AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74373


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74373.t1 MTR_8g045300 96.815 157 5 0 1 157 1 157 1.48e-111 313
MS.gene74373.t1 MTR_8g045520 75.641 156 38 0 1 156 1 156 4.01e-86 249
MS.gene74373.t1 MTR_8g045490 70.513 156 45 1 1 156 1 155 1.16e-82 240
MS.gene74373.t1 MTR_8g045570 71.523 151 43 0 1 151 1 151 1.39e-81 238
MS.gene74373.t1 MTR_8g045400 69.231 156 47 1 1 156 1 155 3.86e-79 231
MS.gene74373.t1 MTR_8g045555 68.874 151 47 0 1 151 1 151 7.94e-78 228
MS.gene74373.t1 MTR_8g045695 66.026 156 52 1 1 156 1 155 7.12e-77 226
MS.gene74373.t1 MTR_8g045735 66.452 155 51 1 1 155 1 154 8.10e-75 221
MS.gene74373.t1 MTR_8g045640 63.462 156 56 1 1 156 1 155 4.64e-71 211
MS.gene74373.t1 MTR_8g045665 63.462 156 56 1 1 156 1 155 7.94e-71 210
MS.gene74373.t1 MTR_8g045560 62.821 156 57 1 1 156 1 155 4.63e-70 208
MS.gene74373.t1 MTR_1g030810 42.208 154 86 2 1 152 1 153 8.29e-38 127
MS.gene74373.t1 MTR_1g030820 39.610 154 90 2 1 152 1 153 3.24e-36 122
MS.gene74373.t1 MTR_8g012550 32.667 150 98 3 6 154 8 155 6.61e-26 96.7
MS.gene74373.t1 MTR_1g030840 32.692 156 97 4 1 149 1 155 1.50e-21 85.5
MS.gene74373.t1 MTR_1g031640 34.694 147 87 5 7 151 7 146 8.10e-21 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74373.t1 AT5G28010 32.895 152 99 3 3 152 15 165 1.21e-22 88.6
MS.gene74373.t1 AT5G28010 32.895 152 99 3 3 152 15 165 1.21e-22 88.6
MS.gene74373.t1 AT1G70830 32.237 152 100 3 3 152 22 172 7.23e-22 86.7
MS.gene74373.t1 AT1G70830 32.237 152 100 3 3 152 22 172 1.50e-21 86.7
MS.gene74373.t1 AT1G70840 31.579 152 101 3 3 152 20 170 4.26e-21 84.7
MS.gene74373.t1 AT1G70830 32.237 152 100 3 3 152 22 172 4.30e-21 86.7
MS.gene74373.t1 AT1G70830 32.237 152 100 3 3 152 22 172 1.25e-20 86.7
MS.gene74373.t1 AT1G70830 30.921 152 102 3 3 152 184 334 3.91e-19 82.4
MS.gene74373.t1 AT1G70890 30.921 152 102 3 3 152 7 157 2.25e-20 82.4
MS.gene74373.t1 AT1G70830 30.921 152 102 3 3 152 137 287 1.85e-19 82.8
MS.gene74373.t1 AT1G70850 30.667 150 101 3 3 150 9 157 4.25e-19 80.9
MS.gene74373.t1 AT1G70850 30.667 150 101 3 3 150 9 157 1.01e-18 81.3
MS.gene74373.t1 AT1G70850 30.986 142 95 3 13 152 175 315 1.31e-17 78.2
MS.gene74373.t1 AT1G70850 30.667 150 101 3 3 150 9 157 1.01e-18 81.3
MS.gene74373.t1 AT1G70850 30.986 142 95 3 13 152 175 315 1.31e-17 78.2
MS.gene74373.t1 AT1G23120 28.105 153 105 3 1 153 1 148 1.93e-18 77.0
MS.gene74373.t1 AT1G23120 28.758 153 102 4 3 153 51 198 1.13e-17 76.3
MS.gene74373.t1 AT1G23130 27.152 151 108 2 3 152 10 159 5.89e-17 73.6
MS.gene74373.t1 AT5G28000 28.947 152 105 3 3 152 13 163 1.64e-16 72.4
MS.gene74373.t1 AT1G70870 32.450 151 89 4 1 151 1 138 1.68e-16 72.0
MS.gene74373.t1 AT4G23670 29.605 152 104 3 1 151 1 150 5.89e-16 70.9
MS.gene74373.t1 AT1G70880 25.658 152 110 3 3 152 8 158 3.15e-15 68.9
MS.gene74373.t1 AT1G24020 29.870 154 104 3 1 152 1 152 4.72e-15 68.6
MS.gene74373.t1 AT1G24020 29.870 154 104 3 1 152 1 152 4.72e-15 68.6
MS.gene74373.t1 AT2G01520 27.632 152 107 3 1 151 1 150 1.38e-14 67.4
MS.gene74373.t1 AT1G14930 26.974 152 108 3 1 151 1 150 6.63e-14 65.5
MS.gene74373.t1 AT2G01530 28.289 152 106 3 1 151 1 150 1.68e-13 64.3
MS.gene74373.t1 AT1G35260 27.517 149 98 4 7 150 6 149 6.35e-13 62.8
MS.gene74373.t1 AT1G35310 26.761 142 101 3 13 153 12 151 9.69e-13 62.4
MS.gene74373.t1 AT4G14060 26.974 152 108 3 1 151 1 150 1.25e-12 62.0
MS.gene74373.t1 AT4G23680 25.658 152 110 3 1 151 1 150 2.55e-12 61.2
MS.gene74373.t1 AT1G30990 27.815 151 105 4 1 150 1 148 1.19e-11 59.7
MS.gene74373.t1 AT1G14940 26.490 151 108 3 1 150 1 149 1.41e-11 59.3

Find 21 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAAACTTAGCACTGAAAT+TGG 0.239596 8.4:+56001581 MS.gene74373:CDS
AGGTATTCCATCCAACTATT+TGG 0.260874 8.4:-56002979 None:intergenic
ATGGCACTACTGCAGTTAAA+TGG 0.307509 8.4:+56002917 MS.gene74373:CDS
TTCATCACCTTGATGCAGCT+TGG 0.437287 8.4:-56001690 None:intergenic
GTAAACTTAGCACTGAAATT+GGG 0.446714 8.4:+56001582 MS.gene74373:CDS
AGAAATTGATGAGCACTATA+AGG 0.468901 8.4:+56002858 MS.gene74373:CDS
AAAATCGTCCACAAGATCTT+CGG 0.479675 8.4:+56002832 MS.gene74373:CDS
TATTCCATCCAACTATTTGG+AGG 0.505271 8.4:-56002976 None:intergenic
AGCTGCATCAAGGTGATGAA+TGG 0.510022 8.4:+56001693 MS.gene74373:CDS
TTTCTTCACCGAAGATCTTG+TGG 0.512241 8.4:-56002840 None:intergenic
CATCATTGAAGTGATTGATA+AGG 0.521648 8.4:+56002891 MS.gene74373:CDS
ATGTTGAACCTCCAAATAGT+TGG 0.544310 8.4:+56002968 MS.gene74373:CDS
CGATTTCACAACAGAGGGCA+AGG 0.552186 8.4:+56002762 MS.gene74373:intron
CATGAAACCAAGCTGCATCA+AGG 0.562710 8.4:+56001683 MS.gene74373:CDS
TGAACCTCCAAATAGTTGGA+TGG 0.602941 8.4:+56002972 MS.gene74373:CDS
TGTCTCGAGTGCATTTGCTA+AGG 0.617854 8.4:-56002999 None:intergenic
ACTCTGATTCAGTTAAACAC+TGG 0.620261 8.4:+56001720 MS.gene74373:CDS
TAGTTTGAAGAACTTGTCAG+AGG 0.640528 8.4:-56001615 None:intergenic
TCATCTTGTCAAGCAAGCAA+AGG 0.651550 8.4:+56003029 MS.gene74373:CDS
GAAGTGATTGATAAGGCTGA+TGG 0.663192 8.4:+56002898 MS.gene74373:CDS
AAACACTGGACTCATGTCCG+AGG 0.721608 8.4:+56001734 MS.gene74373:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCCAATAAAAATTAAAA+CGG - chr8.4:56002733-56002752 None:intergenic 10.0%
!!! TGTTTACAATTTTTTTTTTT+GGG + chr8.4:56002567-56002586 MS.gene74373:intron 10.0%
!!! TTGTTTACAATTTTTTTTTT+TGG + chr8.4:56002566-56002585 MS.gene74373:intron 10.0%
!! AATAACATATTGCAAAATTT+GGG + chr8.4:56001973-56001992 MS.gene74373:intron 15.0%
!! ACCATTAAATTAAAATTAGA+TGG + chr8.4:56002243-56002262 MS.gene74373:intron 15.0%
!! AGTATAATTATAGAAACATA+TGG - chr8.4:56002666-56002685 None:intergenic 15.0%
!! ATCATCATGATATATAAAAT+TGG + chr8.4:56002453-56002472 MS.gene74373:intron 15.0%
!! ATTAGAAATATAATTTACCT+CGG - chr8.4:56001754-56001773 None:intergenic 15.0%
!!! AGATGAATATTTTAATTGAA+CGG + chr8.4:56002336-56002355 MS.gene74373:intron 15.0%
!!! ATAACCGTTTTAATTTTTAT+TGG + chr8.4:56002726-56002745 MS.gene74373:intron 15.0%
!!! ATAATTTAAAACTATTTGGT+TGG - chr8.4:56002525-56002544 None:intergenic 15.0%
!!! GTTTACAATTTTTTTTTTTG+GGG + chr8.4:56002568-56002587 MS.gene74373:intron 15.0%
!! AACCAATCAACAATTAATAA+TGG + chr8.4:56002163-56002182 MS.gene74373:intron 20.0%
!! ATTCAAGATCAATATCAATA+TGG - chr8.4:56002105-56002124 None:intergenic 20.0%
!! CAATAACATATTGCAAAATT+TGG + chr8.4:56001972-56001991 MS.gene74373:intron 20.0%
!! GATCTAAGTAAAAGATATAA+AGG + chr8.4:56002060-56002079 MS.gene74373:intron 20.0%
!! TAAAAATATGACAGATCTAA+AGG + chr8.4:56002024-56002043 MS.gene74373:intron 20.0%
!! TTCCATTATTAATTGTTGAT+TGG - chr8.4:56002168-56002187 None:intergenic 20.0%
!!! GACATCTTTAAATTTCATTT+TGG + chr8.4:56001903-56001922 MS.gene74373:intron 20.0%
!!! GCCATCTAATTTTAATTTAA+TGG - chr8.4:56002247-56002266 None:intergenic 20.0%
!!! TATAAAATTGGTTGATTTGA+TGG + chr8.4:56002465-56002484 MS.gene74373:intron 20.0%
!!! TTTAGATCTGTCATATTTTT+AGG - chr8.4:56002025-56002044 None:intergenic 20.0%
! ATTTCTAATAACACTAACAC+TGG + chr8.4:56001765-56001784 MS.gene74373:intron 25.0%
!!! TTGTCTTCTGATTTTTAACT+TGG + chr8.4:56001830-56001849 MS.gene74373:intron 25.0%
AGAAATTGATGAGCACTATA+AGG + chr8.4:56002858-56002877 MS.gene74373:CDS 30.0%
ATGTACAAGTACAACTACAA+AGG + chr8.4:56002365-56002384 MS.gene74373:intron 30.0%
CATCATTGAAGTGATTGATA+AGG + chr8.4:56002891-56002910 MS.gene74373:CDS 30.0%
GATATATATAGCAAGAGTGA+TGG + chr8.4:56002191-56002210 MS.gene74373:intron 30.0%
! GTAAACTTAGCACTGAAATT+GGG + chr8.4:56001582-56001601 MS.gene74373:CDS 30.0%
! TTGATTTGATGGTTGGAATA+GGG + chr8.4:56002476-56002495 MS.gene74373:intron 30.0%
!! AAACTATTTGGTTGGCTTTA+AGG - chr8.4:56002517-56002536 None:intergenic 30.0%
!! AAATTGGTTGATTTGATGGT+TGG + chr8.4:56002469-56002488 MS.gene74373:intron 30.0%
!! GCTTTAAGGATCTCTAATTT+AGG - chr8.4:56002503-56002522 None:intergenic 30.0%
!!! ACTGGATTTATGTGCTATTT+TGG + chr8.4:56001783-56001802 MS.gene74373:intron 30.0%
AAAATCGTCCACAAGATCTT+CGG + chr8.4:56002832-56002851 MS.gene74373:CDS 35.0%
ACTCTGATTCAGTTAAACAC+TGG + chr8.4:56001720-56001739 MS.gene74373:CDS 35.0%
AGGTATTCCATCCAACTATT+TGG - chr8.4:56002982-56003001 None:intergenic 35.0%
ATGTTGAACCTCCAAATAGT+TGG + chr8.4:56002968-56002987 MS.gene74373:CDS 35.0%
TAGTTTGAAGAACTTGTCAG+AGG - chr8.4:56001618-56001637 None:intergenic 35.0%
TATTCCATCCAACTATTTGG+AGG - chr8.4:56002979-56002998 None:intergenic 35.0%
! GGTAAACTTAGCACTGAAAT+TGG + chr8.4:56001581-56001600 MS.gene74373:CDS 35.0%
! TGATTTGATGGTTGGAATAG+GGG + chr8.4:56002477-56002496 MS.gene74373:intron 35.0%
!! ATAGCAAGAGTGATGGATTT+AGG + chr8.4:56002198-56002217 MS.gene74373:intron 35.0%
!! GTTGATTTGATGGTTGGAAT+AGG + chr8.4:56002475-56002494 MS.gene74373:intron 35.0%
ACCTTCGATTTCACAACAGA+GGG + chr8.4:56002757-56002776 MS.gene74373:intron 40.0%
ATGGCACTACTGCAGTTAAA+TGG + chr8.4:56002917-56002936 MS.gene74373:CDS 40.0%
GAAGTGATTGATAAGGCTGA+TGG + chr8.4:56002898-56002917 MS.gene74373:CDS 40.0%
TACCTTCGATTTCACAACAG+AGG + chr8.4:56002756-56002775 MS.gene74373:intron 40.0%
TCATCTTGTCAAGCAAGCAA+AGG + chr8.4:56003029-56003048 MS.gene74373:CDS 40.0%
TGAACCTCCAAATAGTTGGA+TGG + chr8.4:56002972-56002991 MS.gene74373:CDS 40.0%
TTTCTTCACCGAAGATCTTG+TGG - chr8.4:56002843-56002862 None:intergenic 40.0%
AGCTGCATCAAGGTGATGAA+TGG + chr8.4:56001693-56001712 MS.gene74373:CDS 45.0%
CATGAAACCAAGCTGCATCA+AGG + chr8.4:56001683-56001702 MS.gene74373:CDS 45.0%
TTCATCACCTTGATGCAGCT+TGG - chr8.4:56001693-56001712 None:intergenic 45.0%
! TGTCTCGAGTGCATTTGCTA+AGG - chr8.4:56003002-56003021 None:intergenic 45.0%
!!! AATAATAATTTAAAACTATT+TGG - chr8.4:56002529-56002548 None:intergenic 5.0%
!!! TTTTTTTTTAAGAAAAAATT+GGG + chr8.4:56002638-56002657 MS.gene74373:intron 5.0%
!!! TTTTTTTTTTAAGAAAAAAT+TGG + chr8.4:56002637-56002656 MS.gene74373:intron 5.0%
AAACACTGGACTCATGTCCG+AGG + chr8.4:56001734-56001753 MS.gene74373:CDS 50.0%
CGATTTCACAACAGAGGGCA+AGG + chr8.4:56002762-56002781 MS.gene74373:intron 50.0%
GCCCTCTGTTGTGAAATCGA+AGG - chr8.4:56002761-56002780 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 56001569 56003062 56001569 ID=MS.gene74373
chr8.4 mRNA 56001569 56003062 56001569 ID=MS.gene74373.t1;Parent=MS.gene74373
chr8.4 exon 56001569 56001755 56001569 ID=MS.gene74373.t1.exon1;Parent=MS.gene74373.t1
chr8.4 CDS 56001569 56001755 56001569 ID=cds.MS.gene74373.t1;Parent=MS.gene74373.t1
chr8.4 exon 56002776 56003062 56002776 ID=MS.gene74373.t1.exon2;Parent=MS.gene74373.t1
chr8.4 CDS 56002776 56003062 56002776 ID=cds.MS.gene74373.t1;Parent=MS.gene74373.t1
Gene Sequence

>MS.gene74373

ATGGTGCTTGCTGGTAAACTTAGCACTGAAATTGGGATCAAAGCATCCTCTGACAAGTTCTTCAAACTATTCGCATCAAATATTCACGAACTGCAAAACATTTGTGAAAGTGTTCATGAAACCAAGCTGCATCAAGGTGATGAATGGCATCACTCTGATTCAGTTAAACACTGGACTCATGTCCGAGGTAAATTATATTTCTAATAACACTAACACTGGATTTATGTGCTATTTTGGTCTATCAATAAATTTATAATTTATTTGTCTTCTGATTTTTAACTTGGCTAAAGAAAATTTATTTCTCATATTCTTCGAAAAAAATATATTTCTCATAGACATCTTTAAATTTCATTTTGGCAATAACATATTATAAAAAGTTTAATATAACGTAAAAAATAGATGACAATAACATATTGCAAAATTTGGGATATAATTTTTAAAAGAAGATTTAGTCCTAAAAATATGACAGATCTAAAGGTTATATTATGACAGATCTAAGTAAAAGATATAAAGGTTCAAATTGTATAAATATACCATATTGATATTGATCTTGAATTTTGTATAAAACAAACATTTCTCAAGATTTGTTAACAAAACCAATCAACAATTAATAATGGAACAAGATATATATAGCAAGAGTGATGGATTTAGGTTTCAAATAGATGTCATTACTGACCATTAAATTAAAATTAGATGGCTCACGTATCAATTGAAGAGTTTTTGTCAACATACACAATTAATTAAACTCTGAGTCGTTTGATCAAAATAGATGAATATTTTAATTGAACGGTTTGTTATGTACAAGTACAACTACAAAGGATTCTTCTTATACTATAGAAACATAATACATACAGTACATTATATGTCAAAATCAATGATAAATAATCATCATGATATATAAAATTGGTTGATTTGATGGTTGGAATAGGGGCCTAAATTAGAGATCCTTAAAGCCAACCAAATAGTTTTAAATTATTATTATTCATTCAGTTCGATTTTGTTTACAATTTTTTTTTTTGGGGAACAACATATTTTATTTAAGAAATGAAAACAATTTATTATTAACTTTTTTTTTTTTAAGAAAAAATTGGGTTCCATATGTTTCTATAATTATACTTTCATAATTATTCTTTCAAAAAAATGTTTATATAATTATTATAACCGTTTTAATTTTTATTGGAATTAATTACCTTCGATTTCACAACAGAGGGCAAGGTATACACATGTCATGAGAGTTTCGAAGAAATTGATGAAAAGAACAAAAAAATCGTCCACAAGATCTTCGGTGAAGAAATTGATGAGCACTATAAGGTCTTTAAGCTCATCATTGAAGTGATTGATAAGGCTGATGGCACTACTGCAGTTAAATGGACTGTTGAATATGAGAAGATCAATGAAGATGTTGAACCTCCAAATAGTTGGATGGAATACCTTAGCAAATGCACTCGAGACATTGATGCTCATCTTGTCAAGCAAGCAAAGGTTGCTCCATAA

Protein sequence

>MS.gene74373.t1

MVLAGKLSTEIGIKASSDKFFKLFASNIHELQNICESVHETKLHQGDEWHHSDSVKHWTHVREGKVYTCHESFEEIDEKNKKIVHKIFGEEIDEHYKVFKLIIEVIDKADGTTAVKWTVEYEKINEDVEPPNSWMEYLSKCTRDIDAHLVKQAKVAP