AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066933

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066933.t1 MTR_8g046140 98.343 181 3 0 14 194 1 181 2.27e-131 366
MS.gene066933.t1 MTR_3g007700 98.333 180 3 0 15 194 3 182 1.36e-130 364
MS.gene066933.t1 MTR_4g080740 97.238 181 5 0 14 194 1 181 1.73e-130 363
MS.gene066933.t1 MTR_3g007690 79.348 184 36 2 12 193 58 241 1.83e-101 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066933.t1 AT2G42740 95.580 181 8 0 14 194 1 181 5.52e-128 357
MS.gene066933.t1 AT5G45775 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene066933.t1 AT4G18730 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene066933.t1 AT3G58700 95.028 181 9 0 14 194 1 181 2.56e-127 356
MS.gene066933.t1 AT5G45775 95.906 171 7 0 24 194 1 171 2.69e-121 340

Find 53 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCTTCAACTGGTATTTA+TGG 0.121344 8.4:+55227384 MS.gene066933:CDS
TTTCACCAACAGAGATATTA+AGG 0.271042 8.4:-55224454 None:intergenic
GCTTGCTATGTGACTGTTAG+AGG 0.280983 8.4:+55226733 MS.gene066933:CDS
TTTAGTGATACCGGTTGTTT+TGG 0.285925 8.4:+55226823 MS.gene066933:CDS
TAAAATTACCTTTGCAGCTC+TGG 0.312435 8.4:-55224492 None:intergenic
AGATACACCGTCCGTTCTTT+TGG 0.314871 8.4:+55226688 MS.gene066933:CDS
TGACAAGGCAATGCAGCTTT+TGG 0.318529 8.4:+55226756 MS.gene066933:CDS
ACGACGTCCCACACGGTAAC+CGG 0.325434 8.4:-55227436 None:intergenic
CTTTGGAAAACACTGGGGTT+TGG 0.360701 8.4:-55224663 None:intergenic
CGATGCCATGAAGTGGTTCC+AGG 0.381480 8.4:+55227506 MS.gene066933:CDS
TTTCACCTGGAACCACTTCA+TGG 0.397811 8.4:-55227511 None:intergenic
CTCAGGTATGATCCTTCAAC+TGG 0.410746 8.4:+55227375 MS.gene066933:intron
CTTCCGTTTCCAACCTCAAA+TGG 0.415309 8.4:+55224291 MS.gene066933:CDS
TATTCAGGAGCATATTGATC+TGG 0.422822 8.4:+55226852 MS.gene066933:CDS
AAAAGAAAGCGTACCATTTG+AGG 0.439221 8.4:-55224304 None:intergenic
TTCGTTGTTCTGGAGCGTCC+CGG 0.445136 8.4:+55227417 MS.gene066933:CDS
CGTCGGTGCAAGGCTCGTGT+TGG 0.455767 8.4:+55227459 MS.gene066933:CDS
CGTCCTTTGTTACGCGATGT+TGG 0.468785 8.4:-55227485 None:intergenic
GTCGGTGCAAGGCTCGTGTT+GGG 0.471084 8.4:+55227460 MS.gene066933:CDS
TTCAACTGGTATTTATGGAA+TGG 0.482662 8.4:+55227389 MS.gene066933:CDS
AATATCTCTGTTGGTGAAAG+TGG 0.486029 8.4:+55224458 MS.gene066933:CDS
ATTCCATAAATACCAGTTGA+AGG 0.490800 8.4:-55227387 None:intergenic
GGAGAGTGGTTTGAAGGTGA+AGG 0.491764 8.4:+55226777 MS.gene066933:CDS
CTTGTCCTTAATATCTCTGT+TGG 0.494069 8.4:+55224449 MS.gene066933:CDS
CTGAACCTTGATATCCCTCA+TGG 0.500440 8.4:-55224423 None:intergenic
AATAAACCTTTGGAAAACAC+TGG 0.500470 8.4:-55224670 None:intergenic
GTACCATTTGAGGTTGGAAA+CGG 0.514068 8.4:-55224294 None:intergenic
GGGACGTCGTCGTCGGTGCA+AGG 0.515936 8.4:+55227449 MS.gene066933:CDS
CGACGTCCCACACGGTAACC+GGG 0.517440 8.4:-55227435 None:intergenic
ATAAACCTTTGGAAAACACT+GGG 0.526168 8.4:-55224669 None:intergenic
CAATCGCCTGTGACTTGTTG+AGG 0.534551 8.4:-55227548 None:intergenic
AATACCGAATCCAAAACAAC+CGG 0.535665 8.4:-55226833 None:intergenic
GAAAGCGTACCATTTGAGGT+TGG 0.546416 8.4:-55224300 None:intergenic
GAACACCATGAGGGATATCA+AGG 0.552248 8.4:+55224418 MS.gene066933:CDS
ATTCAGGAGCATATTGATCT+GGG 0.553824 8.4:+55226853 MS.gene066933:CDS
TGGTTCCAGGTGAAATATGA+AGG 0.565205 8.4:+55227519 MS.gene066933:CDS
TTCTTCTAATACCAAAAGAA+CGG 0.565907 8.4:-55226699 None:intergenic
ACCGTGTGGGACGTCGTCGT+CGG 0.567737 8.4:+55227442 MS.gene066933:CDS
TCGTCTCACCAGAGCTGCAA+AGG 0.570040 8.4:+55224484 MS.gene066933:CDS
GAGCGTCCCGGTTACCGTGT+GGG 0.573313 8.4:+55227429 MS.gene066933:CDS
TAACACCTTCATATTTCACC+TGG 0.578904 8.4:-55227524 None:intergenic
TGTTATCCTCAACAAGTCAC+AGG 0.598472 8.4:+55227542 MS.gene066933:CDS
GATCCAACATCGCGTAACAA+AGG 0.602047 8.4:+55227482 MS.gene066933:CDS
GGAGCGTCCCGGTTACCGTG+TGG 0.619085 8.4:+55227428 MS.gene066933:CDS
TGAGGTTGGAAACGGAAGAG+AGG 0.621017 8.4:-55224286 None:intergenic
TCTAATACCAAAAGAACGGA+CGG 0.628670 8.4:-55226695 None:intergenic
CAAAGGACGATGCCATGAAG+TGG 0.630614 8.4:+55227499 MS.gene066933:CDS
AGAAACTCTCGAACACCATG+AGG 0.653183 8.4:+55224408 MS.gene066933:CDS
ACCGACGACGACGTCCCACA+CGG 0.654712 8.4:-55227443 None:intergenic
TGTGACTGTTAGAGGTGACA+AGG 0.658203 8.4:+55226741 MS.gene066933:CDS
TAAACCTTTGGAAAACACTG+GGG 0.679293 8.4:-55224668 None:intergenic
GAAACTCTCGAACACCATGA+GGG 0.686507 8.4:+55224409 MS.gene066933:CDS
CAGGTGTTGGAACAACTCAG+TGG 0.734121 8.4:+55224640 MS.gene066933:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCAAAATAATAAAAATAAAT+TGG - chr8.4:55226465-55226484 None:intergenic 10.0%
!!! TTTTTTTCTTTTTTACTAAA+AGG + chr8.4:55225654-55225673 MS.gene066933:intron 10.0%
!! AACTATTAAAATCAAAATCA+AGG - chr8.4:55225124-55225143 None:intergenic 15.0%
!! CAAAATTAAAAATAAACCTT+TGG - chr8.4:55224683-55224702 None:intergenic 15.0%
!! GGAAAAAAAAAAAAAAAACA+AGG - chr8.4:55226121-55226140 None:intergenic 15.0%
!! TATTCTTGTATATTTGAATA+AGG + chr8.4:55225197-55225216 MS.gene066933:intron 15.0%
!! TTAATGAGAATTAATCTTAA+GGG + chr8.4:55226238-55226257 MS.gene066933:intron 15.0%
!! TTTAATGAGAATTAATCTTA+AGG + chr8.4:55226237-55226256 MS.gene066933:intron 15.0%
!!! AAAAAGTTTTACATAATTCT+AGG + chr8.4:55227151-55227170 MS.gene066933:intron 15.0%
!!! ATTTTCGTTTTTTTTCTTTT+TGG + chr8.4:55226660-55226679 MS.gene066933:intron 15.0%
!!! ATTTTGATTTTAATAGTTGA+GGG + chr8.4:55225126-55225145 MS.gene066933:intron 15.0%
!!! TAGATGATTTTAATAGTTTA+GGG + chr8.4:55225018-55225037 MS.gene066933:intron 15.0%
!!! TATGATTGTTTATAAGTTTT+AGG + chr8.4:55226195-55226214 MS.gene066933:intron 15.0%
!! AACATAAATCATAACAAACT+TGG - chr8.4:55227340-55227359 None:intergenic 20.0%
!! AACTATTAAAATCACCTAAA+AGG + chr8.4:55224914-55224933 MS.gene066933:intron 20.0%
!! AATCAATATCCTAAAGTTTA+GGG + chr8.4:55224823-55224842 MS.gene066933:intron 20.0%
!! ATTACTATTCTATTTGATAC+TGG + chr8.4:55226076-55226095 MS.gene066933:intron 20.0%
!! CATTTAGAAAACAAATGTTA+AGG - chr8.4:55225916-55225935 None:intergenic 20.0%
!! CTTAAATTAAAATGCCTAAA+TGG + chr8.4:55225980-55225999 MS.gene066933:intron 20.0%
!! TAAATTGACTGTTAATTCTA+GGG + chr8.4:55225072-55225091 MS.gene066933:intron 20.0%
!! TGAATGGCAATAAAAATTAA+GGG + chr8.4:55225264-55225283 MS.gene066933:intron 20.0%
!! TGATTTGAATGAGAATAAAA+TGG + chr8.4:55225747-55225766 MS.gene066933:intron 20.0%
!! TTACTATTCTATTTGATACT+GGG + chr8.4:55226077-55226096 MS.gene066933:intron 20.0%
!! TTGAATGGCAATAAAAATTA+AGG + chr8.4:55225263-55225282 MS.gene066933:intron 20.0%
!! TTTCATATCTATTAAATGCT+GGG + chr8.4:55226626-55226645 MS.gene066933:intron 20.0%
!!! AAAATTTGAAATTCAGTCTT+TGG + chr8.4:55227254-55227273 MS.gene066933:intron 20.0%
!!! CTAGATGATTTTAATAGTTT+AGG + chr8.4:55225017-55225036 MS.gene066933:intron 20.0%
!!! GATTTTGATTTTAATAGTTG+AGG + chr8.4:55225125-55225144 MS.gene066933:intron 20.0%
!!! GTTCATTTTTTGTATTTATC+AGG + chr8.4:55224621-55224640 MS.gene066933:intron 20.0%
!!! TATTATTTTGCCTTGAAAAT+TGG + chr8.4:55226474-55226493 MS.gene066933:intron 20.0%
!!! TTCTTCTGGTATTTTAATTT+AGG - chr8.4:55225843-55225862 None:intergenic 20.0%
!!! TTTTCATATCTATTAAATGC+TGG + chr8.4:55226625-55226644 MS.gene066933:intron 20.0%
!!! TTTTGATTTTAATAGTTGAG+GGG + chr8.4:55225127-55225146 MS.gene066933:intron 20.0%
! AAAAACGAAAATCAGTTAAC+TGG - chr8.4:55226652-55226671 None:intergenic 25.0%
! AAAACAACCAAAAACTTTCA+AGG - chr8.4:55225380-55225399 None:intergenic 25.0%
! ACACTGTACATGAATATTAT+AGG + chr8.4:55226164-55226183 MS.gene066933:intron 25.0%
! ATAAATCATAACAAACTTGG+TGG - chr8.4:55227337-55227356 None:intergenic 25.0%
! ATCAATGTAGTGAAAAACAT+TGG - chr8.4:55225540-55225559 None:intergenic 25.0%
! CTAAATTGACTGTTAATTCT+AGG + chr8.4:55225071-55225090 MS.gene066933:intron 25.0%
! GAATCAATATCCTAAAGTTT+AGG + chr8.4:55224822-55224841 MS.gene066933:intron 25.0%
! GAATGAGAATAAAATGGAAA+GGG + chr8.4:55225753-55225772 MS.gene066933:intron 25.0%
! GTGTAATTCATGTTTAATAG+TGG + chr8.4:55225471-55225490 MS.gene066933:intron 25.0%
! TACTATTCTATTTGATACTG+GGG + chr8.4:55226078-55226097 MS.gene066933:intron 25.0%
! TGAATGAGAATAAAATGGAA+AGG + chr8.4:55225752-55225771 MS.gene066933:intron 25.0%
! TTCTTCTAATACCAAAAGAA+CGG - chr8.4:55226702-55226721 None:intergenic 25.0%
!! AAAACAACCAAAAGTTTTCA+TGG - chr8.4:55226351-55226370 None:intergenic 25.0%
!! TTCTTCTGGTATTTTCATTT+AGG - chr8.4:55225354-55225373 None:intergenic 25.0%
!! TTGTTTAAAACAGTTTCACA+TGG - chr8.4:55226987-55227006 None:intergenic 25.0%
!! TTTAACATTGTTTCTTTCTC+AGG + chr8.4:55227358-55227377 MS.gene066933:intron 25.0%
!! TTTTTGTATTTATCAGGTGT+TGG + chr8.4:55224627-55224646 MS.gene066933:intron 25.0%
!!! AGAAGAAATTTTAGTGATAC+CGG + chr8.4:55226814-55226833 MS.gene066933:CDS 25.0%
!!! CTGTTGACTCAATTATAAAA+TGG - chr8.4:55225781-55225800 None:intergenic 25.0%
!!! GATCTAGTTTAAGTTTTGAA+TGG + chr8.4:55225248-55225267 MS.gene066933:intron 25.0%
!!! TTTTCAAGTCACAATAATCT+GGG + chr8.4:55226367-55226386 MS.gene066933:intron 25.0%
AAAACTTGATTTAGCATGCA+GGG - chr8.4:55226047-55226066 None:intergenic 30.0%
AAATTATGGCTAAAGCTATC+AGG + chr8.4:55227070-55227089 MS.gene066933:intron 30.0%
AACAGTTTCAACTCAAAACA+TGG - chr8.4:55226007-55226026 None:intergenic 30.0%
ACAGTTTCAACTCAAAACAT+GGG - chr8.4:55226006-55226025 None:intergenic 30.0%
ACTATTCTATTTGATACTGG+GGG + chr8.4:55226079-55226098 MS.gene066933:intron 30.0%
AGATATGAAAACTTGACGAT+CGG - chr8.4:55226616-55226635 None:intergenic 30.0%
AGTTCTTCAAGAGGAAATTA+TGG + chr8.4:55227056-55227075 MS.gene066933:intron 30.0%
ATTATAATGAGTCAACAGCA+GGG + chr8.4:55225294-55225313 MS.gene066933:intron 30.0%
ATTATATTTGAGTCACAGCA+GGG + chr8.4:55226265-55226284 MS.gene066933:intron 30.0%
ATTCCATAAATACCAGTTGA+AGG - chr8.4:55227390-55227409 None:intergenic 30.0%
GAAAACAACAACAACAACAA+AGG - chr8.4:55224351-55224370 None:intergenic 30.0%
GATGGAATATCATCAGAAAT+CGG - chr8.4:55227313-55227332 None:intergenic 30.0%
GCTTAAAGACTGAAATGATA+AGG - chr8.4:55227283-55227302 None:intergenic 30.0%
GCTTAAAGAGACGTAAAAAT+CGG - chr8.4:55227226-55227245 None:intergenic 30.0%
GGAATGAAAATCAGAAAGAA+TGG - chr8.4:55224330-55224349 None:intergenic 30.0%
TAATAGTTTAGGGACTAAAC+TGG + chr8.4:55225028-55225047 MS.gene066933:intron 30.0%
TAATGATTCAGTTCTTCAAG+AGG + chr8.4:55227047-55227066 MS.gene066933:intron 30.0%
TACAATCAACCCTAAACTTT+AGG - chr8.4:55224835-55224854 None:intergenic 30.0%
TATTATAATGAGTCAACAGC+AGG + chr8.4:55225293-55225312 MS.gene066933:intron 30.0%
TATTATATTTGAGTCACAGC+AGG + chr8.4:55226264-55226283 MS.gene066933:intron 30.0%
TTATAATTGAGTCAACAGCA+GGG + chr8.4:55225783-55225802 MS.gene066933:intron 30.0%
TTCAACTGGTATTTATGGAA+TGG + chr8.4:55227389-55227408 MS.gene066933:CDS 30.0%
TTGAAAATTGGTTGACGATA+TGG + chr8.4:55226486-55226505 MS.gene066933:intron 30.0%
TTGTTTATACCAGTTTCACA+TGG - chr8.4:55226948-55226967 None:intergenic 30.0%
TTGTTTATCACAATGCATGT+TGG + chr8.4:55227178-55227197 MS.gene066933:intron 30.0%
TTTATAATTGAGTCAACAGC+AGG + chr8.4:55225782-55225801 MS.gene066933:intron 30.0%
TTTCACCAACAGAGATATTA+AGG - chr8.4:55224457-55224476 None:intergenic 30.0%
! AATAAACCTTTGGAAAACAC+TGG - chr8.4:55224673-55224692 None:intergenic 30.0%
! AGAAGATCCATGAAAACTTT+TGG + chr8.4:55226341-55226360 MS.gene066933:intron 30.0%
! AGAAGATCCTTGAAAGTTTT+TGG + chr8.4:55225370-55225389 MS.gene066933:intron 30.0%
! ATAAACCTTTGGAAAACACT+GGG - chr8.4:55224672-55224691 None:intergenic 30.0%
! ATATCGTCAACCAATTTTCA+AGG - chr8.4:55226487-55226506 None:intergenic 30.0%
! ATGCATTTTCTTACAGGTTT+CGG + chr8.4:55224382-55224401 MS.gene066933:intron 30.0%
! CGAAAAATGCATTTTCTTAC+AGG + chr8.4:55224376-55224395 MS.gene066933:intron 30.0%
! GTACATTTTATGGACCTTTT+AGG - chr8.4:55224931-55224950 None:intergenic 30.0%
! TTCTTCTGGTATTTTCGTTT+AGG - chr8.4:55226325-55226344 None:intergenic 30.0%
! TTGTGGTTAATGGAGTTTTA+AGG + chr8.4:55224794-55224813 MS.gene066933:intron 30.0%
! TTTCAAGTCACAATAATCTG+GGG + chr8.4:55226368-55226387 MS.gene066933:intron 30.0%
!! AGGATGAGAAGTACATTTTA+TGG - chr8.4:55224941-55224960 None:intergenic 30.0%
!! GATAGTGTTTTTGTGGTTAA+TGG + chr8.4:55224784-55224803 MS.gene066933:intron 30.0%
!! GTTTTCAAGACACAATTATC+TGG + chr8.4:55225395-55225414 MS.gene066933:intron 30.0%
!!! AGTATTTTTTTGCTGCACAA+AGG + chr8.4:55225586-55225605 MS.gene066933:intron 30.0%
!!! ATTTTAATTTAGGCTCCAAG+CGG - chr8.4:55225833-55225852 None:intergenic 30.0%
!!! CGTAATTGATAGTGTTTTTG+TGG + chr8.4:55224777-55224796 MS.gene066933:intron 30.0%
!!! GTTTTCAAGTCACAATAATC+TGG + chr8.4:55226366-55226385 MS.gene066933:intron 30.0%
!!! TGATAGTTTTGTGTATGGAT+AGG + chr8.4:55225679-55225698 MS.gene066933:intron 30.0%
AACTTTCAAGGATCTTCTTC+TGG - chr8.4:55225368-55225387 None:intergenic 35.0%
AATACCGAATCCAAAACAAC+CGG - chr8.4:55226836-55226855 None:intergenic 35.0%
CTTGTCCTTAATATCTCTGT+TGG + chr8.4:55224449-55224468 MS.gene066933:CDS 35.0%
GAAAACTTGATTTAGCATGC+AGG - chr8.4:55226048-55226067 None:intergenic 35.0%
GATCCTTCAACTGGTATTTA+TGG + chr8.4:55227384-55227403 MS.gene066933:CDS 35.0%
GTACTTCTCATCCTTCAATT+TGG + chr8.4:55224947-55224966 MS.gene066933:intron 35.0%
TAAAATTACCTTTGCAGCTC+TGG - chr8.4:55224495-55224514 None:intergenic 35.0%
TAACACCTTCATATTTCACC+TGG - chr8.4:55227527-55227546 None:intergenic 35.0%
TAATAGTTGAGGGGTCAAAT+TGG + chr8.4:55225136-55225155 MS.gene066933:intron 35.0%
TATAATTGAGTCAACAGCAG+GGG + chr8.4:55225784-55225803 MS.gene066933:intron 35.0%
TATTATGTGCCATGTGAAAC+TGG + chr8.4:55226936-55226955 MS.gene066933:intron 35.0%
TCTAATACCAAAAGAACGGA+CGG - chr8.4:55226698-55226717 None:intergenic 35.0%
TGATAGAGAGCGATAGTTTA+GGG + chr8.4:55225159-55225178 MS.gene066933:intron 35.0%
TTATAATGAGTCAACAGCAG+GGG + chr8.4:55225295-55225314 MS.gene066933:intron 35.0%
TTATATTTGAGTCACAGCAG+GGG + chr8.4:55226266-55226285 MS.gene066933:intron 35.0%
! AAAAGAAAGCGTACCATTTG+AGG - chr8.4:55224307-55224326 None:intergenic 35.0%
! AATATCTCTGTTGGTGAAAG+TGG + chr8.4:55224458-55224477 MS.gene066933:CDS 35.0%
! AGTTTTCATGGATCTTCTTC+TGG - chr8.4:55226339-55226358 None:intergenic 35.0%
! ATTCAGGAGCATATTGATCT+GGG + chr8.4:55226853-55226872 MS.gene066933:CDS 35.0%
! ATTTTCATTTAGGCTCCAAG+CGG - chr8.4:55225344-55225363 None:intergenic 35.0%
! CTTGCTGTCAATAAGAAAAG+AGG - chr8.4:55226142-55226161 None:intergenic 35.0%
! TAAACCTTTGGAAAACACTG+GGG - chr8.4:55224671-55224690 None:intergenic 35.0%
! TATTCAGGAGCATATTGATC+TGG + chr8.4:55226852-55226871 MS.gene066933:CDS 35.0%
! TCCTTCAATTTGGTTTCCTA+CGG + chr8.4:55224957-55224976 MS.gene066933:intron 35.0%
!! GATGGGTGGATTACATTTAA+GGG + chr8.4:55224990-55225009 MS.gene066933:intron 35.0%
!! TGATGGGTGGATTACATTTA+AGG + chr8.4:55224989-55225008 MS.gene066933:intron 35.0%
!! TTTAGTGATACCGGTTGTTT+TGG + chr8.4:55226823-55226842 MS.gene066933:CDS 35.0%
!!! AATGGACTTTTTCGTTGTTC+TGG + chr8.4:55227407-55227426 MS.gene066933:CDS 35.0%
!!! AGGAGTGATAGTTTTGTGTA+TGG + chr8.4:55225674-55225693 MS.gene066933:intron 35.0%
!!! TTGTTTTGGATTCGGTATTC+AGG + chr8.4:55226837-55226856 MS.gene066933:CDS 35.0%
ACTCAAAACATGGGCCATTT+AGG - chr8.4:55225997-55226016 None:intergenic 40.0%
ACTGGTGAGAGTGATAGTTT+AGG + chr8.4:55225046-55225065 MS.gene066933:intron 40.0%
CCTACGGAGACTAAATTGAT+GGG + chr8.4:55224973-55224992 MS.gene066933:intron 40.0%
CTGGTGAGAGTGATAGTTTA+GGG + chr8.4:55225047-55225066 MS.gene066933:intron 40.0%
GATAGAGAGCGATAGTTTAG+GGG + chr8.4:55225160-55225179 MS.gene066933:intron 40.0%
GTGATAGAGAGCGATAGTTT+AGG + chr8.4:55225158-55225177 MS.gene066933:intron 40.0%
TCCGTAGGAAACCAAATTGA+AGG - chr8.4:55224961-55224980 None:intergenic 40.0%
TCCTACGGAGACTAAATTGA+TGG + chr8.4:55224972-55224991 MS.gene066933:intron 40.0%
TGGTTCCAGGTGAAATATGA+AGG + chr8.4:55227519-55227538 MS.gene066933:CDS 40.0%
TGTTATCCTCAACAAGTCAC+AGG + chr8.4:55227542-55227561 MS.gene066933:CDS 40.0%
! ATTTTCGTTTAGGCTCCAAG+CGG - chr8.4:55226315-55226334 None:intergenic 40.0%
! GTACCATTTGAGGTTGGAAA+CGG - chr8.4:55224297-55224316 None:intergenic 40.0%
!! CATAACAAACTTGGTGGTGA+TGG - chr8.4:55227331-55227350 None:intergenic 40.0%
!!! GATACCGGTTGTTTTGGATT+CGG + chr8.4:55226829-55226848 MS.gene066933:CDS 40.0%
ACGGAGACTAAATTGATGGG+TGG + chr8.4:55224976-55224995 MS.gene066933:intron 45.0%
AGAAACTCTCGAACACCATG+AGG + chr8.4:55224408-55224427 MS.gene066933:CDS 45.0%
ATCCCTAATTGAGCTGCACA+TGG + chr8.4:55227125-55227144 MS.gene066933:intron 45.0%
CCCATCAATTTAGTCTCCGT+AGG - chr8.4:55224976-55224995 None:intergenic 45.0%
CTCAGGTATGATCCTTCAAC+TGG + chr8.4:55227375-55227394 MS.gene066933:intron 45.0%
CTGAACCTTGATATCCCTCA+TGG - chr8.4:55224426-55224445 None:intergenic 45.0%
CTTCCGTTTCCAACCTCAAA+TGG + chr8.4:55224291-55224310 MS.gene066933:CDS 45.0%
GAAACTCTCGAACACCATGA+GGG + chr8.4:55224409-55224428 MS.gene066933:CDS 45.0%
GAACACCATGAGGGATATCA+AGG + chr8.4:55224418-55224437 MS.gene066933:CDS 45.0%
GATCCAACATCGCGTAACAA+AGG + chr8.4:55227482-55227501 MS.gene066933:CDS 45.0%
TCCCATGTGCAGCTCAATTA+GGG - chr8.4:55227130-55227149 None:intergenic 45.0%
TCCCTAATTGAGCTGCACAT+GGG + chr8.4:55227126-55227145 MS.gene066933:intron 45.0%
TGTGACTGTTAGAGGTGACA+AGG + chr8.4:55226741-55226760 MS.gene066933:CDS 45.0%
TTTCACCTGGAACCACTTCA+TGG - chr8.4:55227514-55227533 None:intergenic 45.0%
! AGATACACCGTCCGTTCTTT+TGG + chr8.4:55226688-55226707 MS.gene066933:CDS 45.0%
! CAAACCCCAGTGTTTTCCAA+AGG + chr8.4:55224664-55224683 MS.gene066933:CDS 45.0%
! CTTTGGAAAACACTGGGGTT+TGG - chr8.4:55224666-55224685 None:intergenic 45.0%
! GAAAGCGTACCATTTGAGGT+TGG - chr8.4:55224303-55224322 None:intergenic 45.0%
! TGACAAGGCAATGCAGCTTT+TGG + chr8.4:55226756-55226775 MS.gene066933:CDS 45.0%
!! GCTTGCTATGTGACTGTTAG+AGG + chr8.4:55226733-55226752 MS.gene066933:CDS 45.0%
!! GCTTTTGGAGAGTGGTTTGA+AGG + chr8.4:55226771-55226790 MS.gene066933:CDS 45.0%
ATGACTACTTGACTGCCGCT+TGG + chr8.4:55225815-55225834 MS.gene066933:intron 50.0%
CAAAGGACGATGCCATGAAG+TGG + chr8.4:55227499-55227518 MS.gene066933:CDS 50.0%
CGTCCTTTGTTACGCGATGT+TGG - chr8.4:55227488-55227507 None:intergenic 50.0%
CTCCCATGTGCAGCTCAATT+AGG - chr8.4:55227131-55227150 None:intergenic 50.0%
GGAGAGTGGTTTGAAGGTGA+AGG + chr8.4:55226777-55226796 MS.gene066933:CDS 50.0%
TGAGGTTGGAAACGGAAGAG+AGG - chr8.4:55224289-55224308 None:intergenic 50.0%
!! CAATCGCCTGTGACTTGTTG+AGG - chr8.4:55227551-55227570 None:intergenic 50.0%
!! CAGGTGTTGGAACAACTCAG+TGG + chr8.4:55224640-55224659 MS.gene066933:intron 50.0%
!! GCAATGCAGCTTTTGGAGAG+TGG + chr8.4:55226763-55226782 MS.gene066933:CDS 50.0%
ATGACTACCTGACTGCCGCT+TGG + chr8.4:55225326-55225345 MS.gene066933:intron 55.0%
CGATGCCATGAAGTGGTTCC+AGG + chr8.4:55227506-55227525 MS.gene066933:CDS 55.0%
TCGTCTCACCAGAGCTGCAA+AGG + chr8.4:55224484-55224503 MS.gene066933:CDS 55.0%
!! TTCGTTGTTCTGGAGCGTCC+CGG + chr8.4:55227417-55227436 MS.gene066933:CDS 55.0%
ACGACGTCCCACACGGTAAC+CGG - chr8.4:55227439-55227458 None:intergenic 60.0%
ATGACTGCCTGACTGCCGCT+TGG + chr8.4:55226297-55226316 MS.gene066933:intron 60.0%
GTCGGTGCAAGGCTCGTGTT+GGG + chr8.4:55227460-55227479 MS.gene066933:CDS 60.0%
TTAGGCTCCAAGCGGCAGTC+AGG - chr8.4:55225336-55225355 None:intergenic 60.0%
ACCGACGACGACGTCCCACA+CGG - chr8.4:55227446-55227465 None:intergenic 65.0%
ACCGTGTGGGACGTCGTCGT+CGG + chr8.4:55227442-55227461 MS.gene066933:CDS 65.0%
CGACGTCCCACACGGTAACC+GGG - chr8.4:55227438-55227457 None:intergenic 65.0%
CGTCGGTGCAAGGCTCGTGT+TGG + chr8.4:55227459-55227478 MS.gene066933:CDS 65.0%
GAGCGTCCCGGTTACCGTGT+GGG + chr8.4:55227429-55227448 MS.gene066933:CDS 65.0%
GGGACGTCGTCGTCGGTGCA+AGG + chr8.4:55227449-55227468 MS.gene066933:CDS 70.0%
! GGAGCGTCCCGGTTACCGTG+TGG + chr8.4:55227428-55227447 MS.gene066933:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 55224271 55227575 55224271 ID=MS.gene066933
chr8.4 mRNA 55224271 55227575 55224271 ID=MS.gene066933.t1;Parent=MS.gene066933
chr8.4 exon 55224271 55224312 55224271 ID=MS.gene066933.t1.exon1;Parent=MS.gene066933.t1
chr8.4 CDS 55224271 55224312 55224271 ID=cds.MS.gene066933.t1;Parent=MS.gene066933.t1
chr8.4 exon 55224398 55224505 55224398 ID=MS.gene066933.t1.exon2;Parent=MS.gene066933.t1
chr8.4 CDS 55224398 55224505 55224398 ID=cds.MS.gene066933.t1;Parent=MS.gene066933.t1
chr8.4 exon 55224643 55224685 55224643 ID=MS.gene066933.t1.exon3;Parent=MS.gene066933.t1
chr8.4 CDS 55224643 55224685 55224643 ID=cds.MS.gene066933.t1;Parent=MS.gene066933.t1
chr8.4 exon 55226686 55226881 55226686 ID=MS.gene066933.t1.exon4;Parent=MS.gene066933.t1
chr8.4 CDS 55226686 55226881 55226686 ID=cds.MS.gene066933.t1;Parent=MS.gene066933.t1
chr8.4 exon 55227380 55227575 55227380 ID=MS.gene066933.t1.exon5;Parent=MS.gene066933.t1
chr8.4 CDS 55227380 55227575 55227380 ID=cds.MS.gene066933.t1;Parent=MS.gene066933.t1
Gene Sequence

>MS.gene066933

ATGGCACAACAACAACCTCTCTTCCGTTTCCAACCTCAAATGGTACGCTTTCTTTTCCATTCTTTCTGATTTTCATTCCTTTGTTGTTGTTGTTGTTTTCTGAATCGAAAAATGCATTTTCTTACAGGTTTCGGAGAAGAAACTCTCGAACACCATGAGGGATATCAAGGTTCAGAAACTTGTCCTTAATATCTCTGTTGGTGAAAGTGGCGATCGTCTCACCAGAGCTGCAAAGGTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTTACTTTTTTTTTTGAATTGTATATATTTTATATTCTAACATGTTCATTTTTTGTATTTATCAGGTGTTGGAACAACTCAGTGGCCAAACCCCAGTGTTTTCCAAAGGTTTATTTTTAATTTTGATTTTTCGCTATCTTAATTTGATTGTCAAATTTAAATATTAATATGTATGTATATGTATATTCATTGATGTTAACGTAATTGATAGTGTTTTTGTGGTTAATGGAGTTTTAAGGAATGTGAATCAATATCCTAAAGTTTAGGGTTGATTGTATATTGAATAAACAGTCAATTTAGTTTTTAAACTATTCACTCTCTTCAATTTGATTCGTAAACTATTAAAATCACCTAAAAGGTCCATAAAATGTACTTCTCATCCTTCAATTTGGTTTCCTACGGAGACTAAATTGATGGGTGGATTACATTTAAGGGACTTCTAGATGATTTTAATAGTTTAGGGACTAAACTGGTGAGAGTGATAGTTTAGGGACTAAATTGACTGTTAATTCTAGGGACTAAATTGAACAATGATTGTGATATCCTTGATTTTGATTTTAATAGTTGAGGGGTCAAATTGGTGATAGAGAGCGATAGTTTAGGGGCTAAATTGACTGTTTATTCTTGTATATTTGAATAAGGTTGTTGATATGACTGTTCTTAGTGATTTGATCTAGTTTAAGTTTTGAATGGCAATAAAAATTAAGGGCTCTATTATTATAATGAGTCAACAGCAGGGGTTTATTTAATGACTACCTGACTGCCGCTTGGAGCCTAAATGAAAATACCAGAAGAAGATCCTTGAAAGTTTTTGGTTGTTTTCAAGACACAATTATCTGGTGAAATTCTTACGTTTGTATCGTATGTTAAATGTTAAATTGCATTGATAGATTGTGTAATTCATGTTTAATAGTGGCTGCTTTACTTGTTTTTGATTTTTTGTGTGTTTGTGTATTGCACCAATGTTTTTCACTACATTGATGTCGATGATTTATAAATGTTATTGATAGTATTTTTTTGCTGCACAAAGGAGTAACGTGAAATGTGAAATATCACAAAGTTTATTTGTGATTGTGTTTTTTTCTTTTTTACTAAAAGGAGTGATAGTTTTGTGTATGGATAGGATCATTGAAATGATAGTTCATAAGTTTATGAATTTGATCAAATTATGATTTGAATGAGAATAAAATGGAAAGGGCTCCATTTTATAATTGAGTCAACAGCAGGGGTCTGTTTAATGACTACTTGACTGCCGCTTGGAGCCTAAATTAAAATACCAGAAGAAGATCCTTGAAAGTTTTTGGTTGTTTTCAAGACACAATTATCTGGCGAAATCCTTAACATTTGTTTTCTAAATGTAATACTAAAATTATAATTTTATATTTATAGTATATTGTCATAGCTTAAATTAAAATGCCTAAATGGCCCATGTTTTGAGTTGAAACTGTTTTTGTTGAATTCATGTTCCCTGCATGCTAAATCAAGTTTTCTAGCTGCAATTACTATTCTATTTGATACTGGGGGTTGTTTGCTGATTGTTCCTTGTTTTTTTTTTTTTTTTCCTCTTTTCTTATTGACAGCAAGATACACTGTACATGAATATTATAGGATTATTGATATGATTGTTTATAAGTTTTAGGAACTCAAACAAATTATGTTTTTAATGAGAATTAATCTTAAGGGCTCTATTATATTTGAGTCACAGCAGGGGTCTGTTTAATGACTGCCTGACTGCCGCTTGGAGCCTAAACGAAAATACCAGAAGAAGATCCATGAAAACTTTTGGTTGTTTTCAAGTCACAATAATCTGGGGAAATTCTTACATTTGTATCATATCTTAAATGTTAAAGATATTTTAAATGCATATTTGATTTTTCATACACGCCAATTTATTTTTATTATTTTGCCTTGAAAATTGGTTGACGATATGGAATGATTATTTATTCTTGAATGATAAAATGTGTAAATTCGACTTGTTTTTAGCTGAAATAGTTTGTTTTGCTTTCATGCTAAGAAATCAAGTCTAGTGCAATCACCGATCGTCAAGTTTTCATATCTATTAAATGCTGGGCCAGTTAACTGATTTTCGTTTTTTTTCTTTTTGGCAGCTAGATACACCGTCCGTTCTTTTGGTATTAGAAGAAATGAAAAGATTGCTTGCTATGTGACTGTTAGAGGTGACAAGGCAATGCAGCTTTTGGAGAGTGGTTTGAAGGTGAAGGAATATGAATTGCTGAGAAGAAATTTTAGTGATACCGGTTGTTTTGGATTCGGTATTCAGGAGCATATTGATCTGGGAATCAAGTAAGCTCTAAATCTTTTGTATCTGTGACTAAAGTATATGTTGCACGTTATACTTATTATGTGCCATGTGAAACTGGTATAAACAAACTTTTCTAGAACGTGCCATGTGAAACTGTTTTAAACAAACTTTTCTAGAACTGCGTTATTTATGTTTCATTTATCTACTAATGATTCAGTTCTTCAAGAGGAAATTATGGCTAAAGCTATCAGGTGTTCAAAGTTTCGTTGTAGTGCTTGTAGCTCATCCCTAATTGAGCTGCACATGGGAGAAAAAGTTTTACATAATTCTAGGATAGTTGTTTATCACAATGCATGTTGGATTTGAAGTTAGCTGAGTTAATCCGATTTTTACGTCTCTTTAAGCTTATTATGAAAATTTGAAATTCAGTCTTTGGTTTCCTTATCATTTCAGTCTTTAAGCTTATAATCCGATTTCTGATGATATTCCATCACCACCAAGTTTGTTATGATTTATGTTTAACATTGTTTCTTTCTCAGGTATGATCCTTCAACTGGTATTTATGGAATGGACTTTTTCGTTGTTCTGGAGCGTCCCGGTTACCGTGTGGGACGTCGTCGTCGGTGCAAGGCTCGTGTTGGGATCCAACATCGCGTAACAAAGGACGATGCCATGAAGTGGTTCCAGGTGAAATATGAAGGTGTTATCCTCAACAAGTCACAGGCGATTGTTTAG
Protein sequence

>MS.gene066933.t1

MAQQQPLFRFQPQMVSEKKLSNTMRDIKVQKLVLNISVGESGDRLTRAAKVLEQLSGQTPVFSKARYTVRSFGIRRNEKIACYVTVRGDKAMQLLESGLKVKEYELLRRNFSDTGCFGFGIQEHIDLGIKYDPSTGIYGMDFFVVLERPGYRVGRRRRCKARVGIQHRVTKDDAMKWFQVKYEGVILNKSQAIV