AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49874


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49874.t1 MTR_4g016510 96.262 107 4 0 1 107 163 269 1.45e-71 214
MS.gene49874.t1 MTR_7g068530 67.021 94 30 1 1 94 161 253 1.06e-42 140
MS.gene49874.t1 MTR_7g068460 64.516 93 33 0 1 93 188 280 4.54e-40 134
MS.gene49874.t1 MTR_7g068550 60.638 94 37 0 1 94 161 254 3.43e-39 131
MS.gene49874.t1 MTR_3g070460 62.766 94 35 0 1 94 163 256 6.63e-38 128
MS.gene49874.t1 MTR_7g068500 61.290 93 36 0 1 93 187 279 4.79e-37 126
MS.gene49874.t1 MTR_7g068470 61.290 93 35 1 1 93 153 244 3.89e-36 123
MS.gene49874.t1 MTR_7g068470 61.290 93 35 1 1 93 157 248 4.38e-36 123
MS.gene49874.t1 MTR_7g068450 57.895 95 39 1 1 94 161 255 4.81e-36 123
MS.gene49874.t1 MTR_4g016500 86.207 58 8 0 50 107 383 440 1.09e-29 110
MS.gene49874.t1 MTR_7g068520 60.870 69 27 0 25 93 85 153 6.31e-24 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49874.t1 AT3G11210 60.920 87 34 0 1 87 163 249 4.11e-35 121
MS.gene49874.t1 AT2G38180 51.064 94 37 3 1 92 161 247 9.03e-26 98.2

Find 27 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCCATTCAAAATGTTTC+TGG 0.167567 4.2:+81154973 None:intergenic
TGACAGCTGATTCTTCAAAT+TGG 0.273635 4.2:+81154912 None:intergenic
GAATCTTCTGCAAACTCATT+TGG 0.330143 4.2:+81155056 None:intergenic
GTGATGACCATTCGATGGTT+TGG 0.411304 4.2:+81154935 None:intergenic
TCTATAGCAACTGGATCATA+TGG 0.419003 4.2:+81155032 None:intergenic
CACTTCAGCAAAGGGATGAT+TGG 0.428841 4.2:-81155446 MS.gene49874:CDS
ATTGATTTCCAGTGTAGACT+TGG 0.433496 4.2:+81155080 None:intergenic
CTACTGTCAATGTCTCCAAC+TGG 0.451353 4.2:-81155001 MS.gene49874:CDS
GACAGCTGATTCTTCAAATT+GGG 0.456804 4.2:+81154913 None:intergenic
GGTTCCAGTCGGCGTCTTCG+AGG 0.470091 4.2:+81155102 None:intergenic
CTGGTCTGCACTTCAGCAAA+GGG 0.483151 4.2:-81155454 MS.gene49874:CDS
AGGTCCTCGAAGACGCCGAC+TGG 0.497251 4.2:-81155106 MS.gene49874:CDS
ATGTCAAGGCCATTGACCTC+TGG 0.499532 4.2:-81155473 MS.gene49874:CDS
TTGATTTCCAGTGTAGACTT+GGG 0.516004 4.2:+81155081 None:intergenic
TCTGGTCTGCACTTCAGCAA+AGG 0.543201 4.2:-81155455 MS.gene49874:CDS
TGGAATTCATTTGTCACATG+AGG 0.558725 4.2:-81155159 MS.gene49874:CDS
AAGTGCAGACCAGAGGTCAA+TGG 0.560897 4.2:+81155464 None:intergenic
TGCATTTCCAAACCATCGAA+TGG 0.568329 4.2:-81154942 MS.gene49874:CDS
AGTTGTGAAAGAGATATTGA+AGG 0.577845 4.2:-81155126 MS.gene49874:CDS
ATTGGGTGATGACCATTCGA+TGG 0.584846 4.2:+81154930 None:intergenic
ATGTTTCTGGAAGTTCCAGT+TGG 0.594171 4.2:+81154986 None:intergenic
ACTGGAACCCAAGTCTACAC+TGG 0.596478 4.2:-81155088 MS.gene49874:CDS
GGAATTCATTTGTCACATGA+GGG 0.607224 4.2:-81155158 MS.gene49874:CDS
GTGTAGACTTGGGTTCCAGT+CGG 0.629499 4.2:+81155091 None:intergenic
TTTGCTGAAGTGCAGACCAG+AGG 0.667155 4.2:+81155457 None:intergenic
TACTTACGTGAAGCAAACAT+CGG 0.672886 4.2:+81155422 None:intergenic
GTGCCATGAGATGAATGTCA+AGG 0.713777 4.2:-81155487 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAATACTATAGAAAAATG+TGG + chr4.2:81155133-81155152 None:intergenic 20.0%
!!! CACATTTTTCTATAGTATTA+AGG - chr4.2:81155131-81155150 MS.gene49874:CDS 20.0%
!!! TTGTCTTGATACATTTTATA+GGG - chr4.2:81155220-81155239 MS.gene49874:intron 20.0%
! AAAATGTGGTTAAAAAATGC+TGG + chr4.2:81155119-81155138 None:intergenic 25.0%
! CTGGTAAAATAATGCTTATA+CGG + chr4.2:81155100-81155119 None:intergenic 25.0%
!! ATCACATGTGTCTTATTAAA+AGG - chr4.2:81155190-81155209 MS.gene49874:intron 25.0%
!! CTTGTCTTGATACATTTTAT+AGG - chr4.2:81155219-81155238 MS.gene49874:intron 25.0%
AGTTGTGAAAGAGATATTGA+AGG - chr4.2:81155277-81155296 MS.gene49874:intron 30.0%
TTTCCCATTCAAAATGTTTC+TGG + chr4.2:81155433-81155452 None:intergenic 30.0%
! ACTTCCAGAAACATTTTGAA+TGG - chr4.2:81155426-81155445 MS.gene49874:intron 30.0%
! CTTCCAGAAACATTTTGAAT+GGG - chr4.2:81155427-81155446 MS.gene49874:intron 30.0%
! CTTGATACATTTTATAGGGA+TGG - chr4.2:81155224-81155243 MS.gene49874:intron 30.0%
ACACAAGAACATGATATTGC+AGG + chr4.2:81155069-81155088 None:intergenic 35.0%
GAATCTTCTGCAAACTCATT+TGG + chr4.2:81155350-81155369 None:intergenic 35.0%
GGAATTCATTTGTCACATGA+GGG - chr4.2:81155245-81155264 MS.gene49874:intron 35.0%
TACTTACGTGAAGCAAACAT+CGG + chr4.2:81154984-81155003 None:intergenic 35.0%
TCTATAGCAACTGGATCATA+TGG + chr4.2:81155374-81155393 None:intergenic 35.0%
TGGAATTCATTTGTCACATG+AGG - chr4.2:81155244-81155263 MS.gene49874:intron 35.0%
TTGATTTCCAGTGTAGACTT+GGG + chr4.2:81155325-81155344 None:intergenic 35.0%
! ATTGATTTCCAGTGTAGACT+TGG + chr4.2:81155326-81155345 None:intergenic 35.0%
! GTCTTTTCATCTATAGCAAC+TGG + chr4.2:81155383-81155402 None:intergenic 35.0%
!! ATAGTTCTGAATCACAACTG+TGG + chr4.2:81155169-81155188 None:intergenic 35.0%
TGCATTTCCAAACCATCGAA+TGG - chr4.2:81155461-81155480 MS.gene49874:CDS 40.0%
!! ATGTTTCTGGAAGTTCCAGT+TGG + chr4.2:81155420-81155439 None:intergenic 40.0%
ATTGGGTGATGACCATTCGA+TGG + chr4.2:81155476-81155495 None:intergenic 45.0%
CACTTCAGCAAAGGGATGAT+TGG - chr4.2:81154957-81154976 MS.gene49874:CDS 45.0%
CTACTGTCAATGTCTCCAAC+TGG - chr4.2:81155402-81155421 MS.gene49874:intron 45.0%
GTGATGACCATTCGATGGTT+TGG + chr4.2:81155471-81155490 None:intergenic 45.0%
AAGTGCAGACCAGAGGTCAA+TGG + chr4.2:81154942-81154961 None:intergenic 50.0%
ACTGGAACCCAAGTCTACAC+TGG - chr4.2:81155315-81155334 MS.gene49874:intron 50.0%
ATGTCAAGGCCATTGACCTC+TGG - chr4.2:81154930-81154949 MS.gene49874:CDS 50.0%
CTGGTCTGCACTTCAGCAAA+GGG - chr4.2:81154949-81154968 MS.gene49874:CDS 50.0%
GTGTAGACTTGGGTTCCAGT+CGG + chr4.2:81155315-81155334 None:intergenic 50.0%
TCTGGTCTGCACTTCAGCAA+AGG - chr4.2:81154948-81154967 MS.gene49874:CDS 50.0%
TTTGCTGAAGTGCAGACCAG+AGG + chr4.2:81154949-81154968 None:intergenic 50.0%
AGGTCCTCGAAGACGCCGAC+TGG - chr4.2:81155297-81155316 MS.gene49874:intron 65.0%
GGTTCCAGTCGGCGTCTTCG+AGG + chr4.2:81155304-81155323 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 81154926 81155499 81154926 ID=MS.gene49874
chr4.2 mRNA 81154926 81155499 81154926 ID=MS.gene49874.t1;Parent=MS.gene49874
chr4.2 exon 81155429 81155499 81155429 ID=MS.gene49874.t1.exon1;Parent=MS.gene49874.t1
chr4.2 CDS 81155429 81155499 81155429 ID=cds.MS.gene49874.t1;Parent=MS.gene49874.t1
chr4.2 exon 81154926 81155184 81154926 ID=MS.gene49874.t1.exon2;Parent=MS.gene49874.t1
chr4.2 CDS 81154926 81155184 81154926 ID=cds.MS.gene49874.t1;Parent=MS.gene49874.t1
Gene Sequence

>MS.gene49874

ATGAATGTCAAGGCCATTGACCTCTGGTCTGCACTTCAGCAAAGGGATGATTGGTCCGATGTTTGCTTCACGTAAGTACTTGTTTACGACAATTTCTATTACATACAAGTCTTAAAAGTAGATGCACGTCAGAAATTGCACCTGCAATATCATGTTCTTGTGTTTTTACAGCCGTATAAGCATTATTTTACCAGCATTTTTTAACCACATTTTTCTATAGTATTAAGGATCGCGATGCAACCACAGTTGTGATTCAGAACTATGATCACATGTGTCTTATTAAAAGGCTCAAACTTGTCTTGATACATTTTATAGGGATGGAATTCATTTGTCACATGAGGGTAGCAAGATAGTTGTGAAAGAGATATTGAAGGTCCTCGAAGACGCCGACTGGAACCCAAGTCTACACTGGAAATCAATGCCAAATGAGTTTGCAGAAGATTCGCCATATGATCCAGTTGCTATAGATGAAAAGACTACTGTCAATGTCTCCAACTGGAACTTCCAGAAACATTTTGAATGGGAAAAAGCGTTGTGCATTTCCAAACCATCGAATGGTCATCACCCAATTTGA

Protein sequence

>MS.gene49874.t1

MNVKAIDLWSALQQRDDWSDVCFTDGIHLSHEGSKIVVKEILKVLEDADWNPSLHWKSMPNEFAEDSPYDPVAIDEKTTVNVSNWNFQKHFEWEKALCISKPSNGHHPI