AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01172

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01172.t1 MTR_2g008870 93.958 331 18 2 1 329 1 331 0.0 625
MS.gene01172.t1 MTR_2g008870 94.718 284 14 1 1 283 1 284 0.0 551
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01172.t1 AT2G37990 60.308 325 120 3 1 324 1 317 3.47e-130 374

Find 74 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAAAATTACTGTGCTTT+TGG 0.169446 2.1:+73909277 None:intergenic
GGCCAAAACCTCCTACTAAA+TGG 0.206025 2.1:-73912063 MS.gene01172:CDS
CGATTCTTTCCTTTATGTTT+TGG 0.214740 2.1:+73909765 None:intergenic
TCCCATTTAGTAGGAGGTTT+TGG 0.249068 2.1:+73912061 None:intergenic
GAGTATATCATGATTATTCT+TGG 0.294298 2.1:+73909541 None:intergenic
CTCGGGGTAATCTGGTGGTT+GGG 0.302345 2.1:+73912210 None:intergenic
GTGAAAGAGTGTTTACAAAA+AGG 0.307046 2.1:-73912320 MS.gene01172:CDS
GGTGCGTGGGTTCCTGTTAT+AGG 0.317411 2.1:+73909894 None:intergenic
GTAGGTTTAGCCTCAATAAT+AGG 0.359792 2.1:+73911812 None:intergenic
ATATCCATGTCTGCGTTTCC+AGG 0.400440 2.1:+73911862 None:intergenic
CCTTCGATTTATCATTCCTT+CGG 0.407735 2.1:+73909368 None:intergenic
GCGACATCTTTGCCTATAAC+AGG 0.413812 2.1:-73909906 MS.gene01172:CDS
CCCTCTACTCAAGATGTTGA+AGG 0.416054 2.1:-73912254 MS.gene01172:CDS
GCAAACAGGTCCCATTTAGT+AGG 0.428944 2.1:+73912052 None:intergenic
TCTCGGGGTAATCTGGTGGT+TGG 0.429457 2.1:+73912209 None:intergenic
CTACCGGTAGCAGAAGGAAC+AGG 0.433565 2.1:-73909617 MS.gene01172:CDS
GGTTGACCTCGGTAATCTAA+TGG 0.449578 2.1:-73912489 MS.gene01172:CDS
CAAGTTTATGATGAACCATC+TGG 0.452578 2.1:-73911887 MS.gene01172:CDS
GCCAAAACCTCCTACTAAAT+GGG 0.459534 2.1:-73912062 MS.gene01172:CDS
GAAAAGCCTCCAAAACATAA+AGG 0.462069 2.1:-73909774 MS.gene01172:CDS
TCGGGGTAATCTGGTGGTTG+GGG 0.468261 2.1:+73912211 None:intergenic
TTGGCAAAAGGATCCTCGTC+TGG 0.469582 2.1:+73911101 None:intergenic
ACCCCTGTTCCTTCTGCTAC+CGG 0.474967 2.1:+73909614 None:intergenic
AGGAACAGGGGTAGGATCAA+AGG 0.493785 2.1:-73909603 MS.gene01172:CDS
GCCTCAACTGCCACAGCTAG+TGG 0.494544 2.1:-73909825 MS.gene01172:CDS
GGCTGCTAAGAATTTGTTCG+AGG 0.502076 2.1:-73912510 MS.gene01172:CDS
AAGGTTACCAAGGATGAATT+AGG 0.504736 2.1:-73909864 MS.gene01172:CDS
CACAGTGACCAATGTGAAGA+AGG 0.507110 2.1:-73909398 MS.gene01172:CDS
CTGCGTTTCCAGGTTCCAGA+TGG 0.508540 2.1:+73911872 None:intergenic
TACACTGCCTAATTCATCCT+TGG 0.511559 2.1:+73909857 None:intergenic
GATGAATTAGGCAGTGTAGC+AGG 0.517015 2.1:-73909852 MS.gene01172:CDS
AAGAGAAAAGAAAACAAAAG+AGG 0.517890 2.1:-73911080 MS.gene01172:CDS
ATGATGAACCATCTGGAACC+TGG 0.518041 2.1:-73911880 MS.gene01172:CDS
AACCCACGCACCACAAAAGA+AGG 0.519084 2.1:-73909883 MS.gene01172:CDS
AAAGAAAATGGAGATTGAGA+TGG 0.520851 2.1:-73912534 None:intergenic
AGAAGCTATTCCTATTATTG+AGG 0.529821 2.1:-73911822 MS.gene01172:CDS
CAAAGTTTGGGGCTTTACCT+AGG 0.530560 2.1:-73911007 MS.gene01172:intron
GAAAATGGAGATTGAGATGG+AGG 0.531102 2.1:-73912531 None:intergenic
ACCACTAGCTGTGGCAGTTG+AGG 0.531694 2.1:+73909824 None:intergenic
CATACCCTTCTCAACAATCC+AGG 0.537224 2.1:-73912448 MS.gene01172:intron
AACAGGTCCCATTTAGTAGG+AGG 0.542260 2.1:+73912055 None:intergenic
ACCATGCAGCCATGTTCAGT+TGG 0.543010 2.1:-73909931 MS.gene01172:intron
ACATACCTGGATTGTTGAGA+AGG 0.562948 2.1:+73912443 None:intergenic
CCTTCAACATCTTGAGTAGA+GGG 0.564606 2.1:+73912254 None:intergenic
ATGTCGCGGCCAACTGAACA+TGG 0.570065 2.1:+73909922 None:intergenic
TCAACTGCCACAGCTAGTGG+TGG 0.576419 2.1:-73909822 MS.gene01172:CDS
ACCTTCAACATCTTGAGTAG+AGG 0.587465 2.1:+73912253 None:intergenic
TGGAATTACTTACCATCGGT+AGG 0.591410 2.1:+73911794 None:intergenic
CATACCTGGATTGTTGAGAA+GGG 0.605884 2.1:+73912444 None:intergenic
GGAACCTGGAAACGCAGACA+TGG 0.610484 2.1:-73911866 MS.gene01172:CDS
TACCGGTAGCAGAAGGAACA+GGG 0.611779 2.1:-73909616 MS.gene01172:CDS
AGGGGTAGGATCAAAGGAGA+GGG 0.618168 2.1:-73909597 MS.gene01172:CDS
AGGCATTATTAAGCGGAACA+AGG 0.619070 2.1:-73911915 MS.gene01172:CDS
CAACTGCCACAGCTAGTGGT+GGG 0.621815 2.1:-73909821 MS.gene01172:CDS
TAGTTCCTACCGGTAGCAGA+AGG 0.622035 2.1:-73909623 MS.gene01172:CDS
GTTATGCAGGCATTATTAAG+CGG 0.622663 2.1:-73911922 MS.gene01172:intron
CTGTTCCTTCTGCTACCGGT+AGG 0.633674 2.1:+73909618 None:intergenic
CCGAAGGAATGATAAATCGA+AGG 0.634190 2.1:-73909368 MS.gene01172:CDS
AAACTTCCCACCACTAGCTG+TGG 0.634673 2.1:+73909815 None:intergenic
GTATGAATGATGAGAGTCGA+AGG 0.637332 2.1:+73912466 None:intergenic
AATTTGTTCGAGGTTGACCT+CGG 0.638182 2.1:-73912500 MS.gene01172:CDS
GTAGCAGAAGGAACAGGGGT+AGG 0.639483 2.1:-73909611 MS.gene01172:CDS
TGATATACTCAATGTTAACA+AGG 0.643030 2.1:-73909528 MS.gene01172:intron
CAGGGGTAGGATCAAAGGAG+AGG 0.644223 2.1:-73909598 MS.gene01172:CDS
ATTGAGGCTAAACCTACCGA+TGG 0.658675 2.1:-73911806 MS.gene01172:intron
TGTTATAGGCAAAGATGTCG+CGG 0.661140 2.1:+73909908 None:intergenic
TGAAGAAGGAGAAAAGCCGA+AGG 0.662135 2.1:-73909384 MS.gene01172:CDS
ACATAAAGGAAAGAATCGAA+AGG 0.670337 2.1:-73909760 MS.gene01172:intron
ACCACAAAAGAAGGTTACCA+AGG 0.679267 2.1:-73909874 MS.gene01172:CDS
GGGGCAGACAATTGAACGAG+GGG 0.691274 2.1:+73912230 None:intergenic
TGGGGCAGACAATTGAACGA+GGG 0.704031 2.1:+73912229 None:intergenic
CGAAGGCCATTAGATTACCG+AGG 0.720268 2.1:+73912483 None:intergenic
TTGGGGCAGACAATTGAACG+AGG 0.741722 2.1:+73912228 None:intergenic
ACCGGTAGCAGAAGGAACAG+GGG 0.747731 2.1:-73909615 MS.gene01172:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTGTAAATTCTTTA+TGG + chr2.1:73911278-73911297 None:intergenic 10.0%
!! AAACTATTTATAAATGTTAA+GGG - chr2.1:73910539-73910558 MS.gene01172:intron 10.0%
!! CAAACTATTTATAAATGTTA+AGG - chr2.1:73910538-73910557 MS.gene01172:intron 15.0%
!! TATTAATCTATTCTCTATTA+GGG - chr2.1:73909461-73909480 MS.gene01172:intron 15.0%
!! TTATTAATCTATTCTCTATT+AGG - chr2.1:73909460-73909479 MS.gene01172:intron 15.0%
!! TTATTTACAAATCTATAGAT+TGG + chr2.1:73911222-73911241 None:intergenic 15.0%
!! TTGATAAAAATGAGAAAAAT+CGG - chr2.1:73910760-73910779 MS.gene01172:intron 15.0%
!!! ACATTTTAACTTTATCTATA+AGG - chr2.1:73911381-73911400 MS.gene01172:intron 15.0%
!!! ACTTTTGTTATTATTCTTTT+TGG - chr2.1:73910420-73910439 MS.gene01172:intron 15.0%
!!! CTTTTGTTATTATTCTTTTT+GGG - chr2.1:73910421-73910440 MS.gene01172:intron 15.0%
!! AAATTTCTTCTTCAATAAGA+AGG + chr2.1:73909644-73909663 None:intergenic 20.0%
!! AATTTCTTCTTCAATAAGAA+GGG + chr2.1:73909643-73909662 None:intergenic 20.0%
!! ATTGAAAAATCAAATTGAAG+TGG + chr2.1:73909423-73909442 None:intergenic 20.0%
!! GTTATTAATAATGAGTTTCT+AGG - chr2.1:73911099-73911118 MS.gene01172:CDS 20.0%
!! TCGACAAATAATTATTTACA+AGG + chr2.1:73912115-73912134 None:intergenic 20.0%
!! TGTATACTTACATATGTATT+TGG - chr2.1:73911001-73911020 MS.gene01172:intron 20.0%
!! TTTGCAAATCTAATTAACAA+AGG + chr2.1:73911454-73911473 None:intergenic 20.0%
!!! ACATTTTAACTTTATCCATA+AGG - chr2.1:73911623-73911642 MS.gene01172:intron 20.0%
!!! ATACGTTTCAATTTTAACTT+TGG - chr2.1:73910162-73910181 MS.gene01172:intron 20.0%
! AAAGATCCAACATCTAAATT+AGG - chr2.1:73910274-73910293 MS.gene01172:intron 25.0%
! AAGAGAAAAGAAAACAAAAG+AGG - chr2.1:73910732-73910751 MS.gene01172:intron 25.0%
! AATCATAAGAATAGAACAAG+AGG + chr2.1:73910567-73910586 None:intergenic 25.0%
! ACATAAACAACATCAACAAA+AGG + chr2.1:73909807-73909826 None:intergenic 25.0%
! ACTAGATGAAAAGATAACAA+AGG + chr2.1:73912173-73912192 None:intergenic 25.0%
! AGCATAATATGAGAAAACAA+AGG + chr2.1:73911696-73911715 None:intergenic 25.0%
! GAGTATATCATGATTATTCT+TGG + chr2.1:73912274-73912293 None:intergenic 25.0%
! GATAAAGTTAAAATGTGAAG+TGG + chr2.1:73911619-73911638 None:intergenic 25.0%
! TGATATACTCAATGTTAACA+AGG - chr2.1:73912284-73912303 MS.gene01172:CDS 25.0%
! TGTAAGTATACAAATAGCAA+TGG + chr2.1:73910993-73911012 None:intergenic 25.0%
! TTAGTACACACATGTTAAAT+AGG + chr2.1:73910204-73910223 None:intergenic 25.0%
! TTGTTTATGTTGTCTGATTT+GGG - chr2.1:73909817-73909836 MS.gene01172:CDS 25.0%
!! TTACTGTTTTACAAGTTTGT+TGG - chr2.1:73910956-73910975 MS.gene01172:intron 25.0%
!!! CTGGTATTGATTTTTTTTCT+GGG - chr2.1:73910068-73910087 MS.gene01172:intron 25.0%
!!! GACAAACTAACCTTTATTTT+TGG + chr2.1:73909785-73909804 None:intergenic 25.0%
!!! TCTTTTGTTTTCTTTTCTCT+TGG + chr2.1:73910733-73910752 None:intergenic 25.0%
AAGTTTGACAAAAAATTGCC+AGG - chr2.1:73912014-73912033 MS.gene01172:intron 30.0%
AATGAAGCATAGAAACCTTA+TGG + chr2.1:73911641-73911660 None:intergenic 30.0%
AATGTGGAATTACTTACCAT+CGG + chr2.1:73910025-73910044 None:intergenic 30.0%
AATTCCACTGTAATTGTTAC+AGG + chr2.1:73911839-73911858 None:intergenic 30.0%
AATTGCAGTTTAGCCTATAT+AGG - chr2.1:73911153-73911172 MS.gene01172:intron 30.0%
ACATAAAGGAAAGAATCGAA+AGG - chr2.1:73912052-73912071 MS.gene01172:CDS 30.0%
AGAAGCTATTCCTATTATTG+AGG - chr2.1:73909990-73910009 MS.gene01172:intron 30.0%
ATGTAGTGCTATATCGTTAA+TGG - chr2.1:73910668-73910687 MS.gene01172:intron 30.0%
ATTGCAGTTTAGCCTATATA+GGG - chr2.1:73911154-73911173 MS.gene01172:intron 30.0%
CAAAACTGTTAAACAGACTA+AGG + chr2.1:73911322-73911341 None:intergenic 30.0%
CATGCTATGATTAAACATTG+TGG + chr2.1:73910323-73910342 None:intergenic 30.0%
GAAAAATCAAATTGAAGTGG+CGG + chr2.1:73909420-73909439 None:intergenic 30.0%
GTAACAATTACAGTGGAATT+GGG - chr2.1:73911839-73911858 MS.gene01172:CDS 30.0%
GTGTTTATCATTTGCTCATA+AGG - chr2.1:73909702-73909721 MS.gene01172:intron 30.0%
GTTATTATGCTTCAGTGATT+TGG - chr2.1:73911403-73911422 MS.gene01172:intron 30.0%
GTTGTTTATGTTGTCTGATT+TGG - chr2.1:73909816-73909835 MS.gene01172:CDS 30.0%
TAACCAAACATTCAAAACGA+AGG + chr2.1:73910119-73910138 None:intergenic 30.0%
TGAAAAATGCTGCAAAGTTT+GGG - chr2.1:73910793-73910812 MS.gene01172:intron 30.0%
TGTAACAATTACAGTGGAAT+TGG - chr2.1:73911838-73911857 MS.gene01172:CDS 30.0%
TTGAAAAATGCTGCAAAGTT+TGG - chr2.1:73910792-73910811 MS.gene01172:intron 30.0%
! CGATTCTTTCCTTTATGTTT+TGG + chr2.1:73912050-73912069 None:intergenic 30.0%
! TTTTCTTTTCTCTTGGCAAA+AGG + chr2.1:73910726-73910745 None:intergenic 30.0%
!! AGCTATCAATTAGTTTGTAC+AGG - chr2.1:73911122-73911141 MS.gene01172:intron 30.0%
!! CAGAAGAAGCCTTTTAAAAA+GGG - chr2.1:73912496-73912515 MS.gene01172:CDS 30.0%
!! GCTATCAATTAGTTTGTACA+GGG - chr2.1:73911123-73911142 MS.gene01172:intron 30.0%
!! GTGAAAGAGTGTTTACAAAA+AGG - chr2.1:73909492-73909511 MS.gene01172:intron 30.0%
!! TATCCTTCGTTTTGAATGTT+TGG - chr2.1:73910113-73910132 MS.gene01172:intron 30.0%
!! TCAGAAGAAGCCTTTTAAAA+AGG - chr2.1:73912495-73912514 MS.gene01172:CDS 30.0%
!! TTCTTTCCTTTATGTTTTGG+AGG + chr2.1:73912047-73912066 None:intergenic 30.0%
!!! AACCTTTATTTTTGGCAAAC+AGG + chr2.1:73909777-73909796 None:intergenic 30.0%
!!! GCTGGTATTGATTTTTTTTC+TGG - chr2.1:73910067-73910086 MS.gene01172:intron 30.0%
!!! GTATTGATTTTTTTTCTGGG+TGG - chr2.1:73910071-73910090 MS.gene01172:intron 30.0%
AAAAAAATCAATACCAGCCC+AGG + chr2.1:73910065-73910084 None:intergenic 35.0%
AAAGTCCTTAGTCTGTTCAA+AGG - chr2.1:73911545-73911564 MS.gene01172:intron 35.0%
AACAATGCATGGATATGTCA+AGG + chr2.1:73911039-73911058 None:intergenic 35.0%
AAGGTTACCAAGGATGAATT+AGG - chr2.1:73911948-73911967 MS.gene01172:intron 35.0%
AATGGTTAAGAGAGTTACCT+AGG + chr2.1:73910825-73910844 None:intergenic 35.0%
AATGTTTGGTTATCGCTGTT+GGG - chr2.1:73910127-73910146 MS.gene01172:intron 35.0%
AATTGTACTCTCTGCATTCT+AGG - chr2.1:73912387-73912406 MS.gene01172:intron 35.0%
ACACAACAGTAGATAACTGT+TGG + chr2.1:73911773-73911792 None:intergenic 35.0%
ACATTGAATTTGACCTCCTT+TGG - chr2.1:73910244-73910263 MS.gene01172:intron 35.0%
ACGGTTCCTAATTTAGATGT+TGG + chr2.1:73910283-73910302 None:intergenic 35.0%
ACTACTCTTGAAAGCTCATA+AGG + chr2.1:73910353-73910372 None:intergenic 35.0%
ATCTTTAAACATGCCAAAGG+AGG + chr2.1:73910260-73910279 None:intergenic 35.0%
ATGATAGCTTCACCCTATAT+AGG + chr2.1:73911169-73911188 None:intergenic 35.0%
CAAAACCTTTGAACAGACTA+AGG + chr2.1:73911553-73911572 None:intergenic 35.0%
CAAGTTTATGATGAACCATC+TGG - chr2.1:73909925-73909944 MS.gene01172:CDS 35.0%
CCTTCGATTTATCATTCCTT+CGG + chr2.1:73912447-73912466 None:intergenic 35.0%
GAAAAATGCTGCAAAGTTTG+GGG - chr2.1:73910794-73910813 MS.gene01172:intron 35.0%
GAAAAGCCTCCAAAACATAA+AGG - chr2.1:73912038-73912057 MS.gene01172:intron 35.0%
GACCTGTTTGCCAAAAATAA+AGG - chr2.1:73909772-73909791 MS.gene01172:CDS 35.0%
GTAGGTTTAGCCTCAATAAT+AGG + chr2.1:73910003-73910022 None:intergenic 35.0%
GTTATGCAGGCATTATTAAG+CGG - chr2.1:73909890-73909909 MS.gene01172:CDS 35.0%
GTTCAACAGAAAACAATGCA+TGG + chr2.1:73911050-73911069 None:intergenic 35.0%
TACACCTGTAACAATTACAG+TGG - chr2.1:73911832-73911851 MS.gene01172:CDS 35.0%
TAGGCTAAACTGCAATTAAG+TGG + chr2.1:73911150-73911169 None:intergenic 35.0%
TCGATAATAGGGATTGAATG+TGG + chr2.1:73910041-73910060 None:intergenic 35.0%
TGGATCTTTAAACATGCCAA+AGG + chr2.1:73910263-73910282 None:intergenic 35.0%
! AAAACCTCAAGGCTAGTTAT+TGG + chr2.1:73910849-73910868 None:intergenic 35.0%
! CTTACTGAACTTTGTTATGC+AGG - chr2.1:73909877-73909896 MS.gene01172:CDS 35.0%
! GGGTACATACATGTTTTTCT+CGG + chr2.1:73909623-73909642 None:intergenic 35.0%
! GGTACATACATGTTTTTCTC+GGG + chr2.1:73909622-73909641 None:intergenic 35.0%
! GTACATACATGTTTTTCTCG+GGG + chr2.1:73909621-73909640 None:intergenic 35.0%
! TATAATGTTTTCAGCTGCCA+AGG - chr2.1:73909728-73909747 MS.gene01172:intron 35.0%
! TCAAGGCTAGTTATTGGAAA+TGG + chr2.1:73910843-73910862 None:intergenic 35.0%
! TTCTTGGCACCCTTTTTAAA+AGG + chr2.1:73912508-73912527 None:intergenic 35.0%
! TTTAACTTTGGATACTTGCG+AGG - chr2.1:73910174-73910193 MS.gene01172:intron 35.0%
!! ATTTCCAATAACTAGCCTTG+AGG - chr2.1:73910842-73910861 MS.gene01172:intron 35.0%
!! TCTTTTCATCTAGTTCCTAC+CGG - chr2.1:73912179-73912198 MS.gene01172:intron 35.0%
!! TTTAAAAAGGGTGCCAAGAA+AGG - chr2.1:73912508-73912527 MS.gene01172:CDS 35.0%
ACATACCTGGATTGTTGAGA+AGG + chr2.1:73909372-73909391 None:intergenic 40.0%
ACCACAAAAGAAGGTTACCA+AGG - chr2.1:73911938-73911957 MS.gene01172:intron 40.0%
ACCTTCAACATCTTGAGTAG+AGG + chr2.1:73909562-73909581 None:intergenic 40.0%
AGGCATTATTAAGCGGAACA+AGG - chr2.1:73909897-73909916 MS.gene01172:CDS 40.0%
ATGCATGGATATGTCAAGGT+AGG + chr2.1:73911035-73911054 None:intergenic 40.0%
ATGTTTGGTTATCGCTGTTG+GGG - chr2.1:73910128-73910147 MS.gene01172:intron 40.0%
CAATCCCTATTATCGAACCT+GGG - chr2.1:73910045-73910064 MS.gene01172:intron 40.0%
CATACCTGGATTGTTGAGAA+GGG + chr2.1:73909371-73909390 None:intergenic 40.0%
CCGAAGGAATGATAAATCGA+AGG - chr2.1:73912444-73912463 MS.gene01172:intron 40.0%
CCTTCAACATCTTGAGTAGA+GGG + chr2.1:73909561-73909580 None:intergenic 40.0%
GAATGTTTGGTTATCGCTGT+TGG - chr2.1:73910126-73910145 MS.gene01172:intron 40.0%
GACGCGTAGTTACTCATAAT+TGG + chr2.1:73911597-73911616 None:intergenic 40.0%
GCCAAAACCTCCTACTAAAT+GGG - chr2.1:73909750-73909769 MS.gene01172:intron 40.0%
GGTGGTTGAAAAATGAGACT+TGG - chr2.1:73910089-73910108 MS.gene01172:intron 40.0%
GTATGAATGATGAGAGTCGA+AGG + chr2.1:73909349-73909368 None:intergenic 40.0%
TACACTGCCTAATTCATCCT+TGG + chr2.1:73911958-73911977 None:intergenic 40.0%
TCAATCCCTATTATCGAACC+TGG - chr2.1:73910044-73910063 MS.gene01172:intron 40.0%
TCAGTCAGGACTTCAAAACT+TGG - chr2.1:73910389-73910408 MS.gene01172:intron 40.0%
TGCATGGATATGTCAAGGTA+GGG + chr2.1:73911034-73911053 None:intergenic 40.0%
TGGAATTACTTACCATCGGT+AGG + chr2.1:73910021-73910040 None:intergenic 40.0%
TGTTATAGGCAAAGATGTCG+CGG + chr2.1:73911907-73911926 None:intergenic 40.0%
! AATTTGTTCGAGGTTGACCT+CGG - chr2.1:73909312-73909331 MS.gene01172:CDS 40.0%
! ATGTTTTTCTCGGGGTAATC+TGG + chr2.1:73909613-73909632 None:intergenic 40.0%
! GCTTTTCTCCTTCTTCACAT+TGG + chr2.1:73912425-73912444 None:intergenic 40.0%
! TCCCATTTAGTAGGAGGTTT+TGG + chr2.1:73909754-73909773 None:intergenic 40.0%
!! GGACGATCTGATGCTTTTTA+CGG + chr2.1:73910302-73910321 None:intergenic 40.0%
!! TCCTTGGTAACCTTCTTTTG+TGG + chr2.1:73911942-73911961 None:intergenic 40.0%
!!! AGTTTTGAAGTCCTGACTGA+TGG + chr2.1:73910389-73910408 None:intergenic 40.0%
!!! GTTGTCTGATTTGGGCTTTT+CGG - chr2.1:73909825-73909844 MS.gene01172:CDS 40.0%
!!! GTTTTGAAGTCCTGACTGAT+GGG + chr2.1:73910388-73910407 None:intergenic 40.0%
AACAGGTCCCATTTAGTAGG+AGG + chr2.1:73909760-73909779 None:intergenic 45.0%
ACCATGCAACGAAAACCTCA+AGG + chr2.1:73910860-73910879 None:intergenic 45.0%
ATACATCATCTGCTCGTGCT+TGG - chr2.1:73910905-73910924 MS.gene01172:intron 45.0%
ATATCCATGTCTGCGTTTCC+AGG + chr2.1:73909953-73909972 None:intergenic 45.0%
ATTGAGGCTAAACCTACCGA+TGG - chr2.1:73910006-73910025 MS.gene01172:intron 45.0%
CACAGTGACCAATGTGAAGA+AGG - chr2.1:73912414-73912433 MS.gene01172:intron 45.0%
CACGAGCAGATGATGTATAG+TGG + chr2.1:73910903-73910922 None:intergenic 45.0%
CATACCCTTCTCAACAATCC+AGG - chr2.1:73909364-73909383 MS.gene01172:CDS 45.0%
CCATGATTTACCCATCAGTC+AGG - chr2.1:73910375-73910394 MS.gene01172:intron 45.0%
CCCTCTACTCAAGATGTTGA+AGG - chr2.1:73909558-73909577 MS.gene01172:CDS 45.0%
GATGAATTAGGCAGTGTAGC+AGG - chr2.1:73911960-73911979 MS.gene01172:intron 45.0%
GCAAACAGGTCCCATTTAGT+AGG + chr2.1:73909763-73909782 None:intergenic 45.0%
GCGACATCTTTGCCTATAAC+AGG - chr2.1:73911906-73911925 MS.gene01172:CDS 45.0%
GGAGTTTGCACATAGTTCAG+AGG + chr2.1:73911301-73911320 None:intergenic 45.0%
GGCCAAAACCTCCTACTAAA+TGG - chr2.1:73909749-73909768 MS.gene01172:intron 45.0%
GGTTGACCTCGGTAATCTAA+TGG - chr2.1:73909323-73909342 MS.gene01172:CDS 45.0%
TGAAGAAGGAGAAAAGCCGA+AGG - chr2.1:73912428-73912447 MS.gene01172:intron 45.0%
! AACCTTCTTTTGTGGTGCGT+GGG + chr2.1:73911934-73911953 None:intergenic 45.0%
! ACATTTTGCAGATCCAGACG+AGG - chr2.1:73910698-73910717 MS.gene01172:intron 45.0%
! AGAAAGGAGGTGTACATACC+TGG + chr2.1:73909385-73909404 None:intergenic 45.0%
! ATGATGAACCATCTGGAACC+TGG - chr2.1:73909932-73909951 MS.gene01172:intron 45.0%
! CAAAGTTTGGGGCTTTACCT+AGG - chr2.1:73910805-73910824 MS.gene01172:intron 45.0%
! CCTGACTGATGGGTAAATCA+TGG + chr2.1:73910378-73910397 None:intergenic 45.0%
! GGCTGCTAAGAATTTGTTCG+AGG - chr2.1:73909302-73909321 MS.gene01172:CDS 45.0%
! TAACCTTCTTTTGTGGTGCG+TGG + chr2.1:73911935-73911954 None:intergenic 45.0%
! TTTTTCTCGGGGTAATCTGG+TGG + chr2.1:73909610-73909629 None:intergenic 45.0%
!! TTAGTAGGAGGTTTTGGCCT+TGG + chr2.1:73909748-73909767 None:intergenic 45.0%
!!! TGCTTTTGGGTTGCCTTTCT+TGG + chr2.1:73912524-73912543 None:intergenic 45.0%
!!! AAAAAATTGAAAATTTTTTA+TGG + chr2.1:73911510-73911529 None:intergenic 5.0%
AAACTTCCCACCACTAGCTG+TGG + chr2.1:73912000-73912019 None:intergenic 50.0%
AACCCACGCACCACAAAAGA+AGG - chr2.1:73911929-73911948 MS.gene01172:intron 50.0%
ACCAGCCCAGGTTCGATAAT+AGG + chr2.1:73910053-73910072 None:intergenic 50.0%
ACCATGCAGCCATGTTCAGT+TGG - chr2.1:73911881-73911900 MS.gene01172:CDS 50.0%
AGGAACAGGGGTAGGATCAA+AGG - chr2.1:73912209-73912228 MS.gene01172:CDS 50.0%
AGGGGTAGGATCAAAGGAGA+GGG - chr2.1:73912215-73912234 MS.gene01172:CDS 50.0%
CCAGCCCAGGTTCGATAATA+GGG + chr2.1:73910052-73910071 None:intergenic 50.0%
CGAAGGCCATTAGATTACCG+AGG + chr2.1:73909332-73909351 None:intergenic 50.0%
TACCGGTAGCAGAAGGAACA+GGG - chr2.1:73912196-73912215 MS.gene01172:intron 50.0%
TAGTTCCTACCGGTAGCAGA+AGG - chr2.1:73912189-73912208 MS.gene01172:intron 50.0%
TATCGCTGTTGGGGACAACT+AGG - chr2.1:73910137-73910156 MS.gene01172:intron 50.0%
TGGGGCAGACAATTGAACGA+GGG + chr2.1:73909586-73909605 None:intergenic 50.0%
TTGGCAAAAGGATCCTCGTC+TGG + chr2.1:73910714-73910733 None:intergenic 50.0%
TTGGGGCAGACAATTGAACG+AGG + chr2.1:73909587-73909606 None:intergenic 50.0%
! GCCTTGAGGTTTTCGTTGCA+TGG - chr2.1:73910856-73910875 MS.gene01172:intron 50.0%
!!! TGTTTTGGAGGCTTTTCGCC+TGG + chr2.1:73912035-73912054 None:intergenic 50.0%
ACCACTAGCTGTGGCAGTTG+AGG + chr2.1:73911991-73912010 None:intergenic 55.0%
ACCCCTGTTCCTTCTGCTAC+CGG + chr2.1:73912201-73912220 None:intergenic 55.0%
ACCGGTAGCAGAAGGAACAG+GGG - chr2.1:73912197-73912216 MS.gene01172:intron 55.0%
ATGTCGCGGCCAACTGAACA+TGG + chr2.1:73911893-73911912 None:intergenic 55.0%
CAACTGCCACAGCTAGTGGT+GGG - chr2.1:73911991-73912010 MS.gene01172:intron 55.0%
CAGGGGTAGGATCAAAGGAG+AGG - chr2.1:73912214-73912233 MS.gene01172:CDS 55.0%
CCCTATTATCGAACCTGGGC+TGG - chr2.1:73910049-73910068 MS.gene01172:intron 55.0%
CTACCGGTAGCAGAAGGAAC+AGG - chr2.1:73912195-73912214 MS.gene01172:intron 55.0%
CTCGGGGTAATCTGGTGGTT+GGG + chr2.1:73909605-73909624 None:intergenic 55.0%
CTGCGTTTCCAGGTTCCAGA+TGG + chr2.1:73909943-73909962 None:intergenic 55.0%
CTGTTCCTTCTGCTACCGGT+AGG + chr2.1:73912197-73912216 None:intergenic 55.0%
GCCAACTGAACATGGCTGCA+TGG + chr2.1:73911885-73911904 None:intergenic 55.0%
GCGCTCGAAAGGAAGAAAGG+AGG + chr2.1:73909398-73909417 None:intergenic 55.0%
GGAACCTGGAAACGCAGACA+TGG - chr2.1:73909946-73909965 MS.gene01172:intron 55.0%
GGGGCAGACAATTGAACGAG+GGG + chr2.1:73909585-73909604 None:intergenic 55.0%
GGTGCGTGGGTTCCTGTTAT+AGG + chr2.1:73911921-73911940 None:intergenic 55.0%
GTAGCAGAAGGAACAGGGGT+AGG - chr2.1:73912201-73912220 MS.gene01172:intron 55.0%
TCAACTGCCACAGCTAGTGG+TGG - chr2.1:73911990-73912009 MS.gene01172:intron 55.0%
TCGGGGTAATCTGGTGGTTG+GGG + chr2.1:73909604-73909623 None:intergenic 55.0%
TCTCGGGGTAATCTGGTGGT+TGG + chr2.1:73909606-73909625 None:intergenic 55.0%
GCCTCAACTGCCACAGCTAG+TGG - chr2.1:73911987-73912006 MS.gene01172:intron 60.0%
GCGGCGCTCGAAAGGAAGAA+AGG + chr2.1:73909401-73909420 None:intergenic 60.0%
TTGAAGTGGCGGCGCTCGAA+AGG + chr2.1:73909409-73909428 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 73909285 73912549 73909285 ID=MS.gene01172
chr2.1 mRNA 73909285 73912549 73909285 ID=MS.gene01172.t1;Parent=MS.gene01172
chr2.1 exon 73912449 73912549 73912449 ID=MS.gene01172.t1.exon1;Parent=MS.gene01172.t1
chr2.1 CDS 73912449 73912549 73912449 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
chr2.1 exon 73912202 73912352 73912202 ID=MS.gene01172.t1.exon2;Parent=MS.gene01172.t1
chr2.1 CDS 73912202 73912352 73912202 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
chr2.1 exon 73912041 73912092 73912041 ID=MS.gene01172.t1.exon3;Parent=MS.gene01172.t1
chr2.1 CDS 73912041 73912092 73912041 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
chr2.1 exon 73911807 73911935 73911807 ID=MS.gene01172.t1.exon4;Parent=MS.gene01172.t1
chr2.1 CDS 73911807 73911935 73911807 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
chr2.1 exon 73911008 73911125 73911008 ID=MS.gene01172.t1.exon5;Parent=MS.gene01172.t1
chr2.1 CDS 73911008 73911125 73911008 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
chr2.1 exon 73909761 73909944 73909761 ID=MS.gene01172.t1.exon6;Parent=MS.gene01172.t1
chr2.1 CDS 73909761 73909944 73909761 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
chr2.1 exon 73909529 73909642 73909529 ID=MS.gene01172.t1.exon7;Parent=MS.gene01172.t1
chr2.1 CDS 73909529 73909642 73909529 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
chr2.1 exon 73909285 73909425 73909285 ID=MS.gene01172.t1.exon8;Parent=MS.gene01172.t1
chr2.1 CDS 73909285 73909425 73909285 ID=cds.MS.gene01172.t1;Parent=MS.gene01172.t1
Gene Sequence

>MS.gene01172

ATGGAGATTGAGATGGAGGCTGCTAAGAATTTGTTCGAGGTTGACCTCGGTAATCTAATGGCCTTCGACTCTCATCATTCATACCCTTCTCAACAATCCAGGTATGTACACCTCCTTTCTTCCTTTCGAGCGCCGCCACTTCAATTTGATTTTTCAATCTTTGATACATGTTCTGTTATTAATCTATTCTCTATTAGGGTTGAAGTTGTGAAAGAGTGTTTACAAAAAGGCACTGAGTTAGTTCAAGCTATCGCTGATTCTCTCTTTATTTTACCCTCTACTCAAGATGTTGAAGGTCCCCTCGTTCAATTGTCTGCCCCAACCACCAGATTACCCCGAGAAAAACATGTATGTACCCTTCTTATTGAAGAAGAAATTTTATTTATTTATAATATAAACATTTCTTTATTGATTCGTGTGTTTATCATTTGCTCATAAGGTTTTATAATGTTTTCAGCTGCCAAGGCCAAAACCTCCTACTAAATGGGACCTGTTTGCCAAAAATAAAGGTTAGTTTGTCCTTTTGTTGATGTTGTTTATGTTGTCTGATTTGGGCTTTTCGGATTAGACTTTTTTTTTAACTATTTTATTACTTACTGAACTTTGTTATGCAGGCATTATTAAGCGGAACAAGGACAAACAAGTTTATGATGAACCATCTGGAACCTGGAAACGCAGACATGGATATGATCGTGCTAACGATGAAGAAGCTATTCCTATTATTGAGGCTAAACCTACCGATGGTAAGTAATTCCACATTCAATCCCTATTATCGAACCTGGGCTGGTATTGATTTTTTTTCTGGGTGGTTGAAAAATGAGACTTGGATATCCTTCGTTTTGAATGTTTGGTTATCGCTGTTGGGGACAACTAGGAAATACGTTTCAATTTTAACTTTGGATACTTGCGAGGCAAACCTATTTAACATGTGTGTACTAAAACAGTACAAATTAGAATAGACATTGAATTTGACCTCCTTTGGCATGTTTAAAGATCCAACATCTAAATTAGGAACCGTAAAAAGCATCAGATCGTCCACAATGTTTAATCATAGCATGATAATCACCTTATGAGCTTTCAAGAGTAGTATCCATGATTTACCCATCAGTCAGGACTTCAAAACTTGGAATAAGTTACTTTTGTTATTATTCTTTTTGGGTAATTTGTCAATTTTGTATTTGATTCTGCATTTTTGAGATTATCATTCTTGTGCTAGATCAAGTACTCTGATTGAAGTTTACTTGTATTGAATTCAAACTATTTATAAATGTTAAGGGAACCTCTTGTTCTATTCTTATGATTTTTCAGAATTATTTTAACATTAAATGTTTTCATGTGCGCATACAAAATTCATACTTGAACTTTGCATTTTTACAGTTTTCTATGTAGTGCTATATCGTTAATGGTATTGTTACATTTTGCAGATCCAGACGAGGATCCTTTTGCCAAGAGAAAAGAAAACAAAAGAGGAAGAGTTGATAAAAATGAGAAAAATCGGATACAGAATTTGAAAAATGCTGCAAAGTTTGGGGCTTTACCTAGGTAACTCTCTTAACCATTTCCAATAACTAGCCTTGAGGTTTTCGTTGCATGGTTGCATTCTATTTATTTCTATCCACTATACATCATCTGCTCGTGCTTGGTGTCATTTATCTGTTGCAATCATAGTGTTTACTGTTTTACAAGTTTGTTGGAGAATTTTGATCCATTGCTATTTGTATACTTACATATGTATTTGGTCTTTTTCCCTACCTTGACATATCCATGCATTGTTTTCTGTTGAACACTTTATCTGAGCTAGTTGTATCTGCAATGTTATTAATAATGAGTTTCTAGGAGCTATCAATTAGTTTGTACAGGGTCCACTTAATTGCAGTTTAGCCTATATAGGGTGAAGCTATCATAAATTGCAGTTGAACATACATTAAGCAACTCCAATCTATAGATTTGTAAATAATCACATGATTTTGTTTCTTTTTGTAATAATTCTCCATAAAGAATTTACAATTTTTTCCTCTGAACTATGTGCAAACTCCTTAGTCTGTTTAACAGTTTTGCAGAAATACTAGTTATGAGTCGCTATACGTCTACTTTAGACATTTTAACTTTATCTATAAGGTTATTATGCTTCAGTGATTTGGAAGTGCGTAAATATGTTTATTAGTTCCTTTGTTAATTAGATTTGCAAATAATCACATGATTGTTTCTTTTGTAAGAATTCTCCATAAAAAATTTTCAATTTTTTCTCTGAACAATGCGAAAAGTCCTTAGTCTGTTCAAAGGTTTTGTATATATCAATGCTAGAAATACCAATTATGAGTAACTACGCGTCCACTTCACATTTTAACTTTATCCATAAGGTTTCTATGCTTCATTAATTCGCAAGTGCATAACTATGTTAATTAGATCCTTTGTTTTCTCATATTATGCTGCAAACAGTCACAGAAGAAGTTGAAAAGAACTGTGTTAATCGTCTAAATAACTACCAACAGTTATCTACTGTTGTGTAACTAGCTGTGACATCACTCTGATTTCTTTGCATTCTTTTACACCTGTAACAATTACAGTGGAATTGGGCTCTAATTCTAAACAATTTACCATGCAGCCATGTTCAGTTGGCCGCGACATCTTTGCCTATAACAGGAACCCACGCACCACAAAAGAAGGTTACCAAGGATGAATTAGGCAGTGTAGCAGGCTTTGCCTCAACTGCCACAGCTAGTGGTGGGAAGTTTGACAAAAAATTGCCAGGCGAAAAGCCTCCAAAACATAAAGGAAAGAATCGAAAGGTGTGAACTGCACTCTTACAGAAATCAGTTTATCTTAACCTTGTAAATAATTATTTGTCGAAACAGTTAACTTTCATTTAATTGACTGATTAGCTTCCTTTGTTATCTTTTCATCTAGTTCCTACCGGTAGCAGAAGGAACAGGGGTAGGATCAAAGGAGAGGGAGCAAACTGAAAATATATTGAACAAGATCATGTCCAAGAATAATCATGATATACTCAATGTTAACAAGGTACATTCAAGTTATTAAGTAACTAGTGACATTGATTTCAATTGTTATAAAGTTTTACTAGCTGTTATTTTTTCTTTTCATAATTGTACTCTCTGCATTCTAGGCTGTCACAGTGACCAATGTGAAGAAGGAGAAAAGCCGAAGGAATGATAAATCGAAGGCTTCAGCAACTAACAGCAAATTGAAAACTCAGAAGAAGCCTTTTAAAAAGGGTGCCAAGAAAGGCAACCCAAAAGCACAGTAA
Protein sequence

>MS.gene01172.t1

MEIEMEAAKNLFEVDLGNLMAFDSHHSYPSQQSRVEVVKECLQKGTELVQAIADSLFILPSTQDVEGPLVQLSAPTTRLPREKHLPRPKPPTKWDLFAKNKGIIKRNKDKQVYDEPSGTWKRRHGYDRANDEEAIPIIEAKPTDDPDEDPFAKRKENKRGRVDKNEKNRIQNLKNAAKFGALPSHVQLAATSLPITGTHAPQKKVTKDELGSVAGFASTATASGGKFDKKLPGEKPPKHKGKNRKFLPVAEGTGVGSKEREQTENILNKIMSKNNHDILNVNKAVTVTNVKKEKSRRNDKSKASATNSKLKTQKKPFKKGAKKGNPKAQ