AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050327


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050327.t1 MTR_7g104490 99.284 419 3 0 1 419 1 419 0.0 845
MS.gene050327.t1 MTR_1g060040 71.360 419 120 0 1 419 1 419 0.0 589
MS.gene050327.t1 MTR_4g053260 63.723 419 152 0 1 419 2 420 0.0 556
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050327.t1 AT1G67190 63.007 419 155 0 1 419 1 419 0.0 528
MS.gene050327.t1 AT1G67190 63.007 419 155 0 1 419 1 419 0.0 528

Find 63 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTGACATGGCGATTTC+TGG 0.152383 7.2:-8333300 None:intergenic
TTCTCAATGATATTGAAATT+AGG 0.193329 7.2:-8333093 None:intergenic
AATCATGAATTCAGTGAATT+TGG 0.195737 7.2:-8333937 None:intergenic
TCTCAATGATATTGAAATTA+GGG 0.201362 7.2:-8333092 None:intergenic
AGAGCAAATTTCGCGATATA+GGG 0.320402 7.2:-8332902 None:intergenic
CATCCTTAACTTGCAAGAAA+TGG 0.324453 7.2:+8332824 MS.gene050327:CDS
AAAGATGGAGTGCTTCATTA+TGG 0.324477 7.2:+8333735 MS.gene050327:CDS
TAGAGCAAATTTCGCGATAT+AGG 0.327459 7.2:-8332903 None:intergenic
AGTTTGGGTAGCTGGACTTT+TGG 0.334910 7.2:+8333833 MS.gene050327:CDS
CTGTTACCCCTGTCATTGCT+TGG 0.349306 7.2:+8333016 MS.gene050327:CDS
ATGTATTGAATGAAAGAGTT+TGG 0.353897 7.2:-8332870 None:intergenic
ATAGACAAGGTTTCAAGTTT+CGG 0.358073 7.2:-8333270 None:intergenic
TCTCCATTTCTTGCAAGTTA+AGG 0.360964 7.2:-8332827 None:intergenic
ATCTTTCAAGTCATAACTTA+AGG 0.368328 7.2:-8333718 None:intergenic
GCTTAGCAGCTCTTCTTTGA+AGG 0.394764 7.2:+8333338 MS.gene050327:CDS
TTAAGTTATGACTTGAAAGA+TGG 0.408134 7.2:+8333720 MS.gene050327:CDS
CTTGGCTAGCAATCCTATCT+CGG 0.423380 7.2:+8333146 MS.gene050327:CDS
TGTTAGGCGCTGCAATCCTT+TGG 0.433874 7.2:-8332965 None:intergenic
GTATCATATTATCCCATCTT+GGG 0.437104 7.2:+8332779 MS.gene050327:CDS
GTGCTTCATTATGGCTTACA+AGG 0.443213 7.2:+8333744 MS.gene050327:CDS
CGAAATTTGCTCTAGTCACT+TGG 0.446849 7.2:+8332912 MS.gene050327:CDS
ATGATACCGACCACTTCAAC+AGG 0.448381 7.2:-8332763 None:intergenic
AAGGAAAGGTGCGTTAATCT+AGG 0.450642 7.2:-8333699 None:intergenic
TACATAAGCCAAGCAATGAC+AGG 0.467063 7.2:-8333024 None:intergenic
TATCCCATCTTGGGTCTGCT+AGG 0.472254 7.2:+8332788 MS.gene050327:CDS
GGTATCATATTATCCCATCT+TGG 0.475174 7.2:+8332778 MS.gene050327:CDS
ATCCCATCTTGGGTCTGCTA+GGG 0.476732 7.2:+8332789 MS.gene050327:CDS
CATCATCCATGAAAATTGTT+AGG 0.477751 7.2:-8332981 None:intergenic
TCTAGGAAAACAAACAGATA+TGG 0.480581 7.2:-8333682 None:intergenic
TTCGGCTCAACACCCGAGAT+AGG 0.481385 7.2:-8333159 None:intergenic
CATCCCTAGCAGACCCAAGA+TGG 0.487907 7.2:-8332792 None:intergenic
AATGTGGTTGTGTTAGAACT+TGG 0.510674 7.2:+8333783 MS.gene050327:CDS
CAAGGAAATTTATGATACTT+CGG 0.514673 7.2:-8333177 None:intergenic
GTTCGAGTTTGAATATGAGT+AGG 0.522810 7.2:+8333989 MS.gene050327:CDS
GATCAATTCAAAAGCATCAA+AGG 0.535278 7.2:-8333442 None:intergenic
TTGGCTAGCAATCCTATCTC+GGG 0.539654 7.2:+8333147 MS.gene050327:CDS
CTCAATGATATTGAAATTAG+GGG 0.540853 7.2:-8333091 None:intergenic
TGTGTAACTTCATATTTATG+TGG 0.543217 7.2:+8333559 MS.gene050327:CDS
TCAAGTCATAACTTAAGGAA+AGG 0.545749 7.2:-8333713 None:intergenic
TATTATCTCCAATGTCAAGA+TGG 0.549282 7.2:-8333512 None:intergenic
GCAAGAAATGGAGAGAAGCT+TGG 0.560253 7.2:+8332836 MS.gene050327:CDS
AGTGTCATCCATCTTGACAT+TGG 0.562714 7.2:+8333504 MS.gene050327:CDS
TACACACATCTACGATCTCA+AGG 0.565105 7.2:-8333542 None:intergenic
TTTCATTCAATACATCTGAC+TGG 0.565276 7.2:+8332878 MS.gene050327:CDS
AATCGCCATGTCAGATTCAG+AGG 0.567446 7.2:+8333305 MS.gene050327:CDS
ACTGAATTCATGATTCAACT+AGG 0.580130 7.2:+8333945 MS.gene050327:CDS
ATCCCTAGCAGACCCAAGAT+GGG 0.587597 7.2:-8332791 None:intergenic
TGGTTGTGTTAGAACTTGGA+TGG 0.588684 7.2:+8333787 MS.gene050327:CDS
GAGCAAATTTCGCGATATAG+GGG 0.592223 7.2:-8332901 None:intergenic
GGAGGATCTTCCTGTTGAAG+TGG 0.592625 7.2:+8332753 MS.gene050327:CDS
AACGACAGCGACTTCAAGCA+AGG 0.592920 7.2:-8333195 None:intergenic
ATATCATTGAGAAATGCAGC+AGG 0.597773 7.2:+8333103 MS.gene050327:CDS
TTCTGGACAAACAATAGACA+AGG 0.600718 7.2:-8333283 None:intergenic
TGCTTCATTATGGCTTACAA+GGG 0.605298 7.2:+8333745 MS.gene050327:CDS
TGCTTGGCTTATGTATACGA+GGG 0.608384 7.2:+8333032 MS.gene050327:CDS
TATTGAATGAAAGAGTTTGG+AGG 0.609653 7.2:-8332867 None:intergenic
TGATAAACTCATATTAGTGG+CGG 0.615767 7.2:+8333386 MS.gene050327:CDS
ACATAAGCCAAGCAATGACA+GGG 0.628082 7.2:-8333023 None:intergenic
GATCTTCCTGTTGAAGTGGT+CGG 0.634333 7.2:+8332757 MS.gene050327:CDS
TGAAGCCTCTGAATCTGACA+TGG 0.638655 7.2:-8333310 None:intergenic
TTGCTTGGCTTATGTATACG+AGG 0.648455 7.2:+8333031 MS.gene050327:CDS
CGAGATAGGATTGCTAGCCA+AGG 0.649589 7.2:-8333145 None:intergenic
CATAAGCCAAGCAATGACAG+GGG 0.741525 7.2:-8333022 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTCAATGATATTGAAATTA+GGG - chr7.2:8333095-8333114 None:intergenic 20.0%
!! TTCTCAATGATATTGAAATT+AGG - chr7.2:8333096-8333115 None:intergenic 20.0%
!!! TTTTGATAAACTCATATTAG+TGG + chr7.2:8333383-8333402 MS.gene050327:CDS 20.0%
! AATCATGAATTCAGTGAATT+TGG - chr7.2:8333940-8333959 None:intergenic 25.0%
! ATGTATTGAATGAAAGAGTT+TGG - chr7.2:8332873-8332892 None:intergenic 25.0%
! ATGTCCTAATCTTAAAAAGA+TGG + chr7.2:8333860-8333879 MS.gene050327:CDS 25.0%
! CAAGGAAATTTATGATACTT+CGG - chr7.2:8333180-8333199 None:intergenic 25.0%
! CTCAATGATATTGAAATTAG+GGG - chr7.2:8333094-8333113 None:intergenic 25.0%
! CTTAAAAAGATGGTCATTTA+TGG + chr7.2:8333870-8333889 MS.gene050327:CDS 25.0%
! TGTGTAACTTCATATTTATG+TGG + chr7.2:8333559-8333578 MS.gene050327:CDS 25.0%
! TTAAGTTATGACTTGAAAGA+TGG + chr7.2:8333720-8333739 MS.gene050327:CDS 25.0%
!! ATCTTTCAAGTCATAACTTA+AGG - chr7.2:8333721-8333740 None:intergenic 25.0%
!! CTTTTGAATTGATCAATAAG+GGG + chr7.2:8333451-8333470 MS.gene050327:CDS 25.0%
!! GCTTTTGAATTGATCAATAA+GGG + chr7.2:8333450-8333469 MS.gene050327:CDS 25.0%
!! TGCTTTTGAATTGATCAATA+AGG + chr7.2:8333449-8333468 MS.gene050327:CDS 25.0%
!!! ATGACCATCTTTTTAAGATT+AGG - chr7.2:8333867-8333886 None:intergenic 25.0%
ACTGAATTCATGATTCAACT+AGG + chr7.2:8333945-8333964 MS.gene050327:CDS 30.0%
CATCATCCATGAAAATTGTT+AGG - chr7.2:8332984-8333003 None:intergenic 30.0%
CGTCAAAGTTAAAAAAGCTT+CGG + chr7.2:8333610-8333629 MS.gene050327:CDS 30.0%
GTATCATATTATCCCATCTT+GGG + chr7.2:8332779-8332798 MS.gene050327:CDS 30.0%
TATTATCTCCAATGTCAAGA+TGG - chr7.2:8333515-8333534 None:intergenic 30.0%
TATTGAATGAAAGAGTTTGG+AGG - chr7.2:8332870-8332889 None:intergenic 30.0%
TCAAGTCATAACTTAAGGAA+AGG - chr7.2:8333716-8333735 None:intergenic 30.0%
TCTAGGAAAACAAACAGATA+TGG - chr7.2:8333685-8333704 None:intergenic 30.0%
TGATAAACTCATATTAGTGG+CGG + chr7.2:8333386-8333405 MS.gene050327:CDS 30.0%
TTTCATTCAATACATCTGAC+TGG + chr7.2:8332878-8332897 MS.gene050327:CDS 30.0%
!! ATAGACAAGGTTTCAAGTTT+CGG - chr7.2:8333273-8333292 None:intergenic 30.0%
!! GATCAATTCAAAAGCATCAA+AGG - chr7.2:8333445-8333464 None:intergenic 30.0%
!!! TGTACACTTTTTCAAACCAA+AGG + chr7.2:8332949-8332968 MS.gene050327:CDS 30.0%
AATGTGGTTGTGTTAGAACT+TGG + chr7.2:8333783-8333802 MS.gene050327:CDS 35.0%
AGAGCAAATTTCGCGATATA+GGG - chr7.2:8332905-8332924 None:intergenic 35.0%
ATATCATTGAGAAATGCAGC+AGG + chr7.2:8333103-8333122 MS.gene050327:CDS 35.0%
GGTATCATATTATCCCATCT+TGG + chr7.2:8332778-8332797 MS.gene050327:CDS 35.0%
TAGAGCAAATTTCGCGATAT+AGG - chr7.2:8332906-8332925 None:intergenic 35.0%
TCTCCATTTCTTGCAAGTTA+AGG - chr7.2:8332830-8332849 None:intergenic 35.0%
TGCTTCATTATGGCTTACAA+GGG + chr7.2:8333745-8333764 MS.gene050327:CDS 35.0%
TTCTGGACAAACAATAGACA+AGG - chr7.2:8333286-8333305 None:intergenic 35.0%
! AAAGATGGAGTGCTTCATTA+TGG + chr7.2:8333735-8333754 MS.gene050327:CDS 35.0%
! CAGTGATCTTTTCTCAGTTT+GGG + chr7.2:8333818-8333837 MS.gene050327:CDS 35.0%
! GTTGTCTTTTCTGATGAATG+TGG + chr7.2:8333767-8333786 MS.gene050327:CDS 35.0%
! TCAGTGATCTTTTCTCAGTT+TGG + chr7.2:8333817-8333836 MS.gene050327:CDS 35.0%
!! CATCCTTAACTTGCAAGAAA+TGG + chr7.2:8332824-8332843 MS.gene050327:CDS 35.0%
ACATAAGCCAAGCAATGACA+GGG - chr7.2:8333026-8333045 None:intergenic 40.0%
AGTGTCATCCATCTTGACAT+TGG + chr7.2:8333504-8333523 MS.gene050327:CDS 40.0%
AGTTAAAAAAGCTTCGGCTG+TGG + chr7.2:8333616-8333635 MS.gene050327:CDS 40.0%
GAAATGCAGCAGGAAAAAGT+TGG + chr7.2:8333113-8333132 MS.gene050327:CDS 40.0%
GAGCAAATTTCGCGATATAG+GGG - chr7.2:8332904-8332923 None:intergenic 40.0%
GTGCTTCATTATGGCTTACA+AGG + chr7.2:8333744-8333763 MS.gene050327:CDS 40.0%
TACACACATCTACGATCTCA+AGG - chr7.2:8333545-8333564 None:intergenic 40.0%
TACATAAGCCAAGCAATGAC+AGG - chr7.2:8333027-8333046 None:intergenic 40.0%
TGCTTGGCTTATGTATACGA+GGG + chr7.2:8333032-8333051 MS.gene050327:CDS 40.0%
TGGTTGTGTTAGAACTTGGA+TGG + chr7.2:8333787-8333806 MS.gene050327:CDS 40.0%
TTGCTTGGCTTATGTATACG+AGG + chr7.2:8333031-8333050 MS.gene050327:CDS 40.0%
! AAGGAAAGGTGCGTTAATCT+AGG - chr7.2:8333702-8333721 None:intergenic 40.0%
! CGAAATTTGCTCTAGTCACT+TGG + chr7.2:8332912-8332931 MS.gene050327:CDS 40.0%
! TTTTGTTAGCATCTCAGCGT+TGG + chr7.2:8333221-8333240 MS.gene050327:CDS 40.0%
!! AAAGTTGGAGGTTTTGACCT+TGG + chr7.2:8333128-8333147 MS.gene050327:CDS 40.0%
AATCGCCATGTCAGATTCAG+AGG + chr7.2:8333305-8333324 MS.gene050327:CDS 45.0%
ATGATACCGACCACTTCAAC+AGG - chr7.2:8332766-8332785 None:intergenic 45.0%
ATGCAGCAGGAAAAAGTTGG+AGG + chr7.2:8333116-8333135 MS.gene050327:CDS 45.0%
CATAAGCCAAGCAATGACAG+GGG - chr7.2:8333025-8333044 None:intergenic 45.0%
CTTGGCTAGCAATCCTATCT+CGG + chr7.2:8333146-8333165 MS.gene050327:CDS 45.0%
GAATCTGACATGGCGATTTC+TGG - chr7.2:8333303-8333322 None:intergenic 45.0%
GATCTTCCTGTTGAAGTGGT+CGG + chr7.2:8332757-8332776 MS.gene050327:CDS 45.0%
GCAAGAAATGGAGAGAAGCT+TGG + chr7.2:8332836-8332855 MS.gene050327:CDS 45.0%
GCTTAGCAGCTCTTCTTTGA+AGG + chr7.2:8333338-8333357 MS.gene050327:CDS 45.0%
TGAAGCCTCTGAATCTGACA+TGG - chr7.2:8333313-8333332 None:intergenic 45.0%
TTGGCTAGCAATCCTATCTC+GGG + chr7.2:8333147-8333166 MS.gene050327:CDS 45.0%
! AGTTTGGGTAGCTGGACTTT+TGG + chr7.2:8333833-8333852 MS.gene050327:CDS 45.0%
! CTTTTCTCAGTTTGGGTAGC+TGG + chr7.2:8333825-8333844 MS.gene050327:CDS 45.0%
! GCAGCGCCTAACAATTTTCA+TGG + chr7.2:8332975-8332994 MS.gene050327:CDS 45.0%
!! TGTTTTTGACGACGAGGATG+AGG + chr7.2:8333644-8333663 MS.gene050327:CDS 45.0%
!!! GAGTGTTGTTTTTGACGACG+AGG + chr7.2:8333638-8333657 MS.gene050327:CDS 45.0%
AACGACAGCGACTTCAAGCA+AGG - chr7.2:8333198-8333217 None:intergenic 50.0%
ATCCCATCTTGGGTCTGCTA+GGG + chr7.2:8332789-8332808 MS.gene050327:CDS 50.0%
ATCCCTAGCAGACCCAAGAT+GGG - chr7.2:8332794-8332813 None:intergenic 50.0%
CGAGATAGGATTGCTAGCCA+AGG - chr7.2:8333148-8333167 None:intergenic 50.0%
CTGTTACCCCTGTCATTGCT+TGG + chr7.2:8333016-8333035 MS.gene050327:CDS 50.0%
GGAGGATCTTCCTGTTGAAG+TGG + chr7.2:8332753-8332772 MS.gene050327:CDS 50.0%
TATCCCATCTTGGGTCTGCT+AGG + chr7.2:8332788-8332807 MS.gene050327:CDS 50.0%
! TGGGTAGCTGGACTTTTGGA+AGG + chr7.2:8333837-8333856 MS.gene050327:CDS 50.0%
! TGTTAGGCGCTGCAATCCTT+TGG - chr7.2:8332968-8332987 None:intergenic 50.0%
CATCCCTAGCAGACCCAAGA+TGG - chr7.2:8332795-8332814 None:intergenic 55.0%
TTCGGCTCAACACCCGAGAT+AGG - chr7.2:8333162-8333181 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 8332751 8334010 8332751 ID=MS.gene050327
chr7.2 mRNA 8332751 8334010 8332751 ID=MS.gene050327.t1;Parent=MS.gene050327
chr7.2 exon 8332751 8334010 8332751 ID=MS.gene050327.t1.exon1;Parent=MS.gene050327.t1
chr7.2 CDS 8332751 8334010 8332751 ID=cds.MS.gene050327.t1;Parent=MS.gene050327.t1
Gene Sequence

>MS.gene050327

ATGGAGGATCTTCCTGTTGAAGTGGTCGGTATCATATTATCCCATCTTGGGTCTGCTAGGGATGTTCTGATTGCATCCTTAACTTGCAAGAAATGGAGAGAAGCTTGGCAGAATCACCTCCAAACTCTTTCATTCAATACATCTGACTGGCCCCTATATCGCGAAATTTGCTCTAGTCACTTGGAAATGCTCATTACTTGTACACTTTTTCAAACCAAAGGATTGCAGCGCCTAACAATTTTCATGGATGATGAGCATGAGTTTTCTGTTACCCCTGTCATTGCTTGGCTTATGTATACGAGGGATTCATTGCGCGAGCTACGTTATAATGTGAGAACATCCCCTAATTTCAATATCATTGAGAAATGCAGCAGGAAAAAGTTGGAGGTTTTGACCTTGGCTAGCAATCCTATCTCGGGTGTTGAGCCGAAGTATCATAAATTTCCTTGCTTGAAGTCGCTGTCGTTGAGTTTTGTTAGCATCTCAGCGTTGGATTTGAGTCTTCTGCTTTCTGCTTGTCCGAAACTTGAAACCTTGTCTATTGTTTGTCCAGAAATCGCCATGTCAGATTCAGAGGCTTCAATAGAGCTTAGCAGCTCTTCTTTGAAGGATTTCTTTGTCGAGTCATATAGTTTTGATAAACTCATATTAGTGGCGGATATGCTCGAGAATCTGCATCTGAAAGATTGTTCCTTTGATGCTTTTGAATTGATCAATAAGGGGACATTGAAAGTATTGAAGCTTGATGATGTTAGTGTCATCCATCTTGACATTGGAGATAATACAGAAAACCTTGAGATCGTAGATGTGTGTAACTTCATATTTATGTGGCAAAATTTTTATAATATGATCTCAAAAGCGTCAAAGTTAAAAAAGCTTCGGCTGTGGAGTGTTGTTTTTGACGACGAGGATGAGGTTGTTGATATAGAGACCATATCTGTTTGTTTTCCTAGATTAACGCACCTTTCCTTAAGTTATGACTTGAAAGATGGAGTGCTTCATTATGGCTTACAAGGGTTGTCTTTTCTGATGAATGTGGTTGTGTTAGAACTTGGATGGACTACAATCAGTGATCTTTTCTCAGTTTGGGTAGCTGGACTTTTGGAAGGATGTCCTAATCTTAAAAAGATGGTCATTTATGGCTATGTTGCTGAGATCAAAACACATGAAGAGTGTCAAACTTTTACCAAATTCACTGAATTCATGATTCAACTAGGAAGAAAATATAGTCATATAAAGTTCGAGTTTGAATATGAGTAG

Protein sequence

>MS.gene050327.t1

MEDLPVEVVGIILSHLGSARDVLIASLTCKKWREAWQNHLQTLSFNTSDWPLYREICSSHLEMLITCTLFQTKGLQRLTIFMDDEHEFSVTPVIAWLMYTRDSLRELRYNVRTSPNFNIIEKCSRKKLEVLTLASNPISGVEPKYHKFPCLKSLSLSFVSISALDLSLLLSACPKLETLSIVCPEIAMSDSEASIELSSSSLKDFFVESYSFDKLILVADMLENLHLKDCSFDAFELINKGTLKVLKLDDVSVIHLDIGDNTENLEIVDVCNFIFMWQNFYNMISKASKLKKLRLWSVVFDDEDEVVDIETISVCFPRLTHLSLSYDLKDGVLHYGLQGLSFLMNVVVLELGWTTISDLFSVWVAGLLEGCPNLKKMVIYGYVAEIKTHEECQTFTKFTEFMIQLGRKYSHIKFEFEYE