AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59616


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59616.t1 MTR_8g028180 98.630 146 2 0 1 146 1 146 3.24e-101 286
MS.gene59616.t1 MTR_3g005520 83.333 150 21 1 1 146 1 150 2.32e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59616.t1 AT2G04520 87.671 146 17 1 1 146 1 145 8.27e-85 245
MS.gene59616.t1 AT5G35680 86.986 146 18 1 1 146 1 145 7.68e-80 232
MS.gene59616.t1 AT5G35680 86.986 146 18 1 1 146 1 145 7.68e-80 232
MS.gene59616.t1 AT5G35680 93.277 119 8 0 1 119 1 119 6.39e-78 228

Find 49 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGATATTGATAAGATTTA+AGG 0.166122 8.1:+73596335 MS.gene59616:CDS
TGTTGGGTTGCGTGATTATC+AGG 0.308178 8.1:+73596148 MS.gene59616:CDS
CCTCCCTTTCCCTTGTTCTT+CGG 0.331257 8.1:-73595918 None:intergenic
GCTGGGGATATTATTCTTGT+TGG 0.331915 8.1:+73596131 MS.gene59616:CDS
TCATTAAGCCTCACACCTTC+CGG 0.357600 8.1:-73596242 None:intergenic
GGAATTGGTGGTGGGATTGA+TGG 0.360212 8.1:+73596266 MS.gene59616:CDS
CTGGGGATATTATTCTTGTT+GGG 0.363705 8.1:+73596132 MS.gene59616:CDS
GAAAGATGCACAAGAAGGTT+TGG 0.408452 8.1:+73596102 MS.gene59616:CDS
AGGATATTGATAAGATTTAA+GGG 0.414173 8.1:+73596336 MS.gene59616:CDS
CTTTCCCTTGTTCTTCGGCA+TGG 0.425552 8.1:-73595913 None:intergenic
GAATTGGTGGTGGGATTGAT+GGG 0.434528 8.1:+73596267 MS.gene59616:CDS
GAAGGCGTATAGCGAGCTTC+CGG 0.446342 8.1:+73596223 MS.gene59616:CDS
AAGAAGGTTTGGATTGCTGC+TGG 0.450047 8.1:+73596113 MS.gene59616:CDS
TTCAACAACCTCGCCTCATC+CGG 0.452149 8.1:-73596203 None:intergenic
TTATATTGAGTTTGAGGATG+AGG 0.463979 8.1:+73596316 MS.gene59616:CDS
GCACAGGTTCTTCGTATGCT+TGG 0.465282 8.1:+73596020 MS.gene59616:CDS
GGGGATGATGATGAGGGTGA+TGG 0.471229 8.1:+73596287 MS.gene59616:CDS
GGGATGATGATGAGGGTGAT+GGG 0.477444 8.1:+73596288 MS.gene59616:CDS
GAATGATTATATTGAGTTTG+AGG 0.491210 8.1:+73596310 MS.gene59616:CDS
GTGAGGCTTAATGAAGGAAT+TGG 0.513375 8.1:+73596251 MS.gene59616:CDS
CTTAATGAAGGAATTGGTGG+TGG 0.513624 8.1:+73596257 MS.gene59616:CDS
GAAGGTTTGGATTGCTGCTG+GGG 0.517248 8.1:+73596115 MS.gene59616:CDS
GCGTGATTATCAGGATGATA+AGG 0.518535 8.1:+73596157 MS.gene59616:CDS
GGATGAGGCGAGGTTGTTGA+AGG 0.519284 8.1:+73596205 MS.gene59616:CDS
AGAAGGTTTGGATTGCTGCT+GGG 0.519846 8.1:+73596114 MS.gene59616:CDS
CCTTCTTGTGCATCTTTCCA+CGG 0.526928 8.1:-73596097 None:intergenic
ATGCCGAAGAACAAGGGAAA+GGG 0.538255 8.1:+73595915 MS.gene59616:CDS
GATTGATGGGGATGATGATG+AGG 0.545936 8.1:+73596280 MS.gene59616:CDS
TTCAACCATGCCGAAGAACA+AGG 0.546102 8.1:+73595908 None:intergenic
GTTCTTCGTATGCTTGGAAA+CGG 0.546331 8.1:+73596026 MS.gene59616:CDS
CTTGAAGTATATGCCGGATG+AGG 0.557673 8.1:+73596190 MS.gene59616:CDS
ATTGATGGGGATGATGATGA+GGG 0.568262 8.1:+73596281 MS.gene59616:CDS
CTTTGTTCCATCGATACACA+TGG 0.573074 8.1:-73596060 None:intergenic
GGAGGAAAGAATCGCAAGAG+AGG 0.578624 8.1:+73595936 MS.gene59616:CDS
CCGTGGAAAGATGCACAAGA+AGG 0.600822 8.1:+73596097 MS.gene59616:CDS
CATGCCGAAGAACAAGGGAA+AGG 0.602323 8.1:+73595914 None:intergenic
TTAATGAAGGAATTGGTGGT+GGG 0.613970 8.1:+73596258 MS.gene59616:CDS
AAGCGTCTCTGTCATATCCG+TGG 0.616715 8.1:+73596080 MS.gene59616:CDS
GCGAGCTTCCGGAAGGTGTG+AGG 0.621721 8.1:+73596234 MS.gene59616:CDS
GAAGGTGTGAGGCTTAATGA+AGG 0.623962 8.1:+73596245 MS.gene59616:CDS
TGTGAAGCCATGTGTATCGA+TGG 0.627821 8.1:+73596053 MS.gene59616:CDS
CCGAAGAACAAGGGAAAGGG+AGG 0.634335 8.1:+73595918 MS.gene59616:CDS
AATTGGTGGTGGGATTGATG+GGG 0.634571 8.1:+73596268 MS.gene59616:CDS
AGGCTTAATGAAGGAATTGG+TGG 0.634691 8.1:+73596254 MS.gene59616:CDS
TCAACCATGCCGAAGAACAA+GGG 0.635918 8.1:+73595909 None:intergenic
GCGTATAGCGAGCTTCCGGA+AGG 0.641188 8.1:+73596227 MS.gene59616:CDS
AGATGGTCAAGAATACGCAC+AGG 0.647023 8.1:+73596004 MS.gene59616:CDS
TGTGATCTTGAAGTATATGC+CGG 0.651550 8.1:+73596184 MS.gene59616:CDS
AGTATATGCCGGATGAGGCG+AGG 0.665508 8.1:+73596195 MS.gene59616:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATGATTATATTGAGTTTG+AGG + chr8.1:73596310-73596329 MS.gene59616:CDS 25.0%
TTATATTGAGTTTGAGGATG+AGG + chr8.1:73596316-73596335 MS.gene59616:CDS 30.0%
CTGGGGATATTATTCTTGTT+GGG + chr8.1:73596132-73596151 MS.gene59616:CDS 35.0%
TGTGATCTTGAAGTATATGC+CGG + chr8.1:73596184-73596203 MS.gene59616:CDS 35.0%
! TTAATGAAGGAATTGGTGGT+GGG + chr8.1:73596258-73596277 MS.gene59616:CDS 35.0%
AGGCTTAATGAAGGAATTGG+TGG + chr8.1:73596254-73596273 MS.gene59616:CDS 40.0%
CTTAATGAAGGAATTGGTGG+TGG + chr8.1:73596257-73596276 MS.gene59616:CDS 40.0%
CTTTGTTCCATCGATACACA+TGG - chr8.1:73596063-73596082 None:intergenic 40.0%
GAAAGATGCACAAGAAGGTT+TGG + chr8.1:73596102-73596121 MS.gene59616:CDS 40.0%
GCGTGATTATCAGGATGATA+AGG + chr8.1:73596157-73596176 MS.gene59616:CDS 40.0%
GCTGGGGATATTATTCTTGT+TGG + chr8.1:73596131-73596150 MS.gene59616:CDS 40.0%
GTGAGGCTTAATGAAGGAAT+TGG + chr8.1:73596251-73596270 MS.gene59616:CDS 40.0%
GTTCTTCGTATGCTTGGAAA+CGG + chr8.1:73596026-73596045 MS.gene59616:CDS 40.0%
! ATTGATGGGGATGATGATGA+GGG + chr8.1:73596281-73596300 MS.gene59616:CDS 40.0%
!! AAAGCGTGAGCTTGTTTTCA+AGG + chr8.1:73595980-73595999 MS.gene59616:CDS 40.0%
!! GAGCTTGTTTTCAAGGAAGA+TGG + chr8.1:73595987-73596006 MS.gene59616:CDS 40.0%
AAGAAGGTTTGGATTGCTGC+TGG + chr8.1:73596113-73596132 MS.gene59616:CDS 45.0%
AGAAGGTTTGGATTGCTGCT+GGG + chr8.1:73596114-73596133 MS.gene59616:CDS 45.0%
AGATGGTCAAGAATACGCAC+AGG + chr8.1:73596004-73596023 MS.gene59616:CDS 45.0%
ATGCCGAAGAACAAGGGAAA+GGG + chr8.1:73595915-73595934 MS.gene59616:CDS 45.0%
CCTTCTTGTGCATCTTTCCA+CGG - chr8.1:73596100-73596119 None:intergenic 45.0%
CTTGAAGTATATGCCGGATG+AGG + chr8.1:73596190-73596209 MS.gene59616:CDS 45.0%
TCATTAAGCCTCACACCTTC+CGG - chr8.1:73596245-73596264 None:intergenic 45.0%
TGTGAAGCCATGTGTATCGA+TGG + chr8.1:73596053-73596072 MS.gene59616:CDS 45.0%
! AAGCTCACGCTTTTCGTCAT+CGG - chr8.1:73595973-73595992 None:intergenic 45.0%
! GAAGGTGTGAGGCTTAATGA+AGG + chr8.1:73596245-73596264 MS.gene59616:CDS 45.0%
! GATTGATGGGGATGATGATG+AGG + chr8.1:73596280-73596299 MS.gene59616:CDS 45.0%
! TGTTGGGTTGCGTGATTATC+AGG + chr8.1:73596148-73596167 MS.gene59616:CDS 45.0%
!! AATTGGTGGTGGGATTGATG+GGG + chr8.1:73596268-73596287 MS.gene59616:CDS 45.0%
!! GAATTGGTGGTGGGATTGAT+GGG + chr8.1:73596267-73596286 MS.gene59616:CDS 45.0%
AAGCGTCTCTGTCATATCCG+TGG + chr8.1:73596080-73596099 MS.gene59616:CDS 50.0%
CCGTGGAAAGATGCACAAGA+AGG + chr8.1:73596097-73596116 MS.gene59616:CDS 50.0%
CCTCCCTTTCCCTTGTTCTT+CGG - chr8.1:73595921-73595940 None:intergenic 50.0%
GAAGGTTTGGATTGCTGCTG+GGG + chr8.1:73596115-73596134 MS.gene59616:CDS 50.0%
GCACAGGTTCTTCGTATGCT+TGG + chr8.1:73596020-73596039 MS.gene59616:CDS 50.0%
GGAGGAAAGAATCGCAAGAG+AGG + chr8.1:73595936-73595955 MS.gene59616:CDS 50.0%
TTCAACAACCTCGCCTCATC+CGG - chr8.1:73596206-73596225 None:intergenic 50.0%
! GGGATGATGATGAGGGTGAT+GGG + chr8.1:73596288-73596307 MS.gene59616:CDS 50.0%
!! GGAATTGGTGGTGGGATTGA+TGG + chr8.1:73596266-73596285 MS.gene59616:CDS 50.0%
AGTATATGCCGGATGAGGCG+AGG + chr8.1:73596195-73596214 MS.gene59616:CDS 55.0%
CCGAAGAACAAGGGAAAGGG+AGG + chr8.1:73595918-73595937 MS.gene59616:CDS 55.0%
GGATGAGGCGAGGTTGTTGA+AGG + chr8.1:73596205-73596224 MS.gene59616:CDS 55.0%
! GAAGGCGTATAGCGAGCTTC+CGG + chr8.1:73596223-73596242 MS.gene59616:CDS 55.0%
! GGGGATGATGATGAGGGTGA+TGG + chr8.1:73596287-73596306 MS.gene59616:CDS 55.0%
GCGTATAGCGAGCTTCCGGA+AGG + chr8.1:73596227-73596246 MS.gene59616:CDS 60.0%
! GCGAGCTTCCGGAAGGTGTG+AGG + chr8.1:73596234-73596253 MS.gene59616:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 73595915 73596355 73595915 ID=MS.gene59616
chr8.1 mRNA 73595915 73596355 73595915 ID=MS.gene59616.t1;Parent=MS.gene59616
chr8.1 exon 73595915 73596355 73595915 ID=MS.gene59616.t1.exon1;Parent=MS.gene59616.t1
chr8.1 CDS 73595915 73596355 73595915 ID=cds.MS.gene59616.t1;Parent=MS.gene59616.t1
Gene Sequence

>MS.gene59616

ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGCAAGAGAGGAAAGAACGAAGCCGATGACGAAAAGCGTGAGCTTGTTTTCAAGGAAGATGGTCAAGAATACGCACAGGTTCTTCGTATGCTTGGAAACGGTCGTTGTGAAGCCATGTGTATCGATGGAACAAAGCGTCTCTGTCATATCCGTGGAAAGATGCACAAGAAGGTTTGGATTGCTGCTGGGGATATTATTCTTGTTGGGTTGCGTGATTATCAGGATGATAAGGCTGATGTGATCTTGAAGTATATGCCGGATGAGGCGAGGTTGTTGAAGGCGTATAGCGAGCTTCCGGAAGGTGTGAGGCTTAATGAAGGAATTGGTGGTGGGATTGATGGGGATGATGATGAGGGTGATGGGAATGATTATATTGAGTTTGAGGATGAGGATATTGATAAGATTTAA

Protein sequence

>MS.gene59616.t1

MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYSELPEGVRLNEGIGGGIDGDDDEGDGNDYIEFEDEDIDKI