AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99541

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99541.t1 MTR_8g046140 98.343 181 3 0 14 194 1 181 2.27e-131 366
MS.gene99541.t1 MTR_3g007700 98.333 180 3 0 15 194 3 182 1.36e-130 364
MS.gene99541.t1 MTR_4g080740 97.238 181 5 0 14 194 1 181 1.73e-130 363
MS.gene99541.t1 MTR_3g007690 79.348 184 36 2 12 193 58 241 1.83e-101 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99541.t1 AT2G42740 95.580 181 8 0 14 194 1 181 5.52e-128 357
MS.gene99541.t1 AT5G45775 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene99541.t1 AT4G18730 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene99541.t1 AT3G58700 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene99541.t1 AT5G45775 95.906 171 7 0 24 194 1 171 2.69e-121 340

Find 52 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCTTCAACTGGTATTTA+TGG 0.121344 8.1:+59451442 MS.gene99541:CDS
GCTTGCTATGTGACTGTTAG+AGG 0.280983 8.1:+59450791 MS.gene99541:CDS
TTTAGTGATACCGGTTGTTT+TGG 0.285925 8.1:+59450881 MS.gene99541:CDS
TAAAATTACCTTTGCAGCTC+TGG 0.312435 8.1:-59448600 None:intergenic
AGATACACCGTCCGTTCTTT+TGG 0.314871 8.1:+59450746 MS.gene99541:CDS
TGACAAGGCAATGCAGCTTT+TGG 0.318529 8.1:+59450814 MS.gene99541:CDS
ACGACGTCCCACACGGTAAC+CGG 0.325434 8.1:-59451494 None:intergenic
CTTTGGAAAACACTGGGGTT+TGG 0.360701 8.1:-59448722 None:intergenic
CGATGCCATGAAGTGGTTCC+AGG 0.381480 8.1:+59451564 MS.gene99541:CDS
TTTCACCTGGAACCACTTCA+TGG 0.397811 8.1:-59451569 None:intergenic
CTCAGGTATGATCCTTCAAC+TGG 0.410746 8.1:+59451433 MS.gene99541:intron
CTTCCGTTTCCAACCTCAAA+TGG 0.415309 8.1:+59448399 MS.gene99541:CDS
TATTCAGGAGCATATTGATC+TGG 0.422822 8.1:+59450910 MS.gene99541:CDS
AAAAGAAAGCGTACCATTTG+AGG 0.439221 8.1:-59448412 None:intergenic
TTCGTTGTTCTGGAGCGTCC+CGG 0.445136 8.1:+59451475 MS.gene99541:CDS
CGTCGGTGCAAGGCTCGTGT+TGG 0.455767 8.1:+59451517 MS.gene99541:CDS
CTCGTTCTTAATATCTCTGT+TGG 0.461901 8.1:+59448557 MS.gene99541:CDS
CGTCCTTTGTTACGCGATGT+TGG 0.468785 8.1:-59451543 None:intergenic
GTCGGTGCAAGGCTCGTGTT+GGG 0.471084 8.1:+59451518 MS.gene99541:CDS
TTCAACTGGTATTTATGGAA+TGG 0.482662 8.1:+59451447 MS.gene99541:CDS
ATTCCATAAATACCAGTTGA+AGG 0.490800 8.1:-59451445 None:intergenic
GGAGAGTGGTTTGAAGGTGA+AGG 0.491764 8.1:+59450835 MS.gene99541:CDS
CTGAACCTTGATATCCCTCA+TGG 0.500440 8.1:-59448531 None:intergenic
TATAAACCTTTGGAAAACAC+TGG 0.500470 8.1:-59448729 None:intergenic
AATATCTCTGTTGGTGAAAG+TGG 0.507687 8.1:+59448566 MS.gene99541:CDS
GTACCATTTGAGGTTGGAAA+CGG 0.514068 8.1:-59448402 None:intergenic
GGGACGTCGTCGTCGGTGCA+AGG 0.515936 8.1:+59451507 MS.gene99541:CDS
CGACGTCCCACACGGTAACC+GGG 0.517440 8.1:-59451493 None:intergenic
ATAAACCTTTGGAAAACACT+GGG 0.526168 8.1:-59448728 None:intergenic
CAATCGCCTGTGACTTGTTG+AGG 0.534551 8.1:-59451606 None:intergenic
AATACCGAATCCAAAACAAC+CGG 0.535665 8.1:-59450891 None:intergenic
GAAAGCGTACCATTTGAGGT+TGG 0.546416 8.1:-59448408 None:intergenic
GAACACCATGAGGGATATCA+AGG 0.552248 8.1:+59448526 MS.gene99541:CDS
ATTCAGGAGCATATTGATCT+GGG 0.553824 8.1:+59450911 MS.gene99541:CDS
TGGTTCCAGGTGAAATATGA+AGG 0.565205 8.1:+59451577 MS.gene99541:CDS
TTCTTCTAATACCAAAAGAA+CGG 0.565907 8.1:-59450757 None:intergenic
ACCGTGTGGGACGTCGTCGT+CGG 0.567737 8.1:+59451500 MS.gene99541:CDS
GAGCGTCCCGGTTACCGTGT+GGG 0.573313 8.1:+59451487 MS.gene99541:CDS
TAACACCTTCATATTTCACC+TGG 0.578904 8.1:-59451582 None:intergenic
TCGTCTCACCAGAGCTGCAA+AGG 0.586053 8.1:+59448592 MS.gene99541:CDS
TGTTATCCTCAACAAGTCAC+AGG 0.598472 8.1:+59451600 MS.gene99541:CDS
GATCCAACATCGCGTAACAA+AGG 0.602047 8.1:+59451540 MS.gene99541:CDS
GGAGCGTCCCGGTTACCGTG+TGG 0.619085 8.1:+59451486 MS.gene99541:CDS
TGAGGTTGGAAACGGAAGAG+AGG 0.621017 8.1:-59448394 None:intergenic
TCTAATACCAAAAGAACGGA+CGG 0.628670 8.1:-59450753 None:intergenic
CAAAGGACGATGCCATGAAG+TGG 0.630614 8.1:+59451557 MS.gene99541:CDS
AGAAACTCTCGAACACCATG+AGG 0.653183 8.1:+59448516 MS.gene99541:CDS
ACCGACGACGACGTCCCACA+CGG 0.654712 8.1:-59451501 None:intergenic
TGTGACTGTTAGAGGTGACA+AGG 0.658203 8.1:+59450799 MS.gene99541:CDS
TAAACCTTTGGAAAACACTG+GGG 0.679293 8.1:-59448727 None:intergenic
GAAACTCTCGAACACCATGA+GGG 0.686507 8.1:+59448517 MS.gene99541:CDS
CAGGTGTTGGAACAACTCAG+TGG 0.734121 8.1:+59448699 MS.gene99541:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCAAAATAATAAAAATAAAT+TGG - chr8.1:59450523-59450542 None:intergenic 10.0%
!! TAAAGTTGAAAATTAAATTA+AGG - chr8.1:59448777-59448796 None:intergenic 10.0%
!!! TTTTTTTCTTTTTTACTAAA+AGG + chr8.1:59449722-59449741 MS.gene99541:intron 10.0%
!! AACTATTAAAATCAAAATCA+AGG - chr8.1:59449216-59449235 None:intergenic 15.0%
!! AATTTAGAAAACAAATGTTA+AGG - chr8.1:59449984-59450003 None:intergenic 15.0%
!! CAAAATTAAATATAAACCTT+TGG - chr8.1:59448742-59448761 None:intergenic 15.0%
!! TATTCTTGTATATTTGAATA+AGG + chr8.1:59449289-59449308 MS.gene99541:intron 15.0%
!! TTAATGAGAATTAATCTTAA+GGG + chr8.1:59450296-59450315 MS.gene99541:intron 15.0%
!! TTTAATGAGAATTAATCTTA+AGG + chr8.1:59450295-59450314 MS.gene99541:intron 15.0%
!!! AAAAAGTTTTACATATTTCT+AGG + chr8.1:59451209-59451228 MS.gene99541:intron 15.0%
!!! ATTTTCGTTTTTTTTCTTTT+TGG + chr8.1:59450718-59450737 MS.gene99541:intron 15.0%
!!! ATTTTGATTTTAATAGTTGA+GGG + chr8.1:59449218-59449237 MS.gene99541:intron 15.0%
!!! TATGATTGTTTATAAGTTTT+AGG + chr8.1:59450253-59450272 MS.gene99541:intron 15.0%
!!! TTTCATTTTTTGTATATATC+AGG + chr8.1:59448680-59448699 MS.gene99541:intron 15.0%
!! AACATAAATCATAACAAACT+TGG - chr8.1:59451398-59451417 None:intergenic 20.0%
!! AACTATTAAAATCACCTAAA+AGG + chr8.1:59449005-59449024 MS.gene99541:intron 20.0%
!! AATCAATATCCTAAAGTTTA+GGG + chr8.1:59448914-59448933 MS.gene99541:intron 20.0%
!! ATTACTATTCTATTTGATAC+TGG + chr8.1:59450136-59450155 MS.gene99541:intron 20.0%
!! CTTATATTAAAATGCCTAAA+TGG + chr8.1:59450049-59450068 MS.gene99541:intron 20.0%
!! GAGGAAAAAAAAAAAAAACA+AGG - chr8.1:59450181-59450200 None:intergenic 20.0%
!! TAAATTGACTGTTAATTCTA+GGG + chr8.1:59449164-59449183 MS.gene99541:intron 20.0%
!! TGAATGGCAATAAAAATTAA+GGG + chr8.1:59449356-59449375 MS.gene99541:intron 20.0%
!! TGATTTGAATGAGAATAAAA+TGG + chr8.1:59449815-59449834 MS.gene99541:intron 20.0%
!! TTACTATTCTATTTGATACT+GGG + chr8.1:59450137-59450156 MS.gene99541:intron 20.0%
!! TTGAATGGCAATAAAAATTA+AGG + chr8.1:59449355-59449374 MS.gene99541:intron 20.0%
!! TTTCATATCTATTAAATGCT+GGG + chr8.1:59450684-59450703 MS.gene99541:intron 20.0%
!!! AAAATTTGAAATTCAGTCTT+TGG + chr8.1:59451312-59451331 MS.gene99541:intron 20.0%
!!! GATTTTGATTTTAATAGTTG+AGG + chr8.1:59449217-59449236 MS.gene99541:intron 20.0%
!!! TAGATGATTTCAATAGTTTT+GGG + chr8.1:59449110-59449129 MS.gene99541:intron 20.0%
!!! TATTATTTTGCCTTGAAAAT+TGG + chr8.1:59450532-59450551 MS.gene99541:intron 20.0%
!!! TTCTTCTGGTATTTTAATTT+AGG - chr8.1:59449911-59449930 None:intergenic 20.0%
!!! TTTTCATATCTATTAAATGC+TGG + chr8.1:59450683-59450702 MS.gene99541:intron 20.0%
!!! TTTTGATTTTAATAGTTGAG+GGG + chr8.1:59449219-59449238 MS.gene99541:intron 20.0%
! AAAAACGAAAATCAGTTAAC+TGG - chr8.1:59450710-59450729 None:intergenic 25.0%
! AAAACAACCAAAAACTTTCA+AGG - chr8.1:59449448-59449467 None:intergenic 25.0%
! ACACTGTACATGAATATTAT+AGG + chr8.1:59450222-59450241 MS.gene99541:intron 25.0%
! ATAAATCATAACAAACTTGG+TGG - chr8.1:59451395-59451414 None:intergenic 25.0%
! ATCAATGTAGTGAAAAACAT+TGG - chr8.1:59449608-59449627 None:intergenic 25.0%
! CTAAATTGACTGTTAATTCT+AGG + chr8.1:59449163-59449182 MS.gene99541:intron 25.0%
! GAATCAATATCCTAAAGTTT+AGG + chr8.1:59448913-59448932 MS.gene99541:intron 25.0%
! GAATGAGAATAAAATGGAAA+GGG + chr8.1:59449821-59449840 MS.gene99541:intron 25.0%
! GTGTAATTCATGTTTAATAG+TGG + chr8.1:59449539-59449558 MS.gene99541:intron 25.0%
! TACTATTCTATTTGATACTG+GGG + chr8.1:59450138-59450157 MS.gene99541:intron 25.0%
! TGAATGAGAATAAAATGGAA+AGG + chr8.1:59449820-59449839 MS.gene99541:intron 25.0%
! TTCTTCTAATACCAAAAGAA+CGG - chr8.1:59450760-59450779 None:intergenic 25.0%
!! AAAACAACCAAAAGTTTTCA+TGG - chr8.1:59450409-59450428 None:intergenic 25.0%
!! CTAGATGATTTCAATAGTTT+TGG + chr8.1:59449109-59449128 MS.gene99541:intron 25.0%
!! TTCTTCTGGTATTTTCATTT+AGG - chr8.1:59449422-59449441 None:intergenic 25.0%
!! TTGTTTAAAACAGTTTCACA+TGG - chr8.1:59451045-59451064 None:intergenic 25.0%
!! TTTAACATTGTTTCTTTCTC+AGG + chr8.1:59451416-59451435 MS.gene99541:intron 25.0%
!! TTTTTGTATATATCAGGTGT+TGG + chr8.1:59448686-59448705 MS.gene99541:intron 25.0%
!!! AGAAGAAATTTTAGTGATAC+CGG + chr8.1:59450872-59450891 MS.gene99541:CDS 25.0%
!!! CTGTTGACTCAATTATAAAA+TGG - chr8.1:59449849-59449868 None:intergenic 25.0%
!!! GATAGTATTTTTGTGCTTAA+TGG + chr8.1:59448875-59448894 MS.gene99541:intron 25.0%
!!! GATCTAGTTTAAGTTTTGAA+TGG + chr8.1:59449340-59449359 MS.gene99541:intron 25.0%
!!! TTTTCAAGTCACAATAATCT+GGG + chr8.1:59450425-59450444 MS.gene99541:intron 25.0%
AAAACTTGATTTAGCATGCA+GGG - chr8.1:59450107-59450126 None:intergenic 30.0%
AAATTATGGCTAAAGCTATC+AGG + chr8.1:59451128-59451147 MS.gene99541:intron 30.0%
ACTATTCTATTTGATACTGG+GGG + chr8.1:59450139-59450158 MS.gene99541:intron 30.0%
AGATATGAAAACTTGACGAT+CGG - chr8.1:59450674-59450693 None:intergenic 30.0%
AGTTCTTCAAGAGGAAATTA+TGG + chr8.1:59451114-59451133 MS.gene99541:intron 30.0%
ATTATATTTGAGTCACAGCA+GGG + chr8.1:59450323-59450342 MS.gene99541:intron 30.0%
ATTCCATAAATACCAGTTGA+AGG - chr8.1:59451448-59451467 None:intergenic 30.0%
GATGGAATATCATCAGAAAT+CGG - chr8.1:59451371-59451390 None:intergenic 30.0%
GCTTAAAGACTGAAATGATA+AGG - chr8.1:59451341-59451360 None:intergenic 30.0%
GCTTAAAGAGACGTAAAAAT+CGG - chr8.1:59451284-59451303 None:intergenic 30.0%
GGAATGAAAATCAGAAAGAA+TGG - chr8.1:59448438-59448457 None:intergenic 30.0%
TAATGATTCAGTTCTTCAAG+AGG + chr8.1:59451105-59451124 MS.gene99541:intron 30.0%
TACAATCAACCCTAAACTTT+AGG - chr8.1:59448926-59448945 None:intergenic 30.0%
TATTATATTTGAGTCACAGC+AGG + chr8.1:59450322-59450341 MS.gene99541:intron 30.0%
TTATAATTGAGTCAACAGCA+GGG + chr8.1:59449851-59449870 MS.gene99541:intron 30.0%
TTCAACTGGTATTTATGGAA+TGG + chr8.1:59451447-59451466 MS.gene99541:CDS 30.0%
TTGAAAATTGGTTGACGATA+TGG + chr8.1:59450544-59450563 MS.gene99541:intron 30.0%
TTGTTTATACCAGTTTCACA+TGG - chr8.1:59451006-59451025 None:intergenic 30.0%
TTGTTTATCACAATGCATCT+TGG + chr8.1:59451236-59451255 MS.gene99541:intron 30.0%
TTTATAATTGAGTCAACAGC+AGG + chr8.1:59449850-59449869 MS.gene99541:intron 30.0%
! AGAAGATCCATGAAAACTTT+TGG + chr8.1:59450399-59450418 MS.gene99541:intron 30.0%
! AGAAGATCCTTGAAAGTTTT+TGG + chr8.1:59449438-59449457 MS.gene99541:intron 30.0%
! ATAAACCTTTGGAAAACACT+GGG - chr8.1:59448731-59448750 None:intergenic 30.0%
! ATATCGTCAACCAATTTTCA+AGG - chr8.1:59450545-59450564 None:intergenic 30.0%
! ATGCATTTTCTTACAGGTTT+CGG + chr8.1:59448490-59448509 MS.gene99541:intron 30.0%
! CGAAAAATGCATTTTCTTAC+AGG + chr8.1:59448484-59448503 MS.gene99541:intron 30.0%
! GTACATTTTATGGACCTTTT+AGG - chr8.1:59449022-59449041 None:intergenic 30.0%
! TATAAACCTTTGGAAAACAC+TGG - chr8.1:59448732-59448751 None:intergenic 30.0%
! TTCTTCTGGTATTTTCGTTT+AGG - chr8.1:59450383-59450402 None:intergenic 30.0%
! TTGTGCTTAATGGAGTTTTA+AGG + chr8.1:59448885-59448904 MS.gene99541:intron 30.0%
! TTTCAAGTCACAATAATCTG+GGG + chr8.1:59450426-59450445 MS.gene99541:intron 30.0%
!! AGGATGAGAAGTACATTTTA+TGG - chr8.1:59449032-59449051 None:intergenic 30.0%
!! GTTTTCAAGACACAATTATC+TGG + chr8.1:59449463-59449482 MS.gene99541:intron 30.0%
!!! AGTATTTTTTTGCTGCACAA+AGG + chr8.1:59449654-59449673 MS.gene99541:intron 30.0%
!!! ATTTTAATTTAGGCTCCAAG+CGG - chr8.1:59449901-59449920 None:intergenic 30.0%
!!! GTTTTCAAGTCACAATAATC+TGG + chr8.1:59450424-59450443 MS.gene99541:intron 30.0%
!!! TGATAGTTTTGTGTATGGAT+AGG + chr8.1:59449747-59449766 MS.gene99541:intron 30.0%
AACTTTCAAGGATCTTCTTC+TGG - chr8.1:59449436-59449455 None:intergenic 35.0%
AATACCGAATCCAAAACAAC+CGG - chr8.1:59450894-59450913 None:intergenic 35.0%
ACAGTTTCAACTCACCATTT+AGG - chr8.1:59450066-59450085 None:intergenic 35.0%
ATGGGTGGATGTACATTTAA+GGG + chr8.1:59449082-59449101 MS.gene99541:intron 35.0%
CTCGTTCTTAATATCTCTGT+TGG + chr8.1:59448557-59448576 MS.gene99541:CDS 35.0%
GAAAACTTGATTTAGCATGC+AGG - chr8.1:59450108-59450127 None:intergenic 35.0%
GAAGACAACAACAACAACAA+AGG - chr8.1:59448459-59448478 None:intergenic 35.0%
GATCCTTCAACTGGTATTTA+TGG + chr8.1:59451442-59451461 MS.gene99541:CDS 35.0%
GTACTTCTCATCCTTCAATT+TGG + chr8.1:59449038-59449057 MS.gene99541:intron 35.0%
TAAAATTACCTTTGCAGCTC+TGG - chr8.1:59448603-59448622 None:intergenic 35.0%
TAACACCTTCATATTTCACC+TGG - chr8.1:59451585-59451604 None:intergenic 35.0%
TAATAGTTGAGGGGTCAAAT+TGG + chr8.1:59449228-59449247 MS.gene99541:intron 35.0%
TATAATTGAGTCAACAGCAG+GGG + chr8.1:59449852-59449871 MS.gene99541:intron 35.0%
TATTATGTGCCATGTGAAAC+TGG + chr8.1:59450994-59451013 MS.gene99541:intron 35.0%
TCTAATACCAAAAGAACGGA+CGG - chr8.1:59450756-59450775 None:intergenic 35.0%
TGATAGAGAGCGATAGTTTA+GGG + chr8.1:59449251-59449270 MS.gene99541:intron 35.0%
TTATATTTGAGTCACAGCAG+GGG + chr8.1:59450324-59450343 MS.gene99541:intron 35.0%
! AAAAGAAAGCGTACCATTTG+AGG - chr8.1:59448415-59448434 None:intergenic 35.0%
! AATATCTCTGTTGGTGAAAG+TGG + chr8.1:59448566-59448585 MS.gene99541:CDS 35.0%
! AGTTTTCATGGATCTTCTTC+TGG - chr8.1:59450397-59450416 None:intergenic 35.0%
! ATTCAGGAGCATATTGATCT+GGG + chr8.1:59450911-59450930 MS.gene99541:CDS 35.0%
! ATTTTCATTTAGGCTCCAAG+CGG - chr8.1:59449412-59449431 None:intergenic 35.0%
! CTTGCTGTCAATAAGAAAAG+AGG - chr8.1:59450200-59450219 None:intergenic 35.0%
! TAAACCTTTGGAAAACACTG+GGG - chr8.1:59448730-59448749 None:intergenic 35.0%
! TATTCAGGAGCATATTGATC+TGG + chr8.1:59450910-59450929 MS.gene99541:CDS 35.0%
! TCCTTCAATTTGGTTTCCTA+CGG + chr8.1:59449048-59449067 MS.gene99541:intron 35.0%
!! TTTAGTGATACCGGTTGTTT+TGG + chr8.1:59450881-59450900 MS.gene99541:CDS 35.0%
!!! AATGGACTTTTTCGTTGTTC+TGG + chr8.1:59451465-59451484 MS.gene99541:CDS 35.0%
!!! AGGAGTGATAGTTTTGTGTA+TGG + chr8.1:59449742-59449761 MS.gene99541:intron 35.0%
!!! CAATAGTTTTGGGACTAAAC+TGG + chr8.1:59449120-59449139 MS.gene99541:intron 35.0%
!!! TTGTTTTGGATTCGGTATTC+AGG + chr8.1:59450895-59450914 MS.gene99541:CDS 35.0%
ACTGGTGAGAGTGATAGTTT+AGG + chr8.1:59449138-59449157 MS.gene99541:intron 40.0%
CCTACGGAGACTAAATTGAT+GGG + chr8.1:59449064-59449083 MS.gene99541:intron 40.0%
CTGGTGAGAGTGATAGTTTA+GGG + chr8.1:59449139-59449158 MS.gene99541:intron 40.0%
GATAGAGAGCGATAGTTTAG+GGG + chr8.1:59449252-59449271 MS.gene99541:intron 40.0%
GATGGGTGGATGTACATTTA+AGG + chr8.1:59449081-59449100 MS.gene99541:intron 40.0%
GTGATAGAGAGCGATAGTTT+AGG + chr8.1:59449250-59449269 MS.gene99541:intron 40.0%
TCCGTAGGAAACCAAATTGA+AGG - chr8.1:59449052-59449071 None:intergenic 40.0%
TCCTACGGAGACTAAATTGA+TGG + chr8.1:59449063-59449082 MS.gene99541:intron 40.0%
TGGTTCCAGGTGAAATATGA+AGG + chr8.1:59451577-59451596 MS.gene99541:CDS 40.0%
TGTTATCCTCAACAAGTCAC+AGG + chr8.1:59451600-59451619 MS.gene99541:CDS 40.0%
! ATTTTCGTTTAGGCTCCAAG+CGG - chr8.1:59450373-59450392 None:intergenic 40.0%
! GTACCATTTGAGGTTGGAAA+CGG - chr8.1:59448405-59448424 None:intergenic 40.0%
!! CATAACAAACTTGGTGGTGA+TGG - chr8.1:59451389-59451408 None:intergenic 40.0%
!!! GATACCGGTTGTTTTGGATT+CGG + chr8.1:59450887-59450906 MS.gene99541:CDS 40.0%
ACGGAGACTAAATTGATGGG+TGG + chr8.1:59449067-59449086 MS.gene99541:intron 45.0%
AGAAACTCTCGAACACCATG+AGG + chr8.1:59448516-59448535 MS.gene99541:CDS 45.0%
ATCCCTAATTGAGCTGCACA+TGG + chr8.1:59451183-59451202 MS.gene99541:intron 45.0%
CCCATCAATTTAGTCTCCGT+AGG - chr8.1:59449067-59449086 None:intergenic 45.0%
CTCAGGTATGATCCTTCAAC+TGG + chr8.1:59451433-59451452 MS.gene99541:intron 45.0%
CTGAACCTTGATATCCCTCA+TGG - chr8.1:59448534-59448553 None:intergenic 45.0%
CTTCCGTTTCCAACCTCAAA+TGG + chr8.1:59448399-59448418 MS.gene99541:CDS 45.0%
GAAACTCTCGAACACCATGA+GGG + chr8.1:59448517-59448536 MS.gene99541:CDS 45.0%
GAACACCATGAGGGATATCA+AGG + chr8.1:59448526-59448545 MS.gene99541:CDS 45.0%
GATCCAACATCGCGTAACAA+AGG + chr8.1:59451540-59451559 MS.gene99541:CDS 45.0%
TCCCATGTGCAGCTCAATTA+GGG - chr8.1:59451188-59451207 None:intergenic 45.0%
TCCCTAATTGAGCTGCACAT+GGG + chr8.1:59451184-59451203 MS.gene99541:intron 45.0%
TGTGACTGTTAGAGGTGACA+AGG + chr8.1:59450799-59450818 MS.gene99541:CDS 45.0%
TTTCACCTGGAACCACTTCA+TGG - chr8.1:59451572-59451591 None:intergenic 45.0%
! AGATACACCGTCCGTTCTTT+TGG + chr8.1:59450746-59450765 MS.gene99541:CDS 45.0%
! CAAACCCCAGTGTTTTCCAA+AGG + chr8.1:59448723-59448742 MS.gene99541:CDS 45.0%
! CTTTGGAAAACACTGGGGTT+TGG - chr8.1:59448725-59448744 None:intergenic 45.0%
! GAAAGCGTACCATTTGAGGT+TGG - chr8.1:59448411-59448430 None:intergenic 45.0%
! TGACAAGGCAATGCAGCTTT+TGG + chr8.1:59450814-59450833 MS.gene99541:CDS 45.0%
!! GCTTGCTATGTGACTGTTAG+AGG + chr8.1:59450791-59450810 MS.gene99541:CDS 45.0%
!! GCTTTTGGAGAGTGGTTTGA+AGG + chr8.1:59450829-59450848 MS.gene99541:CDS 45.0%
ATGACTACTTGACTGCCGCT+TGG + chr8.1:59449883-59449902 MS.gene99541:intron 50.0%
CAAAGGACGATGCCATGAAG+TGG + chr8.1:59451557-59451576 MS.gene99541:CDS 50.0%
CGTCCTTTGTTACGCGATGT+TGG - chr8.1:59451546-59451565 None:intergenic 50.0%
CTCCCATGTGCAGCTCAATT+AGG - chr8.1:59451189-59451208 None:intergenic 50.0%
GGAGAGTGGTTTGAAGGTGA+AGG + chr8.1:59450835-59450854 MS.gene99541:CDS 50.0%
TGAGGTTGGAAACGGAAGAG+AGG - chr8.1:59448397-59448416 None:intergenic 50.0%
!! CAATCGCCTGTGACTTGTTG+AGG - chr8.1:59451609-59451628 None:intergenic 50.0%
!! CAGGTGTTGGAACAACTCAG+TGG + chr8.1:59448699-59448718 MS.gene99541:intron 50.0%
!! GCAATGCAGCTTTTGGAGAG+TGG + chr8.1:59450821-59450840 MS.gene99541:CDS 50.0%
CGATGCCATGAAGTGGTTCC+AGG + chr8.1:59451564-59451583 MS.gene99541:CDS 55.0%
TCGTCTCACCAGAGCTGCAA+AGG + chr8.1:59448592-59448611 MS.gene99541:CDS 55.0%
!! TTCGTTGTTCTGGAGCGTCC+CGG + chr8.1:59451475-59451494 MS.gene99541:CDS 55.0%
ACGACGTCCCACACGGTAAC+CGG - chr8.1:59451497-59451516 None:intergenic 60.0%
ATGACTGCCTGACTGCCGCT+TGG + chr8.1:59450355-59450374 MS.gene99541:intron 60.0%
GAGTCAACAGCACTGCCGCT+TGG + chr8.1:59449394-59449413 MS.gene99541:intron 60.0%
GTCGGTGCAAGGCTCGTGTT+GGG + chr8.1:59451518-59451537 MS.gene99541:CDS 60.0%
TTAGGCTCCAAGCGGCAGTC+AGG - chr8.1:59449893-59449912 None:intergenic 60.0%
ACCGACGACGACGTCCCACA+CGG - chr8.1:59451504-59451523 None:intergenic 65.0%
ACCGTGTGGGACGTCGTCGT+CGG + chr8.1:59451500-59451519 MS.gene99541:CDS 65.0%
CGACGTCCCACACGGTAACC+GGG - chr8.1:59451496-59451515 None:intergenic 65.0%
CGTCGGTGCAAGGCTCGTGT+TGG + chr8.1:59451517-59451536 MS.gene99541:CDS 65.0%
GAGCGTCCCGGTTACCGTGT+GGG + chr8.1:59451487-59451506 MS.gene99541:CDS 65.0%
GGGACGTCGTCGTCGGTGCA+AGG + chr8.1:59451507-59451526 MS.gene99541:CDS 70.0%
! GGAGCGTCCCGGTTACCGTG+TGG + chr8.1:59451486-59451505 MS.gene99541:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 59448379 59451633 59448379 ID=MS.gene99541
chr8.1 mRNA 59448379 59451633 59448379 ID=MS.gene99541.t1;Parent=MS.gene99541
chr8.1 exon 59448379 59448420 59448379 ID=MS.gene99541.t1.exon1;Parent=MS.gene99541.t1
chr8.1 CDS 59448379 59448420 59448379 ID=cds.MS.gene99541.t1;Parent=MS.gene99541.t1
chr8.1 exon 59448506 59448613 59448506 ID=MS.gene99541.t1.exon2;Parent=MS.gene99541.t1
chr8.1 CDS 59448506 59448613 59448506 ID=cds.MS.gene99541.t1;Parent=MS.gene99541.t1
chr8.1 exon 59448702 59448744 59448702 ID=MS.gene99541.t1.exon3;Parent=MS.gene99541.t1
chr8.1 CDS 59448702 59448744 59448702 ID=cds.MS.gene99541.t1;Parent=MS.gene99541.t1
chr8.1 exon 59450744 59450939 59450744 ID=MS.gene99541.t1.exon4;Parent=MS.gene99541.t1
chr8.1 CDS 59450744 59450939 59450744 ID=cds.MS.gene99541.t1;Parent=MS.gene99541.t1
chr8.1 exon 59451438 59451633 59451438 ID=MS.gene99541.t1.exon5;Parent=MS.gene99541.t1
chr8.1 CDS 59451438 59451633 59451438 ID=cds.MS.gene99541.t1;Parent=MS.gene99541.t1
Gene Sequence

>MS.gene99541

ATGGCACAACAACAACCTCTCTTCCGTTTCCAACCTCAAATGGTACGCTTTCTTTTCCATTCTTTCTGATTTTCATTCCTTTGTTGTTGTTGTTGTCTTCTGAATCGAAAAATGCATTTTCTTACAGGTTTCGGAGAAGAAACTCTCGAACACCATGAGGGATATCAAGGTTCAGAAACTCGTTCTTAATATCTCTGTTGGTGAAAGTGGTGATCGTCTCACCAGAGCTGCAAAGGTAATTTTAATTTTAGTTTTAATTTTTACTTTTTTTTTTAATTTTATATATTTTTTTATTCTTACATTTCATTTTTTGTATATATCAGGTGTTGGAACAACTCAGTGGCCAAACCCCAGTGTTTTCCAAAGGTTTATATTTAATTTTGATTTTTTTGCTACCTTAATTTAATTTTCAACTTTAAATTAAATAAATAAGACTTTATTTTTATTTAAATATTAATATGTATGTATATGTATGTTGATTGATGTTAATGTAATTGATAGTATTTTTGTGCTTAATGGAGTTTTAAGGAATGTGAATCAATATCCTAAAGTTTAGGGTTGATTGTATATTGAATAAACAGTCAATTTAGTTTTTAAACTATTCACTCTCTTCAATTTGATTCGTAAACTATTAAAATCACCTAAAAGGTCCATAAAATGTACTTCTCATCCTTCAATTTGGTTTCCTACGGAGACTAAATTGATGGGTGGATGTACATTTAAGGGACTTCTAGATGATTTCAATAGTTTTGGGACTAAACTGGTGAGAGTGATAGTTTAGGGACTAAATTGACTGTTAATTCTAGGGACTAAATTGAACAATGATTGTGATATCCTTGATTTTGATTTTAATAGTTGAGGGGTCAAATTGGTGATAGAGAGCGATAGTTTAGGGGCTAAATTGACTGTTTATTCTTGTATATTTGAATAAGGTTGTTGATATGACTGTTCTTAGTGATTTGATCTAGTTTAAGTTTTGAATGGCAATAAAAATTAAGGGCTCTATTATTATAATGAGTCAACAGCACTGCCGCTTGGAGCCTAAATGAAAATACCAGAAGAAGATCCTTGAAAGTTTTTGGTTGTTTTCAAGACACAATTATCTGGTGAAATTCTTACGTTTGTATCGTATGTTAAATGTTAAATTGCATTGATAGATTGTGTAATTCATGTTTAATAGTGGCTGCTTTACTTGTTTTTGATTTTTTGTGTGTTTGTGTATTGCACCAATGTTTTTCACTACATTGATGTCGATGATTTATAAATGTTATTGATAGTATTTTTTTGCTGCACAAAGGAGTAACGTGAAATGTGAAATATCACAAAGTTTATTTGTGATTGTGTTTTTTTCTTTTTTACTAAAAGGAGTGATAGTTTTGTGTATGGATAGGATCATTGAAATGATAGTTCATAAGTTTATGAATTTGATCAAATTATGATTTGAATGAGAATAAAATGGAAAGGGCTCCATTTTATAATTGAGTCAACAGCAGGGGTCTGTTTAATGACTACTTGACTGCCGCTTGGAGCCTAAATTAAAATACCAGAAGAAGATCCTTGAAAGTTTTTGGTTGTTTTCAAGACACAATTATCTGGCGAAATCCTTAACATTTGTTTTCTAAATTGTAATACTAAAATTATAATTTTATATTTATTGTATATTGTCATAGCTTATATTAAAATGCCTAAATGGTGAGTTGAAACTGTTTTTGTTGAATTCATGTTCCCTGCATGCTAAATCAAGTTTTCTAGCTGCAATTACTATTCTATTTGATACTGGGGGTTGTTTGCTGATTGTTCCTTGTTTTTTTTTTTTTTCCTCTTTTCTTATTGACAGCAAGATACACTGTACATGAATATTATAGGATTATTGATATGATTGTTTATAAGTTTTAGGAACTCAAACAAATTATGTTTTTAATGAGAATTAATCTTAAGGGCTCTATTATATTTGAGTCACAGCAGGGGTCTGTTTAATGACTGCCTGACTGCCGCTTGGAGCCTAAACGAAAATACCAGAAGAAGATCCATGAAAACTTTTGGTTGTTTTCAAGTCACAATAATCTGGGGAAATTCTTACATTTGTATCATATCTTAAATGTTAAAGATATTTTAAATGCATATTTGATTTTTCATACACGCCAATTTATTTTTATTATTTTGCCTTGAAAATTGGTTGACGATATGGAATGATTATTTATTCTTGAATGATAAAATGTGTAAATTCGACTTGTTTTTAGCTGAAATAGTTTGTTTTGCTTTCATGCTAAGAAATCAAGTCTAGTGCAATCACCGATCGTCAAGTTTTCATATCTATTAAATGCTGGGCCAGTTAACTGATTTTCGTTTTTTTTCTTTTTGGCAGCTAGATACACCGTCCGTTCTTTTGGTATTAGAAGAAATGAAAAGATTGCTTGCTATGTGACTGTTAGAGGTGACAAGGCAATGCAGCTTTTGGAGAGTGGTTTGAAGGTGAAGGAATATGAATTGCTGAGAAGAAATTTTAGTGATACCGGTTGTTTTGGATTCGGTATTCAGGAGCATATTGATCTGGGAATCAAGTAAGCTCTAAATCTCTTGTATCTGTAACTAAAGTATATGTTGCACGTTATACTTATTATGTGCCATGTGAAACTGGTATAAACAAACTTTTCTAGAACGTGCCATGTGAAACTGTTTTAAACAAACTTTTCTAGAACTGCGTTATTTATGTTTCATTTATCTACTAATGATTCAGTTCTTCAAGAGGAAATTATGGCTAAAGCTATCAGGTGTTCAAAGTTTCGTTGTAGTGCTTGTAGCTCATCCCTAATTGAGCTGCACATGGGAGAAAAAGTTTTACATATTTCTAGGATAGTTGTTTATCACAATGCATCTTGGATTTGAAGTTAGCTGAGTTAATCCGATTTTTACGTCTCTTTAAGCTTATTATGAAAATTTGAAATTCAGTCTTTGGTTTCCTTATCATTTCAGTCTTTAAGCTTATAATCCGATTTCTGATGATATTCCATCACCACCAAGTTTGTTATGATTTATGTTTAACATTGTTTCTTTCTCAGGTATGATCCTTCAACTGGTATTTATGGAATGGACTTTTTCGTTGTTCTGGAGCGTCCCGGTTACCGTGTGGGACGTCGTCGTCGGTGCAAGGCTCGTGTTGGGATCCAACATCGCGTAACAAAGGACGATGCCATGAAGTGGTTCCAGGTGAAATATGAAGGTGTTATCCTCAACAAGTCACAGGCGATTGTTTAG
Protein sequence

>MS.gene99541.t1

MAQQQPLFRFQPQMVSEKKLSNTMRDIKVQKLVLNISVGESGDRLTRAAKVLEQLSGQTPVFSKARYTVRSFGIRRNEKIACYVTVRGDKAMQLLESGLKVKEYELLRRNFSDTGCFGFGIQEHIDLGIKYDPSTGIYGMDFFVVLERPGYRVGRRRRCKARVGIQHRVTKDDAMKWFQVKYEGVILNKSQAIV