Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93983.t1 | XP_024641403.1 | 97.1 | 69 | 2 | 0 | 4 | 72 | 1 | 69 | 7.90E-28 | 132.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93983.t1 | G7KQA5 | 97.0 | 66 | 2 | 0 | 4 | 69 | 1 | 66 | 4.1e-26 | 126.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene93983 | 0.807152 | 5.81E-50 | -1.69E-46 |
| MS.gene049580 | MS.gene93983 | 0.804323 | 2.28E-49 | -1.69E-46 |
| MS.gene049674 | MS.gene93983 | 0.812889 | 3.38E-51 | -1.69E-46 |
| MS.gene049861 | MS.gene93983 | 0.829042 | 6.42E-55 | -1.69E-46 |
| MS.gene049951 | MS.gene93983 | 0.825443 | 4.68E-54 | -1.69E-46 |
| MS.gene050288 | MS.gene93983 | 0.812559 | 3.99E-51 | -1.69E-46 |
| MS.gene050317 | MS.gene93983 | 0.832117 | 1.13E-55 | -1.69E-46 |
| MS.gene050320 | MS.gene93983 | 0.836143 | 1.11E-56 | -1.69E-46 |
| MS.gene050752 | MS.gene93983 | 0.83462 | 2.70E-56 | -1.69E-46 |
| MS.gene050955 | MS.gene93983 | 0.804031 | 2.63E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene93983 | 0.806308 | 8.76E-50 | -1.69E-46 |
| MS.gene051184 | MS.gene93983 | 0.815346 | 9.69E-52 | -1.69E-46 |
| MS.gene051245 | MS.gene93983 | 0.801103 | 1.05E-48 | -1.69E-46 |
| MS.gene051275 | MS.gene93983 | 0.80315 | 4.00E-49 | -1.69E-46 |
| MS.gene051343 | MS.gene93983 | 0.80933 | 2.00E-50 | -1.69E-46 |
| MS.gene051676 | MS.gene93983 | 0.81502 | 1.14E-51 | -1.69E-46 |
| MS.gene051748 | MS.gene93983 | 0.80937 | 1.96E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene93983 | 0.81455 | 1.46E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene93983 | 0.815728 | 7.97E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene93983 | 0.813265 | 2.79E-51 | -1.69E-46 |
| MS.gene051987 | MS.gene93983 | 0.828227 | 1.01E-54 | -1.69E-46 |
| MS.gene052242 | MS.gene93983 | -0.823021 | 1.73E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene93983 | 0.823198 | 1.58E-53 | -1.69E-46 |
| MS.gene052434 | MS.gene93983 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene052818 | MS.gene93983 | -0.814704 | 1.35E-51 | -1.69E-46 |
| MS.gene053058 | MS.gene93983 | 0.828767 | 7.49E-55 | -1.69E-46 |
| MS.gene053109 | MS.gene93983 | 0.820757 | 5.79E-53 | -1.69E-46 |
| MS.gene053295 | MS.gene93983 | 0.803421 | 3.51E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene93983 | 0.830056 | 3.64E-55 | -1.69E-46 |
| MS.gene053340 | MS.gene93983 | 0.818642 | 1.76E-52 | -1.69E-46 |
| MS.gene05344 | MS.gene93983 | -0.834266 | 3.31E-56 | -1.69E-46 |
| MS.gene053456 | MS.gene93983 | 0.819528 | 1.11E-52 | -1.69E-46 |
| MS.gene053564 | MS.gene93983 | 0.840845 | 6.80E-58 | -1.69E-46 |
| MS.gene053879 | MS.gene93983 | 0.826521 | 2.59E-54 | -1.69E-46 |
| MS.gene053952 | MS.gene93983 | 0.80315 | 4.00E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene93983 | 0.815015 | 1.15E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene93983 | 0.834552 | 2.81E-56 | -1.69E-46 |
| MS.gene054536 | MS.gene93983 | 0.814554 | 1.45E-51 | -1.69E-46 |
| MS.gene054699 | MS.gene93983 | 0.817474 | 3.24E-52 | -1.69E-46 |
| MS.gene05469 | MS.gene93983 | 0.859939 | 2.92E-63 | -1.69E-46 |
| MS.gene055311 | MS.gene93983 | 0.800598 | 1.34E-48 | -1.69E-46 |
| MS.gene056752 | MS.gene93983 | 0.817793 | 2.74E-52 | -1.69E-46 |
| MS.gene056787 | MS.gene93983 | 0.816698 | 4.84E-52 | -1.69E-46 |
| MS.gene056849 | MS.gene93983 | 0.833591 | 4.88E-56 | -1.69E-46 |
| MS.gene056975 | MS.gene93983 | 0.814758 | 1.31E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene93983 | 0.825603 | 4.29E-54 | -1.69E-46 |
| MS.gene056992 | MS.gene93983 | 0.802472 | 5.52E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene93983 | 0.811562 | 6.58E-51 | -1.69E-46 |
| MS.gene057234 | MS.gene93983 | 0.825585 | 4.33E-54 | -1.69E-46 |
| MS.gene057452 | MS.gene93983 | -0.819919 | 9.02E-53 | -1.69E-46 |
| MS.gene057622 | MS.gene93983 | 0.80903 | 2.31E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene93983 | 0.846499 | 2.09E-59 | -1.69E-46 |
| MS.gene057999 | MS.gene93983 | 0.895993 | 5.65E-76 | -1.69E-46 |
| MS.gene058054 | MS.gene93983 | 0.815024 | 1.14E-51 | -1.69E-46 |
| MS.gene058055 | MS.gene93983 | 0.804707 | 1.90E-49 | -1.69E-46 |
| MS.gene058074 | MS.gene93983 | 0.828705 | 7.75E-55 | -1.69E-46 |
| MS.gene058238 | MS.gene93983 | 0.846335 | 2.32E-59 | -1.69E-46 |
| MS.gene059050 | MS.gene93983 | 0.810155 | 1.33E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene93983 | 0.825807 | 3.83E-54 | -1.69E-46 |
| MS.gene059360 | MS.gene93983 | 0.810496 | 1.12E-50 | -1.69E-46 |
| MS.gene059830 | MS.gene93983 | 0.809335 | 1.99E-50 | -1.69E-46 |
| MS.gene059872 | MS.gene93983 | -0.838699 | 2.46E-57 | -1.69E-46 |
| MS.gene06010 | MS.gene93983 | 0.804262 | 2.35E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene93983 | 0.846292 | 2.38E-59 | -1.69E-46 |
| MS.gene06018 | MS.gene93983 | 0.821822 | 3.29E-53 | -1.69E-46 |
| MS.gene060586 | MS.gene93983 | 0.802216 | 6.23E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene93983 | 0.806131 | 9.55E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93983.t1 | MTR_6g071040 | 96.970 | 66 | 2 | 0 | 4 | 69 | 1 | 66 | 6.05e-41 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93983.t1 | AT1G67920 | 54.545 | 66 | 28 | 2 | 7 | 71 | 3 | 67 | 1.83e-11 | 53.9 |
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTCACCGTTGGGAATTTC+AGG | 0.186797 | 6.2:+73598039 | MS.gene93983:CDS |
| CTGAGATAAGACCTGTTCTA+AGG | 0.312109 | 6.2:+73597995 | MS.gene93983:CDS |
| TTCATAACTTGACTATGAAT+TGG | 0.365682 | 6.2:-73597925 | None:intergenic |
| CTTAGAACAGGTCTTATCTC+AGG | 0.409951 | 6.2:-73597994 | None:intergenic |
| GGATGAAGGTGTTGGACAAA+AGG | 0.436976 | 6.2:+73597897 | MS.gene93983:CDS |
| ACGAATTTCGCGGTCACCGT+TGG | 0.444737 | 6.2:+73598029 | MS.gene93983:CDS |
| CGAATTTCGCGGTCACCGTT+GGG | 0.446075 | 6.2:+73598030 | MS.gene93983:CDS |
| CGCGAAATCTCCCTTAGAAC+AGG | 0.449387 | 6.2:-73598006 | None:intergenic |
| AAGAATAACAAGAAGGATGA+AGG | 0.505545 | 6.2:+73597883 | MS.gene93983:CDS |
| GATGAAGGTGTTGGACAAAA+GGG | 0.511350 | 6.2:+73597898 | MS.gene93983:CDS |
| AAAGTATTTGATTTGTCACC+AGG | 0.513116 | 6.2:+73597967 | MS.gene93983:CDS |
| TCAGGCCAAGCTATTTCAGT+TGG | 0.530276 | 6.2:+73598057 | MS.gene93983:CDS |
| AACAAGAAGGATGAAGGTGT+TGG | 0.535489 | 6.2:+73597889 | MS.gene93983:CDS |
| GGTCTTATCTCAGGTTTACC+TGG | 0.537252 | 6.2:-73597985 | None:intergenic |
| AATCACCAACTGAAATAGCT+TGG | 0.541732 | 6.2:-73598062 | None:intergenic |
| TTTCAGTTGGTGATTCATAG+AGG | 0.548698 | 6.2:+73598070 | MS.gene93983:CDS |
| TGAGATAAGACCTGTTCTAA+GGG | 0.567147 | 6.2:+73597996 | MS.gene93983:CDS |
| AGATTTCGCGACGAATTTCG+CGG | 0.567955 | 6.2:+73598019 | MS.gene93983:CDS |
| GAACATGAAGAATAACAAGA+AGG | 0.590935 | 6.2:+73597876 | MS.gene93983:CDS |
| GCTTGGCCTGAAATTCCCAA+CGG | 0.707149 | 6.2:-73598045 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCATAACTTGACTATGAAT+TGG | - | chr6.2:73597928-73597947 | None:intergenic | 25.0% |
| AAGAATAACAAGAAGGATGA+AGG | + | chr6.2:73597883-73597902 | MS.gene93983:CDS | 30.0% | |
| GAACATGAAGAATAACAAGA+AGG | + | chr6.2:73597876-73597895 | MS.gene93983:CDS | 30.0% | |
| !! | AAAGTATTTGATTTGTCACC+AGG | + | chr6.2:73597967-73597986 | MS.gene93983:CDS | 30.0% |
| AATCACCAACTGAAATAGCT+TGG | - | chr6.2:73598065-73598084 | None:intergenic | 35.0% | |
| TGAGATAAGACCTGTTCTAA+GGG | + | chr6.2:73597996-73598015 | MS.gene93983:CDS | 35.0% | |
| CTGAGATAAGACCTGTTCTA+AGG | + | chr6.2:73597995-73598014 | MS.gene93983:CDS | 40.0% | |
| ! | AACAAGAAGGATGAAGGTGT+TGG | + | chr6.2:73597889-73597908 | MS.gene93983:CDS | 40.0% |
| ! | CTTAGAACAGGTCTTATCTC+AGG | - | chr6.2:73597997-73598016 | None:intergenic | 40.0% |
| !! | GATGAAGGTGTTGGACAAAA+GGG | + | chr6.2:73597898-73597917 | MS.gene93983:CDS | 40.0% |
| AGATTTCGCGACGAATTTCG+CGG | + | chr6.2:73598019-73598038 | MS.gene93983:CDS | 45.0% | |
| ! | GGTCTTATCTCAGGTTTACC+TGG | - | chr6.2:73597988-73598007 | None:intergenic | 45.0% |
| ! | TCAGGCCAAGCTATTTCAGT+TGG | + | chr6.2:73598057-73598076 | MS.gene93983:CDS | 45.0% |
| !! | GGATGAAGGTGTTGGACAAA+AGG | + | chr6.2:73597897-73597916 | MS.gene93983:CDS | 45.0% |
| CGCGAAATCTCCCTTAGAAC+AGG | - | chr6.2:73598009-73598028 | None:intergenic | 50.0% | |
| GCTTGGCCTGAAATTCCCAA+CGG | - | chr6.2:73598048-73598067 | None:intergenic | 50.0% | |
| ACGAATTTCGCGGTCACCGT+TGG | + | chr6.2:73598029-73598048 | MS.gene93983:CDS | 55.0% | |
| CGAATTTCGCGGTCACCGTT+GGG | + | chr6.2:73598030-73598049 | MS.gene93983:CDS | 55.0% | |
| CGGTCACCGTTGGGAATTTC+AGG | + | chr6.2:73598039-73598058 | MS.gene93983:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 73597871 | 73598089 | 73597871 | ID=MS.gene93983 |
| chr6.2 | mRNA | 73597871 | 73598089 | 73597871 | ID=MS.gene93983.t1;Parent=MS.gene93983 |
| chr6.2 | exon | 73597871 | 73598089 | 73597871 | ID=MS.gene93983.t1.exon1;Parent=MS.gene93983.t1 |
| chr6.2 | CDS | 73597871 | 73598089 | 73597871 | ID=cds.MS.gene93983.t1;Parent=MS.gene93983.t1 |
| Gene Sequence |
| Protein sequence |
