AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84426


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84426.t1 MTR_7g055747 95.938 320 13 0 27 346 110 429 0.0 540
MS.gene84426.t1 MTR_7g055560 95.938 320 13 0 27 346 110 429 0.0 540
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84426.t1 AT3G25130 45.902 183 73 5 182 343 228 405 3.37e-35 132

Find 56 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATATCTTCAATTGATTT+TGG 0.170861 7.4:-49048678 None:intergenic
CAAAGACTAAATGAAGATTT+TGG 0.193613 7.4:+49049062 MS.gene84426:CDS
AAATGAGTTGGAAGCATATT+TGG 0.211168 7.4:+49048620 MS.gene84426:CDS
TCAACAGGGAAGATGAATTT+AGG 0.246896 7.4:+49049416 MS.gene84426:CDS
TGTTCTAATCTTGGAAGTTT+TGG 0.255756 7.4:+49049143 MS.gene84426:CDS
ACACTATGGTCCATTGAAAA+TGG 0.271896 7.4:+49048984 MS.gene84426:CDS
GTTTAGTGAGGATGAGTTAG+AGG 0.283368 7.4:+49049346 MS.gene84426:CDS
GATGATAAAGATGAGGAAAT+TGG 0.284728 7.4:+49048591 MS.gene84426:CDS
TCCAATACCTTTCAAAGCTT+TGG 0.297202 7.4:-49049468 None:intergenic
TCCAAAGCTTTGAAAGGTAT+TGG 0.299212 7.4:+49049467 MS.gene84426:CDS
GAAGAAGTTTGTTCTAATCT+TGG 0.301550 7.4:+49049134 MS.gene84426:CDS
AAAATATGGAGAAACATAAA+TGG 0.320936 7.4:-49048320 None:intergenic
AGAAGTAGTCAAGCCTATAT+TGG 0.332436 7.4:+49048926 MS.gene84426:CDS
TGAGGATGAGTTAGAGGAAG+AGG 0.350921 7.4:+49049352 MS.gene84426:CDS
TTGAATTTAGTATCCAATAT+AGG 0.358936 7.4:-49048939 None:intergenic
CAAGCTTTGAAATTCTCAAC+AGG 0.378595 7.4:+49049401 MS.gene84426:CDS
GTGAAGGAATGGATATGCTT+TGG 0.388433 7.4:+49049246 MS.gene84426:CDS
AGAGAACTCATTGTTCTTGT+TGG 0.389427 7.4:-49048274 None:intergenic
GGACGTAGTTGAATCCATTA+AGG 0.402615 7.4:+49048815 MS.gene84426:CDS
GAGTATGGTGGAAAAGGTAT+AGG 0.411829 7.4:+49049104 MS.gene84426:CDS
AAAAGAGGTTTATTGTCAAA+TGG 0.412280 7.4:+49048743 MS.gene84426:CDS
AGTATGGTGGAAAAGGTATA+GGG 0.423952 7.4:+49049105 MS.gene84426:CDS
TTGGAGAATTTGACAAGGTT+AGG 0.431973 7.4:-49049449 None:intergenic
TGAAGGAATGGATATGCTTT+GGG 0.440242 7.4:+49049247 MS.gene84426:CDS
AAGAAAAGTAACACAAAGAA+AGG 0.440269 7.4:+49049302 MS.gene84426:CDS
AATTGGTTTGCTAAATGAGT+TGG 0.447975 7.4:+49048608 MS.gene84426:CDS
AGAAAATGTGTCTTGTGAAA+AGG 0.452614 7.4:+49048896 MS.gene84426:CDS
GTCTCTATTACCCTTCACTT+TGG 0.467195 7.4:-49049009 None:intergenic
GAGTTAGAGGAAGAGGAGGT+AGG 0.471390 7.4:+49049359 MS.gene84426:CDS
TTGCATCATGTAGGCAAGAA+TGG 0.480234 7.4:+49049494 MS.gene84426:CDS
AATTGGGAGTATGGTGGAAA+AGG 0.481166 7.4:+49049098 MS.gene84426:CDS
GAGAGACATAATAATAGTGA+TGG 0.502040 7.4:+49049221 MS.gene84426:CDS
AGAAAACAAAGTTGAGAAAG+TGG 0.508967 7.4:+49048794 MS.gene84426:CDS
GGATACTAAATTCAATAAAG+TGG 0.514253 7.4:+49048947 MS.gene84426:CDS
AGGGAAGATGAATTTAGGAA+TGG 0.516981 7.4:+49049421 MS.gene84426:CDS
GAACTTCAAAGATTGAAGCT+TGG 0.517540 7.4:-49048650 None:intergenic
AAGCTTTGAAAGGTATTGGA+TGG 0.532498 7.4:+49049471 MS.gene84426:CDS
TAGTGATGGAAGTGAAGGAA+TGG 0.536155 7.4:+49049235 MS.gene84426:CDS
GGGAAGATGAATTTAGGAAT+GGG 0.537973 7.4:+49049422 MS.gene84426:CDS
GGATGAGTTAGAGGAAGAGG+AGG 0.538629 7.4:+49049355 MS.gene84426:CDS
AAGTGAAGTTGAGTTTAGTG+AGG 0.546215 7.4:+49049334 MS.gene84426:CDS
AATAATAGTGATGGAAGTGA+AGG 0.548220 7.4:+49049230 MS.gene84426:CDS
AAGAAAGCAAAGAAACACTA+TGG 0.554986 7.4:+49048970 MS.gene84426:CDS
AGAAAAGTAACACAAAGAAA+GGG 0.575353 7.4:+49049303 MS.gene84426:CDS
AAATTCTCCAAAGCTTTGAA+AGG 0.585068 7.4:+49049461 MS.gene84426:CDS
TGAAAATGGATCCAAAGTGA+AGG 0.588660 7.4:+49048998 MS.gene84426:CDS
AAAAGAGTGGAGAAGGACAT+TGG 0.588797 7.4:+49049181 MS.gene84426:CDS
GAAAATGGATCCAAAGTGAA+GGG 0.600047 7.4:+49048999 MS.gene84426:CDS
AAAAGCAATTGGGAGTATGG+TGG 0.613321 7.4:+49049092 MS.gene84426:CDS
TGAAGCTGATGATAAAGATG+AGG 0.620705 7.4:+49048584 MS.gene84426:CDS
ATTGGATGGTTGCATCATGT+AGG 0.636955 7.4:+49049485 MS.gene84426:CDS
AGATGAATTTAGGAATGGGA+AGG 0.640076 7.4:+49049426 MS.gene84426:CDS
AAGCTTTGAAATTCTCAACA+GGG 0.664595 7.4:+49049402 MS.gene84426:CDS
AATGGAACAAGACAGTGAAG+AGG 0.664688 7.4:-49048302 None:intergenic
AAGCTTTGGAGAATTTGACA+AGG 0.677569 7.4:-49049454 None:intergenic
ATATGAGAAAGAATCAAACA+AGG 0.721458 7.4:+49049274 MS.gene84426:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATAGGAAAAATATAA+AGG - chr7.4:49048351-49048370 None:intergenic 15.0%
!! GAAAATAGGAAAAATATAAA+GGG - chr7.4:49048350-49048369 None:intergenic 15.0%
!! AAAATATGGAGAAACATAAA+TGG - chr7.4:49048323-49048342 None:intergenic 20.0%
!! TTGAATTTAGTATCCAATAT+AGG - chr7.4:49048942-49048961 None:intergenic 20.0%
!!! GAAATATCTTCAATTGATTT+TGG - chr7.4:49048681-49048700 None:intergenic 20.0%
! AAAAGAGGTTTATTGTCAAA+TGG + chr7.4:49048743-49048762 MS.gene84426:CDS 25.0%
! AAAGAAAAGATAGGAGTTTA+AGG - chr7.4:49048392-49048411 None:intergenic 25.0%
! AAGAAAAGTAACACAAAGAA+AGG + chr7.4:49049302-49049321 MS.gene84426:CDS 25.0%
! AGAAAAGTAACACAAAGAAA+GGG + chr7.4:49049303-49049322 MS.gene84426:CDS 25.0%
! AGAAAGAAAAAAACACCATT+TGG - chr7.4:49048538-49048557 None:intergenic 25.0%
! ATATAAAGGGTGAGAAAATA+TGG - chr7.4:49048337-49048356 None:intergenic 25.0%
! ATATGAGAAAGAATCAAACA+AGG + chr7.4:49049274-49049293 MS.gene84426:CDS 25.0%
! GGATACTAAATTCAATAAAG+TGG + chr7.4:49048947-49048966 MS.gene84426:CDS 25.0%
!! CAAAGACTAAATGAAGATTT+TGG + chr7.4:49049062-49049081 MS.gene84426:CDS 25.0%
!!! TAACACTTTTTCTTCCTTAA+TGG - chr7.4:49048832-49048851 None:intergenic 25.0%
AAGAAAGCAAAGAAACACTA+TGG + chr7.4:49048970-49048989 MS.gene84426:CDS 30.0%
ACAAAGGAGAAAGAAAAGAT+AGG - chr7.4:49048401-49048420 None:intergenic 30.0%
AGAAAACAAAGTTGAGAAAG+TGG + chr7.4:49048794-49048813 MS.gene84426:CDS 30.0%
AGAAAATGTGTCTTGTGAAA+AGG + chr7.4:49048896-49048915 MS.gene84426:CDS 30.0%
GAGAGACATAATAATAGTGA+TGG + chr7.4:49049221-49049240 MS.gene84426:CDS 30.0%
GATAGGAGTTTAAGGATATA+TGG - chr7.4:49048384-49048403 None:intergenic 30.0%
GATGATAAAGATGAGGAAAT+TGG + chr7.4:49048591-49048610 MS.gene84426:CDS 30.0%
TGAAAGTAAGAAAAGCAACA+AGG - chr7.4:49048446-49048465 None:intergenic 30.0%
TGAAGAGAAAAAAGTGAACT+TGG + chr7.4:49048767-49048786 MS.gene84426:CDS 30.0%
TGGAGAAAAGAAGAGAAAAT+AGG - chr7.4:49048364-49048383 None:intergenic 30.0%
! AAATGAGTTGGAAGCATATT+TGG + chr7.4:49048620-49048639 MS.gene84426:CDS 30.0%
! AATAATAGTGATGGAAGTGA+AGG + chr7.4:49049230-49049249 MS.gene84426:CDS 30.0%
! GAAGAAGTTTGTTCTAATCT+TGG + chr7.4:49049134-49049153 MS.gene84426:CDS 30.0%
! GTTTTCAGTTGAAGAAAAAG+AGG + chr7.4:49048728-49048747 MS.gene84426:CDS 30.0%
! TGTTCTAATCTTGGAAGTTT+TGG + chr7.4:49049143-49049162 MS.gene84426:CDS 30.0%
!! AAATTCTCCAAAGCTTTGAA+AGG + chr7.4:49049461-49049480 MS.gene84426:CDS 30.0%
!! AAGCTTTGAAATTCTCAACA+GGG + chr7.4:49049402-49049421 MS.gene84426:CDS 30.0%
!! AATTGGTTTGCTAAATGAGT+TGG + chr7.4:49048608-49048627 MS.gene84426:CDS 30.0%
!! TTTTGCAAACTTTTCTAGCA+TGG + chr7.4:49048559-49048578 MS.gene84426:intron 30.0%
AAGTGAAGTTGAGTTTAGTG+AGG + chr7.4:49049334-49049353 MS.gene84426:CDS 35.0%
ACACTATGGTCCATTGAAAA+TGG + chr7.4:49048984-49049003 MS.gene84426:CDS 35.0%
AGAAGTAGTCAAGCCTATAT+TGG + chr7.4:49048926-49048945 MS.gene84426:CDS 35.0%
AGAGAACTCATTGTTCTTGT+TGG - chr7.4:49048277-49048296 None:intergenic 35.0%
AGATGAATTTAGGAATGGGA+AGG + chr7.4:49049426-49049445 MS.gene84426:CDS 35.0%
AGGGAAGATGAATTTAGGAA+TGG + chr7.4:49049421-49049440 MS.gene84426:CDS 35.0%
CCAAACCTTGTTCATCATAA+GGG + chr7.4:49048468-49048487 MS.gene84426:intron 35.0%
GAAAATGGATCCAAAGTGAA+GGG + chr7.4:49048999-49049018 MS.gene84426:CDS 35.0%
GAACTTCAAAGATTGAAGCT+TGG - chr7.4:49048653-49048672 None:intergenic 35.0%
GGGAAGATGAATTTAGGAAT+GGG + chr7.4:49049422-49049441 MS.gene84426:CDS 35.0%
TACTACCCTTATGATGAACA+AGG - chr7.4:49048476-49048495 None:intergenic 35.0%
TCAACAGGGAAGATGAATTT+AGG + chr7.4:49049416-49049435 MS.gene84426:CDS 35.0%
TCCAAACCTTGTTCATCATA+AGG + chr7.4:49048467-49048486 MS.gene84426:intron 35.0%
TCCAATACCTTTCAAAGCTT+TGG - chr7.4:49049471-49049490 None:intergenic 35.0%
TGAAAATGGATCCAAAGTGA+AGG + chr7.4:49048998-49049017 MS.gene84426:CDS 35.0%
TGAAGCTGATGATAAAGATG+AGG + chr7.4:49048584-49048603 MS.gene84426:CDS 35.0%
TGAAGGAATGGATATGCTTT+GGG + chr7.4:49049247-49049266 MS.gene84426:CDS 35.0%
TGGAAGTCCAAAAAGCAATT+GGG + chr7.4:49049082-49049101 MS.gene84426:CDS 35.0%
TTGAGAGTTATGAGTCCAAA+TGG + chr7.4:49048520-49048539 MS.gene84426:intron 35.0%
TTGGAAGTCCAAAAAGCAAT+TGG + chr7.4:49049081-49049100 MS.gene84426:CDS 35.0%
TTGGAGAATTTGACAAGGTT+AGG - chr7.4:49049452-49049471 None:intergenic 35.0%
! AGAGTAGTGGTAATGAACAA+AGG - chr7.4:49048417-49048436 None:intergenic 35.0%
! AGTATGGTGGAAAAGGTATA+GGG + chr7.4:49049105-49049124 MS.gene84426:CDS 35.0%
! CACTTTGGATCCATTTTCAA+TGG - chr7.4:49048997-49049016 None:intergenic 35.0%
!! AAGCTTTGAAAGGTATTGGA+TGG + chr7.4:49049471-49049490 MS.gene84426:CDS 35.0%
!! AAGCTTTGGAGAATTTGACA+AGG - chr7.4:49049457-49049476 None:intergenic 35.0%
!! CAAGCTTTGAAATTCTCAAC+AGG + chr7.4:49049401-49049420 MS.gene84426:CDS 35.0%
!! TCCAAAGCTTTGAAAGGTAT+TGG + chr7.4:49049467-49049486 MS.gene84426:CDS 35.0%
!!! AAGTTTTGGTTCAATGAGAG+TGG + chr7.4:49049157-49049176 MS.gene84426:CDS 35.0%
AAAAGAGTGGAGAAGGACAT+TGG + chr7.4:49049181-49049200 MS.gene84426:CDS 40.0%
AACAAGGAGAAGAAGAGTAG+TGG - chr7.4:49048430-49048449 None:intergenic 40.0%
AATGGAACAAGACAGTGAAG+AGG - chr7.4:49048305-49048324 None:intergenic 40.0%
ATTGGATGGTTGCATCATGT+AGG + chr7.4:49049485-49049504 MS.gene84426:CDS 40.0%
CAATGAGAGTGGAAAAAGAG+TGG + chr7.4:49049168-49049187 MS.gene84426:CDS 40.0%
CCAAAAAGCAATTGGGAGTA+TGG + chr7.4:49049089-49049108 MS.gene84426:CDS 40.0%
CCCTTATGATGAACAAGGTT+TGG - chr7.4:49048471-49048490 None:intergenic 40.0%
GGACGTAGTTGAATCCATTA+AGG + chr7.4:49048815-49048834 MS.gene84426:CDS 40.0%
GTCTCTATTACCCTTCACTT+TGG - chr7.4:49049012-49049031 None:intergenic 40.0%
GTGAAGGAATGGATATGCTT+TGG + chr7.4:49049246-49049265 MS.gene84426:CDS 40.0%
GTTTAGTGAGGATGAGTTAG+AGG + chr7.4:49049346-49049365 MS.gene84426:CDS 40.0%
TTGCATCATGTAGGCAAGAA+TGG + chr7.4:49049494-49049513 MS.gene84426:CDS 40.0%
! AAAAGCAATTGGGAGTATGG+TGG + chr7.4:49049092-49049111 MS.gene84426:CDS 40.0%
! AATTGGGAGTATGGTGGAAA+AGG + chr7.4:49049098-49049117 MS.gene84426:CDS 40.0%
! GAGTATGGTGGAAAAGGTAT+AGG + chr7.4:49049104-49049123 MS.gene84426:CDS 40.0%
! TAGTGATGGAAGTGAAGGAA+TGG + chr7.4:49049235-49049254 MS.gene84426:CDS 40.0%
!! CCATACTCCCAATTGCTTTT+TGG - chr7.4:49049092-49049111 None:intergenic 40.0%
!!! ATTGGCTTGCAAGCTTTTTG+AGG + chr7.4:49049199-49049218 MS.gene84426:CDS 40.0%
GAGTGGAAAAAGAGTGGAGA+AGG + chr7.4:49049174-49049193 MS.gene84426:CDS 45.0%
TGAGGATGAGTTAGAGGAAG+AGG + chr7.4:49049352-49049371 MS.gene84426:CDS 45.0%
GAGTTAGAGGAAGAGGAGGT+AGG + chr7.4:49049359-49049378 MS.gene84426:CDS 50.0%
GGATGAGTTAGAGGAAGAGG+AGG + chr7.4:49049355-49049374 MS.gene84426:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 49048246 49049535 49048246 ID=MS.gene84426
chr7.4 mRNA 49048246 49049535 49048246 ID=MS.gene84426.t1;Parent=MS.gene84426
chr7.4 exon 49048246 49048327 49048246 ID=MS.gene84426.t1.exon1;Parent=MS.gene84426.t1
chr7.4 CDS 49048246 49048327 49048246 ID=cds.MS.gene84426.t1;Parent=MS.gene84426.t1
chr7.4 exon 49048577 49049535 49048577 ID=MS.gene84426.t1.exon2;Parent=MS.gene84426.t1
chr7.4 CDS 49048577 49049535 49048577 ID=cds.MS.gene84426.t1;Parent=MS.gene84426.t1
Gene Sequence

>MS.gene84426

ATGAGTGAGTTATCTTTCTCAAATGCTTCCAACAAGAACAATGAGTTCTCTAGTAACCTCTTCACTGTCTTGTTCCATTTATGTTTCTCCATATTTTCTCACCCTTTATATTTTTCCTATTTTCTCTTCTTTTCTCCATATATCCTTAAACTCCTATCTTTTCTTTCTCCTTTGTTCATTACCACTACTCTTCTTCTCCTTGTTGCTTTTCTTACTTTCACTCCAAACCTTGTTCATCATAAGGGTAGTAGCAAAAGTACTAGTACTAGTAGTGTTGAGAGTTATGAGTCCAAATGGTGTTTTTTTCTTTCTATTTTGCAAACTTTTCTAGCATGGTTTGAAGCTGATGATAAAGATGAGGAAATTGGTTTGCTAAATGAGTTGGAAGCATATTTGGTTATGTTCCAAGCTTCAATCTTTGAAGTTCATGAGCCAAAATCAATTGAAGATATTTCTGAAGAGTTTGAAGAAGCAGATGACGAGTTTTCAGTTGAAGAAAAAGAGGTTTATTGTCAAATGGATGAAGAGAAAAAAGTGAACTTGGATGAAGAAAACAAAGTTGAGAAAGTGGACGTAGTTGAATCCATTAAGGAAGAAAAAGTGTTAGATGTGAAAAGTTTAGTAACTTTGTTTCAAGAATATGCAGAGTTAGAAAATGTGTCTTGTGAAAAGGAAGAAAAAGAAGTAGTCAAGCCTATATTGGATACTAAATTCAATAAAGTGGAAGAAAGCAAAGAAACACTATGGTCCATTGAAAATGGATCCAAAGTGAAGGGTAATAGAGACATGTATGCAAATAAAGTGAAAGTGAAATCTCAAAGACTAAATGAAGATTTTGGAAGTCCAAAAAGCAATTGGGAGTATGGTGGAAAAGGTATAGGGAATAATGAAGAAGTTTGTTCTAATCTTGGAAGTTTTGGTTCAATGAGAGTGGAAAAAGAGTGGAGAAGGACATTGGCTTGCAAGCTTTTTGAGGAGAGACATAATAATAGTGATGGAAGTGAAGGAATGGATATGCTTTGGGAAACATATGAGAAAGAATCAAACAAGGTTGTGAAGAAAAGTAACACAAAGAAAGGGAAGAAATTAAGTGAAGTTGAGTTTAGTGAGGATGAGTTAGAGGAAGAGGAGGTAGGAGCTAAGTTATGTTGTTTACAAGCTTTGAAATTCTCAACAGGGAAGATGAATTTAGGAATGGGAAGGCCTAACCTTGTCAAATTCTCCAAAGCTTTGAAAGGTATTGGATGGTTGCATCATGTAGGCAAGAATGGAAAGAAGAATAACCACTGA

Protein sequence

>MS.gene84426.t1

MSELSFSNASNKNNEFSSNLFTVLFHLSWFEADDKDEEIGLLNELEAYLVMFQASIFEVHEPKSIEDISEEFEEADDEFSVEEKEVYCQMDEEKKVNLDEENKVEKVDVVESIKEEKVLDVKSLVTLFQEYAELENVSCEKEEKEVVKPILDTKFNKVEESKETLWSIENGSKVKGNRDMYANKVKVKSQRLNEDFGSPKSNWEYGGKGIGNNEEVCSNLGSFGSMRVEKEWRRTLACKLFEERHNNSDGSEGMDMLWETYEKESNKVVKKSNTKKGKKLSEVEFSEDELEEEEVGAKLCCLQALKFSTGKMNLGMGRPNLVKFSKALKGIGWLHHVGKNGKKNNH