15.0%
!! TTATTTACAAATCTATAGAT+TGG + chr2.2:71461030-71461049 None:intergenic 15.0%
!! TTGATAAAAATGAGAAAAAT+CGG - chr2.2:71460605-71460624 MS.gene071916:intron 15.0%
!!! ACTTTTGTTATTATTCTTTT+TGG - chr2.2:71460256-71460275 MS.gene071916:intron 15.0%
!!! CTTTTGTTATTATTCTTTTT+GGG - chr2.2:71460257-71460276 MS.gene071916:intron 15.0%
!! AAATTTCTTCTTCAATAAGA+AGG + chr2.2:71459514-71459533 None:intergenic 20.0%
!! AATGAAAAATCAAATTGAAG+TGG + chr2.2:71459280-71459299 None:intergenic 20.0%
!! AATTTCTTCTTCAATAAGAA+GGG + chr2.2:71459513-71459532 None:intergenic 20.0%
!! GTTATTAATAATGAGTTTCT+AGG - chr2.2:71460944-71460963 MS.gene071916:intron 20.0%
!! TGTATACTTACATATGTATT+TGG - chr2.2:71460846-71460865 MS.gene071916:intron 20.0%
!! TTTGCAAATCTAATTAACAA+AGG + chr2.2:71461263-71461282 None:intergenic 20.0%
!!! ACATTTTAACTTTATCCATA+AGG - chr2.2:71461190-71461209 MS.gene071916:intron 20.0%
!!! ATAGGTTTCAATTTTAACTT+TGG - chr2.2:71459998-71460017 MS.gene071916:intron 20.0%
! AAAGATCCAACATCTAAATT+AGG - chr2.2:71460110-71460129 MS.gene071916:intron 25.0%
! AAGAGAAAAGAAAACAAAAG+GGG - chr2.2:71460577-71460596 MS.gene071916:intron 25.0%
! AATCATAAGAATAGAACAAG+AGG + chr2.2:71460412-71460431 None:intergenic 25.0%
! CAAGAATGATAATCTCTAAA+AGG + chr2.2:71460311-71460330 None:intergenic 25.0%
! CAAGAGAAAAGAAAACAAAA+GGG - chr2.2:71460576-71460595 MS.gene071916:intron 25.0%
! CATGTTATGATTAAACATTG+TGG + chr2.2:71460159-71460178 None:intergenic 25.0%
! GAGTATATCATGATTATTCT+TGG + chr2.2:71461882-71461901 None:intergenic 25.0%
! TGATATACTCAATGTTAACA+AGG - chr2.2:71461892-71461911 MS.gene071916:CDS 25.0%
! TGTAAGTATACAAATAGCAA+TGG + chr2.2:71460838-71460857 None:intergenic 25.0%
! TTAGTACACACATGTTAAAT+AGG + chr2.2:71460040-71460059 None:intergenic 25.0%
!! TTACTGTTTTACAAGTTTGT+TGG - chr2.2:71460801-71460820 MS.gene071916:intron 25.0%
AAGTTTGACAAAAAATTGCC+AGG - chr2.2:71461622-71461641 MS.gene071916:CDS 30.0%
AATGTGGAATTAGTTACCAT+CGG + chr2.2:71459928-71459947 None:intergenic 30.0%
AATTCCACTGTAATTGTTAC+AGG + chr2.2:71461447-71461466 None:intergenic 30.0%
ACATAAAGGAAAGAATCGAA+AGG - chr2.2:71461660-71461679 MS.gene071916:intron 30.0%
ACTAGATGAGAAGATAACAA+AGG + chr2.2:71461781-71461800 None:intergenic 30.0%
AGAAGCTATTCCTATTATTG+AGG - chr2.2:71459893-71459912 MS.gene071916:intron 30.0%
AGCTATCAATTACTTTGTAC+AGG - chr2.2:71460967-71460986 MS.gene071916:intron 30.0%
ATAACCAAACGTTCGATAAT+AGG + chr2.2:71459956-71459975 None:intergenic 30.0%
ATGTAGTGCTATATCGTTAA+TGG - chr2.2:71460513-71460532 MS.gene071916:intron 30.0%
CAAAACTGTTAAACAGACTA+AGG + chr2.2:71461131-71461150 None:intergenic 30.0%
CCAAGAGAAAAGAAAACAAA+AGG - chr2.2:71460575-71460594 MS.gene071916:intron 30.0%
GAAAAATCAAATTGAAGTGG+CGG + chr2.2:71459277-71459296 None:intergenic 30.0%
GCTATCAATTACTTTGTACA+GGG - chr2.2:71460968-71460987 MS.gene071916:intron 30.0%
GTAACAATTACAGTGGAATT+GGG - chr2.2:71461447-71461466 MS.gene071916:CDS 30.0%
TAACCAAACGTTCGATAATA+GGG + chr2.2:71459955-71459974 None:intergenic 30.0%
TGAAAAATGCTGCAAAGTTT+GGG - chr2.2:71460638-71460657 MS.gene071916:CDS 30.0%
TGTAACAATTACAGTGGAAT+TGG - chr2.2:71461446-71461465 MS.gene071916:CDS 30.0%
TTGAAAAATGCTGCAAAGTT+TGG - chr2.2:71460637-71460656 MS.gene071916:CDS 30.0%
TTTGTTCATGTTGTCTGATT+TGG - chr2.2:71459710-71459729 MS.gene071916:CDS 30.0%
! AAATGTCTAAAGTAGACGTA+TGG + chr2.2:71461176-71461195 None:intergenic 30.0%
! CGATTCTTTCCTTTATGTTT+TGG + chr2.2:71461658-71461677 None:intergenic 30.0%
! GTACATACATGTTTTTCTCT+GGG + chr2.2:71459491-71459510 None:intergenic 30.0%
! TGTTATGAACTTTGTTATGC+AGG - chr2.2:71459780-71459799 MS.gene071916:intron 30.0%
! TTTTCTTTTCTCTTGGCAAA+AGG + chr2.2:71460571-71460590 None:intergenic 30.0%
!! CAGAAGAAGCCTTTTAAAAA+GGG - chr2.2:71462086-71462105 MS.gene071916:CDS 30.0%
!! GTTAAGGAGTGTTTACAAAA+AGG - chr2.2:71459362-71459381 MS.gene071916:intron 30.0%
!! TCAGAAGAAGCCTTTTAAAA+AGG - chr2.2:71462085-71462104 MS.gene071916:CDS 30.0%
!! TTCTTTCCTTTATGTTTTGG+AGG + chr2.2:71461655-71461674 None:intergenic 30.0%
!!! AACCTTTATTTTTGGCAAAC+AGG + chr2.2:71459663-71459682 None:intergenic 30.0%
!!! ATGTTGTCTGATTTGGTTTT+CGG - chr2.2:71459717-71459736 MS.gene071916:CDS 30.0%
!!! CCTTTTGTTTTCTTTTCTCT+TGG + chr2.2:71460578-71460597 None:intergenic 30.0%
AAAGTCCTTAGTCTGTTCAA+AGG - chr2.2:71461354-71461373 MS.gene071916:CDS 35.0%
AACAATGCATGGATATGTCA+AGG + chr2.2:71460884-71460903 None:intergenic 35.0%
AAGGTTACCAAGGATGAATT+AGG - chr2.2:71461556-71461575 MS.gene071916:intron 35.0%
AATCCCTATTATCGAACGTT+TGG - chr2.2:71459949-71459968 MS.gene071916:intron 35.0%
AATGGTTAAGAGAGTTACCT+AGG + chr2.2:71460670-71460689 None:intergenic 35.0%
AATTGTACTCTCTGCATTCT+AGG - chr2.2:71461977-71461996 MS.gene071916:intron 35.0%
ACATTGAATTAGACCTCCTT+TGG - chr2.2:71460080-71460099 MS.gene071916:intron 35.0%
ACGGTTCCTAATTTAGATGT+TGG + chr2.2:71460119-71460138 None:intergenic 35.0%
ACTACTCTTGAAAGCTCATA+AGG + chr2.2:71460189-71460208 None:intergenic 35.0%
ACTGAAGCATAGTAACCTTA+TGG + chr2.2:71461208-71461227 None:intergenic 35.0%
ATCTTTAAACATGCCAAAGG+AGG + chr2.2:71460096-71460115 None:intergenic 35.0%
ATTCTCTATTAGGGTTGAAG+TGG - chr2.2:71459340-71459359 MS.gene071916:intron 35.0%
CAAAACCTTTGAACAGACTA+AGG + chr2.2:71461362-71461381 None:intergenic 35.0%
CAAGTTTATGATGAACCATC+TGG - chr2.2:71459828-71459847 MS.gene071916:intron 35.0%
CCTTCGATTTATCATTCCTT+CGG + chr2.2:71462037-71462056 None:intergenic 35.0%
GAAAAATGCTGCAAAGTTTG+GGG - chr2.2:71460639-71460658 MS.gene071916:CDS 35.0%
GAAAAGCCTCCAAAACATAA+AGG - chr2.2:71461646-71461665 MS.gene071916:intron 35.0%
GACCTGTTTGCCAAAAATAA+AGG - chr2.2:71459658-71459677 MS.gene071916:CDS 35.0%
GTAGGTTTAGCCTCAATAAT+AGG + chr2.2:71459906-71459925 None:intergenic 35.0%
GTGTTTATCGTTTGCTCATA+AGG - chr2.2:71459588-71459607 MS.gene071916:intron 35.0%
GTTACTATGCTTCAGTGATT+TGG - chr2.2:71461212-71461231 MS.gene071916:intron 35.0%
GTTATGCAGGCATTATTAAG+CGG - chr2.2:71459793-71459812 MS.gene071916:intron 35.0%
GTTCAACAGAAAACAATGCA+TGG + chr2.2:71460895-71460914 None:intergenic 35.0%
TACACCTGTAACAATTACAG+TGG - chr2.2:71461440-71461459 MS.gene071916:CDS 35.0%
TATGTTCAACCGCAATTAAG+TGG + chr2.2:71460995-71461014 None:intergenic 35.0%
TATTAGGGTTGAAGTGGTTA+AGG - chr2.2:71459346-71459365 MS.gene071916:intron 35.0%
TCGATAATAGGGATTGAATG+TGG + chr2.2:71459944-71459963 None:intergenic 35.0%
TGGATCTTTAAACATGCCAA+AGG + chr2.2:71460099-71460118 None:intergenic 35.0%
! AAAACCTCAAGGCTAGTTAT+TGG + chr2.2:71460694-71460713 None:intergenic 35.0%
! ATGTTTTTCTCTGGGTAATC+TGG + chr2.2:71459483-71459502 None:intergenic 35.0%
! GGTACATACATGTTTTTCTC+TGG + chr2.2:71459492-71459511 None:intergenic 35.0%
! TATAATGTTTTCAGCTGCCA+AGG - chr2.2:71459614-71459633 MS.gene071916:CDS 35.0%
! TCAAGGCTAGTTATTGGAAA+TGG + chr2.2:71460688-71460707 None:intergenic 35.0%
! TTCTTGGCACCCTTTTTAAA+AGG + chr2.2:71462098-71462117 None:intergenic 35.0%
! TTTAACTTTGGATACTTGCG+AGG - chr2.2:71460010-71460029 MS.gene071916:intron 35.0%
!! ATTTCCAATAACTAGCCTTG+AGG - chr2.2:71460687-71460706 MS.gene071916:CDS 35.0%
!! TTTAAAAAGGGTGCCAAGAA+AGG - chr2.2:71462098-71462117 MS.gene071916:CDS 35.0%
!!! GCACAGCTAACCTTTATTTT+TGG + chr2.2:71459671-71459690 None:intergenic 35.0%
AACGTTTGGTTATCGCTGTT+GGG - chr2.2:71459963-71459982 MS.gene071916:intron 40.0%
ACATACCTGGATTGTTGAGA+AGG + chr2.2:71459229-71459248 None:intergenic 40.0%
ACCACAAAAGAAGGTTACCA+AGG - chr2.2:71461546-71461565 MS.gene071916:intron 40.0%
ACCTTCAACATCTTGAGTAG+AGG + chr2.2:71459432-71459451 None:intergenic 40.0%
AGGCATTATTAAGCGGAACA+AGG - chr2.2:71459800-71459819 MS.gene071916:intron 40.0%
ATGCATGGATATGTCAAGGT+AGG + chr2.2:71460880-71460899 None:intergenic 40.0%
CATACCTGGATTGTTGAGAA+GGG + chr2.2:71459228-71459247 None:intergenic 40.0%
CCGAAGGAATGATAAATCGA+AGG - chr2.2:71462034-71462053 MS.gene071916:intron 40.0%
CCTTCAACATCTTGAGTAGA+GGG + chr2.2:71459431-71459450 None:intergenic 40.0%
GCCTAAACCTCCTACTAAAT+GGG - chr2.2:71459636-71459655 MS.gene071916:CDS 40.0%
GGAGTTTGTACATAGTTCAG+AGG + chr2.2:71461110-71461129 None:intergenic 40.0%
GTATGAATGATGAGAGTCGA+AGG + chr2.2:71459206-71459225 None:intergenic 40.0%
TACACTGCCTAATTCATCCT+TGG + chr2.2:71461566-71461585 None:intergenic 40.0%
TCAGTCAGGACTTCAAAACT+TGG - chr2.2:71460225-71460244 MS.gene071916:intron 40.0%
TCCCATTTAGTAGGAGGTTT+AGG + chr2.2:71459640-71459659 None:intergenic 40.0%
TGCATGGATATGTCAAGGTA+GGG + chr2.2:71460879-71460898 None:intergenic 40.0%
TGGAATTAGTTACCATCGGT+AGG + chr2.2:71459924-71459943 None:intergenic 40.0%
TGTTATAGGCAAAGAAGTCG+CGG + chr2.2:71461515-71461534 None:intergenic 40.0%
! AATTTGTTCGAGGTTGACCT+CGG - chr2.2:71459169-71459188 MS.gene071916:CDS 40.0%
! GCTTTTCTCCTTCTTCACAT+TGG + chr2.2:71462015-71462034 None:intergenic 40.0%
! TTTTTCTCTGGGTAATCTGG+TGG + chr2.2:71459480-71459499 None:intergenic 40.0%
!! GGACGATCTGATGCTTTTTA+CGG + chr2.2:71460138-71460157 None:intergenic 40.0%
!! TCCTTGGTAACCTTCTTTTG+TGG + chr2.2:71461550-71461569 None:intergenic 40.0%
!!! AGTTTTGAAGTCCTGACTGA+TGG + chr2.2:71460225-71460244 None:intergenic 40.0%
!!! GTTTTGAAGTCCTGACTGAT+GGG + chr2.2:71460224-71460243 None:intergenic 40.0%
AACAGGTCCCATTTAGTAGG+AGG + chr2.2:71459646-71459665 None:intergenic 45.0%
ACCATGCAACGAAAACCTCA+AGG + chr2.2:71460705-71460724 None:intergenic 45.0%
ACGTTTGGTTATCGCTGTTG+GGG - chr2.2:71459964-71459983 MS.gene071916:intron 45.0%
ATACATCATCTGCTCGTGCT+TGG - chr2.2:71460750-71460769 MS.gene071916:intron 45.0%
ATATCCATGTCTGCGTTTCC+AGG + chr2.2:71459856-71459875 None:intergenic 45.0%
ATTGAGGCTAAACCTACCGA+TGG - chr2.2:71459909-71459928 MS.gene071916:intron 45.0%
CACAGTGACCAATGTGAAGA+AGG - chr2.2:71462004-71462023 MS.gene071916:intron 45.0%
CACGAGCAGATGATGTATAG+TGG + chr2.2:71460748-71460767 None:intergenic 45.0%
CATACCCTTCTCAACAATCC+AGG - chr2.2:71459221-71459240 MS.gene071916:CDS 45.0%
CCATGATTTACCCATCAGTC+AGG - chr2.2:71460211-71460230 MS.gene071916:intron 45.0%
CCCTCTACTCAAGATGTTGA+AGG - chr2.2:71459428-71459447 MS.gene071916:CDS 45.0%
GAACGTTTGGTTATCGCTGT+TGG - chr2.2:71459962-71459981 MS.gene071916:intron 45.0%
GATGAATTAGGCAGTGTAGC+AGG - chr2.2:71461568-71461587 MS.gene071916:intron 45.0%
GCAAACAGGTCCCATTTAGT+AGG + chr2.2:71459649-71459668 None:intergenic 45.0%
GCGACTTCTTTGCCTATAAC+AGG - chr2.2:71461514-71461533 MS.gene071916:intron 45.0%
GGCCTAAACCTCCTACTAAA+TGG - chr2.2:71459635-71459654 MS.gene071916:CDS 45.0%
GGTTGACCTCGGTAATCTAA+TGG - chr2.2:71459180-71459199 MS.gene071916:CDS 45.0%
GTACAGGGTCCACTTAATTG+CGG - chr2.2:71460983-71461002 MS.gene071916:intron 45.0%
GTTGGGGACAACTAGGAAAT+AGG - chr2.2:71459980-71459999 MS.gene071916:intron 45.0%
TGAAGAAGGAGAAAAGCCGA+AGG - chr2.2:71462018-71462037 MS.gene071916:intron 45.0%
TTAGTAGGAGGTTTAGGCCT+TGG + chr2.2:71459634-71459653 None:intergenic 45.0%
! AACCTTCTTTTGTGGTGCGT+GGG + chr2.2:71461542-71461561 None:intergenic 45.0%
! ACATTTTGCAGATCCAGACG+AGG - chr2.2:71460543-71460562 MS.gene071916:intron 45.0%
! AGAAAGGAGGTGTACATACC+TGG + chr2.2:71459242-71459261 None:intergenic 45.0%
! ATGATGAACCATCTGGAACC+TGG - chr2.2:71459835-71459854 MS.gene071916:intron 45.0%
! CAAAGTTTGGGGCTTTACCT+AGG - chr2.2:71460650-71460669 MS.gene071916:CDS 45.0%
! CCTGACTGATGGGTAAATCA+TGG + chr2.2:71460214-71460233 None:intergenic 45.0%
! GGCTGCTAAGAATTTGTTCG+AGG - chr2.2:71459159-71459178 MS.gene071916:CDS 45.0%
! TAACCTTCTTTTGTGGTGCG+TGG + chr2.2:71461543-71461562 None:intergenic 45.0%
! TCTTCTCATCTAGTTCCTGC+CGG - chr2.2:71461787-71461806 MS.gene071916:CDS 45.0%
!!! TGCTTTTGGGTTGCCTTTCT+TGG + chr2.2:71462114-71462133 None:intergenic 45.0%
!!! TGTTTTGGAGGCTTTTCACC+TGG + chr2.2:71461643-71461662 None:intergenic 45.0%
AAACTTCCCACCACTAGCTG+TGG + chr2.2:71461608-71461627 None:intergenic 50.0%
AACCCACGCACCACAAAAGA+AGG - chr2.2:71461537-71461556 MS.gene071916:intron 50.0%
ACCATGCAGCCATGTTCAGT+TGG - chr2.2:71461489-71461508 MS.gene071916:intron 50.0%
AGGAACAGGGGTAGGATCAA+AGG - chr2.2:71461817-71461836 MS.gene071916:CDS 50.0%
AGGGGTAGGATCAAAGGAGA+GGG - chr2.2:71461823-71461842 MS.gene071916:CDS 50.0%
CGAAGGCCATTAGATTACCG+AGG + chr2.2:71459189-71459208 None:intergenic 50.0%
TATCGCTGTTGGGGACAACT+AGG - chr2.2:71459973-71459992 MS.gene071916:intron 50.0%
TCTCTGGGTAATCTGGTGGA+TGG + chr2.2:71459476-71459495 None:intergenic 50.0%
TTGGCAAAAGGATCCTCGTC+TGG + chr2.2:71460559-71460578 None:intergenic 50.0%
! CTCTGGGTAATCTGGTGGAT+GGG + chr2.2:71459475-71459494 None:intergenic 50.0%
! GCCTTGAGGTTTTCGTTGCA+TGG - chr2.2:71460701-71460720 MS.gene071916:CDS 50.0%
! TCTGGGTAATCTGGTGGATG+GGG + chr2.2:71459474-71459493 None:intergenic 50.0%
AAGTCGCGGCCAACTGAACA+TGG + chr2.2:71461501-71461520 None:intergenic 55.0%
ACCACTAGCTGTGGCAGTTG+AGG + chr2.2:71461599-71461618 None:intergenic 55.0%
ACCCCTGTTCCTTCTGCTAC+CGG + chr2.2:71461809-71461828 None:intergenic 55.0%
ATGGGGCAGGCAATTGAACG+AGG + chr2.2:71459457-71459476 None:intergenic 55.0%
CAACTGCCACAGCTAGTGGT+GGG - chr2.2:71461599-71461618 MS.gene071916:intron 55.0%
CAGGGGTAGGATCAAAGGAG+AGG - chr2.2:71461822-71461841 MS.gene071916:CDS 55.0%
CTGCGTTTCCAGGTTCCAGA+TGG + chr2.2:71459846-71459865 None:intergenic 55.0%
GCCAACTGAACATGGCTGCA+TGG + chr2.2:71461493-71461512 None:intergenic 55.0%
GCGCTCGAAAGGAAGAAAGG+AGG + chr2.2:71459255-71459274 None:intergenic 55.0%
GGAACCTGGAAACGCAGACA+TGG - chr2.2:71459849-71459868 MS.gene071916:intron 55.0%
GGTGCGTGGGTTCCTGTTAT+AGG + chr2.2:71461529-71461548 None:intergenic 55.0%
GTAGCAGAAGGAACAGGGGT+AGG - chr2.2:71461809-71461828 MS.gene071916:CDS 55.0%
TAGTTCCTGCCGGTAGCAGA+AGG - chr2.2:71461797-71461816 MS.gene071916:CDS 55.0%
TCAACTGCCACAGCTAGTGG+TGG - chr2.2:71461598-71461617 MS.gene071916:intron 55.0%
TGCCGGTAGCAGAAGGAACA+GGG - chr2.2:71461804-71461823 MS.gene071916:CDS 55.0%
TGGGGCAGGCAATTGAACGA+GGG + chr2.2:71459456-71459475 None:intergenic 55.0%
CTGCCGGTAGCAGAAGGAAC+AGG - chr2.2:71461803-71461822 MS.gene071916:CDS 60.0%
CTGTTCCTTCTGCTACCGGC+AGG + chr2.2:71461805-71461824 None:intergenic 60.0%
GCCGGTAGCAGAAGGAACAG+GGG - chr2.2:71461805-71461824 MS.gene071916:CDS 60.0%
GCCTCAACTGCCACAGCTAG+TGG - chr2.2:71461595-71461614 MS.gene071916:intron 60.0%
GCGGCGCTCGAAAGGAAGAA+AGG + chr2.2:71459258-71459277 None:intergenic 60.0%
GGGGCAGGCAATTGAACGAG+GGG + chr2.2:71459455-71459474 None:intergenic 60.0%
TTGAAGTGGCGGCGCTCGAA+AGG + chr2.2:71459266-71459285 None:intergenic 60.0%
! GGTAATCTGGTGGATGGGGC+AGG + chr2.2:71459470-71459489 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 71459142 71462139 71459142 ID=MS.gene071916
chr2.2 mRNA 71459142 71462139 71459142 ID=MS.gene071916.t1;Parent=MS.gene071916
chr2.2 exon 71462039 71462139 71462039 ID=MS.gene071916.t1.exon1;Parent=MS.gene071916.t1
chr2.2 CDS 71462039 71462139 71462039 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
chr2.2 exon 71461779 71461929 71461779 ID=MS.gene071916.t1.exon2;Parent=MS.gene071916.t1
chr2.2 CDS 71461779 71461929 71461779 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
chr2.2 exon 71461602 71461653 71461602 ID=MS.gene071916.t1.exon3;Parent=MS.gene071916.t1
chr2.2 CDS 71461602 71461653 71461602 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
chr2.2 exon 71461351 71461479 71461351 ID=MS.gene071916.t1.exon4;Parent=MS.gene071916.t1
chr2.2 CDS 71461351 71461479 71461351 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
chr2.2 exon 71460610 71460727 71460610 ID=MS.gene071916.t1.exon5;Parent=MS.gene071916.t1
chr2.2 CDS 71460610 71460727 71460610 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
chr2.2 exon 71459600 71459783 71459600 ID=MS.gene071916.t1.exon6;Parent=MS.gene071916.t1
chr2.2 CDS 71459600 71459783 71459600 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
chr2.2 exon 71459368 71459481 71459368 ID=MS.gene071916.t1.exon7;Parent=MS.gene071916.t1
chr2.2 CDS 71459368 71459481 71459368 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
chr2.2 exon 71459142 71459282 71459142 ID=MS.gene071916.t1.exon8;Parent=MS.gene071916.t1
chr2.2 CDS 71459142 71459282 71459142 ID=cds.MS.gene071916.t1;Parent=MS.gene071916.t1
Gene Sequence

>MS.gene071916

ATGGAGATTGAGATGGAGGCTGCTAAGAATTTGTTCGAGGTTGACCTCGGTAATCTAATGGCCTTCGACTCTCATCATTCATACCCTTCTCAACAATCCAGGTATGTACACCTCCTTTCTTCCTTTCGAGCGCCGCCACTTCAATTTGATTTTTCATTTTCATTTTTCAATCTTTGATACATGTTCTGTTATTAATCTATTCTCTATTAGGGTTGAAGTGGTTAAGGAGTGTTTACAAAAAGGCACTGAGTTAGTTCAAGCTATCGCTGATTCTCTCTTTATTTTACCCTCTACTCAAGATGTTGAAGGTCCCCTCGTTCAATTGCCTGCCCCATCCACCAGATTACCCAGAGAAAAACATGTATGTACCCTTCTTATTGAAGAAGAAATTTTATTTATTTATTTATTTATTTATTTATAATATAAACATTTCTTTATTGATTCGTGTGTTTATCGTTTGCTCATAAGGTTTTATAATGTTTTCAGCTGCCAAGGCCTAAACCTCCTACTAAATGGGACCTGTTTGCCAAAAATAAAGGTTAGCTGTGCTGTGCTGTGCTGTGCTGCTTTTGTTCATGTTGTCTGATTTGGTTTTCGGATTAGACTTTTTTTTAACTATTTTATTACTTACTGAACTTTGTTATGAACTTTGTTATGCAGGCATTATTAAGCGGAACAAGGACAAACAAGTTTATGATGAACCATCTGGAACCTGGAAACGCAGACATGGATATGATCGTGCTAACGATGAAGAAGCTATTCCTATTATTGAGGCTAAACCTACCGATGGTAACTAATTCCACATTCAATCCCTATTATCGAACGTTTGGTTATCGCTGTTGGGGACAACTAGGAAATAGGTTTCAATTTTAACTTTGGATACTTGCGAGGCAAACCTATTTAACATGTGTGTACTAAAACAGTACAAATTAGAATAGACATTGAATTAGACCTCCTTTGGCATGTTTAAAGATCCAACATCTAAATTAGGAACCGTAAAAAGCATCAGATCGTCCACAATGTTTAATCATAACATGATAATCACCTTATGAGCTTTCAAGAGTAGTATCCATGATTTACCCATCAGTCAGGACTTCAAAACTTGGAACAAGTTACTTTTGTTATTATTCTTTTTGGGTAATTTGTCAATTTTGTATTTGATTCTGCCTTTTAGAGATTATCATTCTTGTGCGTGATAATCTAGATCAAGTACTCTGATTGAAGTTTACTTGTATTGAATTCAAACTATTTATAAATGTTAAGGGAACCTCTTGTTCTATTCTTATGATTTTTCAGAATTATTTTAACATTAAATGTTTTCATGTGCGCATACAAAATTCATACTTGAACTTTGCATTTTTACAGTTTTCTATGTAGTGCTATATCGTTAATGGTATTGTTACATTTTGCAGATCCAGACGAGGATCCTTTTGCCAAGAGAAAAGAAAACAAAAGGGGAAGAGTTGATAAAAATGAGAAAAATCGGATACAGAATTTGAAAAATGCTGCAAAGTTTGGGGCTTTACCTAGGTAACTCTCTTAACCATTTCCAATAACTAGCCTTGAGGTTTTCGTTGCATGGTTGCATTCTATTTATTTCTATCCACTATACATCATCTGCTCGTGCTTGGTGTCATTTATCTGTTGCAATCACAGTGTTTACTGTTTTACAAGTTTGTTGGAGAATTTTGATCCATTGCTATTTGTATACTTACATATGTATTTGGTCTTTTTCCCTACCTTGACATATCCATGCATTGTTTTCTGTTGAACACTTTATCTGAGCTAGTTGTATCTGCAATGTTATTAATAATGAGTTTCTAGGAGCTATCAATTACTTTGTACAGGGTCCACTTAATTGCGGTTGAACATACATTAAGCTTATCCAATCTATAGATTTGTAAATAATCACATGATTTTGTTTCTTTTTGTAATAATTCTCCTTAAAGAATTTACGATTTTTTTCCTCTGAACTATGTACAAACTCCTTAGTCTGTTTAACAGTTTTGCAGAAATACTAGTTATGAGTCGCCATACGTCTACTTTAGACATTTTAACTTTATCCATAAGGTTACTATGCTTCAGTGATTTGGAAGTGCGTAAATATGTTAATTAGTTCCTTTGTTAATTAGATTTGCAAATAATCACATGATTGTTTCTTTTGTAAGAATTCTCCATAAAGAATTTTCAATTTTTTCTCTGAACAATGTGAAAAGTCCTTAGTCTGTTCAAAGGTTTTGTATATATCTACTGTTGTGTAACTAGTTGTGACGTCACTCTGATTTCTTTGCATTCTTTTACACCTGTAACAATTACAGTGGAATTGGGCTCTAATTCTAAACAATTTACCATGCAGCCATGTTCAGTTGGCCGCGACTTCTTTGCCTATAACAGGAACCCACGCACCACAAAAGAAGGTTACCAAGGATGAATTAGGCAGTGTAGCAGGTTTTGCCTCAACTGCCACAGCTAGTGGTGGGAAGTTTGACAAAAAATTGCCAGGTGAAAAGCCTCCAAAACATAAAGGAAAGAATCGAAAGGTGTGTACTGTACTCTTACAGAAATCAGTTTATCTTAACCTTGTAAATAATTATTTGTCTAAACAGTTAACTTTCATTTAATTGACTGATTAGCTTCCTTTGTTATCTTCTCATCTAGTTCCTGCCGGTAGCAGAAGGAACAGGGGTAGGATCAAAGGAGAGGGAGCAAACTGAAAATATATTGAACAAGATCATGTCCAAGAATAATCATGATATACTCAATGTTAACAAGGTACATTCAAGTTATTAAGTAACTAGTGACATTGATTTCAATTGTTATATTTTTTCTTTTCATAATTGTACTCTCTGCATTCTAGGCTGTCACAGTGACCAATGTGAAGAAGGAGAAAAGCCGAAGGAATGATAAATCGAAGGCTTCAGCAACTAACAGCAAATTGAAAACTCAGAAGAAGCCTTTTAAAAAGGGTGCCAAGAAAGGCAACCCAAAAGCACAGTAA
Protein sequence

>MS.gene071916.t1

MEIEMEAAKNLFEVDLGNLMAFDSHHSYPSQQSRVEVVKECLQKGTELVQAIADSLFILPSTQDVEGPLVQLPAPSTRLPREKHLPRPKPPTKWDLFAKNKGIIKRNKDKQVYDEPSGTWKRRHGYDRANDEEAIPIIEAKPTDDPDEDPFAKRKENKRGRVDKNEKNRIQNLKNAAKFGALPSHVQLAATSLPITGTHAPQKKVTKDELGSVAGFASTATASGGKFDKKLPGEKPPKHKGKNRKFLPVAEGTGVGSKEREQTENILNKIMSKNNHDILNVNKAVTVTNVKKEKSRRNDKSKASATNSKLKTQKKPFKKGAKKGNPKAQ