AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064944


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064944.t1 MTR_1g046070 96.996 233 5 1 1 231 1 233 8.00e-155 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064944.t1 AT4G13540 46.061 165 71 4 49 212 37 184 2.25e-32 117
MS.gene064944.t1 AT3G23930 41.279 172 78 4 52 212 40 199 1.88e-23 94.4

Find 48 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTACCATCATATGTTCTT+TGG 0.233440 1.4:+30540501 None:intergenic
TGAGAAAGAGTGGGAATTAA+TGG 0.247721 1.4:-30540636 MS.gene064944:CDS
GAAGAAGTTGAAAATCAAAT+TGG 0.250274 1.4:-30539661 MS.gene064944:CDS
TTCTTCTCTTTAGTTTGAAT+TGG 0.254934 1.4:+30539681 None:intergenic
AAAGCTTTAAAAGCTCAATT+AGG 0.297525 1.4:-30539789 MS.gene064944:CDS
ATTAATGGGGACTAAGCTTT+TGG 0.315398 1.4:-30540621 MS.gene064944:CDS
AAAGGGAATTTGACTTGTTA+AGG 0.331176 1.4:-30539737 MS.gene064944:CDS
TAAAGGAGATAATGTGTGAT+AGG 0.355573 1.4:-30540835 MS.gene064944:CDS
ACTTCTACAAGAAGAAATAA+AGG 0.374088 1.4:-30540852 MS.gene064944:CDS
GAACTATGAGAGGGAGATAA+TGG 0.387809 1.4:-30540798 MS.gene064944:CDS
GAGAAAGAGTGGGAATTAAT+GGG 0.405188 1.4:-30540635 MS.gene064944:CDS
GAGGGAAGTGGTGGAAGAGA+AGG 0.429388 1.4:-30540708 MS.gene064944:CDS
AAGAGAAGGAAGAGAAGATA+AGG 0.430927 1.4:-30540694 MS.gene064944:CDS
AAGAAGTTGAAAATCAAATT+GGG 0.435875 1.4:-30539660 MS.gene064944:CDS
GGTGAAGCAGTTGAGGGAAG+TGG 0.438865 1.4:-30540720 MS.gene064944:CDS
AAGAAGAGGTGAAGCAGTTG+AGG 0.440787 1.4:-30540727 MS.gene064944:CDS
TCACAGGAGATGGTTTGTAT+TGG 0.451338 1.4:-30539881 MS.gene064944:intron
AGAAGAATTGTGAGAAAGAG+TGG 0.454742 1.4:-30540646 MS.gene064944:CDS
ATAAGGGAGATGGAAGAGGT+TGG 0.460472 1.4:-30540677 MS.gene064944:CDS
ACTTGTTAAGGCAAAGTTTG+AGG 0.469148 1.4:-30539725 MS.gene064944:CDS
AAGAAAGGTACAAACAAGAA+AGG 0.469395 1.4:-30539755 MS.gene064944:CDS
AGAGAAGGAAGAGAAGATAA+GGG 0.476340 1.4:-30540693 MS.gene064944:CDS
AGAAGAGGTGAAGCAGTTGA+GGG 0.480459 1.4:-30540726 MS.gene064944:CDS
AAGAGAGTAGGAACTATGAG+AGG 0.487797 1.4:-30540808 MS.gene064944:CDS
GAAGATAAGGGAGATGGAAG+AGG 0.488344 1.4:-30540681 MS.gene064944:CDS
TACAAATTGAAAATCTGAAG+AGG 0.492525 1.4:-30539839 MS.gene064944:CDS
GTGATAGGGAGAAAGAGAGT+AGG 0.501313 1.4:-30540820 MS.gene064944:CDS
GGAAGAGAAGATAAGGGAGA+TGG 0.503969 1.4:-30540687 MS.gene064944:CDS
GAGATGAAGCTGTTGAGAAG+TGG 0.508742 1.4:-30540568 MS.gene064944:CDS
TAGGTTCAGTGGAGAAAGAA+AGG 0.512086 1.4:-30539770 MS.gene064944:CDS
GAAGCAGTTGAGGGAAGTGG+TGG 0.513840 1.4:-30540717 MS.gene064944:CDS
AAAAGCTCAATTAGGTTCAG+TGG 0.518823 1.4:-30539781 MS.gene064944:CDS
AGAGAGTAGGAACTATGAGA+GGG 0.522297 1.4:-30540807 MS.gene064944:CDS
CTCATCCAAAGAACATATGA+TGG 0.522947 1.4:-30540506 MS.gene064944:intron
AAAGGAGATAATGTGTGATA+GGG 0.526845 1.4:-30540834 MS.gene064944:CDS
AGAAAGGTACAAACAAGAAA+GGG 0.530283 1.4:-30539754 MS.gene064944:CDS
GGAGATGGAAGAGGTTGGAA+TGG 0.546010 1.4:-30540672 MS.gene064944:CDS
CAAAGTTTGAGGATCATGAA+TGG 0.562620 1.4:-30539714 MS.gene064944:CDS
GGTGAAGAGGTTGAAAGAAG+AGG 0.569093 1.4:-30540741 MS.gene064944:CDS
ATTGGAAACAAGAGGTGAAG+AGG 0.596605 1.4:-30540754 MS.gene064944:CDS
ACAAATTGAAAATCTGAAGA+GGG 0.598118 1.4:-30539838 MS.gene064944:CDS
GAAGAATTGTGAGAAAGAGT+GGG 0.599083 1.4:-30540645 MS.gene064944:CDS
AGAAAGAGTGGGAATTAATG+GGG 0.618319 1.4:-30540634 MS.gene064944:CDS
AGATGAAGGAGGAAAGAGCA+AGG 0.618929 1.4:-30540592 MS.gene064944:CDS
TGGTTTGTATTGGAAAGCTG+AGG 0.636251 1.4:-30539871 MS.gene064944:CDS
GGAGGCAGATTGGAAACAAG+AGG 0.643296 1.4:-30540762 MS.gene064944:CDS
TTTGGTTCAAGAGATGAAGG+AGG 0.645416 1.4:-30540603 MS.gene064944:CDS
GGGGCCAATTCAAAGATGGG+GGG 0.731569 1.4:-30540950 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTCATTACATATTTTTG+TGG + chr1.4:30539701-30539720 None:intergenic 15.0%
!! AAGAAGTTGAAAATCAAATT+GGG - chr1.4:30540928-30540947 MS.gene064944:CDS 20.0%
!! ATTAATATGTGACAATAATG+TGG - chr1.4:30540588-30540607 MS.gene064944:CDS 20.0%
!!! AATTTTGAAAAGTACTATGA+TGG + chr1.4:30540563-30540582 None:intergenic 20.0%
!!! ATTTTGAAAAGTACTATGAT+GGG + chr1.4:30540562-30540581 None:intergenic 20.0%
! ACAAATTGAAAATCTGAAGA+GGG - chr1.4:30540750-30540769 MS.gene064944:CDS 25.0%
! ACTTCTACAAGAAGAAATAA+AGG - chr1.4:30539736-30539755 MS.gene064944:CDS 25.0%
! GAAGAAGTTGAAAATCAAAT+TGG - chr1.4:30540927-30540946 MS.gene064944:CDS 25.0%
! TACAAACAGTACTAATTGTT+TGG + chr1.4:30540461-30540480 None:intergenic 25.0%
! TACAAATTGAAAATCTGAAG+AGG - chr1.4:30540749-30540768 MS.gene064944:CDS 25.0%
!!! AAAGCTTTAAAAGCTCAATT+AGG - chr1.4:30540799-30540818 MS.gene064944:CDS 25.0%
!!! TTCTTCTCTTTAGTTTGAAT+TGG + chr1.4:30540910-30540929 None:intergenic 25.0%
AAAGGAGATAATGTGTGATA+GGG - chr1.4:30539754-30539773 MS.gene064944:CDS 30.0%
TAAAGGAGATAATGTGTGAT+AGG - chr1.4:30539753-30539772 MS.gene064944:CDS 30.0%
! AAGAAAGGTACAAACAAGAA+AGG - chr1.4:30540833-30540852 MS.gene064944:CDS 30.0%
! AGAAAGGTACAAACAAGAAA+GGG - chr1.4:30540834-30540853 MS.gene064944:CDS 30.0%
! TACTTGAGCTTATTTGCTTT+TGG - chr1.4:30540676-30540695 MS.gene064944:CDS 30.0%
!! AAAGGGAATTTGACTTGTTA+AGG - chr1.4:30540851-30540870 MS.gene064944:CDS 30.0%
!! GATAATGGTGTTTGCTTTTA+AGG - chr1.4:30539805-30539824 MS.gene064944:CDS 30.0%
AAAAGCTCAATTAGGTTCAG+TGG - chr1.4:30540807-30540826 MS.gene064944:CDS 35.0%
AAGAGAAGGAAGAGAAGATA+AGG - chr1.4:30539894-30539913 MS.gene064944:intron 35.0%
AGAAAGAGTGGGAATTAATG+GGG - chr1.4:30539954-30539973 MS.gene064944:intron 35.0%
AGAAGAATTGTGAGAAAGAG+TGG - chr1.4:30539942-30539961 MS.gene064944:intron 35.0%
AGAGAAGGAAGAGAAGATAA+GGG - chr1.4:30539895-30539914 MS.gene064944:intron 35.0%
CAAAGTTTGAGGATCATGAA+TGG - chr1.4:30540874-30540893 MS.gene064944:CDS 35.0%
CTCATCCAAAGAACATATGA+TGG - chr1.4:30540082-30540101 MS.gene064944:intron 35.0%
GAAGAATTGTGAGAAAGAGT+GGG - chr1.4:30539943-30539962 MS.gene064944:intron 35.0%
GAGAAAGAGTGGGAATTAAT+GGG - chr1.4:30539953-30539972 MS.gene064944:intron 35.0%
GCTTACCATCATATGTTCTT+TGG + chr1.4:30540090-30540109 None:intergenic 35.0%
TGAGAAAGAGTGGGAATTAA+TGG - chr1.4:30539952-30539971 MS.gene064944:intron 35.0%
! ACTTGTTAAGGCAAAGTTTG+AGG - chr1.4:30540863-30540882 MS.gene064944:CDS 35.0%
! ATTAATGGGGACTAAGCTTT+TGG - chr1.4:30539967-30539986 MS.gene064944:intron 35.0%
!! AATGGTGTTTGCTTTTAAGG+AGG - chr1.4:30539808-30539827 MS.gene064944:CDS 35.0%
!! GCTTTTGGAAATATACTCAC+AGG - chr1.4:30540691-30540710 MS.gene064944:CDS 35.0%
AAAAGTAGTGTGTGGCAGAT+GGG - chr1.4:30540529-30540548 MS.gene064944:CDS 40.0%
AAGAGAGTAGGAACTATGAG+AGG - chr1.4:30539780-30539799 MS.gene064944:CDS 40.0%
AGAGAGTAGGAACTATGAGA+GGG - chr1.4:30539781-30539800 MS.gene064944:CDS 40.0%
ATTGGAAACAAGAGGTGAAG+AGG - chr1.4:30539834-30539853 MS.gene064944:CDS 40.0%
GAACTATGAGAGGGAGATAA+TGG - chr1.4:30539790-30539809 MS.gene064944:CDS 40.0%
GGAAATATACTCACAGGAGA+TGG - chr1.4:30540697-30540716 MS.gene064944:CDS 40.0%
TAGGTTCAGTGGAGAAAGAA+AGG - chr1.4:30540818-30540837 MS.gene064944:CDS 40.0%
! TCACAGGAGATGGTTTGTAT+TGG - chr1.4:30540707-30540726 MS.gene064944:CDS 40.0%
! TGGTTTGTATTGGAAAGCTG+AGG - chr1.4:30540717-30540736 MS.gene064944:CDS 40.0%
! TTTGGTTCAAGAGATGAAGG+AGG - chr1.4:30539985-30540004 MS.gene064944:intron 40.0%
!! GCCACACACTACTTTTCAAA+GGG + chr1.4:30540525-30540544 None:intergenic 40.0%
!! TGCCACACACTACTTTTCAA+AGG + chr1.4:30540526-30540545 None:intergenic 40.0%
!! TTGCTTTTAAGGAGGCAGAT+TGG - chr1.4:30539816-30539835 MS.gene064944:CDS 40.0%
!!! GCTTTTGGTTCAAGAGATGA+AGG - chr1.4:30539982-30540001 MS.gene064944:intron 40.0%
AAAGTAGTGTGTGGCAGATG+GGG - chr1.4:30540530-30540549 MS.gene064944:CDS 45.0%
AAGAAGAGGTGAAGCAGTTG+AGG - chr1.4:30539861-30539880 MS.gene064944:CDS 45.0%
AGAAGAGGTGAAGCAGTTGA+GGG - chr1.4:30539862-30539881 MS.gene064944:CDS 45.0%
AGATGAAGGAGGAAAGAGCA+AGG - chr1.4:30539996-30540015 MS.gene064944:intron 45.0%
ATAAGGGAGATGGAAGAGGT+TGG - chr1.4:30539911-30539930 MS.gene064944:intron 45.0%
GAAAAGTAGTGTGTGGCAGA+TGG - chr1.4:30540528-30540547 MS.gene064944:CDS 45.0%
GAAGATAAGGGAGATGGAAG+AGG - chr1.4:30539907-30539926 MS.gene064944:intron 45.0%
GAGATGAAGCTGTTGAGAAG+TGG - chr1.4:30540020-30540039 MS.gene064944:intron 45.0%
GGAAGAGAAGATAAGGGAGA+TGG - chr1.4:30539901-30539920 MS.gene064944:intron 45.0%
GGTGAAGAGGTTGAAAGAAG+AGG - chr1.4:30539847-30539866 MS.gene064944:CDS 45.0%
GTGATAGGGAGAAAGAGAGT+AGG - chr1.4:30539768-30539787 MS.gene064944:CDS 45.0%
! GCCCTTTGAAAAGTAGTGTG+TGG - chr1.4:30540521-30540540 MS.gene064944:CDS 45.0%
!!! AAAATTAAATAAAATTCTTT+TGG + chr1.4:30540376-30540395 None:intergenic 5.0%
GGAGATGGAAGAGGTTGGAA+TGG - chr1.4:30539916-30539935 MS.gene064944:intron 50.0%
GGAGGCAGATTGGAAACAAG+AGG - chr1.4:30539826-30539845 MS.gene064944:CDS 50.0%
GAAGCAGTTGAGGGAAGTGG+TGG - chr1.4:30539871-30539890 MS.gene064944:CDS 55.0%
GGTGAAGCAGTTGAGGGAAG+TGG - chr1.4:30539868-30539887 MS.gene064944:CDS 55.0%
! GAGGGAAGTGGTGGAAGAGA+AGG - chr1.4:30539880-30539899 MS.gene064944:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 30539653 30540957 30539653 ID=MS.gene064944
chr1.4 mRNA 30539653 30540957 30539653 ID=MS.gene064944.t1;Parent=MS.gene064944
chr1.4 exon 30540507 30540957 30540507 ID=MS.gene064944.t1.exon1;Parent=MS.gene064944.t1
chr1.4 CDS 30540507 30540957 30540507 ID=cds.MS.gene064944.t1;Parent=MS.gene064944.t1
chr1.4 exon 30539653 30539897 30539653 ID=MS.gene064944.t1.exon2;Parent=MS.gene064944.t1
chr1.4 CDS 30539653 30539897 30539653 ID=cds.MS.gene064944.t1;Parent=MS.gene064944.t1
Gene Sequence

>MS.gene064944

ATGGGGGGAAGTGTTAGTGTTAGCAAGAAAAATGAGATAAAGTATCCACAAAAATATGTAATGAATTTGAAAGAGAAAGTGAGACTTCTACAAGAAGAAATAAAGGAGATAATGTGTGATAGGGAGAAAGAGAGTAGGAACTATGAGAGGGAGATAATGGTGTTTGCTTTTAAGGAGGCAGATTGGAAACAAGAGGTGAAGAGGTTGAAAGAAGAGGTGAAGCAGTTGAGGGAAGTGGTGGAAGAGAAGGAAGAGAAGATAAGGGAGATGGAAGAGGTTGGAATGGTTGAGAAGAATTGTGAGAAAGAGTGGGAATTAATGGGGACTAAGCTTTTGGTTCAAGAGATGAAGGAGGAAAGAGCAAGGAGAGATGAAGCTGTTGAGAAGTGGAAACAACTTTATCTTGCAATTAAGAATGAGTTAGATGATCTCATCCAAAGAACATATGATGGTAAGCTAATCATAATTTGTTATTGATGTCTTAAGACATGTTTGAATTGAGTTATTTTAGCTTATTTATGAGCATGTTTGAGAGTTTATGAAAACAAATTATAATGTGTATAATCTATCTATTTTTATCTTATAAGTTGAATTGAGTTATTTTAGCTTATTTATGAGCATTTTTGAGAGAGTTTATAAAAATCAAATTATAATGTGTGTAATCTATTTTTATCTTATAAGTTTTCTAAAATAGCATATAAAAACAGTTTATAGTTTATGCCAAAAGAATTTTATTTAATTTTTTTTTGTAGAAATAGCTTATAAAATAATGATTATATAATAATTGTTTAATTAAGATTTTTATCCAAACAATTAGTACTGTTTGTATTTTTTTTGTGATTTGCATTAGTCTTTGTATGCTATTAATGCCCTTTGAAAAGTAGTGTGTGGCAGATGGGGTTCAATCCCATCATAGTACTTTTCAAAATTAGTCAATTAATATGTGACAATAATGTGGTGAATTATTTTTATTATCTTCTTGAGAAATATTCATACTACTATGCATGAATGCTATGTTTGAACTACTTGAGCTTATTTGCTTTTGGAAATATACTCACAGGAGATGGTTTGTATTGGAAAGCTGAGGAAAATGACATACAAATTGAAAATCTGAAGAGGGAATTGCAAGAGAAAGAAGAAAGCATAAAAGCTTTAAAAGCTCAATTAGGTTCAGTGGAGAAAGAAAGGTACAAACAAGAAAGGGAATTTGACTTGTTAAGGCAAAGTTTGAGGATCATGAATGGCAAGAAGAATCCAATTCAAACTAAAGAGAAGAAGAAGTTGAAAATCAAATTGGGAAAATAA

Protein sequence

>MS.gene064944.t1

MGGSVSVSKKNEIKYPQKYVMNLKEKVRLLQEEIKEIMCDREKESRNYEREIMVFAFKEADWKQEVKRLKEEVKQLREVVEEKEEKIREMEEVGMVEKNCEKEWELMGTKLLVQEMKEERARRDEAVEKWKQLYLAIKNELDDLIQRTYDGDGLYWKAEENDIQIENLKRELQEKEESIKALKAQLGSVEKERYKQEREFDLLRQSLRIMNGKKNPIQTKEKKKLKIKLGK