Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73327.t1 | XP_024641827.1 | 99.2 | 604 | 5 | 0 | 1 | 604 | 238 | 841 | 0.00E+00 | 1201 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73327.t1 | A0A396HMI3 | 99.2 | 604 | 5 | 0 | 1 | 604 | 238 | 841 | 0.0e+00 | 1201.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene73327 | 0.831042 | 2.09E-55 | -1.69E-46 |
| MS.gene049341 | MS.gene73327 | 0.801897 | 7.25E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene73327 | 0.84208 | 3.22E-58 | -1.69E-46 |
| MS.gene049404 | MS.gene73327 | 0.813265 | 2.79E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene73327 | 0.841703 | 4.05E-58 | -1.69E-46 |
| MS.gene049821 | MS.gene73327 | 0.821345 | 4.24E-53 | -1.69E-46 |
| MS.gene049850 | MS.gene73327 | 0.816874 | 4.42E-52 | -1.69E-46 |
| MS.gene049887 | MS.gene73327 | 0.811991 | 5.31E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene73327 | 0.828431 | 9.03E-55 | -1.69E-46 |
| MS.gene050241 | MS.gene73327 | 0.807623 | 4.62E-50 | -1.69E-46 |
| MS.gene050251 | MS.gene73327 | 0.807355 | 5.26E-50 | -1.69E-46 |
| MS.gene050273 | MS.gene73327 | 0.804776 | 1.84E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene73327 | 0.889852 | 1.67E-73 | -1.69E-46 |
| MS.gene050808 | MS.gene73327 | 0.854616 | 1.09E-61 | -1.69E-46 |
| MS.gene050901 | MS.gene73327 | 0.826559 | 2.54E-54 | -1.69E-46 |
| MS.gene051180 | MS.gene73327 | 0.81696 | 4.22E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene73327 | 0.801066 | 1.07E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene73327 | 0.822109 | 2.82E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene73327 | 0.834377 | 3.11E-56 | -1.69E-46 |
| MS.gene051328 | MS.gene73327 | 0.823162 | 1.61E-53 | -1.69E-46 |
| MS.gene051388 | MS.gene73327 | 0.803981 | 2.69E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene73327 | 0.848354 | 6.46E-60 | -1.69E-46 |
| MS.gene051664 | MS.gene73327 | 0.811445 | 6.98E-51 | -1.69E-46 |
| MS.gene051700 | MS.gene73327 | 0.814331 | 1.63E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene73327 | 0.879745 | 9.70E-70 | -1.69E-46 |
| MS.gene051720 | MS.gene73327 | 0.889899 | 1.60E-73 | -1.69E-46 |
| MS.gene052020 | MS.gene73327 | 0.809215 | 2.11E-50 | -1.69E-46 |
| MS.gene052133 | MS.gene73327 | 0.848624 | 5.44E-60 | -1.69E-46 |
| MS.gene052397 | MS.gene73327 | 0.803801 | 2.93E-49 | -1.69E-46 |
| MS.gene052514 | MS.gene73327 | 0.808213 | 3.46E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene73327 | 0.870271 | 1.66E-66 | -1.69E-46 |
| MS.gene052665 | MS.gene73327 | 0.862026 | 6.78E-64 | -1.69E-46 |
| MS.gene052732 | MS.gene73327 | 0.848668 | 5.29E-60 | -1.69E-46 |
| MS.gene053079 | MS.gene73327 | 0.808196 | 3.49E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene73327 | 0.865636 | 5.11E-65 | -1.69E-46 |
| MS.gene053174 | MS.gene73327 | 0.828316 | 9.62E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene73327 | 0.83631 | 1.01E-56 | -1.69E-46 |
| MS.gene053563 | MS.gene73327 | 0.819084 | 1.40E-52 | -1.69E-46 |
| MS.gene053581 | MS.gene73327 | 0.866948 | 1.96E-65 | -1.69E-46 |
| MS.gene053599 | MS.gene73327 | 0.829121 | 6.15E-55 | -1.69E-46 |
| MS.gene05364 | MS.gene73327 | 0.828726 | 7.66E-55 | -1.69E-46 |
| MS.gene054550 | MS.gene73327 | 0.834862 | 2.34E-56 | -1.69E-46 |
| MS.gene054584 | MS.gene73327 | 0.87056 | 1.33E-66 | -1.69E-46 |
| MS.gene054710 | MS.gene73327 | 0.815216 | 1.04E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene73327 | 0.804907 | 1.72E-49 | -1.69E-46 |
| MS.gene054791 | MS.gene73327 | 0.820335 | 7.24E-53 | -1.69E-46 |
| MS.gene055144 | MS.gene73327 | 0.84117 | 5.59E-58 | -1.69E-46 |
| MS.gene055465 | MS.gene73327 | 0.878185 | 3.44E-69 | -1.69E-46 |
| MS.gene055466 | MS.gene73327 | 0.870251 | 1.68E-66 | -1.69E-46 |
| MS.gene055467 | MS.gene73327 | 0.824354 | 8.45E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene73327 | 0.874728 | 5.38E-68 | -1.69E-46 |
| MS.gene055469 | MS.gene73327 | 0.867561 | 1.25E-65 | -1.69E-46 |
| MS.gene055512 | MS.gene73327 | 0.826861 | 2.15E-54 | -1.69E-46 |
| MS.gene055537 | MS.gene73327 | 0.864025 | 1.64E-64 | -1.69E-46 |
| MS.gene055580 | MS.gene73327 | -0.844476 | 7.38E-59 | -1.69E-46 |
| MS.gene055890 | MS.gene73327 | 0.847717 | 9.69E-60 | -1.69E-46 |
| MS.gene055891 | MS.gene73327 | 0.867406 | 1.40E-65 | -1.69E-46 |
| MS.gene055926 | MS.gene73327 | 0.824925 | 6.20E-54 | -1.69E-46 |
| MS.gene056005 | MS.gene73327 | 0.83848 | 2.81E-57 | -1.69E-46 |
| MS.gene056043 | MS.gene73327 | -0.831196 | 1.91E-55 | -1.69E-46 |
| MS.gene05604 | MS.gene73327 | 0.803598 | 3.23E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene73327 | 0.801858 | 7.38E-49 | -1.69E-46 |
| MS.gene056136 | MS.gene73327 | 0.80432 | 2.29E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene73327 | 0.855669 | 5.40E-62 | -1.69E-46 |
| MS.gene056507 | MS.gene73327 | 0.820955 | 5.22E-53 | -1.69E-46 |
| MS.gene056553 | MS.gene73327 | 0.822629 | 2.14E-53 | -1.69E-46 |
| MS.gene056643 | MS.gene73327 | 0.800114 | 1.68E-48 | -1.69E-46 |
| MS.gene057444 | MS.gene73327 | 0.817194 | 3.74E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene73327 | 0.807982 | 3.87E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene73327 | 0.800386 | 1.48E-48 | -1.69E-46 |
| MS.gene058087 | MS.gene73327 | 0.823333 | 1.47E-53 | -1.69E-46 |
| MS.gene058330 | MS.gene73327 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene73327 | 0.827908 | 1.21E-54 | -1.69E-46 |
| MS.gene05847 | MS.gene73327 | 0.81134 | 7.35E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene73327 | 0.819666 | 1.03E-52 | -1.69E-46 |
| MS.gene058647 | MS.gene73327 | 0.800785 | 1.22E-48 | -1.69E-46 |
| MS.gene058668 | MS.gene73327 | 0.809669 | 1.69E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene73327 | 0.835502 | 1.62E-56 | -1.69E-46 |
| MS.gene058945 | MS.gene73327 | 0.822121 | 2.81E-53 | -1.69E-46 |
| MS.gene05894 | MS.gene73327 | 0.813195 | 2.89E-51 | -1.69E-46 |
| MS.gene059171 | MS.gene73327 | 0.807731 | 4.38E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene73327 | 0.807692 | 4.46E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene73327 | 0.815245 | 1.02E-51 | -1.69E-46 |
| MS.gene05978 | MS.gene73327 | 0.801208 | 1.00E-48 | -1.69E-46 |
| MS.gene059842 | MS.gene73327 | 0.800576 | 1.35E-48 | -1.69E-46 |
| MS.gene059937 | MS.gene73327 | 0.835995 | 1.21E-56 | -1.69E-46 |
| MS.gene060519 | MS.gene73327 | 0.850175 | 2.01E-60 | -1.69E-46 |
| MS.gene060587 | MS.gene73327 | 0.863531 | 2.33E-64 | -1.69E-46 |
| MS.gene060603 | MS.gene73327 | 0.813379 | 2.64E-51 | -1.69E-46 |
| MS.gene061006 | MS.gene73327 | 0.829417 | 5.21E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73327.t1 | AT1G62870 | 59.106 | 604 | 217 | 7 | 1 | 601 | 183 | 759 | 0.0 | 723 |
| MS.gene73327.t1 | AT1G12380 | 58.279 | 616 | 221 | 6 | 1 | 601 | 196 | 790 | 0.0 | 723 |
Find 117 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGAGTTTGGTTAAGTTTA+TGG | 0.125038 | 6.4:-44135358 | MS.gene73327:CDS |
| GGCTAAGGCATTGAGGAATT+TGG | 0.155802 | 6.4:-44135175 | MS.gene73327:CDS |
| TAGGTCGATGTTTGCCTCTT+TGG | 0.199962 | 6.4:-44134681 | MS.gene73327:CDS |
| GATTGTTGCTGATAAGTTTA+AGG | 0.205077 | 6.4:-44135196 | MS.gene73327:CDS |
| TCGCTTGTGAAGATTATTAA+AGG | 0.212044 | 6.4:-44134739 | MS.gene73327:CDS |
| GTGAATCCGAGGCGAAAATT+AGG | 0.220507 | 6.4:-44135485 | MS.gene73327:CDS |
| TCTTTCATGAGAAAAGTTTC+TGG | 0.240470 | 6.4:-44134172 | MS.gene73327:CDS |
| TGTGATGGAGGACGCGTTTA+AGG | 0.241698 | 6.4:-44134848 | MS.gene73327:CDS |
| TTGTGTGAGTTTAAATCTTT+AGG | 0.280903 | 6.4:-44134256 | MS.gene73327:CDS |
| TTTGAAAAGGCGGTGTTTAC+CGG | 0.292873 | 6.4:-44135309 | MS.gene73327:CDS |
| TGTTTGAGACGCGAGGTTTC+CGG | 0.293572 | 6.4:-44135543 | MS.gene73327:CDS |
| CTTTGGATTTACTTGCTGAT+TGG | 0.300028 | 6.4:-44135659 | MS.gene73327:CDS |
| CTCTTAGTAGTCGACTTGTT+TGG | 0.307678 | 6.4:-44134288 | MS.gene73327:CDS |
| TTCTTCAACGGATCACATTT+CGG | 0.311143 | 6.4:+44134934 | None:intergenic |
| AGGTCGATGTTTGCCTCTTT+GGG | 0.314652 | 6.4:-44134680 | MS.gene73327:CDS |
| CCAGCCACTTCTCTAGCATT+TGG | 0.320989 | 6.4:+44134808 | None:intergenic |
| TGGTTCAGAATGAGGTGTTT+TGG | 0.325299 | 6.4:-44134783 | MS.gene73327:CDS |
| CGCGTTTAAGGCAATGTTCA+TGG | 0.335480 | 6.4:-44134836 | MS.gene73327:CDS |
| TCTTAGTAGTCGACTTGTTT+GGG | 0.336957 | 6.4:-44134287 | MS.gene73327:CDS |
| CATGTGGATTTAAGAGTAAT+TGG | 0.347188 | 6.4:-44134195 | MS.gene73327:CDS |
| AATAATGATAGGAGTTTGTA+TGG | 0.354700 | 6.4:-44135396 | MS.gene73327:CDS |
| TGTGTGAGTTTAAATCTTTA+GGG | 0.363967 | 6.4:-44134255 | MS.gene73327:CDS |
| GTGTTGTGGTGGTGAGAGTT+TGG | 0.367651 | 6.4:-44135370 | MS.gene73327:CDS |
| AGAACAGGTGAATTCTGCTT+TGG | 0.381175 | 6.4:-44135676 | MS.gene73327:CDS |
| GAAGTACCAGGTGAAGTCTT+AGG | 0.382034 | 6.4:+44135708 | None:intergenic |
| ATCTTCGTCACTTCCAGAAA+TGG | 0.395777 | 6.4:+44134030 | None:intergenic |
| GATTATTAAAGGAATGGTTC+AGG | 0.407422 | 6.4:-44134728 | MS.gene73327:CDS |
| AGAATGCAGGAGATGGAGTA+TGG | 0.418553 | 6.4:-44134979 | MS.gene73327:CDS |
| AATACTTCTTGTCAGCTTCA+AGG | 0.424656 | 6.4:-44135129 | MS.gene73327:CDS |
| GTAGTAATTATAATAATGAT+AGG | 0.432117 | 6.4:-44135407 | MS.gene73327:CDS |
| TGAATCCGAGGCGAAAATTA+GGG | 0.434677 | 6.4:-44135484 | MS.gene73327:CDS |
| CAGCATCATTTGATGTTGTC+TGG | 0.438103 | 6.4:-44135792 | None:intergenic |
| AGGGTTTGTTAGTTTAATCA+AGG | 0.438901 | 6.4:-44135109 | MS.gene73327:CDS |
| TACTTCTTTGAGTAAAGAAC+AGG | 0.443784 | 6.4:-44135691 | MS.gene73327:CDS |
| TGCTGATAAGTTTAAGGCTA+AGG | 0.450816 | 6.4:-44135190 | MS.gene73327:CDS |
| CCGGTCCAAGGCTCGATGCT+AGG | 0.455130 | 6.4:-44135524 | MS.gene73327:CDS |
| ATCTCTTTGTTTCATCTGAA+CGG | 0.455464 | 6.4:+44134342 | None:intergenic |
| TTTATGGTGAATCTACCAAA+TGG | 0.459003 | 6.4:-44135342 | MS.gene73327:CDS |
| ATACTTCTTGTCAGCTTCAA+GGG | 0.488138 | 6.4:-44135128 | MS.gene73327:CDS |
| AGTGATGGTTGGAAGAGTTA+TGG | 0.491295 | 6.4:-44135438 | MS.gene73327:CDS |
| TGTGAAGATTATTAAAGGAA+TGG | 0.492323 | 6.4:-44134734 | MS.gene73327:CDS |
| AAGGCGGTGTTTACCGGCGG+GGG | 0.493111 | 6.4:-44135303 | MS.gene73327:CDS |
| ATTTGGAGAATCAAAACCAT+TGG | 0.493435 | 6.4:-44135158 | MS.gene73327:CDS |
| GTGTCTTTAATCAAGTAAAG+TGG | 0.493780 | 6.4:+44134526 | None:intergenic |
| TGCTAGAGAAGTGGCTGGAA+TGG | 0.497321 | 6.4:-44134803 | MS.gene73327:CDS |
| ACTTTCATCTTACCAGTGAC+TGG | 0.498140 | 6.4:+44134319 | None:intergenic |
| GACGCGAGGTTTCCGGTCCA+AGG | 0.501975 | 6.4:-44135536 | MS.gene73327:CDS |
| GAGACTGTGACTGGTGTTAG+TGG | 0.502637 | 6.4:-44135243 | MS.gene73327:CDS |
| ACTACACCACTCCTTCACTT+TGG | 0.506556 | 6.4:+44134645 | None:intergenic |
| GAGGCGGAGAGGCCGTTGAT+AGG | 0.507472 | 6.4:-44134700 | MS.gene73327:CDS |
| AGGAATGGTTCAGGATATAG+AGG | 0.509857 | 6.4:-44134719 | MS.gene73327:CDS |
| ACATTAAGATTGCATTGCAT+CGG | 0.512574 | 6.4:+44133985 | None:intergenic |
| TCTCAAGTTGGTTTGCCTGT+AGG | 0.521067 | 6.4:-44135570 | MS.gene73327:CDS |
| AGTTTGTATGGTTTGTGTTG+TGG | 0.522198 | 6.4:-44135384 | MS.gene73327:CDS |
| TGCAGGAGATGGAGTATGGC+GGG | 0.525001 | 6.4:-44134975 | MS.gene73327:CDS |
| ATCCTCAAGCATCGGAAACA+CGG | 0.525442 | 6.4:+44134903 | None:intergenic |
| CAAACAAGTCGACTACTAAG+AGG | 0.527797 | 6.4:+44134289 | None:intergenic |
| TTCAAGTAGCTAGTGATGGT+TGG | 0.529096 | 6.4:-44135449 | MS.gene73327:CDS |
| GTGTCAGAAACTTGAATGGC+GGG | 0.529427 | 6.4:+44134488 | None:intergenic |
| TAATTATAGAATGCAGGAGA+TGG | 0.530457 | 6.4:-44134986 | MS.gene73327:CDS |
| AAAAGATGATTTATGTGCAT+TGG | 0.530765 | 6.4:-44135766 | MS.gene73327:CDS |
| TTAAACCTAGCATCGAGCCT+TGG | 0.532416 | 6.4:+44135519 | None:intergenic |
| ATAAATGCCGCTGACCATGC+AGG | 0.535049 | 6.4:+44134556 | None:intergenic |
| TGAACGGCTTGCGCATAAAG+AGG | 0.540575 | 6.4:+44134358 | None:intergenic |
| TCGCGTGTCAGAAACTTGAA+TGG | 0.541251 | 6.4:+44134484 | None:intergenic |
| CGTCTCAAACAACTTCCTAC+AGG | 0.542256 | 6.4:+44135555 | None:intergenic |
| AAGAAGTATTTACCATCCAA+TGG | 0.543075 | 6.4:+44135142 | None:intergenic |
| GTCCGTGTTTCCGATGCTTG+AGG | 0.545204 | 6.4:-44134905 | MS.gene73327:CDS |
| TTATGGTGAATCTACCAAAT+GGG | 0.546125 | 6.4:-44135341 | MS.gene73327:CDS |
| TTGTATGGTTTGTGTTGTGG+TGG | 0.548848 | 6.4:-44135381 | MS.gene73327:CDS |
| CCTAGCATCGAGCCTTGGAC+CGG | 0.553355 | 6.4:+44135524 | None:intergenic |
| AACGGATCACATTTCGGCGA+AGG | 0.553597 | 6.4:+44134940 | None:intergenic |
| CTAATGAAATGGAGATCAGA+AGG | 0.554981 | 6.4:-44134388 | MS.gene73327:CDS |
| TTTCTTCATGCAACTTCATG+TGG | 0.555413 | 6.4:-44134211 | MS.gene73327:CDS |
| AAAAGGCGGTGTTTACCGGC+GGG | 0.560565 | 6.4:-44135305 | MS.gene73327:CDS |
| GGAGAATCAAAACCATTGGA+TGG | 0.563446 | 6.4:-44135154 | MS.gene73327:CDS |
| AAACAAAGAGATCCAGTCAC+TGG | 0.567086 | 6.4:-44134331 | MS.gene73327:CDS |
| CCAAATGCTAGAGAAGTGGC+TGG | 0.568259 | 6.4:-44134808 | MS.gene73327:CDS |
| AGTTTAAGGCTAAGGCATTG+AGG | 0.568636 | 6.4:-44135182 | MS.gene73327:CDS |
| AGTGATTCAAATGGTTGTGA+TGG | 0.570710 | 6.4:-44134863 | MS.gene73327:CDS |
| TCTTTACTCAAAGAAGTACC+AGG | 0.573758 | 6.4:+44135696 | None:intergenic |
| AGTGTTGTTCAAAGATGTGT+AGG | 0.575113 | 6.4:-44135219 | MS.gene73327:CDS |
| GTTCTCATCTCCTCCCAAAG+AGG | 0.577317 | 6.4:+44134667 | None:intergenic |
| CTCAAAATATCCTCAAGCAT+CGG | 0.586264 | 6.4:+44134895 | None:intergenic |
| TCGATGTTTGCCTCTTTGGG+AGG | 0.587276 | 6.4:-44134677 | MS.gene73327:CDS |
| GGCTGGAATGGTTCAGAATG+AGG | 0.587957 | 6.4:-44134791 | MS.gene73327:CDS |
| TAAATGCCGCTGACCATGCA+GGG | 0.606286 | 6.4:+44134557 | None:intergenic |
| AGTAAATACAATGTCGTCGA+AGG | 0.607878 | 6.4:-44134625 | MS.gene73327:CDS |
| GGAGATGAGAACCAAAGTGA+AGG | 0.608611 | 6.4:-44134656 | MS.gene73327:CDS |
| GAATTCGAAGTATGCTGAGG+AGG | 0.608662 | 6.4:-44135274 | MS.gene73327:CDS |
| GAACGGCTTGCGCATAAAGA+GGG | 0.613633 | 6.4:+44134359 | None:intergenic |
| GTGTTGTTCAAAGATGTGTA+GGG | 0.614226 | 6.4:-44135218 | MS.gene73327:CDS |
| GATTCAAATGGTTGTGATGG+AGG | 0.614297 | 6.4:-44134860 | MS.gene73327:CDS |
| TGAGAACCAAAGTGAAGGAG+TGG | 0.616062 | 6.4:-44134651 | MS.gene73327:CDS |
| GAAAAGGCGGTGTTTACCGG+CGG | 0.617999 | 6.4:-44135306 | MS.gene73327:CDS |
| CAATGTCGTCGAAGGACCAG+TGG | 0.618270 | 6.4:-44134617 | MS.gene73327:CDS |
| GGCAAACATCGACCTATCAA+CGG | 0.623513 | 6.4:+44134688 | None:intergenic |
| AGGAAGTTGTTTGAGACGCG+AGG | 0.625523 | 6.4:-44135550 | MS.gene73327:CDS |
| GGATCCAAATGCTAGAGAAG+TGG | 0.625781 | 6.4:-44134812 | MS.gene73327:CDS |
| CTGTGCTAGAGTGATTCAAA+TGG | 0.626672 | 6.4:-44134872 | MS.gene73327:CDS |
| AAGAGTCCTAAGACTTCACC+TGG | 0.630400 | 6.4:-44135714 | MS.gene73327:CDS |
| TACTTGATTAAAGACACAAG+TGG | 0.632678 | 6.4:-44134520 | MS.gene73327:CDS |
| CATCATTTGATGTTGTCTGG+TGG | 0.636248 | 6.4:-44135789 | None:intergenic |
| AAAGGCGGTGTTTACCGGCG+GGG | 0.650088 | 6.4:-44135304 | MS.gene73327:CDS |
| TAAATCTTTAGGGAAGATAG+CGG | 0.650273 | 6.4:-44134245 | MS.gene73327:CDS |
| CGTGTCAGAAACTTGAATGG+CGG | 0.650834 | 6.4:+44134487 | None:intergenic |
| TGAAGTTAAGAGTGAATCCG+AGG | 0.651148 | 6.4:-44135496 | MS.gene73327:CDS |
| ACGGACGCAAAGTTCTTCAA+CGG | 0.658006 | 6.4:+44134922 | None:intergenic |
| CTACCACCCTGCATGGTCAG+CGG | 0.663349 | 6.4:-44134563 | MS.gene73327:CDS |
| TAGACAATGAATCTCAGGTG+AGG | 0.669241 | 6.4:-44135020 | MS.gene73327:CDS |
| ATGCAGGAGATGGAGTATGG+CGG | 0.678386 | 6.4:-44134976 | MS.gene73327:CDS |
| AATGGTTCAGGATATAGAGG+CGG | 0.695270 | 6.4:-44134716 | MS.gene73327:CDS |
| TTCAGGATATAGAGGCGGAG+AGG | 0.697045 | 6.4:-44134711 | MS.gene73327:CDS |
| GGTGAATTCGAAGTATGCTG+AGG | 0.699279 | 6.4:-44135277 | MS.gene73327:CDS |
| GTTTAAGGCAATGTTCATGG+AGG | 0.722672 | 6.4:-44134833 | MS.gene73327:CDS |
| CTTTAGGGAAGATAGCGGTG+AGG | 0.746883 | 6.4:-44134240 | MS.gene73327:CDS |
| ATGCCGCTGACCATGCAGGG+TGG | 0.746921 | 6.4:+44134560 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAGTAATTATAATAATGAT+AGG | - | chr6.4:44134361-44134380 | MS.gene73327:CDS | 15.0% |
| !!! | TTTTGTTAATTATAGAATGC+AGG | - | chr6.4:44134776-44134795 | MS.gene73327:CDS | 20.0% |
| ! | AAAAGATGATTTATGTGCAT+TGG | - | chr6.4:44134002-44134021 | MS.gene73327:CDS | 25.0% |
| ! | AATAATGATAGGAGTTTGTA+TGG | - | chr6.4:44134372-44134391 | MS.gene73327:CDS | 25.0% |
| ! | TGTGAAGATTATTAAAGGAA+TGG | - | chr6.4:44135034-44135053 | MS.gene73327:CDS | 25.0% |
| ! | TGTGTGAGTTTAAATCTTTA+GGG | - | chr6.4:44135513-44135532 | MS.gene73327:CDS | 25.0% |
| ! | TTGTGTGAGTTTAAATCTTT+AGG | - | chr6.4:44135512-44135531 | MS.gene73327:CDS | 25.0% |
| !! | TTTTATAGACAATGAATCTC+AGG | - | chr6.4:44134743-44134762 | MS.gene73327:CDS | 25.0% |
| ACTAGCTACTTGAAAAAACA+TGG | + | chr6.4:44134313-44134332 | None:intergenic | 30.0% | |
| ATTTGGAGAATCAAAACCAT+TGG | - | chr6.4:44134610-44134629 | MS.gene73327:CDS | 30.0% | |
| CATGTGGATTTAAGAGTAAT+TGG | - | chr6.4:44135573-44135592 | MS.gene73327:CDS | 30.0% | |
| GATTATTAAAGGAATGGTTC+AGG | - | chr6.4:44135040-44135059 | MS.gene73327:CDS | 30.0% | |
| GATTGTTGCTGATAAGTTTA+AGG | - | chr6.4:44134572-44134591 | MS.gene73327:CDS | 30.0% | |
| GTGTCTTTAATCAAGTAAAG+TGG | + | chr6.4:44135245-44135264 | None:intergenic | 30.0% | |
| TAATTATAGAATGCAGGAGA+TGG | - | chr6.4:44134782-44134801 | MS.gene73327:CDS | 30.0% | |
| TACTTCTTTGAGTAAAGAAC+AGG | - | chr6.4:44134077-44134096 | MS.gene73327:CDS | 30.0% | |
| TACTTGATTAAAGACACAAG+TGG | - | chr6.4:44135248-44135267 | MS.gene73327:CDS | 30.0% | |
| TCGCTTGTGAAGATTATTAA+AGG | - | chr6.4:44135029-44135048 | MS.gene73327:CDS | 30.0% | |
| TCTTTCATGAGAAAAGTTTC+TGG | - | chr6.4:44135596-44135615 | MS.gene73327:CDS | 30.0% | |
| TGAGAGTTTGGTTAAGTTTA+TGG | - | chr6.4:44134410-44134429 | MS.gene73327:CDS | 30.0% | |
| TGGAGAAAATACTCGAAAAA+CGG | - | chr6.4:44135171-44135190 | MS.gene73327:CDS | 30.0% | |
| TTATGGTGAATCTACCAAAT+GGG | - | chr6.4:44134427-44134446 | MS.gene73327:CDS | 30.0% | |
| TTTATGGTGAATCTACCAAA+TGG | - | chr6.4:44134426-44134445 | MS.gene73327:CDS | 30.0% | |
| ! | ATCTCTTTGTTTCATCTGAA+CGG | + | chr6.4:44135429-44135448 | None:intergenic | 30.0% |
| ! | TAAATCTTTAGGGAAGATAG+CGG | - | chr6.4:44135523-44135542 | MS.gene73327:CDS | 30.0% |
| ! | TTTTGATGGAGCTAATGAAA+TGG | - | chr6.4:44135369-44135388 | MS.gene73327:CDS | 30.0% |
| !! | AAGAAGTATTTACCATCCAA+TGG | + | chr6.4:44134629-44134648 | None:intergenic | 30.0% |
| !! | AGGGTTTGTTAGTTTAATCA+AGG | - | chr6.4:44134659-44134678 | MS.gene73327:CDS | 30.0% |
| !!! | GATTGGTTTTATGAATGTTG+TGG | - | chr6.4:44134126-44134145 | MS.gene73327:CDS | 30.0% |
| !!! | TTTTTTCAAGTAGCTAGTGA+TGG | - | chr6.4:44134315-44134334 | MS.gene73327:CDS | 30.0% |
| AATACTTCTTGTCAGCTTCA+AGG | - | chr6.4:44134639-44134658 | MS.gene73327:CDS | 35.0% | |
| AGTAAATACAATGTCGTCGA+AGG | - | chr6.4:44135143-44135162 | MS.gene73327:CDS | 35.0% | |
| AGTGATTCAAATGGTTGTGA+TGG | - | chr6.4:44134905-44134924 | MS.gene73327:CDS | 35.0% | |
| ATACTTCTTGTCAGCTTCAA+GGG | - | chr6.4:44134640-44134659 | MS.gene73327:CDS | 35.0% | |
| CTAATGAAATGGAGATCAGA+AGG | - | chr6.4:44135380-44135399 | MS.gene73327:CDS | 35.0% | |
| CTCAAAATATCCTCAAGCAT+CGG | + | chr6.4:44134876-44134895 | None:intergenic | 35.0% | |
| TCAAAAACACTACTCCCATT+TGG | + | chr6.4:44134444-44134463 | None:intergenic | 35.0% | |
| TTCTTCAACGGATCACATTT+CGG | + | chr6.4:44134837-44134856 | None:intergenic | 35.0% | |
| TTTCTTCATGCAACTTCATG+TGG | - | chr6.4:44135557-44135576 | MS.gene73327:CDS | 35.0% | |
| ! | AGTGTTGTTCAAAGATGTGT+AGG | - | chr6.4:44134549-44134568 | MS.gene73327:CDS | 35.0% |
| ! | AGTTTGTATGGTTTGTGTTG+TGG | - | chr6.4:44134384-44134403 | MS.gene73327:CDS | 35.0% |
| ! | CAAGCTTTTCTTTCTCAAGT+TGG | - | chr6.4:44134186-44134205 | MS.gene73327:CDS | 35.0% |
| ! | CTTTGGATTTACTTGCTGAT+TGG | - | chr6.4:44134109-44134128 | MS.gene73327:CDS | 35.0% |
| ! | GCAGAATTGTTTTCCATTTC+TGG | - | chr6.4:44135725-44135744 | MS.gene73327:CDS | 35.0% |
| ! | GTGTTGTTCAAAGATGTGTA+GGG | - | chr6.4:44134550-44134569 | MS.gene73327:CDS | 35.0% |
| ! | TGCTGATAAGTTTAAGGCTA+AGG | - | chr6.4:44134578-44134597 | MS.gene73327:CDS | 35.0% |
| !! | AAGAAGAGATTTTTCTAGCG+AGG | - | chr6.4:44135691-44135710 | MS.gene73327:CDS | 35.0% |
| !! | CAAAAACTCTAGAGTTGCAT+TGG | - | chr6.4:44135622-44135641 | MS.gene73327:CDS | 35.0% |
| !! | GAGTAGTGTTTTTGAAAAGG+CGG | - | chr6.4:44134449-44134468 | MS.gene73327:CDS | 35.0% |
| !! | TCTTAGTAGTCGACTTGTTT+GGG | - | chr6.4:44135481-44135500 | MS.gene73327:CDS | 35.0% |
| !! | TCTTTACTCAAAGAAGTACC+AGG | + | chr6.4:44134075-44134094 | None:intergenic | 35.0% |
| !! | TGGGAGTAGTGTTTTTGAAA+AGG | - | chr6.4:44134446-44134465 | MS.gene73327:CDS | 35.0% |
| !!! | TTTTCGAGTATTTTCTCCAC+TGG | + | chr6.4:44135170-44135189 | None:intergenic | 35.0% |
| AAACAAAGAGATCCAGTCAC+TGG | - | chr6.4:44135437-44135456 | MS.gene73327:CDS | 40.0% | |
| AATGGTTCAGGATATAGAGG+CGG | - | chr6.4:44135052-44135071 | MS.gene73327:CDS | 40.0% | |
| ACTTTCATCTTACCAGTGAC+TGG | + | chr6.4:44135452-44135471 | None:intergenic | 40.0% | |
| AGAACAGGTGAATTCTGCTT+TGG | - | chr6.4:44134092-44134111 | MS.gene73327:CDS | 40.0% | |
| AGGAATGGTTCAGGATATAG+AGG | - | chr6.4:44135049-44135068 | MS.gene73327:CDS | 40.0% | |
| AGTGATGGTTGGAAGAGTTA+TGG | - | chr6.4:44134330-44134349 | MS.gene73327:CDS | 40.0% | |
| ATCTTCGTCACTTCCAGAAA+TGG | + | chr6.4:44135741-44135760 | None:intergenic | 40.0% | |
| CAAACAAGTCGACTACTAAG+AGG | + | chr6.4:44135482-44135501 | None:intergenic | 40.0% | |
| CTGTGCTAGAGTGATTCAAA+TGG | - | chr6.4:44134896-44134915 | MS.gene73327:CDS | 40.0% | |
| GATTCAAATGGTTGTGATGG+AGG | - | chr6.4:44134908-44134927 | MS.gene73327:CDS | 40.0% | |
| GGAGAATCAAAACCATTGGA+TGG | - | chr6.4:44134614-44134633 | MS.gene73327:CDS | 40.0% | |
| TAGACAATGAATCTCAGGTG+AGG | - | chr6.4:44134748-44134767 | MS.gene73327:CDS | 40.0% | |
| TGAAGTTAAGAGTGAATCCG+AGG | - | chr6.4:44134272-44134291 | MS.gene73327:CDS | 40.0% | |
| TGAATCCGAGGCGAAAATTA+GGG | - | chr6.4:44134284-44134303 | MS.gene73327:CDS | 40.0% | |
| TTCAAGTAGCTAGTGATGGT+TGG | - | chr6.4:44134319-44134338 | MS.gene73327:CDS | 40.0% | |
| TTTGAAAAGGCGGTGTTTAC+CGG | - | chr6.4:44134459-44134478 | MS.gene73327:CDS | 40.0% | |
| ! | AACAACGAGCTCGAACTTTT+CGG | - | chr6.4:44134687-44134706 | MS.gene73327:CDS | 40.0% |
| ! | GTTTAAGGCAATGTTCATGG+AGG | - | chr6.4:44134935-44134954 | MS.gene73327:CDS | 40.0% |
| ! | TTGTATGGTTTGTGTTGTGG+TGG | - | chr6.4:44134387-44134406 | MS.gene73327:CDS | 40.0% |
| !! | AGTTTAAGGCTAAGGCATTG+AGG | - | chr6.4:44134586-44134605 | MS.gene73327:CDS | 40.0% |
| !! | CTCTTAGTAGTCGACTTGTT+TGG | - | chr6.4:44135480-44135499 | MS.gene73327:CDS | 40.0% |
| !! | TGGTTCAGAATGAGGTGTTT+TGG | - | chr6.4:44134985-44135004 | MS.gene73327:CDS | 40.0% |
| !!! | AGAAGCTCATGTCGTTTTGA+TGG | - | chr6.4:44135355-44135374 | MS.gene73327:CDS | 40.0% |
| AAGAGTCCTAAGACTTCACC+TGG | - | chr6.4:44134054-44134073 | MS.gene73327:CDS | 45.0% | |
| ACGGACGCAAAGTTCTTCAA+CGG | + | chr6.4:44134849-44134868 | None:intergenic | 45.0% | |
| ACTACACCACTCCTTCACTT+TGG | + | chr6.4:44135126-44135145 | None:intergenic | 45.0% | |
| AGAATGCAGGAGATGGAGTA+TGG | - | chr6.4:44134789-44134808 | MS.gene73327:CDS | 45.0% | |
| AGGTCGATGTTTGCCTCTTT+GGG | - | chr6.4:44135088-44135107 | MS.gene73327:CDS | 45.0% | |
| CGTCTCAAACAACTTCCTAC+AGG | + | chr6.4:44134216-44134235 | None:intergenic | 45.0% | |
| CGTGTCAGAAACTTGAATGG+CGG | + | chr6.4:44135284-44135303 | None:intergenic | 45.0% | |
| GAAAAAACTACCACCCTGCA+TGG | - | chr6.4:44135198-44135217 | MS.gene73327:CDS | 45.0% | |
| GAATTCGAAGTATGCTGAGG+AGG | - | chr6.4:44134494-44134513 | MS.gene73327:CDS | 45.0% | |
| GGAGATGAGAACCAAAGTGA+AGG | - | chr6.4:44135112-44135131 | MS.gene73327:CDS | 45.0% | |
| GGATCCAAATGCTAGAGAAG+TGG | - | chr6.4:44134956-44134975 | MS.gene73327:CDS | 45.0% | |
| GGCAAACATCGACCTATCAA+CGG | + | chr6.4:44135083-44135102 | None:intergenic | 45.0% | |
| GGTGAATTCGAAGTATGCTG+AGG | - | chr6.4:44134491-44134510 | MS.gene73327:CDS | 45.0% | |
| GTGAATCCGAGGCGAAAATT+AGG | - | chr6.4:44134283-44134302 | MS.gene73327:CDS | 45.0% | |
| GTGTCAGAAACTTGAATGGC+GGG | + | chr6.4:44135283-44135302 | None:intergenic | 45.0% | |
| TAGGTCGATGTTTGCCTCTT+TGG | - | chr6.4:44135087-44135106 | MS.gene73327:CDS | 45.0% | |
| TCGCGTGTCAGAAACTTGAA+TGG | + | chr6.4:44135287-44135306 | None:intergenic | 45.0% | |
| TGAGAACCAAAGTGAAGGAG+TGG | - | chr6.4:44135117-44135136 | MS.gene73327:CDS | 45.0% | |
| ! | AGTATGCTGAGGAGGTTTTG+TGG | - | chr6.4:44134502-44134521 | MS.gene73327:CDS | 45.0% |
| ! | CGCGTTTAAGGCAATGTTCA+TGG | - | chr6.4:44134932-44134951 | MS.gene73327:CDS | 45.0% |
| ! | GAAGTACCAGGTGAAGTCTT+AGG | + | chr6.4:44134063-44134082 | None:intergenic | 45.0% |
| ! | TTAAACCTAGCATCGAGCCT+TGG | + | chr6.4:44134252-44134271 | None:intergenic | 45.0% |
| !! | ATCCTCAAGCATCGGAAACA+CGG | + | chr6.4:44134868-44134887 | None:intergenic | 45.0% |
| !! | GGCTAAGGCATTGAGGAATT+TGG | - | chr6.4:44134593-44134612 | MS.gene73327:CDS | 45.0% |
| !! | GTATGCTGAGGAGGTTTTGT+GGG | - | chr6.4:44134503-44134522 | MS.gene73327:CDS | 45.0% |
| !! | TCTCAAGTTGGTTTGCCTGT+AGG | - | chr6.4:44134198-44134217 | MS.gene73327:CDS | 45.0% |
| !!! | GAATGAGGTGTTTTGGAACG+AGG | - | chr6.4:44134992-44135011 | MS.gene73327:CDS | 45.0% |
| !!! | GTTTTGGAACGAGGTTGAAG+CGG | - | chr6.4:44135001-44135020 | MS.gene73327:CDS | 45.0% |
| AACGGATCACATTTCGGCGA+AGG | + | chr6.4:44134831-44134850 | None:intergenic | 50.0% | |
| ATAAATGCCGCTGACCATGC+AGG | + | chr6.4:44135215-44135234 | None:intergenic | 50.0% | |
| ATGCAGGAGATGGAGTATGG+CGG | - | chr6.4:44134792-44134811 | MS.gene73327:CDS | 50.0% | |
| CCAAATGCTAGAGAAGTGGC+TGG | - | chr6.4:44134960-44134979 | MS.gene73327:CDS | 50.0% | |
| CCAGCCACTTCTCTAGCATT+TGG | + | chr6.4:44134963-44134982 | None:intergenic | 50.0% | |
| GAACGGCTTGCGCATAAAGA+GGG | + | chr6.4:44135412-44135431 | None:intergenic | 50.0% | |
| GGCTGGAATGGTTCAGAATG+AGG | - | chr6.4:44134977-44134996 | MS.gene73327:CDS | 50.0% | |
| GTTCTCATCTCCTCCCAAAG+AGG | + | chr6.4:44135104-44135123 | None:intergenic | 50.0% | |
| TAAATGCCGCTGACCATGCA+GGG | + | chr6.4:44135214-44135233 | None:intergenic | 50.0% | |
| TCGATGTTTGCCTCTTTGGG+AGG | - | chr6.4:44135091-44135110 | MS.gene73327:CDS | 50.0% | |
| TGAACGGCTTGCGCATAAAG+AGG | + | chr6.4:44135413-44135432 | None:intergenic | 50.0% | |
| TGCTAGAGAAGTGGCTGGAA+TGG | - | chr6.4:44134965-44134984 | MS.gene73327:CDS | 50.0% | |
| TTCAGGATATAGAGGCGGAG+AGG | - | chr6.4:44135057-44135076 | MS.gene73327:CDS | 50.0% | |
| ! | AGGAAGTTGTTTGAGACGCG+AGG | - | chr6.4:44134218-44134237 | MS.gene73327:CDS | 50.0% |
| ! | CTTTAGGGAAGATAGCGGTG+AGG | - | chr6.4:44135528-44135547 | MS.gene73327:CDS | 50.0% |
| ! | GAGACTGTGACTGGTGTTAG+TGG | - | chr6.4:44134525-44134544 | MS.gene73327:CDS | 50.0% |
| ! | GGCATCCCTAATTTTCGCCT+CGG | + | chr6.4:44134292-44134311 | None:intergenic | 50.0% |
| ! | GTGTTGTGGTGGTGAGAGTT+TGG | - | chr6.4:44134398-44134417 | MS.gene73327:CDS | 50.0% |
| ! | GTTTTGTGGGAGACTGTGAC+TGG | - | chr6.4:44134516-44134535 | MS.gene73327:CDS | 50.0% |
| ! | TGTGATGGAGGACGCGTTTA+AGG | - | chr6.4:44134920-44134939 | MS.gene73327:CDS | 50.0% |
| ! | TGTTTGAGACGCGAGGTTTC+CGG | - | chr6.4:44134225-44134244 | MS.gene73327:CDS | 50.0% |
| AAAAGGCGGTGTTTACCGGC+GGG | - | chr6.4:44134463-44134482 | MS.gene73327:CDS | 55.0% | |
| CAATGTCGTCGAAGGACCAG+TGG | - | chr6.4:44135151-44135170 | MS.gene73327:CDS | 55.0% | |
| GAAAAGGCGGTGTTTACCGG+CGG | - | chr6.4:44134462-44134481 | MS.gene73327:CDS | 55.0% | |
| GTCCGTGTTTCCGATGCTTG+AGG | - | chr6.4:44134863-44134882 | MS.gene73327:CDS | 55.0% | |
| TGCAGGAGATGGAGTATGGC+GGG | - | chr6.4:44134793-44134812 | MS.gene73327:CDS | 55.0% | |
| AAAGGCGGTGTTTACCGGCG+GGG | - | chr6.4:44134464-44134483 | MS.gene73327:CDS | 60.0% | |
| CTACCACCCTGCATGGTCAG+CGG | - | chr6.4:44135205-44135224 | MS.gene73327:CDS | 60.0% | |
| ! | CCTAGCATCGAGCCTTGGAC+CGG | + | chr6.4:44134247-44134266 | None:intergenic | 60.0% |
| AAGGCGGTGTTTACCGGCGG+GGG | - | chr6.4:44134465-44134484 | MS.gene73327:CDS | 65.0% | |
| ATGCCGCTGACCATGCAGGG+TGG | + | chr6.4:44135211-44135230 | None:intergenic | 65.0% | |
| CCGGTCCAAGGCTCGATGCT+AGG | - | chr6.4:44134244-44134263 | MS.gene73327:CDS | 65.0% | |
| CGAATTCACCACTCCCCCGC+CGG | + | chr6.4:44134481-44134500 | None:intergenic | 65.0% | |
| GACGCGAGGTTTCCGGTCCA+AGG | - | chr6.4:44134232-44134251 | MS.gene73327:CDS | 65.0% | |
| GAGGCGGAGAGGCCGTTGAT+AGG | - | chr6.4:44135068-44135087 | MS.gene73327:CDS | 65.0% | |
| GGTGTTTACCGGCGGGGGAG+TGG | - | chr6.4:44134470-44134489 | MS.gene73327:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 44133988 | 44135802 | 44133988 | ID=MS.gene73327 |
| chr6.4 | mRNA | 44133988 | 44135802 | 44133988 | ID=MS.gene73327.t1;Parent=MS.gene73327 |
| chr6.4 | exon | 44133988 | 44135802 | 44133988 | ID=MS.gene73327.t1.exon1;Parent=MS.gene73327.t1 |
| chr6.4 | CDS | 44133988 | 44135802 | 44133988 | ID=cds.MS.gene73327.t1;Parent=MS.gene73327.t1 |
| Gene Sequence |
| Protein sequence |
