AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047421

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047421.t1 MTR_2g008870 93.656 331 19 2 1 329 1 331 0.0 625
MS.gene047421.t1 MTR_2g008870 94.718 284 14 1 1 283 1 284 0.0 552
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047421.t1 AT2G37990 62.623 305 110 3 1 302 1 304 1.30e-129 373

Find 72 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCAAAACCTCCTACTAAA+TGG 0.206025 2.4:-74226693 MS.gene047421:CDS
CGATTCTTTCCTTTATGTTT+TGG 0.214740 2.4:+74224385 None:intergenic
TCCCATTTAGTAGGAGGTTT+TGG 0.249068 2.4:+74226691 None:intergenic
GAGTATATCATGATTATTCT+TGG 0.294298 2.4:+74224161 None:intergenic
CTCGGGGTAATCTGGTGGTT+GGG 0.302345 2.4:+74226844 None:intergenic
GTGAAAGAGTGTTTACAAAA+AGG 0.307046 2.4:-74226954 MS.gene047421:CDS
GGTGCGTGGGTTCCTGTTAT+AGG 0.317411 2.4:+74224514 None:intergenic
GTAGGTTTAGCCTCAATAAT+AGG 0.359792 2.4:+74226442 None:intergenic
ATATCCATGTCTGCGTTTCC+AGG 0.400440 2.4:+74226492 None:intergenic
CCTTCGATTTATCATTCCTT+CGG 0.407735 2.4:+74223987 None:intergenic
GCGACATCTTTGCCTATAAC+AGG 0.413812 2.4:-74224526 MS.gene047421:CDS
CCTTCAACATCTTGAGTAGA+TGG 0.415048 2.4:+74226888 None:intergenic
GGTAATCTGGTGGTTGGGGC+AGG 0.425628 2.4:+74226849 None:intergenic
GCAAACAGGTCCCATTTAGT+AGG 0.428944 2.4:+74226682 None:intergenic
TCTCGGGGTAATCTGGTGGT+TGG 0.429457 2.4:+74226843 None:intergenic
CTACCGGTAGCAGAAGGAAC+AGG 0.433565 2.4:-74224237 MS.gene047421:CDS
CCATCTACTCAAGATGTTGA+AGG 0.442468 2.4:-74226888 MS.gene047421:CDS
GGTTGACCTCGGTAATCTAA+TGG 0.449578 2.4:-74227136 MS.gene047421:CDS
CAAGTTTATGATGAACCATC+TGG 0.452578 2.4:-74226517 MS.gene047421:CDS
GCCAAAACCTCCTACTAAAT+GGG 0.459534 2.4:-74226692 MS.gene047421:CDS
GAAAAGCCTCCAAAACATAA+AGG 0.462069 2.4:-74224394 MS.gene047421:CDS
TCGGGGTAATCTGGTGGTTG+GGG 0.468261 2.4:+74226845 None:intergenic
TTGGCAAAAGGATCCTCGTC+TGG 0.469582 2.4:+74225721 None:intergenic
ACCCCTGTTCCTTCTGCTAC+CGG 0.474967 2.4:+74224234 None:intergenic
AGGAACAGGGGTAGGATCAA+AGG 0.493785 2.4:-74224223 MS.gene047421:CDS
GCCTCAACTGCCACAGCTAG+TGG 0.494544 2.4:-74224445 MS.gene047421:CDS
GGCTGCTAAGAATTTGTTCG+AGG 0.502076 2.4:-74227157 MS.gene047421:CDS
AAGGTTACCAAGGATGAATT+AGG 0.504736 2.4:-74224484 MS.gene047421:CDS
CACAGTGACCAATGTGAAGA+AGG 0.507110 2.4:-74224017 MS.gene047421:CDS
CTGCGTTTCCAGGTTCCAGA+TGG 0.508540 2.4:+74226502 None:intergenic
TACACTGCCTAATTCATCCT+TGG 0.511559 2.4:+74224477 None:intergenic
GATGAATTAGGCAGTGTAGC+AGG 0.517015 2.4:-74224472 MS.gene047421:CDS
AAGAGAAAAGAAAACAAAAG+AGG 0.517890 2.4:-74225700 MS.gene047421:CDS
ATGATGAACCATCTGGAACC+TGG 0.518041 2.4:-74226510 MS.gene047421:CDS
AACCCACGCACCACAAAAGA+AGG 0.519084 2.4:-74224503 MS.gene047421:CDS
AGAAGCTATTCCTATTATTG+AGG 0.529821 2.4:-74226452 MS.gene047421:CDS
CAAAGTTTGGGGCTTTACCT+AGG 0.530560 2.4:-74225627 MS.gene047421:intron
ACCACTAGCTGTGGCAGTTG+AGG 0.531694 2.4:+74224444 None:intergenic
AAGGAAAATGGAGATTGAGA+TGG 0.536528 2.4:-74227181 None:intergenic
CATACCATTCTCAACAATCC+AGG 0.539707 2.4:-74227095 MS.gene047421:intron
GAAAATGGAGATTGAGATGG+AGG 0.541911 2.4:-74227178 None:intergenic
AACAGGTCCCATTTAGTAGG+AGG 0.542260 2.4:+74226685 None:intergenic
ACCATGCAGCCATGTTCAGT+TGG 0.543010 2.4:-74224551 MS.gene047421:intron
ATGTCGCGGCCAACTGAACA+TGG 0.570065 2.4:+74224542 None:intergenic
TCAACTGCCACAGCTAGTGG+TGG 0.576419 2.4:-74224442 MS.gene047421:CDS
TTGGGGCAGGCAATTGAACG+AGG 0.586894 2.4:+74226862 None:intergenic
TGGAATTACTTACCATCGGT+AGG 0.591410 2.4:+74226424 None:intergenic
CATACCTGGATTGTTGAGAA+TGG 0.598739 2.4:+74227091 None:intergenic
GGAACCTGGAAACGCAGACA+TGG 0.610484 2.4:-74226496 MS.gene047421:CDS
TACCGGTAGCAGAAGGAACA+GGG 0.611779 2.4:-74224236 MS.gene047421:CDS
TGGGGCAGGCAATTGAACGA+GGG 0.618140 2.4:+74226863 None:intergenic
AGGGGTAGGATCAAAGGAGA+GGG 0.618168 2.4:-74224217 MS.gene047421:CDS
AGGCATTATTAAGCGGAACA+AGG 0.619070 2.4:-74226545 MS.gene047421:CDS
CAACTGCCACAGCTAGTGGT+GGG 0.621815 2.4:-74224441 MS.gene047421:CDS
TAGTTCCTACCGGTAGCAGA+AGG 0.622035 2.4:-74224243 MS.gene047421:CDS
GTTATGCAGGCATTATTAAG+CGG 0.622663 2.4:-74226552 MS.gene047421:intron
CTGTTCCTTCTGCTACCGGT+AGG 0.633674 2.4:+74224238 None:intergenic
CCGAAGGAATGATAAATCGA+AGG 0.634190 2.4:-74223987 MS.gene047421:CDS
AAACTTCCCACCACTAGCTG+TGG 0.634673 2.4:+74224435 None:intergenic
GTATGAATGATGAGAGTCGA+AGG 0.637332 2.4:+74227113 None:intergenic
AATTTGTTCGAGGTTGACCT+CGG 0.638182 2.4:-74227147 MS.gene047421:CDS
GTAGCAGAAGGAACAGGGGT+AGG 0.639483 2.4:-74224231 MS.gene047421:CDS
GGGGCAGGCAATTGAACGAG+GGG 0.642046 2.4:+74226864 None:intergenic
TGATATACTCAATGTTAACA+AGG 0.643030 2.4:-74224148 MS.gene047421:intron
CAGGGGTAGGATCAAAGGAG+AGG 0.644223 2.4:-74224218 MS.gene047421:CDS
ATTGAGGCTAAACCTACCGA+TGG 0.658675 2.4:-74226436 MS.gene047421:intron
TGTTATAGGCAAAGATGTCG+CGG 0.661140 2.4:+74224528 None:intergenic
TGAAGAAGGAGAAAAGCCGA+AGG 0.662135 2.4:-74224003 MS.gene047421:CDS
ACATAAAGGAAAGAATCGAA+AGG 0.670337 2.4:-74224380 MS.gene047421:intron
ACCACAAAAGAAGGTTACCA+AGG 0.679267 2.4:-74224494 MS.gene047421:CDS
CGAAGGCCATTAGATTACCG+AGG 0.720268 2.4:+74227130 None:intergenic
ACCGGTAGCAGAAGGAACAG+GGG 0.747731 2.4:-74224235 MS.gene047421:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTGTAAATTCTTTA+TGG + chr2.4:74225924-74225943 None:intergenic 10.0%
!! AAACTATTTATAAATGTTAA+GGG - chr2.4:74225185-74225204 MS.gene047421:intron 10.0%
!! AATTAATCTATTCTCTATTA+GGG - chr2.4:74224093-74224112 MS.gene047421:intron 15.0%
!! CAAACTATTTATAAATGTTA+AGG - chr2.4:74225184-74225203 MS.gene047421:intron 15.0%
!! TAATTAATCTATTCTCTATT+AGG - chr2.4:74224092-74224111 MS.gene047421:intron 15.0%
!! TTATTTACAAATCTATAGAT+TGG + chr2.4:74225868-74225887 None:intergenic 15.0%
!! TTGATAAAAATGAGAAAAAT+CGG - chr2.4:74225406-74225425 MS.gene047421:intron 15.0%
!!! ACATTTTAACTTTATCTATA+AGG - chr2.4:74226027-74226046 MS.gene047421:intron 15.0%
!!! ACTTTTGTTATTATTCTTTT+TGG - chr2.4:74225057-74225076 MS.gene047421:intron 15.0%
!!! CTTTTGTTATTATTCTTTTT+GGG - chr2.4:74225058-74225077 MS.gene047421:intron 15.0%
!! AAATTTCTTCTTCAATAAGA+AGG + chr2.4:74224276-74224295 None:intergenic 20.0%
!! AATGAAAAATCAAATTGAAG+TGG + chr2.4:74224042-74224061 None:intergenic 20.0%
!! AATTTCTTCTTCAATAAGAA+GGG + chr2.4:74224275-74224294 None:intergenic 20.0%
!! GTTATTAATAATGAGTTTCT+AGG - chr2.4:74225745-74225764 MS.gene047421:intron 20.0%
!! TCGACAAATAATTATTTACA+AGG + chr2.4:74226761-74226780 None:intergenic 20.0%
!! TGTATACTTACATATGTATT+TGG - chr2.4:74225647-74225666 MS.gene047421:CDS 20.0%
!! TTTGCAAATCTAATTAACAA+AGG + chr2.4:74226100-74226119 None:intergenic 20.0%
!!! ACATTTTAACTTTATCCATA+AGG - chr2.4:74226269-74226288 MS.gene047421:intron 20.0%
!!! ATACGTTTCAATTTTAACTT+TGG - chr2.4:74224799-74224818 MS.gene047421:intron 20.0%
!!! TGGTATTGATTTTTTTTTCT+GGG - chr2.4:74224705-74224724 MS.gene047421:intron 20.0%
! AAAGATCCAACATCTAAATT+AGG - chr2.4:74224911-74224930 MS.gene047421:intron 25.0%
! AAGAGAAAAGAAAACAAAAG+AGG - chr2.4:74225378-74225397 MS.gene047421:intron 25.0%
! AATCATAAGAATAGAACAAG+AGG + chr2.4:74225213-74225232 None:intergenic 25.0%
! ACATAAACAACATCAACAAA+AGG + chr2.4:74224443-74224462 None:intergenic 25.0%
! ACTAGATGAAAAGATAACAA+AGG + chr2.4:74226819-74226838 None:intergenic 25.0%
! AGCATAATATGAGAAAACAA+AGG + chr2.4:74226342-74226361 None:intergenic 25.0%
! GAGTATATCATGATTATTCT+TGG + chr2.4:74226920-74226939 None:intergenic 25.0%
! GATAAAGTTAAAATGTGAAG+TGG + chr2.4:74226265-74226284 None:intergenic 25.0%
! TGATATACTCAATGTTAACA+AGG - chr2.4:74226930-74226949 MS.gene047421:CDS 25.0%
! TGTAAGTATACAAATAGCAA+TGG + chr2.4:74225639-74225658 None:intergenic 25.0%
! TTAGTACACACATGTTAAAT+AGG + chr2.4:74224841-74224860 None:intergenic 25.0%
! TTGTTTATGTTGTCTGATTT+GGG - chr2.4:74224453-74224472 MS.gene047421:CDS 25.0%
!! TTACTGTTTTACAAGTTTGT+TGG - chr2.4:74225602-74225621 MS.gene047421:intron 25.0%
!!! CTGGTATTGATTTTTTTTTC+TGG - chr2.4:74224704-74224723 MS.gene047421:intron 25.0%
!!! GACAAACTAACCTTTATTTT+TGG + chr2.4:74224421-74224440 None:intergenic 25.0%
!!! TATTGATTTTTTTTTCTGGG+TGG - chr2.4:74224708-74224727 MS.gene047421:intron 25.0%
!!! TCTTTTGTTTTCTTTTCTCT+TGG + chr2.4:74225379-74225398 None:intergenic 25.0%
AAGTTTGACAAAAAATTGCC+AGG - chr2.4:74226660-74226679 MS.gene047421:intron 30.0%
AATGAAGCATAGAAACCTTA+TGG + chr2.4:74226287-74226306 None:intergenic 30.0%
AATGTGGAATTACTTACCAT+CGG + chr2.4:74224661-74224680 None:intergenic 30.0%
AATTCCACTGTAATTGTTAC+AGG + chr2.4:74226485-74226504 None:intergenic 30.0%
AATTGCAGTTTAGCCTATAT+AGG - chr2.4:74225799-74225818 MS.gene047421:intron 30.0%
ACATAAAGGAAAGAATCGAA+AGG - chr2.4:74226698-74226717 MS.gene047421:CDS 30.0%
AGAAGCTATTCCTATTATTG+AGG - chr2.4:74224626-74224645 MS.gene047421:intron 30.0%
ATGTAGTGCTATATCGTTAA+TGG - chr2.4:74225314-74225333 MS.gene047421:intron 30.0%
ATTGCAGTTTAGCCTATATA+GGG - chr2.4:74225800-74225819 MS.gene047421:intron 30.0%
CAAAACTGTTAAACAGACTA+AGG + chr2.4:74225968-74225987 None:intergenic 30.0%
CATGCTATGATTAAACATTG+TGG + chr2.4:74224960-74224979 None:intergenic 30.0%
GAAAAATCAAATTGAAGTGG+CGG + chr2.4:74224039-74224058 None:intergenic 30.0%
GTAACAATTACAGTGGAATT+GGG - chr2.4:74226485-74226504 MS.gene047421:CDS 30.0%
GTGTTTATCATTTGCTCATA+AGG - chr2.4:74224338-74224357 MS.gene047421:intron 30.0%
GTTATTATGCTTCAGTGATT+TGG - chr2.4:74226049-74226068 MS.gene047421:intron 30.0%
GTTGTTTATGTTGTCTGATT+TGG - chr2.4:74224452-74224471 MS.gene047421:CDS 30.0%
TAACCAAACATTCAAAACGA+AGG + chr2.4:74224756-74224775 None:intergenic 30.0%
TGAAAAATGCTGCAAAGTTT+GGG - chr2.4:74225439-74225458 MS.gene047421:intron 30.0%
TGTAACAATTACAGTGGAAT+TGG - chr2.4:74226484-74226503 MS.gene047421:CDS 30.0%
TTGAAAAATGCTGCAAAGTT+TGG - chr2.4:74225438-74225457 MS.gene047421:intron 30.0%
! CGATTCTTTCCTTTATGTTT+TGG + chr2.4:74226696-74226715 None:intergenic 30.0%
! TTTTCTTTTCTCTTGGCAAA+AGG + chr2.4:74225372-74225391 None:intergenic 30.0%
!! AGCTATCAATTAGTTTGTAC+AGG - chr2.4:74225768-74225787 MS.gene047421:intron 30.0%
!! CAGAAGAAGCCTTTTAAAAA+GGG - chr2.4:74227143-74227162 MS.gene047421:CDS 30.0%
!! GCTATCAATTAGTTTGTACA+GGG - chr2.4:74225769-74225788 MS.gene047421:intron 30.0%
!! GTGAAAGAGTGTTTACAAAA+AGG - chr2.4:74224124-74224143 MS.gene047421:intron 30.0%
!! TATCCTTCGTTTTGAATGTT+TGG - chr2.4:74224750-74224769 MS.gene047421:intron 30.0%
!! TCAGAAGAAGCCTTTTAAAA+AGG - chr2.4:74227142-74227161 MS.gene047421:CDS 30.0%
!! TTCTTTCCTTTATGTTTTGG+AGG + chr2.4:74226693-74226712 None:intergenic 30.0%
!!! AACCTTTATTTTTGGCAAAC+AGG + chr2.4:74224413-74224432 None:intergenic 30.0%
AAAAAAATCAATACCAGCCC+AGG + chr2.4:74224701-74224720 None:intergenic 35.0%
AAAGTCCTTAGTCTGTTCAA+AGG - chr2.4:74226191-74226210 MS.gene047421:intron 35.0%
AACAATGCATGGATATGTCA+AGG + chr2.4:74225685-74225704 None:intergenic 35.0%
AAGGTTACCAAGGATGAATT+AGG - chr2.4:74226594-74226613 MS.gene047421:intron 35.0%
AATGGTTAAGAGAGTTACCT+AGG + chr2.4:74225471-74225490 None:intergenic 35.0%
AATGTTTGGTTATCGCTGTT+GGG - chr2.4:74224764-74224783 MS.gene047421:intron 35.0%
AATTGTACTCTCTGCATTCT+AGG - chr2.4:74227034-74227053 MS.gene047421:intron 35.0%
ACACAACAGTAGATAACTGT+TGG + chr2.4:74226419-74226438 None:intergenic 35.0%
ACATTGAATTTGACCTCCTT+TGG - chr2.4:74224881-74224900 MS.gene047421:intron 35.0%
ACGGTTCCTAATTTAGATGT+TGG + chr2.4:74224920-74224939 None:intergenic 35.0%
ACTACTCTTGAAAGCTCATA+AGG + chr2.4:74224990-74225009 None:intergenic 35.0%
ATCTTTAAACATGCCAAAGG+AGG + chr2.4:74224897-74224916 None:intergenic 35.0%
ATGATAGCTTCACCCTATAT+AGG + chr2.4:74225815-74225834 None:intergenic 35.0%
CAAAACCTTTGAACAGACTA+AGG + chr2.4:74226199-74226218 None:intergenic 35.0%
CAAGTTTATGATGAACCATC+TGG - chr2.4:74224561-74224580 MS.gene047421:intron 35.0%
CCTTCGATTTATCATTCCTT+CGG + chr2.4:74227094-74227113 None:intergenic 35.0%
GAAAAATGCTGCAAAGTTTG+GGG - chr2.4:74225440-74225459 MS.gene047421:intron 35.0%
GAAAAGCCTCCAAAACATAA+AGG - chr2.4:74226684-74226703 MS.gene047421:CDS 35.0%
GACCTGTTTGCCAAAAATAA+AGG - chr2.4:74224408-74224427 MS.gene047421:CDS 35.0%
GTAGGTTTAGCCTCAATAAT+AGG + chr2.4:74224639-74224658 None:intergenic 35.0%
GTTATGCAGGCATTATTAAG+CGG - chr2.4:74224526-74224545 MS.gene047421:CDS 35.0%
GTTCAACAGAAAACAATGCA+TGG + chr2.4:74225696-74225715 None:intergenic 35.0%
TACACCTGTAACAATTACAG+TGG - chr2.4:74226478-74226497 MS.gene047421:CDS 35.0%
TAGGCTAAACTGCAATTAAG+TGG + chr2.4:74225796-74225815 None:intergenic 35.0%
TCGATAATAGGGATTGAATG+TGG + chr2.4:74224677-74224696 None:intergenic 35.0%
TGGATCTTTAAACATGCCAA+AGG + chr2.4:74224900-74224919 None:intergenic 35.0%
! AAAACCTCAAGGCTAGTTAT+TGG + chr2.4:74225495-74225514 None:intergenic 35.0%
! CTTACTGAACTTTGTTATGC+AGG - chr2.4:74224513-74224532 MS.gene047421:CDS 35.0%
! GGGTACATACATGTTTTTCT+CGG + chr2.4:74224255-74224274 None:intergenic 35.0%
! GGTACATACATGTTTTTCTC+GGG + chr2.4:74224254-74224273 None:intergenic 35.0%
! GTACATACATGTTTTTCTCG+GGG + chr2.4:74224253-74224272 None:intergenic 35.0%
! TATAATGTTTTCAGCTGCCA+AGG - chr2.4:74224364-74224383 MS.gene047421:intron 35.0%
! TCAAGGCTAGTTATTGGAAA+TGG + chr2.4:74225489-74225508 None:intergenic 35.0%
! TTCTTGGCACCCTTTTTAAA+AGG + chr2.4:74227155-74227174 None:intergenic 35.0%
! TTTAACTTTGGATACTTGCG+AGG - chr2.4:74224811-74224830 MS.gene047421:intron 35.0%
!! ATTTCCAATAACTAGCCTTG+AGG - chr2.4:74225488-74225507 MS.gene047421:intron 35.0%
!! TCTTTTCATCTAGTTCCTAC+CGG - chr2.4:74226825-74226844 MS.gene047421:intron 35.0%
!! TTTAAAAAGGGTGCCAAGAA+AGG - chr2.4:74227155-74227174 MS.gene047421:CDS 35.0%
ACCACAAAAGAAGGTTACCA+AGG - chr2.4:74226584-74226603 MS.gene047421:intron 40.0%
AGGCATTATTAAGCGGAACA+AGG - chr2.4:74224533-74224552 MS.gene047421:CDS 40.0%
ATGCATGGATATGTCAAGGT+AGG + chr2.4:74225681-74225700 None:intergenic 40.0%
ATGTTTGGTTATCGCTGTTG+GGG - chr2.4:74224765-74224784 MS.gene047421:intron 40.0%
CAATCCCTATTATCGAACCT+GGG - chr2.4:74224681-74224700 MS.gene047421:intron 40.0%
CATACCATTCTCAACAATCC+AGG - chr2.4:74223983-74224002 MS.gene047421:CDS 40.0%
CATACCTGGATTGTTGAGAA+TGG + chr2.4:74223990-74224009 None:intergenic 40.0%
CCATCTACTCAAGATGTTGA+AGG - chr2.4:74224190-74224209 MS.gene047421:CDS 40.0%
CCGAAGGAATGATAAATCGA+AGG - chr2.4:74227091-74227110 MS.gene047421:intron 40.0%
CCTTCAACATCTTGAGTAGA+TGG + chr2.4:74224193-74224212 None:intergenic 40.0%
GAATGTTTGGTTATCGCTGT+TGG - chr2.4:74224763-74224782 MS.gene047421:intron 40.0%
GACGCGTAGTTACTCATAAT+TGG + chr2.4:74226243-74226262 None:intergenic 40.0%
GCCAAAACCTCCTACTAAAT+GGG - chr2.4:74224386-74224405 MS.gene047421:CDS 40.0%
GGTGGTTGAAAAATGAGACT+TGG - chr2.4:74224726-74224745 MS.gene047421:intron 40.0%
GTATGAATGATGAGAGTCGA+AGG + chr2.4:74223968-74223987 None:intergenic 40.0%
TACACTGCCTAATTCATCCT+TGG + chr2.4:74226604-74226623 None:intergenic 40.0%
TCAATCCCTATTATCGAACC+TGG - chr2.4:74224680-74224699 MS.gene047421:intron 40.0%
TCAGTCAGGACTTCAAAACT+TGG - chr2.4:74225026-74225045 MS.gene047421:intron 40.0%
TGCATGGATATGTCAAGGTA+GGG + chr2.4:74225680-74225699 None:intergenic 40.0%
TGGAATTACTTACCATCGGT+AGG + chr2.4:74224657-74224676 None:intergenic 40.0%
TGTTATAGGCAAAGATGTCG+CGG + chr2.4:74226553-74226572 None:intergenic 40.0%
! AATTTGTTCGAGGTTGACCT+CGG - chr2.4:74223931-74223950 MS.gene047421:CDS 40.0%
! ATGTTTTTCTCGGGGTAATC+TGG + chr2.4:74224245-74224264 None:intergenic 40.0%
! GCTTTTCTCCTTCTTCACAT+TGG + chr2.4:74227072-74227091 None:intergenic 40.0%
! TCCCATTTAGTAGGAGGTTT+TGG + chr2.4:74224390-74224409 None:intergenic 40.0%
!! GGACGATCTGATGCTTTTTA+CGG + chr2.4:74224939-74224958 None:intergenic 40.0%
!! TCCTTGGTAACCTTCTTTTG+TGG + chr2.4:74226588-74226607 None:intergenic 40.0%
!!! AGTTTTGAAGTCCTGACTGA+TGG + chr2.4:74225026-74225045 None:intergenic 40.0%
!!! GTTGTCTGATTTGGGCTTTT+CGG - chr2.4:74224461-74224480 MS.gene047421:CDS 40.0%
!!! GTTTTGAAGTCCTGACTGAT+GGG + chr2.4:74225025-74225044 None:intergenic 40.0%
AACAGGTCCCATTTAGTAGG+AGG + chr2.4:74224396-74224415 None:intergenic 45.0%
ACCATGCAACGAAAACCTCA+AGG + chr2.4:74225506-74225525 None:intergenic 45.0%
ATACATCATCTGCTCGTGCT+TGG - chr2.4:74225551-74225570 MS.gene047421:intron 45.0%
ATATCCATGTCTGCGTTTCC+AGG + chr2.4:74224589-74224608 None:intergenic 45.0%
ATTGAGGCTAAACCTACCGA+TGG - chr2.4:74224642-74224661 MS.gene047421:intron 45.0%
CACAGTGACCAATGTGAAGA+AGG - chr2.4:74227061-74227080 MS.gene047421:intron 45.0%
CACGAGCAGATGATGTATAG+TGG + chr2.4:74225549-74225568 None:intergenic 45.0%
CCATGATTTACCCATCAGTC+AGG - chr2.4:74225012-74225031 MS.gene047421:intron 45.0%
GATGAATTAGGCAGTGTAGC+AGG - chr2.4:74226606-74226625 MS.gene047421:intron 45.0%
GCAAACAGGTCCCATTTAGT+AGG + chr2.4:74224399-74224418 None:intergenic 45.0%
GCGACATCTTTGCCTATAAC+AGG - chr2.4:74226552-74226571 MS.gene047421:intron 45.0%
GGAGTTTGCACATAGTTCAG+AGG + chr2.4:74225947-74225966 None:intergenic 45.0%
GGCCAAAACCTCCTACTAAA+TGG - chr2.4:74224385-74224404 MS.gene047421:CDS 45.0%
GGTTGACCTCGGTAATCTAA+TGG - chr2.4:74223942-74223961 MS.gene047421:CDS 45.0%
TGAAGAAGGAGAAAAGCCGA+AGG - chr2.4:74227075-74227094 MS.gene047421:intron 45.0%
! AACCTTCTTTTGTGGTGCGT+GGG + chr2.4:74226580-74226599 None:intergenic 45.0%
! ACATTTTGCAGATCCAGACG+AGG - chr2.4:74225344-74225363 MS.gene047421:intron 45.0%
! AGAAAGGAGGTGTACATACC+TGG + chr2.4:74224004-74224023 None:intergenic 45.0%
! ATGATGAACCATCTGGAACC+TGG - chr2.4:74224568-74224587 MS.gene047421:intron 45.0%
! CAAAGTTTGGGGCTTTACCT+AGG - chr2.4:74225451-74225470 MS.gene047421:intron 45.0%
! CCTGACTGATGGGTAAATCA+TGG + chr2.4:74225015-74225034 None:intergenic 45.0%
! GGCTGCTAAGAATTTGTTCG+AGG - chr2.4:74223921-74223940 MS.gene047421:CDS 45.0%
! TAACCTTCTTTTGTGGTGCG+TGG + chr2.4:74226581-74226600 None:intergenic 45.0%
! TTTTTCTCGGGGTAATCTGG+TGG + chr2.4:74224242-74224261 None:intergenic 45.0%
!! TTAGTAGGAGGTTTTGGCCT+TGG + chr2.4:74224384-74224403 None:intergenic 45.0%
!!! TGCTTTTGGGTTGCCTTTCT+TGG + chr2.4:74227171-74227190 None:intergenic 45.0%
!!! AAAAAATTGAAAATTTTTTA+TGG + chr2.4:74226156-74226175 None:intergenic 5.0%
AAACTTCCCACCACTAGCTG+TGG + chr2.4:74226646-74226665 None:intergenic 50.0%
AACCCACGCACCACAAAAGA+AGG - chr2.4:74226575-74226594 MS.gene047421:intron 50.0%
ACCAGCCCAGGTTCGATAAT+AGG + chr2.4:74224689-74224708 None:intergenic 50.0%
ACCATGCAGCCATGTTCAGT+TGG - chr2.4:74226527-74226546 MS.gene047421:CDS 50.0%
AGGAACAGGGGTAGGATCAA+AGG - chr2.4:74226855-74226874 MS.gene047421:CDS 50.0%
AGGGGTAGGATCAAAGGAGA+GGG - chr2.4:74226861-74226880 MS.gene047421:CDS 50.0%
CCAGCCCAGGTTCGATAATA+GGG + chr2.4:74224688-74224707 None:intergenic 50.0%
CGAAGGCCATTAGATTACCG+AGG + chr2.4:74223951-74223970 None:intergenic 50.0%
TACCGGTAGCAGAAGGAACA+GGG - chr2.4:74226842-74226861 MS.gene047421:CDS 50.0%
TAGTTCCTACCGGTAGCAGA+AGG - chr2.4:74226835-74226854 MS.gene047421:intron 50.0%
TATCGCTGTTGGGGACAACT+AGG - chr2.4:74224774-74224793 MS.gene047421:intron 50.0%
TTGGCAAAAGGATCCTCGTC+TGG + chr2.4:74225360-74225379 None:intergenic 50.0%
! GCCTTGAGGTTTTCGTTGCA+TGG - chr2.4:74225502-74225521 MS.gene047421:intron 50.0%
!!! TGTTTTGGAGGCTTTTCGCC+TGG + chr2.4:74226681-74226700 None:intergenic 50.0%
ACCACTAGCTGTGGCAGTTG+AGG + chr2.4:74226637-74226656 None:intergenic 55.0%
ACCCCTGTTCCTTCTGCTAC+CGG + chr2.4:74226847-74226866 None:intergenic 55.0%
ACCGGTAGCAGAAGGAACAG+GGG - chr2.4:74226843-74226862 MS.gene047421:CDS 55.0%
ATGTCGCGGCCAACTGAACA+TGG + chr2.4:74226539-74226558 None:intergenic 55.0%
CAACTGCCACAGCTAGTGGT+GGG - chr2.4:74226637-74226656 MS.gene047421:intron 55.0%
CAGGGGTAGGATCAAAGGAG+AGG - chr2.4:74226860-74226879 MS.gene047421:CDS 55.0%
CCCTATTATCGAACCTGGGC+TGG - chr2.4:74224685-74224704 MS.gene047421:intron 55.0%
CTACCGGTAGCAGAAGGAAC+AGG - chr2.4:74226841-74226860 MS.gene047421:CDS 55.0%
CTCGGGGTAATCTGGTGGTT+GGG + chr2.4:74224237-74224256 None:intergenic 55.0%
CTGCGTTTCCAGGTTCCAGA+TGG + chr2.4:74224579-74224598 None:intergenic 55.0%
CTGTTCCTTCTGCTACCGGT+AGG + chr2.4:74226843-74226862 None:intergenic 55.0%
GCCAACTGAACATGGCTGCA+TGG + chr2.4:74226531-74226550 None:intergenic 55.0%
GCGCTCGAAAGGAAGAAAGG+AGG + chr2.4:74224017-74224036 None:intergenic 55.0%
GGAACCTGGAAACGCAGACA+TGG - chr2.4:74224582-74224601 MS.gene047421:intron 55.0%
GGTGCGTGGGTTCCTGTTAT+AGG + chr2.4:74226567-74226586 None:intergenic 55.0%
GTAGCAGAAGGAACAGGGGT+AGG - chr2.4:74226847-74226866 MS.gene047421:CDS 55.0%
TCAACTGCCACAGCTAGTGG+TGG - chr2.4:74226636-74226655 MS.gene047421:intron 55.0%
TCGGGGTAATCTGGTGGTTG+GGG + chr2.4:74224236-74224255 None:intergenic 55.0%
TCTCGGGGTAATCTGGTGGT+TGG + chr2.4:74224238-74224257 None:intergenic 55.0%
TGGGGCAGGCAATTGAACGA+GGG + chr2.4:74224218-74224237 None:intergenic 55.0%
TTGGGGCAGGCAATTGAACG+AGG + chr2.4:74224219-74224238 None:intergenic 55.0%
GCCTCAACTGCCACAGCTAG+TGG - chr2.4:74226633-74226652 MS.gene047421:intron 60.0%
GCGGCGCTCGAAAGGAAGAA+AGG + chr2.4:74224020-74224039 None:intergenic 60.0%
GGGGCAGGCAATTGAACGAG+GGG + chr2.4:74224217-74224236 None:intergenic 60.0%
GGTAATCTGGTGGTTGGGGC+AGG + chr2.4:74224232-74224251 None:intergenic 60.0%
TTGAAGTGGCGGCGCTCGAA+AGG + chr2.4:74224028-74224047 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 74223904 74227196 74223904 ID=MS.gene047421
chr2.4 mRNA 74223904 74227196 74223904 ID=MS.gene047421.t1;Parent=MS.gene047421
chr2.4 exon 74227096 74227196 74227096 ID=MS.gene047421.t1.exon1;Parent=MS.gene047421.t1
chr2.4 CDS 74227096 74227196 74227096 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
chr2.4 exon 74226836 74226986 74226836 ID=MS.gene047421.t1.exon2;Parent=MS.gene047421.t1
chr2.4 CDS 74226836 74226986 74226836 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
chr2.4 exon 74226671 74226722 74226671 ID=MS.gene047421.t1.exon3;Parent=MS.gene047421.t1
chr2.4 CDS 74226671 74226722 74226671 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
chr2.4 exon 74226437 74226565 74226437 ID=MS.gene047421.t1.exon4;Parent=MS.gene047421.t1
chr2.4 CDS 74226437 74226565 74226437 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
chr2.4 exon 74225628 74225745 74225628 ID=MS.gene047421.t1.exon5;Parent=MS.gene047421.t1
chr2.4 CDS 74225628 74225745 74225628 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
chr2.4 exon 74224381 74224564 74224381 ID=MS.gene047421.t1.exon6;Parent=MS.gene047421.t1
chr2.4 CDS 74224381 74224564 74224381 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
chr2.4 exon 74224149 74224262 74224149 ID=MS.gene047421.t1.exon7;Parent=MS.gene047421.t1
chr2.4 CDS 74224149 74224262 74224149 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
chr2.4 exon 74223904 74224044 74223904 ID=MS.gene047421.t1.exon8;Parent=MS.gene047421.t1
chr2.4 CDS 74223904 74224044 74223904 ID=cds.MS.gene047421.t1;Parent=MS.gene047421.t1
Gene Sequence

>MS.gene047421

ATGGAGATTGAGATGGAGGCTGCTAAGAATTTGTTCGAGGTTGACCTCGGTAATCTAATGGCCTTCGACTCTCATCATTCATACCATTCTCAACAATCCAGGTATGTACACCTCCTTTCTTCCTTTCGAGCGCCGCCACTTCAATTTGATTTTTCATTTTGATTTTTCAATCTTTGATACATGTTCTGTAATTAATCTATTCTCTATTAGGGTTGAAGTTGTGAAAGAGTGTTTACAAAAAGGCACTGAGTTAGTTCAAGCTATCGCTGATTCTCTCTTTATTTTACCATCTACTCAAGATGTTGAAGGTCCCCTCGTTCAATTGCCTGCCCCAACCACCAGATTACCCCGAGAAAAACATGTATGTACCCTTCTTATTGAAGAAGAAATTTTATTTATTTATTTATAATATAAACATTTCTTTATTGATTCGTGTGTTTATCATTTGCTCATAAGGTTTTATAATGTTTTCAGCTGCCAAGGCCAAAACCTCCTACTAAATGGGACCTGTTTGCCAAAAATAAAGGTTAGTTTGTCCTTTTGTTGATGTTGTTTATGTTGTCTGATTTGGGCTTTTCGGATTAGACTTTTTTTTTAACTATTTTATTACTTACTGAACTTTGTTATGCAGGCATTATTAAGCGGAACAAGGACAAACAAGTTTATGATGAACCATCTGGAACCTGGAAACGCAGACATGGATATGATCGTGCTAACGATGAAGAAGCTATTCCTATTATTGAGGCTAAACCTACCGATGGTAAGTAATTCCACATTCAATCCCTATTATCGAACCTGGGCTGGTATTGATTTTTTTTTCTGGGTGGTTGAAAAATGAGACTTGGATATCCTTCGTTTTGAATGTTTGGTTATCGCTGTTGGGGACAACTAGGAAATACGTTTCAATTTTAACTTTGGATACTTGCGAGGCAAACCTATTTAACATGTGTGTACTAAAACAGTACAAATTAGAATAGACATTGAATTTGACCTCCTTTGGCATGTTTAAAGATCCAACATCTAAATTAGGAACCGTAAAAAGCATCAGATCGTCCACAATGTTTAATCATAGCATGATAATCACCTTATGAGCTTTCAAGAGTAGTATCCATGATTTACCCATCAGTCAGGACTTCAAAACTTGGAATAAGTTACTTTTGTTATTATTCTTTTTGGGTAATTTGTCAATTTTGTATTTGATTCTGCATTTTTGAGATTATCATTCTTGTGCGTGATAATCTAGATCAAGTACTCTGATTGAAGTTTACTTGTATTGAATTCAAACTATTTATAAATGTTAAGGGAACCTCTTGTTCTATTCTTATGATTTTTCAGAATTATTTTAACATTAAATGTTTTCATGTGCGCATACAAAATTCATACTTGAACTTTGCATTTTTACAGTTTTCTATGTAGTGCTATATCGTTAATGGTATTGTTACATTTTGCAGATCCAGACGAGGATCCTTTTGCCAAGAGAAAAGAAAACAAAAGAGGAAGAGTTGATAAAAATGAGAAAAATCGGATACAGAATTTGAAAAATGCTGCAAAGTTTGGGGCTTTACCTAGGTAACTCTCTTAACCATTTCCAATAACTAGCCTTGAGGTTTTCGTTGCATGGTTGCATTCTATTTATTTCTATCCACTATACATCATCTGCTCGTGCTTGGTGTCATTTATCTGTTGCAATCATAGTGTTTACTGTTTTACAAGTTTGTTGGAGAATTTTGATCCATTGCTATTTGTATACTTACATATGTATTTGGTCTTTTTCCCTACCTTGACATATCCATGCATTGTTTTCTGTTGAACACTTTATCTGAGCTAGTTGTATCTGCAATGTTATTAATAATGAGTTTCTAGGAGCTATCAATTAGTTTGTACAGGGTCCACTTAATTGCAGTTTAGCCTATATAGGGTGAAGCTATCATAAATTGCAGTTGAACATACATTAAGCAACTCCAATCTATAGATTTGTAAATAATCACATGATTTTGTTTCTTTTTGTAATAATTCTCCATAAAGAATTTACAATTTTTTCCTCTGAACTATGTGCAAACTCCTTAGTCTGTTTAACAGTTTTGCAGAAATACTAGTTATGAGTCGCTATACGTCTACTTTAGACATTTTAACTTTATCTATAAGGTTATTATGCTTCAGTGATTTGGAAGTGCGTAAATATGTTTATTAGTTCCTTTGTTAATTAGATTTGCAAATAATCACATGATTGTTTCTTTTGTAAGAATTCTCCATAAAAAATTTTCAATTTTTTCTCTGAACAATGCGAAAAGTCCTTAGTCTGTTCAAAGGTTTTGTATATATCAATGCTAGAAATACCAATTATGAGTAACTACGCGTCCACTTCACATTTTAACTTTATCCATAAGGTTTCTATGCTTCATTAATTCGTAAGTGCATAACTATGTTAATTAGATCCTTTGTTTTCTCATATTATGCTGCAAACAGTCACAGAAGAAGTTGAAAAGAACTGTGTTAATCGTCTAAATAACTACCAACAGTTATCTACTGTTGTGTAACTAGCTGTGACATCACTCTGATTTCTTTGCATTCTTTTACACCTGTAACAATTACAGTGGAATTGGGCTCTAATTCTAAACAATTTACCATGCAGCCATGTTCAGTTGGCCGCGACATCTTTGCCTATAACAGGAACCCACGCACCACAAAAGAAGGTTACCAAGGATGAATTAGGCAGTGTAGCAGGCTTTGCCTCAACTGCCACAGCTAGTGGTGGGAAGTTTGACAAAAAATTGCCAGGCGAAAAGCCTCCAAAACATAAAGGAAAGAATCGAAAGGTGTGAACTGCACTCTTACAGAAATCAGTTTATCTTAACCTTGTAAATAATTATTTGTCGAAACAGTTAACTTTCATTTAATTGACTGATTAGCTTCCTTTGTTATCTTTTCATCTAGTTCCTACCGGTAGCAGAAGGAACAGGGGTAGGATCAAAGGAGAGGGAGCAAACTGAAAATATATTGAACAAGATCATGTCCAAGAATAATCATGATATACTCAATGTTAACAAGGTACATTCAAGTTATTAAGTAACTAGTGACATTGATTTCAATTGTTATAAAGTTTTACTAGCTGTTATTTTTTTCTTTTCATAATTGTACTCTCTGCATTCTAGGCTGTCACAGTGACCAATGTGAAGAAGGAGAAAAGCCGAAGGAATGATAAATCGAAGGCTGCAGCAACTAACAGCAAATTGAAAACTCAGAAGAAGCCTTTTAAAAAGGGTGCCAAGAAAGGCAACCCAAAAGCACAGTAA
Protein sequence

>MS.gene047421.t1

MEIEMEAAKNLFEVDLGNLMAFDSHHSYHSQQSRVEVVKECLQKGTELVQAIADSLFILPSTQDVEGPLVQLPAPTTRLPREKHLPRPKPPTKWDLFAKNKGIIKRNKDKQVYDEPSGTWKRRHGYDRANDEEAIPIIEAKPTDDPDEDPFAKRKENKRGRVDKNEKNRIQNLKNAAKFGALPSHVQLAATSLPITGTHAPQKKVTKDELGSVAGFASTATASGGKFDKKLPGEKPPKHKGKNRKFLPVAEGTGVGSKEREQTENILNKIMSKNNHDILNVNKAVTVTNVKKEKSRRNDKSKAAATNSKLKTQKKPFKKGAKKGNPKAQ