AlfalfaGEDB | MS.gene018045

M. sativa cultivar XinJiangDaYe / MS.gene018045

KEGG: K02868
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00281.22 PF00673.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018045.t1	XP_004293968.1	99.4	181	1	0	1	181	1	181	1.20E-96	362.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018045.t1	P46287	99.4	181	1	0	1	181	1	181	2.8e-99	362.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018045.t1	A0A0S3SAC0	98.9	181	2	0	1	181	1	181	1.9e-96	361.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene058354	MS.gene018045	PPI
MS.gene045454	MS.gene018045	PPI
MS.gene066163	MS.gene018045	PPI
MS.gene040581	MS.gene018045	PPI
MS.gene018045	MS.gene039110	PPI
MS.gene99541	MS.gene018045	PPI
MS.gene018045	MS.gene035331	PPI
MS.gene018045	MS.gene59873	PPI
MS.gene018045	MS.gene010453	PPI
MS.gene018045	MS.gene01172	PPI
MS.gene018045	MS.gene63721	PPI
MS.gene018045	MS.gene031579	PPI
MS.gene018045	MS.gene020400	PPI
MS.gene018045	MS.gene023799	PPI
MS.gene018045	MS.gene52173	PPI
MS.gene018045	MS.gene016301	PPI
MS.gene013036	MS.gene018045	PPI
MS.gene73833	MS.gene018045	PPI
MS.gene018045	MS.gene47347	PPI
MS.gene70551	MS.gene018045	PPI
MS.gene006721	MS.gene018045	PPI
MS.gene42236	MS.gene018045	PPI
MS.gene27648	MS.gene018045	PPI
MS.gene018045	MS.gene002204	PPI
MS.gene047421	MS.gene018045	PPI
MS.gene047734	MS.gene018045	PPI
MS.gene018045	MS.gene060216	PPI
MS.gene018045	MS.gene041557	PPI
MS.gene018045	MS.gene034931	PPI
MS.gene018045	MS.gene071916	PPI
MS.gene066933	MS.gene018045	PPI
MS.gene48447	MS.gene018045	PPI
MS.gene045457	MS.gene018045	PPI
MS.gene018045	MS.gene78700	PPI
MS.gene036487	MS.gene018045	PPI
MS.gene59319	MS.gene018045	PPI
MS.gene063366	MS.gene018045	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018045.t1	MTR_8g046140	98.343	181	3	0	1	181	1	181	1.44e-132	369
MS.gene018045.t1	MTR_4g080740	98.343	181	3	0	1	181	1	181	1.77e-132	368
MS.gene018045.t1	MTR_3g007700	99.441	179	1	0	3	181	4	182	3.84e-132	367
MS.gene018045.t1	MTR_3g007690	80.663	181	33	2	2	180	61	241	1.63e-101	292

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018045.t1	AT2G42740	95.580	181	8	1	181	1	181	4.92e-129	360
MS.gene018045.t1	AT5G45775	95.028	181	9	1	181	1	181	2.14e-128	358
MS.gene018045.t1	AT4G18730	95.028	181	9	1	181	1	181	2.14e-128	358
MS.gene018045.t1	AT3G58700	95.028	181	9	1	181	1	181	2.14e-128	358
MS.gene018045.t1	AT5G45775	95.322	171	8	11	181	1	171	9.31e-121	338

Find 60 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACCCTTCTACTGGTATTTA+TGG	0.148591	7.1:-13586617	MS.gene018045:CDS
TTGCTTTGGATTTGGTATTC+AGG	0.280202	7.1:-13586666	MS.gene018045:CDS
TTCAGTGATACTGGTTGCTT+TGG	0.301811	7.1:-13586680	MS.gene018045:CDS
TATTCAGGAGCATATTGATT+TGG	0.317273	7.1:-13586651	MS.gene018045:CDS
TGACAAGGCAATGCAGCTTT+TGG	0.318529	7.1:-13586747	MS.gene018045:CDS
TGAACCTTGATCTCCCTCAT+TGG	0.332362	7.1:+13586944	None:intergenic
ATTCAGGAGCATATTGATTT+GGG	0.344346	7.1:-13586650	MS.gene018045:CDS
AGGAGGAACTTCAGTGATAC+TGG	0.352064	7.1:-13586689	MS.gene018045:CDS
GAACCTTGATCTCCCTCATT+GGG	0.354507	7.1:+13586945	None:intergenic
ATTCCATAAATACCAGTAGA+AGG	0.360196	7.1:+13586614	None:intergenic
CAATTGCCTGGGATTTGTTA+AGG	0.361161	7.1:+13586453	None:intergenic
GATACTGGTTGCTTTGGATT+TGG	0.362752	7.1:-13586674	MS.gene018045:CDS
TTTGACCTGGAACCACTTCA+TGG	0.374960	7.1:+13586490	None:intergenic
TGATGCCATGAAGTGGTTCC+AGG	0.388963	7.1:-13586495	MS.gene018045:CDS
TTCCAGCACCTTAGCGGCTC+TGG	0.389118	7.1:+13586874	None:intergenic
TGGGTTCGCCAGTTTCTTCT+CGG	0.418287	7.1:+13586964	None:intergenic
AGGTACACTGTGCGATCCTT+TGG	0.421779	7.1:-13586815	MS.gene018045:CDS
CACCAGAGCCGCTAAGGTGC+TGG	0.429975	7.1:-13586876	MS.gene018045:CDS
GGAGAGTGGTTTAAAGGTGA+AGG	0.433325	7.1:-13586726	MS.gene018045:CDS
CGACGACCCACACGGTATCC+AGG	0.444850	7.1:+13586566	None:intergenic
AATGGCTTCCGAGAAGAAAC+TGG	0.445897	7.1:-13586972	MS.gene018045:CDS
TTCTACTGGTATTTATGGAA+TGG	0.448616	7.1:-13586612	MS.gene018045:CDS
ACCTCGCTTTGGAGAACACT+GGG	0.453526	7.1:+13586834	None:intergenic
CTCATTTCTTCTGATTCCAA+AGG	0.457634	7.1:+13586799	None:intergenic
AGTTTAACTAAACAATTGCC+TGG	0.470565	7.1:+13586441	None:intergenic
TCGCACAGTGTACCTCGCTT+TGG	0.474080	7.1:+13586823	None:intergenic
TTTGTTGTTCTGGAGCGCCC+TGG	0.479272	7.1:-13586584	MS.gene018045:CDS
ATCAAGTATGACCCTTCTAC+TGG	0.495573	7.1:-13586626	MS.gene018045:CDS
CAACACGGGCCTTGCATCTG+CGG	0.505355	7.1:+13586543	None:intergenic
AAGTTGTTCCAGCACCTTAG+CGG	0.508576	7.1:+13586868	None:intergenic
GGGTCGTCGCCGCAGATGCA+AGG	0.511173	7.1:-13586552	MS.gene018045:CDS
GTGGTTCCAGGTCAAATATG+AGG	0.522922	7.1:-13586483	MS.gene018045:CDS
AACATCTCCGTCGGTGAGAG+TGG	0.528749	7.1:-13586908	MS.gene018045:CDS
GAACCCAATGAGGGAGATCA+AGG	0.532538	7.1:-13586948	MS.gene018045:CDS
AAGGTGCTGGAACAACTTAG+TGG	0.536530	7.1:-13586863	MS.gene018045:CDS
TCGTCTCACCAGAGCCGCTA+AGG	0.538952	7.1:-13586882	MS.gene018045:CDS
CGCAGATGCAAGGCCCGTGT+TGG	0.542392	7.1:-13586542	MS.gene018045:CDS
GTTTAACTAAACAATTGCCT+GGG	0.556618	7.1:+13586442	None:intergenic
TGGCATCATCCTTCGTGACA+CGG	0.570122	7.1:+13586510	None:intergenic
GAGCGCCCTGGATACCGTGT+GGG	0.587127	7.1:-13586572	MS.gene018045:CDS
CTCGTTCTCAACATCTCCGT+CGG	0.591586	7.1:-13586917	MS.gene018045:CDS
TTCCATAAATACCAGTAGAA+GGG	0.597500	7.1:+13586615	None:intergenic
CACGGTGTTGAATTCCAACA+CGG	0.601715	7.1:+13586528	None:intergenic
CGAAGGATGATGCCATGAAG+TGG	0.606576	7.1:-13586502	MS.gene018045:CDS
GGAGCGCCCTGGATACCGTG+TGG	0.607966	7.1:-13586573	MS.gene018045:CDS
GACGACCCACACGGTATCCA+GGG	0.608386	7.1:+13586567	None:intergenic
AGTGATCCTTAACAAATCCC+AGG	0.615373	7.1:-13586459	MS.gene018045:CDS
TGTTACTGTCAGAGGTGACA+AGG	0.617824	7.1:-13586762	MS.gene018045:CDS
TCACTCCCTCATATTTGACC+TGG	0.621443	7.1:+13586477	None:intergenic
TGGTTCCAGGTCAAATATGA+GGG	0.622773	7.1:-13586482	MS.gene018045:CDS
TACCTCGCTTTGGAGAACAC+TGG	0.628954	7.1:+13586833	None:intergenic
GCATGCTATGTTACTGTCAG+AGG	0.636257	7.1:-13586770	MS.gene018045:CDS
GAAACTGGCGAACCCAATGA+GGG	0.640398	7.1:-13586957	MS.gene018045:CDS
ACGATCTCCACTCTCACCGA+CGG	0.655877	7.1:+13586901	None:intergenic
ATCTGCGGCGACGACCCACA+CGG	0.666808	7.1:+13586558	None:intergenic
CCCCAGTGTTCTCCAAAGCG+AGG	0.672040	7.1:-13586835	MS.gene018045:CDS
AGAAACTGGCGAACCCAATG+AGG	0.672801	7.1:-13586958	MS.gene018045:CDS
ACGGTGTTGAATTCCAACAC+GGG	0.675200	7.1:+13586529	None:intergenic
CCTCGCTTTGGAGAACACTG+GGG	0.688072	7.1:+13586835	None:intergenic
AATTCAACACCGTGTCACGA+AGG	0.700404	7.1:-13586519	MS.gene018045:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATGGATTTTTTTGTTGTTC+TGG	-	chr7.1:13586825-13586844	MS.gene018045:CDS	25.0%
	ATTCCATAAATACCAGTAGA+AGG	+	chr7.1:13586808-13586827	None:intergenic	30.0%
	TTCCATAAATACCAGTAGAA+GGG	+	chr7.1:13586807-13586826	None:intergenic	30.0%
	TTCTACTGGTATTTATGGAA+TGG	-	chr7.1:13586807-13586826	MS.gene018045:CDS	30.0%
!	TATTCAGGAGCATATTGATT+TGG	-	chr7.1:13586768-13586787	MS.gene018045:CDS	30.0%
!!	ATTCAGGAGCATATTGATTT+GGG	-	chr7.1:13586769-13586788	MS.gene018045:CDS	30.0%
	CTCATTTCTTCTGATTCCAA+AGG	+	chr7.1:13586623-13586642	None:intergenic	35.0%
!	TTGCTTTGGATTTGGTATTC+AGG	-	chr7.1:13586753-13586772	MS.gene018045:CDS	35.0%
!!	TGAAGGAGTATGAACTTTTG+AGG	-	chr7.1:13586710-13586729	MS.gene018045:CDS	35.0%
	AGTGATCCTTAACAAATCCC+AGG	-	chr7.1:13586960-13586979	MS.gene018045:CDS	40.0%
	ATCAAGTATGACCCTTCTAC+TGG	-	chr7.1:13586793-13586812	MS.gene018045:CDS	40.0%
	GACCCTTCTACTGGTATTTA+TGG	-	chr7.1:13586802-13586821	MS.gene018045:CDS	40.0%
	TGGTTCCAGGTCAAATATGA+GGG	-	chr7.1:13586937-13586956	MS.gene018045:CDS	40.0%
!	GATACTGGTTGCTTTGGATT+TGG	-	chr7.1:13586745-13586764	MS.gene018045:CDS	40.0%
!	TTCAGTGATACTGGTTGCTT+TGG	-	chr7.1:13586739-13586758	MS.gene018045:CDS	40.0%
!!	AGGAGTATGAACTTTTGAGG+AGG	-	chr7.1:13586713-13586732	MS.gene018045:CDS	40.0%
!!	CAATTGCCTGGGATTTGTTA+AGG	+	chr7.1:13586969-13586988	None:intergenic	40.0%
!!	GCTTTTGGAGAGTGGTTTAA+AGG	-	chr7.1:13586687-13586706	MS.gene018045:CDS	40.0%
	AAGTTGTTCCAGCACCTTAG+CGG	+	chr7.1:13586554-13586573	None:intergenic	45.0%
	AATTCAACACCGTGTCACGA+AGG	-	chr7.1:13586900-13586919	MS.gene018045:CDS	45.0%
	AGGAGGAACTTCAGTGATAC+TGG	-	chr7.1:13586730-13586749	MS.gene018045:CDS	45.0%
	GAACCTTGATCTCCCTCATT+GGG	+	chr7.1:13586477-13586496	None:intergenic	45.0%
	GCATGCTATGTTACTGTCAG+AGG	-	chr7.1:13586649-13586668	MS.gene018045:CDS	45.0%
	GGAGAGTGGTTTAAAGGTGA+AGG	-	chr7.1:13586693-13586712	MS.gene018045:CDS	45.0%
	GTGGTTCCAGGTCAAATATG+AGG	-	chr7.1:13586936-13586955	MS.gene018045:CDS	45.0%
	TCACTCCCTCATATTTGACC+TGG	+	chr7.1:13586945-13586964	None:intergenic	45.0%
	TGAACCTTGATCTCCCTCAT+TGG	+	chr7.1:13586478-13586497	None:intergenic	45.0%
	TGTTACTGTCAGAGGTGACA+AGG	-	chr7.1:13586657-13586676	MS.gene018045:CDS	45.0%
	TTTGACCTGGAACCACTTCA+TGG	+	chr7.1:13586932-13586951	None:intergenic	45.0%
!	ACGGTGTTGAATTCCAACAC+GGG	+	chr7.1:13586893-13586912	None:intergenic	45.0%
!	CACGGTGTTGAATTCCAACA+CGG	+	chr7.1:13586894-13586913	None:intergenic	45.0%
!	TGACAAGGCAATGCAGCTTT+TGG	-	chr7.1:13586672-13586691	MS.gene018045:CDS	45.0%
!!	AAGGTGCTGGAACAACTTAG+TGG	-	chr7.1:13586556-13586575	MS.gene018045:CDS	45.0%
	AGAAACTGGCGAACCCAATG+AGG	-	chr7.1:13586461-13586480	MS.gene018045:CDS	50.0%
	AGGTACACTGTGCGATCCTT+TGG	-	chr7.1:13586604-13586623	MS.gene018045:CDS	50.0%
	CGAAGGATGATGCCATGAAG+TGG	-	chr7.1:13586917-13586936	MS.gene018045:CDS	50.0%
	CTCGTTCTCAACATCTCCGT+CGG	-	chr7.1:13586502-13586521	MS.gene018045:CDS	50.0%
	GAAACTGGCGAACCCAATGA+GGG	-	chr7.1:13586462-13586481	MS.gene018045:CDS	50.0%
	GAACCCAATGAGGGAGATCA+AGG	-	chr7.1:13586471-13586490	MS.gene018045:CDS	50.0%
	TGATGCCATGAAGTGGTTCC+AGG	-	chr7.1:13586924-13586943	MS.gene018045:CDS	50.0%
	TGGCATCATCCTTCGTGACA+CGG	+	chr7.1:13586912-13586931	None:intergenic	50.0%
	TGGGTTCGCCAGTTTCTTCT+CGG	+	chr7.1:13586458-13586477	None:intergenic	50.0%
!!	ACCTCGCTTTGGAGAACACT+GGG	+	chr7.1:13586588-13586607	None:intergenic	50.0%
!!	GCAATGCAGCTTTTGGAGAG+TGG	-	chr7.1:13586679-13586698	MS.gene018045:CDS	50.0%
!!	TACCTCGCTTTGGAGAACAC+TGG	+	chr7.1:13586589-13586608	None:intergenic	50.0%
	AACATCTCCGTCGGTGAGAG+TGG	-	chr7.1:13586511-13586530	MS.gene018045:CDS	55.0%
	ACGATCTCCACTCTCACCGA+CGG	+	chr7.1:13586521-13586540	None:intergenic	55.0%
	TCGCACAGTGTACCTCGCTT+TGG	+	chr7.1:13586599-13586618	None:intergenic	55.0%
!!	CCTCGCTTTGGAGAACACTG+GGG	+	chr7.1:13586587-13586606	None:intergenic	55.0%
!!	TTTGTTGTTCTGGAGCGCCC+TGG	-	chr7.1:13586835-13586854	MS.gene018045:CDS	55.0%
	CAACACGGGCCTTGCATCTG+CGG	+	chr7.1:13586879-13586898	None:intergenic	60.0%
	CCCCAGTGTTCTCCAAAGCG+AGG	-	chr7.1:13586584-13586603	MS.gene018045:CDS	60.0%
	GACGACCCACACGGTATCCA+GGG	+	chr7.1:13586855-13586874	None:intergenic	60.0%
	TCGTCTCACCAGAGCCGCTA+AGG	-	chr7.1:13586537-13586556	MS.gene018045:CDS	60.0%
	TTCCAGCACCTTAGCGGCTC+TGG	+	chr7.1:13586548-13586567	None:intergenic	60.0%
	ATCTGCGGCGACGACCCACA+CGG	+	chr7.1:13586864-13586883	None:intergenic	65.0%
	CACCAGAGCCGCTAAGGTGC+TGG	-	chr7.1:13586543-13586562	MS.gene018045:CDS	65.0%
	CGACGACCCACACGGTATCC+AGG	+	chr7.1:13586856-13586875	None:intergenic	65.0%
	CGCAGATGCAAGGCCCGTGT+TGG	-	chr7.1:13586877-13586896	MS.gene018045:CDS	65.0%
	GAGCGCCCTGGATACCGTGT+GGG	-	chr7.1:13586847-13586866	MS.gene018045:CDS	65.0%
	GGGTCGTCGCCGCAGATGCA+AGG	-	chr7.1:13586867-13586886	MS.gene018045:CDS	70.0%
!	GGAGCGCCCTGGATACCGTG+TGG	-	chr7.1:13586846-13586865	MS.gene018045:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	13586448	13586993	13586448	ID=MS.gene018045
chr7.1	mRNA	13586448	13586993	13586448	ID=MS.gene018045.t1;Parent=MS.gene018045
chr7.1	exon	13586448	13586993	13586448	ID=MS.gene018045.t1.exon1;Parent=MS.gene018045.t1
chr7.1	CDS	13586448	13586993	13586448	ID=cds.MS.gene018045.t1;Parent=MS.gene018045.t1

Gene Sequence

>MS.gene018045

ATGGCTTCCGAGAAGAAACTGGCGAACCCAATGAGGGAGATCAAGGTTCAGAAGCTCGTTCTCAACATCTCCGTCGGTGAGAGTGGAGATCGTCTCACCAGAGCCGCTAAGGTGCTGGAACAACTTAGTGGTCAAACCCCAGTGTTCTCCAAAGCGAGGTACACTGTGCGATCCTTTGGAATCAGAAGAAATGAGAAGATTGCATGCTATGTTACTGTCAGAGGTGACAAGGCAATGCAGCTTTTGGAGAGTGGTTTAAAGGTGAAGGAGTATGAACTTTTGAGGAGGAACTTCAGTGATACTGGTTGCTTTGGATTTGGTATTCAGGAGCATATTGATTTGGGAATCAAGTATGACCCTTCTACTGGTATTTATGGAATGGATTTTTTTGTTGTTCTGGAGCGCCCTGGATACCGTGTGGGTCGTCGCCGCAGATGCAAGGCCCGTGTTGGAATTCAACACCGTGTCACGAAGGATGATGCCATGAAGTGGTTCCAGGTCAAATATGAGGGAGTGATCCTTAACAAATCCCAGGCAATTGTTTAG

Protein sequence

>MS.gene018045.t1

MASEKKLANPMREIKVQKLVLNISVGESGDRLTRAAKVLEQLSGQTPVFSKARYTVRSFGIRRNEKIACYVTVRGDKAMQLLESGLKVKEYELLRRNFSDTGCFGFGIQEHIDLGIKYDPSTGIYGMDFFVVLERPGYRVGRRRRCKARVGIQHRVTKDDAMKWFQVKYEGVILNKSQAIV