AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050325


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050325.t1 MTR_7g104490 99.284 419 3 0 1 419 1 419 0.0 845
MS.gene050325.t1 MTR_1g060040 71.360 419 120 0 1 419 1 419 0.0 589
MS.gene050325.t1 MTR_4g053260 63.723 419 152 0 1 419 2 420 0.0 556
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050325.t1 AT1G67190 63.007 419 155 0 1 419 1 419 0.0 528
MS.gene050325.t1 AT1G67190 63.007 419 155 0 1 419 1 419 0.0 528

Find 63 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTGACATGGCGATTTC+TGG 0.152383 7.2:-8322924 None:intergenic
TTCTCAATGATATTGAAATT+AGG 0.193329 7.2:-8322717 None:intergenic
AATCATGAATTCAGTGAATT+TGG 0.195737 7.2:-8323561 None:intergenic
TCTCAATGATATTGAAATTA+GGG 0.201362 7.2:-8322716 None:intergenic
AGAGCAAATTTCGCGATATA+GGG 0.320402 7.2:-8322526 None:intergenic
CATCCTTAACTTGCAAGAAA+TGG 0.324453 7.2:+8322448 MS.gene050325:CDS
AAAGATGGAGTGCTTCATTA+TGG 0.324477 7.2:+8323359 MS.gene050325:CDS
TAGAGCAAATTTCGCGATAT+AGG 0.327459 7.2:-8322527 None:intergenic
AGTTTGGGTAGCTGGACTTT+TGG 0.334910 7.2:+8323457 MS.gene050325:CDS
CTGTTACCCCTGTCATTGCT+TGG 0.349306 7.2:+8322640 MS.gene050325:CDS
ATGTATTGAATGAAAGAGTT+TGG 0.353897 7.2:-8322494 None:intergenic
ATAGACAAGGTTTCAAGTTT+CGG 0.358073 7.2:-8322894 None:intergenic
TCTCCATTTCTTGCAAGTTA+AGG 0.360964 7.2:-8322451 None:intergenic
ATCTTTCAAGTCATAACTTA+AGG 0.368328 7.2:-8323342 None:intergenic
GCTTAGCAGCTCTTCTTTGA+AGG 0.394764 7.2:+8322962 MS.gene050325:CDS
TTAAGTTATGACTTGAAAGA+TGG 0.408134 7.2:+8323344 MS.gene050325:CDS
CTTGGCTAGCAATCCTATCT+CGG 0.423380 7.2:+8322770 MS.gene050325:CDS
TGTTAGGCGCTGCAATCCTT+TGG 0.433874 7.2:-8322589 None:intergenic
GTATCATATTATCCCATCTT+GGG 0.437104 7.2:+8322403 MS.gene050325:CDS
GTGCTTCATTATGGCTTACA+AGG 0.443213 7.2:+8323368 MS.gene050325:CDS
CGAAATTTGCTCTAGTCACT+TGG 0.446849 7.2:+8322536 MS.gene050325:CDS
ATGATACCGACCACTTCAAC+AGG 0.448381 7.2:-8322387 None:intergenic
AAGGAAAGGTGCGTTAATCT+AGG 0.450642 7.2:-8323323 None:intergenic
TACATAAGCCAAGCAATGAC+AGG 0.467063 7.2:-8322648 None:intergenic
TATCCCATCTTGGGTCTGCT+AGG 0.472254 7.2:+8322412 MS.gene050325:CDS
GGTATCATATTATCCCATCT+TGG 0.475174 7.2:+8322402 MS.gene050325:CDS
ATCCCATCTTGGGTCTGCTA+GGG 0.476732 7.2:+8322413 MS.gene050325:CDS
CATCATCCATGAAAATTGTT+AGG 0.477751 7.2:-8322605 None:intergenic
TCTAGGAAAACAAACAGATA+TGG 0.480581 7.2:-8323306 None:intergenic
TTCGGCTCAACACCCGAGAT+AGG 0.481385 7.2:-8322783 None:intergenic
CATCCCTAGCAGACCCAAGA+TGG 0.487907 7.2:-8322416 None:intergenic
AATGTGGTTGTGTTAGAACT+TGG 0.510674 7.2:+8323407 MS.gene050325:CDS
CAAGGAAATTTATGATACTT+CGG 0.514673 7.2:-8322801 None:intergenic
GTTCGAGTTTGAATATGAGT+AGG 0.522810 7.2:+8323613 MS.gene050325:CDS
GATCAATTCAAAAGCATCAA+AGG 0.535278 7.2:-8323066 None:intergenic
TTGGCTAGCAATCCTATCTC+GGG 0.539654 7.2:+8322771 MS.gene050325:CDS
CTCAATGATATTGAAATTAG+GGG 0.540853 7.2:-8322715 None:intergenic
TGTGTAACTTCATATTTATG+TGG 0.543217 7.2:+8323183 MS.gene050325:CDS
TCAAGTCATAACTTAAGGAA+AGG 0.545749 7.2:-8323337 None:intergenic
TATTATCTCCAATGTCAAGA+TGG 0.549282 7.2:-8323136 None:intergenic
GCAAGAAATGGAGAGAAGCT+TGG 0.560253 7.2:+8322460 MS.gene050325:CDS
AGTGTCATCCATCTTGACAT+TGG 0.562714 7.2:+8323128 MS.gene050325:CDS
TACACACATCTACGATCTCA+AGG 0.565105 7.2:-8323166 None:intergenic
TTTCATTCAATACATCTGAC+TGG 0.565276 7.2:+8322502 MS.gene050325:CDS
AATCGCCATGTCAGATTCAG+AGG 0.567446 7.2:+8322929 MS.gene050325:CDS
ACTGAATTCATGATTCAACT+AGG 0.580130 7.2:+8323569 MS.gene050325:CDS
ATCCCTAGCAGACCCAAGAT+GGG 0.587597 7.2:-8322415 None:intergenic
TGGTTGTGTTAGAACTTGGA+TGG 0.588684 7.2:+8323411 MS.gene050325:CDS
GAGCAAATTTCGCGATATAG+GGG 0.592223 7.2:-8322525 None:intergenic
GGAGGATCTTCCTGTTGAAG+TGG 0.592625 7.2:+8322377 MS.gene050325:CDS
AACGACAGCGACTTCAAGCA+AGG 0.592920 7.2:-8322819 None:intergenic
ATATCATTGAGAAATGCAGC+AGG 0.597773 7.2:+8322727 MS.gene050325:CDS
TTCTGGACAAACAATAGACA+AGG 0.600718 7.2:-8322907 None:intergenic
TGCTTCATTATGGCTTACAA+GGG 0.605298 7.2:+8323369 MS.gene050325:CDS
TGCTTGGCTTATGTATACGA+GGG 0.608384 7.2:+8322656 MS.gene050325:CDS
TATTGAATGAAAGAGTTTGG+AGG 0.609653 7.2:-8322491 None:intergenic
TGATAAACTCATATTAGTGG+CGG 0.615767 7.2:+8323010 MS.gene050325:CDS
ACATAAGCCAAGCAATGACA+GGG 0.628082 7.2:-8322647 None:intergenic
GATCTTCCTGTTGAAGTGGT+CGG 0.634333 7.2:+8322381 MS.gene050325:CDS
TGAAGCCTCTGAATCTGACA+TGG 0.638655 7.2:-8322934 None:intergenic
TTGCTTGGCTTATGTATACG+AGG 0.648455 7.2:+8322655 MS.gene050325:CDS
CGAGATAGGATTGCTAGCCA+AGG 0.649589 7.2:-8322769 None:intergenic
CATAAGCCAAGCAATGACAG+GGG 0.741525 7.2:-8322646 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTCAATGATATTGAAATTA+GGG - chr7.2:8322719-8322738 None:intergenic 20.0%
!! TTCTCAATGATATTGAAATT+AGG - chr7.2:8322720-8322739 None:intergenic 20.0%
!!! TTTTGATAAACTCATATTAG+TGG + chr7.2:8323007-8323026 MS.gene050325:CDS 20.0%
! AATCATGAATTCAGTGAATT+TGG - chr7.2:8323564-8323583 None:intergenic 25.0%
! ATGTATTGAATGAAAGAGTT+TGG - chr7.2:8322497-8322516 None:intergenic 25.0%
! ATGTCCTAATCTTAAAAAGA+TGG + chr7.2:8323484-8323503 MS.gene050325:CDS 25.0%
! CAAGGAAATTTATGATACTT+CGG - chr7.2:8322804-8322823 None:intergenic 25.0%
! CTCAATGATATTGAAATTAG+GGG - chr7.2:8322718-8322737 None:intergenic 25.0%
! CTTAAAAAGATGGTCATTTA+TGG + chr7.2:8323494-8323513 MS.gene050325:CDS 25.0%
! TGTGTAACTTCATATTTATG+TGG + chr7.2:8323183-8323202 MS.gene050325:CDS 25.0%
! TTAAGTTATGACTTGAAAGA+TGG + chr7.2:8323344-8323363 MS.gene050325:CDS 25.0%
!! ATCTTTCAAGTCATAACTTA+AGG - chr7.2:8323345-8323364 None:intergenic 25.0%
!! CTTTTGAATTGATCAATAAG+GGG + chr7.2:8323075-8323094 MS.gene050325:CDS 25.0%
!! GCTTTTGAATTGATCAATAA+GGG + chr7.2:8323074-8323093 MS.gene050325:CDS 25.0%
!! TGCTTTTGAATTGATCAATA+AGG + chr7.2:8323073-8323092 MS.gene050325:CDS 25.0%
!!! ATGACCATCTTTTTAAGATT+AGG - chr7.2:8323491-8323510 None:intergenic 25.0%
ACTGAATTCATGATTCAACT+AGG + chr7.2:8323569-8323588 MS.gene050325:CDS 30.0%
CATCATCCATGAAAATTGTT+AGG - chr7.2:8322608-8322627 None:intergenic 30.0%
CGTCAAAGTTAAAAAAGCTT+CGG + chr7.2:8323234-8323253 MS.gene050325:CDS 30.0%
GTATCATATTATCCCATCTT+GGG + chr7.2:8322403-8322422 MS.gene050325:CDS 30.0%
TATTATCTCCAATGTCAAGA+TGG - chr7.2:8323139-8323158 None:intergenic 30.0%
TATTGAATGAAAGAGTTTGG+AGG - chr7.2:8322494-8322513 None:intergenic 30.0%
TCAAGTCATAACTTAAGGAA+AGG - chr7.2:8323340-8323359 None:intergenic 30.0%
TCTAGGAAAACAAACAGATA+TGG - chr7.2:8323309-8323328 None:intergenic 30.0%
TGATAAACTCATATTAGTGG+CGG + chr7.2:8323010-8323029 MS.gene050325:CDS 30.0%
TTTCATTCAATACATCTGAC+TGG + chr7.2:8322502-8322521 MS.gene050325:CDS 30.0%
!! ATAGACAAGGTTTCAAGTTT+CGG - chr7.2:8322897-8322916 None:intergenic 30.0%
!! GATCAATTCAAAAGCATCAA+AGG - chr7.2:8323069-8323088 None:intergenic 30.0%
!!! TGTACACTTTTTCAAACCAA+AGG + chr7.2:8322573-8322592 MS.gene050325:CDS 30.0%
AATGTGGTTGTGTTAGAACT+TGG + chr7.2:8323407-8323426 MS.gene050325:CDS 35.0%
AGAGCAAATTTCGCGATATA+GGG - chr7.2:8322529-8322548 None:intergenic 35.0%
ATATCATTGAGAAATGCAGC+AGG + chr7.2:8322727-8322746 MS.gene050325:CDS 35.0%
GGTATCATATTATCCCATCT+TGG + chr7.2:8322402-8322421 MS.gene050325:CDS 35.0%
TAGAGCAAATTTCGCGATAT+AGG - chr7.2:8322530-8322549 None:intergenic 35.0%
TCTCCATTTCTTGCAAGTTA+AGG - chr7.2:8322454-8322473 None:intergenic 35.0%
TGCTTCATTATGGCTTACAA+GGG + chr7.2:8323369-8323388 MS.gene050325:CDS 35.0%
TTCTGGACAAACAATAGACA+AGG - chr7.2:8322910-8322929 None:intergenic 35.0%
! AAAGATGGAGTGCTTCATTA+TGG + chr7.2:8323359-8323378 MS.gene050325:CDS 35.0%
! CAGTGATCTTTTCTCAGTTT+GGG + chr7.2:8323442-8323461 MS.gene050325:CDS 35.0%
! GTTGTCTTTTCTGATGAATG+TGG + chr7.2:8323391-8323410 MS.gene050325:CDS 35.0%
! TCAGTGATCTTTTCTCAGTT+TGG + chr7.2:8323441-8323460 MS.gene050325:CDS 35.0%
!! CATCCTTAACTTGCAAGAAA+TGG + chr7.2:8322448-8322467 MS.gene050325:CDS 35.0%
ACATAAGCCAAGCAATGACA+GGG - chr7.2:8322650-8322669 None:intergenic 40.0%
AGTGTCATCCATCTTGACAT+TGG + chr7.2:8323128-8323147 MS.gene050325:CDS 40.0%
AGTTAAAAAAGCTTCGGCTG+TGG + chr7.2:8323240-8323259 MS.gene050325:CDS 40.0%
GAAATGCAGCAGGAAAAAGT+TGG + chr7.2:8322737-8322756 MS.gene050325:CDS 40.0%
GAGCAAATTTCGCGATATAG+GGG - chr7.2:8322528-8322547 None:intergenic 40.0%
GTGCTTCATTATGGCTTACA+AGG + chr7.2:8323368-8323387 MS.gene050325:CDS 40.0%
TACACACATCTACGATCTCA+AGG - chr7.2:8323169-8323188 None:intergenic 40.0%
TACATAAGCCAAGCAATGAC+AGG - chr7.2:8322651-8322670 None:intergenic 40.0%
TGCTTGGCTTATGTATACGA+GGG + chr7.2:8322656-8322675 MS.gene050325:CDS 40.0%
TGGTTGTGTTAGAACTTGGA+TGG + chr7.2:8323411-8323430 MS.gene050325:CDS 40.0%
TTGCTTGGCTTATGTATACG+AGG + chr7.2:8322655-8322674 MS.gene050325:CDS 40.0%
! AAGGAAAGGTGCGTTAATCT+AGG - chr7.2:8323326-8323345 None:intergenic 40.0%
! CGAAATTTGCTCTAGTCACT+TGG + chr7.2:8322536-8322555 MS.gene050325:CDS 40.0%
! TTTTGTTAGCATCTCAGCGT+TGG + chr7.2:8322845-8322864 MS.gene050325:CDS 40.0%
!! AAAGTTGGAGGTTTTGACCT+TGG + chr7.2:8322752-8322771 MS.gene050325:CDS 40.0%
AATCGCCATGTCAGATTCAG+AGG + chr7.2:8322929-8322948 MS.gene050325:CDS 45.0%
ATGATACCGACCACTTCAAC+AGG - chr7.2:8322390-8322409 None:intergenic 45.0%
ATGCAGCAGGAAAAAGTTGG+AGG + chr7.2:8322740-8322759 MS.gene050325:CDS 45.0%
CATAAGCCAAGCAATGACAG+GGG - chr7.2:8322649-8322668 None:intergenic 45.0%
CTTGGCTAGCAATCCTATCT+CGG + chr7.2:8322770-8322789 MS.gene050325:CDS 45.0%
GAATCTGACATGGCGATTTC+TGG - chr7.2:8322927-8322946 None:intergenic 45.0%
GATCTTCCTGTTGAAGTGGT+CGG + chr7.2:8322381-8322400 MS.gene050325:CDS 45.0%
GCAAGAAATGGAGAGAAGCT+TGG + chr7.2:8322460-8322479 MS.gene050325:CDS 45.0%
GCTTAGCAGCTCTTCTTTGA+AGG + chr7.2:8322962-8322981 MS.gene050325:CDS 45.0%
TGAAGCCTCTGAATCTGACA+TGG - chr7.2:8322937-8322956 None:intergenic 45.0%
TTGGCTAGCAATCCTATCTC+GGG + chr7.2:8322771-8322790 MS.gene050325:CDS 45.0%
! AGTTTGGGTAGCTGGACTTT+TGG + chr7.2:8323457-8323476 MS.gene050325:CDS 45.0%
! CTTTTCTCAGTTTGGGTAGC+TGG + chr7.2:8323449-8323468 MS.gene050325:CDS 45.0%
! GCAGCGCCTAACAATTTTCA+TGG + chr7.2:8322599-8322618 MS.gene050325:CDS 45.0%
!! TGTTTTTGACGACGAGGATG+AGG + chr7.2:8323268-8323287 MS.gene050325:CDS 45.0%
!!! GAGTGTTGTTTTTGACGACG+AGG + chr7.2:8323262-8323281 MS.gene050325:CDS 45.0%
AACGACAGCGACTTCAAGCA+AGG - chr7.2:8322822-8322841 None:intergenic 50.0%
ATCCCATCTTGGGTCTGCTA+GGG + chr7.2:8322413-8322432 MS.gene050325:CDS 50.0%
ATCCCTAGCAGACCCAAGAT+GGG - chr7.2:8322418-8322437 None:intergenic 50.0%
CGAGATAGGATTGCTAGCCA+AGG - chr7.2:8322772-8322791 None:intergenic 50.0%
CTGTTACCCCTGTCATTGCT+TGG + chr7.2:8322640-8322659 MS.gene050325:CDS 50.0%
GGAGGATCTTCCTGTTGAAG+TGG + chr7.2:8322377-8322396 MS.gene050325:CDS 50.0%
TATCCCATCTTGGGTCTGCT+AGG + chr7.2:8322412-8322431 MS.gene050325:CDS 50.0%
! TGGGTAGCTGGACTTTTGGA+AGG + chr7.2:8323461-8323480 MS.gene050325:CDS 50.0%
! TGTTAGGCGCTGCAATCCTT+TGG - chr7.2:8322592-8322611 None:intergenic 50.0%
CATCCCTAGCAGACCCAAGA+TGG - chr7.2:8322419-8322438 None:intergenic 55.0%
TTCGGCTCAACACCCGAGAT+AGG - chr7.2:8322786-8322805 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 8322375 8323634 8322375 ID=MS.gene050325
chr7.2 mRNA 8322375 8323634 8322375 ID=MS.gene050325.t1;Parent=MS.gene050325
chr7.2 exon 8322375 8323634 8322375 ID=MS.gene050325.t1.exon1;Parent=MS.gene050325.t1
chr7.2 CDS 8322375 8323634 8322375 ID=cds.MS.gene050325.t1;Parent=MS.gene050325.t1
Gene Sequence

>MS.gene050325

ATGGAGGATCTTCCTGTTGAAGTGGTCGGTATCATATTATCCCATCTTGGGTCTGCTAGGGATGTTCTGATTGCATCCTTAACTTGCAAGAAATGGAGAGAAGCTTGGCAGAATCACCTCCAAACTCTTTCATTCAATACATCTGACTGGCCCCTATATCGCGAAATTTGCTCTAGTCACTTGGAAATGCTCATTACTTGTACACTTTTTCAAACCAAAGGATTGCAGCGCCTAACAATTTTCATGGATGATGAGCATGAGTTTTCTGTTACCCCTGTCATTGCTTGGCTTATGTATACGAGGGATTCATTGCGCGAGCTACGTTATAATGTGAGAACATCCCCTAATTTCAATATCATTGAGAAATGCAGCAGGAAAAAGTTGGAGGTTTTGACCTTGGCTAGCAATCCTATCTCGGGTGTTGAGCCGAAGTATCATAAATTTCCTTGCTTGAAGTCGCTGTCGTTGAGTTTTGTTAGCATCTCAGCGTTGGATTTGAGTCTTCTGCTTTCTGCTTGTCCGAAACTTGAAACCTTGTCTATTGTTTGTCCAGAAATCGCCATGTCAGATTCAGAGGCTTCAATAGAGCTTAGCAGCTCTTCTTTGAAGGATTTCTTTGTCGAGTCATATAGTTTTGATAAACTCATATTAGTGGCGGATATGCTCGAGAATCTGCATCTGAAAGATTGTTCCTTTGATGCTTTTGAATTGATCAATAAGGGGACATTGAAAGTATTGAAGCTTGATGATGTTAGTGTCATCCATCTTGACATTGGAGATAATACAGAAAACCTTGAGATCGTAGATGTGTGTAACTTCATATTTATGTGGCAAAATTTTTATAATATGATCTCAAAAGCGTCAAAGTTAAAAAAGCTTCGGCTGTGGAGTGTTGTTTTTGACGACGAGGATGAGGTTGTTGATATAGAGACCATATCTGTTTGTTTTCCTAGATTAACGCACCTTTCCTTAAGTTATGACTTGAAAGATGGAGTGCTTCATTATGGCTTACAAGGGTTGTCTTTTCTGATGAATGTGGTTGTGTTAGAACTTGGATGGACTACAATCAGTGATCTTTTCTCAGTTTGGGTAGCTGGACTTTTGGAAGGATGTCCTAATCTTAAAAAGATGGTCATTTATGGCTATGTTGCTGAGATCAAAACACATGAAGAGTGTCAAACTTTTACCAAATTCACTGAATTCATGATTCAACTAGGAAGAAAATATAGTCATATAAAGTTCGAGTTTGAATATGAGTAG

Protein sequence

>MS.gene050325.t1

MEDLPVEVVGIILSHLGSARDVLIASLTCKKWREAWQNHLQTLSFNTSDWPLYREICSSHLEMLITCTLFQTKGLQRLTIFMDDEHEFSVTPVIAWLMYTRDSLRELRYNVRTSPNFNIIEKCSRKKLEVLTLASNPISGVEPKYHKFPCLKSLSLSFVSISALDLSLLLSACPKLETLSIVCPEIAMSDSEASIELSSSSLKDFFVESYSFDKLILVADMLENLHLKDCSFDAFELINKGTLKVLKLDDVSVIHLDIGDNTENLEIVDVCNFIFMWQNFYNMISKASKLKKLRLWSVVFDDEDEVVDIETISVCFPRLTHLSLSYDLKDGVLHYGLQGLSFLMNVVVLELGWTTISDLFSVWVAGLLEGCPNLKKMVIYGYVAEIKTHEECQTFTKFTEFMIQLGRKYSHIKFEFEYE