Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55165.t1 | XP_003616621.1 | 97.9 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 3.30E-78 | 300.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55165.t1 | Q9ST43 | 70.2 | 141 | 40 | 1 | 4 | 142 | 3 | 143 | 6.8e-56 | 218.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55165.t1 | G7KGZ7 | 97.9 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 2.4e-78 | 300.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene55165 | -0.800268 | 1.56E-48 | -1.69E-46 |
| MS.gene049404 | MS.gene55165 | -0.801143 | 1.03E-48 | -1.69E-46 |
| MS.gene050026 | MS.gene55165 | -0.806374 | 8.48E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene55165 | -0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene55165 | -0.806248 | 9.02E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene55165 | -0.807302 | 5.40E-50 | -1.69E-46 |
| MS.gene051613 | MS.gene55165 | -0.807046 | 6.12E-50 | -1.69E-46 |
| MS.gene052056 | MS.gene55165 | 0.805309 | 1.42E-49 | -1.69E-46 |
| MS.gene052358 | MS.gene55165 | 0.804773 | 1.84E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene55165 | -0.837428 | 5.23E-57 | -1.69E-46 |
| MS.gene052561 | MS.gene55165 | -0.804236 | 2.38E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene55165 | -0.801601 | 8.34E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene55165 | -0.814423 | 1.55E-51 | -1.69E-46 |
| MS.gene052867 | MS.gene55165 | -0.802678 | 5.01E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene55165 | 0.839943 | 1.17E-57 | -1.69E-46 |
| MS.gene053188 | MS.gene55165 | -0.803671 | 3.12E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene55165 | -0.80039 | 1.47E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene55165 | -0.818367 | 2.03E-52 | -1.69E-46 |
| MS.gene05423 | MS.gene55165 | -0.801058 | 1.08E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene55165 | -0.808954 | 2.40E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene55165 | -0.803119 | 4.06E-49 | -1.69E-46 |
| MS.gene055694 | MS.gene55165 | -0.800646 | 1.31E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene55165 | -0.82477 | 6.74E-54 | -1.69E-46 |
| MS.gene055841 | MS.gene55165 | -0.811243 | 7.72E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene55165 | -0.834399 | 3.07E-56 | -1.69E-46 |
| MS.gene056776 | MS.gene55165 | -0.829066 | 6.34E-55 | -1.69E-46 |
| MS.gene057060 | MS.gene55165 | -0.815037 | 1.13E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene55165 | -0.800565 | 1.36E-48 | -1.69E-46 |
| MS.gene057598 | MS.gene55165 | -0.823938 | 1.06E-53 | -1.69E-46 |
| MS.gene057893 | MS.gene55165 | 0.83507 | 2.08E-56 | -1.69E-46 |
| MS.gene058009 | MS.gene55165 | -0.813177 | 2.92E-51 | -1.69E-46 |
| MS.gene058548 | MS.gene55165 | -0.822044 | 2.92E-53 | -1.69E-46 |
| MS.gene058625 | MS.gene55165 | -0.801852 | 7.41E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene55165 | -0.805366 | 1.38E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene55165 | -0.819955 | 8.85E-53 | -1.69E-46 |
| MS.gene05979 | MS.gene55165 | -0.818216 | 2.20E-52 | -1.69E-46 |
| MS.gene060137 | MS.gene55165 | 0.828563 | 8.39E-55 | -1.69E-46 |
| MS.gene060572 | MS.gene55165 | -0.818041 | 2.41E-52 | -1.69E-46 |
| MS.gene060634 | MS.gene55165 | 0.862529 | 4.75E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013786 | MS.gene55165 | PPI |
| MS.gene55165 | MS.gene46264 | PPI |
| MS.gene03201 | MS.gene55165 | PPI |
| MS.gene55165 | MS.gene02572 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55165.t1 | MTR_5g082470 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 1.36e-104 | 295 |
| MS.gene55165.t1 | MTR_3g463520 | 70.922 | 141 | 39 | 1 | 4 | 142 | 3 | 143 | 8.80e-74 | 217 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55165.t1 | AT5G05710 | 80.142 | 141 | 27 | 1 | 3 | 142 | 2 | 142 | 7.64e-81 | 235 |
| MS.gene55165.t1 | AT2G29700 | 70.213 | 141 | 40 | 1 | 4 | 142 | 3 | 143 | 1.03e-71 | 212 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAACCAGCCGGTTCGTTC+TGG | 0.208272 | 5.2:+70437679 | None:intergenic |
| ACTACTCCGGCGTCGAATTC+TGG | 0.239180 | 5.2:-70437708 | MS.gene55165:CDS |
| GCTACTTGTCTCACTGTTAA+AGG | 0.239265 | 5.2:-70437532 | MS.gene55165:CDS |
| TGAAACAAGGGAAACTCTTC+TGG | 0.272043 | 5.2:-70437609 | MS.gene55165:CDS |
| CCGGAATGGCAGCAAGTCTT+TGG | 0.341637 | 5.2:-70437765 | None:intergenic |
| TTAACCAGCCGGTTCGTTCT+GGG | 0.377723 | 5.2:+70437680 | None:intergenic |
| GAAATTGTTGATTATGATAA+CGG | 0.378590 | 5.2:-70437355 | MS.gene55165:CDS |
| TTCTAGATCTGTTACTGATT+CGG | 0.390424 | 5.2:-70437377 | MS.gene55165:CDS |
| GACCGGGTTTGTGGTTGAAC+CGG | 0.413116 | 5.2:+70437732 | None:intergenic |
| AGTCTTTGGCGCTACGCAAC+CGG | 0.436791 | 5.2:-70437751 | MS.gene55165:CDS |
| GAGGATTGGATTAACTCGAT+TGG | 0.460815 | 5.2:-70437418 | MS.gene55165:CDS |
| ACAGTGAGACAAGTAGCAAC+AGG | 0.483239 | 5.2:+70437538 | None:intergenic |
| TGATTCTGATAAGGAGAAAG+AGG | 0.505978 | 5.2:-70437437 | MS.gene55165:CDS |
| CTGATAAGGAGAAAGAGGAT+TGG | 0.515649 | 5.2:-70437432 | MS.gene55165:CDS |
| TGGTCGAACCCAGAACGAAC+CGG | 0.518095 | 5.2:-70437688 | MS.gene55165:CDS |
| GGAGTAGTCGACCGGGTTTG+TGG | 0.532363 | 5.2:+70437723 | None:intergenic |
| ACAAACCCGGTCGACTACTC+CGG | 0.537859 | 5.2:-70437721 | MS.gene55165:CDS |
| TCGACGCCGGAGTAGTCGAC+CGG | 0.566459 | 5.2:+70437715 | None:intergenic |
| AACCGGTTCAACCACAAACC+CGG | 0.568891 | 5.2:-70437734 | MS.gene55165:CDS |
| GTGGAAAGTTCAAACGAGTA+GGG | 0.571418 | 5.2:+70437487 | None:intergenic |
| AGTGGAAAGTTCAAACGAGT+AGG | 0.587441 | 5.2:+70437486 | None:intergenic |
| ATACATCGTATCAGCGCGAG+TGG | 0.592591 | 5.2:+70437468 | None:intergenic |
| CGAACCCAGAACGAACCGGC+TGG | 0.620445 | 5.2:-70437684 | MS.gene55165:CDS |
| CGACGCCGGAGTAGTCGACC+GGG | 0.622012 | 5.2:+70437716 | None:intergenic |
| ACCGGCTGGTTAACAAAACA+AGG | 0.628016 | 5.2:-70437670 | MS.gene55165:CDS |
| GTTCGACCAGAATTCGACGC+CGG | 0.725090 | 5.2:+70437702 | None:intergenic |
| ACACGCGCTTCTATACCGCG+TGG | 0.787665 | 5.2:-70437568 | MS.gene55165:CDS |
| GCAACAGGAATAACACCACG+CGG | 0.811174 | 5.2:+70437553 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTGTTGATTATGATAA+CGG | - | chr5.2:70437753-70437772 | MS.gene55165:CDS | 20.0% |
| !!! | TTTTATTGCTGATTCTGATA+AGG | - | chr5.2:70437662-70437681 | MS.gene55165:CDS | 25.0% |
| AAGGCGAATACATAAAAACA+TGG | - | chr5.2:70437457-70437476 | MS.gene55165:CDS | 30.0% | |
| ! | TTCTAGATCTGTTACTGATT+CGG | - | chr5.2:70437731-70437750 | MS.gene55165:CDS | 30.0% |
| TGATTCTGATAAGGAGAAAG+AGG | - | chr5.2:70437671-70437690 | MS.gene55165:CDS | 35.0% | |
| !!! | GTTGGTTCGTTTTGAAACAA+GGG | - | chr5.2:70437487-70437506 | MS.gene55165:CDS | 35.0% |
| AGTGGAAAGTTCAAACGAGT+AGG | + | chr5.2:70437625-70437644 | None:intergenic | 40.0% | |
| CTGATAAGGAGAAAGAGGAT+TGG | - | chr5.2:70437676-70437695 | MS.gene55165:CDS | 40.0% | |
| GAGGATTGGATTAACTCGAT+TGG | - | chr5.2:70437690-70437709 | MS.gene55165:CDS | 40.0% | |
| GCTACTTGTCTCACTGTTAA+AGG | - | chr5.2:70437576-70437595 | MS.gene55165:CDS | 40.0% | |
| GTGGAAAGTTCAAACGAGTA+GGG | + | chr5.2:70437624-70437643 | None:intergenic | 40.0% | |
| TAAAAACATGGCGAAGACGT+TGG | - | chr5.2:70437469-70437488 | MS.gene55165:CDS | 40.0% | |
| TGAAACAAGGGAAACTCTTC+TGG | - | chr5.2:70437499-70437518 | MS.gene55165:CDS | 40.0% | |
| !!! | CGTTGGTTCGTTTTGAAACA+AGG | - | chr5.2:70437486-70437505 | MS.gene55165:CDS | 40.0% |
| ACAGTGAGACAAGTAGCAAC+AGG | + | chr5.2:70437573-70437592 | None:intergenic | 45.0% | |
| ! | ACCGGCTGGTTAACAAAACA+AGG | - | chr5.2:70437438-70437457 | MS.gene55165:CDS | 45.0% |
| !!! | GCCTTGTTTTGTTAACCAGC+CGG | + | chr5.2:70437442-70437461 | None:intergenic | 45.0% |
| ATACATCGTATCAGCGCGAG+TGG | + | chr5.2:70437643-70437662 | None:intergenic | 50.0% | |
| GCAACAGGAATAACACCACG+CGG | + | chr5.2:70437558-70437577 | None:intergenic | 50.0% | |
| ! | AACCGGTTCAACCACAAACC+CGG | - | chr5.2:70437374-70437393 | MS.gene55165:CDS | 50.0% |
| !! | TTAACCAGCCGGTTCGTTCT+GGG | + | chr5.2:70437431-70437450 | None:intergenic | 50.0% |
| ACAAACCCGGTCGACTACTC+CGG | - | chr5.2:70437387-70437406 | MS.gene55165:CDS | 55.0% | |
| AGTCTTTGGCGCTACGCAAC+CGG | - | chr5.2:70437357-70437376 | MS.gene55165:CDS | 55.0% | |
| GACCGGGTTTGTGGTTGAAC+CGG | + | chr5.2:70437379-70437398 | None:intergenic | 55.0% | |
| GTTCGACCAGAATTCGACGC+CGG | + | chr5.2:70437409-70437428 | None:intergenic | 55.0% | |
| TGGTCGAACCCAGAACGAAC+CGG | - | chr5.2:70437420-70437439 | MS.gene55165:CDS | 55.0% | |
| ! | ACTACTCCGGCGTCGAATTC+TGG | - | chr5.2:70437400-70437419 | MS.gene55165:CDS | 55.0% |
| !! | GTTAACCAGCCGGTTCGTTC+TGG | + | chr5.2:70437432-70437451 | None:intergenic | 55.0% |
| ACACGCGCTTCTATACCGCG+TGG | - | chr5.2:70437540-70437559 | MS.gene55165:CDS | 60.0% | |
| GGAGTAGTCGACCGGGTTTG+TGG | + | chr5.2:70437388-70437407 | None:intergenic | 60.0% | |
| CGAACCCAGAACGAACCGGC+TGG | - | chr5.2:70437424-70437443 | MS.gene55165:CDS | 65.0% | |
| TCGACGCCGGAGTAGTCGAC+CGG | + | chr5.2:70437396-70437415 | None:intergenic | 65.0% | |
| CGACGCCGGAGTAGTCGACC+GGG | + | chr5.2:70437395-70437414 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 70437348 | 70437782 | 70437348 | ID=MS.gene55165 |
| chr5.2 | mRNA | 70437348 | 70437782 | 70437348 | ID=MS.gene55165.t1;Parent=MS.gene55165 |
| chr5.2 | exon | 70437348 | 70437782 | 70437348 | ID=MS.gene55165.t1.exon1;Parent=MS.gene55165.t1 |
| chr5.2 | CDS | 70437348 | 70437782 | 70437348 | ID=cds.MS.gene55165.t1;Parent=MS.gene55165.t1 |
| Gene Sequence |
| Protein sequence |
